Mixed Oxime-Functionalized IL/16- s -16 Gemini Surfactants System: Physicochemical Study and Structural Transitions in the Presence of Promethazine as a Potential Chiral Pollutant

: The increasing concern about chiral pharmaceutical pollutants is connected to environmental contamination causing both chronic and acute harmful effects on living organisms. The design and application of sustainable surfactants in the remediation of polluted sites require knowledge of partitioning between surfactants and potential pollutants. The interfacial and thermodynamic properties of two gemini surfactants, namely, alkanediyi- α , ω - bis (dimethylhexadecyl ammonium bromide) (16- s -16, where s = 10, 12), were studied in the presence of the inherently biodegradable oxime-functionalized ionic liquid (IL) 4-((hydroxyimino)methyl)-1-(2-(octylamino)-2-oxoethyl)pyridin-1-ium bromide (4-PyC8) in an aqueous solution using surface tension, conductivity, ﬂuorescence, FTIR and 1 H NMR spectroscopic techniques. The conductivity, surface tension and ﬂuorescence measurements indicated that the presence of the IL 4-PyC8 resulted in decreasing CMC and facilitated the aggregation process. The various thermodynamic parameters, interfacial properties, aggregation number and Stern–Volmer constant were also evaluated. The IL 4-PyC8-gemini interactions were studied using DLS, FTIR and NMR spectroscopic techniques. The hydrodynamic diameter of the gemini aggregates in the presence of promethazine (PMZ) as a potential chiral pollutant and the IL 4-PyC8 underwent a transition when the drug was added, from large aggregates (270 nm) to small micelles, which supported the gemini:IL 4-PyC8:promethazine interaction. The structural transitions in the presence of promethazine may be used for designing systems that are responsive to changes in size and shape of the aggregates as an analytical signal for selective detection and binding pollutants. surfactants , -16, where s 12) with functionalized IL 4-((hydroxyimino) methyl)-1-(2-(octylamino)-2-oxoethyl) pyridin-1-iumbromide (4-PyC8), were studied using surface tension, conductivity, ﬂuorescence, FTIR and 1 H NMR techniques. The effect of 4-PyC8 on the aggregation behavior, i.e., critical micelle concentration (CMC), surface excess concentration ( Γ max ), surface pressure at CMC ( π cmc ) and minimum area per molecule ( A min ), were determined using the surface tension method. The thermodynamic parameters, i.e., the standard Gibbs free energy of aggregation ( ∆ G om ), Gibbs energy of adsorption ( ∆ G ◦ ads ) , Gibbs energy of transfer ( ∆ G ◦ trans ), Gibbs energy of micellization per alkyl tail ( ∆ G ◦ tail ) and air–water interface ( ∆ G smin ), were evaluated using a conductrometric technique. The CMC, aggregation number ( N agg ) and Stern-Volmer constant ( K sv ) were also determined via a ﬂuorescence method using pyrene (Pyr) and 1-pyrene carboxaldehyde (PyrCHO) as the probes. FTIR and NMR techniques were successfully used to study the IL 4-PyC8:gemini interactions. The sizes of the gemini aggregates in the presence of PMZ and IL 4-PyC8 were studied using dynamic light scattering (DLS).


Introduction
The increasing concern about chiral pharmaceutical pollutants is connected to environmental contamination causing both chronic and acute harmful effects on living organisms. It is a problem of direct importance to detect chiral compounds [1], including chiral pollutants of different natures [2]. Using surfactants for increasing analytical signals and, consequently, reducing the detection concentration of the pollutants is one of the attractive strategies in chemical analysis [3,4] since it may ensure selective binding of one of the components, providing more reliable detection in the cases when the structurally similar compounds are present in the mixture. For example, dimeric (gemini) surfactants were reported to exhibit selectivity toward the binding of calixarenes modified with different surfactants alkanediyi-α,ω-bis (dimethylhexadecyl ammonium bromide) (16-s-16, where s = 10, 12) (Scheme 1) with functionalized IL 4-((hydroxyimino) methyl)-1-(2-(octylamino)-2oxoethyl) pyridin-1-iumbromide (4-PyC8), were studied using surface tension, conductivity, fluorescence, FTIR and 1 H NMR techniques. The effect of 4-PyC8 on the aggregation behavior, i.e., critical micelle concentration (CMC), surface excess concentration (Γ max ), surface pressure at CMC (π cmc ) and minimum area per molecule (A min ), were determined using the surface tension method. The thermodynamic parameters, i.e., the standard Gibbs free energy of aggregation (∆G o m ), Gibbs energy of adsorption (∆G • ads ) , Gibbs energy of transfer (∆G • trans ), Gibbs energy of micellization per alkyl tail (∆G • tail ) and air-water interface (∆G s min ), were evaluated using a conductrometric technique. The CMC, aggregation number (N agg ) and Stern-Volmer constant (K sv ) were also determined via a fluorescence method using pyrene (Pyr) and 1-pyrene carboxaldehyde (PyrCHO) as the probes. FTIR and NMR techniques were successfully used to study the IL 4-PyC8:gemini interactions. The sizes of the gemini aggregates in the presence of PMZ and IL 4-PyC8 were studied using dynamic light scattering (DLS).

