Evaluation of a Reduced Graphene Oxide-Sb Nanoparticles Electrochemical Sensor for the Detection of Cadmium and Lead in Chamomile Tea

: The development of electroanalytical sensors for heavy metals detection in complex matrices holds great interest. Herein, a sensor based on a reduced graphene oxide (rGO) modiﬁed with antimony nanoparticles (Sb) was developed for the electrochemical detection of divalent cadmium ions (Cd 2 + ) and lead ions (Pb 2 + ). The simultaneous determination of both metals covered a range of 0.1 to 3.0 µ mol L − 1 , with limits of detection (LOD) of 70.03 and 45.50 nmol L − 1 for Cd 2 + and Pb 2 + , respectively. For the individual detection, LOD of 20.50 nmol L − 1 (Cd 2 + ) and 2.01 nmol L − 1 (Pb 2 + ) were found. The analytical performance of this new sensor in detecting both metals in chamomile tea samples was satisfactorily evaluated.


Introduction
Heavy metals are well known for their relevance and extensive use in technological advances (or advancements). However, their wide distribution in the industrial, medical, agricultural, and food sectors raises the concern over the possibility of negative effects on the environment and human health [1]. Mainly in the agricultural sector, the irrigation of wastewater, vehicular exhaustion, prolong application of chemical fertilizers, and local industrial activities play a major role as the contamination route of the soil, revealing the direct or indirect influence of anthropogenic activities [2][3][4]. Several studies have already quoted the presence and influence of these contaminants, appointing to bioaccumulation as the major threat. This process is directly associated with the number of trophic levels between the origin of the food contamination and its final consumer, presenting a major problem for the agricultural production, thus affecting both quality and safety in consumption [5][6][7]. On the other side, the chronic intake of contaminated food, even in low doses, may induce neurologic, renal, osteoarticular disorders, besides leading to genetic and epigenetic dysfunctions, and associated as a risk factor for the development of some forms of cancer [8][9][10].
Being the second most consumed beverage worldwide, with its production reaching 5.8 million metric tons in 2018, tea is a possible route of contamination [11,12]. These data show its economic and cultural impact, enhancing the assurance of quality and safety in general tea production. Chamomile is one of the most popular herbal teas, most commonly represented by two varieties, Roman chamomile (Chamaemelum nobile) and German chamomile (Matricaria chamomilla) [13,14]. Its medical use dates back to ancient history, being one of the oldest medicinal herbs in our culture. The well documented medical properties as anti-inflammatory [15], antidiarrheal and antioxidant activities [16], potential management in type 2 diabetes [17], as well as its calming effects contributing for treatment of sleep disorders and anxiety [13,18], are mainly associated to the variety of phenolic compounds (p. Ex. Cyclic voltammetry (CV) and square wave anodic stripping voltammetry (SWASV) experiments were performed using an electrochemical system model Autolab 128N (Eco Chemie, Utrecht, The Netherlands) equipped with NOVA 2.1 software (Eco Chemie, Utrecht, The Netherlands). All electrochemical tests were conducted in a conventional three-electrode system with a bare glassy carbon electrode (GC), GC/GO, GC/rGO, or GC/rGO-SbNPs as the working electrode, Ag/AgCl/KCl (3.0 mol L −1 ) as the reference electrode, and a platinum plate as the auxiliary electrode.

Synthesis of the Nanocomposites
Initially, for the chemical reduction step, a suspension of GO and sodium dodecyl sulfate (SDS) was prepared in a 10:4 ratio in an ethanol medium, followed by a sonication step for 20 min. Excess of sodium borohydride (NaBH 4 ) was then added (16 mg) and a 20 min sonication was carried out. The solution was then centrifuged and cleaned several times with ethanol. The resulting rGO was dried at 60 • C and dispersed in ultrapure water at a concentration of 1.0 mg mL −1 . For optimization, the synthesis of the nanocomposite rGO-SbNPs was performed with four different antimony chloride (SbCl 3 )/GO ratios as follows: 20%, 30%, 40%, and 50% of SbCl 3 .
The solution containing SbCl 3 was added dropwise (≈1 drop per second) to the rGO suspension under constant stirring. The product was then centrifuged for 10 min (3000 rpm) and washed four times with ethanol pure grade (the supernatant became colorless). The dried material was dispersed in ultrapure water (0.3 mg mL −1 ) and kept under refrigeration for further use. The rGO composite was synthesized as described above without the addition of the metallic salt. The materials were sonicated daily in order to prevent precipitation and to keep homogeneity of the film.

