Genetically Modified Heat Shock Protein90s and Polyamine Oxidases in Arabidopsis Reveal Their Interaction under Heat Stress Affecting Polyamine Acetylation, Oxidation and Homeostasis of Reactive Oxygen Species

One Sentence Summary Heat shock proteins90 (HSP90s) induce acetylation of polyamines (PAs) and interact with polyamine oxidases (PAOs) affecting oxidation of PAs and hydrogen peroxide (H2O2) homeostasis in Arabidopsis thaliana. Abstract The chaperones, heat shock proteins (HSPs), stabilize proteins to minimize proteotoxic stress, especially during heat stress (HS) and polyamine (PA) oxidases (PAOs) participate in the modulation of the cellular homeostasis of PAs and reactive oxygen species (ROS). An interesting interaction of HSP90s and PAOs was revealed in Arabidopsis thaliana by using the pLFY:HSP90RNAi line against the four AtHSP90 genes encoding cytosolic proteins, the T-DNA Athsp90-1 and Athsp90-4 insertional mutants, the Atpao3 mutant and pharmacological inhibitors of HSP90s and PAOs. Silencing of all cytosolic HSP90 genes resulted in several-fold higher levels of soluble spermidine (S-Spd), acetylated Spd (N8-acetyl-Spd) and acetylated spermine (N1-acetyl-Spm) in the transgenic Arabidopsis thaliana leaves. Heat shock induced increase of soluble-PAs (S-PAs) and soluble hydrolyzed-PAs (SH-PAs), especially of SH-Spm, and more importantly of acetylated Spd and Spm. The silencing of HSP90 genes or pharmacological inhibition of the HSP90 proteins by the specific inhibitor radicicol, under HS stimulatory conditions, resulted in a further increase of PA titers, N8-acetyl-Spd and N1-acetyl-Spm, and also stimulated the expression of PAO genes. The increased PA titers and PAO enzymatic activity resulted in a profound increase of PAO-derived hydrogen peroxide (H2O2) levels, which was terminated by the addition of the PAO-specific inhibitor guazatine. Interestingly, the loss-of-function Atpao3 mutant exhibited increased mRNA levels of selected AtHSP90 genes. Taken together, the results herein reveal a novel function of HSP90 and suggest that HSP90s and PAOs cross-talk to orchestrate PA acetylation, oxidation, and PA/H2O2 homeostasis.


Introduction
Heat stress impairs plant growth and productivity globally [1]. Plants, as sessile organisms, are highly adaptive to harsh environmental conditions and accommodate the developmental programming in a wide temperature range. Survival above optimal temperature conditions is accompanied by a massive accumulation of HSPs [2][3][4]. HSPs maintain and stabilize proteins and transcription factors through heterocomplex formation and folding/unfolding, thus controlling the activation/inhibition Impaired HSP90 action, derived either from the HSP90RNAi effect or from pharmacological inhibition of the HSP90 protein activity, induced increase in PA titers, especially of the acetylated-PAs, N 8 -acetyl-Spd and N 1 -acetyl-Spm and the conjugated forms. The interconnection between HSP90 and PAs was further determined since loss of PA homeostasis by impairing a major path of PA oxidation had a specific impact on HSP90 both at transcriptional and translational levels. Similarly, genetic modification of HSP90 had a profound effect on genes involved in the PA oxidation pathway. Our results reveal a novel interaction by which HSP90s/PAOs co-regulate the intracellular titers of PAs/H 2 O 2 with an unknown yet impact on stress response.

Underexpression of AtHSP90 1-4 Genes Results in Higher S-PAs and S-N 8 /N 1 Acetylated PA Titers
Polyamine homeostasis and PAO expression/PAO activity are cell/tissue/organ-specific, strongly affected by the ontogenetic stage of the plant/organ and the growing conditions [40,41]. In order to identify any potential link between HSP90 and PA homeostasis, the endogenous PA titers were analyzed in the leaves of 15 day-old WT, Athsp90-1 and Athsp90-4 mutants and pLFY:HSP90RNAi transgenic plants. More specifically, the titers of soluble Put (S-Put), S-Spd, S-Spm, and the two mono-acetylated PAs, N 8 -acetyl-Spd and N 1 -acetyl-Spm were determined ( Figure 1A). The leaves of Athsp90 1-4 contained significantly higher levels of S-Spm and acetyl-PAs and the pLFY:HSP90RNAi line contained higher levels of S-Put, S-Spd and S-Spm compared to WT. Interestingly, the pLFY:HSP90RNAi transgenics exhibited a nearly 18-fold increase of (N 8 -acetyl-Spd + N 1 -acetyl-Spm) compared to the WT ( Figure 1A).
To further confirm that genetic inhibition of the HSP90 genes affects PA homeostasis independently of plant age, PA contents were determined in 35 day-old mutants/transgenics and WT plants. Consistently, although a general decrease in PA levels was observed compared to the 15 day-old seedlings, in accordance with our previous results for tobacco and grapevine [40], all three lines, Athsp90 1-4, and pLFY:HSP90RNAi contained significantly greater S-Spm with the highest levels recorded in the pLFY:HSP90RNAi line, which also contained higher S-Put ( Figure 1B). The most striking effect of AtHSP90 deregulation on PA titers was, as in the 15 day-old plants, the significant increase of S-acetylated forms of Spd (N 8 -acetyl-Spd) and Spm (N 1 -acetyl-Spm) in the Athsp90 transgenic lines ( Figure 1B). The picture was somehow different in the conjugated SH-fraction ( Figure S1). The Athsp90 1-4 lines contained significantly higher SH-Put, SH-Spd and SH-Spm, as well as SH-acetyl-PAs, when compared to the corresponding titers in the S-fraction whereas the pLFY:HSP90RNAi line contained significantly lower amounts of SH-and acetyl-PAs, when compared to the Athsp90 1-4 mutants and to WT ( Figure S1). The results point strongly to a complex mechanism connecting HSP90 and PA homeostasis which may be ontogenetically regulated.  Values from 8 replicates from two independent experiments were pooled for statistical analysis. Asterisks indicate statistically significant differences from WT control (p < 0.05).

