Post-Translational Modification of Proteins Mediated by Nitro-Fatty Acids in Plants: Nitroalkylation

Nitrate fatty acids (NO2-FAs) are considered reactive lipid species derived from the non-enzymatic oxidation of polyunsaturated fatty acids by nitric oxide (NO) and related species. Nitrate fatty acids are powerful biological electrophiles which can react with biological nucleophiles such as glutathione and certain protein–amino acid residues. The adduction of NO2-FAs to protein targets generates a reversible post-translational modification called nitroalkylation. In different animal and human systems, NO2-FAs, such as nitro-oleic acid (NO2-OA) and conjugated nitro-linoleic acid (NO2-cLA), have cytoprotective and anti-inflammatory influences in a broad spectrum of pathologies by modulating various intracellular pathways. However, little knowledge on these molecules in the plant kingdom exists. The presence of NO2-OA and NO2-cLA in olives and extra-virgin olive oil and nitro-linolenic acid (NO2-Ln) in Arabidopsis thaliana has recently been detected. Specifically, NO2-Ln acts as a signaling molecule during seed and plant progression and beneath abiotic stress events. It can also release NO and modulate the expression of genes associated with antioxidant responses. Nevertheless, the repercussions of nitroalkylation on plant proteins are still poorly known. In this review, we demonstrate the existence of endogenous nitroalkylation and its effect on the in vitro activity of the antioxidant protein ascorbate peroxidase.

Polyunsaturated fatty acids are targets of peroxidation due to their unsaturated double bonds [4,11]. The main mechanisms of PUFA peroxidation are non-enzymatic autocatalytic oxidation reactions [1,12], while enzymatic oxidation reactions involving three heme-containing metallo-enzyme families (lipoxygenases (LOXs), cyclooxigenases (COXs) [1,13], and cytochromes P450 (CYPs) [1]), as well as NADP + -dependent dehydrogenases [1,14] which can also occur. Non-enzymatic mechanisms include PUFA nitration triggered by reactive nitrogen species (RNS) such as nitric oxide (NO) and its derived molecules [1,15,16]. A preferential target for lipid peroxidation is arachidonic acid, whose In plant systems, PUFA peroxidation caused by non-enzymatic or/and enzymatic (LOX-mediated) reactions generates some products with cytotoxic effects and others with protective anti-stress effects. The LOX pathway yields RLS related to plant defense responses to pathogen infections [40] and wounding [41], and in the regulation of hypersensitive programmed cell death [42] and senescence [43]. Non-enzymatic processes can generate both harmful products with damaging actions [44] and phytoprostanes, which have biological properties similar to those of jasmonic acid [45]. Recent knowledge has illustrated the formation of RLS that perform signaling roles and are implicated in antioxidant responses as a result of the oxidation of NO-derived molecules [9].
This review focuses on the study of reactive lipids species called nitroalkenes. Specifically, we will argue the biological properties of nitroalkenes both in animal and plant systems, as well as their signaling potential generated by a post-translational modification of proteins called nitroalkylation.

Nitro-Fatty Acids in Animals
The reactive lipids species resulting from the interaction of unsaturated fatty acids with NO and derived species, such as NO 2 and ONOO − , are called nitro-fatty acids (NO 2 -FAs), nitrolipids or nitroalkenes [46].
Although the interaction between unsaturated fatty acids and RNS has been widely studied, two distinct mechanisms have been suggested to explain the in vivo nitration of fatty acids, a process which remains unknown [47]. One mechanism involves the generation of an alkyl radical through a radical hydrogen abstraction from a bis-allylic carbon followed by a double-bond rearrangement and the incorporation of a NO 2 radical ( Figure 1A) [48,49]. The other mechanism consists on the generation of a carbon-centered radical through the direct addition of NO 2 , which can be further oxidized either with or without a second insertion of NO 2 in order to form the nitro-fatty acid. When the carbon-centered radical reacts with the second NO 2 , an unstable nitro-nitrite or dinitro compound appears which rapidly decomposes and releases nitrous acid (HNO 2 ), yielding the nitro-fatty acid ( Figure 1B) [49,50]. In plant systems, PUFA peroxidation caused by non-enzymatic or/and enzymatic (LOX-mediated) reactions generates some products with cytotoxic effects and others with protective anti-stress effects. The LOX pathway yields RLS related to plant defense responses to pathogen infections [40] and wounding [41], and in the regulation of hypersensitive programmed cell death [42] and senescence [43]. Non-enzymatic processes can generate both harmful products with damaging actions [44] and phytoprostanes, which have biological properties similar to those of jasmonic acid [45]. Recent knowledge has illustrated the formation of RLS that perform signaling roles and are implicated in antioxidant responses as a result of the oxidation of NO-derived molecules [9].
