Impedimetric Biosensor Based on a Hechtia argentea Lectin for the Detection of Salmonella spp.

: A sensitive electrochemical detection method for Salmonella spp. was described, based on the use of Hechtia argentea lectin immobilised on a screen-printed gold electrode. The lectin was extracted from Hechtia argentea , a plant belonging to the Bromeliaceae family. The lectin with molecular weight near 27.4 kDa showed selectivity towards D-mannose, contained on the lipopolysaccharide cell wall of Salmonella spp. Carbohydrate selectivity of the lectin was measured as a change in impedance with respect to concentration. The binding of the bacteria to the biosensor surface increased impedance with increasing concentrations of Salmonella spp., achieving a linear range of detection of 15–2.57 × 10 7 CFU mL − 1 , with a limit of detection of 5 CFU mL − 1 . Increases in impedance were measured using electrochemical impedance spectroscopy and analysed using Nyquist plots. The biosensor was applied in analysis of hen egg samples, and the results were consistent with those obtained using the o ﬃ cial analysis methodology.


Introduction
Detection of different bacterial species is important in food safety and public health [1], due to their health implications. Salmonella spp. is one of the most frequent pathogens found in foods such as poultry, eggs, dairy products, fruits and vegetables [2,3]. They are facultative anaerobes, and are a Gram-negative bacillus from the family Enterobacteriaceae. S. enterica and S. bongori can cause disease due to their capacity to adapt to a variety of hosts, including humans [4]. Salmonella spp. can cause salmonellosis, affecting millions of people and causing more than 100,000 deaths per year [5].
An alternative to current analysis methods is the use of biosensors, which use selective recognition agents to promote selectivity, reducing erroneous diagnoses with the advantages to provide reliable,

Lectin Extraction and Evaluation
All solutions were prepared in deionised water with resistance not less than 18 MΩ-cm, purified by a Milli-Q system (Millipore, Bedford, MA, USA).
The HA was provided by the Biological Research Center of the Autonomous University of Hidalgo State. HA leaves were dried at room temperature and then pulverized in a mortar. The dried material (10.0 g) was stirred for 60 min in 150 mM NaCl (50.0 mL) (J.T. Baker, Phillipsburg, NJ, USA). The mixture was left for 16 h at 4 • C, and then filtered through gauze. The liquid phase was centrifuged (15 min, 4000 rpm) and stored at −20 • C [34]. Protein content was estimated by the Bradford method using bovine serum albumin (Sigma-Aldrich; St. Louis, MO, USA) as standard [35]. Protein molecular weight was determined by polyacrylamide gel electrophoresis (SDS-PAGE) (11% resolving gel and 4% stacking gel), using Coomassie Brilliant Blue G-250 for protein staining [36].
Lectin presence in HA extracts was confirmed by the evaluation of hemagglutinating activity of the aqueous extract. HA extract (50.0 µL) was mixed with human blood type A, B, and O (50.0 µL each), and the mixture was incubated at 37 • C for 30 min. Agglutination was observed under a microscope at 40× magnification. Negative controls used a 150.0 mM NaCl solution in place of HA extract. Inhibition of hemagglutinating activity was evaluated with the following carbohydrates: N-acetyl-D-glucosamine, N-acetylneuraminic acid, L-fucose, D-fructose, D-galactose, D-glucose, D-maltose, and D-mannose (all Sigma-Aldrich). Inhibition tests were performed following the reported procedure, adding 0.5 M carbohydrate solution (50.0 µL) for both tests. Negative controls used a 150.0 mM NaCl solution in place of carbohydrate [37].

Biosensor Construction
The biosensor was constructed on the surface of a low-temperature curing, screen-printed gold electrode (DropSens 220BT, 4 mm diameter, Asturias, Spain). Gold was used as a working electrode, and silver was used as both the auxiliary and pseudo-reference electrodes.  Once the electrode surface was activated, the HA extract (40.0 µL) was applied and allowed to stand for 1.5 h. The electrode was then immersed in a 20.0 mM ethanolamine (Sigma Aldrich) solution (prepared in deionised water) for 30 min, in order to block the still-active positions. The biosensor was rinsed and stored in PBS at 4 • C prior to use [38].
Micrographs of the biosensor surface were obtained by scanning electron microscopy (SEM; Carl Zeiss, model EVO-50, Oberkochen, Germany). The biosensor was evaluated after analyzing a sample containing 10 5 CFU mL −1 of Salmonella spp.