Procedures
The surface morphology was characterized by scanning electron microscopy (SEM) coupled to an electron gun (FEG-SEM), JEOL MODELO JSM7500F, at the Advanced Microscopy Laboratory (LMA) of the Institute of Chemistry-UNESP Araraquara. SEM micrographs were also obtained by Quanta 200 (FEI Company, Hillsboro, OR, USA) at the Electron Microscope Center of the Institute of Biosciences of Botucatu, UNESP.
For the electrochemical characterization of the composite, a CV experiment was conducted by comparing the GC/rGO and GC/rGO/SbNPs electrode's voltammetric profiles in HCl 0.5 mol L −1 solution, with a scan rate of 50 mV s −1 within the potential range of −0.6 to +0.2 V. SWASV measurements were recorded in a 0.1 mol L −1 PBS solution (pH 3.0), within a potential range of −1.0 to −0.1 V, with an accumulation potential of −1.0 V for 120 s, pulse amplitude of 90 mV and 80 Hz frequency, containing 10.0 µmol L −1 of Cd 2+ and Pb 2+ . These measurements were carried out at room temperature.

Electrodes Preparation
The glassy carbon electrodes (GC) were polished using 0.5 µm alumina slurry. Then, the electrodes were sonicated for 5 min in ethanol and 5 min in ultrapure water and dried at room temperature. Finally, 10 µL of the composites were dropped on the surfaces of the cleaned electrodes and dried at room temperature.

Real Tea Samples
A commercial tea bag of chamomile (acquired at a local market) containing 1 g of the sample was boiled within 50 mL ultrapure water and left to soak for 20 min. The solution was transferred to a volumetric flask and nitric acid was added to achieve a 0.6% (v/v) solution [34]. The solution was kept under refrigeration prior to analysis.

Morphologic Characterization of Nanocomposites
The surface morphology of rGO and rGO-SbNPs nanocomposites was characterized by scanning electron microscopy (FEG-SEM), as shown in Figure 1. The characteristic morphological structure of rGO is observed in Figure 1a. Its precursor, GO tends to form a mixture of single and multiple layers (sheets) as 2D material, the stacking of single sheets via electrons π-π is commonly expected for this material. After the chemical reduction step, the rGO structure presents a distorted and wrinkled sheet of graphene due to unstacking. This process aims to decrease functional groups containing oxygen (mainly carbonyl and hydroxy), thus, making the material exhibit a typical twisted and wrinkled structure, which boosts its electronic properties for a more similar behavior of unmodified graphene. Figure 1b shows the integration of Sb nanoparticles on the rGO nanosheet surface. The morphologic analysis of this nanocomposite revealed nanoparticles with their size ranging from 50 to 150 nm. (sheets) as 2D material, the stacking of single sheets via electrons π-π is commonly expected for this material. After the chemical reduction step, the rGO structure presents a distorted and wrinkled sheet of graphene due to unstacking. This process aims to decrease functional groups containing oxygen (mainly carbonyl and hydroxy), thus, making the material exhibit a typical twisted and wrinkled structure, which boosts its electronic properties for a more similar behavior of unmodified graphene. Figure 1b shows the integration of Sb nanoparticles on the rGO nanosheet surface. The morphologic analysis of this nanocomposite revealed nanoparticles with their size ranging from 50 to 150 nm.

Electrochemical Characterization
The electrochemical characterization of the GC/rGO-SbNPs electrode was evaluated using a CV experiment in a 0.5 mol L −1 HCl solution. The voltammograms, shown in Figure 2, indicate the presence of Sb nanoparticles on the rGO surface (solid line). Two oxidation processes regarding SbNPs were observed at −16.5 and 100.7 mV. In the reverse scan, the GC/rGO-SbNPs electrode showed a reduction peak at −331.4 mV. Both oxidation and reduction processes for Sb were already extensively observed in the literature [35,36]. In contrast, the GC/rGO electrode (dashed line) did not present any visible oxidation processes in the voltammetric profile.

