Editorial for This Special Issue “Synaptic Transmission: From Molecular to Neural Network Levels”

We invited contributions reporting new evidence of synaptic mechanisms and their network-level impacts for this Special Issue [...].

results show that the circuit can efficiently process and transmit low-frequency inputs.This was a previously underestimated property of this circuit, considering the ability of granule cells to emit spikes at fast rates with sub-millisecond precision [2], which drove most cerebellar research towards faster activity rates.This evidence aligns with the oscillatory and resonant properties of the cerebellar network, the findings reported by Bauer and colleagues, and the recently revalued cerebellar role in the supra-second domain [5].Monteverdi and colleagues conclude that cerebellar dynamic processing is governed by a complex set of spatiotemporal filters characterized by a notable frequency dependence and an anisotropy that is likely to reflect the anatomical organization of local inhibition.
In the paper by Mapelli et al., another aspect of synaptic plasticity in the cerebellar granular layer was considered.By combining patch-clamp intracellular recordings and mathematical modeling, the authors show that long-term plasticity at the cerebellar input stage can alter the gain bandwidth of mossy fiber burst transmission.It is known that the mossy fiber-granule cell relay can differentially undergo LTP or LTD depending on the granule cell depolarization attained during the induction [6].The novelty of the paper by Mapelli et al. lies in the finding that long-term plasticity can tune the gain and timing of transmission along channels at the cerebellum input stage.The spatiotemporal reconfiguration of the mossy fiber-granule cell input-output transmission is a gain-controlled process dependent on long-term plasticity.Moreover, the different effects of long-term depression and potentiation strongly affect the time and frequency domains of single-transmission channels.These results confirm the hypothesis that the cerebellum can behave as an adaptive spatiotemporal filter.This spatiotemporal organization in the center-surround characteristics structures reflects a fine-tuning of the excitatory/inhibitory balance between the center (with a higher E/I ratio) and the surround (with a lower E/I ratio) due to the differential activation of glutamate and GABA receptors.The voltage-dependent NMDA receptors are regulated accordingly, enhancing burst transmission in response to their long integration time constant and inducing synaptic plasticity thanks to their calcium permeability.In the center, larger depolarizations would enhance NMDA channel voltage-dependent unblocking, improving synaptic integration at low frequencies and simultaneously raising the calcium influx, facilitating LTP induction [7].The computational model confirmed that the changes in the GABAergic inhibition-mimicking plastic mechanisms were required to attenuate the marked changes in gain curves occurring when only excitatory plasticity was present.GABAergic plasticity acts as a homeostatic regulatory mechanism avoiding the saturation of synaptic weights and maintaining a dynamic range of activity in both time and frequency domains.The authors conclude that the cerebellar granular layer activity can be reorganized by LTP and LTD tuning the firing composition in terms of spike delay and fine-tuning the number and frequency of spikes.Moreover, LTP and LTD can extend the transmission bandwidth toward lower frequencies.
Parkinson's disease (PD) is a neurodegenerative condition traditionally related to the classic motor phenotype that also shows clear cognitive symptoms [8].Though the clinical manifestations show a high degree of heterogeneity, most genes and proteins implicated in its pathogenesis are directly or indirectly related to the synaptic function, among which one of the most renowned examples is alpha-synuclein.For this reason, PD is considered a synaptopathy [9].In this Special Issue, Oey and colleagues applied quantitative proteomics and RNA interference techniques in rat primary cortical neuron cultures to describe the effects of knocking down the expression of PHF8 in synaptically activated neurons.PHF8 is a histone demethylase involved in PD and X-linked intellectual disability.This study reports that PHF8 silencing in activated neurons results in the downregulation of several proteins involved in synaptic structure, function, and plasticity, both at the pre-and postsynaptic site, including alpha-synuclein, calcium-calmodulin-dependent kinase II alpha (CaMKIIa), complexin 1, and other proteins found involved in PD.In particular, alpha-synuclein has been causally related to PD for its role in dopaminergic transmission [9], and CaMKIIa is known to play a critical role in synaptic transmission and plasticity, including dopaminergic connections [10].Interestingly, these results suggest that large clusters of functionally related proteins are affected by the downregulation of PHF8.This evidence deserves further investigation in light of its potential impact on novel approaches to disease-modifying therapies [11].