Conductivity
A digital conductivity meter (Systronics, Type 304) equipped with a conductivity cell of cell constant 1.01 cm −1 was used to measure the conductance for pure and mixed systems. The conductivity cell was calibrated before the measurements with standard solutions of potassium chloride (KCl) across a 0.01 M to 0.1 M concentration range. A concentrated gemini surfactant stock solution (10−20 times of the CMC) was progressively added using a micropipette to the IL 4-PyC8 solution. After thorough mixing and temperature equilibration at 300 K, the observed conductance was measured at each addition. The breakpoint of the plot of specific conductivity (κ) versus gemini surfactant concentration corresponded to the CMC for the pure gemini surfactant and mixed systems.

Surface Tension Measurements
The surface tension at different concentrations of gemini surfactants in the IL 4-PyC8 media was measured by using a KYOWA automatic surface tensiometer (DY-300) equipped with a platinum ring. The platinum ring was dried using a burner after every measurement to remove the sublimate of the gemini surfactant and the IL 4-PyC8. The surface tensiometer was calibrated by taking the surface tension of Millipore water (72 mN‧m −1 at 300 K). A 10 mL sample of 4-PyC8 solution was taken in a double-wall jacketed container, and the concentrated gemini surfactant of known concentration (below and above the CMC 10-15 times) was added progressively. The maximum force with which the platinum ring was pulled out from the sample was noted to be the surface tension γ (mN‧m −1 ) of the particular solution.

Fluorescence Measurements
All fluorescence spectra were measured using a Cary Eclipse Fluorescence (Agilent Technology) spectrophotometer. A strong hydrophobic fluorescent probe 1-pyrene carboxaldehyde (PyCHO) of 0.12 mM concentration was used in an aqueous micellar solution. The excitation wavelength was 334 nm and all fluorescence emission spectra were measured in the range of 340-450 nm. The excitation and emission slit widths were kept at 2.5 nm. Cetylpyridinium chloride (CPC) (0.12 mM) was used as the quencher to calculate the aggregation number.

FTIR Spectroscopy
The FTIR spectra of gemini surfactants and mixed systems were measured on a Bruker-ECO-ATR (attenuated total reflection) Model-i55 FTIR Spectrophotometer. In the mixed systems, the solid films were prepared via the evaporation of a 1:1 ratio waterethanol solution by stirring the mixture for several minutes and allowing it to dry overnight at room temperature. All the spectra were obtained over the spectral range of 4000-400 cm −1 at 298 K. Scheme 2. Synthesis of IL 4-((hydroxyimino)methyl)-1-(2-(octylamino)-2-oxoethyl) (4-PyC8).

Conductivity
A digital conductivity meter (Systronics, Type 304) equipped with a conductivity cell of cell constant 1.01 cm −1 was used to measure the conductance for pure and mixed systems. The conductivity cell was calibrated before the measurements with standard solutions of potassium chloride (KCl) across a 0.01 M to 0.1 M concentration range. A concentrated gemini surfactant stock solution (10−20 times of the CMC) was progressively added using a micropipette to the IL 4-PyC8 solution. After thorough mixing and temperature equilibration at 300 K, the observed conductance was measured at each addition. The breakpoint of the plot of specific conductivity (κ) versus gemini surfactant concentration corresponded to the CMC for the pure gemini surfactant and mixed systems.

Surface Tension Measurements
The surface tension at different concentrations of gemini surfactants in the IL 4-PyC8 media was measured by using a KYOWA automatic surface tensiometer (DY-300) equipped with a platinum ring. The platinum ring was dried using a burner after every measurement to remove the sublimate of the gemini surfactant and the IL 4-PyC8. The surface tensiometer was calibrated by taking the surface tension of Millipore water (72 mN·m −1 at 300 K). A 10 mL sample of 4-PyC8 solution was taken in a double-wall jacketed container, and the concentrated gemini surfactant of known concentration (below and above the CMC 10-15 times) was added progressively. The maximum force with which the platinum ring was pulled out from the sample was noted to be the surface tension γ (mN·m −1 ) of the particular solution.

Fluorescence Measurements
All fluorescence spectra were measured using a Cary Eclipse Fluorescence (Agilent Technology) spectrophotometer. A strong hydrophobic fluorescent probe 1-pyrene carboxaldehyde (PyCHO) of 0.12 mM concentration was used in an aqueous micellar solution. The excitation wavelength was 334 nm and all fluorescence emission spectra were measured in the range of 340-450 nm. The excitation and emission slit widths were kept at 2.5 nm. Cetylpyridinium chloride (CPC) (0.12 mM) was used as the quencher to calculate the aggregation number.