The Increased PAs in the Athsp90 Mutants Correlate with Increased H2O2 Content. HSP90s Modulate Free Radical Production
Under normal conditions, PA back-conversion catalyzed by PAOs leads to H2O2 production balancing the intracellular oxygen consumption rate [38]. In order to examine whether HSP90s modulate H2O2 production, the in situ semi-quantitative method based on staining with 3,3'diaminobenzidine (DAB) was employed. We found that all 15 day-old Athsp90 and pLFY:HSP90RNAi seedlings had increased H2O2 content compared to WT seedlings, as assessed by DAB staining (Figure 2). Hydrogen peroxide levels paralleled the increased S-PAs titers, which are substrates of PAOs; the pLFY:HSP90RNAi line as well as the Athsp90-1 and the Athsp90-4 young seedlings, which contained the highest S-and acetyl-PAs, exhibited the highest H2O2 levels compared to WT ( Figures 1A,2). These results further point to a certain correlation between HSP90s, PAs, and PAO-generated H2O2. L00F6ffelholz   [38]. In order to examine whether HSP90s modulate H 2 O 2 production, the in situ semi-quantitative method based on staining with 3,3'-diaminobenzidine (DAB) was employed. We found that all 15 day-old Athsp90 and pLFY:HSP90RNAi seedlings had increased H 2 O 2 content compared to WT seedlings, as assessed by DAB staining (Figure 2). Hydrogen peroxide levels paralleled the increased S-PAs titers, which are substrates of PAOs; the pLFY:HSP90RNAi line as well as the Athsp90-1 and the Athsp90-4 young seedlings, which contained the highest S-and acetyl-PAs, exhibited the highest H 2 O 2 levels compared to WT ( Figures 1A and 2). These results further point to a certain correlation between HSP90s, PAs, and PAO-generated H 2 O 2 .

Under HS, Genetic Depletion and Pharmacological Inhibition of HSP90 Affect PA Homeostasis and Enhance Acetylated Forms
Since HS induces transcription of the HSP90 genes [12,13,16], it was of interest to assess whether genetic depletion of HSP90 1-4 genes by RNAi [22] or pharmacological inhibition of HSP90 in WT Arabidopsis plants would similarly affect PA homeostasis under HS conditions. Thus, analysis of PA titers was performed in leaves of WT and pLFY:HSP90RNAi transgenic plants following acute HS (1 h at 42 ℃) in the presence (in WT) or absence (in pLFY:HSP90RNAi) of radicicol (Rad), a specific inhibitor of HSP90 activity. In WT, HS resulted in increased S-Spm, and considerable decrease of SH-Spd and SH-Spm levels ( Figure 3). Soluble-Spm is a common thermoresponsive indicator and has been proposed as a marker of thermotolerance [65]. The combined effect of HS + Rad resulted in increased S-Put, S-Spd, SH-Put, SH-Spd and SH-Spm when compared to HS treatment alone. In the WT, the S-N 8 -acetyl-Spd and N 1 -acetyl-Spm titers were slightly affected by HS, whereas the inhibition of HSP90 by Rad resulted in significant increase of the Nacetyl-PAs in both fractions S and SH ( Figure 3). Similarly, genetic depletion of cytosolic HSP90s in the pLFY:HSP90RNAi line resulted in a significant increase of all S-PAs, SH-PAs as well as of Sacetyl-PAs and SH-acetyl-PAs under HS. In particular, S-Spd in pLFY:HSP90RNAi showed a nearly 3-fold increase ( Figure 3). These data indicate that impairment of the HSP90 molecular chaperones, when coupled to the stimulatory effect of the HS-related pathway, increases further the deregulation of PA homeostasis and leads to a significant increase of acetylated PAs.

