Omega-3 Fatty Acid Retention and Oxidative Stability of Spray-Dried Chia–Fish-Oil-Prepared Microcapsules

: Background: Omega-3 fatty acids are essential fatty acids that the body cannot create itself; thus, they must be obtained from functional foods. Therefore, the food processing industries are becoming more interested in the production of omega-3 enriched food products, as consumers and healthcare organizations are increasingly demanding functional foods with minimal fatty acid loss and higher oxidative stability. Moreover, the stability of long-chain, polyunsaturated fatty acids in functional foods is a major challenge for the food processing industries. Therefore, the spray drying method was used to prepare spray-dried microcapsules (SDMs) with the minimum loss of, and more stable, fatty acids. Methods: In this study, emulsion blends of chia seed oil (CSO, 50%) and ﬁsh oil (FO, 50%) were spray-dried using varied operating conditions, including the inlet air temperature (IAT) (125, 140, 155, 170, and 185 ◦ C), wall material (WM) (5, 10, 15, 20, and 25%), pump speed (PS) (3, 4, 5, 6, and 7 mL/min), and needle speed (NS) (3, 5, 7, 9, and 11 S). Results: The maximum loss of ALA in the spray-dried microcapsules (SDMs) was observed (9.90 ± 0.40%) at 170 ◦ C, and the minimum loss was 4.18 ± 0.20% in run order 9. A similar trend was observed in the maximum retention loss of EPA and DHA (9.71 ± 0.39% and 9.77 ± 0.39%) at a high temperature of 170 ◦ C, while the minimum losses of EPA and DHA were observed in run order 9. Furthermore, the maximum peroxide value (PV) of the SDMs was observed at a lower temperature of 140 ◦ C (1.45 ± 0.19 meq O 2 /kg), and the minimum PV was 1.33 ± 0.16 meq O 2 /kg. Conclusions: Overall, based on the results, we concluded that the oxidative stability of the SDMs was improved and that it can be used as a fortifying agent in the processing of many food products.


Introduction
Over the past decade, scientists have focused their research intentions on balancing the omega-3 (ω-3) and omega-6 (ω-6) fatty acids in the human diet. Healthcare organizations have also issued nutritional recommendations for balancing the essential fatty acids in foods processed at different industrial stages. Moreover, research scientists are also exploring new combinations of omega-3 fatty acid sources to optimize the intake of essential fatty acids through the diet on daily basis. For this purpose, the terrestrial edible seed oils

Chia Seed Oil Extraction
The mini oil presser model 6YL-550 (Wuhan Acme Agro-Tech Co., Ltd, Zhengzhou, China) was used to extract the oil from the chia seeds. The graphic diagram of the mini oil presser below (Figure 1) represents the oil extraction method. In this study, the oil was extracted from chia seeds following the procedure given in the research of Rahim et al. [22]. In this process, the chia seeds were poured into the squeeze chamber through a hopper. During this process, a great deal of friction was produced between the raw materials, aiding in thermal protein denaturation and improving the oil extraction rate. After extracting the chia seed oil (CSO), the sedimentation method was used to remove the suspended solids from the oil. The extracted CSO was purified using a series of processes involving various steps, such as degumming, neutralization, bleaching, and deodorization, in order to obtain a high-quality CSO [23]. These processes were also carried out in laboratory conditions. For the further processing, the CSO was sealed and labeled in screw-cap plastic bottles and kept at room temperature for 24 h.
Chia seeds (Gazala's Pantry, CS) and fish fillets (Labeo rohita) were procured from a registered super store in Faisalabad, Punjab, Pakistan. Organic and inorganic substances were removed from the surface of the high-quality raw materials. In this research, fish (Labeo rohita) of a length of 1 m and weight of about 2 kg were used to extract the oil.

Chia Seed Oil Extraction
The mini oil presser model 6YL-550 (Wuhan Acme Agro-Tech Co., Ltd, Zhengzhou, China) was used to extract the oil from the chia seeds. The graphic diagram of the mini oil presser below (Figure 1) represents the oil extraction method. In this study, the oil was extracted from chia seeds following the procedure given in the research of Rahim et al. [22]. In this process, the chia seeds were poured into the squeeze chamber through a hopper. During this process, a great deal of friction was produced between the raw materials, aiding in thermal protein denaturation and improving the oil extraction rate. After extracting the chia seed oil (CSO), the sedimentation method was used to remove the suspended solids from the oil. The extracted CSO was purified using a series of processes involving various steps, such as degumming, neutralization, bleaching, and deodorization, in order to obtain a high-quality CSO [23]. These processes were also carried out in laboratory conditions. For the further processing, the CSO was sealed and labeled in screw-cap plastic bottles and kept at room temperature for 24 h.