FTIR Spectroscopy
The FTIR spectra of gemini surfactants and mixed systems were measured on a Bruker-ECO-ATR (attenuated total reflection) Model-i55 FTIR Spectrophotometer. In the mixed systems, the solid films were prepared via the evaporation of a 1:1 ratio water-ethanol solution by stirring the mixture for several minutes and allowing it to dry overnight at room temperature. All the spectra were obtained over the spectral range of 4000-400 cm −1 at 298 K.

1 H NMR Measurements
1 H NMR measurements were recorded using a Bruker Avance III NMR spectrometer (400 MHz) in D 2 O.

Dynamic Light Scattering Study
The hydrodynamic diameter, size distribution and zeta potential of the aggregates were obtained using a Zetasizer Nano light scattering instrument (Malvern Instruments) with a He-Ne laser (633 nm, 10 mW). The scattering intensity was measured at θ = 173 • . The data obtained were processed using the Malvern DTS Software 7.11 package.

Determination of the Critical Micelle Concentration
The CMC values were determined for both the gemini surfactants 16-10-16 and 16-12-16 in the presence of 0.2, 0.5, 0.7 and 1 wt% of 4-PyC8 using the conductivity and surface tension.

Conductivity
We investigated the aggregation properties of gemini surfactants in water in the presence and absence of the IL 4-PyC8. The breakpoint of the plot between the specific conductivity (κ) and gemini surfactant concentration gave the CMC of the gemini surfactant and mixed systems, see Figure 1. The aggregation of the surfactant formed at the breakpoint. The counter ion dissociation (α) of gemini surfactants for the pure and mixed system (gemini surfactants/IL 4-PyC8) were also evaluated ( Table 1) from the ratio of the postmicellar and pre-micellar concentration range slopes obtained from the plots of specific conductance of the surfactant solution at different concentrations [41]:

Dynamic Light Scattering Study
The hydrodynamic diameter, size distribution and zeta potential of the aggregates were obtained using a Zetasizer Nano light scattering instrument (Malvern Instruments) with a He-Ne laser (633 nm, 10 mW). The scattering intensity was measured at θ = 173°. The data obtained were processed using the Malvern DTS Software 7.11 package.

Determination of the Critical Micelle Concentration
The CMC values were determined for both the gemini surfactants 16-10-16 and 16-12-16 in the presence of 0.2, 0.5, 0.7 and 1 wt% of 4-PyC8 using the conductivity and surface tension.

Conductivity
We investigated the aggregation properties of gemini surfactants in water in the presence and absence of the IL 4-PyC8. The breakpoint of the plot between the specific conductivity (κ) and gemini surfactant concentration gave the CMC of the gemini surfactant and mixed systems, see Figure 1. The aggregation of the surfactant formed at the breakpoint. The counter ion dissociation (α) of gemini surfactants for the pure and mixed system (gemini surfactants/IL 4-PyC8) were also evaluated ( Table 1) from the ratio of the post-micellar and pre-micellar concentration range slopes obtained from the plots of specific conductance of the surfactant solution at different concentrations [41]: It was observed that on increasing the mass fraction of the IL 4-PyC8, the α values gradually decreased. The CMC and α values are listed in Table 1. It was observed that an increase in the concentration of the IL 4-PyC8 led to a sharp decrease in the CMC value for both gemini surfactants. Increasing the mass fraction of the IL 4-PyC8 lowered the electrostatic repulsion between the charged head groups of the gemini surfactant and reduced the CMC, which favored micellization [42,43].  It was observed that on increasing the mass fraction of the IL 4-PyC8, the α values gradually decreased. The CMC and α values are listed in Table 1. It was observed that an increase in the concentration of the IL 4-PyC8 led to a sharp decrease in the CMC value for both gemini surfactants. Increasing the mass fraction of the IL 4-PyC8 lowered the electrostatic repulsion between the charged head groups of the gemini surfactant and reduced the CMC, which favored micellization [42,43].

Surface Tension
The surface tension versus the logarithm of the gemini surfactants concentration (M) plots are shown in Figure 2. The decrease in surface tension due to the adsorption of the gemini surfactant in the air/water interface is shown in Figure 2. At a constant gemini surfactant concentration, the surface tension gradually decreased when increasing the IL 4-PyC8 content. The surface tension decreased more rapidly, which may have been due to the longer alkyl chain IL increasing the hydrophobicity in the system [44,45]. Adding the IL 4-PyC8 caused the compression of the diffuse electric double layer, which decreased the electrostatic repulsions between the head groups of the gemini surfactants and reduced the CMC [46]. The CMC values obtained for the two gemini surfactants and mixed systems are listed in Table 1.

