Effects of an Interdisciplinary Course on Pre-Service Primary Teachers’ Content Knowledge and Academic Self-Concepts in Science and Technology–A Quantitative Longitudinal Study

Primary school teachers need adequate professional knowledge and motivational orientations to qualify scientifically and technologically literate students. Previous studies have focused on the impact of coursework on (pre-service) primary teachers’ content knowledge, rather than on the development of academic self-concepts. In addition, the influence of the course format and the major field of study has not been investigated much to this date. Thus, this study examines the effects of an interdisciplinary course on pre-service primary teachers’ content knowledge and academic self-concepts in science and technology using a quasi-experimental, quantitative, pre-post-follow-up design (n = 202). Whilst no significant changes in knowledge were revealed for the baseline group not participating in the course, significant short-term and long-term cognitive gains were found for the experimental group. Biology-, chemistry-, physics- and technology-related self-concepts increased significantly when participating in the course. The results also indicate that the course format and major field of study can have an impact on the development of pre-service teachers’ professional knowledge. Regarding the development of academic self-concepts in the experimental group, it can be assumed that both the weekly and block course format are beneficial for heterogeneous learner groups consisting of pre-service teachers with different major fields of study.


Introduction
Worldwide, the aim of science and technology education in primary school is to develop pupils' scientific [1] and technological literacy [2]. Children should acquire competencies enabling them to participate and act responsibly in this science-and technologyoriented, multi-perspective world. Every day, they encounter numerous phenomena such as rainbows, the "disappearance" of water from its puddle or the floating of a giant ship in a river. Primary science and technology lessons are intended to help them better understand these phenomena. However, it is not just about gaining knowledge and procedural skills, being familiar with some of the fundamental concepts and fostering conceptual change to overcome non-scientific concepts. The goal is also to contribute to positive motivational orientations, thus reinforcing self-confidence and interest in science and technology [2][3][4][5][6][7].
In addition to pedagogical content knowledge (PCK) and pedagogical knowledge (PK), primary school teachers need adequate content knowledge (CK) in the domains of biology, chemistry, physics and technology to plan and deliver instruction that meets the above goals [8][9][10][11]. CK is seen as a basis for the development of PCK and influences the actions of teachers (for details, see Section 1.1). If a primary teacher wants children to explore natural phenomena, he/she needs CK in all related disciplines to design the lesson 1.
Teacher generalist training and teaching-out-of-field: In many countries, primary teachers are educated as generalists who have to teach multiple subjects [29,40,41]. However, training in three to four or more subjects also implies fewer contact hours per subject [42][43][44][45]. This is problematic for the goal of increasing CK and ASCs-positively correlating with the corresponding CK domain (see Section 1.2)-, as it has been shown that the number of contact hours with the subject at the university correlates positively with the level of CK [46]. Primary teachers who took a science major during their studies have a higher science CK than primary teachers without this major [22,45]. In some countries, such as China [7] or Germany [47], science and technology are sometimes not covered at all during studies [43][44][45]. Nevertheless, primary school teachers then have to teach topics from these disciplines in their role as classroom teachers [48].

2.
Subject-integrated teaching-broad study content: With the aim of exploring the multi-perspective world (see above), in many countries, including Japan, France, Austria, The Netherlands, Slovenia, China and Germany, science and technology content is taught within an integrated subject in primary school [7,45,49,50]. By the term "integrative" we mean that the subject includes not only biology, chemistry, physics and (sometimes) technology, but also disciplines such as history, social sciences, geography [7,45,[49][50][51]. Since this subject is called differently in each country [45], the term 1.2. Teachers' Academic Self-Concept: Definition, Structure, Operationalization, Relevance and Influencing Factors Hierarchically structured and multifaceted, self-concept is a person's perception of her-or himself [108]. It arises from the personal set of knowledge, skills and beliefs and is formed through experiences with and influences of the environment [108][109][110]. The general self-concept can be differentiated into a non-academic and academic self-concept (ASC). The latter includes at least two facets-verbal and mathematic ASC-which can be divided into different subject areas [111,112]. The chemistry-and physics-related ASC are assigned to the math ASC, while the biology-related ASC can be allocated to both the verbal and the math ASC [113,114]. According to Marsh's classification [113], it is to be assumed that the technology-related ASC can at least be subordinated to the mathematical ASC due to its proximity to the subjects of physics and mathematics [115].
Little is known about the structure of teachers' ASC, defined as self-evaluation of their professional knowledge [116,117]. The findings of Paulick et al. [116] reveal that teachers' ASC can be subdivided into CK-, PCK-and PK-related components. Regarding science-and technology-related CK ASCs of pre-or in-service primary teachers, previous studies have either focused on one domain-mostly physics [20,118,119]-or their ASC has been examined without checking whether mental discrimination is made between the domains [32,120]. However, primary school teachers may not have science domainspecific ASCs at all due to the integrative character of General Studies [3]. Gabriel-Busse Educ. Sci. 2021, 11, 744 5 of 38 et al. [121] showed that German primary school children discriminate between science-, technology-, history-, geography-and politics-related ASCs, but not between their selfevaluated abilities in biology, chemistry and physics.
ASC is considered a mediator between experiences of performance in a particular domain and (learning) behavior or motivation related to this domain [122]. A pre-service teacher who has had negative learning experiences with physics in school or the course of study and, as a result, develops a low physics-related ASC [18] is likely to exhibit low learning motivation and engagement in this area in the future [110,123,124]. Therefore, it is not surprising that teachers with a positive ASC show different teaching behaviors than teachers with a low ASC [21]. They are more likely to adopt new instructional practices [125], support the development of pupils' motivational orientations, such as their interests [21], and are more tolerant to stress [126]. The ASC also influences whether teachers avoid inquiry-based instructional settings and instead tend to provide expository instruction, which does little to stimulate deep learning [17,21,29]. Consequently, in terms of teaching effectiveness, high teacher ASCs can also lead to greater cognitive gains among pupils [127]. Teachers' ASCs also seem to be of relevance in developing other motivational orientations and mediating the interaction of interest and professional knowledge [117].
According to the reciprocal effects model [128], ASC and performance have a reciprocal, mutually reinforcing relationship. Thus, academic achievement impacts and predicts self-concept, and self-concept affects and predicts achievement. Positive correlations of professional knowledge and ASC among pre-and in-service teachers have already been shown for mathematics [129], biology and physics [116,117]. Paulick et al. [116] found positive relationships between CK ASC and CK test scores, PCK ASC and PCK test scores, and PK ASC and PK performance. They conclude that teachers' ASC is a predictor for their performance in the corresponding domain. However, the strength of correlations also suggests that additional factors are influencing their ASCs.
Due to the numerous favorable outcomes of ASCs, many educational interventions in school and colleges aim to develop them positively [32,118,130,131], although altering them is challenging as they are considered rather stable [15]. Little is known about possible factors that affect teachers' ASC during their university education. There is first evidence that external and internal reference norms [112] have an impact on pre-service teachers' ASCs [117,132]. Social comparisons (external reference), take place, for example, when a pre-service teacher compares his performance/skills in a university science course with the performance of fellow students in the same course [16,112,133]. Internal reference norms include temporal (comparison of current performance with past performance), dimensional (comparison of performance in one domain, e.g., biology, with performance in another domain, e.g., chemistry) and criterial (comparison of performance with target criteria, e.g., competence expectations for a teacher) comparisons [16,110,112,134]. In addition to a positive experience of competency, individual oral or written performance feedback and supportive faculty behaviors could also strengthen pre-service teachers' ASCs [16,110,135,136]. Furthermore, studies examining the experiment-related ASC [137,138] demonstrate that the major field of study may also be a variable that influences pre-service teachers' ASC.
For the positive development of ASCs with respect to more than one discipline and avoiding fragmented knowledge, interdisciplinary learning environments are considered beneficial [32,139,140]. This will be discussed in the next section.