Electrochemical Characterization
The electrochemical characterization of the GC/rGO-SbNPs electrode was evaluated using a CV experiment in a 0.5 mol L −1 HCl solution. The voltammograms, shown in Figure 2, indicate the presence of Sb nanoparticles on the rGO surface (solid line). Two oxidation processes regarding SbNPs were observed at −16.5 and 100.7 mV. In the reverse scan, the GC/rGO-SbNPs electrode showed a reduction peak at −331.4 mV. Both oxidation and reduction processes for Sb were already extensively observed in the literature [35,36]. In contrast, the GC/rGO electrode (dashed line) did not present any visible oxidation processes in the voltammetric profile. (sheets) as 2D material, the stacking of single sheets via electrons π-π is commonly expected for this material. After the chemical reduction step, the rGO structure presents a distorted and wrinkled sheet of graphene due to unstacking. This process aims to decrease functional groups containing oxygen (mainly carbonyl and hydroxy), thus, making the material exhibit a typical twisted and wrinkled structure, which boosts its electronic properties for a more similar behavior of unmodified graphene. Figure 1b shows the integration of Sb nanoparticles on the rGO nanosheet surface. The morphologic analysis of this nanocomposite revealed nanoparticles with their size ranging from 50 to 150 nm.

Electrochemical Characterization
The electrochemical characterization of the GC/rGO-SbNPs electrode was evaluated using a CV experiment in a 0.5 mol L −1 HCl solution. The voltammograms, shown in Figure 2, indicate the presence of Sb nanoparticles on the rGO surface (solid line). Two oxidation processes regarding SbNPs were observed at −16.5 and 100.7 mV. In the reverse scan, the GC/rGO-SbNPs electrode showed a reduction peak at −331.4 mV. Both oxidation and reduction processes for Sb were already extensively observed in the literature [35,36]. In contrast, the GC/rGO electrode (dashed line) did not present any visible oxidation processes in the voltammetric profile.

Performance of GC/rGO-SbNPs Sensor for the Analysis of Cd 2+ and Pb 2+
The stripping analysis for 10.0 µmol L −1 of Cd 2+ and Pb 2+ was conducted in a 0.1 mol L −1 PBS solution at pH 3.0, for the electrochemical comparison of the electrodes GC, GC/GO, GC/rGO, and GC/rGO-SbNPs.
As shown in Figure 3, the oxidation peaks for cadmium and lead ions are located near peak P1 at E pa1 = −0.728 V (Cd 2+ ) and peak P2 at E pa2 = −0.481 V (Pb 2+ ) vs. Ag/AgCl/KCl (3.0 mol L −1 ) for the four studied electrodes. The proposed electrode, GC/rGO-SbNPs (curve d), presented the highest anodic peak currents for both metals when compared to the other studied electrodes, with an increase of approximately 2.4 times for the cadmium and 1.3 times for the lead ions response when compared to GC/rGO electrode anodic peak current. This increase in the response signal for both metals is associated with the synergistic effect of rGO with SbNPs, appointing itself as a new material for the determination of Cd 2+ and Pb 2+ ions on tea samples.

Performance of GC/rGO-SbNPs Sensor for the Analysis of Cd 2+ and Pb 2+
The stripping analysis for 10.0 μmol L −1 of Cd 2+ and Pb 2+ was conducted in a 0.1 mol L −1 PBS solution at pH 3.0, for the electrochemical comparison of the electrodes GC, GC/GO, GC/rGO, and GC/rGO-SbNPs.
As shown in Figure 3, the oxidation peaks for cadmium and lead ions are located near peak P1 at Epa1 = −0.728 V (Cd 2+ ) and peak P2 at Epa2 = −0.481 V (Pb 2+ ) vs. Ag/AgCl/KCl (3.0 mol L −1 ) for the four studied electrodes. The proposed electrode, GC/rGO-SbNPs (curve d), presented the highest anodic peak currents for both metals when compared to the other studied electrodes, with an increase of approximately 2.4 times for the cadmium and 1.3 times for the lead ions response when compared to GC/rGO electrode anodic peak current. This increase in the response signal for both metals is associated with the synergistic effect of rGO with SbNPs, appointing itself as a new material for the determination of Cd 2+ and Pb 2+ ions on tea samples.