Surface Tension
The surface tension versus the logarithm of the gemini surfactants concentration (M) plots are shown in Figure 2. The decrease in surface tension due to the adsorption of the gemini surfactant in the air/water interface is shown in Figure 2. At a constant gemini surfactant concentration, the surface tension gradually decreased when increasing the IL 4-PyC8 content. The surface tension decreased more rapidly, which may have been due to the longer alkyl chain IL increasing the hydrophobicity in the system [44,45]. Adding the IL 4-PyC8 caused the compression of the diffuse electric double layer, which decreased the electrostatic repulsions between the head groups of the gemini surfactants and reduced the CMC [46]. The CMC values obtained for the two gemini surfactants and mixed systems are listed in Table 1.

Effect of Oxime-Functionalized Ionic Liquid on the Interfacial Properties
The maximum surface excess (Γ max ), minimum surface area per molecule (A min ), surface tension at CMC (γ cmc ) and surface pressure at the CMC (Π cmc = γ 0 − γ cmc ) of the gemini surfactants in the presence of 4-PyC8 were determined using tensiometry. All these data are summarized in Table 2. The values of the maximum surface excess (Γ max ) [33,47] were calculated using the Gibbs adsorption isotherm (Equation (2)): where dγ d log C denotes the surface activity at a temperature (in Kelvin) and R is the universal gas constant (8.314 J·K −1 ·mol −1 ). T is the absolute temperature in Kelvin, C is the concentration of the gemini surfactant and γ is the surface tension at a given concentration of surfactant. Since the gemini surfactant is made up of a divalent surfactant ion and two univalent counter-ions, the constant 'n' (pre-factor) value is taken as 3. The values of Γ max decreased with increasing the mass fraction of 4-PyC8 ( Table 2). The values of the minimum area per molecule (A min ) [33,48] of the gemini surfactant at the air-liquid interface were obtained using Equation (3): where N A is the Avogadro's number (6.022 × 10 23 mol −1 ) and Γ max is the maximum surface excess concentration (mol m −2 ) of adsorbed surfactant molecules at the interface. The A min values of the mixed systems composed of one of the two gemini surfactants and the IL 4-PyC8 are presented in Table 2. The results indicated that the values of Γ max and A min varied with the addition of the ionic liquid, revealing a lower Γ max (larger A min ) with a higher content of IL. The values of Γ max decreased and A min increased due to the reduction in forces between the two head groups of the gemini surfactants in the presence of the IL 4-PyC8 when the molecules were less compactly packed at the air/water interface.
The surface pressure at the CMC (π cmc ) [49] is a measure of the surface tension reduction at the CMC and is calculated using Equation (4): where γ 0 and γ cmc correspond to pure water and the mixed system at the CMC at 300 K.
The π cmc values depend on the interfacial area occupied by the gemini surfactants with their specific position and the structure at the interface. The reduction of the surface tension

Effect of Oxime-Functionalized Ionic Liquid on Thermodynamic Parameters
Oxime-functionalized IL 4-PyC8 modified the physicochemical and thermodynamic properties of both gemini surfactants. The intermolecular forces, such as Van der Waals forces, dipole-dipole interaction and hydrogen bonding, were involved in the interaction of the IL 4-PyC8 with the gemini surfactants. The standard Gibbs free energy of micellization (∆G • m ) was calculated for the analysis of the IL 4-PyC8 on the micellization process [50] using the following Equation (5): where α is the degree of counterion dissociation, R is the ideal gas constant, T is the temperature in Kelvin and X CMC is the CMC in the mole fraction unit. The value of ∆G • m became more negative as the IL 4-PyC8 concentration increased, which was due to stronger hydrophobic interactions. The calculated value of ∆G • m is listed in Table 3. The negative value of ∆G • m led to the spontaneous process. The standard Gibbs free energy of adsorption (∆G • ads ) at the air/water interface [51] was calculated by using Equation (6): where ∆G • m is the Gibbs free energy of micellization, Γ max is the maximum surface excess concentration and π CMC is the surface pressure at the CMC. Here, the addition of the IL 4-PyC8 led to an increase in ∆G • ads , which indicated the stronger hydrophobic interaction due to the repulsion forces in the water/hydrophobic interface. The ∆G • ads supported the micellization behavior between the IL 4-PyC8 and the gemini surfactants. The ∆G • ads values also favored the spontaneous micellization process. All these values are listed in Table 3. The free energy of the given air/water interface ∆G (s) min [52] was found using Equation (7): Chemosensors 2022, 10, 46 9 of 21 ∆G (s) min indicates the free energy change of the solution components from the bulk phase to the surface phase of the solution. Lower ∆G (s) min values were reported to indicate [34] a more thermodynamically stable micellar surface. All thermodynamic parameters are given in Table 3. The Gibbs free energy of the micellization per alkyl tail may be expressed as (see Equation (8)) [53]: The gemini surfactant tail transfers Gibbs free energy from the solvent mixture to the hydrophobic core of the micelle. The tail of the gemini surfactant detached due to the solvophobic effects. The effect of the IL 4-PyC8 on the micellization process was also evaluated through the Gibbs energy of transfer (∆G • trans ), given by Equation (9) [52]: As compared with the pure solvent, the addition of the IL 4-PyC8 made the thermodynamics more favorable for the gemini surfactant molecule and the hydrophobic tail part to move from the bulky phase into the micellar phase. As a result, ∆G • trans decreased with the decrease in the CMC values of the gemini surfactants.

