The Anti-Inflammatory Effects of Iberin on TNF-α-Stimulated Human Oral Epithelial Cells: In Vitro Research

Iberin is a bioactive chemical found in cruciferous plants that has been demonstrated to have anticancer properties. However, there have been no reports on its effects on periodontal resident cells, and many questions remain unanswered. The aim of this study was to examine whether iberin had anti-inflammatory effects on human oral epithelial cells, including influences on signal transduction pathway activation in TNF-α-στιμυλατεd χελλσ. Iberin inhibited the production of interleukin (IL)-6 and C-X-C motif chemokine ligand 10 (CXCL10), as well as the expression of vascular cell adhesion molecule (VCAM)-1, inducible nitric oxide synthase (iNOS), and cyclooxygenase (COX)-2 in tumor necrosis factor (TNF)-α-stimulated TR146 cells, a human oral epithelial cell line. Moreover, iberin administration increased the expression of antioxidant signaling pathways, such as Heme Oxygenase (HO)-1 and NAD(P)H quinone dehydrogenase 1 (NQO1). Furthermore, we found that iberin could inhibit the activation of the nuclear factor (NF)-κB, signal transducer and activator of transcription (STAT)3, and p70S6 kinase (p70S6K)-S6 ribosomal protein (S6) pathways in TNF-α-stimulated TR146 cells. In conclusion, iberin reduced inflammatory mediator expression in human oral epithelial cells by preventing the activation of particular signal transduction pathways.


Introduction
Periodontitis is a chronic inflammatory disorder in periodontal tissues caused by periodontopathic bacteria such as Porphyromonas gingivalis [1]. An overactive immune system to microorganisms causes periodontal tissue destruction [1,2]. At the moment, periodontal lesions are treated with antibiotics that are supplied locally in the conventional treatment of periodontal disease. Since the problem of resistant bacteria has been raised [3], new anti-inflammatory compounds are expected to be identified.
Isothiocyanate is the generic name for substances with the chemical group -N=C=S, obtained by replacing the oxygen in the isocynate group with sulfur. Most natural isothiocyanates of plant origin are produced by enzymatic conversion of metabolites called glucosinolates [4]. Isothiocyanates are nutrients found mainly in cruciferous vegetables, and have been reported to have bioactive effects, including anticancer [5,6] and antiinflammatory effects [6]. Iberin is an isothiocyanate that can be found in green and yellow vegetables, such as cruciferous plants. We selected iberin in this research because few reports have investigated the bioactive effects of iberin compared to the well-known isothiocianate, such as sulforaphane. Additionally, it has been reported that iberin is considered as a special member of the isothiocyanate family with little cytotoxicity to normal cells [7]. Several studies on the bioactive effects of iberin have been published in recent years [8,9]. Gong et al. reported that iberin treatment inhibits cell proliferation and induces cell apoptosis in oval cancer cell lines [8]. Pocasap et al.

also demonstrated that iberin increases
Biomedicines 2022, 10, 3155 2 of 12 intracellular reactive oxygen species and tubulin depolymerization to have anticancer activity in hepatocellular carcinoma cell HepG2 cells [9]. However, there have been few studies looking at the anti-inflammatory properties of iberin. We also could not find any studies that have focused on the effects of iberin on periodontal resident cells.
We focused on the anti-inflammatory effects of iberin on human oral epithelial cell lines in this study. We specifically examined how iberin affected the expression of vascular cell adhesion molecule (VCAM)-1, inducible nitric oxide synthase (iNOS), and cyclooxygenase (COX-2), as well as the production of interleukin (IL)-6 and C-X-C motif chemokine ligand (CXCL)10 in tumor necrosis factor (TNF)-α-stimulated human oral epithelial cells. Iberin's effects on the signal transducer and activator of transcription 3 (STAT3), nuclear factor (NF)-κB, and p70S6 kinase (p70S6K)-S6 ribosomal protein (S6) activation were also examined. Further, we investigated the effects of iberin on antioxidant expression, such as Heme Oxygenase (HO)-1 and NAD(P)H quinone dehydrogenase 1 (NQO1).

