Polyphenolic Composition and Anti-Melanoma Activity of White Forsythia (Abeliophyllum distichum Nakai) Organ Extracts

Abeliophyllum distichum Nakai, commonly called white forsythia, is a monotypic genus endemic to Korea. Although A. distichum is mainly used as an ornamental plant because of its horticultural value, recent studies have demonstrated its bioactivities, including antioxidant and anti-inflammatory activities, prompting us to investigate the potential anticancer effect of A. distichum organ extracts (leaves, fruit, and branches) against human melanoma SK-MEL-2 cells. The methanol extract of A. distichum leaves (AL) exhibited dose- and time-dependent cytotoxicities against SK-MEL-2 cells but not against HDFa human dermal fibroblasts. Based on high-performance liquid chromatography analysis, we identified 18 polyphenolic compounds from A. distichum organ extracts and suggest that differences in anticancer activity between organ extracts should be caused by different compositions of polyphenolic compounds. Additionally, the Annexin V/propidium iodide staining assay and analysis of caspase activity and expression indicated that AL induced cell death, including early and late apoptosis, as well as necrosis, by inducing the extrinsic pathway. Furthermore, we analyzed the differentially expressed genes between mock- and AL-treated cells using RNA-seq technology, suggesting that the anti-melanoma action of AL is mediated by down-regulation of the phosphoinositide 3-kinase (PI3K)/Akt signaling pathway. Taken together, these results shed light on the potential use of A. distichum as a green resource with potent anti-melanoma activity.


Introduction
Malignant melanoma is the deadliest skin cancer type that originates from pigment-producing cells known as melanocytes [1]. It is caused by multiple and progressive DNA damage related to proto-oncogene activation, down-regulation of tumor suppressor genes, and structural changes of the chromosomes [2]. The etiology of all cancers depends on the interplay between genetic and environmental factors [3], and approximately 60-70% of malignant melanomas are presumed to be caused by ultraviolet (UV) radiation exposure [4]. Since malignant melanomas have been treated by surgical removal, cryosurgery, radiation therapy, or chemotherapy, there has been a growing interest in the use of complementary and alternative medicines because of the disadvantages mediated by conventional cancer chemotherapies and the health benefits of phytochemicals [5]. Different mechanisms and pathways are regulated by the anti-melanoma activity of phytochemicals, such as increased apoptosis, caspase activity, and expression of p53 and Bcl-2-associated X protein Plants 2020, 9, 757 2 of 13 (bax), and the inhibition of angiogenesis and tumor-promoting proteins [5]. The functional diversity of phytochemicals should provide unique and renewable resources to discover and develop potential new antitumor agents, suggesting that the investigation of biological and pharmaceutical properties of medicinal plants and their natural products is an important issue in the development of complementary or adjunctive therapies.
Abeliophyllum distichum Nakai is a deciduous flowering shrub, a monotypic genus with a single species of deciduous shrub in the olive family [6]. Although A. distichum has been used as a horticultural crop because of its ornamental value, the pharmaceutical properties of A. distichum extracts have been recently revealed, such as the anti-inflammatory effect [7], antioxidant activity [6], DNA damage inhibition [8], whitening property [9], anti-diabetic effect by inhibiting aldose reductase [10], antihypertensive activity [11], and anti-proliferative activity against human colorectal cancer cells [12]. Additionally, phytochemical investigations of A. distichum have revealed the presence of multiple active ingredients, such as acteoside, eutigoside B, isoacteoside, rutin, cornoside, hirsutrin, chlorogenic acid, caffeic acid, gentisic acid, ferulic acid, and quercetin [6,10,11], indicating the potential of A. distichum to be developed as a phytomedicine and a source to develop anti-melanoma agents, although no systematic study exists regarding the anti-melanoma action of A. distichum.
Therefore, in this study, the methanol extracts of A. distichum fruit, branches, and leaves were analyzed to assess the anti-melanoma effect of A. distichum, as well as to identify and quantify polyphenolic compounds, using high-performance liquid chromatography (HPLC). Additionally, to investigate the possible mechanism of anti-melanoma action of A. distichum extract, we performed pathway enrichment analysis of the differentially expressed genes (DEGs) in response to A. distichum extract.