Electrode Film Optimization
In order to maximize the SWASV response, the parameters involving the nanocomposite synthesis were optimized. Thus, the influence of the SbCl3/GO ratio during synthesis was analyzed by varying the proportion for 20, 30, 40, and 50% (m/m) compared to a fixed 20 mg of GO. Thus, Figure 4 presents the highest anodic peak current (Ipa), for both metals, at a 30% nanocomposite ratio.
The film concentration of the modified electrode was also evaluated by SWASV experiments (figure not shown). The concentrations evaluated were 12.5, 25.0, 50.0, and 75.0 µg mL −1 . The increase of the stripping peak current for both metals (Cd 2+ and Pb 2+ ) was observed at 12.5 µg mL −1 of the film concentration, followed by a decrease in the electrode response when the composite concentration was raised. High concentrations of the nanocomposite may block the electron-transfer process through the electrode/electrolyte interface [37]. Therefore, the rGO-SbNPs (30%) at 12.5 µg mL −1 was used in further experiments.

Electrode Film Optimization
In order to maximize the SWASV response, the parameters involving the nanocomposite synthesis were optimized. Thus, the influence of the SbCl 3 /GO ratio during synthesis was analyzed by varying the proportion for 20, 30, 40, and 50% (m/m) compared to a fixed 20 mg of GO. Thus, Figure 4 presents the highest anodic peak current (I pa ), for both metals, at a 30% nanocomposite ratio.
The film concentration of the modified electrode was also evaluated by SWASV experiments (figure not shown). The concentrations evaluated were 12.5, 25.0, 50.0, and 75.0 µg mL −1 . The increase of the stripping peak current for both metals (Cd 2+ and Pb 2+ ) was observed at 12.5 µg mL −1 of the film concentration, followed by a decrease in the electrode response when the composite concentration was raised. High concentrations of the nanocomposite may block the electron-transfer process through the electrode/electrolyte interface [37]. Therefore, the rGO-SbNPs (30%) at 12.5 µg mL −1 was used in further experiments.

SWASV and Supporting Electrolyte Optimization
A satisfactory performance of stripping analysis is directly linked with the parameters of the SWASV technique. As having a sensitive and linear response when detecting trace levels of heavy metals (as any possible contaminants), the optimization of SWASV parameters is required. The optimized SWASV parameters were frequency, pulse amplitude, accumulation time, and deposition potential at a fixed 10.0 µmol L −1 of Cd 2+ and Pb 2+ concentration in 0.1 mol L −1 PBS pH 3.0. Table 1 summarizes the range of the evaluated and optimized values found for each parameter. Regarding the square wave voltammetry (SWV) detection, the frequency was optimized in the range of 10 to 90 Hz, the pulse amplitude from 15 to 100 mV. The deposition potential was the first optimized anodic stripping parameter, ranging from −1.3 to −0.8 V, reaching the maximum response for both metals at −1.2 V. The second optimized anodic stripping parameter was the accumulation time, ranging from 45 to 150 s, reaching the maximum response at 150 s. The accumulation time increases the signal for heavy metals detection, due to a rise in the accumulation of metal ions on the electrode surface over time. However, it can be a time-consuming approach. As Wang et al. (2018) reported [38], after a 120-s accumulation time, the increase of the response was not so significant, due to the saturation of the enriched ions on the electrode surface. In this way, for the subsequent experiments, the accumulation time of 120 s at −1.2 V was employed.
The pH variation during stripping analysis plays an important role in the detection of heavy metals [23], as high pH values may cause the precipitation of metals in its oxide form [39]. Therefore, the pH of the PBS buffer was investigated for both metals in the range of 2.0 to 6.0, with its maximum response at pH 3.0.

SWASV and Supporting Electrolyte Optimization
A satisfactory performance of stripping analysis is directly linked with the parameters of the SWASV technique. As having a sensitive and linear response when detecting trace levels of heavy metals (as any possible contaminants), the optimization of SWASV parameters is required. The optimized SWASV parameters were frequency, pulse amplitude, accumulation time, and deposition potential at a fixed 10.0 µmol L −1 of Cd 2+ and Pb 2+ concentration in 0.1 mol L −1 PBS pH 3.0. Table 1 summarizes the range of the evaluated and optimized values found for each parameter. Regarding the square wave voltammetry (SWV) detection, the frequency was optimized in the range of 10 to 90 Hz, the pulse amplitude from 15 to 100 mV. The deposition potential was the first optimized anodic stripping parameter, ranging from −1.3 to −0.8 V, reaching the maximum response for both metals at −1.2 V. The second optimized anodic stripping parameter was the accumulation time, ranging from 45 to 150 s, reaching the maximum response at 150 s. The accumulation time increases the signal for heavy metals detection, due to a rise in the accumulation of metal ions on the electrode surface over time. However, it can be a time-consuming approach. As Wang et al. (2018) reported [38], after a 120-s accumulation time, the increase of the response was not so significant, due to the saturation of the enriched ions on the electrode surface. In this way, for the subsequent experiments, the accumulation time of 120 s at −1.2 V was employed.
The pH variation during stripping analysis plays an important role in the detection of heavy metals [23], as high pH values may cause the precipitation of metals in its oxide form [39]. Therefore, the pH of the PBS buffer was investigated for both metals in the range of 2.0 to 6.0, with its maximum response at pH 3.0.

