Dipsticks with Reﬂectometric Readout of an NIR Dye for Determination of Biogenic Amines

: Electrospun nanoﬁbers (ENFs) are remarkable analytical tools for quantitative analysis since they are inexpensive, easily produced in uniform homogenous mats, and provide a high surface area-to-volume ratio. Taking advantage of these characteristics, a near-infrared (NIR)-dye was doped as chemosensor into ENFs of about 500 nm in diameter electrospun into 50 µ m thick mats on indium tin oxide (ITO) supports. The mats were made of cellulose acetate (CA) and used as a sensor layer on optical dipsticks for the determination of biogenic amines (BAs) in food. The ENFs contained the chromogenic amine-reactive chameleon dye S0378 which is green and turns blue upon formation of a dye-BA conjugate. This S N 1-reaction of the S0378 dye with various BAs was monitored by reﬂectance measurements at 635 nm where the intrinsic absorption of biological material is low. The di ﬀ erence of the reﬂectance before and after the reaction is proportional to BA levels from 0.04–1 mM. The LODs are in the range from 0.03–0.09 mM, concentrations that can induce food poisoning but are not recognized by the human nose. The calibration plots of histamine, putrescine, spermidine, and tyramine are very similar and suggesting the use of the dipsticks to monitor the total sample BA content. Furthermore, the dipsticks are selective to primary amines (both mono- and diamines) and show low interference towards most nucleophiles. A minute interference of proteins in real samples can be overcome by appropriate sample pretreatment. Hence, the ageing of seafood samples could be monitored via their total BA content which rose up to 21.7 ± 3.2 µ mol / g over six days of storage. This demonstrates that optically doped NFs represent viable sensor and transducer materials for food analysis with dipsticks.


Introduction
Biogenic amines (BAs) are important compounds that can determine the quality of food [1]. High levels of amines in food are produced by bacterial decarboxylation of amino acids and have been recognized as an important reason for seafood intoxication [2]. Hence, the determination of the concentration of BAs in fresh food is in high need to determine its freshness status. Papageorgiou and co-workers gave a review on food and beverage products that should be regularly tested since they contain BAs or may develop certain contents over (storage) time [3]. This raises interest in research on rapid and inexpensive optical detection methods and tools like dipsticks to determine BAs in food, not only for individual concentration levels but also as a sum parameter. According to the European Food Safety Authority (EFSA), the U.S. Food and Drug Administration (FDA) as well as the the detection wavelength and a detector (which can be a low-cost digital camera). Evaluation of data can then be carried out by free available software. Hence, a complete sensor (array) can be obtained for a few hundred US-$ or less including detection equipment. BA sensing was carried out reflectometrically by a digital camera and data were analyzed using the color space of the International Commission on Illumination (CIE) system [16]. These sensors were applied for quantitation of various amines and ammonia produced during food ageing in food packages. Fish freshness was monitored reflectometrically using a colorimetric sensor of creatine in fish, which is an indicator for the fish ageing [30].
In order to create dipsticks for food analysis with improved features, the following novel features were implemented: (a) a sensor layer containing only one dye instead of two to simplify the dipsticks compared to earlier research [29]; (b) using reflectometry for a one-step detection (instead of a two-step detection process as formerly required with digital photography evaluation [19]); and (c) using an NIR chromogenic dye to reduce the effects of self-absorption and scatter upon measuring in real samples.
Therefore, for the first time, an NIR dye reactive to BAs was embedded into a mat of electrospun nanofibers to form reflectometric dipstick sensors. The electrospun nanofibers made from cellulose acetate (CA) are prepared by a simple standard electrospinning procedure and contain the S0378 cyanine dye which absorbs at 800 nm. CA is stable over a wide range of pH values and contains many hydroxyl (OH) and ester groups which render it highly hydrophilic. This allows easy access of polar analytes like BAs to the S0378 chemosensor embedded inside the NFs. Additionally, CA can be easily electrospun into layers of several tens of µm, as common in optical chemical sensors. Primary amines react with the dye by an S N 1 nucleophilic substitution mechanism which is accompanied by a color change from green to blue. Hence, the concentration of BAs can be determined based on reflectance detection which is a simple and fast readout. The equal response towards monoamines and diamines makes the dipstick an ideal tool for determination of the total amine content (TAC) in real samples which was demonstrated by monitoring the ageing of shrimp samples over time.