Pimozide is an antipsychotic drug known to inhibit synaptic transmission in the central nervous system, acting as a dopamine D2 receptor antagonist [12], and is, therefore, used for schizophrenia and other neurological disorder treatments [13].Pimozide is also known for its side effects, including akinesia, tremor, and altered vestibular function.In fact, blocking D2 receptors inhibits synaptic transmission, while cell depolarization produced by blocking K + channels may increase synaptic transmission.Giunta and co-authors investigated the effects of pimozide on the ionic currents of voltage-gated K + channels recorded from chicken embryo vestibular type-II hair cells using patch-clamp whole-cell recordings in the ex vivo slices of semicircular canals obtained at different developmental stages.The authors demonstrated that pimozide increased the amplitude of I KV elicited at negative potentials in vestibular type-II hair cells.This effect was still present at a lower concentration, while the already-known blocking effect disappeared (3 µM vs. 0.3 µM).Moreover, pimozide significantly hyperpolarized the resting membrane potential of type-II hair cells.This would close voltage-gated Ca 2+ channels at the basolateral side, which are involved in the glutamate exocytosis at the afferent nerve terminal of vestibular primary sensory neurons.Altogether, these results suggest that the hyperpolarization of vestibular hair cells is related to the decrease in glutamate release due to the pimozide effect.It is known that vestibular dysfunctions are very common in adults [14], and vertigo is one of the main symptoms due to abnormal signaling from vestibular hair cells.Usually, the treatment in the case of severe vestibular symptoms, such as vertigo, is the chemical or surgical ablation of the vestibular nerve, but this can lead to permanent hearing loss.Therefore, pimozide, which has no hearing loss side effects, could be a good candidate for vestibular disorder treatment.Moreover, the authors demonstrated that pimozide acts as a K + channel opener and could be a promising drug treatment also for other neurological disorders.
During the last decade, the consumption of sugar-sweetened beverages has dramatically increased, and it has been demonstrated that it can impair brain function [15].Brain-derived neurotrophic factor (BDNF) is a key molecule involved in plastic changes related to learning and memory, and its expression is highly regulated [16].In this Special Issue, Coirini et al. demonstrated that juvenile rats exposed to high-sugar beverages show reduced mRNA expressions of BDNF and hippocampus inflammation.Novel object recognition (NOR) tests were performed in juvenile and adult rats exposed to 10% sucrose beverages.The study by Coirini et al. demonstrates impairment in NOR only in juvenile rats, excluding attention deficits, as the time spent in the exploring phase was comparable in the two groups.Moreover, while the authors previously reported long-term changes in fear memory conditions and anxiety behavior in juvenile rats exposed to sucrose [17], the behavioral conditions were optimized in the present research such that the animals showed no anxiety.Therefore, the cognitive defects observed were due to learning and memory impairment and not stress interference.The authors also showed that the BDNF precursor (proBDNF) decreased in juvenile rats exposed to sucrose, while it increased in adults; the decreased proBDNF expression was observed in the hippocampus correlated to decreases in the recognition memory.The authors also found an increased expression of the receptor for advanced glycation end-products (RAGE) in adults but without a correlation with deficit cognition.In conclusion, lower levels of proBDNF in the hippocampus may be the cause of the cognitive deficits observed in adult animals exposed to sucrose during youth.

Conflicts of Interest:
The authors declare no conflicts of interest.

Author Contributions:
Conceptualization, L.M. and S.T.; writing-original draft preparation, L.M. and S.T.; writing-review and editing, L.M. and S.T.All authors have read and agreed to the published version of the manuscript.