Effect of Daytime and Tree Canopy Height on Sampling of Cacopsylla melanoneura, a ‘Candidatus Phytoplasma mali’ Vector

The psyllids Cacopsylla melanoneura and Cacopsylla picta reproduce on apple (Malus × domestica) and transmit the bacterium ‘Candidatus Phytoplasma mali’, the causative agent of apple proliferation. Adult psyllids were collected by the beating-tray method from lower and upper parts of the apple tree canopy in the morning and in the afternoon. There was a trend of catching more emigrant adults of C. melanoneura in the morning and in the lower part of the canopy. For C. melanoneura remigrants, no differences were observed. The findings regarding the distribution of adults were reflected by the number of nymphs collected by wash-down sampling. The density of C. picta was too low for a statistical analysis. The vector monitoring and how it is commonly performed, is suitable for estimating densities of C. melanoneura. Nevertheless, above a certain temperature threshold, prediction of C. melanoneura density might be skewed. No evidence was found that other relatively abundant psyllid species in the orchard, viz. Baeopelma colorata, Cacopsylla breviantennata, Cacopsylla brunneipennis, Cacopsylla pruni and Trioza urticae, were involved in ‘Candidatus Phytoplasma mali’ transmission. The results of our study contribute to an advanced understanding of insect vector behavior and thus have a practical impact for an improved field monitoring.


Introduction
Monitoring schemes for herbivore insects strongly depend on detailed knowledge about their specific requirements for natural resources, including food, mates or oviposition sites [1]. For arboricolous taxa in particular, knowledge about their distribution on different parts of a tree is also considered important to optimize sampling strategies for a meaningful density estimation [2].
The psyllids Cacopsylla melanoneura (Foerster, 1848) and C. picta (Foerster, 1848) (Hemiptera: Psylloidea: Psyllidae) are phloem feeding insects native to the Palaearctic region and associated with cultivated apple (Malus × domestica). While C. melanoneura is oligophagous on hawthorn (Crataegus spp.), apple (Malus spp.), medlar (Mespilus germanica) and pear (Pyrus communis), the only host plant of C. picta is apple [3,4]. Both species are univoltine and, similar to a number of other taxa of Psylloidea in the north temperate zone, they alternate between host and shelter plants during their life cycles [5,6]. Conifers serve as shelter plants for overwintering adults [7,8], while during the spring and summer C. melanoneura and C. picta spend four to five months on apple trees where they reproduce and temperatures [34]. Attraction to light has been demonstrated experimentally for some species of Psylloidea, e.g., Diaphorina citri (Kuwayama, 1908) [36] and the carrot psyllid, Trioza apicalis (Foerster, 1848) [37]. Under laboratory conditions, carrot psyllids preferred carrot leaves placed at higher light intensities [37]. Under field conditions, trap catches of the carrot psyllids and sunlight hours correlated stronger in autumn (when air temperatures were low) than in summer, indicating an interaction effect between temperature and light intensity [38].
Due to pragmatic reasons, AP vector monitoring with beating trays is commonly performed in the lower canopy (ca. 1-1.5 m above the ground) and throughout the entire day. Despite the high economic impact of C. melanoneura and C. picta, little is known about the small-scale spatio-diurnal dynamics of the AP-vector insects on apple trees. Thus, the following questions were addressed in the present study: (i) does the daytime (and the associated temperature) influence the number of AP vector catches? and (ii) is there any difference in the distribution of AP vectors at different canopy heights of apple trees? Based on the previously mentioned information on psyllid biology and behavior, we predicted to catch significantly more specimens of AP vectors in the morning and in the upper canopy. Furthermore, to analyze if other psyllid taxa were potentially involved in 'Ca. P. mali' transmission, quantitative PCR was performed to detect this pathogen in individuals of different Psylloidea species abundantly found in the orchard under study.