Materials
S0378 was from FEW Chemicals (www.few.de). The buffer N-cyclohexyl-2-amino ethanesulfonic acid (CHES) was from Roth (www.carlroth.de). Spermidine (SPR), putrescine (PUT), and histamine (HIS) were purchased from Sigma-Aldrich (www.sigmaaldrich.com), all as hydrochloride salts. Tyramine (TYR), cysteine (CYS), triethylamine (TEA) and dimethylamine, each as free base, were from Sigma, Mann research laboratories, Merck and Fluka, respectively. Cellulose acetate (CA) (Mw 30,000 Da, 39.8 wt% acetyl content) and human serum albumin (HSA) and all organic solvents (methanol, acetic acid and acetone) were obtained from Sigma-Aldrich. All chemicals were of analytical grade. Indium tin oxide (ITO) coated on polyethylene terephthalate (PET) with a surface resistivity 60 Ω/sq and 127 µm thickness was purchased from Sigma-Aldrich. Stock solutions of BA (10.0 mM) were prepared in CHES buffer (pH 9.7). Standard solutions of the BAs were freshly prepared by diluting stock solutions with CHES buffer. CHES buffer (5.00 mM) was prepared by dissolving of solid CHES (0.1036 g) in 100.0 mL of deionized water. The pH of CHES was adjusted with sodium hydroxide solution (1.00 M, from Merck (www.merckgroup.com)).

Apparatus
The electrospinning was performed using a home-built electrospinning setup (see Figure S1) with an iseg T1 CP300p high voltage power supply (www.iseg-hv.com) and a syringe pump (Legato 180 from KD Scientific, www.kdscientific.com). Fiber mat thickness, fiber diameter, and images for pore size determination were obtained by a scanning electron microscope (Zeiss/LEO 1530, City, Germany) at 5.0 kV. Samples for SEM were cut with a pair of scissors and sputtered with gold for 30 s (≈7 nm layer thickness). For the study of fiber morphology, fibers were randomly selected from different regions of the SEM image. The fiber diameters within the field of view were quantified by ImageJ analysis software (ImageJ, National Institutes of Health). Within the same SEM image, pore regions were randomly analyzed. Pores surrounded by fiber networks were drawn manually and analyzed by ImageJ, where Feret's diameter is reported as a pore size. A fraction of the sample prepared for pore size determination was cut and mounted on a 90 • tilted high-profile SEM stub for determining the fiber mat thickness. Within the same sample, different locations (at least three) were imaged and analyzed for mat thickness. Image analysis was performed using the SEM software (Zeiss).
Reflectance spectra were acquired with an AB2 luminescence spectrometer with a 150 W xenon light source. The reference for reflectance spectra was MgSO 4 . The light was guided to the dipstick by a y-shaped bifurcated optical fiber, a fiber holder, and the samples were illuminated under an average illumination angle of 33 • . The inner central fiber bundle at the tip of the bifurcated optical fiber collects the reflected light from the dipstick and guides it back into the spectrometer. Two wavelengths (650 and 635 nm) were used for illumination and the dipsticks were placed in home-made black plastic holders below the tip of the optical fiber to ensure a flat and reproducible positioning of the dipsticks with respect to the incident light beam. The distance between the dipstick and the tip of the optical fiber is small (3 mm) to increase the collected reflected light and improve the sensitivity. The measurements were done in synchronous mode of the spectrometer i.e., the emission was measured at the same wavelength as the excitation. The band passes are 4 nm for both, excitation and emission monochromators.
The absorbance spectra were acquired on a Varian Cary 50 Bio photometer in quartz cells. The emission spectra were obtained using an AB2 luminescence spectrometer equipped with a cell holder with 90 • arrangement of excitation and emission beam and no fiber optic. The excitation wavelength was 600 nm and the bandpasses for excitation and emission were 4 nm and 8 nm, respectively.