Fish Oil (FO) Extraction
Oil from ground fish by-product was obtained using the solvent extraction method according to the procedure given in the research of Quero-Jiménez et al. [24], with minor modifications. For the oil extraction, a mixture of solvent (100 mL of methanol) and chloroform (50 mL) was prepared under optimal conditions. A total of 50 g of ground fish sample was dipped in the prepared solution and left overnight (7 p.m.-11 a.m.). After that, the solvent was evaporated at 40 °C using a rotary evaporator. The remaining portion was the oil extracted from the ground fish samples. The extracted FO was purified using a series of processes involving various steps, including degumming, neutralization, bleaching, and deodorization, so as to obtain a high-quality FO. For the further processing, the FO was sealed in screw-cap plastic bottles and placed in a dark room at 25 °C for 24 h.

Fish Oil (FO) Extraction
Oil from ground fish by-product was obtained using the solvent extraction method according to the procedure given in the research of Quero-Jiménez et al. [24], with minor modifications. For the oil extraction, a mixture of solvent (100 mL of methanol) and chloroform (50 mL) was prepared under optimal conditions. A total of 50 g of ground fish sample was dipped in the prepared solution and left overnight (7 P.M.-11 A.M.). After that, the solvent was evaporated at 40 • C using a rotary evaporator. The remaining portion was the oil extracted from the ground fish samples. The extracted FO was purified using a series of processes involving various steps, including degumming, neutralization, bleaching, and deodorization, so as to obtain a high-quality FO. For the further processing, the FO was sealed in screw-cap plastic bottles and placed in a dark room at 25 • C for 24 h.

Fatty Acid Composition
According to the method of Kostik et al. [25], the methyl esters of fatty acids were determined in the samples using a gas chromatography (GC) device. Furthermore, the ester of the oil was also analyzed by a gas chromatograph device according to the AOCS [26] method no. Ce 1f-96. In this method, the gas flow velocity and temperature were adjusted to 20 to 25 mL per min at 185 • C to determine the fatty acid composition of the oil. For the Processes 2022, 10, 2184 4 of 17 GC analysis, the sample was mixed thoroughly in n-hexane for 2 min at room temperature. After that, the hexane was separated and moved into the derivation tube. Then, the prepared solution was dried with nitrogen bursts. In screw-cape test tubes, 2 mL of solution was prepared by mixing sodium hydroxide (NaOH) in methanol, and the tubes were tightly sealed. After that, the solution was heated at 90 • C for 5 to 6 min and kept at room temperature until cooling. After cooling, a 2 ml solution of boron trifluoride (BF 3 ) with methanol was mixed well into the heated solution, and it was again heated at 90 • C for half an hour. After heating, the solution was again kept at room temperature for cooling. After being left to cool, the surface of the solution was separated for the GC analysis. Then, 11.6 g/mL of standard methanol and ethanol were GC-injected as sample solvents, showing the broad peak shapes of long-chain fatty acids due to the polarity mismatch between the sample solvents and the stationary phase. The peak shapes of all the fatty acids under investigation were enhanced by using standard isopropanol as the sample solvent. This method was specifically applied to estimate the fatty acid profile of the oil. We calculated the peak value by estimating the concentration percentage.

Free Fatty Acids (FFAs)
The concentration of FFAs in the oils and their blends were evaluated using the Ca 5a-40 method, as explained in AOCS [27]. Typically, 1 g of oil sample was mixed into 25 mL of ethyl alcohol, followed by the dissolution of 50 µL of phenolphthalein indicator. After that, the prepared solution was titrated with 0.01 N of NaOH until a faint, permanent pink color appeared.

Peroxide Value (PV)
The PV was determined in the samples using the official method 965.33 [28]. A Spectrophotometric method was used to determine lipid peroxidation, as presented in the research of Low [29] and Scheme [30]. The conjugated double bonds were measured following the procedure described by Hamilton [31].