Impedimetric Measurements
All impedimetric measurements were carried out in a Metrohm Autolab galvanostat potentiostat electrochemical system (model PGSTATAT302N, Amsterdam, The Netherlands), controlled through Nova 2.0 software.
Cyclic voltammetry was used to select the direct current potential used for impedance measurements. Figure 1 shows the cyclic voltamograms of Fe(CN) 6 4− /Fe(CN) 6  Once the electrode surface was activated, the HA extract (40.0 μL) was applied and allowed to stand for 1.5 h. The electrode was then immersed in a 20.0 mM ethanolamine (Sigma Aldrich) solution (prepared in deionised water) for 30 min, in order to block the still-active positions. The biosensor was rinsed and stored in PBS at 4 °C prior to use [38]. Micrographs of the biosensor surface were obtained by scanning electron microscopy (SEM; Carl Zeiss, model EVO-50, Oberkochen, Germany). The biosensor was evaluated after analyzing a sample containing 10 5 CFU mL −1 of Salmonella spp.

Impedimetric Measurements
All impedimetric measurements were carried out in a Metrohm Autolab galvanostat potentiostat electrochemical system (model PGSTATAT302N, Amsterdam, The Netherlands), controlled through Nova 2.0 software.
Cyclic voltammetry was used to select the direct current potential used for impedance measurements. Figure 1 shows the cyclic voltamograms of Fe(CN)6 4− /Fe(CN)6 3− redox couple at different modified electrode surfaces. A reversible Nersitan charge transfer was observed at Au bare surface at 350 mV (vs. Ag) whereas an absence of current peaks for modified surfaces was obtained due to the formation of a coating, indicating an increase in electron transfer resistance. Impedance measurements were performed at the formal potential of the Fe(CN)6 4− /Fe(CN)6 3− redox pair. A direct current potential of 350 mV and an alternating current potential of 5 mV were applied. Impedance was determined by electrochemical impedance spectroscopy (EIS), at 25 frequencies from 0.010 Hz to 100 kHz. A volume of 40.0 μL of either the standard or sample was placed on the biosensor for 15 min at 25 °C, then the electrode was rinsed with PBS solution, and the impedance measurement was performed with 40 μL of the redox probe (5.0 mM potassium hexacyanoferrate (III) +5.0 mM potassium hexacyanoferrate (II) trihydrate) [38]. The impedance spectra were plotted in the form of Nyquist plots.
The biosensor was tested employing carbohydrates, which inhibited agglutination, at concentrations between 1 × 10 −7 -1 × 10 −4 M. Listeria monocytogenes and Salmonella spp. were provided Impedance measurements were performed at the formal potential of the Fe(CN) 6 4− /Fe(CN) 6 3− redox pair. A direct current potential of 350 mV and an alternating current potential of 5 mV were applied. Impedance was determined by electrochemical impedance spectroscopy (EIS), at 25 frequencies from 0.010 Hz to 100 kHz. A volume of 40.0 µL of either the standard or sample was placed on the biosensor for 15 min at 25 • C, then the electrode was rinsed with PBS solution, and the impedance measurement was performed with 40 µL of the redox probe (5.0 mM potassium hexacyanoferrate (III) +5.0 mM potassium hexacyanoferrate (II) trihydrate) [38]. The impedance spectra were plotted in the form of Nyquist plots. The biosensor was tested employing carbohydrates, which inhibited agglutination, at concentrations between 1 × 10 −7 -1 × 10 −4 M. Listeria monocytogenes and Salmonella spp. were provided by the Food Microbiology laboratory at Autonomous University of Hidalgo State. Bacteria were grown in tryptic soy broth (Bioxon Becton Dickinson, Mexico), and incubated at 37 • C for 24 h. Decimal dilutions were made in 0.85% saline solution to obtain a concentration of 10 5 CFU mL −1 of each microorganism, to be analysed by EIS.
To evaluate the proposed biosensor in real samples for comparison with the traditional microbiological culture method, 10 samples of hen eggs were obtained from different commercial establishments in Pachuca, Hidalgo, Mexico. These were kept in polyethylene bags and stored at room temperature from their purchase until the analysis. An egg inoculated with 10 7 CFU mL −1 of Salmonella spp. acted as the positive control, and an egg washed with ethanol acted as the blank/negative control. A single egg was placed in lactose broth (LB; Bioxon) (225 mL) for 60 min at room temperature, then the egg was removed, and the broth was incubated for 24 h at 37 • C for pre-enrichment.

