CARD14 Signalling Ensures Cell Survival and Cancer Associated Gene Expression in Prostate Cancer Cells

Prostate cancer (PCa) is one of the most common cancer types in men and represents an increasing global problem due to the modern Western lifestyle. The signalling adapter protein CARD14 is specifically expressed in epithelial cells, where it has been shown to mediate NF-κB signalling, but a role for CARD14 in carcinoma has not yet been described. By analysing existing cancer databases, we found that CARD14 overexpression strongly correlates with aggressive PCa in human patients. Moreover, we showed that CARD14 is overexpressed in the LNCaP PCa cell line and that knockdown of CARD14 severely reduces LNCaP cell survival. Similarly, knockdown of BCL10 and MALT1, which are known to form a signalling complex with CARD14, also induced LNCaP cell death. MALT1 is a paracaspase that mediates downstream signalling by acting as a scaffold, as well as a protease. Recent studies have already indicated a role for the scaffold function of MALT1 in PCa cell growth. Here, we also demonstrated constitutive MALT1 proteolytic activity in several PCa cell lines, leading to cleavage of A20 and CYLD. Inhibition of MALT1 protease activity did not affect PCa cell survival nor activation of NF-κB and JNK signalling, but reduced expression of cancer-associated genes, including the cytokine IL-6. Taken together, our results revealed a novel role for CARD14-induced signalling in regulating PCa cell survival and gene expression. The epithelial cell type-specific expression of CARD14 may offer novel opportunities for more specific therapeutic targeting approaches in PCa.


Introduction
Most cancers arise in epithelial cells, manifesting as carcinomas in organs such as the lung, skin, breast, colon, and prostate. Prostate cancer (PCa) is one of the leading causes of cancer-related death in men worldwide, despite the fact that the overall survival rate after PCa diagnosis is relatively high [1,2], indicating the need for better treatment options. In the Western countries, PCa rates are particularly increased due to the wide occurrence of obesity, metabolic syndrome, and associated comorbidities that elevate inflammation, which has been linked to PCa susceptibility [3]. Inflammatory mediators, in turn, stimulate malignant proliferation of epithelial cells via activation of various signalling pathways, including NF-κB, ERK, or Akt [4,5]. Therefore, epithelial cell-specific signalling molecules are of particular interest for the development of new anticancer drugs.

Human PCa Data Sets
The Cancer Genome Atlas (TCGA) prostate adenocarcinoma (PRAD) gene expression data (RNA sequencing raw counts, z-score and RSEM) was downloaded from the Firehose website [28] Corresponding clinical data of patients were retrieved from the TCGA data portal [29]. Additional gene expression data (TCGA PRAD RSEM and MSKCC PRAD z-scores) and clinical data were curated from the cBioportal website [30].

Proliferation Score
The Proliferation Score or meta-PCNA index was calculated as previously described [31]. In brief, the meta-PCNA index of each sample was computed by taking the median expression of predefined genes that were found to be the top 1% most positively correlated with PCNA expression.

Gene Ontology (GO) Enrichment Analysis
We calculated the correlation between CARD14 and all the genes in the TCGA PRAD data set and selected the significant positively correlated genes (p < 0.05 and r > 0). This list of genes was queried for the enrichment of Biological Processes on the "PANTHER" website [32].

Gene Set Enrichment Analysis (GSEA)
The GSEA 4.1.3 software was downloaded from Broad Institute [33]. GSEA on TCGA PRAD gene expression data was used to discover hallmark gene sets that are significantly enriched with CARD14 expression. CARD14 was used as a continuous phenotype label to rank patients from high to low CARD14 expression. The enrichment of gene sets was tested with 1000 permutations. Gene sets with a positive enrichment score were enriched in patients with high CARD14 expression, while gene sets with a negative enrichment score were enriched in patients with low CARD14 expression.

DNA Damage Response (DDR) Pathway Activity
DDR gene list was assembled from an online catalogue of DDR genes from recently published resources [34]. This gene list was subdivided into eight major DDR pathways: the Fanconi anemia pathway, non-homologous DNA end joining, nucleotide excision repair, base excision repair, translesion DNA synthesis, damage sensor, homology-dependent recombination, mismatch repair, and direct damage reversal/repair. The complete gene list is contained in Table S1. DDR pathway activity was calculated by taking the mean expression of all the genes in that pathway.