Preparation of Emulsion
For the spray drying process, the oil blend of fish (50%) and CSO (50%) oil was prepared under optimum processing conditions, according to the recent research of Rahim et al. [22]. A hydrated solution of emulsion continuous phase was prepared by dissolving gum arabic (GA) and maltodextrin (MD) in distilled water using a high-speed blender. A total of 500 mL of emulsion was prepared with 20% chia-fish oil blend. After that, soy lecithin was used as an emulsifier and added to the solution at the concentration of 1:1 as a mass ratio for all the chia-fish oil blend formulations, followed by stirring for 20 min. After mixing, the prepared solution was homogenized using a high-speed homogenizer (FSH-2A, Changzhou, China) at 10,000 rpm for 5 min.

Optimization of Spray-Dried Microcapsules (SDM)
The fatty acid composition, FFAs, and PV of the oil samples extracted from the SDM samples were observed according to the procedures described in the previous methodology description, Section 2.4, respectively.

Statistical Analysis
The results of this research were calculated using the central composite design (CCD) and response surface methodology, applying the optimized conditions of the spray dryer. The coded levels of the points of the factorial design were −2, −1, 0, +1, +2, and using the design of the experiment techniques, output variables (responses) were optimized with respect to the input variables (factors such as the IAT, WM, PS, and NS). Stat-Ease ® and Matlab Software ® were used to determine the level of significance and predict the Rsquared value so as to determine the validity of the developed model's calculation of the dependent and independent variables [33].

Fatty Acid Composition of the Extracted Oil
The chia seed oil (CSO) was extracted using the mini oil presser model 6YL-550. The extraction of CSO by the cold-pressed method involves many unit operations, in which oil extraction is a very important step, as it measures the quality and quantity of the extracted oil [34]. In addition, numerous studies aiming to extract good-quality oil from ground fish with a maximum efficiency and yield have been conducted around the world,

Optimization of Spray-Dried Microcapsules (SDM)
The fatty acid composition, FFAs, and PV of the oil samples extracted from the SDM samples were observed according to the procedures described in the previous methodology description, Section 2.4, respectively.

Statistical Analysis
The results of this research were calculated using the central composite design (CCD) and response surface methodology, applying the optimized conditions of the spray dryer. The coded levels of the points of the factorial design were −2, −1, 0, +1, +2, and using the design of the experiment techniques, output variables (responses) were optimized with respect to the input variables (factors such as the IAT, WM, PS, and NS). Stat-Ease ® and Matlab Software ® were used to determine the level of significance and predict the R-squared value so as to determine the validity of the developed model's calculation of the dependent and independent variables [33].

Fatty Acid Composition of the Extracted Oil
The chia seed oil (CSO) was extracted using the mini oil presser model 6YL-550. The extraction of CSO by the cold-pressed method involves many unit operations, in which oil extraction is a very important step, as it measures the quality and quantity of the extracted oil [34]. In addition, numerous studies aiming to extract good-quality oil from ground fish with a maximum efficiency and yield have been conducted around the world, but the solvent extraction method has been found to be the most effective method. The solvent extraction method produces high-quality FO with a maximum yield or efficiency [29,35].
The fatty acid composition is an important attribute for measuring the quality of chia and fish oil. The results showed that the FO samples contained 3.40 ± 0.18% of EPA, 18.33 ± 0.34% of DHA, 7.1 ± 0.22% of FFAs, and 3.15 ± 0.29 meq O 2 /kg PV, as presented in Table 2. On the other hand, the CSO samples contained 62.22 ± 0.45% of α-Linolenic acid (ALA), 1.7 ± 0.12% of free fatty acids (FFAs), and 1.65 ± 0.10 meq O 2 /kg PV, while not containing EPA (eicosapentaenoic acid) or DHA (docosahexaenoic acid). Similarly, the chia and fish oil blend samples contained ALA (31.3 ± 0.64%), EPA (2.34 ± 0.23%), DHA (8.9 ± 0.38%), FFAs (2.1 ± 0.21%), and PV (2.06 ± 0.18 meq O 2 /kg), respectively, and were within the acceptable range for edible oil. The spray-dried microcapsules (SDMs) were optimized using the central composite design (CCD), enabling a total of 30 experiments to be performed in order to obtain the factor effects on the ALA, EPA, and DHA (% of TFA) loss. All runs were randomized in order to avoid systematic errors. The mutual interaction effects between the spray dryer parameters related to the percent loss of ALA, EPA, and DHA for the SDMs were determined by rotating four independent factors, including the IAT ( • C), WM (%), PS (mL/min), and NS (S), as described in Table 3, respectively. Table 3. Optimization of the spay drying operating conditions for the spray-dried microcapsules.    In this study, the combined effects of factors affecting the fatty acid composition of the SDMs showed that the minimum ALA retention loss was 4.18 ± 0.20% at 140 • C, 10 mL/min, 4%, and 5S. Furthermore, the greatest ALA content loss, obtained in run order 26, was 9.90 ± 0.40% at the high temperature of 170 • C. The contour plots of the ALA loss indicated the mutual interactions between operating factors, as presented in Figure 3. Furthermore, the lowest values of the EPA and DHA content loss in the microcapsules were calculated in run order 9 (5.71 ± 0.20% EPA and 0.44 ± 0.10% DHA), while the highest loss was measured in run order 26 (9.71 ± 0.39% EPA and 9.77 ± 0.39% DHA). The EPA and DHA retention losses were in the ranges from 5.71 to 9.71% and 0.44 to 9.77%, respectively. Figures 4 and 5, showing the surface and contour plots, indicate the interaction effects of two independent variables on the EPA and DHA loss. Moreover, the maximum value of FFAs was 0.72 ± 0.15% in run order 9, and the minimum value of FFAs measured in run order 26 was 0.67 ± 0.11%. In addition, the surface plots of the FFAs indicated the mutual interactions between operating factors, as presented in Figure 6. At the end of the experiment, the results indicated that spray drying did not greatly affect the fatty acid composition of the SDMs. As shown in Table 3, the percentages of ALA, EPA, and DHA were similar to those of natural oil, showing only modest variation in the fatty acid composition in the SDMs. An ANOVA was conducted and revealed that the models were significant and that the lack of fit was not significant ( Table 4). The significance and the magnitude of the estimated coefficients of each variable and all their possible linear interactions and quadratic effects of the response variables were estimated using the Design Expert Software.