Research on the Development of Pre-and In-Service Primary Teachers Science-and Technolgy-Related CK and ASCs through Interdisciplinary Interventions: Status Quo and Research Gaps
Interdisciplinary courses are seen as one way to address the challenges described in the introduction [32,43,140]. By the term "interdisciplinary" we mean that a course deals with topics and questions that require competencies from several disciplines, e.g., biology and technology [141].
Such interdisciplinary learning environments are expected to better connect learning content [139,140]. Aström showed for the school context that interdisciplinary learning Educ. Sci. 2021, 11, 744 6 of 38 has no disadvantages compared to disciplinary learning in terms of subject knowledge gain [142]. While several studies have revealed positive effects of science content or/and methods courses as well as teacher training on pre-and in-service primary teachers' CK [24,26,101,143], there are just a few reports of courses and their effectiveness that focus on more than one discipline of General Studies. Cross-sectional and longitudinal studies often investigate topic-or discipline-specific CK, e.g., CK on "states of matter and phase transitions" [96] or "combustion" [45]. However, this means that the ability to think across disciplines, as explicitly called for in the Minimum Standards for General Studies Teacher Education [13], cannot be tested at all or only to a very limited extent. For a crosssectional study, Niermann [144] developed a questionnaire to examine primary teachers' declarative CK on all five main domains of General Studies. Nevertheless, the questionnaire contains only one question per domain, and no interdisciplinary CK is required to answer the questions. One of the few longitudinal studies investigating change in pre-service primary teachers' interdisciplinary CK is the study by Kratz and colleagues [102]. Using a concept mapping technique, they demonstrate that interdisciplinary CK (biology and physics) increased significantly as a result of a university seminar linking biology and physics.
For the context of school, studies have shown that interdisciplinary science education leads to a significant increase in science-related ASCs, especially among girls, thereby eliminating the differences between girls and boys [141,145]. Given that the majority of primary school teachers are women [146], a discipline-integrating approach to their university education and training could be useful in positively influencing the often low chemistry-, physics-and technology-related ASCs.
However, there is a global lack of research on the effects of (interdisciplinary) courses on the development of science-and technology-related ASCs of pre-and in-service primary teachers [147]. Those longitudinal studies that do exist tend to focus on only one science domain, with an emphasis on physics-related ASC [118,119], and have yielded mixed results: Technology-related ASC [147] and physics-related ASC [119] of pre-service primary teachers did not change as a result of a single technology-related seminar or two physicsrelated experiment-based seminars, respectively. However, as in studies with pupils who participated in a half-day laboratory course [130,131], experiment-related ASC increased significantly among pre-service primary teachers in [119] as a result of participation in a one-semester course. Kleickmann and colleagues [118] found that five months of physicsrelated in-service primary teacher training with extensive, adaptive scaffolding led to higher physics-related ASCs. In a study involving a very small sample (n = 19; [120]), a one-semester course integrating biology and chemistry led to a significant increase in pre-service primary teachers' chemistry-related ASC. Biology-related ASC did not change significantly. Furthermore, Göhring [32] has shown that a program of study including several integrated science modules results in positive development especially of pre-service primary teachers' chemistry-and physics-related ASCs. As university studies progressed, the ASCs stabilized.

Research on the Development of Pre-and In-Service Teachers CK and ASCs through Different Course Formats: Status Quo and Research Gaps
In particular, pre-service primary teachers, who do not study General Studies or do not have a science and technology major, might be interested in block scheduled training, e.g., during the semester break, to enhance their professional competencies in the field of science and technology. Block course formats create curriculum flexibility and are suitable for certain student groups, such as students with parallel time-intensive commitments [148][149][150]. These "intensive" or "non-traditional" courses do not take place regularly once or twice a week, but in compact time blocks during the semester or lecturefree period. The total number of instructional hours is generally identical to that of "traditional", weekly courses [148,151].
Compared to the context of school, with very mixed results on the impact on academic achievement [152], there are few longitudinal studies on the effectiveness of different course formats in higher education, including teacher education [153]. Often, samples are relatively small [154,155] or only subjective impressions about experiences with the course formats are investigated [153,155,156]. Most of these studies show that higher education block courses lead to at least equivalent or even greater short-and long-term knowledge gains than weekly courses [154,[157][158][159][160]. Little research suggests that block teaching leads to poorer performance in higher education institutions [161,162]. One of the main arguments against the pedagogical value of block formats, frequently mentioned in the context of school, is the attention span of learners. During a block course, less learning content may be retained because attention diminishes [163,164]. Another counter-argument is that the learning content is better memorized and deepened in weekly courses since there is less input at once, regular processing and repetition [148,165,166].
In contrast to the effects on cognitive gain, there are almost no studies on the impact of the variable "course format" on pre-and in-service teachers' ASC. For the context of school education, Bateson [167] showed that there were significant differences in science achievement between grade-10 students attending different course formats, but no differences in affective domains. However, he examined science attitudes, not ASCs. [154,159] found that students who participated in a university block course had higher scores on perceived competence than those who participated in the weekly version. This may be an indication that the ASC develops more positively through block courses, as the experience of competency is considered an important factor influencing self-concept [108,135]. According to Burton and Nesbit [151], perception of the ability in a specific domain-in addition to experience with the course format and current subject load-also appears to influence whether students tend to take a block course in the appropriate domain.

Aim of the Study
As described above, primary teachers' CK and ASCs in biology, chemistry, physics and technology play an essential role in supporting pupil learning in General Studies classes [21,68,90,107]. However, pre-and in-service primary teachers' CK and ASCs in these disciplines tend to be low [17,18,20,[22][23][24][25][26][27][28]. Previous research (see Section 1.3) suggests that interdisciplinary courses have the potential to positively develop their CK and ASCs regarding several of these disciplines. The few existing research reports on effects of interdisciplinary interventions for pre-service primary teachers in the field of science and technology refer to entire programs of study [32] or examine the impact of a short-time intervention that links only two disciplines [102,120]. For the development of an adequate professional identity and genesis of discipline-interlinking CK [8,13], however, it would be appropriate to integrate all three science disciplines (biology, chemistry, physics) and technology, which is closely interwoven with the natural sciences [168], in a single course. With positive effects on the CK and ASCs, such interdisciplinary course offerings could be a solution for making more effective use of the limited time resources (see introduction) in the primary teacher education program [169].
Thus, this study investigates the impact of an interdisciplinary university course that integrates biology, chemistry, physics and technology (see Section 2.2) on pre-service primary teachers' CK and ASCs in science and technology. The first block of research questions is: RQ 1: The intervention's impact on CK and ASC in science and technology RQ 1.1: Does participation in the intervention lead to short-term and long-term gains in CK in science and technology compared to non-participation? RQ 1.2: Does participation in the intervention lead to a change in biology-, chemistry-, physics-and technology-related ASCs compared to non-participation?

RQ 2: Correlations between cognitive gain and changes in ASCs
Is there a positive correlation between cognitive gain and change in biology-, chemistry-, physics-and technology-related ASCs?
Referring to the findings of [116] that teachers' ASCs are indicators of their achievements in professional knowledge, the results on RQ 2 are used to assess the external validity of the cognitive test (see Section 2.4.1). Furthermore, studies on the effects of the course format (weekly versus block course format) are mostly situated in the context of schooling [152,170]. Few research reports relate to higher education teaching (see Section 1.4; [150]). The influence of this variable is particularly important with respect to further training of pre-and in-service primary teachers who do not have an appropriate university education in science and technology. Due to limited time during the semester or work life, they may be particularly interested in further training in a block format. Thus, the second block of research questions is: RQ 3: Impact of the course format RQ 3.1: Are there equivalent short-and long-term cognitive gains between preservice teachers attending a traditional/weekly and an intensive/block course format of the intervention? RQ 3.2: Does the course format impact the development of biology-, chemistry-, physics-and technology-related ASCs?
Finally, we examine whether both pre-service primary teachers with and without a major field of study in science and technology benefit from the intervention in terms of CK and ASCs. Given the differences in cognitive and affective baselines found in previous studies [22,45,137], it may be possible that one group is under-challenged and the other is over-challenged. RQ 4: Impact of the major field of study RQ 4.1: Does the major field of study affect short-and long-term cognitive gains within groups of weekly and block course participants? RQ 4.2: Does the major field of studies influence the development of biology-, chemistry-, physics-and technology-related ASCs within groups of weekly and block course participants?