Cells, siRNA-Mediated Knockdown and Cell Death Assays
Human prostate cancer cell lines LNCaP (ATCC CRL-1740) and PC3 (ATCC CRL-1435) were cultured in RPMI 1640 medium, supplemented with 10% fetal calf serum and 3 mM or 2 mM L-glutamine, respectively. Du145 (ATCC HTB-81) were cultured in Dulbecco's modified Eagle's medium, supplemented with 10% fetal calf serum and 2 mM Lglutamine. Immortalised normal adult prostatic epithelial cells PNT1A (ECACC 95012614) were cultured in RPMI 1640 medium, supplemented with 10% fetal calf serum, 2 mM L-glutamine and β-mercaptoethanol. INTERFERin ® (POL-409-10, Polyplus Transfection, Illkirch, France) was used for reverse transfection of 2 × 10 5 cells with 20 nM of siRNA. SMARTpool ON-TARGET plus non-targeting pool control (D-001810-10), CARD14-(L-004397-00), BCL10-(L-004381-00) or siGENOME MALT1-specific (M-005936-02) siRNA were purchased from Dharmacon (Horizon Discovery Biosciences, Cambridge, United Kingdom). 24 h later SYTOX Green nucleic acid stain (S-7020, ThermoFisher Scientific, Merelbeke, Belgium) was added to final concentration of 1 µM and cells were incubated in a CO2 and temperature-controlled environment that allowed measurement of fluorescent signals over a time span of 72 h, as previously described [35]. The number of dead cells was quantified in function of time using the Incucyte Zoom Live-Cell Analysis System (Essenbio, Newark, UK). Values were normalised against a control well that was seeded with the same number of cells as in the experimental well and imaged in parallel following treatment with Triton X-100 to fully permeabilise the cells. The maximum number of positive nuclei in these control wells was considered 100% and used to normalise values of fluorescent cells in experimental wells. Gene knockdown was verified by immunoblotting 48-72 h later.

Multiplex Antibody Array Analysis of Protein Expression
7 × 10 6 PC3 MALT1 KO or reconstituted cells were stimulated with 10 µg/mL Brefeldin A for 6 h. Cells were washed in PBS and lysed in R&D Lysis Buffer 17 (1% Igepal CA-630, 20 mM Tris-HCl (pH 8.0), 137 mM NaCl, 2 mM EDTA, 200 mM Sodium orthovanadate, 5 mM NaF). Protein concentration was measured using Bio-Rad protein assay dye reagent (5000006, Bio-Rad Laboratories, Nazareth, Belgium) and equal amounts of total lysate were analysed with Proteome Profiler Human XL Oncology Array (ARY026; R&D, Minneapolis, MN, USA) according to the manufacturer's instructions. Membranes were exposed to X-ray film, after which profiles of mean spot pixel density were quantified using image analysis software.

CARD14 Is Associated with Aggressive PCa in Human Patients
To investigate whether CBM signalling may play a role in PCa, we initially analysed whether expression of CBM components correlates with survival of patients with PCa. To this end, we explored The Cancer Genome Atlas (TCGA) database and analysed Disease/progression-free survival of PCa patients expressing high or low levels of CARD14, BCL10, and MALT1. Because the CARD14-related protein CARD10 was previously reported to contribute to other epithelial carcinomas [21][22][23][24], we also analysed its expression in PCa. Patients with high CARD14 expression were found to have a significantly lower Disease/progression-free survival compared with patients with low CARD14 expression ( Figure 1A). In comparison, expression of MALT1, BCL10, and CARD10 did not correlate with disease progression ( Figure 1A). In addition, expression of CARD14 is higher in tumour tissue as opposed to (paired) healthy tissue ( Figure 1B,C). Overexpression of CARD14 was also significantly associated with lymph node metastasis ( Figure 1D), while CARD14 expression was found to be higher in metastatic PCa tumours compared with primary PCa tumours ( Figure 1E). In agreement with this, TCGA analysis revealed that CARD14 levels also correlate with cancer recurrence in PCa patients ( Figure 1F). Finally, high CARD14 expression was also found to correlate with higher proliferation in tumour tissue ( Figure 1G). Together, these data show that high CARD14 expression is associated with aggressive PCa in human patients.

