Preparation and Application of a Fast, Naked-Eye, Highly Selective, and Highly Sensitive Fluorescent Probe of Schiff Base for Detection of Cu 2+

: A fluorescent probe, N (cid:48) -((3-methyl-5-oxo-1-phenyl-4, 5-dihydro-1H-pyrazol-4-yl) methylene)-2- oxo-2 H -chromene-3-carbohydrazide (MPMC), was synthesized and characterized. Characterizations of the synthetic MPMC were conducted via proton nuclear magnetic resonance ( 1 HNMR) spectroscopy and carbon-13 nuclear magnetic resonance spectroscopy ( 13 C NMR). The ﬂuorescence emission behaviors of the MPMC probe towards diverse metal ions were detected, and the probe exhibited high sensitivity and selectivity towards Cu 2+ over other metal ions via the quenching of its ﬂuorescence. Furthermore, the existence of other metal actions made no apparent difference to the ﬂuorescence intensity of the MPMC-Cu 2+ system; that is, MPMC displayed a good anti-interference ability. Job’s plot of the MPMC and copper ions indicated that the detection limit was 10.23 nM (R 2 = 0.9612) for the assayed actions, with a stoichiometric ratio of 1:1 for MPMC and Cu 2+ . Additionally, the color of the MPMC probe solution changed from nearly colorless to yellow in the presence of Cu 2+ in visible light, and the color change could be observed by the naked eye. Similarly, the color resolved from bright yellow into blue in ultraviolet light. Moreover, reusability studies indicated that the MPMC probe was reusable. The pH effect of the MPMC probe on Cu 2+ had a broad range of pH detection, i.e., from 4.0 to 11.0. The response time of the MPMC probe for determining Cu 2+ was within 1 min. The recognition of Cu 2+ via MPMC performed on pre-treated paper under sunlight and UV light both had a distinct colour change. Thus, the solid-state method for detecting Cu 2+ with the naked eye was both economical and convenient.


Introduction
Copper (II) ion is one of the essential transition metal ions that play a crucial role in many key physiological processes in living organisms, including electron transport oxidoreductases, the production of hemocyanin, blue copper protein, cytochrome C oxidase, lactase, ascorbate oxidase, superoxide dismutase, skin pigmentation, and connective tissue repair [1][2][3].However, excessive amounts of Cu 2+ can lead to a variety of diseases, such as induction of cell death, Parkinson's, Alzheimer's, and prion diseases [4][5][6].In addition, the widespread use of Cu 2+ in the electronic and electrical industries makes it an environmental pollutant.Especially in recent years, the operations of ore mining along with metals extraction in nature are growing, which brings about severe heavy metals pollution (copper, cadmium, etc.) in the atmosphere, water resources, and soil.These heavy metals are incapable of being degraded by organisms; instead, they can be enriched thousands of times via the biomagnification of food chain; then, they intrude into the human body, making them a serious health hazard [7,8].Consequently, the development of a highly sensitive and selective Cu 2+ assay for the efficient detection of Cu 2+ in aqueous solutions or biological systems is of great importance for biology, clinical medicine, chemistry, and environmental sciences.
Nowadays, various detection technologies have been developed to test Cu 2+ , such as chemiluminescence [9], electro-chemistry [10], colorimetry [11], atomic absorption spectrometry [12], and inductively coupled plasma mass spectrometry [13].However, these detection strategies have the drawbacks of low sensitivity and cumbersome operation.In recent years, fluorescent molecular probes have been rapidly developing in molecular recognition because of their excellent recognition properties, high selectivity, and sensitivity, being accessible in site detection and real-time imaging.The "host-guest" pattern is a fundamental structural model of fluorescent probes, including, chiefly, fluorophore and receptor, connected by covalent bonding through a spacer.Recognition units that possess different recognition functions serve to grasp and recognize test samples.Meanwhile, for the difference of molecular structure before and after grasping, fluorophore's fluorescence property displays different fluorescence signals (wavelength or intensity variation), so as to achieve the purpose of recognition and detection [14][15][16][17].Actually, most fluorescent probes that identify Cu 2+ are restricted in their applications for the poor selectivity, short wavelength emission, interference from autofluorescence, the need for a high organic phase detection environment, and the presence of many possible interfering agents.
In the field of analytical chemistry, as a conjugated double bond, Schiff bases are recommended for the detection and identification of metal ions.Additionally, Schiff bases are applied to the quantitative analysis of certain ions by means of chromatography, fluorescence analysis, and photometric analysis.Meanwhile, for the space-turning effect of -C=N-in the molecular structure, Schiff bases and their complexes tend to coordinate easily.In addition, lone-pair electrons of the integral structure are more abundant, such that the coordination sites also become more abundant, which indicates that Schiff bases have good, coordinated properties.At this stage, Schiff bases make coordination with metallic cations easier, especially the heavy metal ions.Thus, Schiff bases are good ligands for metal ions [19][20][21][22].