Temperatures in the Apple Orchard
Complete data on temperatures in the morning and afternoon are shown for each two calendar weeks (cw) in Appendix A Table A1. The temperatures varied in the three-year sampling period. In particular, the average temperatures in the morning and in the afternoon were higher from April to June 2015 compared to the subsequent two years ( Figure 1). This time frame corresponds to the presence of C. melanoneura emigrants.
Plants 2020, 9, x FOR PEER REVIEW 3 of 15 recolonize the tree after a short time [35]. Stratopoulou and Kapatos also found that C. pyri prefers to populate the southern and western sides of pear trees, which may be due to higher light intensities and temperatures [34]. Attraction to light has been demonstrated experimentally for some species of Psylloidea, e.g., Diaphorina citri (Kuwayama, 1908) [36] and the carrot psyllid, Trioza apicalis (Foerster, 1848) [37]. Under laboratory conditions, carrot psyllids preferred carrot leaves placed at higher light intensities [37]. Under field conditions, trap catches of the carrot psyllids and sunlight hours correlated stronger in autumn (when air temperatures were low) than in summer, indicating an interaction effect between temperature and light intensity [38]. Due to pragmatic reasons, AP vector monitoring with beating trays is commonly performed in the lower canopy (ca. 1-1.5 m above the ground) and throughout the entire day. Despite the high economic impact of C. melanoneura and C. picta, little is known about the small-scale spatio-diurnal dynamics of the AP-vector insects on apple trees. Thus, the following questions were addressed in the present study: (i) does the daytime (and the associated temperature) influence the number of AP vector catches? and (ii) is there any difference in the distribution of AP vectors at different canopy heights of apple trees? Based on the previously mentioned information on psyllid biology and behavior, we predicted to catch significantly more specimens of AP vectors in the morning and in the upper canopy. Furthermore, to analyze if other psyllid taxa were potentially involved in 'Ca. P. mali' transmission, quantitative PCR was performed to detect this pathogen in individuals of different Psylloidea species abundantly found in the orchard under study.

Temperatures in the Apple Orchard
Complete data on temperatures in the morning and afternoon are shown for each two calendar weeks (cw) in Appendix A Table A1. The temperatures varied in the three-year sampling period. In particular, the average temperatures in the morning and in the afternoon were higher from April to June 2015 compared to the subsequent two years ( Figure 1). This time frame corresponds to the presence of C. melanoneura emigrants.

Psyllid Community
In total, 1021 specimens belonging to 28 species of Psylloidea were collected in the orchard during the three seasons of the study (Table 1). C. melanoneura was the most abundant species with a total of 630 specimens. C. picta was only found sporadically with a total of 13 specimens. Other Psylloidea species that were at least as abundant in the orchard as C. picta but do not reproduce on

Psyllid Community
In total, 1021 specimens belonging to 28 species of Psylloidea were collected in the orchard during the three seasons of the study (Table 1). C. melanoneura was the most abundant species with a total of 630 specimens. C. picta was only found sporadically with a total of 13 specimens. Other Psylloidea species that were at least as abundant in the orchard as C. picta but do not reproduce on apple, were Baeopelma colorata (Löw, 1888), C. pruni (Scopoli, 1763), C. brunneipennis (Edwards, 1896), Trioza urticae (Linnaeus, 1758) and C. breviantennata (Flor, 1861).