Research Design
The cognitive and affective effects of the intervention described in Section 2.2 were investigated using a quasi-experimental pre-post-follow-up design with an experimental group (EG) who attended the intervention and a control group that did not participate in the course (baseline group (BG), see Figure 1). The latter group served to exclude pretest effects and capture effects resulting from participation in other courses or practical experiences. Pretest 1 with the affective questionnaire (see Section 2.4.2) took place directly before the start of the first course unit. The cognitive pretest (pretest 2; see Section 2.4.1) was carried out between the introductory session (unit 1) and the first session with station rotation learning (unit 2, see Figure 1) because the mind mapping in unit 1 should not be influenced by the questions of the knowledge test. The posttest (affective and cognitive test) was administered immediately after the completion of the last unit. Eight weeks after the end of the course the follow-up test (cognitive test) was performed.
There were two EG subgroups that participated in either a weekly or a block course format (see Figure 1, RQ 3 and [137]). To ensure comparability, the BG was also divided into two groups, who completed the questionnaire either at the beginning and end of the lecture period (and eight weeks later for the follow-up test; corresponding to weekly course attendance) or within the time of a block course during the semester break corresponding to block course participation.
Since many variables influence the impact of an intervention, each round of the intervention was always carried out by the same lecturer-accompanied by the same student assistant-in the same room. Therefore, the influence of these variables on the dependent variables is negligible. lecture period (and eight weeks later for the follow-up test; corresponding to weekly course attendance) or within the time of a block course during the semester break corresponding to block course participation.
Since many variables influence the impact of an intervention, each round of the intervention was always carried out by the same lecturer-accompanied by the same student assistant-in the same room. Therefore, the influence of these variables on the dependent variables is negligible.

Summary of the Intervention's Educational Concept and the Curricular Framework
A detailed description of the intervention with implementation instructions can be found in [171]. Therefore, the curricular framework, the target audience and elements of the course particularly relevant to this study are only briefly described here. For the reasons outlined in the introduction, one of the main goals of the intervention is to increase CK and ASCs not only with respect to one subject area of General Studies (e.g., biology), but to strengthen them in several subject areas. In this case, they are biology, chemistry, physics and technology.
The target audience includes pre-service primary teachers currently pursuing bachelor's and master's degrees-regardless of their major field of studies. The reason for admitting students from all kinds of disciplines is that most of them have to teach General Studies without having an appropriate college education [48]. Many participants (e.g., all master's students, bachelor's students specializing in English or social sciences) can only take the course voluntarily due to the existing examination regulations. The only group of students required to participate was bachelor primary teacher candidates majoring in science and technology (SciTec). This was done as part of a pilot project in which the intervention was applied as a substitute for a mandatory module component.
The entire intervention consists of twelve units (see Figure 1), each scheduled for 100 min. As mentioned before, students can either take a traditional, weekly format or attend

Summary of the Intervention's Educational Concept and the Curricular Framework
A detailed description of the intervention with implementation instructions can be found in [171]. Therefore, the curricular framework, the target audience and elements of the course particularly relevant to this study are only briefly described here. For the reasons outlined in the introduction, one of the main goals of the intervention is to increase CK and ASCs not only with respect to one subject area of General Studies (e.g., biology), but to strengthen them in several subject areas. In this case, they are biology, chemistry, physics and technology.
The target audience includes pre-service primary teachers currently pursuing bachelor's and master's degrees-regardless of their major field of studies. The reason for admitting students from all kinds of disciplines is that most of them have to teach General Studies without having an appropriate college education [48]. Many participants (e.g., all master's students, bachelor's students specializing in English or social sciences) can only take the course voluntarily due to the existing examination regulations. The only group of students required to participate was bachelor primary teacher candidates majoring in science and technology (SciTec). This was done as part of a pilot project in which the intervention was applied as a substitute for a mandatory module component.
The entire intervention consists of twelve units (see Figure 1), each scheduled for 100 min. As mentioned before, students can either take a traditional, weekly format or attend a four-day block course with three units per day. There is a maximum of 30 participants per intervention to ensure intensive support.
The course is divided into two thematic blocks of five units each, framed by an introductory and a concluding unit ( Figure 1). In thematic block 1, the context "the pond and its surrounding" is explored. In block 2, the context is "the human being and its physical performance". These contexts were chosen for the following reasons: 1. There are many connections to the children's experiences [3,172]. 2. Interdisciplinary interdependencies between the fields of biology, chemistry, physics and technology can be experienced. 3. Following the concept of exemplary in teaching [173], key concepts in science such as matter and energy [168] can be understood. 4. There are many points of reference to the guidelines of the curriculum for General Studies in North Rhine-Westphalia [174] and the recommendations of the Perspectives Framework for General Studies [3].
Based on the findings of previous studies [106,118], the learning environment was designed according to moderate constructivist learning theory [104,105]. Emphasis is placed on student-centered learning, which is supported by the lecturer. In three units per thematic block, participants acquire scientific and technical CK through a combination of station rotation learning and conducting experiments in partner work (see Figure 1). Learning at stations is preceded and followed by activities that are also relevant to this study:

1.
Before conducting the experiments: In the introductory session, the participants create a mind map for each of the two contexts noting existing CK on the subjects biology, chemistry, physics and technology. The creation and subsequent review of all mind maps prepared in partner work not only serve to activate prior knowledge but also help to recognize first contextual relationships and get an overview of the course topics [175]. Based on the mind maps' contents, the lecturer finally resolves which of the mentioned contents will be covered in the course.

2.
When conducting the experiments: Questions such as "What makes water lilies float on the surface of the water?" and "Why does a small stone sink?" form the starting point of the experimentation process. Considering that most pre-service primary teachers are more interested in biology than the other three subjects [176,177], many of the questions were formulated from a biological perspective to reduce the reservations many primary teachers have about the subjects of chemistry, physics and technology (see Section 1). The station worksheets are designed to develop biological, chemical, physical and technical CK through experimentation. First, hypotheses can be made about the outcome of the experiments. By conducting and interpreting experiments and accompanied by sharing ideas with the team partner, these hypotheses are verified or falsified. Thus, conceptual change [178] is enabled. During this work process, the participants should become aware that there is no division into individual subjects in nature, that many biological or technical phenomena can only be explained by chemical and physical laws and that chemistry [120], physics and technology have high relevance for everyday life [179,180].

3.
After conducting the experiments: a.
Background information: For each station a one-page text with further information on the scientific background of the experiment is available. This can be read as a refresher or reinforcement of CK if there is time after the station is completed and before the plenary debrief (see 3b). After each session, digital versions are available in the digital learning room, also containing bibliographical references to simplify further engagement into the topics. b.
Debrief: After each experimental work phase and in the final session, CK gained and interdisciplinary connections experienced are brought together and discussed in the plenary. This debrief is guided by the lecturer, supported by presentation slides with pictures from the experimentation phase and by giving impulses [175]. c.
Applying CK: In two additional units per topic block (see Figure 1), participants practice planning experiment-based science/technology lessons by applying and combining CK and PCK to create a child-oriented experiment.
In doing so, they can realize that CK is important for the development of PCK [69][70][71]. As part of this planning, CK that the teacher will need for this experiment is recorded in bullet points on a poster. The lecturer then provides written feedback to the groups on their planning via the digital learning space. Participants have access to feedback for all groups but do not know which people are behind which planning product to avoid social comparisons [112].

Sample
The study was conducted at a university in the federal state of North Rhine-Westphalia (Germany) from winter semester 2017/2018 up to and including summer semester 2019. During this period, the intervention took place eight times: Four times in a weekly and four times in a block course format. The sample included 238 primary teacher students, of whom 191 belonged to the experimental group (EG) while 47 formed the baseline group (BG).
Only the records of students who completed the questionnaire at all three measurement time points were subjected to data analysis (Section 2.5), resulting in a sample size of N total = 202 (N EG = 158; N BG = 44) with a mean age of 22.65 years (SD = 2.93 years). 79.2% of the students were in a bachelor's degree program, 20.8% were in a master's degree program. 90.1% of the participants were female. This gender distribution corresponds to the typical situation regarding the teaching staff at German primary schools (89.4% female teachers in the school year 2019/2020 [146]). 55.7% of the EG students attended the weekly, 44.3% the block course format (N EG, weekly = 88; N EG, block = 70). 47.7% (N BG, weekly = 21) of the BG-students completed the questionnaire at the beginning and end of the lecture period and eight weeks later, which corresponds to participation in the weekly course. The other 52.3% (N BG, block = 23) filled in the tests during the time span of a block course, which corresponds to block course attendance. 66.5% of the participants in the EG studied primary school education with a focus on science and technology (SciTec), the other 33.5% reported studying primary school teaching with other majors, e.g., English, social or religious studies. Therefore, when selecting participants for the BG, care was taken to ensure a similar distribution of the representatives in terms of the major field of study (N BG, SciTec = 27; i.e., 61.4%; N BG, no SciTec = 17; i.e., 38.6%). For data analysis and interpretation of results, it should be noted that 45.6% of the EG attended the course voluntarily (N EG, voluntary = 72), while 54.4% (N EG, mandatory = 86) participated as part of a mandatory module of their studies (see Section 2.2). Due to the examination regulations for elementary school teaching at the university, students without the major SciTec could only participate in the course voluntarily.