Under HS, Genetic Depletion and Pharmacological Inhibition of HSP90 Affect PA Homeostasis and Enhance Acetylated Forms
Since HS induces transcription of the HSP90 genes [12,13,16], it was of interest to assess whether genetic depletion of HSP90 1-4 genes by RNAi [22] or pharmacological inhibition of HSP90 in WT Arabidopsis plants would similarly affect PA homeostasis under HS conditions. Thus, analysis of PA titers was performed in leaves of WT and pLFY:HSP90RNAi transgenic plants following acute HS (1 h at 42 • C) in the presence (in WT) or absence (in pLFY:HSP90RNAi) of radicicol (Rad), a specific inhibitor of HSP90 activity. In WT, HS resulted in increased S-Spm, and considerable decrease of SH-Spd and SH-Spm levels ( Figure 3). Soluble-Spm is a common thermo-responsive indicator and has been proposed as a marker of thermotolerance [65]. The combined effect of HS + Rad resulted in increased S-Put, S-Spd, SH-Put, SH-Spd and SH-Spm when compared to HS treatment alone. In the WT, the S-N 8 -acetyl-Spd and N 1 -acetyl-Spm titers were slightly affected by HS, whereas the inhibition of HSP90 by Rad resulted in significant increase of the N-acetyl-PAs in both fractions S and SH ( Figure 3). Similarly, genetic depletion of cytosolic HSP90s in the pLFY:HSP90RNAi line resulted in a significant increase of all S-PAs, SH-PAs as well as of S-acetyl-PAs and SH-acetyl-PAs under HS. In particular, S-Spd in pLFY:HSP90RNAi showed a nearly 3-fold increase ( Figure 3). These data indicate that impairment of the HSP90 molecular chaperones, when coupled to the stimulatory effect of the HS-related pathway, increases further the deregulation of PA homeostasis and leads to a significant increase of acetylated PAs.

Genetic and Pharmacological Inhibitions of HSP90 Affect the PA Oxidation Pathway
Taking into consideration the changes in PA titers and the high levels of H2O2 in the RNAi pLFY:HSP90RNAi line (Figures 1,2), we studied the expression profile of AtPAO1, 3, 5 and AtHSP90 1-4 genes in WT and this transgenic line. To explore the impact that HSP90 could have on PA homeostasis, we studied the expression of AtPAO1, AtPAO3 and AtPAO5 genes in pLFY:HSP90RNAi plants in which cytosolic HSP90 proteins are markedly depleted [16,20]. In WT plants, HS increased mRNA levels of AtHSP90-1 and AtHSP90-4; treatment with HS + Rad also induced high AtHSP90-1 and AtHSP90-4 transcript levels when compared to the controls. It is worth noticing that the abundance of AtHSP90-1 is slightly lower when HS coupled with Rad than HS alone ( Figure 4A).
AtPAO mRNAs in the pLFY:HSP90RNAi lines were increased when compared to WT under control conditions, which was not remarkably different under HS ( Figure 4B). If the expression of PAO genes was affected by HSP90 function, then Rad should modulate gene transcription pattern

Genetic and Pharmacological Inhibitions of HSP90 Affect the PA Oxidation Pathway
Taking into consideration the changes in PA titers and the high levels of H 2 O 2 in the RNAi pLFY:HSP90RNAi line (Figures 1 and 2), we studied the expression profile of AtPAO1, 3, 5 and AtHSP90 1-4 genes in WT and this transgenic line. To explore the impact that HSP90 could have on PA homeostasis, we studied the expression of AtPAO1, AtPAO3 and AtPAO5 genes in pLFY:HSP90RNAi plants in which cytosolic HSP90 proteins are markedly depleted [16,20]. In WT plants, HS increased mRNA levels of AtHSP90-1 and AtHSP90-4; treatment with HS + Rad also induced high AtHSP90-1 and AtHSP90-4 transcript levels when compared to the controls. It is worth noticing that the abundance of AtHSP90-1 is slightly lower when HS coupled with Rad than HS alone ( Figure 4A).
AtPAO mRNAs in the pLFY:HSP90RNAi lines were increased when compared to WT under control conditions, which was not remarkably different under HS ( Figure 4B). If the expression of PAO genes was affected by HSP90 function, then Rad should modulate gene transcription pattern similarly to genetically compromised HSP90 activity. In fact, Rad application on WT plants induced the expression of the AtPAO1, AtPAO3 and AtPAO5 genes ( Figure 4B). Consequently, pharmacological HSP90 inactivation affected the expression of PAO genes in a similar way to HSP90 mRNA depletion by RNAi silencing. Similar results were obtained when the abundance of PAO3 protein was assessed. The levels of PAO3 protein correlated well with the corresponding mRNA levels in both the WT and the pLFY:HSP90RNAi plants under the different treatments ( Figure 4C). similarly to genetically compromised HSP90 activity. In fact, Rad application on WT plants induced the expression of the AtPAO1, AtPAO3 and AtPAO5 genes ( Figure 4B). Consequently, pharmacological HSP90 inactivation affected the expression of PAO genes in a similar way to HSP90 mRNA depletion by RNAi silencing. Similar results were obtained when the abundance of PAO3 protein was assessed. The levels of PAO3 protein correlated well with the corresponding mRNA levels in both the WT and the pLFY:HSP90RNAi plants under the different treatments ( Figure 4C).

