Zinc-lysine Supplementation Mitigates Oxidative Stress in Rapeseed (Brassica napus L.) by Preventing Phytotoxicity of Chromium, When Irrigated with Tannery Wastewater
Abstract
:1. Introduction
2. Materials and Methods
2.1. Collection and Analysis of Wastewater and Soil
2.2. Pot Experiment
2.3. Plant Harvesting
2.4. Analysis Items and Methods
2.5. Statistical Analysis
3. Results
3.1. Effect of Foliar Application of Zn-lys on Plant Growth and Biomass under Different Levels of Tannery Wastewater
3.2. Effect of Foliar Application of Zn-lys on Photosynthetic Pigments and Gas Exchange Attributes under Different Levels of Tannery Wastewater
3.3. Effect of Foliar Application of Zn-lys on Oxidative Stress and Antioxidant Response under Different Levels of Tannery Wastewater
3.4. Effect of Foliar Application of Zn-lys on Uptake and Accumulation of Cr and Zn under Different Levels of Tannery Wastewater
3.5. Correlation between Different Parameters Studied in This Experiment
3.6. Principal Component Analysis
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Texture | Clay Loam |
---|---|
Silt | 12.9 |
Sand | 63.4 |
Clay | 22.3 |
pH (H2O mixture) | 7.1 |
Electrical conductivity (dS m−1) | 3.83 |
Cation exchange capacity (cmol kg−1) 4.9 | 4.89 |
Soluble CO3−2 (mmol L−1) | 0.87 |
Soluble HCO3 (mmol L−1) | 3.78 |
Soluble Cl− (mmol L−1) | 6.31 |
Soluble Ca2+ + Mg2+ (mmol L−1) | 15.89 |
Organic matter (%) | 0.49 |
Ni (mg kg−1) | 0.21 |
Cu (mg kg−1) | 0.35 |
Zn (mg kg−1) | 0.84 |
Cr (mg kg−1) | 0.24 |
Parameters | Values | Permissible Limits * |
---|---|---|
EC (dS m−1) | 1.41 | <1.5 |
Sodium absorption rate (mmol L−1)1/2 | 4.02 | <7.5 |
Residual sodium carbonate (mmol c L−1) | 2.24 | <2.0 |
Ni (mg L−1) | 0.09 | 0.20 |
Cd (mg L−1) | 0.04 | 0.01 |
Pb (mg L−1) | 1.24 | 5.0 |
Co (mg L−1) | 0.02 | 0.05 |
Cr (mg L−1) | 4.03 | 0.10 |
Zn (mg L−1) | 1.95 | 2.00 |
Treatments | Plant Height (cm) | Number of Leaves | Leaf Area (cm2) | Root Length (cm) | Root Fresh Weight (mg) | Root Dry Weight (mg) | Leaf Fresh Weight (mg) | Leaf Dry Weight (mg) |
---|---|---|---|---|---|---|---|---|
Ck | 31 ± 0.6ab | 11 ± 1ab | 110 ± 7ab | 13 ± 0.4cd | 4025 ± 55b | 837 ± 36b | 19,336 ± 740b | 2457 ± 50b |
T1 | 34 ± 1.0a | 13 ± 1a | 126 ± 5a | 17 ± 1a | 5038 ± 70a | 999 ± 46a | 23,675 ± 1200a | 2658 ± 64a |
T2 | 22 ± 1.6de | 6 ± 0.6bc | 72 ± 5d | 12 ± 0.6de | 1661 ± 57d | 334 ± 13d | 11,991 ± 467d | 1573 ± 56d |
T3 | 29 ± 1.2bc | 8 ± 0.6b | 91 ± 4c | 15 ± 0.7ab | 2191 ± 64c | 470 ± 18c | 15,599 ± 561c | 1804 ± 50c |
T4 | 17 ± 0.6ef | 6 ± 1c | 38 ± 3f | 10 ± 0.3e | 798 ± 37f | 198 ± 12e | 5005 ± 100f | 729 ± 30f |
T5 | 24 ± 1.7cd | 8 ± 0.6b | 53 ± 3e | 14 ± 0.8bc | 1179 ± 53e | 292 ± 19d | 6872 ± 170e | 909 ± 30e |
T6 | 10 ± 0.6g | 5 ± 0.6c | 17 ± 1g | 8 ± 0.5f | 414 ± 13g | 88 ± 6f | 3142 ± 70g | 433 ± 24g |
T7 | 11 ± 2.8fg | 6 ± 0.6bc | 19 ± 3g | 10 ± 0.6e | 505 ± 19g | 103 ± 10f | 3665 ± 75fg | 507 ± 28g |
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Zaheer, I.E.; Ali, S.; Saleem, M.H.; Arslan Ashraf, M.; Ali, Q.; Abbas, Z.; Rizwan, M.; El-Sheikh, M.A.; Alyemeni, M.N.; Wijaya, L. Zinc-lysine Supplementation Mitigates Oxidative Stress in Rapeseed (Brassica napus L.) by Preventing Phytotoxicity of Chromium, When Irrigated with Tannery Wastewater. Plants 2020, 9, 1145. https://doi.org/10.3390/plants9091145
Zaheer IE, Ali S, Saleem MH, Arslan Ashraf M, Ali Q, Abbas Z, Rizwan M, El-Sheikh MA, Alyemeni MN, Wijaya L. Zinc-lysine Supplementation Mitigates Oxidative Stress in Rapeseed (Brassica napus L.) by Preventing Phytotoxicity of Chromium, When Irrigated with Tannery Wastewater. Plants. 2020; 9(9):1145. https://doi.org/10.3390/plants9091145
Chicago/Turabian StyleZaheer, Ihsan Elahi, Shafaqat Ali, Muhammad Hamzah Saleem, Muhammad Arslan Ashraf, Qurban Ali, Zohaib Abbas, Muhammad Rizwan, Mohamed A. El-Sheikh, Mohammed Nasser Alyemeni, and Leonard Wijaya. 2020. "Zinc-lysine Supplementation Mitigates Oxidative Stress in Rapeseed (Brassica napus L.) by Preventing Phytotoxicity of Chromium, When Irrigated with Tannery Wastewater" Plants 9, no. 9: 1145. https://doi.org/10.3390/plants9091145