This review focuses on the study of reactive lipids species called nitroalkenes. Specifically, we will argue the biological properties of nitroalkenes both in animal and plant systems, as well as their signaling potential generated by a post-translational modification of proteins called nitroalkylation.

Nitro-Fatty Acids in Animals
The reactive lipids species resulting from the interaction of unsaturated fatty acids with NO and derived species, such as NO2 and ONOO − , are called nitro-fatty acids (NO2-FAs), nitrolipids or nitroalkenes [46].
Although the interaction between unsaturated fatty acids and RNS has been widely studied, two distinct mechanisms have been suggested to explain the in vivo nitration of fatty acids, a process which remains unknown [47]. One mechanism involves the generation of an alkyl radical through a radical hydrogen abstraction from a bis-allylic carbon followed by a double-bond rearrangement and the incorporation of a NO2 radical ( Figure 1A) [48,49]. The other mechanism consists on the generation of a carbon-centered radical through the direct addition of NO2, which can be further oxidized either with or without a second insertion of NO2 in order to form the nitro-fatty acid. When the carbon-centered radical reacts with the second NO2, an unstable nitro-nitrite or dinitro compound appears which rapidly decomposes and releases nitrous acid (HNO2), yielding the nitro-fatty acid ( Figure 1B) [49,50].  (B) NO 2 -FA formation by the direct addition of NO 2 and its oxidation (modified from Reference [49]).
In recent years, important progress in the endogenous detection of NO 2 -FAs has been achieved in animal and human models. In animal systems, it is worth highlighting the detection of nitrated oleic (NO 2 -OA) and linoleic acid (NO 2 -LA) in the murine model of focal cardiac ischemia-reperfusion (I/R). The formation of these NO 2 -FAs was due to reoxygenation-induced tissue damage which generated acidification, hypoxia, as well as ROS and RNS [49,51]. It should be mentioned that other NO 2 -FAs were detected in an experimental rat model of ischemic preconditioning (IPC) [49,52]. High-resolution liquid chromatography mass spectrometry (LC-MS/MS) procedures have revealed a preferential nitration of conjugated linoleic acid (cLA) in animal systems. This fatty acid presents positional and geometric isomers of linoleic acid which have conjugated dienes in cis and trans configurations. These species have conjugated double bonds which are not separated by a methylene group [53]. Conjugated linoleic acid, which displays more reactivity with · NO 2 than bis-allylic fatty-acids, is the main in vivo endogenous nitration target [47,49]. The formation of nitrated cLA has been detected in activated macrophages under inflammatory conditions and in the gastric compartment following the ingest of cLA and NO 2 − [47,49,54].
Advances in chromatography mass spectrometry techniques, in vitro nitration, and animal model studies have increased our understanding of the nitration of unsaturated fatty acids in humans. Dietary products such as oils and seeds are the principal sources of unsaturated fatty acids such as oleic acid (OA), conjugated linoleic (cLA), and linolenic (cLn) acids. Pomegranates are regarded as sources of cLn, while dairy products and meat are a source of cLA. Interestingly, cLA is absorbed at much higher levels than cLn [49,55]. Dietary products such as vegetables and herbs are sources of nitrate (NO 3 − ) and nitrite (NO 2 − ) [49,56,57]. These NO-derived species are necessary to generate nitrated PUFAs, as nitrite is a nitrating compound derived from nitrate. However, the low level of nitrite in basal metabolic conditions is increased through the conversion of nitrate by commensal bacteria in the gastrointestinal tract [58]. As with animal models, NO 2 -cLA is the principal nitroalkene generated in humans (Table 1) [47].
Nitrate fatty acids are endowed with a specific chemical reactivity which facilitates cellular signaling events. In addition, these molecules have potent biological properties such as a NO-releasing capacity which was observed for the first time in aqueous milieu in animal systems [15,[61][62][63]. Two possible NO-releasing mechanisms have been proffered. The first one consists of a modified Nef reaction which generates a nitrous intermediate with an especially weak C-N bond that quickly decays to yield NO and a radical stabilized by conjugation with alkene and the OH group ( Figure 2) [15,46]. The second mechanism involves the rearrangement of the nitroalkene to a nitrite ester followed by a process of homolysis to form NO and an enol group ( Figure 3) [46,64,65]. Another biological property of these compounds is their hydrophobic stability in cell membranes and lipoproteins, which act as endogenous NO 2 -FA reservoirs which can be supplied to other locations in the cell in order to act as signaling molecules [15]. An additional biological property of NO 2 -FAs is their capacity to mediate post-translational modifications through nitroalkylation, which will be discussed below [46,51,[66][67][68]. In addition to those mentioned above, other NO2-FAs, such as nitro-oleic acid (NO2-OA), nitro-linoleic acid (NO2-LA), conjugated nitro-linoleic acid (NO2-cLA), nitro-arachidonic acid (NO2-AA), and cholesteryl nitrolinoleate (NO2-CL) have been detected in vivo through quantitative analyses of blood and urine under both healthy and inflammatory conditions (Table 1) [59,60].