Instruments and Data Collection
Data were collected using the SoSci Survey online questionnaire tool [181]. Via the message forum of a digital learning space, information and links to the questionnaires, which included the cognitive and affective test separately, were provided for each reference time point. The test was administered as a power test [182] without time pressure since the intention was to investigate whether the students can answer the questions of the cognitive test correctly in a reasonable time frame. In addition, they were given the opportunity to reflect on their motivational orientations in detail and without pressure. However, participants were asked to complete the questionnaires without interruption and to answer the cognitive test without assistance.

Cognitive Test
The cognitive questionnaire assesses substantive, declarative CK in science and technology covering the intervention's topics (see Section 2.2 and [171], e.g., properties of water, human senses, bionics). Standards of the curriculum for General Studies in North Rhine-Westphalia [174] and recommendations of the Perspectives Framework for General Studies [3] were taken into account to ensure curricular validity. As with Ohle and colleagues [91], the curricular validity of the CK test was also guaranteed by analyzing textbooks from German primary schools, then from secondary schools and subsequently university-level textbooks and handouts for General Studies teachers. Table A1 (Appendix A) lists textbooks and handouts used for some of the topics covered in the test.
Due to the self-construction of the items, they were tested in a preliminary study with pre-service primary teachers and then revised. The final measurement instrument consists of 127 items in the form of 24 multiple-choice questions (each with three to eight items; multiple answer questions), three matching questions (each with three to four items) and three open-ended questions (eight items in total). Since closed-ended questions can be scored rapidly and facilitate the assessment of a large sample [183], they predominate in this questionnaire. According to Bridgeman and Lewis [184], closed-ended items do not necessarily have a lower validity than open-ended items. The order of the questions varied at each reference time to counteract order effects [185].
Based on the consensus that teachers' CK should extend beyond the school level in which it is taught [93] and Anders and colleagues' suggestion that primary school teachers' science CK should correspond at least to lower secondary level [94], all items represent secondary school level content. According to the classification by Ohle et al. [91,98] and textbook analysis (see Table A2), most items can also be assigned to primary school content level (26 items) and/or university content level (112 items). Following Ohle and colleagues [91,98] two levels of item complexity were used. Complexity level 1 includes facts (37 items), i.e., single facts and terms. Complexity level 2 covers relations and concepts (90 items), i.e., relating facts to each other, integrating concepts or applying (physical) laws.
To meet the interdisciplinary goals of the course, many questions relate to more than one discipline of General Studies, e.g., biology and chemistry or physics and technology. Sample questions can be found in Table A2. Multiple-choice items and matching items were scored with one point for a correct and zero points for an incorrect answer. Open-ended items were scored with zero to two points using a scoring horizon to ensure objective evaluation.
The criterion validity of the cognitive test was examined by correlating the pre-, posttest and follow-up test scores with the external criterion of students' last biology, chemistry and physics grades at secondary school, since the cognitive test includes topics that are also part of the curriculum in biology, chemistry and physics at this school level. Grades in technology were not correlated with the test results because this subject is not offered in many secondary schools. A correlation with the average grades in biology, chemistry, physics and technology during the study period was not performed as it turned out that many participants had not yet completed a science or technology course. Due to the interdisciplinary nature of the questions, no subdivision of the questions into purely biological, chemical, physical and technical questions was made for the calculation of the correlations (see also Section 4). Criterion validity is confirmed by weak but significant correlations for the domain of biology at all three times of measurement (pre: r biology = −0.

Affective Questionnaire
According to the classification of [116], this study examines CK-related ASCs. ASCs in science (biology, chemistry, physics) and technology were assessed using five-point Likert-type scales (1 = I strongly disagree to 5 = I strongly agree) and three items for each subscale (see Table 1; analogous wording for each subject).
An exploratory factor analysis (EFA; [186]) was carried out to test construct validity as the subscales and items listed in Table 1 were adapted from studies focusing on a different study group in combination with only one reference subject ( [187]: Subject: General Studies with focus on physics, study group: In-service primary teachers; [131]: Subject: Biology, study group: High school students). Kaiser-Meyer-Olkin (KMO) measure of sampling adequacy [188] indicated that the sampling was adequate for factor analysis as KMO values were >0.50 (pretest: 0.773; posttest: 0.790; [189]) and Bartlett's test of sphericity [190] was significant (p ≤ 0.001; [186]). A principal axis factor analysis with varimax rotation was performed for each time of measurement, including the twelve items shown in Table 1. Considering the theoretical assumptions [186] and the proportions of the total variances [189], a four-factor solution was chosen (see Table A3), which accounted for 75.04% (pretest) and 76.89% (posttest) of the variance, respectively. Items with the highest loadings on one common factor were combined into a subscale. Factor loadings of λ ≤ 0.50 were not accepted [191]. No item had to be removed as there were no crossloadings λ ≥ 0.40 [192] or highest loads on an unexpected factor. Factor loadings λ < 0.30, interpreted as low [193], are not shown in Table A3. Moreover, item communalities were calculated as they indicate the stability of the factor solutions. The average communalities h 2 of 0.75 (pretest) and 0.77 (posttest) are greater than 0.50 which is acceptable for sample sizes of approximately 200 persons [194]. Table 1. Affective variables (ASCs) with subscale name, number of items, the wording of the items in German (original) and English, item abbreviations, discriminatory power r it and reliability (Cronbach's α).