Oxidative Stability
Oxidative stability is also known as the oil stability index, which measures the length of time before the oxidation process begins. Several factors are affected during oil processing and storage. An important factor is lipid oxidation, which has direct effects on the oil quality, flavor, odor, nutritional value, and loss of essential fatty acids and minimizes the shelf life during storage. Lipid oxidation is a process that is also called the rancidity of oil, in which oxygen produces free radicles in the cell membrane that destroy the cell

Oxidative Stability
Oxidative stability is also known as the oil stability index, which measures the length of time before the oxidation process begins. Several factors are affected during oil processing and storage. An important factor is lipid oxidation, which has direct effects on the oil quality, flavor, odor, nutritional value, and loss of essential fatty acids and minimizes the shelf life during storage. Lipid oxidation is a process that is also called the rancidity of oil, in which oxygen produces free radicles in the cell membrane that destroy the cell membrane [36]. In this study, a fish and chia oil blend emulsion was prepared for subjection to a spray drying process in order to improve the oxidative stability of omega-3-enriched oil samples. Chia seed oil (CSO) has a lower oxidative stability than FO due to the differences in their fatty acid compositions, oil extraction methods, and the number of phytochemical compounds. In this research, the maximum peroxide value (PV) of the SDMs was found to be 1.45 ± 0.19 meq O 2 /kg in run order 9. Furthermore, the lowest PV value was measured in run order 26 (1.33 ± 0.16 meq O 2 /kg). The results showed that the oxidative stability of the SDMs was significantly improved, as compared with the raw oil blend sample. Finally, it was observed that an increase in the amount of wall material and the inlet temperature significantly increased the PV of the SDMs, as described in Table 3. The mutual interaction effects of the independent factors on the PV of the SDMs is also explained in Figure 7.

Model Fitting
Modified SDMs containing a chia and fish oil blend were produced by the spray drying method under optimal processing conditions. After conducting all the experiments, the best fitting model was determined via the regression equations described in Table 5. Regression equations for the specific levels of each factor can predict the response variable, in which the factors' coded and actual levels are coded as −2 and +2, respectively. Furthermore, five coded levels of a CCD were used for the reactions between the independent variables and dependent variables. Our results were fitted to the quadratic polynomial model. This model shows the relationship between the dependent variables and the independent variables, with four independent factors. In this model, the predicted R 2 value was estimated to be in the range from 0.1047 to 0.6609, with a small fraction of this response variable ranging from 0.6095 to 0.8694 with the adjusted R 2 , as can be expected in general. Moreover, the lack of fit F-value ranged from 0.92 to 10.16, results which were significant and non-significant relative to the pure error described in Table 4.