Synthesis of Edaravone
A total of 13.0 g and 0.1 mol of acetyl ethyl acetate was first mixed with 5 mL of 60% ethanol solution while stirring.Next, a solution consisting of phenylhydrazine (10.8 g, 0.1 mol) and absolute ethanol was added dropwise at 45 • C in approximately 30 min.After dripping, the reaction proceeded at 45 • C for 20 min.Thereafter, the reaction solution was cooled to room temperature, supplemented with 1 mL of concentrated hydrochloric acid, reacting at 45 • C for another 2 h.The following is the addition of 10% sodium hydroxide solution, dropwise, to adjust the pH to be 7.0.Then, the solution was added by 20 mL of water and stirred at room temperature for 1 h, followed by filtering to obtain light yellow crystals.Finally, crystals were washed with cold absolute ethanol and heated to reflux in ethyl acetate and absolute ethanol solution (with a volume ratio of 2:1) to obtain white and pure edaravone (compound 1).The synthetic route of edaravone is shown in Scheme 2.

Synthesis of Edaravone
A total of 13.0 g and 0.1 mol of acetyl ethyl acetate was first mixed with 5 mL of 60% ethanol solution while stirring.Next, a solution consisting of phenylhydrazine (10.8 g, 0.1 mol) and absolute ethanol was added dropwise at 45 °C in approximately 30 min.After dripping, the reaction proceeded at 45 °C for 20 min.Thereafter, the reaction solution was cooled to room temperature, supplemented with 1 mL of concentrated hydrochloric acid, reacting at 45 °C for another 2 h.The following is the addition of 10% sodium hydroxide solution, dropwise, to adjust the pH to be 7.0.Then, the solution was added by 20 mL of water and stirred at room temperature for 1 h, followed by filtering to obtain light yellow crystals.Finally, crystals were washed with cold absolute ethanol and heated to reflux in ethyl acetate and absolute ethanol solution (with a volume ratio of 2:1) to obtain white and pure edaravone (compound 1).The synthetic route of edaravone is shown in Scheme 2.

Synthesis of Edaravone Aldehyde Derivative
Edaravone (8.70 g, 0.05 mol) was dissolved in 1 mL of N,N-dimethylformamide under stirring; then, 10.1 mL of phosphorus oxychloride was introduced slowly in drops by an external ice bath.The reaction mixture was then heated to reflux for 1.5 h.After cooling, it was introduced into 200 mL of ice water, then oscillated, stood still, and filtered; thus, crude edaravone aldehyde derivative was obtained.Subsequently, the crude product was treated by absolute ethanol washing, heated to reflux in ethanol, followed by drying to gain the pure edaravone aldehyde derivative (compound 2).The synthetic route of edaravone aldehyde derivative is shown in Scheme 3.

Synthesis of Edaravone
A total of 13.0 g and 0.1 mol of acetyl ethyl acetate was first mixed with 5 mL of 60% ethanol solution while stirring.Next, a solution consisting of phenylhydrazine (10.8 g, 0.1 mol) and absolute ethanol was added dropwise at 45 °C in approximately 30 min.After dripping, the reaction proceeded at 45 °C for 20 min.Thereafter, the reaction solution was cooled to room temperature, supplemented with 1 mL of concentrated hydrochloric acid, reacting at 45 °C for another 2 h.The following is the addition of 10% sodium hydroxide solution, dropwise, to adjust the pH to be 7.0.Then, the solution was added by 20 mL of water and stirred at room temperature for 1 h, followed by filtering to obtain light yellow crystals.Finally, crystals were washed with cold absolute ethanol and heated to reflux in ethyl acetate and absolute ethanol solution (with a volume ratio of 2:1) to obtain white and pure edaravone (compound 1).The synthetic route of edaravone is shown in Scheme 2.