Effects of A. distichum Organ Extracts on the Viability of Human Melanoma SK-MEL-2 Cells
The effect of the methanol extracts of leaves, branches, and fruit on the growth of human SK-MEL-2 melanoma cells was investigated using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. As shown in Figure 1A, incubation with 50 µg/ml of A. distichum leaves (AL) (48.091 ± 12.741%) strongly inhibited the proliferation of SK-MEL-2 cells compared with A. distichum branches (AB) (84.229 ± 7.335%) and A. distichum fruits (AF) (86.949 ± 6.287%), although 200 µg/ml of all extracts showed similar cytotoxic activity. Therefore, AL was selected for further experiments. AL exhibited a dose-and time-dependent inhibitory effect on the viability of SK-MEL-2 cells ( Figure 1B). Although chemotherapeutic agents, including irinotecan, doxorubicin, oxaliplatin, and cyclophosphamide, have shown promising results alone or in combination with other cancer therapies, their unexpected activities against normal cells have led to harmful side effects [13]. Therefore, identifying and developing tumor-specific agents remains an outstanding challenge in cancer therapy. To investigate the effect of AL on the viability of normal cells and other melanoma cells, murine melanoma (B16F10) cells and adult human dermal fibroblasts (HDFa) were treated with different concentrations (50 µg/ml, 100 µg/ml and 200 µg/ml) of AL for 48 h. As shown in Figure 1C, AL showed cytotoxic effects on B16F10 cells at concentrations of 50-200 µg/ml, but it was not cytotoxic against HDFa, even at the maximum tested concentrations. These findings indicate that AL can be a potent source of anticancer agents because of its selective cytotoxicity against melanoma cells and lack of toxicity in non-cancerous cells.

Composition of Polyphenolic Compounds in A. distichum Organ Extracts
Polyphenolic compounds, including flavonoids and tannins, are beneficial plant compounds that offer various health benefits and are important anticancer agents [14]. Additionally, they are potential active compounds in A. distichum extract. To identify the active polyphenolic compounds that potentially exhibit cytotoxicity against melanoma cells, phenolic and flavonoid compounds were identified and quantified in A. distichum organ extracts using HPLC. Eighteen polyphenolic compounds, including chlorogenic acid, gallic acid, and luteolin, were identified in A. distichum organ extract (Table 1). AL (78.054 ± 0.307 mg/g of extract) and AF (36.012 ± 0.392 mg/g of extract) contain gallic acid (Table 1), which induces apoptosis in cancer cells [15]. Additionally, the highest amount of taxifolin, which exhibits promising pharmacological activities, including the management of tumors and liver disorders [16], was detected in AL (16.977 ± 0.167 mg/g of extract). Although AB exhibited a low level of cytotoxic activity compared with other extracts, various polyphenolic compounds, including kaempferol, rhamnetin, tangeretin, vanillic acid, and ferulic acid, have been identified. Taken together, the results show that the anti-melanoma activity of A. distichum organ extract should be mediated by these active polyphenolic compounds together with phytochemicals, which were not identified in this study. Additionally, differences in the anti-melanoma activity of organ extracts might be mediated by the different compositions of polyphenolic compounds.