Inhibition of HSP90 under HS Induces Expression of NATA1, a Putative Acetyltransferase-like Gene
As shown above, leaves of pLFY:HSP90RNAi plants exposed to HS as well as leaves of WT exposed to HS coupled with Rad, exhibit a noticeable increase of acetylated forms of higher PAs ( Figure 3). The Arabidopsis NATA genes are the closest homologues of mammalian PA acetyltransferases. Thus, due to the lack of identified genes regulating the acetylation of Spd and Spm in Arabidopsis, we prompted to examine the transcript levels of NATA1 gene (At2g39030) which is identified as a Put and ornithine acetyltransferase [67,68], as a means to assess the genetic trend of PA acetylation under our conditions. Again, and by using excised leaves incubated in Morpholino Ethane Sulfonic acid (MES) and exposed to HS with (WT)/without Rad (Athsp90s), the WT and the PLFY:HSP90RNAi mutant under control conditions contained low amounts of NATA1 (B) a. Abundance of transcripts of AtPAO1, AtPAO3 and AtPAO5, b. relative pixels density of gel bands' intensities following normalization with AtUBQ, c. NATA1 mRNA levels in WT and pLFY:HSP90RNAi mutant, with relative densitometric measurements following normalization with AtUBQ. (C) PAO3 immunoreactive protein relative levels in the leaves under control, HS and HS + Rad conditions. Image software was used for numeric determination and quantification of gels bands' intensities.

Inhibition of HSP90 under HS Induces Expression of NATA1, a Putative Acetyltransferase-Like Gene
As shown above, leaves of pLFY:HSP90RNAi plants exposed to HS as well as leaves of WT exposed to HS coupled with Rad, exhibit a noticeable increase of acetylated forms of higher PAs ( Figure 3). The Arabidopsis NATA genes are the closest homologues of mammalian PA acetyltransferases. Thus, due to the lack of identified genes regulating the acetylation of Spd and Spm in Arabidopsis, we prompted to examine the transcript levels of NATA1 gene (At2g39030) which is identified as a Put and ornithine acetyltransferase [67,68], as a means to assess the genetic trend of PA acetylation under our conditions. Again, and by using excised leaves incubated in Morpholino Ethane Sulfonic acid (MES) and exposed to HS with (WT)/without Rad (Athsp90s), the WT and the PLFY:HSP90RNAi mutant under control conditions contained low amounts of NATA1 mRNA. Heat shock induced NATA1 expression in the WT and PLFY:HSP90RNAi plants, exhibiting a 2-fold increase of the transcript levels. Interestingly, in WT plants NATA1 transcripts increased more than 5-fold under HS in the presence of Rad. The results show that inhibition of HSP90 by the HSP inhibitor Rad releases PA acetylation process and suggest that PA acetylation is in fact inhibited by HSP90 ( Figure 4B,C).

PAO-Induced Stimulation during HSP90 Inhibition Is Responsible for the Increased H 2 O 2
Since inhibition of HSP90 induced PAO transcription and increased PAO protein levels ( Figure 4B,C), supporting cross-talk between the inhibition of HSP90 and the stimulation of the PAO  Figure 5A and Figure  S2A). To further test this finding, we also determined PAO activity in planta intact WT leaves infiltrated with Rad or Spd (as a positive control) and submitted to HS ( Figure 5B). In addition, the results were confirmed by determining the endogenous H 2 O 2 of leaves treated as above, which confirmed that in fact high PAO activity correlates with high H 2 O 2 levels ( Figure 5C). Finally, the increased amounts of H 2 O 2 were further verified by in situ staining of WT leaves under HS and HS + Rad. Consistently, guazatine (specific PAO inhibitor), when administered simultaneously with Rad, reduced H 2 O 2 levels, supporting that the higher PAO activity contributes to H 2 O 2 generation upon HSP90 inhibition ( Figure 5D). As with WT, the use of guazatine along with HS attenuated PAO activity in PLFY:HSP90RNAi leaves ( Figure S3A,B). Excised WT leaves cultured on MES medium and exposed to HS with/without Rad; and excised pLFY:HSP90RNAi line leaves cultured on MES medium and exposed to HS. (B) In planta WT leaves infiltrated separately with water (control or HS); Rad, and Spd (as positive control) prior to exposure to HS. (C) Luminometric hydrogen peroxide quantification in WT leaves exposed to HS and HS + Rad, and pLFY:HSP90RNAi mutant exposed to HS. (D) In situ DAB staining of hydrogen peroxide in WT control leaves treated with HS and HS + Rad and with HS + Rad + guazatine (Guaz). Values from 8 replicates from two independent experiments were pooled for statistical analysis. Asterisks indicate statistically significant differences from WT control (p < 0.05).