Synthesis of Edaravone Aldehyde Derivative
Edaravone (8.70 g, 0.05 mol) was dissolved in 1 mL of N,N-dimethylformamide under stirring; then, 10.1 mL of phosphorus oxychloride was introduced slowly in drops by an external ice bath.The reaction mixture was then heated to reflux for 1.5 h.After cooling, it was introduced into 200 mL of ice water, then oscillated, stood still, and filtered; thus, crude edaravone aldehyde derivative was obtained.Subsequently, the crude product was treated by absolute ethanol washing, heated to reflux in ethanol, followed by drying to gain the pure edaravone aldehyde derivative (compound 2).The synthetic route of edaravone aldehyde derivative is shown in Scheme 3.

Synthesis of Edaravone
A total of 13.0 g and 0.1 mol of acetyl ethyl acetate was first mixed with 5 mL of 60% ethanol solution while stirring.Next, a solution consisting of phenylhydrazine (10.8 g, 0.1 mol) and absolute ethanol was added dropwise at 45 °C in approximately 30 min.After dripping, the reaction proceeded at 45 °C for 20 min.Thereafter, the reaction solution was cooled to room temperature, supplemented with 1 mL of concentrated hydrochloric acid, reacting at 45 °C for another 2 h.The following is the addition of 10% sodium hydroxide solution, dropwise, to adjust the pH to be 7.0.Then, the solution was added by 20 mL of water and stirred at room temperature for 1 h, followed by filtering to obtain light yellow crystals.Finally, crystals were washed with cold absolute ethanol and heated to reflux in ethyl acetate and absolute ethanol solution (with a volume ratio of 2:1) to obtain white and pure edaravone (compound 1).The synthetic route of edaravone is shown in Scheme 2.

Synthesis of Edaravone Aldehyde Derivative
Edaravone (8.70 g, 0.05 mol) was dissolved in 1 mL of N,N-dimethylformamide under stirring; then, 10.1 mL of phosphorus oxychloride was introduced slowly in drops by an external ice bath.The reaction mixture was then heated to reflux for 1.5 h.After cooling, it was introduced into 200 mL of ice water, then oscillated, stood still, and filtered; thus, crude edaravone aldehyde derivative was obtained.Subsequently, the crude product was treated by absolute ethanol washing, heated to reflux in ethanol, followed by drying to gain the pure edaravone aldehyde derivative (compound 2).The synthetic route of edaravone aldehyde derivative is shown in Scheme 3.

Synthesis of Ethyl Coumarin-3-Carboxylate
Salicylaldehyde (4.90 g, 0.04 mol), diethyl malonate (7.25 g, 0.045 mol), and 20 mL of absolute ethanol were successively added and thoroughly mixed, and then 0.5 mL of hexahydropyridine and two drops of acetic acid were dripped.In succession, the reaction mixture was then heated to reflux for 2 h while stirring.After being cooled to dilution crystallization, filtering was adopted to obtain the crude product, which was then washed with ethanol several times and dried to obtain the pure ethyl coumarin-3-carboxylate (compound 3).The synthetic route of ethyl coumarin-3-carboxylate is shown in Scheme 4.
Salicylaldehyde (4.90 g, 0.04 mol), diethyl malonate (7.25 g, 0.045 mol), and 20 mL of absolute ethanol were successively added and thoroughly mixed, and then 0.5 mL of hexahydropyridine and two drops of acetic acid were dripped.In succession, the reaction mixture was then heated to reflux for 2 h while stirring.After being cooled to dilution crystallization, filtering was adopted to obtain the crude product, which was then washed with ethanol several times and dried to obtain the pure ethyl coumarin-3-carboxylate (compound 3).The synthetic route of ethyl coumarin-3-carboxylate is shown in Scheme 4. Scheme 4. Synthesis of ethyl coumarin-3-carboxylate.

Synthesis of Coumarin-3-Carbohydrazide
In the presence of coumarin-3-carboxylic acid ethyl ester (2.00 g, 0.01 mol) and 50 mL of anhydrous ethanol, five drops of hydrazine hydrate (0.01 mol) were then added.The reaction mixture was heated to reflux for 10 h under agitation.Then, crystals of coumarin-3-carbohydrazide were formed while being cold.After filtering, yellow crystals were obtained.The yellow crystals were washed by absolute ethanol and dissolved with reflux in absolute ethanol.Finally, bright yellow crystals, which were the pure coumarin-3-carbohydrazide (compound 4), were produced via the dryness.The synthetic route of coumarin-3-carbohydrazide is shown in Scheme 5.