Composition of Polyphenolic Compounds in A. distichum Organ Extracts
Polyphenolic compounds, including flavonoids and tannins, are beneficial plant compounds that offer various health benefits and are important anticancer agents [14]. Additionally, they are potential active compounds in A. distichum extract. To identify the active polyphenolic compounds that potentially exhibit cytotoxicity against melanoma cells, phenolic and flavonoid compounds were identified and quantified in A. distichum organ extracts using HPLC. Eighteen polyphenolic compounds, including chlorogenic acid, gallic acid, and luteolin, were identified in A. distichum organ extract (Table 1). AL (78.054 ± 0.307 mg/g of extract) and AF (36.012 ± 0.392 mg/g of extract) contain gallic acid (Table 1), which induces apoptosis in cancer cells [15]. Additionally, the highest amount of taxifolin, which exhibits promising pharmacological activities, including the management of tumors and liver disorders [16], was detected in AL (16.977 ± 0.167 mg/g of extract). Although AB exhibited a low level of cytotoxic activity compared with other extracts, various polyphenolic compounds, including kaempferol, rhamnetin, tangeretin, vanillic acid, and ferulic acid, have been identified. Taken together, the results show that the anti-melanoma activity of A. distichum organ extract should be mediated by these active polyphenolic compounds together with phytochemicals, which were not identified in this study. Additionally, differences in the anti-melanoma activity of organ extracts might be mediated by the different compositions of polyphenolic compounds.

AL Induces Caspase 8-dependent Apoptosis in SK-MEL-2 Cells
Apoptosis and necrosis are highly conserved cell death mechanisms involved in the elimination of cancer cells by anti-cancer agents [17]. Distinguishing specific cell death subtypes is highly important to develop antineoplastic or other therapeutic drugs. To investigate the cell death type induced by AL, early-stage apoptotic cells and dead cells (late-stage apoptotic or necrotic cells) were discriminated using Annexin V/propidium iodide (PI) staining assay combined with fluorescence plate reader measurements. As shown in Figure 2A, early-stage apoptotic cells and dead cells were significantly increased in SK-MEL-2 cells after AL treatment in a dose-dependent manner, suggesting that AL-induced cell death is comparable between early apoptotic and dead cells (late-stage apoptotic or necrotic cells) in SK-MEL-2 cells.
Reactive oxygen species (ROS), generated through various extracellular and intracellular actions, function as very important intracellular messengers involved in the growth, differentiation, progression, metabolism, and death of cells [18]. In cancer cells, low doses of ROS promote cell proliferation in various cancer cell types, although excessive levels of ROS activate several signaling pathways involved in cell apoptosis [19]. Therefore, ROS generation by phytochemicals has been closely associated with the induction of apoptosis in cancers [20]. To investigate whether AL-induced cell death is mediated by ROS accumulation, the intracellular ROS level in AL-treated cells was measured using the fluorogenic probe DCFH-DA. As shown in Figure 2B, AL treatment did not result in the enhancement of intracellular ROS production, indicating that AL is a source of anticancer compounds that will not cause oxidative damage to normal cells.
Caspase-3, an intracellular cysteine protease, is the main executioner of apoptosis [21]. When SK-MEL-2 cells were treated with AL, caspase-3 activity was increased in a dose-dependent manner ( Figure 3A), indicating that AL suppressed the viability of SK-MEL-2 cells and led to programmed cell death through the modulation of caspase-3 activity. Caspase-3 activation is induced by caspase-8 and caspase-9, which are involved in the extrinsic and intrinsic apoptotic pathways, respectively [22]. To determine the underlying mechanism of AL-induced cell death, we analyzed the gene expression level of caspase-8 and caspase-9 in AL-treated SK-MEL-2 cells. An increased level of caspase-8 and caspase-3 expression was observed after AL treatment, whereas caspase-9 expression was decreased ( Figure 3B). Additionally, down-regulation of the Bax/Bcl-2 ratio, which regulates activation of the intrinsic apoptotic pathway [23], was observed after AL treatment, indicating that the extrinsic pathway triggers apoptosis in response to AL.
Plants 2020, 9, 757 5 of 13 [22]. To determine the underlying mechanism of AL-induced cell death, we analyzed the gene expression level of caspase-8 and caspase-9 in AL-treated SK-MEL-2 cells. An increased level of caspase-8 and caspase-3 expression was observed after AL treatment, whereas caspase-9 expression was decreased ( Figure 3B). Additionally, down-regulation of the Bax/Bcl-2 ratio, which regulates activation of the intrinsic apoptotic pathway [23], was observed after AL treatment, indicating that the extrinsic pathway triggers apoptosis in response to AL.