CARD14 Expression Is Essential for PCa Cell Survival
To further verify the in silico findings, we analysed expression of CARD14 and CARD10 proteins in three different PCa cell lines. While CARD10 was similarly expressed in LNCaP, PC3, and Du145 cells, CARD14 expression was much higher in LNCaP compared with Du145 and PC3 ( Figure 2A). As Du145 and PC3 are known to be

CARD14 Expression Is Essential for PCa Cell Survival
To further verify the in silico findings, we analysed expression of CARD14 and CARD10 proteins in three different PCa cell lines. While CARD10 was similarly expressed in LNCaP, PC3, and Du145 cells, CARD14 expression was much higher in LNCaP compared with Du145 and PC3 ( Figure 2A). As Du145 and PC3 are known to be more aggressive than LNCaP, the CARD14 expression data in cell lines do not directly correlate with the above mentioned human PCa patient data, showing that higher CARD14 expression correlates with aggressive cancer (Figure 1). The more aggressive nature of Du145 and PC3 most likely reflects other important factors such as their androgen-independence. To better understand the specific role of high CARD14 expression, we next silenced CARD14 expression in LNCaP cells using siRNA and monitored cell survival over a 3-day period by analysing the uptake of the fluorescent dye SYTOX green, which can only enter dead cells with a permeabilised cell membrane. Survival of LNCaP cells was severely reduced in the absence of CARD14 ( Figure 2B). These results suggest that CARD14-induced signalling contributes to PCa cell survival and tumorigenesis.
Biomedicines 2022, 10, x FOR PEER REVIEW 7 of 16 we next silenced CARD14 expression in LNCaP cells using siRNA and monitored cell survival over a 3-day period by analysing the uptake of the fluorescent dye SYTOX green, which can only enter dead cells with a permeabilised cell membrane. Survival of LNCaP cells was severely reduced in the absence of CARD14 ( Figure 2B). These results suggest that CARD14-induced signalling contributes to PCa cell survival and tumorigenesis.

CARD14 Expression Correlates with DNA Repair Gene Enrichment
To gain further insights into the biological processes regulated by CARD14 signalling in PCa, we performed gene ontology (GO) analysis on genes that were significantly correlated with CARD14 expression in the TCGA PRAD gene expression data. This analysis indicated that DNA repair genes were most enriched in PCa patients with high CARD14 expression ( Figure 3A). However, although additional gene set enrichment analysis (GSEA) also showed that DNA repair genes are enriched in PCa patients with high CARD14 expression, the difference was not found to be statistically significant ( Figure 3B). Therefore, we further investigated the relationship between CARD14 expression and specific DNA damage response (DDR) pathways in more detail using the TCGA PRAD gene expression data. Several DDR pathways including Fanconi anemia, base excision repair, translesional synthesis, damage sensor, homologous recombination, mismatch repair, and direct repair were found to be positively correlated with CARD14 expression ( Figure 3C). While the biological role of DDR pathway activation is to suppress tumorigenesis by maintaining genome stability, high expression of DNA repair pathway machinery genes is known to contribute to tumour resistance to radiotherapy [37]. In agreement with this, we observed that in PCa patients who received radiotherapy, CARD14 expression was higher in patients with disease progression when compared with patients who do not show disease progression ( Figure 3D).
It has been recently reported that expression of two hyperactivating CARD14 mutants identified in Pityriasis rubra pilaris patients did not induce DNA damage or increase rates of homologous recombination upon expression in U2OS human bone osteosarcoma epithelial cells, but increased the incidence of double strand breaks (DSB) during prolonged replication stress and promoted break-induced replication [38]. The latter

CARD14 Expression Correlates with DNA Repair Gene Enrichment
To gain further insights into the biological processes regulated by CARD14 signalling in PCa, we performed gene ontology (GO) analysis on genes that were significantly correlated with CARD14 expression in the TCGA PRAD gene expression data. This analysis indicated that DNA repair genes were most enriched in PCa patients with high CARD14 expression ( Figure 3A). However, although additional gene set enrichment analysis (GSEA) also showed that DNA repair genes are enriched in PCa patients with high CARD14 expression, the difference was not found to be statistically significant ( Figure 3B). Therefore, we further investigated the relationship between CARD14 expression and specific DNA damage response (DDR) pathways in more detail using the TCGA PRAD gene expression data. Several DDR pathways including Fanconi anemia, base excision repair, translesional synthesis, damage sensor, homologous recombination, mismatch repair, and direct repair were found to be positively correlated with CARD14 expression ( Figure 3C). While the biological role of DDR pathway activation is to suppress tumorigenesis by maintaining genome stability, high expression of DNA repair pathway machinery genes is known to contribute to tumour resistance to radiotherapy [37]. In agreement with this, we observed that in PCa patients who received radiotherapy, CARD14 expression was higher in patients with disease progression when compared with patients who do not show disease progression ( Figure 3D). 3E-G). However, because the correlation is small (less than 0.25) albeit statistically significant ( Figure 3E,F), the biological relevance of these data remains uncertain. Additional biological assays will therefore be needed in the future to support a possible link with genome instability.