Synthesis of Coumarin-3-Carbohydrazide
In the presence of coumarin-3-carboxylic acid ethyl ester (2.00 g, 0.01 mol) and 50 mL of anhydrous ethanol, five drops of hydrazine hydrate (0.01 mol) were then added.The reaction mixture was heated to reflux for 10 h under agitation.Then, crystals of coumarin-3-carbohydrazide were formed while being cold.After filtering, yellow crystals were obtained.The yellow crystals were washed by absolute ethanol and dissolved with reflux in absolute ethanol.Finally, bright yellow crystals, which were the pure coumarin-3carbohydrazide (compound 4), were produced via the dryness.The synthetic route of coumarin-3-carbohydrazide is shown in Scheme 5.
absolute ethanol were successively added and thoroughly mixed, and then 0.5 mL of hexahydropyridine and two drops of acetic acid were dripped.In succession, the reaction mixture was then heated to reflux for 2 h while stirring.After being cooled to dilution crystallization, filtering was adopted to obtain the crude product, which was then washed with ethanol several times and dried to obtain the pure ethyl coumarin-3-carboxylate (compound 3).The synthetic route of ethyl coumarin-3-carboxylate is shown in Scheme 4.

Synthesis of Coumarin-3-Carbohydrazide
In the presence of coumarin-3-carboxylic acid ethyl ester (2.00 g, 0.01 mol) and 50 mL of anhydrous ethanol, five drops of hydrazine hydrate (0.01 mol) were then added.The reaction mixture was heated to reflux for 10 h under agitation.Then, crystals of coumarin-3-carbohydrazide were formed while being cold.After filtering, yellow crystals were obtained.The yellow crystals were washed by absolute ethanol and dissolved with reflux in absolute ethanol.Finally, bright yellow crystals, which were the pure coumarin-3-carbohydrazide (compound 4), were produced via the dryness.The synthetic route of coumarin-3-carbohydrazide is shown in Scheme 5.

General Procedure for the Spectrum Measurement
Stock solutions of MPMC (1 mM) were prepared in ethanol.The metal ions stock solutions (1 mM) were prepared with the nitrate or chloride salts (Cu 2+ , Pb 2+ , Fe 3+ , Fe 2+ , Mg 2+ , Ni 2+ , Cd 2+ , Co 2+ , Mn 2+ , Ca 2+ , Ba 2+ , Na + , K + , Zn 2+ and Ag + ) in deionized water.Absorption and emission spectra were obtained at room temperature using PBS solution (pH 7.5) in MPMC (10 µM) with different concentrations of analyzer.Fluorescence spectra were recorded at an excitation wavelength of 358 nm.The total concentration of Cu 2+ and MPMC was kept constantly (2.0 mM).The fluorescence intensity of MPMC was then recorded by varying the molar ratio of MPMC to Cu 2+ .The selectivity of MPMC towards Cu 2+ was tested by comparing other metal ions.MPMC (1 mM) was treated with Cu 2+ (1 mM) and other metal ions (10 mM) for 10 min and the fluorescence intensity of the mixtures was recorded.For reproducibility testing, Cu 2+ (1.0 mM) was incubated with MPMC aqueous solution (1.0 mM), and the fluorescence of MPMC was quenched.The fluorescence intensity of MPMC (1.0 mM) and MPMC-Cu 2+ ensemble (1.0 mM) was determined in a series of buffers (pH 1.0 to 14.0).