AL Induces Cell Death via the MEK-ERK Independent Pathway
The mitogen-activated protein kinase (MAPK) pathway is essential in regulating cellular processes, including proliferation, differentiation, development, survival, and, under some conditions, also apoptosis [24]. Therefore, the involvement of MAPKs has been highlighted recently in various cell death-inducing models. To investigate the involvement of the MAPK pathway in AL- Plants 2020, 9, x 5 of 13 [22]. To determine the underlying mechanism of AL-induced cell death, we analyzed the gene expression level of caspase-8 and caspase-9 in AL-treated SK-MEL-2 cells. An increased level of caspase-8 and caspase-3 expression was observed after AL treatment, whereas caspase-9 expression was decreased ( Figure 3B). Additionally, down-regulation of the Bax/Bcl-2 ratio, which regulates activation of the intrinsic apoptotic pathway [23], was observed after AL treatment, indicating that the extrinsic pathway triggers apoptosis in response to AL.

AL Induces Cell Death via the MEK-ERK Independent Pathway
The mitogen-activated protein kinase (MAPK) pathway is essential in regulating cellular processes, including proliferation, differentiation, development, survival, and, under some conditions, also apoptosis [24]. Therefore, the involvement of MAPKs has been highlighted recently in various cell death-inducing models. To investigate the involvement of the MAPK pathway in AL-

AL Induces Cell Death via the MEK-ERK Independent Pathway
The mitogen-activated protein kinase (MAPK) pathway is essential in regulating cellular processes, including proliferation, differentiation, development, survival, and, under some conditions, also apoptosis [24]. Therefore, the involvement of MAPKs has been highlighted recently in various cell death-inducing models. To investigate the involvement of the MAPK pathway in AL-induced cell death, we analyzed the phosphorylation (activation) status of major MAPKs, MEK1/2 and ERK1/2. As shown in Figure 4A, dynamic activation of MEK1/2 and ERK 1/2 was observed after AL treatment. Although several phytochemicals that can inhibit the MEK-ERK pathway have been suggested as potential cancer drugs [25], MEK-ERK-dependent multiple caspase activation is essential for mouse fibroblast and glioma cell death [26,27]. Therefore, we presumed that activation of the MEK-ERK pathway is involved in AL-induced cell death. To test this hypothesis, SK-MEL-2 cells were co-treated with AL (100 µg/ml and 200 µg/ml) and the MEK1/2 selective inhibitor PD98059 for 48 h, and cell death was determined using MTT assay. PD98059 did not inhibit AL-induced cell death ( Figure 4B), suggesting that activation of the MEK-ERK pathway is not essential for the anticancer activity of AL against SK-MEL-2 cells.
suggested as potential cancer drugs [25], MEK-ERK-dependent multiple caspase activation is essential for mouse fibroblast and glioma cell death [26,27]. Therefore, we presumed that activation of the MEK-ERK pathway is involved in AL-induced cell death. To test this hypothesis, SK-MEL-2 cells were co-treated with AL (100 μg/ml and 200 μg/ml) and the MEK1/2 selective inhibitor PD98059 for 48 h, and cell death was determined using MTT assay. PD98059 did not inhibit AL-induced cell death ( Figure 4B), suggesting that activation of the MEK-ERK pathway is not essential for the anticancer activity of AL against SK-MEL-2 cells.