Nitrate fatty acids are endowed with a specific chemical reactivity which facilitates cellular signaling events. In addition, these molecules have potent biological properties such as a NO-releasing capacity which was observed for the first time in aqueous milieu in animal systems [15,[61][62][63]. Two possible NO-releasing mechanisms have been proffered. The first one consists of a modified Nef reaction which generates a nitrous intermediate with an especially weak C-N bond that quickly decays to yield NO and a radical stabilized by conjugation with alkene and the OH group ( Figure 2) [15,46]. The second mechanism involves the rearrangement of the nitroalkene to a nitrite ester followed by a process of homolysis to form NO and an enol group ( Figure 3) [46,64,65]. Another biological property of these compounds is their hydrophobic stability in cell membranes and lipoproteins, which act as endogenous NO2-FA reservoirs which can be supplied to other locations in the cell in order to act as signaling molecules [15]. An additional biological property of NO2-FAs is their capacity to mediate post-translational modifications through nitroalkylation, which will be discussed below [46,51,[66][67][68].  In addition to those mentioned above, other NO2-FAs, such as nitro-oleic acid (NO2-OA), nitro-linoleic acid (NO2-LA), conjugated nitro-linoleic acid (NO2-cLA), nitro-arachidonic acid (NO2-AA), and cholesteryl nitrolinoleate (NO2-CL) have been detected in vivo through quantitative analyses of blood and urine under both healthy and inflammatory conditions (Table 1) [59,60].
Nitrate fatty acids are endowed with a specific chemical reactivity which facilitates cellular signaling events. In addition, these molecules have potent biological properties such as a NO-releasing capacity which was observed for the first time in aqueous milieu in animal systems [15,[61][62][63]. Two possible NO-releasing mechanisms have been proffered. The first one consists of a modified Nef reaction which generates a nitrous intermediate with an especially weak C-N bond that quickly decays to yield NO and a radical stabilized by conjugation with alkene and the OH group ( Figure 2) [15,46]. The second mechanism involves the rearrangement of the nitroalkene to a nitrite ester followed by a process of homolysis to form NO and an enol group ( Figure 3) [46,64,65]. Another biological property of these compounds is their hydrophobic stability in cell membranes and lipoproteins, which act as endogenous NO2-FA reservoirs which can be supplied to other locations in the cell in order to act as signaling molecules [15]. An additional biological property of NO2-FAs is their capacity to mediate post-translational modifications through nitroalkylation, which will be discussed below [46,51,[66][67][68].  Release of nitric oxide from nitroalkenes through a rearrangement process. A nitrite ester is formed and homolyzed to yield NO and an enol radical (modified from Reference [15]).
Following the discovery of the presence of endogenous NO 2 -FAs and their biological properties in animal and human systems, their metabolism and distribution have been examined. In this regard, NO 2 -FAs have been shown to bind carrier proteins such as albumin, may be subjected to the normal lipid metabolism processes such as saturation and β-oxidation and can be esterified into complex lipids [22,49,69,70]. A recent study has shown that prostaglandin reductase leads to the reduction of NO 2 -FA to electrophilic nitroalkanes and that both alkenes and nitroalkanes are subjected to β-oxidation [71]. On the other hand, gastric digestion and inflammatory conditions lead to the formation of complex lipids containing NO 2 -FAs, as the formation of triglycerides (TGs) containing NO 2 -FAs has been detected in adipocytes and rat plasma following the in vitro acidic gastric digestion of TGs with NO 2 -OA supplementation [69]. Phospholipids containing NO 2 -FAs have also been uncovered in cardiac mitochondria and cardiomyoblasts from a diabetes mellitus animal model [72]. All these studies illustrate the presence of NO 2 -FAs and their metabolites in complex lipids. Lipase action can cause these NO 2 -FA-containing complex lipids to release electrophilic species. In addition, free electrophilic species may travel to remote tissues to regulate cell homeostasis and tissue signaling [49].

Nitro-Fatty Acids in Plants
Nitrate fatty acids have been widely regarded as novel mediators of cell signaling in animal organisms. However, the knowledge about them in the plant kingdom is limited. The constitutive presence of NO 2 -FAs in plant systems was initially characterized in extra-virgin olive oil (EVOO) (a basic component of the Mediterranean diet) in which oleic acid, followed by palmitic (PA), linoleic (LA), and linolenic (Ln) acids are present [73][74][75]. Given their properties mentioned above, the inherent occurrence of NO 2 -FAs in EVOO and olives was analyzed using mass spectrometry techniques. Different endogenous NO 2 -cLA isomers were detected in EVOO, while intrinsic NO 2 -OA-cysteine adducts (higher levels in the olive peel) were found in olives. These reports demonstrate that both EVOO and olives are sources and endogenous reservoirs of NO 2 -FAs, which could be responsible of the anti-inflammatory and anti-hypertensive properties of EVOO [10,70].