Electrospinning of S0378-CA Fiber Mats
The polymer CA (0.720 g) and S0378 (30.00 mg) were dissolved in a mixture of 3.00 mL of acetic acid and 1.00 mL of acetone. Then, this spinning dope was first sonicated at room temperature for 30 min, then at 40 • C for another 30 min and finally stirred for about 1 h until the mixture was completely homogeneous. The spinning dope was protected from light by Al-foil while stirring, storage, and electrospinning. The S0378-CA electrospun nanofibers were fabricated using an electrospinning setup with the following parameters: spinning dope in plastic syringe 5 mL (covered with aluminum foil); voltage 17 kV; flow rate 0.002 mL/min; tip-to-collector distance, 11 cm, 15 min spinning time (see Table S1). An ITO sheet (size 7 × 5 cm) was used as a supporting material. Electrospun fiber mats deposited on ITO should be stored in a desiccator.

Preparation of Dipsticks and BA Determination
The dipsticks with S0378-CA nanofibers (Ø = 8 mm) were cut from the ITO sheets with a toggle press from BERG & SCHMID (www.bergundschmid.de) and placed on black, solvent-resistant plastic positioners. After that, 10.00 µL of either BA (in CHES buffer, pH 9.7) or an extract from the real sample were added onto the spots. The color change of the dipsticks took place at 130 • C for 30 min in an oven. The reflectance was measured two times for each dipstick before and after the reaction with BAs.

Preparation of Real Samples
Histamine concentration in real samples was determined using methanolic extraction and a matrix matched calibration curve for concentration determination. The extraction follows the AOAC method 35.1.32 [31] with slight modifications. Shrimp samples were purchased from a local supermarket and stored at −80 • C. A 10.0 g portion was mixed with 50 mL of methanol in a beaker and homogenized in a blender at the highest speed for 2 min. The homogenate was transferred into a conical flask and heated at 60 • C in a water bath for 30 min. 0.19 M Carrez solution I (potassium ferrocyanide) and 1.05 M Carrez solution II (zinc acetate) were prepared in distilled water. In addition, 2 mL of each Carrez solution were added to the shrimp extract before filtration of the homogenate for protein precipitation.
The extract was filtered through a porcelain Buchner funnel with blue ribbon filter paper (Schleicher und Schüll: 589 3 , www.whatman.com) twice to yield a clear solution. Then, different volumes were taken from the sample extract to achieve a suitable dilution factor on each ageing day and histamine solution was added to reach concentrations of 0-600 µM of added histamine after dilution to 500 µL overall volume with CHES buffer (5.00 mM; pH 9.7). After that, 10.00 µL of aliquot solutions were added onto dipsticks and the reflectance of each dipstick was measured, as indicated above.

Choice of Dye
The main idea of this work was to produce the first NIR-dye-embedded electrospun nanofiber mats which act as sensor layers in dipsticks for visual and reflectometric evaluation of BAs. Upon reaction with BAs (from real samples), the embedded dye shows a color change from green (λ max abs = 800 nm) to blue (see Figures 1 and 2). The S0378 dye was chosen because of its chameleon property and the reaction with the BA (e.g., tyramine) occurs in CHES buffer at pH 9.7. This pH is required to warrant a free electron pair to be present at the amino group of the BA for a nucleophilic attack at the electrophilic carbon atom in the center of the π-electron system of the cyanine dye. Then, the electron-withdrawing chloro group is replaced by the electron-donating amino group of the BA in an S N 1 reaction. This inexpensive cyanine dye was formerly used as long wavelength-absorbing protein label [32]. Additionally, the CA fibers assist in the response of the ENF mat as they are anionic at pH 9.7 and thus can attract cationic BAs from the sample.  Furthermore, the emission maxima of S0378 at 663 nm and at 820 nm strongly decrease upon conjugation with BAs as presented in Figure S2. Hence, the determination of BAs could be done by either fluorimetry or reflectometry. As the use of a simple detection method suitable for the evaluation of dipsticks was one of the major aims of this work, reflectometry based on the color change was chosen in this work.
The reflectance spectra of S0378 dye (spun into a fiber mat of CA polymer) show an overall decrease when reacted with tyramine ( Figure 3). The sharp peaks between 400 nm and 525 nm are the characteristic peaks of the xenon excitation lamp and hence instrumental artifacts. Therefore, a much longer detection wavelength closer to the absorption maximum of S0378 is advisable for the reflectance measurements. This is further supported by the fact that the self-absorption of biological matter strongly decreases at longer wavelengths, which in turn can lead to higher reflectance. It was intended to detect food extracts, and the much higher reproducibility of the reflectance change at longer wavelengths 650 nm was chosen for method optimization and 635 nm for quantitative reflectance detection in real samples.