Fluorescence Measurements
We used 1-pyrene carboxyaldehyde (PyCHO) as a fluorescence probe to obtain the CMC of the gemini surfactants in the presence and absence of the IL 4-PyC8. The fluorescence emission spectra exhibited a characteristic band near 450 nm. PyCHO fluorescence has been used to measure various important micellar parameters [54][55][56]. Fluorescence spectra were obtained from solutions of varying gemini surfactants in the presence of different mass fractions of the IL 4-PyC8. The CMC was determined by plotting the intensity against the gemini surfactant concentration (Figure 3). It is noteworthy to mention that the CMC of the gemini surfactants decreased with the increasing mass fraction of the IL 4-PyC8 (see Table 1). The CMC values determined using this method were slightly larger than those determined via the conductance and surface tension (see Table 1), which may have been due to the presence of the PyCHO in the micro-organized system [45,46]. The free energy of the given air/water interface ΔG (s) min [52] was found using Equation (7): ΔG (s) min indicates the free energy change of the solution components from the bulk phase to the surface phase of the solution. Lower ΔG (s) min values were reported to indicate [34] a more thermodynamically stable micellar surface. All thermodynamic parameters are given in Table 3. The Gibbs free energy of the micellization per alkyl tail may be expressed as (see Equation (8)) [53]: The gemini surfactant tail transfers Gibbs free energy from the solvent mixture to the hydrophobic core of the micelle. The tail of the gemini surfactant detached due to the solvophobic effects. The effect of the IL 4-PyC8 on the micellization process was also evaluated through the Gibbs energy of transfer (ΔG°trans), given by Equation (9) As compared with the pure solvent, the addition of the IL 4-PyC8 made the thermodynamics more favorable for the gemini surfactant molecule and the hydrophobic tail part to move from the bulky phase into the micellar phase. As a result, ΔG°trans decreased with the decrease in the CMC values of the gemini surfactants.

Fluorescence Measurements
We used 1-pyrene carboxyaldehyde (PyCHO) as a fluorescence probe to obtain the CMC of the gemini surfactants in the presence and absence of the IL 4-PyC8. The fluorescence emission spectra exhibited a characteristic band near 450 nm. PyCHO fluorescence has been used to measure various important micellar parameters [54][55][56]. Fluorescence spectra were obtained from solutions of varying gemini surfactants in the presence of different mass fractions of the IL 4-PyC8. The CMC was determined by plotting the intensity against the gemini surfactant concentration (Figure 3). It is noteworthy to mention that the CMC of the gemini surfactants decreased with the increasing mass fraction of the IL 4-PyC8 (see Table 1). The CMC values determined using this method were slightly larger than those determined via the conductance and surface tension (see Table 1), which may have been due to the presence of the PyCHO in the micro-organized system [45,46].

Aggregation Numbers
The aggregation behavior of the gemini surfactants and their interaction with the IL 4-PyC8 have also been studied via static fluorescence quenching measurements, which is a fundamental parameter of micellar properties, by using the following Equation (10):  [57]. These factors held the micellization environment of the gemini surfactants in the presence of the IL 4-PyC8 [58]. We may suggest that between the two gemini surfactants, 16-12-16 was slightly more hydrophobic than 16-10-16, which resulted in the IL 4-PyC8 being able to transfer more successfully into the micelles of the gemini surfactant 16-10-16 [34].

Aggregation Numbers
The aggregation behavior of the gemini surfactants and their interaction with the IL 4-PyC8 have also been studied via static fluorescence quenching measurements, which is a fundamental parameter of micellar properties, by using the following Equation (10):  [57]. These factors held the micellization environment of the gemini surfactants in the presence of the IL 4-PyC8 [58]. We may suggest that between the two gemini surfactants, 16-12-16 was slightly more hydrophobic than 16-10-16, which resulted in the IL 4-PyC8 being able to transfer more successfully into the micelles of the gemini surfactant 16-10-16 [34]. The nature of the hydrophobicity of the gemini surfactants could be calculated using the Stern-Volmer quenching constant (KSV) (Equation (11)). The KSV values are given in Table 4.  The nature of the hydrophobicity of the gemini surfactants could be calculated using the Stern-Volmer quenching constant (K SV ) (Equation (11)). The K SV values are given in Table 4. The Stern-Volmer quenching constant (K SV ) can be assessed using the plots of ln(I 0 /I Q ) versus [Q]. A difference in the K SV values was observed, which can be explained using the base hydrophobicity of the micellar environment [55]. The higher the solubility of the probe and a quencher, the higher the K SV value. The aggregation numbers and K SV values are collected in Table 4.
The CMC values obtained using fluorometric techniques and collected in Table 4 were consistent with those obtained from the tensiometry and conductivity data (see Table 1).