Additionally, the presence of NO 2 -FAs has been recently reported in both cell-suspension cultures (ACSC) and seedlings of the model plant Arabidopsis thaliana. Originally, the model plant's lipid composition was analyzed, with a predominance of Ln, followed by LA and OA [10]. The biological occurrence of NO 2 -Ln (Table 1) was only detected in ACSC (0.28 pmol/g FW) and seedlings (3.84 pmol/g FW) [9,10], while a modulation in NO 2 -Ln levels was detected during plant growth. Seeds, 14-day-old seedlings and leaves from 30-and 45-day-old Arabidopsis plants were used. The higher NO 2 -Ln content (11.18 pmol/g FW) was quantified at the seeds stage, with a continuous decline observed in the final vegetative and reproductive stages of the life cycle (0.54 pmol/g FW) [9,10]. In addition, the potential of NO 2 -Ln to emit NO has been recently evidenced [9,76], and the high NO 2 -Ln content in seeds could provide an additional source of NO which could favor germination and the onset of vegetative development [9,[77][78][79].
Mass spectrometry techniques were also used to analyze the profile of NO 2 -FAs in other plant species. In this sense, NO 2 -Ln was detected in rice (Oryza sativa) leaves (0.748 pmol/g FW). The same type of NO 2 -FA was identified in pea leaves (Pisum sativum) mitochondria (0.084 pmol/g FW) and peroxisomes (0.282 pmol/g FW) and roots (0.072 pmol/g FW). These analyses show the wide spread of NO 2 -FAs in plant organisms [10,76]. Furthermore, the levels of NO 2 -Ln detected in plants are similar to those found in animal systems [31], which reinforces their essential role as signaling contributors in plants [10,80].
On the other hand, the NO 2 -Ln abundance was also quantified in Arabidopsis under adverse environmental conditions such as mechanical wounding, salinity, low temperature, and heavy-metal stress. Under these stress situations, a meaningful rise in NO 2 -Ln content was monitored accompanied by an induction of genes associated with oxidative stress and oxygen-containing compound responses [9,80,81].
After demonstrating its relationship with plant development and plant adverse situations, a transcriptomic analysis by RNA-seq technology allowed us to analyze the signaling role played by NO 2 -Ln. Initially, ACSC treated with increasing concentrations of NO 2 -Ln (10 µM and 100 µM) showed this molecule's clear signaling response in terms of plant physiology and dose-dependence responses [9] previously described in animal systems [47,82]. A set of overexpressed genes related to abiotic and oxidative stress responses were detected after treatment with NO 2 -Ln, while other genes implicated in biological procedures, such as biosynthesis of cellular metabolites, were downregulated, with a similar pattern being observed in seedlings. It is important to highlight the involvement of upregulated genes in protein folding as well as in responses to heat and H 2 O 2 stress. Unexpectedly, around 40% of the genes which responded to NO 2 -Ln were involved in heat-shock responses (HSRs) [9]. In animal systems, the treatment with NO 2 -OA also activates a considerable number of genes related to HSRs, which reveals the presence of a conserved mechanism of response to NO 2 -FAs in both animal and plant systems [9,82].
Among the upregulated genes which responded to reactive oxygen species (ROS) is a gene encoding for cytosolic ascorbate peroxidase 2 (APX2), which is a relevant enzyme involved in defending plants against H 2 O 2 . Additionally, under abiotic stress situations such as high temperatures and light intensity, interactions between APX2 and the heat shock transcription factor (HSFA2) have been detected [10,83].
Although the participation of NO 2 -Ln in plant biology and responses to abiotic stress conditions has been previously described, the mechanisms involved in NO 2 -Ln's defense responses to stress in plants are still little known. As with animal systems, the release of NO by NO 2 -Ln in aqueous medium, which could be a signaling mechanism, has been demonstrated in Arabidopsis cell cultures by various in vitro experimental techniques such as ozone chemiluminescence, 4,5-diaminofluorescein (DAF-2) spectrofluorometric probes, confocal laser scanning microscopy, and the oxyhemoglobin oxidation method. Ozone chemiluminescence showed that NO-releasing from NO 2 -FA was not propitious in acidic locations, since at neutral pH (7.4) the maximum releasing of NO was achieved. This finding may be of considerable importance inside the cells, as mitochondria, peroxisomes, and the cytosol have a basic or neutral pH [10,76]. In addition, when the leaves and roots of Arabidopsis seedlings were treated with NO 2 -Ln, green fluorescence arose as a consequence of the increase in NO content, thus demonstrating the in vivo capability of NO 2 -Ln to provide NO. In addition, the subsequent treatment of samples with the NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) causes a decrease in fluorescence [9,84]. These results emphasize the important role of NO 2 -Ln as a NO reservoir, and thus, the indirect involvement of NO 2 -FAs in plant growth, in the response to (a)biotic stress processes and in a variety of NO-related post-translational modifications (NO-PTMs) [80,[85][86][87].