Calibration Curves
The calibration curves for Cd 2+ and Pb 2+ were built in two different experiments under optimized conditions as described earlier. In the first one, both metals were detected simultaneously in the range of 0.1 to 3.0 µmol L −1 , as shown in Figure 5a. Despite the higher response for Pb 2+ , both metals presented a linear response of the anodic peak current vs. metal concentration, as shown in Figure 5b.

Calibration Curves
The calibration curves for Cd 2+ and Pb 2+ were built in two different experiments under optimized conditions as described earlier. In the first one, both metals were detected simultaneously in the range of 0.1 to 3.0 µmol L −1 , as shown in Figure 5a. Despite the higher response for Pb 2+ , both metals presented a linear response of the anodic peak current vs. metal concentration, as shown in Figure 5b. The second experiment consisted of building both calibration curves individually, as shown in Figure 6. The response Cd 2+ ions were obtained in the range of 50 to 120 nmol L −1 (Figure 6a) and Pb 2+ within the range of 5 to 60 nmol L −1 (Figure 6b).  Table 2 summarizes the method of detection (simultaneous/individual), equation of calibration curve, linear range, linearity (R 2 ), and obtained LOD in nmol L −1 of the experiments described above. The second experiment consisted of building both calibration curves individually, as shown in Figure 6. The response Cd 2+ ions were obtained in the range of 50 to 120 nmol L −1 (Figure 6a) and Pb 2+ within the range of 5 to 60 nmol L −1 (Figure 6b).

Calibration Curves
The calibration curves for Cd 2+ and Pb 2+ were built in two different experiments under optimized conditions as described earlier. In the first one, both metals were detected simultaneously in the range of 0.1 to 3.0 µmol L −1 , as shown in Figure 5a. Despite the higher response for Pb 2+ , both metals presented a linear response of the anodic peak current vs. metal concentration, as shown in Figure 5b. The second experiment consisted of building both calibration curves individually, as shown in Figure 6. The response Cd 2+ ions were obtained in the range of 50 to 120 nmol L −1 (Figure 6a) and Pb 2+ within the range of 5 to 60 nmol L −1 (Figure 6b).  Table 2 summarizes the method of detection (simultaneous/individual), equation of calibration curve, linear range, linearity (R 2 ), and obtained LOD in nmol L −1 of the experiments described above.  Table 2 summarizes the method of detection (simultaneous/individual), equation of calibration curve, linear range, linearity (R 2 ), and obtained LOD in nmol L −1 of the experiments described above. The development of electrochemical sensors based on rGO and metallic nanoparticles for selective detection of Cd 2+ and Pb 2+ have been widely investigated. Wu et al. [40] developed an aluminum-silicon carbide-rGO nanocomposite electrode with LODs down to 19.13 nmol L −1 for Cd 2+ and 6.27 nmol L −1 for Pb 2+ .
Lee et al. [41] developed a similar model compared to the presented study. A rGO modified with Sn nanoparticles-based electrode achieved LODs of 0.63 nmol L −1 for Cd 2+ and 0.60 nmol L −1 for Pb 2+ .
Sang et al. [42] tested the sensor based on rGO modified with AgNPs. Through SWASV experiments, the developed sensor exhibited excellent electrochemical activity and sensitivity towards the detection of several heavy metal ions, as Cd 2+ and Pb 2+ LODs of 254.0 and 141.0 nmol L −1 , respectively.
Liu et al. [43] modified the GC electrode with liquid phase exfoliated graphene nanosheets, prepared through a one-step exfoliation. The developed sensors showed increased response for the electrochemical signals for Cd 2+ and Pb 2+ , with noteworthy signal amplification effects. The LODs determined by this sensor were 11.60 nmol L −1 for Cd 2+ and 8.93 nmol L −1 for Pb 2+ .
The GC/rGO-SbNPs sensor developed in this work has similar LODs for Cd 2+ and Pb 2+ as reported in the literature. Although there are many important types of research in the development of new electroanalytical sensors towards heavy metal detection, the practical application of such sensors is required. In that way, this study evaluated the selective determination of the metals in question in infused chamomile tea samples.
The reproducibility of the GC/rGO-SbNPs electrode was also investigated. For this study, five experiments were measured, in which each experiment consisted of ten sequential SWASV voltammograms. These experiments were performed on different days, in which the electrodes were prepared day-to-day, in other words, using different films. The SWASV voltammograms were performed under the optimized conditions containing 0.5 µmol L −1 of Cd 2+ and Pb 2+ . The calculated standard deviations were 2.8% and 1.9% for Cd 2+ and Pb 2+ , respectively.