FTIR Spectroscopy
The interaction between the oxime-functionalized IL 4-PyC8 and the gemini surfactants was studied using FTIR spectroscopy. The FTIR spectra of pure 16-s-16 and the IL 4-PyC8 are shown in Figures 5 and 6. In both spectra of 16-10-16 and 16-12-16, as well as for the IL 4-PyC8, there was no sharp peak at~3450-3700 cm −1 , which shows that no water was present in the sample. The FTIR spectrum of 16-10-16 ( Figure 5) showed a strong peak at the wavenumber 1467.97 cm −1 , which was due to C-N stretching. The different stretching bands observed were the symmetric and asymmetric stretching of the CH 2 vibration of alkyl chains at 2917.27 cm −1 and 2850.08 cm −1 , the symmetric and asymmetric stretching of the C-H scissoring vibration of the CH 3 -N + moiety at 1467.97 cm −1 , the C-N + stretching bands' rocking mode of the methylene chain at 889.09 cm −1 and the rocking mode of the methylene chain at 721.45 cm −1 . After the addition of the IL 4-PyC8, the stretching frequencies were changed to 2917.72 cm −1 , 2849.32 cm −1 , 1466.07 cm −1 , 889.75 cm −1 and 720.80 cm −1 , respectively. More importantly, the C=O stretching frequency for the IL 4-PyC8 at 1666 cm −1 was shifted to 1676 cm −1 after mixing with the gemini surfactant 16-10-16. In the FTIR spectrum of the 16-12-16 gemini surfactant (shown in Figure 6), different frequencies were observed, see Table 5: symmetric and asymmetric stretching of the CH 2 vibration of alkyl chains at 2917.32 cm −1 and 2848.35 cm −1 , symmetric and asymmetric stretching of the C-H scissoring vibration of the CH 3 -N + moiety at 1468.53 cm −1 , the C-N + stretching bands' rocking mode of the methylene chain at 889.07 cm −1 and the rocking mode of the methylene chain at 721.96 cm −1 . For the mixture of the IL 4-PyC8 and gemini surfactants during the process of micellization, the stretching frequency values were changed to 2921.55 cm −1 , 2852.26 cm −1 , 1464.31 cm −1 , 889.60 cm −1 and 720.98 cm −1 , respectively. The shift of the wavenumber in the mixture of the gemini surfactant 16-12-16 and the IL 4-PyC8 represented some changes that occurred within the system. These variations in these peaks may have been due to +N-(CH 3 ) or NH 2 stretching. The observed difference in the wavenumber indicated that the additive IL 4-PyC8 interacted with the gemini surfactants and it may have been due to some of the structural changes that occurred within the IL 4-PyC8:gemini surfactant mixed system. It is noteworthy that the C=O stretching frequency for 4-PyC8 at 1666 cm −1 was shifted to 1681 cm −1 after mixing with the gemini surfactant 16-12-16, indicating an IL:gemini surfactant mixed system. The changes in the IR spectra could be considered as non-specific interactions (ion-ion dipole and induced dipole interactions) between the gemini surfactants and IL that took part in the complexation, similar to what is reported in the literature [56] and in accordance with the 1 H NMR data discussed above.
Chemosensors 2022, 10, x FOR PEER REVIEW 12 of 21 in these peaks may have been due to +N-(CH3) or NH2 stretching. The observed difference in the wavenumber indicated that the additive IL 4-PyC8 interacted with the gemini surfactants and it may have been due to some of the structural changes that occurred within the IL 4-PyC8:gemini surfactant mixed system. It is noteworthy that the C=O stretching frequency for 4-PyC8 at 1666 cm −1 was shifted to 1681 cm −1 after mixing with the gemini surfactant 16-12-16, indicating an IL:gemini surfactant mixed system. The changes in the IR spectra could be considered as non-specific interactions (ion-ion dipole and induced dipole interactions) between the gemini surfactants and IL that took part in the complexation, similar to what is reported in the literature [56] and in accordance with the 1 H NMR data discussed above.    1 H NMR techniques are well-known to give information about the aggregate microenvironment and this can be used to probe the surfactant self-assembly of different structures [61]. Although NMR spectroscopy is a very useful technique, it can hardly be   [61]. Although NMR spectroscopy is a very useful technique, it can hardly be expected to recognize the changes in the chemical shifts of the side-chain protons. The hydrophobic tails form the micellar "core", whereas the detectable changes in the chemi-cal shifts occur mainly in the region close to the interface water/aggregate (e.g., micelle, vesicle). Aromatic protons can be very sensitive to the changes in the microenvironment of the surfactant aggregate [62]. Deprotonation of the oxime moiety due to changes in the micellar micropolarity or tight ion pair formation may also be detected using the changes in chemical shifts of the adjacent groups [61]. The 1 H NMR spectra of the gemini surfactants with the IL 4-PyC8 in D 2 O are presented in Figure 7 (for 16-10-16) and Figure 8 (for 16-12-16). We note that no specific interaction confirmed by the changes in the chemical shifts could be reported based on these data; the surfactant aggregates were supposed to form under these concentration conditions, as gemini surfactants aggregates ([D] 0 >> CMC) with IL molecules were solubilized by the micelles.
Chemosensors 2022, 10, x FOR PEER REVIEW 14 of 21 expected to recognize the changes in the chemical shifts of the side-chain protons. The hydrophobic tails form the micellar "core", whereas the detectable changes in the chemical shifts occur mainly in the region close to the interface water/aggregate (e.g., micelle, vesicle). Aromatic protons can be very sensitive to the changes in the microenvironment of the surfactant aggregate [62]. Deprotonation of the oхime moiety due to changes in the micellar micropolarity or tight ion pair formation may also be detected using the changes in chemical shifts of the adjacent groups [61]. The 1 H NMR spectra of the gemini surfactants with the IL 4-PyC8 in D2O are presented in Figure 7 (for 16-10-16) and Figure 8 (for 16-12-16). We note that no specific interaction confirmed by the changes in the chemical shifts could be reported based on these data; the surfactant aggregates were supposed to form under these concentration conditions, as gemini surfactants aggregates ([D]0 >> CMC) with IL molecules were solubilized by the micelles.