Subscales
Number

Data Analysis
Data analyses were carried out using the software SPSS statistics, version 27 (IBM Germany, Ehningen, Germany). Missing values in the data did not be replaced as the survey tool's function for reminding people to answer each item was set.
The cognitive test's open answers were evaluated using a scoring horizon to ensure objectivity. For correct scoring, incorrectly ticked items in the multiple-choice questions were recoded. Posttest items with a difficulty index of <20% (overly easy items) and >80% (overly difficult items) were eliminated [195] as the cognitive test aims to measure a person's cognitive performance. Ninety-eight items remained for further evaluation. Because the cognitive test examined broad scientific and technical CK, the next step was to exclude all items with discriminability values r it < 0.1 [130,195], resulting in 74 items with an internal consistency of Cronbach's α = 0.873 in the posttest (pretest: α = 0.721, follow-up test: α = 0.835). Based on George and Mallery's guidelines [196], these values of Cronbach's α are considered acceptable and good, respectively. With these 74 remaining items, a maximum of 81 points could be achieved at each reference time.
Since the ASCs examined here were operationalized by tight constructs, item discriminability values r it should be as high as possible [195]. For this reason, items with discriminability values r it < 0.3 with at least one of the two test times were excluded before further analyses were performed (values between 0.3-0.5 are considered medium, values above 0.5 as high [195]). As can be seen in Table 1, r it values for all items were well above 0.5 at both test times, so no item had to be removed. With Cronbach's α ≥ 0.8 and α ≥ 0.9 in the pre-and posttest, the reliabilities of the subscales can be regarded as good and excellent, respectively [196]. To allow comparison, total scores of the affective subscales were normed to a maximum of 5.
While most statistical tests are relatively robust to slight deviations from a Gaussian distribution [189], the total (normed) scores of all constructs were examined for normal distribution at all test times. Optical methods were also used as Kolmogorov-Smirnov and Shapiro-Wilk tests react very sensitively to minimal deviations from a normal distribution when in larger samples [189]. According to Q-Q plots, values of the skew index (−3.0 ≤ SI ≤ 3.0) and Fisher kurtosis (<8.0; [197]), data were normally distributed. Thus, parametric tests could be applied for cross-sectional and longitudinal comparisons. To exclude ceiling and floor effects [195], the pre-test means of all scales were examined for extremely high and extremely low values.
For longitudinal comparisons over two or three measurement times and between two groups, the following procedure-adapted from Damerau [131]-was chosen: Initially, independent samples t-tests [189] served to explore pretest differences between the two groups.
In case of no a priori differences, two-way repeated measures analyses of variance (ANOVAs) were performed to answer RQ 1 and 3, with the first factor being time (withinsubject) and the second factor being the main comparison group (between-subject). When interpreting the results, the interaction effect, i.e., the interaction between the factors, is decisive [198]. This interaction effect provides information on whether there is a significant difference in the development of the dependent variables between the two groups over time.
The influence of the variables "type of participation" (voluntary/mandatory) respectively "major field of studies" (SciTec/non-SciTec) was factored out by conducting three-way repeated measures ANOVAs with an additional between-subject factor representing the factored-out variable to be able to attribute possible changes in the dependent variables to the impact of the course format (RQ 3). Since the two factored-out factors are nominally scaled, they were included as between-subject factors rather than as covariates for which a metric scale level is required [191]. It was not possible to include both betweensubject factors simultaneously. The reason for this is the existing examination regulations, by which non-SciTecs can only participate voluntarily, resulting in a subgroup sample size of n non-SciTec+mandatory participation = 0.
In case of significant pretest differences between two groups, analyses of covariance (ANCOVAs; [191]) were applied with the pretest scores as the covariate, posttest or followup test scores as the dependent variable and one (RQ 1: Treatment; RQ 4: Major) or two (RQ 3; course format + the respective factored-out variable) independent variables as fixed factors. This setting partializes out the influence of the covariate and the second fixed factor (RQ 3) on the dependent variable. When interpreting the results, the main effect of the first fixed factor in each case (RQ 1: Treatment; RQ 3: Course format; RQ 4: Major) on the dependent variables is crucial. Interaction effects of fixed factor 1 and fixed factor 2 are also reported. These effects indicate that the interaction of both factors affects the knowledge gain or the development of the ASCs, respectively.
The following procedure was used to localize the previously identified differences: In the case of three measurement times (CK) one-way repeated measures ANOVAs were first conducted for each group to investigate whether knowledge changed significantly over time. If a significant change in knowledge was found, paired samples t-tests [189] were conducted to determine time spans in which significant changes occurred (pre-/posttest; pre-/follow-up test and post-/follow-up test). In the case of two measurement times (ASCs) paired samples t-tests were performed to examine pre-post differences within a group.
To answer RQ 3, the impact of the variables "type of participation" or "major field of studies" on the dependent variables again had to be factored out. Thus, instead of one-way repeated measures ANOVAs and paired samples t-tests, two-factor (pairwise) repeated measures ANOVAs [198] were employed. The variable "time" was used as within-subject factor and the factored-out variable ("type of participation" or "major field of studies") as between-subject factor. The main effect was considered to determine the impact of the factor "time" on the dependent variable [198].
Prior to performing the independent samples t-tests and ANCOVAs, Levene's tests were applied to assess the homogeneity of variances of the two populations. In case of unequal variances, Welch-tests were subsequently used [189]. Besides, before conducting repeated measures ANOVAs with three times of measurement, Mauchly's test of sphericity was used to test for equality of variances of differences. If the Mauchly's test was significant, correction procedures were applied (Greenhouse-Geisser epsilon ε > 0.75: Huynh-Feldt correction; ε < 0.75: Greenhouse-Geisser correction [199]).
To answer RQ 2, cognitive gain and changes in ASCs were first calculated by determining the differences between pre-and posttest for each participant. Then, Pearson r correlations were used to investigate the strength of the relationship [189] between cognitive gain and change in ASCs (Pearson correlation coefficient of |r| ≥ 0.1: Weak/small correlation; |r| ≥ 0.3: Moderate correlation; |r| ≥ 0.5: Strong/large correlation [201]). For the EG and BG, average cognitive test scores were examined for all three reference times. Table 2 shows corresponding means, standard deviations, p-values and effect sizes η p 2 for inner-and intergroup comparisons. Significance level: p ≤ 0.001 highly significant (***) [203]; effect size: 0.01 ≤ η p 2 < 0.06: Small effect, 0.06 ≤ η p 2 < 0.14: Medium effect, η p 2 ≥ 0.14: Large effect [201].

Results
According to the result of independent samples t-test, there is no significant difference in test scores between EG and BG at the time of pretest (t(200) = 0.114, p = 0.910, ω 2 ≤ 0.001). This means both groups had very similar declarative CK regarding the course contents prior to the intervention. At this point, both groups scored on average less than half of the maximum score of 81 points (M EG = 36.61; M BG = 36.45). Floor and ceiling effects [195] can therefore be excluded.
Due to the similarity of pretest means, a two-way repeated measures ANOVA was conducted to detect a significant group/treatment*reference time interaction. With Mauchly's test of sphericity being significant (Mauchly-W(2) = 0.848, p ≤ 0.001) and Greenhouse-Geisser epsilon ε > 0.75, a Huynh-Feldt correction was applied for calculation. The ANOVA shows that the EG is significantly different from BG in terms of cognitive gain across the three measurement time points (F(1.760, 351.902) = 79.644, p ≤ 0.001, η p 2 = 0.285); the effect size is large.
The following steps served to locate the observed differences: First, one-way repeated measures ANOVAs were conducted to investigate whether CK within EG and BG changed significantly over time. Since in the EG Mauchly's test of sphericity was significant (Mauchly-W(2) = 0.816, p ≤ 0.001) and Greenhouse-Geisser epsilon was ε > 0.75, the Huynh-Feldt correction was used: CK increased significantly over the three measurement times (F(1.706,267.782) = 343.798, p ≤ 0.001, η p 2 = 0.686). The large effect size indicates a statistically significant difference. On the other hand, there was no significant change in CK over time in the BG (F(2,86) = 1.997, p = 0.142, η p 2 = 0.044). Pretest effects or effects resulting from participation in other courses or practical experiences can thus be excluded.
Paired samples t-tests were then performed to localize the identified differences in the EG's test scores: CK increased significantly from pre-to posttest (t = −20.706, p ≤ 0.001, η p 2 = 0.740, n = 158) and from pre-to follow-up test (t = −20.545, p ≤ 0.001, η p 2 = 0.729, n = 158) with large effect sizes. However, it should also be noted that knowledge decreases significantly between post-and follow-up test, but the effect size is small (t = 2.834, p = 0.005, η p 2 = 0.049, n = 158). Overall, the results reveal both significant short-term and long-term gains of declarative CK for the participants of the intervention.

Academic Self-Concepts (RQ 1.2)
As shown in Table 3, the biology-related ASC is highest in both the EG (M = 3.74) and BG (M = 3.93) before the start of the intervention, followed by the technology-related ASC (M EG = 3.20; M BG = 3.19). The chemistry-and physics-related ASCs are rated the lowest (2.71 ≤ M ≤ 3.06). Especially for the last three subscales, floor-and ceiling effects [195] can be neglected as extremely high or extremely low mean values are not present.

Correlations between Cognitive Gain and Changes in ASCs (RQ 2)
Bivariate correlations were calculated between the change in CK and changes in ASCs

Impact of the Course Format
Having demonstrated that BG's CK and ASCs did not change significantly over time, we now examined whether the course format had an impact on cognitive gain and change in biology-, chemistry-, physics-and technology-related ASCs. Therefore, only the EG was considered for this purpose.
The impact of the factors "type of participation" (voluntary or mandatory) and "major field of studies" (SciTec or non-SciTec) was factored out as outlined in Section 2.5 to be able to attribute the effects to the influence of the course format. As described above, these factors had to be partialized out separately. Therefore, two statistical values are given for each scale and group. To improve the reading flow, the statistical values are not reported in the text. Instead, reference is made to the corresponding tables which contain the most important statistical characteristics.