Nitroalkylation
Nitro-fatty acids, which are potent electrophiles owing to the presence of electron-withdrawing nitro (-NO 2 ) substituents in the beta carbon, mainly act via post-translational modifications. For this reason, they are able to react with nucleophiles like glutathione or target amino acid residues, which affects their protein structure and eventually their function and subcellular localization [67,88]. The nitroalkylation PTM involves the establishment of a nitro-lipid-protein adduct with the cession of a couple of electrons from the nucleophile to the electrophile (NO 2 -FA) to form a covalent bond, via a Michael adduction. This process generates lipoxidation adducts (Figure 4). Nitroalkylation provokes a chain of signaling phenomena that concludes with anti-inflammatory, anti-hypersensitive, anti-tumorigenic, cytoprotective, and antioxidant effects arbitrated by NO 2 -FAs [46,89]. Diverse studies have displayed the reversible character of nitroalkylation which enables it to act as a selective signaling pathway in stressful environments. Under these conditions, the rise in the ROS and RNS levels could affect the stability of nitroalkylation. Reactive oxygen and nitrogen species (ROS and RNS) can cause the oxidation of the bond between the sulfur residues and the α-carbon of the NO2-FAs (Michael adduct) resulting both in the generation of sulfoxides and derived species and the scission of the Michael adduct. This process results in the releasing of the nitroalkene which enables the protein to recover its initial state [22,66,81,88,90,91]. The reversible possibilities of nitroalkylation in biological processes are of considerable importance, as irreversible PTMs usually lead to permanent loss of function, and thus protein degradation [22,46,68]. Although the main nucleophiles which react with NO2-FA are cysteine thiols (Cys-SH), and not all are able to react with electrophiles, in this sense, the deprotonated cysteine thiolate (Cys-S − ) is specifically the most prone to react [92,93]. Other nucleophiles are the amino substituents of lysine and arginine residues and the imidazole moiety of histidine [89].

Nitroalkylation in Animals
Nitrate fatty acids act as signaling mediators, since a scant amount of them act as powerful signal transduction cascade mediators that carry out changes in protein function through PTMs [1,66,68,94]. As mentioned above, processes such as digestion and inflammation lead to the genesis of NO2-FAs, predominantly NO2-cLA. In animal systems, NO2-FAs protect against a broad cluster of diseases such as atherosclerosis, restenosis, ischemia-reperfusion, renal injury, diabetes, metabolic syndrome, endotoxemia, and triple-negative breast cancer [95,96]. Their pluripotent cell signaling capacity enables NO2-FAs to modulate various intracellular pathways. In this line, the capacity of NO2-FAs to release NO via the Nef reaction generates low concentrations of NO which modulates cyclic monophosphate guanosine (cGMP)-dependent cell signaling activity. Nitrate fatty acids also control the generation of NO by regulating endothelial and inducible nitric oxide synthase (eNOS and iNOS) independently of cGMP mechanisms [34,62,76].
In addition, NO2-FAs can regulate the expression levels of differentiation-related, key inflammation, and cell proliferation genes [82,97−101]. Signaling via the Kelch-like ECH-associated protein 1 (Keap 1)-nuclear factor erythroid-derived 2-like 2 (Nrf2) pathway is a primary regulator of cellular responses to oxidative stress. The transcription factor Nrf2, which controls antioxidant protein expression, is located in the cytosol in its inactive form due to Keap1 activity which promotes Nrf2 ubiquitination and subsequent degradation by the ubiquitin-proteasome system. Keap 1 contains reactive cysteines (Cys 151, 273, and 288) which can be modified by oxidation or alkylation and used as redox state sensors. When electrophiles such as NO2-OA, NO2-LA, and NO2-AA are formed, the interaction between Nrf2 and Keap1 is interrupted. This facilitates the transfer of Nrf2 to the nucleus, where it will link to specific cis targets and activate the regulation of Diverse studies have displayed the reversible character of nitroalkylation which enables it to act as a selective signaling pathway in stressful environments. Under these conditions, the rise in the ROS and RNS levels could affect the stability of nitroalkylation. Reactive oxygen and nitrogen species (ROS and RNS) can cause the oxidation of the bond between the sulfur residues and the α-carbon of the NO 2 -FAs (Michael adduct) resulting both in the generation of sulfoxides and derived species and the scission of the Michael adduct. This process results in the releasing of the nitroalkene which enables the protein to recover its initial state [22,66,81,88,90,91]. The reversible possibilities of nitroalkylation in biological processes are of considerable importance, as irreversible PTMs usually lead to permanent loss of function, and thus protein degradation [22,46,68]. Although the main nucleophiles which react with NO 2 -FA are cysteine thiols (Cys-SH), and not all are able to react with electrophiles, in this sense, the deprotonated cysteine thiolate (Cys-S − ) is specifically the most prone to react [92,93]. Other nucleophiles are the amino substituents of lysine and arginine residues and the imidazole moiety of histidine [89].

