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Article

The First Case of Glyphosate Resistance in Johnsongrass (Sorghum halepense (L.) Pers.) in Europe

1
Agricultural Chemistry and Soil Sciences, University of Córdoba, 14014 Cordoba, Spain
2
Departamento de Ciencias Agroforestales, Escuela Tecnica Superior de Ingenieria, Campus de La Rabida, Universidad de Huelva, Palos de la Frontera, 21819 Huelva, Spain
*
Author to whom correspondence should be addressed.
Plants 2020, 9(3), 313; https://doi.org/10.3390/plants9030313
Received: 29 January 2020 / Revised: 20 February 2020 / Accepted: 26 February 2020 / Published: 3 March 2020
Six Johnsongrass populations suspected of being glyphosate resistant were collected from railways and freeways near Cordoba (SW Spain), where glyphosate is the main weed control tool. The 50% reduction in shoot fresh weight (GR50) values obtained for these six populations ranged from 550.4 to 1169 g ae ha−1, which were 4.2 to 9 times greater than the value obtained for the susceptible population. Glyphosate was equally metabolized to the same extent in both resistant and susceptible populations, with no significant differences in either 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) inhibition or basal activity. No amino acid substitutions were observed in any of the resistant populations. Slight but significant differences in glyphosate penetration were observed among some but not all of the resistant populations and for the times of incubation assayed, although these differences were not considered further. The proposed primary mechanism of resistance in these six glyphosate-resistant Johnsongrass populations is reduced herbicide translocation, because the amount of glyphosate that translocated from treated leaves to shoots and roots in the susceptible population was double that observed in the resistant populations. As glyphosate multiple resistance due to more than one mechanism is not uncommon, this is the first time that glyphosate-resistant Johnsongrass populations have been fully described for all known mechanisms. View Full-Text
Keywords: translocation; metabolism; penetration; EPSPS; non-target site resistance; resistant weeds translocation; metabolism; penetration; EPSPS; non-target site resistance; resistant weeds
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MDPI and ACS Style

Vazquez-Garcia, J.G.; Palma-Bautista, C.; Rojano-Delgado, A.M.; De Prado, R.; Menendez, J. The First Case of Glyphosate Resistance in Johnsongrass (Sorghum halepense (L.) Pers.) in Europe. Plants 2020, 9, 313. https://doi.org/10.3390/plants9030313

AMA Style

Vazquez-Garcia JG, Palma-Bautista C, Rojano-Delgado AM, De Prado R, Menendez J. The First Case of Glyphosate Resistance in Johnsongrass (Sorghum halepense (L.) Pers.) in Europe. Plants. 2020; 9(3):313. https://doi.org/10.3390/plants9030313

Chicago/Turabian Style

Vazquez-Garcia, Jose G., Candelario Palma-Bautista, Antonia M. Rojano-Delgado, Rafael De Prado, and Julio Menendez. 2020. "The First Case of Glyphosate Resistance in Johnsongrass (Sorghum halepense (L.) Pers.) in Europe" Plants 9, no. 3: 313. https://doi.org/10.3390/plants9030313

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