Fluorescence Spectra of the MPMC Probe for Selectivity and Anti-Interference Detection
The fluorescence emission behaviors of the MPMC probe towards diverse metal ions were detected in ethanol (EtOH)/H 2 O (v/v = 1/1) solution, respectively.Fluorescence intensities of diverse metal ions added to MPMC were detected as the wavelength variation.The fluorescence emission spectrums are illustrated in Figure 1a.As shown in Figure 1a, when excitation wavelength 358 nm was given to the MPMC probe, a fluorescence emission band at 548 nm generated.Then fluorescence quenching followed along with the insertion of Cu 2+ (1.0 equiv), with the emission minimum being at 548 nm.Conversely, the fluorescence emission spectrums of MPMC were affected little by introducing other metal ions, in addition to a weak quenching of nickel ions.
were detected in ethanol (EtOH)/H2O (v/v = 1/1) solution, respectively.Fluorescence intensities of diverse metal ions added to MPMC were detected as the wavelength variation.The fluorescence emission spectrums are illustrated in Figure 1a.As shown in Figure 1a, when excitation wavelength 358 nm was given to the MPMC probe, a fluorescence emission band at 548 nm generated.Then fluorescence quenching followed along with the insertion of Cu 2+ (1.0 equiv), with the emission minimum being at 548 nm.Conversely, the fluorescence emission spectrums of MPMC were affected little by introducing other metal ions, in addition to a weak quenching of nickel ions.
Furthermore, in view of potential interference of other metal ions in practical applications, sorts of metal ions were added successively into the MPMC-Cu 2+ system, and the influences on fluorescence selectivity were investigated.
A total of 100 μM of competitive metal ions (Pb 2+ , Fe 3+ , Fe 2+ , Mg 2+ , Ni 2+ , Cd 2+ , Co 2+ , Mn 2+ , Ca 2+ , Ba 2+ , Na + , K + , Zn 2+ , Ag + ), along with 10 μM MPMC in EtOH-H2O (v/v = 1/1), were prepared and determined to access the fluorescence intensities.As Figure 1b illustrates, a fluorescence emission band at 548 nm was generated again.Thereafter, with the addition of 10 μM Cu 2+ , the fluorescence quenching took place.The existence of other metal actions made no difference on the fluorescence intensity apparently.In general, the MPMC probe has the characteristic of selectively recognizing Cu 2+ , displaying a good antiinterference ability.Furthermore, in view of potential interference of other metal ions in practical applications, sorts of metal ions were added successively into the MPMC-Cu 2+ system, and the influences on fluorescence selectivity were investigated.

Titration Experiment of the MPMC Probe to Cu 2+
Colorimetric experiments were conducted to study the specificity of the MPMC probe towards Cu 2+ .As Figure 2a shows, while adding Cu 2+ to MPMC dissolved in EtOH/H 2 O (v/v = 1/1), the color of solution passed from colorless to yellow in seconds.The result shows that the MPMC probe can realize the colorimetric detection of Cu 2+ with a detection limit 10 µM.In the same way, the fluorescence color turned from colorless to yellow under 365 nm ultraviolet (UV) light, shown in Figure 2b.The applied results clearly demonstrate that the MPMC probe could have an application in the qualitative and quantitative detection of Cu 2+ in the form of color change and spectrum signals multiplication.
probe towards Cu 2+ .As Figure 2a shows, while adding Cu 2+ to MPMC dissolved in EtOH/H2O (v/v = 1/1), the color of solution passed from colorless to yellow in seconds.The result shows that the MPMC probe can realize the colorimetric detection of Cu 2+ with a detection limit 10 μM.In the same way, the fluorescence color turned from colorless to yellow under 365 nm ultraviolet (UV) light, shown in Figure 2b.The applied results clearly demonstrate that the MPMC probe could have an application in the qualitative and quantitative detection of Cu 2+ in the form of color change and spectrum signals multiplication.As the concentration variation of Cu 2+ , fluorescence titration experiments were performed to explore the sensitivity of the MPMC probe to Cu 2+ .As illustrated in Figure 3, with the Cu 2+ concentration rising, the fluorescence intensity of the MPMC probe reflected a continued decrease.The linear equation for copper (II) ions is Y = −728.21X+ 1388.2, and the correlated coefficient is 0.9612.Based on the formula L = 3σ/Ka, the minimum detectable concentration of the probe for copper (II) ions was calculated as 10.23 nM [31].Compared with other fluorescent probes for the detection of Cu 2+ listed in Table 1, the MPMC probe prepared in this paper has a lower detection limit.As the concentration variation of Cu 2+ , fluorescence titration experiments were performed to explore the sensitivity of the MPMC probe to Cu 2+ .As illustrated in Figure 3, with the Cu 2+ concentration rising, the fluorescence intensity of the MPMC probe reflected a continued decrease.The linear equation for copper (II) ions is Y = −728.21X+ 1388.2, and the correlated coefficient is 0.9612.Based on the formula L = 3σ/Ka, the minimum detectable concentration of the probe for copper (II) ions was calculated as 10.23 nM [31].Compared with other fluorescent probes for the detection of Cu 2+ listed in Table 1, the MPMC probe prepared in this paper has a lower detection limit.
probe towards Cu 2+ .As Figure 2a shows, while adding Cu 2+ to MPMC dissolved in EtOH/H2O (v/v = 1/1), the color of solution passed from colorless to yellow in seconds.The result shows that the MPMC probe can realize the colorimetric detection of Cu 2+ with a detection limit 10 μM.In the same way, the fluorescence color turned from colorless to yellow under 365 nm ultraviolet (UV) light, shown in Figure 2b.The applied results clearly demonstrate that the MPMC probe could have an application in the qualitative and quantitative detection of Cu 2+ in the form of color change and spectrum signals multiplication.As the concentration variation of Cu 2+ , fluorescence titration experiments were performed to explore the sensitivity of the MPMC probe to Cu 2+ .As illustrated in Figure 3, with the Cu 2+ concentration rising, the fluorescence intensity of the MPMC probe reflected a continued decrease.The linear equation for copper (II) ions is Y = −728.21X+ 1388.2, and the correlated coefficient is 0.9612.Based on the formula L = 3σ/Ka, the minimum detectable concentration of the probe for copper (II) ions was calculated as 10.23 nM [31].Compared with other fluorescent probes for the detection of Cu 2+ listed in Table 1, the MPMC probe prepared in this paper has a lower detection limit.