Nitroalkylation in Animals
Nitrate fatty acids act as signaling mediators, since a scant amount of them act as powerful signal transduction cascade mediators that carry out changes in protein function through PTMs [1,66,68,94]. As mentioned above, processes such as digestion and inflammation lead to the genesis of NO 2 -FAs, predominantly NO 2 -cLA. In animal systems, NO 2 -FAs protect against a broad cluster of diseases such as atherosclerosis, restenosis, ischemia-reperfusion, renal injury, diabetes, metabolic syndrome, endotoxemia, and triple-negative breast cancer [95,96]. Their pluripotent cell signaling capacity enables NO 2 -FAs to modulate various intracellular pathways. In this line, the capacity of NO 2 -FAs to release NO via the Nef reaction generates low concentrations of NO which modulates cyclic monophosphate guanosine (cGMP)-dependent cell signaling activity. Nitrate fatty acids also control the generation of NO by regulating endothelial and inducible nitric oxide synthase (eNOS and iNOS) independently of cGMP mechanisms [34,62,76].
In addition, NO 2 -FAs can regulate the expression levels of differentiation-related, key inflammation, and cell proliferation genes [82,[97][98][99][100][101]. Signaling via the Kelch-like ECH-associated protein 1 (Keap 1)-nuclear factor erythroid-derived 2-like 2 (Nrf2) pathway is a primary regulator of cellular responses to oxidative stress. The transcription factor Nrf2, which controls antioxidant protein expression, is located in the cytosol in its inactive form due to Keap1 activity which promotes Nrf2 ubiquitination and subsequent degradation by the ubiquitin-proteasome system. Keap 1 contains reactive cysteines (Cys 151, 273, and 288) which can be modified by oxidation or alkylation and used as redox state sensors. When electrophiles such as NO 2 -OA, NO 2 -LA, and NO 2 -AA are formed, the interaction between Nrf2 and Keap1 is interrupted. This facilitates the transfer of Nrf2 to the nucleus, where it will link to specific cis targets and activate the regulation of antioxidant response element (ARE) genes [1,55,97,[102][103][104][105]. The NO 2 -FA-sensitive system involving heat-shock responses (HSRs) is a complex alliance of regulatory proteins and transcription factors which promotes cytoprotective and anti-inflammatory target gene expression [46]. Heat-shock proteins (HSPs) are chaperones whose expression is triggered by stress conditions, including heat, as well as by electrophilic and reactive species caused under inflammatory injury. Chaperones prevent the aggregation of denatured or oxidized proteins, collaborate in the transfer of these proteins to intracellular locations, and thus contribute to cellular redox homeostasis [106]. Nitro-oleic acid in human endothelial cells has been reported to activate HSF1 (Heat Shock Factor 1), the most important regulator of HSRs, followed by a remarkable induction of a large group of heat shock genes (Table 2) [82,102,107].
Nitro-fatty acid can also activate the peroxisome proliferator-activating receptor (PPAR), particularly PPARγ, which is included in the family of nuclear hormone receptors. This receptor plays a marked role in the expression of transcription factors associated with lipid generation, lipid and glucose metabolism, macrophage differentiation, and immune responses. The PPARγ regulatory domain is located in the C-terminal side which coincides with the ligand binding domain. The location of a cysteine at position 285 makes this hydrophobic region susceptible to nitroalkylation by NO 2 -FAs such as NO 2 -OA and NO 2 -LA (Table 2) [1,101,[108][109][110].