Cytotoxicity Assay
Cell viability was measured using Cell Count Reagent SF (Nakarai Tesque). TR146 cells were seeded in 96-well plates and incubated for 48 h. After 48 h, the media were discarded and 90 µL of Ham's F12 medium with varying concentrations of iberin (Cayman Chemical, Ann Arbor, MI, USA) was added, and the cells were cultured for another 24 h. Then, we added 10 µL Cell Count Reagent SF and incubated the cells for 2 h before measuring the absorbance at 450 nm with a microplate reader.

IL-6 and CXCL10 Production in TR146 Cells
TR146 cells were stimulated for 24 h with TNF-α (100 ng/mL: Peprotech, Rocky Hill, NJ, USA) with or without iberin (1.875, 3.75, 7.5, or 15 µM). The concentration of IL-6 and CXCL10 in cell culture supernatant of TR146 cells were determined using DuoSet ELISA Development Systems (R&D systems, Minneapolis, MN, USA) in according to the manufacturer's instructions.

Statistical Analysis
One-way analysis of variance (ANOVA) was used to determine statistical significance, followed by a post hoc Tukey-Kramer test. p values less than 0.05 were regarded as significant.

Statistical Analysis.
One-way analysis of variance (ANOVA) was used to determine statistical significance, followed by a post hoc Tukey-Kramer test. p values less than 0.05 were regarded as significant.

Effects of Iberin on Cell Viability of TR146 Cells.
First, we investigated at how iberin affected the viability of TR146 cells. Figure 1 demonstrates that iberin (1.875-15 μM) had no effect on the viability of TR146 cells. As a result, iberin (1.875-15 μM) was used in this experiment.

Figure 2.
Effects of iberin on TNF-α-induced production of CXCL10 and IL-6. TR146 cells were grown for 24 h with TNF-α (100 ng/mL) with or without iberin (1.875-15 μM). CXCL10 and IL-6 levels in the supernatant were measured using the ELISA kits indicated in the Materials and Methods section. The data are presented as the mean SD of three independent experiments. * = p < 0.05, significantly different from TNF-α-stimulated TR146 cells without iberin.

Iberin Inhibits VCAM-1, iNOS, and COX-2 Expression in TNF-α-Stimulated TR146 Cells
Next, we wanted to know how iberin affects the expression of additional inflammatory mediators besides cytokines. We focused on VCAM-1, iNOS, and COX-2 expression since we knew they played a role in periodontal disease development [12][13][14].  Effects of iberin on TNF-α-induced production of CXCL10 and IL-6. TR146 cells were grown for 24 h with TNF-α (100 ng/mL) with or without iberin (1.875-15 µM). CXCL10 and IL-6 levels in the supernatant were measured using the ELISA kits indicated in the Materials and Methods section. The data are presented as the mean SD of three independent experiments. * = p < 0.05, significantly different from TNF-α-stimulated TR146 cells without iberin.

Effects of Iberin on HO-1 and NQO1 Expression in TR146 Cells
Reactive oxygen species and the resulting oxidative stress are significant in the onset and progression of periodontal disease [15]. As a result, it is critical to enhance the expression of the antioxidants (HO-1 and NQO1) in periodontal lesions. Thus, we looked into the effects of iberin on HO-1 and NQO1 expression. Figure 4 indicates that iberin treatment significantly increased the expression of HO-1 and NQO1 in TR146 cells compared to TR146 cells that were not treated with iberin.

Effects of Iberin on HO-1 and NQO1 Expression in TR146 Cells
Reactive oxygen species and the resulting oxidative stress are significant in the onset and progression of periodontal disease [15]. As a result, it is critical to enhance the expression of the antioxidants (HO-1 and NQO1) in periodontal lesions. Thus, we looked into the effects of iberin on HO-1 and NQO1 expression. Figure 4 indicates that iberin treatment significantly increased the expression of HO-1 and NQO1 in TR146 cells compared to TR146 cells that were not treated with iberin.