Lectin Evaluation
The presence of lectin in the HA extract was determined by analysing the agglutination of the erythrocytes [41][42][43]. Three types of human blood showed agglutination in the presence of HA extract. Agglutination was observed as accumulations of the erythrocytes, which was not shown in their respective negative controls. The agglutination inhibition test for free carbohydrates is based on the affinity of lectin for these molecules [43][44][45][46]. The results showed that the carbohydrates that inhibited agglutination were N-acetyl-D-glucosamine, N-acetylneuraminic acid and D-mannose, indicating that the lectin presented active sites that allowed it to interact with these carbohydrates.
The total protein content in the HA extract was 131.7 mg L −1 . Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis allowed us to identify the amount of proteins contained in the HA extract, as well as the molecular weight of the protein, when compared to a protein standard of known molecular weight. After staining polyacrylamide gel with Coomassie Brilliant Blue G-250, a single band near 27.4 kDa was observed, indicating that the HA extract could be used without any additional purification process.
The extracted lectin has a similar carbohydrate affinity compared to other lectins. For example, Cratylia mollis lectin (31 kDa) showed an affinity for D-glucose and D-mannose [47]. It was used in the development of an impedimetric biosensor based on self-assembled hybrid cysteine-gold

Lectin Evaluation
The presence of lectin in the HA extract was determined by analysing the agglutination of the erythrocytes [41][42][43]. Three types of human blood showed agglutination in the presence of HA extract. Agglutination was observed as accumulations of the erythrocytes, which was not shown in their respective negative controls. The agglutination inhibition test for free carbohydrates is based on the affinity of lectin for these molecules [43][44][45][46]. The results showed that the carbohydrates that inhibited agglutination were N-acetyl-D-glucosamine, N-acetylneuraminic acid and D-mannose, indicating that the lectin presented active sites that allowed it to interact with these carbohydrates.
The total protein content in the HA extract was 131.7 mg L −1 . Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis allowed us to identify the amount of proteins contained in the HA extract, as well as the molecular weight of the protein, when compared to a protein standard of known molecular weight. After staining polyacrylamide gel with Coomassie Brilliant Blue G-250, a single band near 27.4 kDa was observed, indicating that the HA extract could be used without any additional purification process.
The extracted lectin has a similar carbohydrate affinity compared to other lectins. For example, Cratylia mollis lectin (31 kDa) showed an affinity for D-glucose and D-mannose [47]. It was used in the development of an impedimetric biosensor based on self-assembled hybrid cysteine-gold nanoparticles for the analysis of S. enterica [48]. Also, a Triticum vulgaris lectin (36 kDa), with an affinity for glycoconjugates with N-acetylglucosamine or N-acetylneuraminic acid residues, was used for magnetic separation of S. enterica [49].

Biosensor Impedimetric Evaluation
Impedimetric biosensors are based on the immobilization of a biological recognition element related to the analyte. When recognition occurs on the electrode, changes in the interfacial electron transfer impedance occur. The interfacial changes generated by the recognition processes are detected by EIS [50], and are proportional to the concentration of the analyte [50,51].
The formation of self-assembled monolayers (SAMs) is one of the most-used methods for the immobilization of antibodies in immunoassays. The immobilization of the recognition agent on a surface requires the following characteristics: (1) the recognition element must be oriented with minimum steric impediment to effectively interact with the analyte, and (2) the recognition agent should not lose its ability to interact with the analyte. The main methods of immobilization are the formation of imines with glutaraldehyde and the formation of amides with the addition of EDC/NHS [38,52]. The amide formation offers a high conversion efficiency, mild reaction conditions, and excellent biocompatibility, with little influence on the bioactivity of the target molecules and much cleaner products than other reagents, such as glutaraldehyde [38,53].
In this work, the biosensor was evaluated for measuring the interaction between lectin and carbohydrates that inhibited agglutination in blood. Solutions of N-acetyl-D-glucosamine, N-acetylneuraminic acid, and D-mannose (1 × 10 −7 -1 × 10 −4 M), were analysed, and an increase in impedance with the concentration of carbohydrates was observed ( Figure 3A), while no significant changes were observed during analysis of D-galactose ( Figure 3B). The lectin extracted from HA presented an affinity for N-acetyl-D-glucosamine, N-acetylneuraminic acid and D-mannose. These carbohydrates are contained in the cell wall of some Salmonella spp. [48,54,55] and could be exploited when designing a device for microbiological analysis of Salmonella spp. nanoparticles for the analysis of S. enterica [48]. Also, a Triticum vulgaris lectin (36 kDa), with an affinity for glycoconjugates with N-acetylglucosamine or N-acetylneuraminic acid residues, was used for magnetic separation of S. enterica [49].