Another example is the nuclear factor kappa betta (NF-kβ) involved in transcriptional regulation under inflammatory and immune processes. The nuclear factor kappa betta is a protein complex with two subunits (p50 and p65) [1,98,111,112]. Experimental studies have shown that NF-kβ is regulated by NO 2 -FAs at multiple levels including the inhibition of Toll-like receptor 4 (TLR4) by NO 2 -OA. Toll-like receptor 4 is a transmembrane protein which pertains to the pattern recognition receptor (PRR) family which is able to recognize bacterial lipopolysaccharide (LPS). Its activation triggers the intracellular NF-κB signaling pathway and inflammatory cytokine production which activate the innate immune system. Thus, the inhibition of TLR4 by NO 2 -FAs also triggers the inhibition of NF-kβ [101,113]. Another level of regulation is the inhibition of NF-kβ by nitroalkylation, specifically, the residue Cys38, placed in the DNA-binding domain of the p65 subunit, is susceptible to nitroalkylation [96,98]. The final level of regulation is the activation of PPAR by NO 2 -FAs which causes the trans-repression of inflammatory genes such as NF-kβ (Table 2) [101,114].

Nitroalkylation in Plants
Although the effects of nitroalkylation have been extensively studied in animal organisms, the impact of NO 2 -FA action in plants, which has not been fully explored, constitutes an emerging area of interesting research work. Probably, the signaling function of NO 2 -Ln is due to nitroalkylation processes. In this context, the endogenous presence of 37 proteins adducted with NO 2 -Ln in Arabidopsis cell cultures has been identified. However, cell cultures treated with 100 µM NO 2 -Ln showed an increase in the number of nitroalkylated proteins (342), belonging to different areas of cell metabolism, which included APX2 (unpublished results), whose encoding gene expression, according to the transcriptomic studies mentioned above, was induced [9].
Ascorbate peroxidase (APX2) is one of the primary antioxidant systems in plants. This enzyme belongs to the ascorbate-glutathione cycle, which detoxifies hydrogen peroxide and contains non-enzymatic antioxidants (ascorbate and glutathione) and enzymatic antioxidants such as monodehydroascorbate reductase (MDAR), glutathione reductase (GR), and dehydroascorbate reductase (DHAR), as well as the reductive coenzyme NADPH [127,128].
In this study, the APX recombinant protein from Arabidospsis thaliana was incubated with increasing concentrations of NO 2 -Ln (1 µM and 10 µM). The enzymatic activity was spectrophotometrically monitored [129]. Furthermore, the nitroalkylation targeted residues of the treated recombinant protein were detected and characterized using LC-MS/MS. Thus, the protein was digested by trypsin and desalted by C18 columns to obtain the peptide fraction which was analyzed using an Exactive Q mass spectrometer attached to a nano-flow liquid chromatograph (nanoLC) (Thermo Fisher Scientific). The LC-MS/MS spectrum deconvolution was carried out employing Proteome Discoverer version 1.4. bioinformatics software (Thermo Fisher Scientific). The Percolator node was used to filter the peptides at a 1% false discovery rate (FDR) at the peptide-spectrum matches (PSMs).
In order to identify the position of the nitroalkylation-targeted nucleophilic residues, an in silico modeling was carried out using Raptor X bioinformatics software (http://raptorx.uchicago.edu/). The APX model was based on the structure of isoniazid (INH) bound to cytosolic soybean ascorbate peroxidase (PDB:2VCF) [130].
The treatment of recombinant APX with NO 2 -Ln modulates its enzymatic activity, showing a significant decrease in the presence of 10 µM NO 2 − Ln ( Figure 5). This decreased activity was associated with the post-translational modification caused by nitroalkylation, which was detected by mass spectrometry. Comparison of the spectra of control and NO 2 -Ln-treated samples displayed a rise in the mass of nucleophilic residues due to treatment with NO 2 -Ln. The electrophilic attack by NO 2 -Ln generated the nitroalkylation of the residues showed in Figures 6 and 7A. with histidine 43 and histidine 163 being preferentially nitroalkylated. This could have functional implications ( Figure 7B), as histidine 43 and histidine 163 are located at the active and metal-binding site, respectively. This fact suggests that the nitroalkylation of these residues blocks APX enzymatic activity, modulating protein function. processes. In this context, the endogenous presence of 37 proteins adducted with NO2-Ln in Arabidopsis cell cultures has been identified. However, cell cultures treated with 100 µM NO2-Ln showed an increase in the number of nitroalkylated proteins (342), belonging to different areas of cell metabolism, which included APX2 (unpublished results), whose encoding gene expression, according to the transcriptomic studies mentioned above, was induced [9]. Ascorbate peroxidase (APX2) is one of the primary antioxidant systems in plants. This enzyme belongs to the ascorbate-glutathione cycle, which detoxifies hydrogen peroxide and contains non-enzymatic antioxidants (ascorbate and glutathione) and enzymatic antioxidants such as monodehydroascorbate reductase (MDAR), glutathione reductase (GR), and dehydroascorbate reductase (DHAR), as well as the reductive coenzyme NADPH [127,128].