AL Down-Regulates the Oncogenic Phosphoinositide 3-kinase (PI3K)/Akt Pathway in SK-MEL-2 Cells
DEG analysis using RNA-seq is a powerful method that can explain the biological changes during the development of a new drug and describe the molecular mechanism of new agents according to the gene expression pattern [28]. To obtain a global overview of the molecular mechanisms involved in AL-induced cell death, total RNA was extracted from mock-or AL-treated cells and was used to generate transcriptome libraries on an Illumina HiSeq™ 2500 sequencing platform. After removing low-quality reads, 67 to 78 million clear reads (9.8 to 11.5 Gb) from each sample were acquired for DEGs analysis. By employing an RPKM (reads per kilobase per million mapped reads) cut-off of 0.05 to identify DEGs, 710 DEGs (182 up-regulated and 528 down-regulated) were detected comparing the mock-and AL-treated libraries (Table S1). To understand the universal response of SK-MEL-2 cells to 200 μg/ml of AL, all screened DEGs were subjected to gene ontology (GO) enrichment analysis, resulting in 54 functional groups classified into three categories: "cellular component", "molecular function", and "biological process" ( Figure S1). The category "biological process", comprising 27 functional groups, exhibited the highest number of annotations alongside "cellular process", followed by "biological regulation", "metabolic process", and "regulation of biological process". Genes within the same pathway usually cooperate to exercise their biological function [29], suggesting that pathway enrichment is a useful strategy to help shed light on the biological mechanism. Pathway enrichment analysis of the identified DEGs revealed that DEGs were mostly enriched in the PI3K/Akt signaling pathway and Rap1 signaling pathway in SK-MEL-2 cells ( Figure S2A). Among DEGs, nine genes, PDGFC, IGF1, KDR, IGF1R, PIK3R1, ITGB3, PDGFD, Akt3,

AL Down-Regulates the Oncogenic Phosphoinositide 3-kinase (PI3K)/Akt Pathway in SK-MEL-2 Cells
DEG analysis using RNA-seq is a powerful method that can explain the biological changes during the development of a new drug and describe the molecular mechanism of new agents according to the gene expression pattern [28]. To obtain a global overview of the molecular mechanisms involved in AL-induced cell death, total RNA was extracted from mock-or AL-treated cells and was used to generate transcriptome libraries on an Illumina HiSeq™ 2500 sequencing platform. After removing low-quality reads, 67 to 78 million clear reads (9.8 to 11.5 Gb) from each sample were acquired for DEGs analysis. By employing an RPKM (reads per kilobase per million mapped reads) cut-off of 0.05 to identify DEGs, 710 DEGs (182 up-regulated and 528 down-regulated) were detected comparing the mock-and AL-treated libraries (Table S1). To understand the universal response of SK-MEL-2 cells to 200 µg/ml of AL, all screened DEGs were subjected to gene ontology (GO) enrichment analysis, resulting in 54 functional groups classified into three categories: "cellular component", "molecular function", and "biological process" ( Figure S1). The category "biological process", comprising 27 functional groups, exhibited the highest number of annotations alongside "cellular process", followed by "biological regulation", "metabolic process", and "regulation of biological process". Genes within the same pathway usually cooperate to exercise their biological function [29], suggesting that pathway enrichment is a useful strategy to help shed light on the biological mechanism. Pathway enrichment analysis of the identified DEGs revealed that DEGs were mostly enriched in the PI3K/Akt signaling pathway and Rap1 signaling pathway in SK-MEL-2 cells ( Figure S2A). Among DEGs, nine genes, PDGFC, IGF1, KDR, IGF1R, PIK3R1, ITGB3, PDGFD, Akt3, and LPAR3, were involved in both the PI3K/Akt signaling pathway and Rap1 signaling pathway ( Figure S2B), supporting that PI3K and Akt serve as downstream signaling molecules of Rap1 [30]. To validate the gene expression results from RNA-seq, the Akt 3 expression ( Figure S3A) and protein levels ( Figure S3B) of Akt3 were analyzed using qRT-PCR and western blotting. As expected, Akt3 showed a consistent expression pattern with its profile revealed using RNA-Seq ( Figure S3A). The PI3K/Akt pathway signaling plays a significant role in the prevention of apoptosis by inhibiting caspase-dependent apoptosis [31,32]. In SK-MEL-2 cells, most of the DEGs involved in the PI3K/Akt pathway were down-regulated by AL treatment ( Table 2), suggesting that AL-induced caspase-8 and -3 might be mediated by down-regulation of the PI3K/Akt pathway.