Discussion
Periodontitis is a chronic inflammatory disease caused by periodontopathogenic bacteria [1]. Local administration of antimicrobial drugs has been used for the treatment of

Discussion
Periodontitis is a chronic inflammatory disease caused by periodontopathogenic bacteria [1]. Local administration of antimicrobial drugs has been used for the treatment of periodontitis, but due to the problem of bacterial resistance, the discovery of new bioactive compounds with anti-inflammatory action has lately been requested [3]. We concentrated on the bioactive compounds found in cruciferous plants, a popular food worldwide. This is because we felt that all people could utilize it securely.
We were able to find a few articles that investigated the anti-cancer properties of iberin [8,9]. However, there have been limited studies on the anti-inflammatory properties of iberin. Shibata et al. discovered that iberin had an anti-inflammatory effect on toll-like receptor (TLR) ligand-stimulated RAW 264.7 cells, a macrophage cell line. In lipopolysaccharide (LPS) or Pam3CSK4-treated RAW 264.7 cells, iberin inhibited iNOS, COX-2, TNF-α, and IL-1α expression, as well as NF-κB activation. They also reported that because iberin inhibited TLR dimerization, it did not reduce the NF-κB activity generated by phorbol 12-myristate 13-acetate, a TLR-independent proinflammatory ligand [17]. Although we did not use TLR ligands in this investigation, iberin decreased the expression of inflammatory mediators. We need to conduct further research to figure out how iberin decreased TNF-α-induced inflammatory mediator production.
Many studies have revealed that isothiocyanates, such as sulforaphane, activate NF-E2-related factor(Nrf)2/HO-1 signaling [18][19][20][21]. However, there have been few reports that iberin stimulates antioxidants such as HO-1 and NQO1. Ernst et al. showed that iberin administration raised nuclear Nrf2 levels and HO-1 expression in the murine fibroblast cell line NIH3T3 [22]. We believe this is the second article showing that iberin can produce antioxidants.
In TNF-α-stimulated TR146 cells, iberin inhibited the activation of NF-κB, STAT3, and p70S6K-S6. Our prior research also found that 6-MSITC, an isothiocyanate containing wasabi, inhibited the NF-κB, STAT3, and p70S6K-S6 pathways [16]. Shibata et al. reported NF-κB activation was inhibited by iberin treatment in LPS-stimulated macrophages [17]. Recently, it was reported that STAT3 phosphorylation in immortalized bone marrow-derived macrophages stimulated by LPS is inhibited by sulforaphane, a type of isothiocyanate [23]. It has also been reported that sulforaphane inhibits p70S6K phosphorylation in plateletderived growth factor-treated vascular smooth muscle cells [24]. Although further studies are needed, based on our findings and those of others, suppression of the NF-κB, STAT3, and p70S6K-S6 pathways may be a common property of isothiocyanates.
Recently, it has been reported that the bioactive effects of isothiocyanates occur via bitter taste receptors (T2R). Qin et al. reported that N-C=S-containing compounds including isothiocyanates are detected via T2R38 receptors in Caco-2 cells [25]. It has also been shown that T2R is expressed in oral epithelial cells and is involved in the innate immune response. Previous reports showed that T2R14 is expressed in gingival epithelial cells (GECs) and interacts with competence-stimulating peptides (CSPs) secreted by the Storeptococcus mutans [26,27]. T2R agonists induce TNF-α, IL-6, IL-8 and beta-defensin2 production in GECs [27,28]. These reports indicate that in the oral cavity, taste receptors are actively involved in the innate response by recognizing bacterial components [29]. In addition, isothiocyanates such as iberin may bind to bitter taste receptors in the oral cavity and exert bioactive effects. We believe that more research on the regulation of inflammation in the oral cavity with a focus on bitter taste receptors is needed.

Conclusions
In this paper, we have shown that iberin can decrease the production of inflammatory mediators such as IL-6, CXCL10, VCAM-1, iNOS, and COX-2 while increasing antioxidants in TNF-α-treated human oral epithelial cells. Iberin administration to the periodontal legions may therefore be utilized to treat periodontitis. However, in order to use iberin for the treatment of periodontitis in humans, it is necessary to study its effects on periodontal tissue constituent cells other than human oral epithelial cells. It is also important to examine the effects of iberin on animal models of periodontitis.