PCa Cells Show Constitutive MALT1 Proteolytic Activity, but PCa Cell Survival Only Requires MALT1 Scaffold Activity
Activation of CARD14 nucleates the formation of a CBM (CARD14::BCL10::MALT1) signalling complex that is essential for propagation of downstream signalling ( Figure 4A) [6,7]. Importantly, akin to CARD14 silencing, BCL10 and MALT1 knockdown also re- It has been recently reported that expression of two hyperactivating CARD14 mutants identified in Pityriasis rubra pilaris patients did not induce DNA damage or increase rates of homologous recombination upon expression in U2OS human bone osteosarcoma epithelial cells, but increased the incidence of double strand breaks (DSB) during prolonged replication stress and promoted break-induced replication [38]. The latter pathway repairs one-ended DSB and is associated with elevated levels of chromosomal rearrangements known to contribute to cancer development [39]. We also found that high CARD14 expression in PCa patients somewhat correlates with a higher fraction genome altered and microsatellite instability, but not with aneuploidy score (Figure 3E-G). However, because the correlation is small (less than 0.25) albeit statistically significant ( Figure 3E,F), the biological relevance of these data remains uncertain. Additional biological assays will therefore be needed in the future to support a possible link with genome instability.

PCa Cells Show Constitutive MALT1 Proteolytic Activity, but PCa Cell Survival Only Requires MALT1 Scaffold Activity
Activation of CARD14 nucleates the formation of a CBM (CARD14::BCL10::MALT1) signalling complex that is essential for propagation of downstream signalling ( Figure 4A) [6,7]. Importantly, akin to CARD14 silencing, BCL10 and MALT1 knockdown also reduced survival of LNCaP cells ( Figure 4B). In contrast, MALT1 knockdown had no effect on PC3 and DU145 cell survival, presumably reflecting the more aggressive nature of these cells due to their androgen independence ( Figure 4C). The essential role for CARD14, BCL10, and MALT1 in LNCaP cell survival indicates the importance of CARD14-induced CBM formation, which is in agreement with the recently reported role for MALT1 in regulating proliferation and migration/invasion of PCa cells [19,20].
In addition to its scaffold function, CBM formation is known to unlock proteolytic activity of MALT1, which leads to cleavage of several substrates to fine-tune downstream signalling and gene expression ( Figure 4A) [40]. We therefore investigated MALT1 proteolytic activity at the molecular level by analysing cleavage of the known MALT1 substrates CYLD and A20 via immunoblotting. Both substrates were constitutively cleaved in LNCaP cells ( Figure 4D). Interestingly, although MALT1 knockdown in PC3 and DU145 cells had no effect on cell survival ( Figure 4C), DU145 and, to a lesser extent, PC3 cells also showed constitutive MALT1 proteolytic activity ( Figure 4D). Since DU145 and PC3 show almost no CARD14 expression, activation of MALT1 proteolytic activity in these cells is possibly mediated by CARD10, which is strongly expressed in these cell lines (Figure 2A). Constitutive cleavage of MALT1 substrates in PCa cells was inhibited upon treatment with the selective small molecule MALT1 protease inhibitor MLT-827 ( Figure 4D). However, in contrast to the strong effect of MALT1 silencing on LNCaP cell survival ( Figure 4B), pharmacological inhibition of MALT1 catalytic activity with MLT-827 treatment had no effect ( Figure 4E), suggesting that the scaffold function of MALT1 plays a primary role in regulating survival of LNCaP cells.