Daytime Variation in the Numbers of C. melanoneura Specimens
Remigrants of C. melanoneura, which are recognizable by their prevailingly dark brown body, were detected from the beginning of March to the middle of April in 2015, from the middle of February to mid-April in 2016, and from mid-February until the end of March/beginning of April in 2017. In all three years, the light-green colored C. melanoneura emigrants were collected on apple trees from the end of April until the middle/end of May ( Figure 2).
In March and May 2015, more C. melanoneura individuals were sampled in the morning (Figure 2a). Considering the total numbers of catches throughout the year, significantly more emigrants were collected in the morning than in the afternoon in 2015 (Figure 2b). In 2016 and 2017, C. melanoneura catches were similar in the morning and afternoon throughout the whole sampling period (Figure 2a) and only a slight trend of catching more emigrants in the morning was observed in these two years ( Figure 2b). However, these differences were not statistically significant.
The probabilities to catch the same numbers of specimens in the morning as in the afternoon were similar when temperatures were below 8.7 • C in the morning or below 17.6 • C in the afternoon, respectively (Figure 3a,b). When temperatures exceeded these thresholds, more specimens were caught in the morning and less in the afternoon. Temperatures in the morning correlated positively with with temperatures in the afternoon ( Figure A1), i.e., if the temperatures exceeded 8.7° C in the morning they were above 17.6° C in the afternoon.  Probability of catching C. melanoneura specimens depending on the temperature (a) in the morning (7-9 am); (b) in the afternoon (2-4 pm). Analysis was conducted to establish whether the probability of catching more C. melanoneura individuals is dependent on the temperature in the morning or in the afternoon. The 50% threshold (y-axis) indicates equal catch probabilities between morning and with temperatures in the afternoon ( Figure A1), i.e., if the temperatures exceeded 8.7° C in the morning they were above 17.6° C in the afternoon.  Probability of catching C. melanoneura specimens depending on the temperature (a) in the morning (7-9 am); (b) in the afternoon (2-4 pm). Analysis was conducted to establish whether the probability of catching more C. melanoneura individuals is dependent on the temperature in the morning or in the afternoon. The 50% threshold (y-axis) indicates equal catch probabilities between morning and . Analysis was conducted to establish whether the probability of catching more C. melanoneura individuals is dependent on the temperature in the morning or in the afternoon. The 50% threshold (y-axis) indicates equal catch probabilities between morning and afternoon. If the temperature was above 8.7 • C in the morning, the catch probability was higher (>50%) in the morning (a). If the temperature in the afternoon was below 17.6 • C, the catch probability was similar in the morning and in the afternoon, respectively (b).

Variation of C. melanoneura Densities at Different Canopy Heights
The sampling on apple trees was carried out at two different canopy heights, i.e., at 1 m and 2.5 m above the ground. In 2015, C. melanoneura catches from the two heights did not differ over the season (Figure 4a) and the totals of C. melanoneura remigrant and emigrant specimens were almost equal (Figure 4b). In 2016, similar numbers of C. melanoneura specimens were collected at the upper and lower canopy, except for May when the number of emigrants peaked and significantly more specimens were found in the lower canopy ( Figure 4a). Furthermore, the total number of C. melanoneura remigrants was significantly higher in 2016 in the upper canopy, while significantly more C. melanoneura emigrants were collected in the lower canopy (Figure 4b). In 2017, the scenario was similar to 2016: i.e., in May significantly more individuals were caught in the lower canopy, while throughout the rest of the season between February and June, no differences were observed (Figure 4a). Over the whole season in 2017, significantly more emigrants were also found in the lower canopy (Figure 4b). The numbers of remigrant specimens did not differ between the two canopy heights in 2017 (Figure 4b).   The distribution of C. melanoneura nymphs at different canopy heights was analyzed in a single year (2016) ( Figure 5). The nymphs occurred from the end of March/beginning of April, around six weeks after the arrival of the C. melanoneura remigrants and could be detected until mid-May, when C. melanoneura emigrant population peaked. During this period, similar numbers of nymphs were collected at the upper and lower canopy (Figure 5a), although the total number of nymphs per year was significantly higher in the upper canopy (Figure 5b).

Sampling of C. picta
Altogether, only 13 individuals of C. picta were collected in the three years of the study. This low number did not allow any statistical evaluation of the effects of daytime and canopy height on

Sampling of C. picta
Altogether, only 13 individuals of C. picta were collected in the three years of the study. This low number did not allow any statistical evaluation of the effects of daytime and canopy height on sampling. In 2015 and 2016, C. picta remigrants were recorded from April until the beginning of May. One C. picta emigrant was collected in the beginning of June 2015 and one in the beginning of July 2016, respectively. In 2017, no C. picta specimens were found.