PAO and AtHSP90 Reciprocally Affect each Other's Expression
To examine whether PAO homeostasis has an impact on AtHSP90 expression, Atpao3 loss-offunction plants were tested. By applying HS to leaves incubated in MES medium, transcript analysis revealed that only AtHSP90-1 and AtHSP90-4 mRNAs were increased in the Atpao3 mutant compared to WT. The abundance of AtHSP90 1-4 mRNAs as well as HSP90 protein were analyzed Figure 5. Inhibition of HSP90 stimulates the polyamine oxidation activity. Polyamine Oxidase (PAO) activity measured and evaluated through hydrogen peroxide production in: (A) Excised WT leaves cultured on MES medium and exposed to HS with/without Rad; and excised pLFY:HSP90RNAi line leaves cultured on MES medium and exposed to HS. (B) In planta WT leaves infiltrated separately with water (control or HS); Rad, and Spd (as positive control) prior to exposure to HS. (C) Luminometric hydrogen peroxide quantification in WT leaves exposed to HS and HS + Rad, and pLFY:HSP90RNAi mutant exposed to HS. (D) In situ DAB staining of hydrogen peroxide in WT control leaves treated with HS and HS + Rad and with HS + Rad + guazatine (Guaz). Values from 8 replicates from two independent experiments were pooled for statistical analysis. Asterisks indicate statistically significant differences from WT control (p < 0.05).

PAO and AtHSP90 Reciprocally Affect Each Other's Expression
To examine whether PAO homeostasis has an impact on AtHSP90 expression, Atpao3 loss-of-function plants were tested. By applying HS to leaves incubated in MES medium, transcript analysis revealed that only AtHSP90-1 and AtHSP90-4 mRNAs were increased in the Atpao3 mutant compared to WT. The abundance of AtHSP90 1-4 mRNAs as well as HSP90 protein were analyzed in the Atpao3 mutant and the WT, under HS ( Figure 6A,B). In the Atpao3, mRNA titers of the AtHSP90-1 and AtHSP90-4 were significantly greater compared to WT, whilst HS resulted in an obvious increase of the mRNA levels of the AtHSP90 1-4 genes ( Figure 6A). Inhibition of HSP90 activity simultaneously to HS stimulation (HS + Rad) exerted no further significant effect on the AtHSP90 1-4 mRNA levels ( Figure 6A). It is known that HSP90 are essential molecular chaperones in eukaryotic cells, with key functions in signal transduction networks. To test whether HSP90 homeostasis is disrupted when the PA oxidation pathway is challenged, the molecular chaperone protein abundance was assessed. Immunoreactive HSP90 protein levels exhibited parallel increase under HS in both, WT and Atpao3 and was more abundant in the Atpao3 genotype ( Figure 6B,S2B). In pao3 mutant, the HSP90 protein levels were increased. Furthermore, semi-quantitative reverse transcriptase (RT)-PCR revealed that the cytosolic AtHSP90 1-4 genes in Atpao3 seedlings (5, 10, and 15 day-old) revealed a general over-expression of these genes in the Atpao3 mutant compared to WT, at different time points ( Figure S4).
Interestingly, the Atpao3 leaves contained lower endogenous mRNA levels of all four AtPAO genes (AtPAO1, 2, 4 and 5). Pharmacological inhibition of HSP90 activity under HS (HS + Rad) induced an increase in the AtPAO transcripts and restored the AtPAO mRNAs to levels similar to the WT control, except the AtPAO4 that remained low ( Figure S5A). The restoration of the AtPAO mRNA levels in the Atpao3 mutant subsequently to the inhibitory effect of Rad on HSP90 activity suggests that AtPAOs are activated subsequently to HSP90 deregulation. Application of HS + Rad did not induce accumulation of acetyl-S-PAs in the Atpao3 mutant genotype ( Figure S5B).