Proteolytic Activity of MALT1 Does Not Contribute to Constitutive NF-κB/JNK/Akt Signalling in PCa Cells, but Promotes Cancer-Associated Gene Expression
Formation of the CARD14-BCL10-MALT1 complex induces activation of NF-κB and the MAP kinases p38 and JNK, which in turn induces the expression of several proinflammatory mediators ( Figure 4A) [6]. NF-κB and JNK signalling have been shown to promote survival, proliferation, and invasion of PCa [5,41]. Therefore, we investigated the activation status of these pathways in PCa cells (LNCaP, PC3, and DU145) by analyzing IκBα and c-Jun phosphorylation, respectively. All three PCa cell lines showed constitutive IκBα and c-Jun phosphorylation ( Figure 5A), indicative of constitutive CBM activity. We also observed constitutive Akt phosphorylation, which can mediate a number of downstream effects including mTOR activation, another key pathway in PCa [42]. Inhibition of MALT1 proteolytic activity by treatment of PCa cells with the MLT-827 had no effect on IκBα, c-Jun, or Akt phosphorylation again underscoring the dominant role of the scaffold function of MALT1 in CBM-mediated NF-κB and JNK signalling in PCa cells. Interestingly, treatment with a broad spectrum inhibitor of protein kinase C (PKC) enzymes, which are known upstream activators of CARD14 family proteins [43], not only failed to inhibit NF-κB/JNK/Akt phosphorylation, but also MALT1-mediated CYLD and A20 cleavage ( Figure 5A), suggesting that constitutive CBM activation in PCa cells is mediated by a PKC-independent mechanism.  While the scaffold function of MALT1 is required for NF-κB activation and gene expression, MALT1 proteolytic activity mainly controls gene expression at the posttranscriptional level by regulating mRNA stability of specific genes, including IL-6, in an NF-κB-independent manner ( Figure 4A) [40]. IL-6 is a cytokine that plays an important role in cancer progression [44], and treatments that inhibit IL-6 signalling are expected to have a beneficial effect in lymphoid malignancies, as well as in patients with solid tumours, including PCa. Therefore, we measured secretion of IL-6 by PCa cell lines in the presence or absence of MALT1 proteolytic activity. DU145 and PC3 cells were found to secrete high levels of IL-6, while LNCaP cells produced only minor amounts of this cytokine ( Figure 5B). IL-6 secretion was significantly inhibited in DU145 and PC3 cells treated with MLT-827, suggesting that the proteolytic activity of MALT1 regulates IL-6 expression in these cells ( Figure 5B). Tsui and colleagues previously suggested that knockdown of MALT1 in PC3 cells results in lower expression of IL-6 which correlated with reduced tumorigenesis in a xenograft mouse model [20]. Therefore, we further focused on PC3 cells to screen for cancer-related proteins that are regulated by the proteolytic activity of MALT1. To this end, we used MALT1 knockout PC3 cells that were reconstituted with either wild type or catalytically inactive MALT1. MALT1 proteolytic function in reconstituted cells was verified by analysing CYLD cleavage ( Figure 5C). While no CYLD cleavage could be observed in MALT1-deficient PC3 cells or cells reconstituted with the catalytically inactive mutant, wild type MALT1 restored CYLD cleavage in PC3 cells. We next used Human Oncology Array to compare 84 cancer-related proteins in PC3 cells expressing either wild type or catalytically inactive MALT1 ( Figure 5C). This revealed that expression of several cancerassociated proteins, including GM-CSF, CCL20 and VCAM1, was strongly dependent on MALT1 catalytic activity in PC3 cells. Collectively, our results demonstrate that although CARD14-induced proteolytic activity of MALT1 does not regulate survival of LNCaP cells, constitutive activation of MALT1 contributes to the production of several cancer-related proteins, highlighting the potential of pharmacological inhibition of MALT1 as a novel therapeutic strategy for PCa.

Discussion
In the present study, we reported a novel functional role for CARD14 signaling in driving PCa cell survival. We showed that high expression of CARD14 correlates with