Infection Rate with 'Ca. P. mali' in Selected Psylloidea Species
All species that were at least as abundant as C. picta in the orchards were subjected to a PCR analysis to detect if they carry AP-phytoplasma. In 2015, two out of nine C. picta specimens and one out of 62 C. melanoneura specimens were found infected with 'Ca. P. mali'. In 2016 and 2017, two out of 208 and four out of 77 C. melanoneura specimens, respectively, were tested positive for 'Ca. P. mali'. In 2015 and 2016, 'Ca. P. mali' was not detected in any other species of Psylloidea that were tested (Table 2). Altogether, seven AP-infected individuals of C. melanoneura and two AP-infected individuals of C. picta were collected in the three years of the study. This low number did not allow any statistical evaluation of the effects of daytime and canopy height on sampling AP-infected specimens.

Discussion
A reliable monitoring method is one of the most important prerequisites to estimate the actual density of an insect at a given location. An accurate estimation of these densities is particularly important if these insects transmit plant pathogens like phytoplasmas. The aim of this study was to evaluate if monitoring via the beating tray method (and how it is commonly performed) is a reliable approach for the estimation of the AP phytoplasma transmitting psyllids C. picta and C. melanoneura. In case of AP vectors, this sampling method is usually performed at a canopy height between 1 and 1.5 m and at arbitrary times of the day. However, it has been unclear if AP transmitting psyllids are more abundant in higher parts of the canopy and if more individuals can be collected in the morning when the insects are supposed to be less active. A bias due to a specific distribution of psyllids on trees and due to differences in their activity depending on daytime or temperature could affect the overall density estimation of the vectors. To address these principal questions, C. melanoneura and C. picta densities were monitored via beating tray sampling at two different daytimes and at two different canopy heights. A regression analysis was performed to analyze the correlation between temperature and the probability to catch more C. melanoneura. Based on this regression, a temperature threshold was determined. Two sampling time slots were chosen for the monitoring; a time slot from 7 until 9 a.m. when temperatures are generally lower and a time slot from 2 until 4 p.m. with higher temperatures. Further, two parts on the apple trees were distinguished-the lower and the upper canopy. Samples were taken at 1 m above the ground, where the lowest branches start to grow, and at 2.5 m, which represents the tops of the trees in the orchard under study. During the three-year survey, C. picta densities were too low to perform any statistical analysis. Therefore, we focused on the other AP vector, C. melanoneura. The following questions were addressed: (i) Does the daytime have any effect on the beating tray sampling of C. melanoneura? In 2015, the number of emigrant specimens of C. melanoneura collected was significantly higher in the morning than in the afternoon. However, in the following two years, there was no significant difference and only a slight trend of more specimens in the morning was observed (Figure 2). In 2015, the mean temperatures from April to May/June were 2.5 • C to 4 • C higher in the morning and 3.4 • C to 3.5 • C higher in the afternoon as compared with 2016 and 2017 ( Figure 1 and Table A1). Psyllids are considered ectotherms and thus sensitive to temperature fluctuations during the day and throughout the year [30]. Typically, ectotherms exhibit low activity at cold temperatures. Activity rises to an optimum but drops if temperatures increase further [39,40]. Therefore, it is generally recommended to perform beating in the morning, when temperatures are low and insects are inactive [28]. The higher temperatures in the afternoon in 2015 might have led to a higher jumping, flight and reaction activity of C. melanoneura and a reduced number of catches compared to the morning. In the relatively colder following years, the psyllids might have been generally less active in the afternoon due to lower temperatures and the difference between morning and afternoon catches was therefore less pronounced. The results indicate that C. melanoneura reacts to higher temperatures with an increased flight or jumping activity, which is reflected by less beating tray catches. This is consistent with the determined temperature thresholds of 8.7 • C in the morning or 17.6 • C in the afternoon, respectively (Figure 3). Since the temperatures in the morning and afternoon correlated positively, the threshold for the morning is an indicative value for the temperatures in the afternoon ( Figure A1). The temperature threshold of 17.6 • C in the afternoon actually denotes that more specimens are likely to be collected in the morning (Figure 3). This implies that the flight activity of C. melanoneura at a temperature above 17.6 • C is increased. This temperature threshold was mainly reached from April onwards, when the emigrants started to appear. Indeed, the effect of catching more C. melanoneura individuals in the morning was only documented for emigrants ( Figure 2). However, differences between catches in the morning and the afternoon were statistically significant only if temperatures were above 12 • C (in the morning) and 21.3 • C (in the afternoon) ( Figure 3 and Table A1). Temperatures early in the season were below the threshold of 17.6 • C and therefore the general activity of remigrants is expected to be quite low Plants 2020, 9, 1168 9 of 15 throughout the whole day. This explains why no significant differences between the numbers of remigrants sampled in the morning and in the afternoon occurred.