Discussion
The hypothesis of cross-talk between HSPs and PAs was initially evoked through the finding that exogenous Spm increases mRNA levels of HSP90 and subsequently enhances heat tolerance in Arabidopsis [65]. Results herein support this hypothesis and reveal reciprocal molecular and biochemical interactions of HSP90/PAO genes and/or HSP90/PAO proteins. Depletion of cytoplasmic HSP90 results in increased levels of PAs and more specifically of N 8 -acetyl-Spd and N 1acetyl-Spm, which, under physiological conditions, are present only in traces in the leaves of WT (Figure 1) [69]. In animals, Spd/Spm acetyltransferase (SSAT) is a key enzyme participating in the first step of PA catabolism, mediating the acetylation of PAs whereas PAOs catalyze the final oxidation of acetyl-PAs. Overexpression of SSAT correlates with growth inhibition in a wide range It is known that HSP90 are essential molecular chaperones in eukaryotic cells, with key functions in signal transduction networks. To test whether HSP90 homeostasis is disrupted when the PA oxidation pathway is challenged, the molecular chaperone protein abundance was assessed. Immunoreactive HSP90 protein levels exhibited parallel increase under HS in both, WT and Atpao3 and was more abundant in the Atpao3 genotype ( Figure 6B and Figure S2B). In pao3 mutant, the HSP90 protein levels were increased.
Furthermore, semi-quantitative reverse transcriptase (RT)-PCR revealed that the cytosolic AtHSP90 1-4 genes in Atpao3 seedlings (5, 10, and 15 day-old) revealed a general over-expression of these genes in the Atpao3 mutant compared to WT, at different time points ( Figure S4).
Interestingly, the Atpao3 leaves contained lower endogenous mRNA levels of all four AtPAO genes (AtPAO1, 2, 4 and 5). Pharmacological inhibition of HSP90 activity under HS (HS + Rad) induced an increase in the AtPAO transcripts and restored the AtPAO mRNAs to levels similar to the WT control, except the AtPAO4 that remained low ( Figure S5A). The restoration of the AtPAO mRNA levels in the Atpao3 mutant subsequently to the inhibitory effect of Rad on HSP90 activity suggests that AtPAOs are activated subsequently to HSP90 deregulation. Application of HS + Rad did not induce accumulation of acetyl-S-PAs in the Atpao3 mutant genotype ( Figure S5B).