Discussion
In the present study, we reported a novel functional role for CARD14 signaling in driving PCa cell survival. We showed that high expression of CARD14 correlates with reduced patient survival, invasiveness, and recurrence in human PCa patients. Survival of LNCaP PCa cells was shown to be dependent on CARD14 expression and MALT1 scaffold activity. Moreover, we demonstrated constitutive MALT1 proteolytic activity in several PCa cell lines, which regulates the expression of several cancer-related genes.
Multiple cancers are driven by genetic or epigenetic alterations that dysregulate intracellular signalling pathways controlling cell proliferation and migratory capacity, cell survival, metabolism, genetic instability, and induction of angiogenesis. Anticancer drugs targeting such dysregulated signalling pathways in tumour cells are therefore of high interest, but the redundancy of pathways that control cell proliferation and survival presents a huge challenge. Moreover, many signalling pathways are shared by multiple cell types, leading to an increased risk of side effects on healthy cells. This is well illustrated by the NF-κB signalling pathway, which regulates the expression of several genes associated with the aforementioned hallmarks of cancer. However, NF-κB signalling also steers normal immune responses in multiple cell types; thus, the clinical development of NF-κB inhibitors as anti-cancer drugs has failed because of severe side effects. Overcoming these challenges requires the identification and detailed characterisation of more cell typespecific signalling molecules and mechanisms. In this context, our observation that high CARD14 expression in PCa patients correlates with more aggressive PCa, together with the finding that CARD14 expression is essential for survival of LNCaP PCa cells, reveal CARD14 as a novel anti-cancer target in PCa. Importantly, the rather specific expression of CARD14 in a subset of epithelial cells, together with the absence of a major phenotype in CARD14-deficient mice [46], suggests that side effects of specific CARD14 targeting may be quite limited. Until recently, intracellular adapter proteins such as CARD14 have long been considered undruggable, due to the absence of any enzymatic activity or active site. However in recent years, novel therapeutics against non-enzymatic targets has become more achievable with the development of PROteolysis TArgeting Chimera (PROTAC) inhibitors, bifunctional compounds capable of inducing target protein ubiquitination and proteasomal degradation [47]. Several PROTAC drugs are now under clinical development in cancer, and the field is rapidly growing. The future development of CARD14 PROTACs and the proof-of-concept of their use as therapeutics against certain prostate cancer types may have a high impact in the cancer field. CARD14 signalling is mediated by MALT1, which has dual activities by acting as a scaffold which activates downstream NF-κB signalling, as well as by exerting proteolytic activity that regulates specific gene expression at the posttranscriptional level. Our data revealed that, similar to CARD14, MALT1 expression is also essential for LNCaP cell survival. Therefore, MALT1 targeting PROTACs may have potential for PCa treatment, although its ubiquitous expression may pose a significant risk for side effects. Alternatively, inhibition of CARD14 signalling can also be achieved by targeting MALT1 proteolytic activity using small molecule inhibitors. Importantly, although we showed that MALT1 enzymatic activity is not required for PCa cell survival, it does contribute to PCa tumour cell expression of several proteins that play key roles in PCa development and progression. Small molecule MALT1 inhibitors have already shown promising results in a number of preclinical cancer models for ABC-DLBCL, in which MALT1 is also constitutively activated [48]. However, several studies, including work from our group, have recently shown that long-term inhibition of MALT1 proteolytic activity is associated with systemic autoimmunity due to defective regulatory T cell function and increased effector T cell function, raising concerns about the safety of MALT1 inhibition [49][50][51][52]. Therefore, because of its more specific expression in certain epithelial cells, direct targeting of CARD14 (instead of MALT1) may be a more preferred approach in PCa treatment.
The mechanism of CBM complex activation in PCa cells remains to be elucidated. Activation of CARD-CC family members usually requires phosphorylation by specific PKCs [43]. However, we showed that PKC inhibitors failed to block CBM activation in PCa cells. It is possible that high expression of CARD14 in LNCaP cells (or possibly CARD10 in PC3 and DU145) is sufficient to overcome the autoinhibition of these proteins. In line with this, we previously showed that overexpression of CARD14 or CARD10, but not CARD11, is sufficient to initiate CBM signalling in HEK293T cells [43]. Alternatively, gainof-function mutations of CARD14 are known to circumvent the need for upstream activation and initiate downstream signal transduction [6]. In this regard, it will be interesting to investigate the activity of CARD14 mutants that has been described in lethal castrationresistant PCa [26].
An important limitation of our current study is that the results presented here were obtained with PCa cell lines, failing to reproduce the complex tumour microenvironment. However, others have previously shown that xenograft tumour development was significantly attenuated when tumours were derived from PCa cells lacking MALT1 expression in a mouse model, supporting the role for CBM signalling in tumour formation in a complex microenvironment [19,20]. Further in vivo studies are expected to provide a better mechanistic understanding of the CARD14-BCL10-MALT1 axis in PCa and possibly even other carcinomas, eventually opening new therapeutic opportunities for targeting CARD14mediated signalling in PCa and beyond.
Supplementary Materials: The following supporting information can be downloaded at: https:// www.mdpi.com/article/10.3390/biomedicines10082008/s1, Table S1. DNA damage response (DDR) gene list assembled from an online catalogue of DDR genes from recently published resources. Data Availability Statement: Publicly available datasets were analyzed in this study, as referred to in Materials and Methods section. Other data available on request.