Choice of Nanofiber Materials, Conditions of Spinning, Reaction Temperature and Time, and Fiber Morphology
The spinning dope used for the electrospinning of the nanofibers was prepared by dissolving the S0378 dye and CA polymer in a solvent mixture of acetic acid and acetone. The electrospinning conditions, the polymer, and the solvents used resemble those used in an earlier study [29] except for dye concentration and spinning time. The concentration of the dye molecules was increased to enhance the sensitivity of the reflectance measurements. It was also tested if reducing the spinning time by half (15 min) still yields a stable fiber mat and avoids detachment of the mat off the ITO sheet. Hence, the spinning time was varied between 15 min and 30 min and the dye concentration in the spinning dope between 7.5 mg/mL and 15 mg/mL. The effects on the change of the reflectance signal after a reaction of the fiber mat with histamine are depicted in Figure 4. Here, lower dye concentrations lead to lower changes of reflectance (∆R). In all combinations of dye concentration and spinning time ∆R increases with increasing concentration of HIS. On comparing the change of ∆R at 0.8 mM of HIS with ∆R in absence of HIS, there is not too large of a difference among all the four combinations of dye concentration and reaction time. Therefore, a concentration of the dye in the spinning dope of 7.5 mg/mL and 15 min spinning time were chosen to save dye and time for preparation of the sensor mats deposited on the dipstick. Other spinning conditions did not provide a larger dynamic range in calibration plots for determination of BAs. Humidity was controlled and kept in a range from 40%-55%. No effect on fiber quality on fiber was observed, if humidity remained in that range. The reaction of the S0378-CA fiber mat and BAs on the dipsticks was allowed to develop at various temperatures after addition of histamine solution in CHES buffer (pH 9.7) to accelerate the color change. Figure 5 shows that the reaction between the dye embedded inside the electrospun nanofibers was carried out at three temperatures 70, 100, and 130 • C for 30 min. The highest increase of the reflectance is observed at 130 • C. This was expected because it was known from earlier literature [32] that the S N 1 reaction of S0378 with e.g., amino-side chains of proteins is not fast, even in solution. In order to obtain a wide detection range and a more reproducible response of the dipsticks, the reaction with BAs was carried out at 130 • C in all subsequent measurements. Figure S3 shows the response of the dipsticks after different development times (10, 20, and 30 min at 70 • C). A development time of 30 min provided the highest change of reflectance and hence better sensitivity. No effect of humidity on the in detected reflectance change was noticed, if humidity was in a 30%-65% range. Characterizations of fiber morphology with respect to fiber mat thickness, pore size, pore density and diameter of the S0378-doped nanofibers prior to their reaction with BAs were performed by scanning electron microscope (SEM) images. An example of a nanofiber mat is displayed in Figure 6. The thickness of the resulting fiber mat was determined to be 50.7 ± 8.4 µm. The pore sizes are 2.80 ± 0.15 µm as determined by the Feret diameter (example given in Figure S4) from 1330 pores. In addition, the density of pores is very high (~1.94 × 10 5 pores/mm 2 ), thus providing a great surface area for interaction with BAs (see Table S1). The average diameter of the fibers is 496 ± 318 nm (n = 82). The fibers are not round but have an overall shape of two fibers fused with one another (see Figure S5a). Hence, there is a short and a long axis to describe the fiber size (see Figure S6), and the average ratio between the diameters along the short and long fiber axis is 0.62. This also explains the large standard deviation of the fiber diameter. The surface of the fibers is not smooth but rather undulated. After the reaction with 1.0 mM of tyramine in CHES buffer, the fibers became considerably thinner with average diameters of 165 ± 124 nm (n = 82) but still retained a rough surface (see Figure S5b).