Model Fitting
Modified SDMs containing a chia and fish oil blend were produced by the spray drying method under optimal processing conditions. After conducting all the experiments, the best fitting model was determined via the regression equations described in Table 5. Regression equations for the specific levels of each factor can predict the response variable, in which the factors' coded and actual levels are coded as −2 and +2, respectively. Furthermore, five coded levels of a CCD were used for the reactions between the independent variables and dependent variables. Our results were fitted to the quadratic polynomial model. This model shows the relationship between the dependent variables and the independent variables, with four independent factors. In this model, the predicted R 2 value was estimated to be in the range from 0.1047 to 0.6609, with a small fraction of this response variable ranging from 0.6095 to 0.8694 with the adjusted R 2 , as can be expected in general. Moreover, the lack of fit F-value ranged from 0.92 to 10.16, results which were significant and non-significant relative to the pure error described in Table 4. Table 5. Coded and actual regression equations for the response factors according to the spray drying conditions.

Regression Form Regression Equations
ALA loss (%)

Discussion
The present study verified that the fatty acid composition of fish and chia oil is influenced by the effects of factors such as the quality of the seed, weather conditions, storage temperature, and extraction system [37]. In a work by Bruno et al. [38], the results show that the head oil of labeo rohita contained 0.86% of EPA and 0.15% of DHA. Ghosh and Bhattacharyya [9] reported that the concentrations of FFAs and PV in FO increased significantly with increasing temperature. Moreover, in a similar study conducted by the Ixtaina et al. [39], screw-pressed CSO contained 64.5% ALA. In another similar work, the CSO contained 59.76% linolenic acid at 90 • C and 20.13% linoleic acid at 90 • C [40]. Another study found that the CSO was rich in ALA (61.1%) and linoleic acid (16.6%) [41]. According to the Ghena and MB [42], CSO contained 60.69% of ALA (unsaturated fatty acid). In another work, the fatty acid profile showed that the CSO contained 18.11 mg/g ALA [43]. In a similar study carried out by Carrillo et al. [44], the ALA in CSO was 54.08%. In addition, low PVs of 1.08-2.56 meq O 2 /kg have been noted in CSO samples [45,46]. In a similar work by Ghosh et al. [47], the highest PV was noted in samples of the blended oil containing FO and CSO in the ratio of 2:1, which was within the acceptable range for edible oil.
Most importantly, omega-3 and omega-6 have different benefits for human health. These LCPUFAs are essential for animal and human physiology due to their structural and regulatory functions. These valuable essential fatty acid components play vital roles in preventing or inhibiting health risks such as heart disease, chronic cancer, inflammation, and dementia [48,49]. Moreover, these essential omega-3 fatty acids are very important for cell repair and development, the body composition, and the function of the human body. These fatty acids play a very important role in the chemical reactions of the metabolism process. However, the intake of the wrong balance of essential fatty acids causes several of diseases in human body [2]. According to health services, a daily intake of EPA and DHA of 150 to 500 mg/day can reduce the triglyceride levels and the risk of blood clot formation [50]. Long-chain PUFAs are essential for visual and neurological development in the human body [1]. The ω-3 and ω-6 found in FO play important roles in the development, maintenance, and function of the brain, eyes, and cardiovascular and nervous systems [51]. Gamma-linolenic acid, ω-3, and ω-6 supplementations are very effective against skin diseases such as acne, itchy and scaly patches, rashes, swollen skin, and skin irritation [52].
The fatty acid profile of SDMs is influenced by the presence of non-triglyceride compounds, temperature (during scanning), and fatty acid double-bond chains [53]. Further-more, in the research of Karthik and Anandharamakrishnan [54], it was concluded that during the preparation of emulsion for microencapsulation, temperature and physical stresses affect the fatty acid profiles of oil samples. The fatty acid profile of chia oil and seed was maintained under high-temperature spray drying operating conditions [55]. The results were in strong agreement with the research of Lavanya et al. [32] and Ali et al. [56], who also concluded that there was no significant change in the fatty acid profile of a chia and fish oil blend after increasing and decreasing the temperature in the spray drying process. In another study, the fatty acid profiles of chia seed oil and eggs were significantly decreased by increasing the temperature during spray drying, and high temperature conditions in the spray dryer caused higher losses of PUFAs [57]. Similarly, the fatty acid profile of fish oil was observed after the spray drying process at a high temperature, with no significant losses when compared to the bulk oil [58]. In addition, several recent studies have shown that the losses of LCPUFAs were increased by increasing the temperature in the spray drying processing [59,60].