Biosensor Impedimetric Evaluation
Impedimetric biosensors are based on the immobilization of a biological recognition element related to the analyte. When recognition occurs on the electrode, changes in the interfacial electron transfer impedance occur. The interfacial changes generated by the recognition processes are detected by EIS [50], and are proportional to the concentration of the analyte [50,51].
The formation of self-assembled monolayers (SAMs) is one of the most-used methods for the immobilization of antibodies in immunoassays. The immobilization of the recognition agent on a surface requires the following characteristics: (1) the recognition element must be oriented with minimum steric impediment to effectively interact with the analyte, and (2) the recognition agent should not lose its ability to interact with the analyte. The main methods of immobilization are the formation of imines with glutaraldehyde and the formation of amides with the addition of EDC/NHS [38,52]. The amide formation offers a high conversion efficiency, mild reaction conditions, and excellent biocompatibility, with little influence on the bioactivity of the target molecules and much cleaner products than other reagents, such as glutaraldehyde [38,53].
In this work, the biosensor was evaluated for measuring the interaction between lectin and carbohydrates that inhibited agglutination in blood. Solutions of N-acetyl-D-glucosamine, N-acetylneuraminic acid, and D-mannose (1 × 10 −7 -1 × 10 −4 M), were analysed, and an increase in impedance with the concentration of carbohydrates was observed ( Figure 3A), while no significant changes were observed during analysis of D-galactose ( Figure 3B). The lectin extracted from HA presented an affinity for N-acetyl-D-glucosamine, N-acetylneuraminic acid and D-mannose. These carbohydrates are contained in the cell wall of some Salmonella spp. [48,54,55] and could be exploited when designing a device for microbiological analysis of Salmonella spp. The biosensor was applied for the analysis of independent solutions of a Gram-positive (L. monocytogenes) and a Gram-negative (Salmonella spp.) bacteria (each 10 5 CFU mL −1 ). The analysis of L. monocytogenes did not reveal any change in impedance ( Figure 4A). Although a positive response was expected in Listeria spp., it being a Gram-positive bacterium with a cell wall containing high levels of peptidoglycan, a linear polymer made up of N-acetyl-D-glucosamine and N-acetylmuramic acid that interacts with amino acids [56]. However, since peptidoglycan is not the terminal carbohydrate, there is no interaction with the recognition agent from the HA lectin. An increase in The biosensor was applied for the analysis of independent solutions of a Gram-positive (L. monocytogenes) and a Gram-negative (Salmonella spp.) bacteria (each 10 5 CFU mL −1 ). The analysis of L. monocytogenes did not reveal any change in impedance ( Figure 4A). Although a positive response was expected in Listeria spp., it being a Gram-positive bacterium with a cell wall containing high levels of peptidoglycan, a linear polymer made up of N-acetyl-D-glucosamine and N-acetylmuramic acid that interacts with amino acids [56]. However, since peptidoglycan is not the terminal carbohydrate, there is no interaction with the recognition agent from the HA lectin. An increase in impedance was observed when Salmonella spp. was analysed ( Figure 4B). This impedance increase can be attributed to the lectin-Salmonella spp. interaction; the bacterium contains an external membrane made up of lipopolysaccharides containing different terminal carbohydrates, including D-mannose as the terminal carbohydrate [30].  