Content Knowledge (RQ 3.1)
Since there were a priori differences in the test scores between weekly and block course participants, ANCOVAs were conducted for longitudinal comparisons between two groups with posttest/follow-up test scores as dependent variables, pretest scores as covariate and the variables "course format" and "type of participation"/"major field of study" as fixed factors. As shown in Table 4, there is a significant difference in the increase in CK from pre-to posttest between weekly and block course participants; the effect size is medium. Short-term cognitive gain is higher among block course participants. In terms of differences in long-term gains of cognitive knowledge, the following should be noted: A significant difference-with a small effect size-is only given if the influence of the type of participation is factored out. There is no difference when the impact of the major field of study is factored out (see Table 4). round brackets = AN(C)OVA with further between-subject factor "type of participation" (see Section 2.5); square brackets = AN(C)OVA with further between-subject factor "major field of study"; significance levels: p ≤ 0.05 significant (*), p ≤ 0.001 highly significant (***) [203]; effect size: 0.01 ≤ η p 2 < 0.06: Small effect, 0.06 ≤ η p 2 < 0.14: Medium effect, η p 2 ≥ 0.14: Large effect [201].
Significant interaction effects course format*type of participation or course format*major do not exist here, i.e., the factors "type of participation" or "major field of studies" do not additionally influence the effect of the course format on CK.
To locate the differences found, repeated measures ANOVAs were first performed for each group. The Huynh-Feldt correction was used for the subgroup of weekly participants as Mauchly's test of sphericity was significant and the Greenhouse-Geisser epsilon was ε > 0.75. According to the ANOVA's results, both the students who attended the weekly format and those who participated in the block course showed a significant change in cognitive performance over the three measurement times; the effect was large (Table 4). Further repeated measures ANOVAs, each including two measurement time points, were then conducted for each group to more precisely localize the identified differences in the average test scores. In both subgroups, there was a significant cognitive gain from pre-to posttest and from pre-to follow-up-test (large effect size). While CK decreased significantly from post-to follow-up test in the group of block course participants (large effect), there was no significant loss of knowledge in the group of weekly participants.

Academic Self-Concepts (RQ 3.2)
With no a priori differences between the two groups regarding the four subscales, three-way repeated measures ANOVAs were conducted to examine whether the ASC development of the two groups was significantly different (factor 1/within-subject-factor: Reference time; factor 2: Course format as between-subject factor; factor 3: The factored-out between-subject factor: "Type of participation" or "major field of study"). As can be seen in Table 5, there are no statistically significant differences in the change in ASCs between the groups.
Both weekly and block course participants showed significant increases in biology-, chemistry-, physics-and technology-related ASCs from pre-to posttest (Table 5). Effect sizes are large or medium, latter with respect to the biology-related and physics-related ASC of weekly participants.
It can be concluded that both course formats lead equally to a positive change in biology-, chemistry-, physics-and technology-related ASCs of pre-service primary teachers.
No significant interaction effects time*course format*type of participation or time* course format*major field of study were observed. I.e., the factors "type of participation" or rather "major field of study" do not additionally influence the effect of the interaction time*course format on the dependent variables. The only exception is the technologyrelated ASC when factoring out the impact of the type of participation. There is a significant interaction effect (F(1,154) = 4.359, p = 0.038, η p 2 = 0.028), but the effect size can be described as small. Table 5. Means (M) and standard deviations (SD) of biology-, chemistry-, physics-and technology-related ASCs at both measurement times comparing the groups of weekly (n = 88) and block format (n = 70) participants. p-values and effect sizes η p 2 are given for inner-and intergroup comparison. round brackets = ANOVA with further between-subject factor "type of participation" (see Section 2.5); square brackets = ANOVA with further between-subject factor "major field of study"; significance level: p ≤ 0.001 highly significant (***) [203]; effect size: 0.01 ≤ η p 2 < 0.06: Small effect, 0.06 ≤ η p 2 < 0.14: Medium effect, η p 2 ≥ 0.14: Large effect [201].

Impact of the Major Field of Study
The intervention aims to develop the professional skills of all pre-service primary teachers, regardless of their major field of study. Thus, we also examined whether there are significant differences in the development of CK and ASCs between SciTec and non-SciTec students within the groups of participants in the weekly and block format. It was not possible to factor out the impact of the type of participation since there was a subgroup sample size of n non-SciTec+mandatory participation = 0 due to examination regulations.
In the case of the block course subgroups, the two-way repeated measures ANOVA indicates a significant difference in the development of CK between SciTec-and non-SciTec students, with a medium effect size (F(2,136) = 5.610, p = 0.005, η p 2 = 0.076; Table 6). Further pairwise two-way repeated ANOVAs, each with two measurement time points, served to localize the difference found. There is a significant difference in cognitive gain between preand posttest (F(1,68) = 9.805, p = 0.003, η p 2 = 0.126) and between pre-and follow-up test (F(1,68) = 5.371, p = 0.023, η p 2 = 0.073). The effect size is medium. Thus, within the group of block course participants, short-and long-term gain of CK is greater among non-SciTec pre-service teachers ( Table 6). Table 6. Means (M) and standard deviations (SD) of declarative CK at all three measurement times comparing weekly participants with a SciTec major (n = 60) and a different major (n = 28) and block format participants with a SciTec major (n = 45) and a different major (n = 25). p-values and effect sizes η p 2 are given for inner-and intergroup comparison. Significance levels: p ≤ 0.01 very significant (**), p ≤ 0.001 highly significant (***) [203]; effect size: 0.01 ≤ η p 2 < 0.06: Small effect, 0.06 ≤ η p 2 < 0.14: Medium effect, η p 2 ≥ 0.14: Large effect [201].
The results of the analyses show no significant differences in the development of the ASCs between the subgroups (Table 7). This means that both groups of pre-service primary teachers benefited equally from taking the course in terms of ASC development. With one exception, paired samples t-tests revealed significant increases in biology-(SciTec weekly : There is no statistically significant change in the biology-related ASC from pre-to posttest in the group of non-SciTec pre-service teachers, who took part in the weekly course format (t = −1.675; p = 0.106, n = 28; Table 7). Table 7. Means (M) and standard deviations (SD) of biology-, chemistry-, physics-and technology-related ASCs at both measurement times comparing weekly participants with a SciTec major (n = 60) and a different major (n = 28) and participants of the block format with a SciTec major (n = 45) and a different major (n = 25). p-values and effect sizes η p 2 are given for inner-and intergroup comparison.  Significance levels: p ≤ 0.05 significant (*), p ≤ 0.01 very significant (**), p ≤ 0.001 highly significant (***) [203]; effect size: 0.01 ≤ η p 2 < 0.06: Small effect, 0.06 ≤ η p 2 < 0.14: Medium effect, η p 2 ≥ 0.14: Large effect [201].

Discussion
In this study, the impact of an interdisciplinary university course on pre-service primary school teachers' CK and ASCs in science and technology has been examined. This is intended to contribute to providing learning environments that inherently hold the potential to foster professional knowledge and motivational orientations concerning multiple disciplines-in this case, biology, chemistry, physics and technology-simultaneously. Not only does this take into account the interdisciplinary nature of General Studies [3], but it may also be a possible solution to making the most profitable use of the limited time often allotted to this broad subject in university teacher education [43,44].