Interference Study
In aromatic beverages, such as chamomile tea, some common interferents may be present. Thus, the recovery signal of the voltammetric response for Cd 2+ and Pb 2+ was tested in the absence and the presence of 1.0 µmol L −1 of some common interferents.
Caffeine, an alkaloid, is one of the most common components in tea [44]; as the proposed analytical method does not incinerate/destroy the organic molecules in the matrix, its effect on the metal signal was evaluated. The interfering caffeine did not shift drastically and influence the response for both metals, holding a deviation of ±10% from the original signal.
The matrix evaluated was prepared using drinking water, which may contain Hg 2+ and Cu 2+ ions [45]. When tested, however, both ions had similar behavior as caffeine and did not influence significantly the signal of Cd 2+ and Pb 2+ .
Although Ag 2+ ions are not a common inorganic contaminant in tea samples, it has an incisive influence on the oxidation processes for both metals, exceeding ±20% from the original signal. The results are presented in Figure 7.  Table 3 summarizes the results for the detection of Cd 2+ and Pb 2+ in the chamomile tea sample (infusion). For the detection of both metals, 5 mL of the previously prepared tea was diluted in 15 mL of PBS (pH 3.0), this solution was enriched with 0.5 µmol L −1 of both metals. The determination was conducted using the standard addition method by spiking the sample with metal standards.

Real Samples Analysis
According to the results, there was not a significant difference between the added and found concentrations of Cd 2+ and Pb 2+ , the relative errors within ±10% range. Based on these results, the proposed GC/rGO-SbNPs could be used for the selective detection of the metals in tea samples.

Conclusions
In this study, an rGO-SbNPs nanocomposite was designed and tested in the selective detection of Cd 2+ and Pb 2+ . Both individual and simultaneous determination of heavy metal ions were investigated. The proposed sensor showed good limits of detection compared to similar methodologies reported in the literature. Furthermore, the GC/rGO-SbNPs electrode presented a practical application on the detection of heavy metal ions in a complex matrix, such as chamomile tea. The SWASV experiments did not reveal significant influence on the signals of Cd 2+ and Pb 2+ , for  Table 3 summarizes the results for the detection of Cd 2+ and Pb 2+ in the chamomile tea sample (infusion). For the detection of both metals, 5 mL of the previously prepared tea was diluted in 15 mL of PBS (pH 3.0), this solution was enriched with 0.5 µmol L −1 of both metals. The determination was conducted using the standard addition method by spiking the sample with metal standards. According to the results, there was not a significant difference between the added and found concentrations of Cd 2+ and Pb 2+ , the relative errors within ±10% range. Based on these results, the proposed GC/rGO-SbNPs could be used for the selective detection of the metals in tea samples.

Conclusions
In this study, an rGO-SbNPs nanocomposite was designed and tested in the selective detection of Cd 2+ and Pb 2+ . Both individual and simultaneous determination of heavy metal ions were investigated. The proposed sensor showed good limits of detection compared to similar methodologies reported in the literature. Furthermore, the GC/rGO-SbNPs electrode presented a practical application on the detection of heavy metal ions in a complex matrix, such as chamomile tea. The SWASV experiments did not reveal significant influence on the signals of Cd 2+ and Pb 2+ , for a range of organic and inorganic interferents. Therefore, the developed work fills a gap in the development of electroanalytical devices towards metals detection in complex matrices.