Discussion
The hypothesis of cross-talk between HSPs and PAs was initially evoked through the finding that exogenous Spm increases mRNA levels of HSP90 and subsequently enhances heat tolerance in Arabidopsis [65]. Results herein support this hypothesis and reveal reciprocal molecular and biochemical interactions of HSP90/PAO genes and/or HSP90/PAO proteins. Depletion of cytoplasmic HSP90 results in increased levels of PAs and more specifically of N 8 -acetyl-Spd and N 1 -acetyl-Spm, which, under physiological conditions, are present only in traces in the leaves of WT (Figure 1) [69]. In animals, Spd/Spm acetyltransferase (SSAT) is a key enzyme participating in the first step of PA catabolism, mediating the acetylation of PAs whereas PAOs catalyze the final oxidation of acetyl-PAs. Overexpression of SSAT correlates with growth inhibition in a wide range of tumors [70][71][72]. Accumulation of acetyl-PAs by the increased activity of SSAT shifts the metabolism of PAs into lipids and carbohydrates through the increasing consumption of acetyl-CoA and ATP, leading to generation of ROS and, subsequently, to oxidative stress and proteotoxicity [71]. On the contrary, the acetyl-PAs are not the preferred substrates for PAOs in plants [39,55]. Acetylated-Put is directly linked to the pathogen-defense related signals [67] as well as the root cell de-differentiation process, probably as transient metabolite for GABA production [73]. The factors controlling the tri-and tetraamine (Spd and Spm) acetylation remain obscure. NATA1 is one of the few genes participating in the N-acetylation of amine metabolism in Arabidopsis; ornithine is the favorite substrate when stimulated via methyl jasmonate [67] and Put as specific substrate when stimulated through jasmonate and salicylic acid [68], both involving the pathogen infection process. Analysis of the mRNA levels of the NATA1 in pLFY:HSP90RNAi HS-treated leaves versus WT treated with HS +/-Rad confirms the stimulation of the NATA1 transcription under HS in both genotypes, and a further stimulation when HS is associated with HSP90 inhibitor in the WT ( Figure 4B).
Both, HSP90s and PAs, are highly responsive to environmental challenges. HSP90s increase under stress, including heat, osmotic, and heavy metals, as well as biotic stress, whereas PAs exhibit complex profiles under such conditions [1,12,32,37,38,[74][75][76]. Spermine plays a significant role in Arabidopsis thermotolerance, and exogenously applied Spm enhances HS response by inducing increase of mRNA levels of HSP genes [65]. Although sufficient information on the implication of HSP in proteotoxicity is lacking, it is well established that HSP chaperones are highly responsible for the prevention of protein degradation and misfolding and for preventing non-native proteins from non-specific intermolecular interactions, eliminating proteotoxicity propagation [77,78]. Nishizawa-Yokoi et al. [79] propose a model for HSP90s role in ROS-induced proteotoxic stress in Arabidopsis thaliana. They suggest that ROS accumulation results in inhibition of proteasome 26S, which induces a peculiar poly-ubiquitination. HSP90s, which act as Heat Shock Factor (HSF) repressors under normal conditions, release the HSF which regulates in turn the expression of a Heat Shock Transcription Factor A2 (HSFA2). Also, HSP90s regulate the production of superoxides mediated by NADPH-oxidase but are not required for the generation of H 2 O 2 in human embryonic kidney cells [75]. Pharmacological inhibition of HSP90 in PC-12 cells induces cytotoxicity and cell death via oxidative stress by an unidentified mechanism [34]. Taking into consideration the involvement of HSP90 in cell proliferation processes, we can speculate that both, HSP90s and PAs may be integrated in a specific biochemical pathway through the competitive regulation of cell cytotoxicity and ROS generation involving potentially the homeostatic regulation of oxidation and/or acetylation of PAs.
The drastic reduction of HSP90s results in deregulation of PA titers and induction of the PAO pathway, which generates H 2 O 2 . In WT, the pharmacological inhibition of HSP90s under HS induces expression of AtPAO genes, suggesting that HSP90s may be implicated in a way to decelerate the action of PAOs into the cell. Indeed, the pLFY:HSP90RNAi line exhibits elevated endogenous AtPAO mRNA levels similar to those in WT when treated with HS + Rad, suggesting that biochemical inhibition of HSP90s defines a favorable endogenous potential of PA oxidation (Figures 4 and 5).
As key agents in the regulation of thermotolerance, HSP90s show strong connection with other stress-related factors in signal perception and transduction [18,80]. Within this context, HS induces in both WT and pLFY:HSP90RNAi mutant an increase of H 2 O 2 , which is a predictable trait of stress ( Figure 5C). Simultaneous application of HS + Rad enhances H 2 O 2 generation in WT. In parallel with the induction of PA oxidation, the use of guazatine, a specific inhibitor of PAO, attenuates the HS + Rad-dependent accumulation of H 2 O 2 , confirming the concomitant link between HSP90 inhibition/PA oxidation-back-conversion/H 2 O 2 generation. HSP90 inhibition results in AtPAOs expression and increased transcript levels, especially the AtPAO1 and AtPAO5. Within this context and the specific affinity of the two proteins AtPAO1 and AtPAO5 to Spm (Nor and especially T-Spm) [52,81], we stress the specific implication of Spm oxidation in HS response. AtPAO3 is also highly affected by the deletion of the HSP90 action but seems to follow different pattern. Furthermore, the transcriptional analysis of the four cytosolic AtHSP90 genes in the Atpao3 mutant reveals the high endogenous AtHSP90 transcript levels in this mutant and once again the putative mutual inhibitory effect.
Overall, our results suggest a link between HSP90s and PAs/PAOs/H 2 O 2 under HS. As schematically presented in the proposed model (Figure 7), HSP90 proteins participate in the regulation of PA conjugation, more specifically, in PA acetylation. The pLFY:HSP90RNAi plants exhibit: (i) overexpression of the major AtPAO genes and consequently increased PAO protein and activity levels, which result in increased H 2 O 2 intrinsic levels; and (ii) significant increase of PAs and, especially, of acetylated higher PAs titers. In turn, underexpression of the AtPAO3 gene (Atpao3 mutant), induces overexpression of one or more of the AtHSP90 1-4 genes. Under conditions of HS as pivotal HSP90 stimulator: (1) the transgenic reduction of the HSP90 genes (pLFY:HSP90RNAi), as well as the pharmacological inhibition of the HSP90 proteins via Rad affect similarly both pathways. Namely: (i) positive regulation of the AtPAOs and further increase of the PAOs-generated H 2 O 2 , which is also observed under HS conditions in the WT, but to a lesser extent, suggesting that HSP90s are involved in the regulation of the stress-induced PA-oxidation/back-conversion pathway; and, (ii) increase of PAs and acetylated-higher PAs, showing a specific heat-related HSP90 pathway as revealed by HSP90 inhibition. Consequently, by being powerful modulators of PAOs, HSP90s affect the PAO-generated H 2 O 2 , which participates in the stress-induced downstream signaling cascade that is tidily related to ROS homeostasis and PCD [38,43,50,51,61,82,83].  HSP90RNAi), as well as the pharmacological inhibition of the HSP90 proteins via Rad affect similarly both pathways. Namely: (i) positive regulation of the AtPAOs and further increase of the PAOs-generated H2O2, which is also observed under HS conditions in the WT, but to a lesser extent, suggesting that HSP90s are involved in the regulation of the stress-induced PA-oxidation/backconversion pathway; and, (ii) increase of PAs and acetylated-higher PAs, showing a specific heatrelated HSP90 pathway as revealed by HSP90 inhibition. Consequently, by being powerful modulators of PAOs, HSP90s affect the PAO-generated H2O2, which participates in the stressinduced downstream signaling cascade that is tidily related to ROS homeostasis and PCD [38,43,50,51,61,82,83]. Taken all together, these results reveal a mutually antagonistic HS-regulated effect involving HSP90s, PAs, PAOs and H2O2 in Arabidopsis thaliana.