Cognitive Gain
Consistent with previous studies [22][23][24][25][26][27][28] the pretest results indicate rather low declarative CK of pre-service primary teachers in science and technology. Regarding the relevance of teachers' CK for the development of PCK and the teaching activities [55,58,68] as well as the difficulty level of the items, a dilemma of primary teacher education becomes apparent. Theoretically, all questions of the test could be answered correctly by the participants already in the pretest, since they correspond to the secondary school level according to the analysis of the textbooks (see Section 2.4.1). Since this is not the case, it can be assumed that some university courses suppose basic CK that is not available at all. How can possible alternative conceptions of children be analyzed and discussed in a seminar if the pre-service teachers do not have the CK to even recognize these misconceptions? Do lecturers go over this and directly teach university-level CK, or do they invest time to help pre-service teachers refresh CK at secondary school level, which is-according to [94]-the level of CK that they will need for their professional practice? The latter objective is pursued by the present intervention.
In contrast to the BG, there are short-and long-term gains in the EG in terms of substantive, declarative CK in science and technology (RQ 1.1). Course design took into account previous findings on learning environments that have led to gain in CK, such as content focus [102,103] and a moderate constructivist setting [106]. Making assumptions before conducting the experiment or mind-mapping at the beginning of the course activate prior knowledge that can be referred to during the course and reflected upon through the activities. Follow-up studies could investigate, among other things, whether omitting mind-mapping results in even lower CK scores in the pretest. Other factors that may have led to an increase in knowledge include: Study materials to refresh or reinforce CK (one-page texts), debrief in plenary to clarify ambiguities or questions and application of CK in planning experiment-based science/technology lessons (see Section 2.2).
Due to the interdisciplinary nature of many questions, a division according to the four disciplines was dispensed with in the evaluation. Similar to the study by Niermann [144], a test could also be used in the future in which the items can be clearly assigned to a discipline.
However, this would not meet the interdisciplinary course concept and the requirements regarding an interdisciplinary mindset of General Studies teachers [13]. Another, more open way to investigate cross-linked CK would be the concept mapping method, which was used in the study of [102]. The validity of the knowledge test and the generalizability of the results are discussed in Section 4.4., also with the help of the interpretation of the results for RQ 2.

Science-and Technology-Related Academic Self-Concepts
So far, it has been an open question whether pre-service primary teachers differentiate between different domains of science (biology, chemistry, physics) respectively General Studies (science, technology, history, geography and politics) in their ASCs. For school context, it has been found that pupils differentiate between related (sub-)domains and content areas within a subject [121,204,205]. Gabriel-Busse et al. [121] showed that, despite multi-perspective teaching practice of General Studies, German primary school children develop five subject-specific ASCs (science, technology, history, geography and politics) that can be empirically distinguished from one another. Science domain-specific ASCs were not found. According to the results of the factor analysis, participants of this study do not have a "general" scientific-technical ASC or, for example, a science ASC and a technology ASC. Instead, they distinguish between their abilities in biology, chemistry, physics and technology, both in the pre-and posttest. These findings could result from the fact that in most German secondary schools, these four subjects are traditionally taught separately and not interdisciplinarily as in other countries [205]. Moreover, many General Studies courses at university only relate to one of these subjects [43]. Different performance experiences in these subjects lead to a differentiation of ASCs, with contrast effects also playing a role [206]. As in previous studies [32,33], the biology-related ASC is rated highest, whereas the chemistry-and physics-related ASC is rated lowest.
Factor cross-loadings of λ ≥ 0.30 arising in the rotated factor matrix of the posttest data for factor 3 (physics-related ASC) and factor 4 (technology-related ASC; see Table A3) might be an indication that participating in an integrative educational setting leads to change in the ASC structure. The intervention emphasizes connections between the disciplines and focuses on interdisciplinary competencies such as problem-solving skills needed for experimentation (Section 2.2). In an interdisciplinary setting, contrast effects and the use of dimensional reference norms [114,134,206] may therefore play a minor role in affecting the ASC. Jansen et al. [204] also found that the learning environment can influence ASC structures: Correlations between the three ASC subscales biology, chemistry and physics were significantly higher in the group of ninth-grade students who were taught science in an integrated subject for several years, compared to students who were taught the three sciences separately. Subsequent longitudinal studies should therefore investigate the structural stability [110] of pre-service primary teachers' ASCs. Moving toward a "general scientific-technical ASC" by attending several interdisciplinary courses may be evidence of more coherent thinking. This would meet both the goals of General Studies, being an integrative subject [3,45], and the recommendations recorded in the qualification model concerning teacher education for General Studies [13].
The results of our study indicate that a single course has the potential to reinforce pre-service primary teachers' ASC in multiple science and technology domains (RQ 1.2). While in the EG, ASC increased significantly in all four subscales from pre-to posttest with high effect sizes, there were no changes in ASCs in the BG. Comparing the change in ASCs between the two groups, the difference is significant, but the effect sizes are small to moderate. This may be due to the unequal size of the comparison groups since this can affect effect size [195]. A follow-up study with a larger sample, especially with regard to the BG, should therefore investigate whether this positive trend is confirmed. If follow-up studies confirm these small to moderate effects, the results should lead to the conclusion that such one-semester intervention does indeed have a small or moderate impact on ASC development (although not a large effect).
Looking at the results of previous studies, it can be assumed that the interdisciplinary character of the intervention plays an essential role in the positive development of ASCs: While in the work of [119,147] physics-related and technology-related ASCs, respectively, did not change significantly through courses that addressed only one domain at a time, pre-service teachers' science-related ASCs increased during a program of study with several modules integrating science [32]. In Janssen's study [120], assessing the effects of a one-semester seminar integrating biology and chemistry, the chemistry-related ASC of pre-service primary teachers also developed positively. Future studies should investigate whether participants' domain-specific ASCs remain stable at the posttest level over the course of the study, similar to [32], or whether they change due to the influence of other courses or practical experience at school.
The following course activities or components may have resulted in the positive development of participants' ASCs: By independently acquiring CK through experimentation and applying professional knowledge when planning lessons, they can experience their competencies and gain positive learning experiences [108,110,135]. A positive, supportive learning atmosphere, mentioned by participants in the open-ended responses [171], is also cited in the literature as a factor influencing self-concept [110]. Verbal feedback during the work phases, written feedback on the planning products [135,136] and the debriefing phases promote reflection on own competencies. These continuous feedback structures and the comparison with requirements, such as the learning objectives of the course (criterial reference norm, [110]), help to assess one's state of performance. Using temporal comparisons [110,133], participants may find that they have more science and technology CK at the end of the course than at the beginning. The influence of grades, which are also frequently used as a source for performance evaluation [123,207], can be excluded since these were not assigned during the survey period. In addition, by anonymizing the written feedback by the lecturer and setting objective feedback criteria, we tried to minimize the negative effects of social comparisons [16,112,133].

Impact of the Course Format
This study contributes to filling the research gaps in the field of higher education regarding the impact of course format (RQ 3) on students' development of professional competencies [153].
Our findings are in line with the majority of previous research showing that block courses lead to equal or even better knowledge gains than weekly courses [155][156][157]159,160]. The significantly higher short-term cognitive scores of the block course participants could be related to the fact that the course was not interrupted by other courses due to its format and position in the lecture-free period. This can lead to less stress [208], a more positive learning atmosphere [209], a higher level of concentration on the learning content, a more continuous learning experience and a more intense and more efficient mental linking of the contents in comparison to the weekly format [148,158,210,211].
However, it is more important to examine the differences in terms of long-term CK retention, as "retention is considered the more serious test because it shows which knowledge is retained or stable over longer time periods" ( [170], p. 21). If it turns out that block course participants lose a lot of CK from the course after six months, it is questionable to offer this course format, as it usually still takes time for them to apply the knowledge in professional practice. Again, there are slight advantages for block course participants, but only with small effect sizes and only when the type of participation is factored out. Future studies need to investigate whether this outcome is also observed when the variables "type of participation" and "major field of study" are simultaneously partialized out. In this study, this was not possible due to existing examination regulations, according to which non-SciTec students cannot be obliged to attend the course.
Besides, it is striking that among the weekly group, the CK remained stable from the post to the follow-up test, while the knowledge of the block course participants decreased significantly. In terms of long-term knowledge gain, participants of the weekly format could benefit from learning less content at once, which they can process and deepen gradually over weeks [148,165,166]. Follow-up studies covering a longer observation period than two months after the end of the course could provide further evidence for this.
Similar to Bateson's results [167], block course participants have an advantage in shortterm CK gain, but there are no differences between the groups in ASC change between the pretest and posttest. Both course formats are equally beneficial to the positive development of science-and technology-related ASCs. Thus, the period over which a course runs (a semester, i.e., several months, as opposed to four days) does not appear to be a determining factor in the development of pre-service teachers' ASCs in this case. The trends observed in the studies by [154,159] (see Section 1.3) are not supported by our findings.