Discussion
Although cancer therapies have been performed throughout history, the efficacy and safety of treatment remain a challenge. Thus, medicinal plants and phytochemicals have been suggested as complementary treatments and primary sources of synthetic drugs. In this study, we have determined the anti-melanoma activity of A. distichum organ extracts and suggest that AL is a potent source of selective anticancer agents (Figure 1). The difference in the anti-melanoma activity found here between different tested extracts should be due to the difference in the composition of phytochemicals, including polyphenolic compounds (Table 1). One possible explanation for the difference in the composition of polyphenolic compounds is the organ-specific expression of genes associated with polyphenolic compounds. The organ-specific expression pattern of anthocyanin biosynthesis-related genes affects the organ-specific accumulation of metabolites in A. distichum [33]. Additionally, other factors, such as catabolism, translocation, and feedback regulation, play important roles in the organ-specific accumulation of metabolites [34].
Caspases are a family of cysteine proteases that are responsible for apoptosis initiation and execution and can be activated via two main apoptotic signaling mechanisms: the intrinsic (mitochondrial) pathway and extrinsic (receptor-mediated) pathway [35]. In AL-treated SK-MEL-2 cells, we observed the enhanced activity of caspase-3 ( Figure 3A), which converges both apoptotic signaling pathways [21]. However, gene expression analysis indicated that AL induces cell death via activation of the extrinsic pathway ( Figure 3B). In human colorectal cancer cells, the ethyl acetate fraction of 80% methanol extract obtained from the plant parts of A. distichum including the flower, leaf, and branch induced transcriptional activation of activating transcription factor 3 (ATF3), resulting in apoptosis induction [12]. Various human cancers, including liver cancer, prostate cancer, colon cancer, and multiple myeloma, exhibit a low level of ATF3 expression [36][37][38][39]. Additionally, the knockdown mutation of ATF3 promotes the development of prostate cancer through activation of PI3K/Akt signaling [40], which is crucial for the growth, survival, and development of prostate cancer cells [41] and suggests that ATF3 is a promising cancer preventive-therapeutic target. Similarly, we observed up-regulation of ATF3 expression (Table S1), but down-regulation of the PI3K/Akt signaling pathway when SK-MEL-2 cells were treated with AL (Table 2). Interestingly, treatment with perifosine, an Akt inhibitor, induces extrinsic caspase-dependent cell death [42]. Additionally, gallic acid, the major compound identified from AL (Table 1), has been shown to inhibit the activation of Akt in OVCAR-3 cancer cells, resulting in the suppression of cell viability [43]. Furthermore, AL contained the highest content of taxifolin (Table 1), which suppressed the PI3K/Akt/mTOR pathway and scar cell carcinoma growth by inducing apoptosis [44]. Taken together, these results indicate that AL-induced cell death is mediated by down-regulation of the PI3K/Akt signaling pathway and activation of caspase 8-dependent apoptosis ( Figure 5). leaf, and branch induced transcriptional activation of activating transcription factor 3 (ATF3), resulting in apoptosis induction [12]. Various human cancers, including liver cancer, prostate cancer, colon cancer, and multiple myeloma, exhibit a low level of ATF3 expression [36][37][38][39]. Additionally, the knockdown mutation of ATF3 promotes the development of prostate cancer through activation of PI3K/Akt signaling [40], which is crucial for the growth, survival, and development of prostate cancer cells [41] and suggests that ATF3 is a promising cancer preventive-therapeutic target. Similarly, we observed up-regulation of ATF3 expression (Table S1), but down-regulation of the PI3K/Akt signaling pathway when SK-MEL-2 cells were treated with AL (Table 2). Interestingly, treatment with perifosine, an Akt inhibitor, induces extrinsic caspase-dependent cell death [42]. Additionally, gallic acid, the major compound identified from AL (Table 1), has been shown to inhibit the activation of Akt in OVCAR-3 cancer cells, resulting in the suppression of cell viability [43]. Furthermore, AL contained the highest content of taxifolin (Table 1), which suppressed the PI3K/Akt/mTOR pathway and scar cell carcinoma growth by inducing apoptosis [44]. Taken together, these results indicate that AL-induced cell death is mediated by down-regulation of the PI3K/Akt signaling pathway and activation of caspase 8-dependent apoptosis ( Figure 5).