The interactions that occur in the recognition between a lectin and carbohydrates are hydrogen bonding, metal coordination, van der Waals forces and hydrophobic interactions [26]. In microbial biosensors, the interaction occurs between microorganisms (lipopolysaccharides in the cell wall) and the recognition element (lectin) [57]. Figure 5 shows the recognition scheme for Salmonella spp. the presence of a coating (SAMs) on the surface of the electrode changes both the interfacial capacitance and the speed of electron transfer, causing an increase in impedance. This increase is because the bacteria, bound to the surface through interaction with lectin, act as a barrier for the transfer of electrons between the redox probe (Fe(CN)6 4− /Fe(CN)6 3− ) and the biosensor. Based on the results of agglutination inhibition and the change in the impedance of the device, it is probable that the lectin contained in HA extract interacts with D-mannose contained in the The interactions that occur in the recognition between a lectin and carbohydrates are hydrogen bonding, metal coordination, van der Waals forces and hydrophobic interactions [26]. In microbial biosensors, the interaction occurs between microorganisms (lipopolysaccharides in the cell wall) and the recognition element (lectin) [57]. Figure 5 shows the recognition scheme for Salmonella spp. the presence of a coating (SAMs) on the surface of the electrode changes both the interfacial capacitance and the speed of electron transfer, causing an increase in impedance. This increase is because the bacteria, bound to the surface through interaction with lectin, act as a barrier for the transfer of electrons between the redox probe (Fe(CN) 6 4− /Fe(CN) 6 3− ) and the biosensor.  The interactions that occur in the recognition between a lectin and carbohydrates are hydrogen bonding, metal coordination, van der Waals forces and hydrophobic interactions [26]. In microbial biosensors, the interaction occurs between microorganisms (lipopolysaccharides in the cell wall) and the recognition element (lectin) [57]. Figure 5 shows the recognition scheme for Salmonella spp. the presence of a coating (SAMs) on the surface of the electrode changes both the interfacial capacitance and the speed of electron transfer, causing an increase in impedance. This increase is because the bacteria, bound to the surface through interaction with lectin, act as a barrier for the transfer of electrons between the redox probe (Fe(CN)6 4− /Fe(CN)6 3− ) and the biosensor. Based on the results of agglutination inhibition and the change in the impedance of the device, it is probable that the lectin contained in HA extract interacts with D-mannose contained in the lipopolysaccharide of Salmonella spp. Based on the results of agglutination inhibition and the change in the impedance of the device, it is probable that the lectin contained in HA extract interacts with D-mannose contained in the lipopolysaccharide of Salmonella spp. Figure 6A shows an SEM micrograph of gold electrodes cured at low temperature, where an irregular surface with small agglomerations acting as single microelectrodes can be observed. The micrograph of the biosensor employed for analysis of 10 5 CFU mL −1 Salmonella spp. ( Figure 6B) shows a layer formation on the electrode surface, and the presence of Salmonella spp. bacteria. This can be attributed to the lectin-carbohydrate interaction.
Chemosensors 2020, 8, x FOR PEER REVIEW 7 of 12 Figure 6A shows an SEM micrograph of gold electrodes cured at low temperature, where an irregular surface with small agglomerations acting as single microelectrodes can be observed. The micrograph of the biosensor employed for analysis of 10 5 CFU mL −1 Salmonella spp. ( Figure 6B) shows a layer formation on the electrode surface, and the presence of Salmonella spp. bacteria. This can be attributed to the lectin-carbohydrate interaction.