Visible Color Change of Dipsticks
The S0378-CA fiber mats on the dipsticks are greenish and turn blue upon the reaction with a primary biogenic amine (like histamine in aqueous solution of pH 9.5-10). This is due to the S N 1 nucleophilic substitution of the chlorine atom by the nitrogen atom of the BA. The blue color becomes more intense with increasing the BA concentration as displayed in Figure 7. This color change is most visible starting from a BA concentration of 0.2 mM, which nicely coincides with BA concentrations (0.3-1 mM) in foods that can induce serious health problems [19] but cannot be detected by the human nose. Reflectance detection based on the color change was chosen to determine BAs because it has a low instrumental demand and reasonable sensitivity. With an appropriate device at hand, in-field use of the dipsticks seems feasible, eventually even with less qualified personnel, at a later stage of application. Furthermore, reflectometry is the typical and well established method for evaluation of dipsticks and also the vision of the human eye is based on the perception of reflected light.

Assay Procedure for Quantitation of BAs
Nanofiber mat circles (Ø = 8 mm) were cut from the fiber mats sheets with a toggle press and placed on a positioning device made of black solvent-resistant plastic to absorb stray light. The dipsticks were illuminated by a Y-shaped bifurcated optical fiber (with red light) at 33 • illumination angle and the original reflectance of each dipstick was measured as illustrated in Figures S7 and S8. A series of BA solutions of different concentrations was prepared and added onto the dipsticks for calibration. The real sample extracts were delivered to the dipsticks as a methanolic/aqueous mixture using the standard addition method. After adding of the BA solution onto the dipstick, the liquid spread all over the dipstick area. The dipsticks were then transferred to glass slides to allow the reaction with the S0378 dye embedded in the fibers to occur in an oven at 130 • C for 30 min. Then, the reflectance of all dipsticks was measured again. The reflectance of each dipstick was measured twice before and after the reaction with BAs. This considers potential differences of the reflectance of each dipstick originating from minute differences of the thickness of the fiber mats and of mispositioning. The percentage of the reflectance change was calculated by dividing the absolute difference of the dipstick reflectance before and after the reaction by the reflectance before the reaction multiplied by 100%. The reflectance change (∆R [%]) was then plotted against the concentration of the BA.

Calibration and Sensitivity
The effect of different BA concentrations on the reflectance of the dipsticks was studied for four biogenic amines (spermidine, tyramine, putrescine, and histamine). Their calibration plots are presented in Figures 8 and 9. Eleven concentrations (blank, 0.010, 0.020, 0.040, 0.080, 0.10, 0.20, 0.40, 0.60, 0.80, 1.0 mM) were used to cover the whole dynamic range of the dipstick until the saturation of the change of reflectance was reached. Four replicates for each concentration were used. The calibration curves for all BAs are not linear but rather resemble saturation curves. The signal increase is steeper at lower concentrations (0-0.1 mM) than at higher concentrations (0.2-0.6 mM) and reaches saturation for all of the tested BAs at 0.6 mM, except histamine. The dynamic ranges are 0.040-0.60, 0.080-0.60, 0.10-0.60, and 0.10-1.0 mM for SPR, TYR, PUT, and HIS, respectively. The LOD is the concentration which is corresponding to the blank signal plus three standard deviations. The LODs were found to be 30, 30, 80, and 90 µM for SPR, TYR, PUT, and HIS, respectively. The dynamic ranges and LODs of all BAs are summarized in Table 1. All BAs have a similar response and sensitivity, even though one could expect to see a higher sensitivity for the diamines like putrescine and spermidine. Obviously, the average distance to the next proximate S0378 dye (embedded into either the same or neighbor fiber) is larger than the average length of putrescine or spermidine, respectively, so that no additional reaction occurs. Moreover, the steric hindrance of the secondary amino group on a S0378-spermidine conjugate is obviously too high to get access to the rigid conjugated π-system of a neighboring NIR dye. In addition, a further S N 1 reaction of the secondary amino group with another S0378 molecule seems to be prevented. The very similar response of the NIR dye to all BAs is very beneficial for its use in dipsticks. Those are intended as screening tools for the determination of the overall content of BAs in a sample to unveil a suspicious one that then would be further inspected e.g., with HPLC-MS. In this case, a very similar response (i.e., change of reflectance) of the dipsticks in response to all BAs is beneficial to determine the sum content of all BAs, irrespective of their chemical structure. This will reduce potential errors that might occur from overestimation of the S0378 dye towards diamine BAs.
The concentration range of BAs in foods that might be potentially dangerous for health is between 0.3 and 1.0 mM. The LODs and working ranges of the dipsticks are even sufficient to detect concentrations lower than 0.3 mM (Table 1). This is an advantage because uncritical concentrations of BAs as, e.g., naturally occurring in seafood are accessible. Moreover, one can follow the increase of the concentration of BAs during the ageing of seafood using a suitable dilution (see Section 3.7 on quantitation of BAs in real samples). This makes the dipsticks suitable tools to control food freshness or spoilage.