Conclusion
In conclusion, the results of the present study indicate the anti-melanoma potential of AL. AL positively or negatively regulates the complex network of signaling pathways involved in cell cytotoxicity, indicating that the activity described here may be caused by a combination of several potential anti-melanoma compounds. In addition, further study is necessary to analyze its effect on cancer animal models.

Conclusions
In conclusion, the results of the present study indicate the anti-melanoma potential of AL. AL positively or negatively regulates the complex network of signaling pathways involved in cell cytotoxicity, indicating that the activity described here may be caused by a combination of several potential anti-melanoma compounds. In addition, further study is necessary to analyze its effect on cancer animal models. for mitogen-activated protein kinase (MEK)1/2, phospho-MEK1/2 (Ser217/221), extracellular signal-regulated kinase (ERK)1/2, and phospho-ERK1/2 (Thr202/Tyr204) were obtained from Cell Signaling Technology (Beverly, Massachusetts, USA). The β-actin antibody was purchased from Santa Cruz Biotechnology, Inc. (Dallas, Texas, USA). The caspase-3 assay kit was purchased from Biovision (Milpitas, California, USA). The Annexin V FITC assay kit was purchased from Cayman Chemical (Ann Arbor, Michigan, USA).

Extraction and Sample Preparation
A. distichum fruit, branches, and leaves were grown and taken from the research forest at Chungbuk National University. The fruit, branches, and leaves of A. distichum were lyophilized and ground into a fine powder using a blender. The ground materials were soaked in methanol for 24 h and subjected to ultrasonication (1 h × 3 times). After filtration, the methanol extracts of the fruit (AF), branches (AB), and leaves (AL) were evaporated using a rotary vacuum evaporator and kept in a refrigerator until use.

Cell Culture
SK-MEL-2 human melanoma cells, B16F10 murine melanoma cells, and HDFa human dermal fibroblasts were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) and antibiotics or in DMEM medium with 10% FBS and antibiotics, at 37 • C in a humidified chamber containing 5% CO 2 .

Cell Viability Assay
Cell viability was analyzed using MTT assay as described by Jin et al. [45]. Cells were plated in 96-well plates at a density of 2 × 10 4 cells/well for SK-MEL-2 cells or 1 × 10 4 cells/well for B16F10 cells or HDFa cells and then were cultured for 24 h. Next, the cells were treated with different concentrations (25 to 400 µg/ml) of each organ extract. After treatment for 48 h (or respective time points), 20 µL of MTT solution (1 mg/ml, diluted with phosphate-buffered saline (PBS)) were added and the cells were incubated in a CO 2 incubator for an additional 4 h. The produced formazan dissolved with DMSO was quantified at 550 nm using an iMARK™ microplate reader (Bio-Rad, Hercules, CA, USA). An extract amount < 50 µg/ml did not show significant cytotoxic activity, whereas > 200 µg/ml of extracts exhibited similar cytotoxic activity compared to 200 µg/ml of extracts. Based on this finding, three different concentrations (50 µg/ml, 100 µg/ml, and 200 µg/ml) of each organ extract were used for further analysis.

Intracellular Reactive Oxygen Species (ROS) Measurement
Intracellular ROS were detected using the fluorogenic probe DCFH-DA as described by Warleta et al. [46] with minor modifications. SK-MEL-2 cells were exposed to different concentrations (50 µg/ml, 100 µg/ml, and 200 µg/ml) of AL for 5 h and then were stained with 20 µM DCFH-DA. After incubation in a CO 2 incubator for 30 min, the cells were washed with PBS, and then DCF fluorescence was measured using a SpectraMAX Gemini EM Fluorescence Microplate Reader (Molecular Devices, San Jose, CA, USA) at the excitation and emission wavelengths of 485 nm and 525 nm, respectively.