Study of EDTA Effect of MPMC Probe on Cu 2+
To fully investigate the response between MPMC probe and Cu 2+ , the procedures involved the addition of ethylene diamine tetraacetate acid (EDTA) to MPMC-Cu 2+

Study of EDTA Effect of MPMC Probe on Cu 2+
To fully investigate the response between MPMC probe and Cu 2+ , the procedures involved the addition of ethylene diamine tetraacetate acid (EDTA) to MPMC-Cu 2+

Study of EDTA Effect of MPMC Probe on Cu 2+
To fully investigate the response between MPMC probe and Cu 2+ , the procedures involved the addition of ethylene diamine tetraacetate acid (EDTA) to MPMC-Cu 2+

Study of EDTA Effect of MPMC Probe on Cu 2+
To fully investigate the response between MPMC probe and Cu 2+ , the procedures involved the addition of ethylene diamine tetraacetate acid (EDTA) to MPMC-Cu 2+

Study of EDTA Effect of MPMC Probe on Cu 2+
To fully investigate the response between MPMC probe and Cu 2+ , the procedures involved the addition of ethylene diamine tetraacetate acid (EDTA) to MPMC-Cu 2+ complex, Water/DMF (9.9/0.

Study of EDTA Effect of MPMC Probe on Cu 2+
To fully investigate the response between MPMC probe and Cu 2+ , the procedures involved the addition of ethylene diamine tetraacetate acid (EDTA) to MPMC-Cu 2+  To fully investigate the response between MPMC probe and Cu 2+ , the procedures involved the addition of ethylene diamine tetraacetate acid (EDTA) to MPMC-Cu 2+  To fully investigate the response between MPMC probe and Cu 2+ , the procedures involved the addition of ethylene diamine tetraacetate acid (EDTA) to MPMC-Cu 2+ complex, and the detection of fluorescence intensity was performed.As shown in Figure 4, the fluorescence intensity was recovered with the additional amount of EDTA added into the MPMC-Cu 2+ complex.It suggests that the complexation ability of EDTA to Cu 2+ is stronger than that of the MPMC probe.EDTA seized the Cu 2+ bound to the MPMC probe, leading to the recovery of fluorescence.Namely, the fluorescence recognition between MPMC probe and Cu 2+ is reversible [41].Then, the fluorescence quenching still occurred after the sustainable addition of Cu 2+ .Similarly, the fluorescence intensity recovered again after the addition of EDTA.The results indicate that the MPMC probe is reusable.

Study of pH Effect of MPMC Probe to Cu 2+
The MPMC probe is required not only for highly sensitive and selective performance, but also for its good sensing ability at different acidities in practical applications.The sensing ability was detected by adjusting the pH of EtOH-PBS (phosphate buffer solution) from 1.0 to 14.0.As Figure 5 illustrates, with a range 4.0-11.0 of pH variation, the fluorescence intensity of the MPMC probe was maintained constantly.It then dropped dramatically in a range 5.0-8.0 pH while adding Cu 2+ to the MPMC solution.At a low pH range, the fluorescence intensity exhibited no variance; it was probably caused by hydrolysis of Cu 2+ under acidic conditions, which inhibited the formation of the MPMC-Cu 2+ complex 30 .Thus, the prepared MPMC probe acted as a fluorescent pH sensor, suitable for a broad range of pH detection (4.0 to 11.0), especially from 5.0 to 8.0.
Chemosensors 2023, 11, x FOR PEER REVIEW 9 and the detection of fluorescence intensity was performed.As shown in Figure 4, th orescence intensity was recovered with the additional amount of EDTA added int MPMC-Cu 2+ complex.It suggests that the complexation ability of EDTA to Cu 2+ is stro than that of the MPMC probe.EDTA seized the Cu 2+ bound to the MPMC probe, lea to the recovery of fluorescence.Namely, the fluorescence recognition between M probe and Cu 2+ is reversible [41].Then, the fluorescence quenching still occurred afte sustainable addition of Cu 2+ .Similarly, the fluorescence intensity recovered again afte addition of EDTA.The results indicate that the MPMC probe is reusable.