(ii) Is there any difference in the distribution of C. melanoneura specimens at different canopy heights of apple trees? For some Psylloidea species, such as C. pyri, C. pyricola and Agonoscena targionii, it was shown that their adults prefer to dwell in the upper canopy [29,33,34]. The results of our study indicate that C. melanoneura also shows a non-random distribution on trees. For the remigrants, no difference was observed, but emigrants were collected in higher numbers from the lower canopy ( Figure 4). To cross-evaluate the results from the beating tray sampling, a second method (wash down) was applied to analyze the distribution of the remigrant population, i.e., by quantifying their offspring. The mobility of psyllid nymphs is limited [41] since they lack jumping and flying abilities. Sampling of psyllid nymphs is very laborious but less prone to errors with regards to potentially skewed results caused by an escape of the individuals during the sampling procedure. The distribution pattern of the nymphs on apple trees strongly resembled the adults of the parental remigrant generation in the respective year (Figures 4 and 5). This shows that the data acquired with the beating tray method are not strongly influenced by an escape-driven translocation of adult C. melanoneura remigrants during the sampling. The distribution patterns of the nymphs and the respective adult emigrants were not related. C. melanoneura emigrants might prefer to dwell in the lower parts of the canopy to avoid excessive sunlight and temperatures on the tops of the trees. This is in line with the findings of Rygg, who showed that trap catches of the carrot psyllid Trioza apicalis correlate negatively with light intensity and temperature [38]. The light intensity within the canopy ramps with canopy height from ca. 5-40% of full sun at the bottom (depending on the orchard training system) to 100% at the top of the canopy [42] and temperature is reduced approximately by 2 • C with 30-60% of shading in apple orchards [43]. Due to higher light intensity, C. melanoneura in the upper canopy is also surrounded by a warmer microclimate than in the lower parts of the tree. The higher number of specimens collected from presumably colder lower parts of the trees might thus be caused by a reduced activity of the insects, as described in the previous paragraph. Further, the light intensity at the bottom of the canopy decreases by around 15 to 30% (depending on the orchard training system) after petal fall [42]. Petal fall is followed by leaf growth that leads to shading and temperature reduction in the lower parts of the tree [43]. This might be a further reason why remigrants-which are present before petal fall [11]-are not as affected by shading effects as emigrants. Additionally, the weather may influence the C. melanoneura distribution on the trees. Horton et al. suggested that weather events displaced C. pyricola from the trees and that dislodged psyllids were able to recolonize the tree [35]. It cannot be excluded that rain or wind also affected the psyllid distribution in our study.
The density of C. picta in the orchard was low throughout the whole sampling period, in total only 13 specimens of C. picta were collected. This is in line with generally low densities of C. picta recently reported for South Tyrol [11]. Although the population densities of C. picta in the orchards are usually low also in other parts of Europe (e.g., [27]), this species is considered to be the main vector of apple proliferation wherever it is present [14]. Until now, it was unclear if the applied monitoring strategies are adequate to reveal the actual densities. The results of our study indicate that only few specimens of C. picta are present even in higher parts of the apple tree canopy, which might have been neglected in previous studies and monitoring.
The infection rate with 'Ca. P. mali' varied between 1.5% and 5.2% for C. melanoneura and was 22.2% for C. picta (Table 2). These results are similar to reported infection rates from South Tyrol and fit into the range reported also from other European countries [11,14]. In contrast to other studies [22][23][24], 'Ca. P. mali' was not detected in any other species of Psylloidea that were abundantly present in the orchard, further confirming that C. melanoneura and C. picta are the most relevant vectors of the apple proliferation phytoplasma in South Tyrol.
So far, no treatment against the apple proliferation phytoplasma has been available which could be applied in orchards. Therefore, the spread of 'Ca. P. mali' can be controlled only by uprooting of the infected trees and by phytosanitary measures against the insect vectors. There is a tendency that more emigrants of C. melanoneura can be sampled in the morning than in the afternoon and it should be taken into account that, if 17.6 • C is exceeded, the risk of underestimating the C. melanoneura population density increases if sampling is only performed in the afternoon. It is thus recommended that emigrant sampling is preferentially performed in the morning. For catching remigrants, beating tray sampling can be performed in the morning or in the afternoon. Sampling from the higher canopy of apple trees does not lead to increased numbers of psyllid catches, underlining that the currently used way of beating from the lower parts of trees is sufficient for the determination of C. melanoneura. In conclusion, the results of our study underline that the beating tray method is suitable for estimating densities of C. melanoneura. Nevertheless, above a certain temperature threshold, C. melanoneura density prediction might be skewed.