Annexin V/propidium Iodide (PI) Staining Assay
Early-stage apoptotic cells and dead cells were analyzed using an Annexin V FITC Assay Kit (Cayman Chemical, Ann Arbor, MI, USA) according to the manufacturer's instructions. Early-stage apoptotic cells stained using Annexin V FITC were detected with excitation and emission wavelengths of 560 nm and 595 nm, respectively, whereas dead cells stained using PI were detected with excitation and emission wavelengths of 485 nm and 535 nm, respectively, using a SpectraMAX Gemini EM Fluorescence Microplate Reader (Molecular Devices, San Jose, CA, USA).

Caspase-3 Activity
Caspase-3 activity was analyzed using a Caspase-3/CPP32 Fluorometric Assay Kit (BioVision, Milpitas, CA, USA) according to the manufacturer's instructions. Fifty micrograms of protein obtained from mock-or AL-treated cells were mixed with each substrate and then incubated at 37 • C for 2 h. Caspase-3 activity was measured using a fluorescence microplate reader equipped with a 400-nm excitation filter and a 505-nm emission filter.

RNA Sequencing and DEG Analysis
Total RNA extracted from mock-or AL-treated cells was quantified using a DeNovix DS-11 (DeNovix Inc, Wilmington, DE, USA). The cDNA library was synthesized and sequenced using an Illumina HiSeq™ 2500 sequencing platform. Adapter sequences, empty reads, low-quality reads (with ambiguous sequence, N), and reads with more than 10% Q < 20 bases (i.e., with a base quality less than 20) were discarded from the raw sequencing data using the FastQC and Trimmomatic tools. Next, the clean reads were mapped to the Homo sapiens genome reference (Homo sapiens genome assembly GRCh38.p13) using an HISAT2 aligner. Transcript levels were calculated using SAMtools, and relative transcript abundances were analyzed using DEseq [47]. Statistically significant DEGs (p value cutoff of 0.05 and adjusting to |log2 (fold change) |≥ 1) were analyzed for gene ontology (GO) enrichment and pathway enrichment using the Blast2GO program and KOBAS v.3.0 Server, respectively. The statistical significance threshold level for all GO enrichment and pathway enrichment was p < 0.05. All reads were deposited in the National Agricultural Biotechnology Information Center (NABIC, http://nabic.rda.go.kr) with accession number NN-6325 and NN-6326.

Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR)
One hundred nanograms of total RNA were reverse transcribed into cDNA using a ReverTra Ace®qPCR RT Master Mix with qDNA Remover (TOYOBO Co., Ltd, Osaka, Japan) according to the manufacturer's instructions. qRT-PCR was performed using SYBR®Green Real-time PCR Master Mix (TOYOBO, Co., Ltd, Osaka, Japan) in a CFX96TM Real-Time System (Bio-Rad, Hercules, CA, USA). The relative expression levels of selected genes were normalized to the expression of the internal reference gene GAPDH. The specific primer pairs are listed in Table S2.

HPLC Analysis
HPLC analysis was performed using a Shimadzu liquid chromatography system (LC-10ADvp) coupled with an ultraviolet-visible detector (SPD-10A; Shimadzu, Japan). Separation was achieved using a Luna®HPLC column (C18(2) 100 Å, 250 × 4.6 mm, 5 µm) at 35 • C with a 0.7-ml/min flow rate. The mobile phases consisted of water containing 0.1% trifluoroacetic acid (mobile phase A) and acetonitrile containing 0.1% trifluoroacetic acid (mobile phase B). The following gradient elution was performed: 10% B in 0-0.01 min, 10-40% B in 0.01−28 min, 40-60% B in 28-39 min, 60-90% B in 39-50 min, and holding at 90% B for 5 min. The concentration was calculated by comparing the peak areas of the samples with the calibration curve of the standards.

Statistical Analysis
All experiments were performed for three independent replicates, and the data were expressed as means ± standard error (SE). The significance of the between-group differences was determined using analysis of Duncan's multiple range test. Values of p < 0.05 were considered significant.