Selectivity
The selectivity of the S0378-CA nanofibers was tested with the following substances: dimethylamine (DMA), triethylamine (TEA), human serum albumin (HSA), and cysteine (CYS) ( Figure S9). These particular substances were chosen to test the selectivity of the response of the dipsticks towards secondary amines, tertiary amines, proteins, and thiols (in absence of BAs). Being nucleophiles, those could interfere in the reaction of S0378 with a BA. The effect of those interferents depending on their concentrations on the reflectance of the dipstick was studied ( Figure 10).
No significant interference on the reflectance is observed for DMA and TEA. The dye does not react with secondary or tertiary amines but only with primary amino groups. This is probably due to the higher steric hindrance of DMA and DEA and the low accessibility of the carbenium ion located between the four methyl groups at the two indole moieties of S0378. The embedding of the dye inside the fibers of the CA hydrogel should also contribute here. CYS only slightly decreases the reflectance response of the dipsticks in general but has no concentration-dependent effect. Although CYS has a primary amino group this group is still lowly protonated at the pH of the reaction (9.7) because its pK a is 10.77 [33]. This means that deprotonated (and hence nucleophilic CYS) is present only to a minute degree. CYS therefore mostly exists as zwitterion in which the amino group is not available to react. Although the thiol group could act as an interfering nucleophile and react with the NIR dye, it obviously is less reactive under the conditions of the dipstick reaction. HSA shows an interference only at relatively high concentrations (0.04-0.2 mM). This could be expected because it has various primary amino groups that could react with the dye. On the other hand, the CA polymer in the dipsticks particularly hinders the access of larger macromolecules like proteins. Therefore, the reaction of free amino groups of HSA is only possible at relatively high concentrations of the protein.
At c HSA > 0.2 mM, the fibers are destroyed. The reason for this is still unknown. The interference of proteins, however, might be relevant for the use of the dipsticks in real samples, but the common sample pretreatments for determination of BAs which use carrez solutions (this work), methanol [20,31,33], or trichloroacetic acid [19] warrant quantitative removal of proteins prior to use of the dipstick. Additionally, the selectivity of the dipsticks towards tyramine in the presence of HSA interferent was tested. HSA (0.04 mM) was added to solutions with increasing concentrations of TYR and ∆R [%] was measured and compared to the ∆R of solutions of TYR of the same concentrations without HSA. The addition of HSA up to 0.04 mM does not affect the calibration plot of tyramine, as it is obvious from Figure 11. Obviously, the reaction rate of S0378 inside the fibers with primary amines is considerably higher than the reaction rate with amines of proteins due to steric hindrance. Hence, the dipsticks react selectively to primary (biogenic) amines as found in food samples, provided that an appropriate sample preparation is applied.