Impact of the Major Field of Study
One of the important things to consider when examining the effects of different course formats is the pre-existing knowledge of the participants, as this can have an impact on learning success [151]. Based on previous studies, showing that primary teachers with a science background have a higher science CK than those without a science major [22,45], it was assumed that non-SciTec students would have lower science and technology CK than SciTec students at pretest. This has been confirmed only for the group of weekly participants. In the block format group, CK of the non-SciTecs was also lower in the pretest but did not differ significantly from that of the SciTecs. One possible reason for these differences could be Burton and Nesbit's [151] finding that students who are more confident in a subject are more likely to choose the block format. This also matches the results that the pretest ASCs of the weekly non-SciTec participants were significantly lower than those of the SciTec students in three subscales, whereas this was the case in the block format group in only one subscale (see Section 3.4.2). Burton and Nesbit's thesis, however, is refuted when the pretest CK scores of the weekly and the block course participants are considered more globally. The scores of both SciTecs and non-SciTecs who attend weekly are each comparatively higher than those of block course participants. In future studies, the number of participants per subgroup should be as similar and as large as possible because unequal study group sizes and sample size affect test power and effect size [195].
Overall, while controlling for pretest scores using ANCOVAs when necessary, both pre-service teachers with and without a SciTec major benefit from the course in terms of CK and ASC, regardless of whether they attend weekly or the block course (RQ 4). Regarding the ASC, the intervention seems to include situations and factors that lead to a positive development of science-and technology-related ASC for both groups of pre-service teachers (see Section 4.1.2). With a heterogeneous learning group and an instructional setting that thrives on interaction with one another (see Section 2.2), social comparisons [16,112,117,133] are also expected to have an impact on ASC. In addition to the aforementioned attempt to avoid the negative effects of social comparisons, non-SciTecs may have found that their knowledge and skills in science and technology are similar when compared to SciTec students.
In line with the findings of [137,138], however, the comparison of short-and long-term CK gains within the block course group also demonstrates that the major field of study can be a factor influencing ASC development. The combination of a course format that has hitherto been little represented in university teaching with learning content from areas that are rarely or completely untouched in one's studies may be one of the reasons why non-SciTecs show a significantly higher increase in knowledge.

Limitations and Implications for Further Research
Due to the existing examination regulations, many of the pre-service teachers attended the course voluntarily. It can be assumed that mainly those interested in such training participated in the intervention. According to Richter [212], teachers who already have a higher CK level are more likely to attend professional training. Participants could also choose the course format they prefer. Thus, in subsequent studies, randomized assignment of pre-service teachers to the groups (EG/BG and weekly/block) would be appropriate. Otherwise, differences in the outcomes could already be due to self-selection. However, such an experimental design is often difficult to implement in regular university teaching under the given examination regulations. Burton and Nesbit also point out that "random assignment of students to traditional or block classes is unlikely to be acceptable to students, ethics committees or to universities, given the normal practice of allowing students to choose between available alternatives" ( [151], p. 6).
As mentioned above, in addition to increasing the sample size per subgroup, the stability of CK and ASC over a longer period than two months after the end of the course should be examined to better assess the sustainability of the intervention.
Regarding the cognitive questionnaire, the following limitations should be noted: CK is domain-specific [213]. That is, a high CK in one topic (e.g., human respiratory system) does not necessarily imply high CK in another topic (e.g., human senses). Even though the questionnaire covers several topics from science and technology, only course-specific content is assessed, which limits the generalizability of the results. The same applies to the type of knowledge. In future studies, not only the development of declarative CK but also that of procedural or situational CK could be investigated, as these are also relevant for professional action [54]. In preparing the analysis of the cognitive test, we were also confronted with a reliability-validity trade-off [214]: A broad coverage of the knowledge under investigation usually increases validity, but this may lead to low reliability of the test. We tried to find a balance by using a test that represents a wide variety of topics and by choosing a low discriminability value that led to the exclusion of items, as is recommended for broad constructs [195]. Nevertheless, several items had to be removed, which on the one hand led to acceptable reliability (see Section 2.5), but on the other hand, may result in lower validity. Referring to the findings of Paulick and colleagues [116], our study tested the validity of the knowledge test by correlating CK increases with changes in ASCs (RQ 2). The more the participants gained CK, the more their physics-and technology-related self-concept increased. No significant correlations were found for the biology-and chemistry-related ASCs. These results can be interpreted to the effect that the questionnaire-after removal of the items according to the above criteria-adequately covers the CK in the disciplines of physics and technology and that there is further need for optimization for the disciplines of biology and chemistry. This need is alleviated when the results on correlations with the external criterion "last school grade" are added. Here, weak but significant correlations were found between CK posttest scores and school grades in biology, chemistry and physics. As with [72], high school GPA (grade point average) could additionally be used for external testing of knowledge test's validity in the future.
As an online questionnaire was used, it cannot be ruled out that participants relied on external help to answer the questions, even if they were asked not to do so. Due to the anonymity of the test and the lack of an examination character, it was not expected that external help would be used. The instrument "online questionnaire" was deliberately chosen because it offers many advantages: There are more ways to present the questions, resource consumption is less, and with open-ended answers, there is no difficulty deciphering the handwriting. Missing values are reduced because participants are alerted to missing responses, and data entry time and errors are minimized as data no longer need to be entered manually by the person conducting the evaluation. The primary reason, though, was that it would otherwise have been difficult to reach participants for the follow-up test two months after the end of the course.
In this study, the instructor of the intervention was also the researcher. The influence of this confounding variable [215] was minimized by considering several aspects of course delivery, data collection and data analysis: The effect of "teaching to the test" was avoided by the course setting, which emphasized self-directed knowledge acquisition through experimentation, and by the abundance of supplementary material provided after the experiments (see Section 2.2). To ensure objectivity in conducting the study [195], the links to the online questionnaires were always sent by the same test administrator with the same instructions and information via the digital message forum in all course runs. Objectivity of data analysis was ensured by using a predominantly closed-response format, a scoring key for the open-ended responses in the cognitive test and a standardized data analysis procedure (see Section 2.5). Since CK alone is not sufficient ensure to lead to effective instruction or to improve pupil achievement [60,216] future studies should also consider the intervention's impact on pre-service teachers' PCK. It could be explored, for example, whether attending the course reinforces pre-service teachers' PCK about primary school children's (alternative) conceptions of course topics. Referring to the finding that CK is negatively correlated with PCK self-concept [117], we could also examine whether gains in CK lead pre-service teachers to have more pessimistic perceptions of their PCK.

Conclusions
In summary, this study shows that a single interdisciplinary university course holds the potential to positively impact not only pre-service primary teachers' CK but also their ASC in several disciplines. Thus, interdisciplinary interventions of this kind could be one way of addressing the challenges facing the primary teacher education system worldwide (see introduction). The course concept has been proven to be suitable for a heterogeneous learner population. The finding that the course format can significantly influence the CK is relevant not only to the design of university curricula and courses but also to the offering of voluntary professional development courses in teacher education.
Author Contributions: Conceptualization, research design, methodology, data collection, validation, data analysis, visualization, writing-original draft, funding acquisition, M.M.B.; supervision, data analysis, writing-review and editing, K.D.; supervision, conceptualization and funding acquisition, A.P. All authors have read and agreed to the published version of the manuscript.
Funding: This work was funded by the "Qualitätsoffensive Lehrerbildung", a joint initiative of the Federal Government and the Länder, which aims to improve the quality of teacher training. The program is funded by the Federal Ministry of Education and Research (BMBF). The authors are responsible for the content of this publication; grant number 0JA1507. We gratefully acknowledge the support of the Open Access Publication Fund of the University of Wuppertal (grant number: GoldOA-PUBL20210923).
Institutional Review Board Statement: Ethical review and approval were waived for this study because, according to the German legislation on research involving human subjects, ethical approval is only required when sensitive data are collected, when physical interventions are performed, or when subjects could be harmed. Before the start of the study, all participants were informed of the aims of the study, that participation was voluntary, that by completing the questionnaire they were giving their consent to participate voluntarily, that participation could be discontinued at any time, and that full anonymity was guaranteed.
Informed Consent Statement: Informed consent was obtained from all subjects involved in the study.

Data Availability Statement:
The data presented in this study are available on request from the corresponding author.

Conflicts of Interest:
The authors declare no conflict of interest. The funders had no role in designing the study, in data collection, in analyzing and interpreting the data, or in writing the manuscript. Table A1. Examples of textbooks and handouts used to ensure the curricular validity of the cognitive questionnaire.