Dynamic Light Scattering and Zeta Potential Study of Mixed Gemini:IL System with PMZ
Taking into account the interactions between the oxime-derived IL and another phe-

Dynamic Light Scattering and Zeta Potential Study of Mixed Gemini:IL System with PMZ
Taking into account the interactions between the oxime-derived IL and another phenothiazine drug called promazine that was reported recently [42], an evaluation of the structural changes of the aggregates of oxime IL/gemini surfactants with the addition of promethazine was carried out. Dynamic light scattering (DLS) studies were performed to shed light on the sizes of the self-assembly aggregates of the various concentrations of the IL 4-PyC8 that were added to the gemini surfactant solution in the presence of the drug PMZ. The aggregate size may be dependent on the solubilizate structure, as well as the nature and concentration of the surfactant, and using a low gemini concentration close to the CMC is not suitable since aggregates of different sizes and hardly defined structures may form [63]. The concentration of the gemini surfactant 16-10-16 was taken significantly above the CMC value (1 mM). The DLS results provided us with information on the size of the gemini surfactant aggregates in the presence and absence of the drug and the IL 4-PyC8. It was observed that the hydrodynamic diameter of 1 mM aqueous gemini surfactant (ca. 35 × CMC in the case of 16-10-16) solution without any other additives was observed to be about 124 nm (see Figure 9a), which was much larger than that of a regular spherical or ellipsoidal micelle [64]. Indeed, gemini surfactants are known to undergo a fast sphere-to-rod transition above the CMC [65], form unexpectedly large aggregates in the presence of polyaromatics [63,66,67] and to assemble into wormlike micelles [67], as well as other complex aggregates [68], at concentrations substantially above the CMC. As it was shown by Pisarcik et al. [69], the extension of the spacer length in the diamide gemini surfactant molecule to s > 6 results in a sharp increase in the N agg with a concentration above the value of 2 × CMC. Furthermore, surfactants with the spacer length of n = 8 were reported to form aggregates of >100 nm at the concentration of 6 × CMC. Since the aggregation numbers were not large enough to expect vesicle formation, Pisarcik et al. [69] suggested the hydrodynamic size reflected the intermicellar aggregates. We supposed the 16-10-16 surfactants reported in the present work may demonstrate similar behavior at the studied concentration. An addition of PMZ (0.01 mM) to the gemini micellar solution resulted in a significant increase in the aggregate size from 124 nm to 270 nm. It is noteworthy to mention that the addition of 1 wt% of the oxime-functionalized IL 4-PyC8 to the above gemini micellar solutions with the drug added resulted in a drastic structural transition from such larger aggregates of 270 nm to small-sized micelles with sizes of 1 to 2 nm (see Figure 9f). The size distribution at 1-2 nm might correspond to the micelles but is smaller than the real size of the micelles in this technique, which may be ascribed to the high charge density of the gemini surfactant micelles that affects the DLS measurement [70]. Initially, the addition of the drug produced a significant increase in the micellar size due to the partition of the drug within the (inter)micellar aggregate interior region, but the addition of the IL 4-PyC8 to this system caused drastic changes in the micellar size of the gemini:IL:PMZ system. These changes in the self-assembly structural transition may indicate stronger IL:PMZ, gemini:IL and gemini:PMZ interactions.
The values of the zeta potential measurement (ξ (mV)) in these systems depend on the difference in potential between the dispersion medium and on the stationary liquid layer that is attached to the dispersed aggregates [71]. Initially, the value of ξ insignificantly increased with the addition of drug (24.8 mV) to the gemini micellar solution (22.6 mV), reflecting the positive charges on the overall surface of the carrier. Upon the addition of IL4-PyC8 to the gemini:PMZ system, the value of ξ slightly decreased (Table 6). This may have reflected the strong electrostatic interactions that took place between the oppositely charged ions present within the micellar system [72]. As a result, a different