Quantitation of BAs in Real Samples
Finally, the response of the dipsticks to BAs in shrimp samples was tested over a 6-day storage period at room temperature. The extraction of the shrimp samples follows the AOAC method 35.1.32 [31] and standard additions were done. The methanolic extraction procedure works best in that it is fast, simple, and eliminates the interference of proteins to a major degree. Proteins denature and precipitate in methanol and can be removed with filtration. Carrez solution I (potassium ferrocyanide) and Carrez solution II (zinc acetate) were added to the shrimp extract before filtration to completely precipitate the proteins and avoid any interference. Histamine was chosen as BA as the standard to be added because it is the major BA present in seafood samples [28]. For each ageing day, a suitable dilution factor was used to remain in the dynamic range of the dipsticks for determination of HIS. The development of the concentration of the BAs with the ageing of shrimp at room temperature as derived from the standard addition plots are given in Figure S10. The R 2 values for all of the linear fitting plots of the real samples exceed 0.96 (Table 2). These correlation coefficients are good considering that dipsticks are always less reproducible than instrumental analytical methods [19,29]. With respect to shrimp ageing at room temperature, the increase of the HIS concentrations found can also be translated into a total content of biogenic amines (TAC). The TAC is then expressed in equivalents of histamine (µmol HIS/g sample). Since histamine is the major biogenic amine occurring in seafood, its concentration is representative for the TAC [34]. Furthermore, the mean molar mass of the BAs found in food equals the molar mass of histamine. Histamine concentrations found in shrimp during ageing at room temperature were 7.54 ± 0.96, 12.8 ± 0.8 and 21.7 ± 3.2 µmol/g (n = 4, each) on days 0, 1, and 6, respectively, as indicated in Table 2. These results ( Figure S11) agree well with ageing profiles of real shrimp samples found in earlier work [35].

Conclusions
Chromogenic dipsticks based on electrospun CA nanofiber mats doped with S0378 dye are introduced for reflectometric determination of the content of biogenic amines (BAs) in food samples. S0378 is a chameleon amine-reactive dye which changes its color from green to blue when conjugated to primary amino groups. Hence, the reaction of dipsticks with BAs was monitored via reflectance measurement. The dipsticks can also be used for a yes/no qualitative analysis by the naked eye for determination of potentially dangerous concentrations of biogenic amines. Various biogenic amines such as histamine, tyramine, putrescine, and spermidine, respectively, all show an equal reflectance response. The selectivity of the dipsticks towards primary amines over secondary and tertiary amines is very good and only high protein concentrations may interfere. For real samples, this fact is unimportant, since the sample preparation usually involves steps for protein precipitation. Quantitative analysis of BAs in shrimp samples at room temperature successfully delivered a typical profile of the BA concentration upon ageing of the sample, in agreement with previous studies. Hence, these dipsticks show that electrospun nanofibers are very useful sensor materials for monitoring food freshness using innovative and a simple device.

Supplementary Materials:
The following are available online at http://www.mdpi.com/2227-9040/8/4/99/s1, Figure S1: Image of the electrospinning setup with voltage supply and syringe pump, Figure S2: Change of the fluorescence emission of 5 µM S0378 dye upon binding to 5µM Tyramine at 80 • C over time in CHES buffer, Figure S3: Effect of reaction time on the reflectance of the dipstick at 650 nm to HIS solutions of 0, 0.02, 0.04, 0.1, 0.2, 0.4, 0.8 mM, Figure S4: Representative sample for calculation of the pore size as indicated by the Feret diameter, Figure S5: Morphology of S0378-CA nanofibers electrospun for 15 min as taken by SEM, Figure S6: Sketch of cross-sectional view of fused S0378-CA nanofibers, Figure S7: Instrumental setup used for reflectometric measurements, Figure S8: Close-up view on the dipsticks on the sample holder during detection, Figure S9: Structure of the potential interferents tested with the dipsticks, Figure S10: Calibration plots obtained from the change of the reflectance upon ageing of shrimp at room temperature using standard additions and the dilution factors given in Table 2 on days 0, 1, and 6, Figure S11: Bar plot of the TAC of shrimp ageing at room temperature over 6 days. Table S1. Spinning conditions and fiber (mat) characterization data.