Humans cannot synthesize these omega-3 fatty acids, and they must be obtained from food sources. Seed and marine oils are good natural sources of these omega-3 fatty acids. These essential fatty acids are found in large quantities in, for example, flax, rapeseed, chia, perella, walnuts, and fish meal. When the human body consumes PUFAs, these essential fatty acids are converted into oleic acid, linoleic acid, ALA, EPA, DHA, but this is a slow process. Therefore, a diet high in ω-3 and ω-6 fatty acids can be very effective against many diseases, such as hyperlipoproteinemia, atherosclerotic disease, obesity, hypertriglyceridemia, and platelet pro-aggregatory and prothrombotic diseases [61]. Therefore, many food industries have shown an increased interest in the production of PUFA-enriched functional food products [62]. Chia and fish oil by-products are an excellent natural sources of LCPUFAs, especially ALA, EPA, and DHA. Due to the presence of LCPUFAs, the stability of these oils is very low in terms of the products created in the food processing industry. The solution to this issue is the microencapsulation of oil using the various methods to increase the stability of fatty acids. However, food industries prefer to use encapsulated oils in fortified food products due to their longer shelf life, as compared to the raw oils [14,63,64]. In addition, the use of functional foods to ensure a heathy lifestyle has been increasing over the past few years [65]. Health services or organizations have also recommended that consumers consume more functional foods in which omega-3 fatty acids are balanced and less foods with unbalanced fatty acid profiles to ensure a good health and lifestyle [66].
These results are in agreement with the study of Shi et al. [67], in which the oxidative stability of spray-dried FO was improved at a low inlet temperature (140 • C) using various types of core materials (mixture of matcha and maltodextrin). Moreover, a work by Takashige et al. [68] reported that the stability of FO was increased after the spray drying of samples with maltodextrin. In another study, the oxidative stability of spray-dried fish oil microcapsules was improved during storage compared to the crude FO [69]. Furthermore, in the research of Hogan et al. [70] and Encina et al. [71], it was concluded that the PV of FO after spray drying was increased, as compared with raw oil samples. Moreover, in another work, microcapsules of CSO were prepared using a small-scale spray dryer, and the PV of the functional oil was 42.5 meq/kg and the PV of the microencapsulated oil was 10.3 meq/kg under optimal conditions. Hence, the oxidative stability of CSO was improved after spray drying [72]. A research work of Timilsena et al. [60] concluded that the PV of CSO microcapsules was improved during thermal processing and storage.
All over the world, various encapsulation processes are used to increase the stability of omega 3 fatty acids in oils. Most commonly, the spray drying process is used in the food processing industry to produce fortified products that are high in omega-3 fatty acids. This process is low in cost and easy to perform, requires less energy consumption, and produces good-quality microcapsules. Omega-3 fatty acids are essential for human health; thus consumers eat nutritious foods to keep the body healthy [73]. The fortification of food with PUFAs is considered as a good a way to increase one's dietary of these products intake and reduce the risk of many chronic diseases. Therefore, consumers consume functional foods which contain PUFAs because these fatty acids play a very important role in the development of brain functions, reduce the risk of cardiovascular diseases, and boost the immunity of the body [8]. In another study, FO was deemed to be the main source of EPA and DHA, which have positive impacts in controlling the cholesterol levels and lowering the blood pressure. Several studies have claimed that FO reduces the risk of many types of cancer. Therefore, functional food products are developed as microcapsules of EPA and DHA for those who do not eat fish because they cannot purchase fish. These omega-3enriched products influence several risk factors, such as bad cholesterol levels, and they can also control the blood pressure, platelet aggregation, and triglyceride levels [74].

Conclusions
Chia and fish oil are high in ALA, EPA, and DHA, which are essential for the human body and protect it against many diseases. In this study, GA and MD were used as wall materials in the preparation of emulsion for spray drying. According to the results, there was no significant loss in the fatty acid composition of the SDMs, with a retention of 94 ± 5% after spray drying. Furthermore, the concentration of FFAs in the SDMs was lower compared to the blend of chia and fish oil. Finally, the PV of the SDMs indicated that the oxidative stability was higher than that of the chia and fish oil blend. Further studies should focus on the development of innovative substances and techniques to improve the process of industrial-scale encapsulation, to produce fortified products encapsulating essential oils in the future, and to provide non-lethal therapeutic agents for the treatment of numerous diseases.