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Article

Inactivated Platelet Lysate Supports the Proliferation and Immunomodulant Characteristics of Mesenchymal Stromal Cells in GMP Culture Conditions

1
Department of Public Health and Paediatrics, The University of Turin, Piazza Polonia 94, 10126 Torino, Italy
2
Stem Cell Transplantation and Cellular Therapy Laboratory, Paediatric Onco-Haematology Division, Regina Margherita Children’s Hospital, City of Health and Science of Turin, 10126 Torino, Italy
3
Blood Component Production and Validation Center, City of Health and Science of Turin, S. Anna Hospital, 10126 Turin, Italy
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Biomedicines 2020, 8(7), 220; https://doi.org/10.3390/biomedicines8070220
Received: 24 May 2020 / Revised: 2 July 2020 / Accepted: 13 July 2020 / Published: 17 July 2020
(This article belongs to the Section Model Systems in Research and Development)
Mesenchymal stromal cells (MSCs) isolated from bone marrow (BM-MSCs) are considered advanced therapy medicinal products (ATMPs) and need to be produced according to good manufacturing practice (GMP) in their clinical use. Human platelet lysate (HPL) is a good GMP-compliant alternative to animal serum, and we have demonstrated that after pathogen inactivation with psoralen, it was safer and more efficient to use psoralen in the production of MSCs following GMP guidelines. In this study, the MSCs cultivated in fetal bovine serum (FBS-MSC) or inactivated HPL (iHPL-MSC) were compared for their immunomodulatory properties. We studied the effects of MSCs on (1) the proliferation of total lymphocytes (Ly) and on naïve T Ly subsets induced to differentiate in Th1 versus Th2 Ly; (2) the immunophenotype of different T-cell subsets; (3) and the cytokine release to verify Th1, Th2, and Th17 polarization. These were analyzed by using an in vitro co-culture system. We observed that iHPL-MSCs showed the same immunomodulatory properties observed in the FBS-MSC co-cultures. Furthermore, a more efficient effect on the increase of naïve T- cells and in the Th1 cytokine release from iHPL was observed. This study confirms that iHPL, used as a medium supplement, may be considered a good alternative to FBS for a GMP-compliant MSC expansion, and also to preserve their immunomodulatory proprieties. View Full-Text
Keywords: mesenchymal stromal cells; good manufacturing practice; inactivated platelet lysate mesenchymal stromal cells; good manufacturing practice; inactivated platelet lysate
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MDPI and ACS Style

Mareschi, K.; Castiglia, S.; Adamini, A.; Rustichelli, D.; Marini, E.; Banche Niclot, A.G.S.; Bergallo, M.; Labanca, L.; Ferrero, I.; Fagioli, F. Inactivated Platelet Lysate Supports the Proliferation and Immunomodulant Characteristics of Mesenchymal Stromal Cells in GMP Culture Conditions. Biomedicines 2020, 8, 220. https://doi.org/10.3390/biomedicines8070220

AMA Style

Mareschi K, Castiglia S, Adamini A, Rustichelli D, Marini E, Banche Niclot AGS, Bergallo M, Labanca L, Ferrero I, Fagioli F. Inactivated Platelet Lysate Supports the Proliferation and Immunomodulant Characteristics of Mesenchymal Stromal Cells in GMP Culture Conditions. Biomedicines. 2020; 8(7):220. https://doi.org/10.3390/biomedicines8070220

Chicago/Turabian Style

Mareschi, Katia, Sara Castiglia, Aloe Adamini, Deborah Rustichelli, Elena Marini, Alessia G.S. Banche Niclot, Massimiliano Bergallo, Luciana Labanca, Ivana Ferrero, and Franca Fagioli. 2020. "Inactivated Platelet Lysate Supports the Proliferation and Immunomodulant Characteristics of Mesenchymal Stromal Cells in GMP Culture Conditions" Biomedicines 8, no. 7: 220. https://doi.org/10.3390/biomedicines8070220

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