Sample Analysis
The biosensor was applied for analysis of hen eggs. The proposed methodology for the EIS analysis involves pre-enrichment, which restores the damaged bacterial cells to a stable physiological condition, then a selective enrichment used to increase the population of Salmonella spp. by inhibiting the growth of other organisms present in the sample, subsequently reducing interferences from other bacteria [6,8,21]. The analysis for Salmonella spp. was constructed using the Nyquist plots, showing that, in a concentration range of 10 3 -10 7 CFU mL −1 , there is an increase in impedance with respect to the logarithm of the concentration of Salmonella spp. (Figure 6). The biosensor resistance (ΔR) vs. logarithm of the concentration of Salmonella spp. were linear in the same interval (Figure 7), with a limit of detection of 5 CFU mL −1 and a limit of quantification of 15 CFU mL −1 (R 2 = 0.9902). The reproducibility was evaluated analysing with three independent biosensors samples containing 0, 10 5 and 10 6 CFU mL −1 , the relative standard deviation obtained from the analysis was 3.45, 1.69 and 0.90%, respectively. The values obtained were adequate for a biosensor.

Sample Analysis
The biosensor was applied for analysis of hen eggs. The proposed methodology for the EIS analysis involves pre-enrichment, which restores the damaged bacterial cells to a stable physiological condition, then a selective enrichment used to increase the population of Salmonella spp. by inhibiting the growth of other organisms present in the sample, subsequently reducing interferences from other bacteria [6,8,21]. The analysis for Salmonella spp. was constructed using the Nyquist plots, showing that, in a concentration range of 10 3 -10 7 CFU mL −1 , there is an increase in impedance with respect to the logarithm of the concentration of Salmonella spp. (Figure 6). The biosensor resistance (∆R) vs. logarithm of the concentration of Salmonella spp. were linear in the same interval (Figure 7), with a limit of detection of 5 CFU mL −1 and a limit of quantification of 15 CFU mL −1 (R 2 = 0.9902). The reproducibility was evaluated analysing with three independent biosensors samples containing 0, 10 5 and 10 6 CFU mL −1 , the relative standard deviation obtained from the analysis was 3.45, 1.69 and 0.90%, respectively. The values obtained were adequate for a biosensor.
Lectins are selective recognition elements for the detection of pathogens, in addition, the collection and extraction processes were not complicated. Lectins were investigated for the detection of some pathogens due to their ability to bonding selectively to the surfaces of viruses and bacteria [58]. Table 1 shows some lectins applied to analyse pathogens, Concanavalin is the most studied lectin and it was used for the detection of Escherichia coli. Currently, the development of electrochemical biosensors based on different lectins for the detection of different pathogens important to human health is an interesting area in biosensing [59]. that, in a concentration range of 10 3 -10 7 CFU mL −1 , there is an increase in impedance with respect to the logarithm of the concentration of Salmonella spp. (Figure 6). The biosensor resistance (ΔR) vs. logarithm of the concentration of Salmonella spp. were linear in the same interval (Figure 7), with a limit of detection of 5 CFU mL −1 and a limit of quantification of 15 CFU mL −1 (R 2 = 0.9902). The reproducibility was evaluated analysing with three independent biosensors samples containing 0, 10 5 and 10 6 CFU mL −1 , the relative standard deviation obtained from the analysis was 3.45, 1.69 and 0.90%, respectively. The values obtained were adequate for a biosensor.  Ricinus communis N-acetyl-D-galactosamine and D-galactose E. coli DH5α Enterobacter cloacae, and Bacillus subtilis

EIS 5 CFU/mL This work
On the other hand, the biosensor based on lectin obtained from Hechtia argentea is competitive compared to other lectins used for bacterial analysis (Table 1). Furthermore, the LOD obtained is comparable with other biosensors based on specific antigens. Yang et al. [65] developed a biosensor based on a self-assembled gold nanoparticle monolayer and grafted ethylenediamine on a vitreous carbon electrode, immobilizing specific monoclonal antibodies to Salmonella spp. in gold nanoparticles, achieving a limit of detection of 100 CFU mL −1 .
The proposed biosensor was used to analyse 10 hen eggs, including negative and positive control samples. For all test samples and the negative control sample, a change in impedance in the analysis was not observed using the biosensor. The positive control showed the expected change in impedance. The microbiological analysis was congruent, and presumptive colonies of Salmonella spp. were not observed. The results obtained are preliminary, but there must be test more samples, including other analytical matrices, in order to evaluate if the biosensor is robust.

Conclusions
Hechtia argentea contains a lectin with a molecular weight near 27.4 kDa that is selective towards N-acetyl-D-glucosamine, N-acetylneuraminic acid, and D-mannose, allowing recognition of Salmonella spp. The biosensor achieved a limit of detection of 5 CFU mL −1 and a linear range of detection of 15-2.57 × 10 7 CFU mL −1 . The biosensor was applied successfully to the analysis of hen egg samples, and the results obtained were consistent with those obtained from the official analysis methodology. The proposed technique presents advantages over the official methodology for the detection of Salmonella spp., such as higher simplicity and shorter analysis time.