self-assembly shape appeared, which caused the lowering of the values of ξ [63,73]. Further, adding higher concentrations of the IL 4-PyC8 (1.0 wt%) into the mixture of the gemini:PMZ solution resulted in an increase in the surface charge density. The overall strong interactions (16-s-16:IL:PMZ) led to the significant structural changes of gemini aggregates in the presence of 1 wt% of the oxime-functionalized IL 4-PyC8 to the gemini surfactant solutions with the drug added. There was a correlation between the surface potential and the aggregates' sizes and morphologies, as the aggregates were formed with varying morphologies [74] in the presence of the drug and the IL 4-PyC8. above gemini micellar solutions with the drug added resulted in a drastic structural transition from such larger aggregates of 270 nm to small-sized micelles with sizes of 1 to 2 nm (see Figure 9f). The size distribution at 1-2 nm might correspond to the micelles but is smaller than the real size of the micelles in this technique, which may be ascribed to the high charge density of the gemini surfactant micelles that affects the DLS measurement [70]. Initially, the addition of the drug produced a significant increase in the micellar size due to the partition of the drug within the (inter)micellar aggregate interior region, but the addition of the IL 4-PyC8 to this system caused drastic changes in the micellar size of the gemini:IL:PMZ system. These changes in the self-assembly structural transition may indicate stronger IL:PMZ, gemini:IL and gemini:PMZ interactions.  The values of the zeta potential measurement (ξ (mV)) in these systems depend on the difference in potential between the dispersion medium and on the stationary liquid layer that is attached to the dispersed aggregates [71]. Initially, the value of ξ insignificantly increased with the addition of drug (24.8 mV) to the gemini micellar solution (22.6 mV), reflecting the positive charges on the overall surface of the carrier. Upon the addition of IL4-PyC8 to the gemini:PMZ system, the value of ξ slightly decreased (Table 6). This may have reflected the strong electrostatic interactions that took place between the oppositely charged ions present within the micellar system [72]. As a result, a different selfassembly shape appeared, which caused the lowering of the values of ξ [63,73]. Further, adding higher concentrations of the IL 4-PyC8 (1.0 wt%) into the mixture of the

Conclusions
The design and application of sustainable surfactants in the remediation of contaminated areas were considered regarding promethazine, a chiral pharmaceutical pollutant. The micellization behavior and physicochemical properties of the gemini surfactants 16-10-16 and 16-12-16 in the presence of the functionalized IL 4-PyC8 were investigated using surface tension, conductivity, fluorescence, FTIR, 1 H NMR and DLS techniques. The surface tension and conductometry results revealed a decrease in the CMC of both the gemini surfactants in the presence of the IL 4-PyC8, thus favoring the micelle formation process. The negative values of ∆G 0 m and ∆G 0 ads indicated that the micellization process was spontaneous. The aggregation number (N agg ) and Stern-Volmer constant (K SV ) of the gemini surfactants decreased with the increasing mass fraction of the IL 4-PyC8. The cmc and N agg results may be ascribed to the presence of counterions near the polar heads of the gemini surfactant molecules that tended to decrease the electrostatic repulsion forces between the gemini surfactant head groups, leading to the compact aggregation of surfactant monomers. The aggregate sizes obtained from the DLS measurements demonstrated that the addition of 1 wt% of the oxime-functionalized IL 4-PyC8 to the gemini surfactant solutions in the presence of promethazine may have resulted in a structural transition from very large aggregates (270 nm) to small compact micelles following strong gemini:IL:PMZ interactions. The structural transitions in the presence of promethazine reported in the present work may be used for designing systems responsive to changes in the size and shape of the aggregates as an analytical signal for the selective binding of biologically important molecules and potential chiral pollutants. Informed Consent Statement: Not applicable.
Data Availability Statement: All the data gathered for this study are available in the article.