Study of pH Effect of MPMC Probe to Cu 2+
The MPMC probe is required not only for highly sensitive and selective perform but also for its good sensing ability at different acidities in practical applications.The ing ability was detected by adjusting the pH of EtOH-PBS (phosphate buffer solu from 1.0 to 14.0.As Figure 5

Study of the Response Time of MPMC Probe to Cu 2+
The response time of the MPMC probe in determining copper (II) ions was obtained.The variation of the fluorescence intensity at 548 nm with the reaction time of the MPMC probe along with Cu 2+ is shown in Figure 6.After the addition of copper (II) ions, the fluorescence intensity of the MPMC probe declined markedly within 1 min then achieved an equilibrium after 2 min.

Study of the Response Time of MPMC Probe to Cu 2+
The response time of the MPMC probe in determining copper (II) ions was obtained.The variation of the fluorescence intensity at 548 nm with the reaction time of the MPMC probe along with Cu 2+ is shown in Figure 6.After the addition of copper (II) ions, the fluorescence intensity of the MPMC probe declined markedly within 1 min then achieved an equilibrium after 2 min.

Study of the Response Time of MPMC Probe to Cu 2+
The response time of the MPMC probe in determining copper (II) ions was obtained.The variation of the fluorescence intensity at 548 nm with the reaction time of the MPMC probe along with Cu 2+ is shown in Figure 6.After the addition of copper (II) ions, the fluorescence intensity of the MPMC probe declined markedly within 1 min then achieved an equilibrium after 2 min.

Contact Mode Detection between MPMC Probe and Cu 2+
To explore the binding ratio of the MPMC probe to Cu 2+ , different ratios of the MPMC probe to Cu 2+ (1:9, 2:8, 3:7, 4:6, 5:5, 6:4, 7:3, 8:2, 9:1) were prepared.As the molar fraction changed, the fluorescence characteristics were analyzed, and Job's plot curve is illustrated in Figure 7.With the molar fraction varying from 1:9 to 5:5, the fluorescence intensity decreased first, while the fluorescence intensity was almost unchanged in the range from 5:5 to 9:1.The molar fraction corresponding to the minimum fluorescence intensity of the MPMC probe appeared at 0.5, indicating that MPMC-Cu 2+ complex was formed by 1:1.The possible binding mechanism of MPMC to Cu 2+ -induced fluorescence changes is shown in Figure 8.The possible binding mechanism of MPMC to Cu 2+ -induced fluorescence changes is shown in Figure 8.

The Test Paper for Cu 2+ Ions
To study the multifunction applications of the solid-state MPMC probe for its high efficiency and simplicity, the recognition of Cu 2+ by MPMC was performed on pre-treated paper.Filter papers were steeped in MPMC probe dissolved in an ethanol-saturated solution (1.0 × 10 −3 mol/L) for a few seconds to obtain test strips.Afterward, solid-state experiments were carried out by drying test strips in air and treating with an aqueous solution of Cu 2+ (1.0 × 10 −3 mol/L).Under sunlight, test strips before and after solid-state experiments presented white and yellow colors, respectively, as seen in Figure 9.In the same way, the color became light yellow and blue under 365 nm UV light, respectively [42].Thus, the solid-state method for detecting Cu 2+ with the naked eye was both economical and convenient.

The Test Paper for Cu 2+ Ions
To study the multifunction applications of the solid-state MPMC probe for its high efficiency and simplicity, the recognition of Cu 2+ by MPMC was performed on pre-treated paper.Filter papers were steeped in MPMC probe dissolved in an ethanol-saturated solution (1.0 × 10 −3 mol/L) for a few seconds to obtain test strips.Afterward, solid-state experiments were carried out by drying test strips in air treating with an aqueous solution of Cu 2+ (1.0 × 10 −3 mol/L).Under sunlight, test strips before and after solid-state experiments presented white and yellow colors, respectively, as seen in Figure 9.In the same way, the color became light yellow and blue under 365 nm UV light, respectively [42].Thus, the solid-state method for detecting Cu 2+ with the naked eye was both economical and convenient.