Study Site
The study was conducted in a commercial apple orchard located in the north-east of Merano (South Tyrol, Northern Italy, 700 m a.s.l., 46 • 64'26" N, 11 • 19'38" E) with approximately 1900 apple trees (mainly 'Golden Delicious'). The trees were planted between 2003 and 2009 and were, on average, 3 m tall. The orchard was treated twice a year with the agent Azadirachtin against aphids, which was first applied during blossom (middle of April) and then 2-4 weeks later (beginning of May). Based on visual inspections of specific symptoms carried out in autumn, the 'Ca. P. mali' infection rate of the apple trees in the orchard was determined as 1.6-2.4%. During sampling days, temperature was measured 1 m above ground in the shadow at 7 a.m., 9 a.m., 2 p.m. and 4 p.m.

Insect Sampling
Beating tray sampling of adults of Psylloidea was carried out from February to July in 2015, 2016 and 2017 in a bi-weekly routine. One branch per apple tree was struck four times using a padded stick, while a rectangular beating tray was held beneath the branch to collect the dislodged psyllids [29]. The sampling was carried out at two daytimes: in the morning (7-9 a.m.) and in the afternoon (2-4 p.m.), as well as at two different canopy heights: in the lower canopy (at 1 m above the ground) and in the upper canopy (at 2.5 m). Every two calendar weeks 50 trees (2015) as well as 40 trees (2016, 2017) were randomly selected and sampled for each sampling daytime and canopy height. This procedure was repeated four times in 2015 and five times in 2016, 2017. A total of 400 trees were sampled per routine.
Wash down sampling of nymphs of Psylloidea was carried out from March to July in 2016 in a bi-weekly routine. Ten shoots (ca. 10 cm long) per apple tree were taken at two different canopy heights: the lower canopy (at 1 m above the ground) and in the upper canopy (at 2.5 m) from 20 randomly selected trees for each two calendar weeks. This procedure was repeated five times and a total of 200 trees were sampled per routine. A plastic bag was carefully put over the shoots and those were cut. The plastic bags were closed tightly. Shoots were soaked for twelve hours in soapsuds at 4 • C in a closed vessel. Afterwards, the shoots were swayed for three minutes in the soapsuds. The shoots and the plastic bag were rinsed with water, which was collected and introduced to the soapsuds [44]. The soapsuds were filtrated (40 µm mesh size), so that nymphs were concentrated on the filter. The filter content was scanned for nymphs using a binocular.