Plant Material and Treatments
Arabidopsis thaliana (ecotype Col-0, WT), an pLFY:HSP90RNAi silencing line against the four cytosolic AtHSP90 genes [22], two T-DNA insertion mutant lines for the Athsp90-1 and Athsp90-4 Taken all together, these results reveal a mutually antagonistic HS-regulated effect involving HSP90s, PAs, PAOs and H 2 O 2 in Arabidopsis thaliana.

Plant Material and Treatments
Arabidopsis thaliana (ecotype Col-0, WT), an pLFY:HSP90RNAi silencing line against the four cytosolic AtHSP90 genes [22], two T-DNA insertion mutant lines for the Athsp90-1 and Athsp90-4 (SALK_007614 and SALK_084059, respectively) and a T-DNA loss-of-function Atpao3 (SALK_121288) line were used. In vitro produced seedlings were germinated on 1 2 MS medium [84] supplemented with 0.5% sucrose on Petri dishes and plants were cultured in pots in a controlled growth chamber (23 • C and 8/16 h photoperiod under 150 µmol m −2 s −1 ). Fully expanded leaves were excised from 4-week-old vegetative plants, before the floral transition phase, incubated in MES medium (pH 5.7) with agitation for 24 h, and subjected to different treatments: controls were incubated at 23 • C and HS samples at 42 • C, in the presence or absence of 10 mM Spd, 50 µM guazatine (specific inhibitor of PAO), 300 nM radicicol (specific inhibitor of HSP90) or 10 mM H 2 O 2 . Each chemical was added to the medium accompanied by brief vacuum treatment, 10 min prior to HS. All experiments were performed twice in identical growth chambers and growth conditions, with 4 replicates per treatment randomly designed. Samples were collected after 1 h treatment. Eight replicates from two independent experiments were pooled for statistical analysis. Asterisks indicate statistically significant differences from the WT control (p < 0.05).

Construction of HSP90-RNAi Line (pLFY:HSP90RNAi Mutant)
The construction of the HSP90-RNAi line is described in [22]. In this work, we analyzed the pLFY:HSP90RNAi mutant, line 10.1
For the semi-Q RT-PCR, 5, 10 and 15 day-old seedlings of Arabidopsis wild type (Col) and Atpao3 were used. First strand cDNA was synthesized using total RNA from each plant sample using SuperScript II Reverse Transcriptase (Invitrogen) in a 20 µL reaction. PCR amplification for each transcript (AtHSP90-1: 392 bp, AtHSP90-2: 192 bp, AtHSP90-3: 2531 bp and the AtHSP90-4: 128 bp) was performed using the gene-specific primers (Table S1). RT-PCR and semi-Q RT-PCR reactions were normalized using the Ubq and 18S rDNA Arabidopsis genes, respectively [86]. For all semi-Q RT-PCRs, three technical replicates were prepared from materials pooled from all biological replicates/experiments. At least three variations in cycle number were used to verify reproducibility and amplification in the logarithmic phase, respectively. To estimate the amount of PCR product obtained, PCR reactions were run on ethidium bromide-containing agarose gels and subsequently analyzed in UV light. During gel analysis and data collection, volumes and settings were chosen to avoid saturation of fluorescence signals. RT and semi-Q RT-PCR products were quantified by measuring the intensity per unit area with Image J. The gene specificity of RT-PCR products was confirmed by sequencing. Data represent the mean of the technical replicates.

Determination and In Situ Localization of Hydrogen Peroxide
Hydrogen peroxide was quantified via a chemiluminescence assay [87]. In situ H 2 O 2 was detected by DAB staining using the method of Thordal et al. [88].

Extraction and Quantification of PAs
Polyamines were extracted and analyzed according to Kotzabasis et al. [89], using an HP 1100 high performance liquid chromatographer (HPLC; Hewlett-Packard, Wadbronn, Germany).

Protein Extraction and Enzyme Assays
Total proteins were extracted as described in Papadakis and Roubelakis-Angelakis [90]. Protein content was measured according to the Lowry method [91]. PAO activity was measured according to the method of Yoda et al. [43] with minor modifications; fifty µg protein extract were incubated with 10 mM Spd for 30 min and measurement of generated H 2 O 2 was performed for 1 min using a luminometer in a Tris-HCl (pH 8.0) reaction buffer containing 125 mM luminol [92].

Image and Statistical Analysis
Image analysis was performed using ImageJ v 1.41 software, and statistical analysis using one-way ANOVA and the Duncan's test in the STATISTICA software.

Conflicts of Interest:
The authors declare no conflict of interest.