Conclusions
A fluorescent probe with highly selective and sensitive performance based on coumarin and pyrazole Schiff base has been synthesized.The fluorescence emission behaviors of the MPMC probe towards diverse metal ions were detected, and the probe exhibited high sensitivity and selectivity towards Cu 2+ over other metal ions via the quenching of its fluorescence.Furthermore, the existence of other metal actions made no apparent difference to the fluorescence intensity of the MPMC-Cu 2+ system; that is, MPMC displayed a good anti-interference ability.Job's plot of MPMC and copper ions indicated that the detection limit was 10.23 nM (R 2 = 0.9612) for the assayed actions, with a stoichiometric ratio of 1:1 for MPMC and Cu 2+ .Additionally, the color of the MPMC probe solution changed from nearly colorless to yellow in the presence of Cu 2+ in visible light, and the color change could be observed by the naked eye.Similarly, the color resolved from bright yellow into blue in ultraviolet light.Moreover, the MPMC probe was reusable.The pH effect of the MPMC probe on Cu 2+ had a broad range of pH detection (4.0 to 11.0, especially from 5.0 to 8.0).The response time of the MPMC probe for determining Cu 2+ was within 1 min.The recognition of Cu 2+ by MPMC performed on pre-treated paper under sunlight and UV light both had a distinct colour change.In conclusion, the MPMC probe could be applied to recognize Cu 2+ in the environment; moreover, applications in biological systems may be achieved in future.

Conclusions
A fluorescent probe with highly selective and sensitive performance based on coumarin and pyrazole Schiff base has been synthesized.The fluorescence emission behaviors of the MPMC probe towards diverse metal ions were detected, and the probe exhibited high sensitivity and selectivity towards Cu 2+ over other metal ions via the quenching of its fluorescence.Furthermore, the existence of other metal actions made no apparent difference to the fluorescence intensity of the MPMC-Cu 2+ system; that is, MPMC displayed a good anti-interference ability.Job's plot of MPMC and copper ions indicated that the detection limit was 10.23 nM (R 2 = 0.9612) for the assayed actions, with a stoichiometric ratio of 1:1 for MPMC and Cu 2+ .Additionally, the color of the MPMC probe solution changed from nearly colorless to yellow in the presence of Cu 2+ in visible light, and the color change could be observed by the naked eye.Similarly, the color resolved from bright yellow into blue in ultraviolet light.Moreover, the MPMC probe was reusable.The pH effect of the MPMC probe on Cu 2+ had a broad range of pH detection (4.0 to 11.0, especially from 5.0 to 8.0).The response time of the MPMC probe for determining Cu 2+ was within 1 min.The recognition of Cu 2+ by MPMC performed on pre-treated paper under sunlight and UV light both had a distinct colour change.In conclusion, the MPMC probe could be applied to recognize Cu 2+ in the environment; moreover, applications in biological systems may be achieved in future.
illustrates, with a range 4.0-11.0 of pH variation, the flu cence intensity of the MPMC probe was maintained constantly.It then dropped dra cally in a range 5.0-8.0 pH while adding Cu 2+ to the MPMC solution.At a low pH ra the fluorescence intensity exhibited no variance; it was probably caused by hydroly Cu 2+ under acidic conditions, which inhibited the formation of the MPMC-Cu 2+ comp Thus, the prepared MPMC probe acted as a fluorescent pH sensor, suitable for a b range of pH detection (4.0 to 11.0), especially from 5.0 to 8.0.

Figure 8 .
Figure 8.The proposed sensing mechanism of MPMC to Cu 2+ in the system.

Figure 8 .
Figure 8.The proposed sensing mechanism of MPMC to Cu 2+ in the system.

Chemosensors 2023 , 15 Figure 9 .
Figure 9. Photographs showing the color changes of the MPMC probe (1.0 mM) before and after addition of Cu 2+ (1.0 mM) under (a) sunlight and (b) 365 nm UV light.

Figure 9 .
Figure 9. Photographs showing the color changes of the MPMC probe (1.0 mM) before and after addition of Cu 2+ (1.0 mM) under (a) sunlight and (b) 365 nm UV light.

Table 1 .
Performance of MPMC compared with available Cu 2+ probes.

Table 1 .
Performance of MPMC compared with available Cu 2+ probes.