Material Identification
Identification of all adult specimens of Psylloidea was performed based on morphology using identification keys [3,45,46]. The nomenclature and classification in the paper follows Ouvrard [4]. Specimens were stored in 75% ethanol at −20 • C. Specimens that could not be undoubtedly identified by morphology, have been additionally determined with the restriction fragment length polymorphism (RFLP) method [47]. Briefly, PCR was performed in a final volume of 20 µL containing 1× Colorless The digested amplicon was visualized on a 2% Metaphor™ agarose gel and the characteristic digestion pattern was used for Cacopsylla species discrimination [47].
All nymphs were counted and fifth instar nymphs were identified based on morphological characteristics [3].

Detection of 'Ca. P. mali' in Psyllids
For the detection of 'Ca. P. mali' (and to perform RFLP, see above), genomic DNA was extracted from psyllid specimens using the DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany) [48].

Statistical Analysis
To compare the numbers of C. melanoneura specimens between the morning vs. afternoon sampling (all counts on a tree), a Mann-Whitney U test was performed. In addition, total counts per year of C. melanoneura remigrants as well as C. melanoneura emigrants at the morning vs. afternoon were compared using a Chi-square test. To compare the numbers of adult C. melanoneura specimens and nymphs collected at the lower vs. upper canopy (independently of the sampling time of day), the same tests as previously explained were applied. For C. picta, no statistical analysis was performed due to its low number of specimens and low frequency in the samples. For each two calendar weeks, the percentage of specimens caught in the morning and afternoon was calculated (catch probability). Based on the catch probability combined with the corresponding air temperatures in the morning and afternoon, a polynomial regression (2nd degree) was computed. The confidence intervals were defined at a level of 0.95. A threshold was set at a catch probability of 50% and with the lower confidence limit (morning) and the upper confidence limit (afternoon). The mean temperatures in the morning were correlated with the mean temperatures in the afternoon and a linear regression was computed. The confidence intervals were defined at a level of 0.95. No statistical analysis was performed to analyze the effect of sampling daytime and canopy height on the number of AP-infected C. melanoneura and C. picta specimens, due to the low number of AP-infected specimens. All analyses were carried out in the R statistical software (v. 3.1.3).

Funding:
The work was performed as part of the project APPLClust and APPLIII within the framework agreement in the field of invasive species in fruit growing and major pathologies (PROT. VZL_BZ 09.05.2018 0002552), which was co-funded by the Autonomous Province of Bozen/Bolzano, Italy and the South Tyrolean Apple Consortium. The authors thank the Department of Innovation, Research and Universityies of the Autonomous Province of Bozen/Bolzano for covering the Open Access publication costs.

Acknowledgments:
We would like to thank Stefanie Fischnaller for a critical reading of the manuscript, Thomas Letschka for stimulating discussions and scientific input and to Vicky Oberkofler for helping to wash down the nymphs from the shoots.

Conflicts of Interest:
The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.
Appendix A Table A1. Minimum, maximum and mean temperatures at two daytimes of sampling (morning: 7-9 a.m.; afternoon: 2-4 p.m.), in the studied apple orchard per each two calendar weeks from 2015 until 2017.  Figure A1. Correlation between mean temperature in the morning (7-9 am) and mean temperature in the afternoon (2-4 pm) in the studied apple orchard in 2015-2017.