In this study, the APX recombinant protein from Arabidospsis thaliana was incubated with increasing concentrations of NO2-Ln (1 µM and 10 µM). The enzymatic activity was spectrophotometrically monitored [129]. Furthermore, the nitroalkylation targeted residues of the treated recombinant protein were detected and characterized using LC-MS/MS. Thus, the protein was digested by trypsin and desalted by C18 columns to obtain the peptide fraction which was analyzed using an Exactive Q mass spectrometer attached to a nano-flow liquid chromatograph (nanoLC) (Thermo Fisher Scientific). The LC-MS/MS spectrum deconvolution was carried out employing Proteome Discoverer version 1.4. bioinformatics software (Thermo Fisher Scientific). The Percolator node was used to filter the peptides at a 1% false discovery rate (FDR) at the peptide-spectrum matches (PSMs).
In order to identify the position of the nitroalkylation-targeted nucleophilic residues, an in silico modeling was carried out using Raptor X bioinformatics software (http://raptorx.uchicago.edu/). The APX model was based on the structure of isoniazid (INH) bound to cytosolic soybean ascorbate peroxidase (PDB:2VCF) [130].
The treatment of recombinant APX with NO2-Ln modulates its enzymatic activity, showing a significant decrease in the presence of 10 µM NO2 -Ln ( Figure 5). This decreased activity was associated with the post-translational modification caused by nitroalkylation, which was detected by mass spectrometry. Comparison of the spectra of control and NO2-Ln-treated samples displayed a rise in the mass of nucleophilic residues due to treatment with NO2-Ln. The electrophilic attack by NO2-Ln generated the nitroalkylation of the residues showed in Figures 6 and 7A. with histidine 43 and histidine 163 being preferentially nitroalkylated. This could have functional implications ( Figure  7B), as histidine 43 and histidine 163 are located at the active and metal-binding site, respectively. This fact suggests that the nitroalkylation of these residues blocks APX enzymatic activity, modulating protein function. with increasing concentrations of NO2-Ln. The negative controls methanol (NO2-FA vehicle) and linolenic acid (non-nitrated fatty acid) were used. Vertical bars represent the mean ± standard deviation of at least three replicates. Statistically significant differences p < 0.05 (*) and p < 0.01 (**). (Ascorbate peroxidase: APX).     deviation of at least three replicates. Statistically significant differences p < 0.05 (*) and p < 0.01 (**). (Ascorbate peroxidase: APX).      Figure 8 explains the model of the nitro-lipid-protein adducts signaling mechanism in plants. Nitro-lipid-protein adducts stability can be affected by the accumulation of ROS and RNS, which could cause the oxidation of sulfhydryl substituents in proteins, and consequently the scission of the Michael adduct releasing NO 2 -Ln. As was previously mentioned, the nitroalkylation of APX by NO 2 -Ln generates function loss. Under nitro-oxidative conditions, the function of APX would be reactivated due to the reversibility of the nitroalkylation PTM. On the other hand, the levels of free NO 2 -FA increase, being able to stimulate the expression of heat shock proteins (HSPs) and certain antioxidant systems such as APX and methionine sulfoxide reductase B (MSRB). Another possibility is that NO 2 -FA could donate · NO in the cellular aqueous environment which could act in a broad set of plant activities such as plant development, (a)biotic disorders, antioxidant responses, and NO-PTMs. reactivated due to the reversibility of the nitroalkylation PTM. On the other hand, the levels of free NO2-FA increase, being able to stimulate the expression of heat shock proteins (HSPs) and certain antioxidant systems such as APX and methionine sulfoxide reductase B (MSRB). Another possibility is that NO2-FA could donate . NO in the cellular aqueous environment which could act in a broad set of plant activities such as plant development, (a)biotic disorders, antioxidant responses, and NO-PTMs. The ability of NO2-Ln to trigger pleiotropic signaling actions mainly depends on the nitroalkylation of regulatory proteins involved in plant biology and numerous types of (a)biotic-stress. Being a reversible post-translational modification, which can affect a large number of target amino acid residues (Cys, His, Lys, and Arg), together with the features outlined above, render nitroalkylation an important cell signaling mechanism mediated by NO2-FAs.

Conclusions and Future Perspectives
The potent electrophilic molecules NO2-FAs, whose electrophilicity triggers potential signaling mechanisms via nitroalkylation, were recently discovered in both animal and plant systems. This NO2-FA-mediated PTM can be considered a NO-PTM similar to S-nitrosylation, because NO2-FAs are RLS formed as a result of the oxidation of PUFA by NO-derived species. The importance of nitroalkylation resides in its reversibility and in the presence of a considerable amount of target amino acids residues that generate the formation of nitro-lipid-protein adducts, which enables this NO-PTM to trigger pleiotropic signaling actions. In animal systems, nitroalkylation is associated with signaling mechanisms in anti-inflammatory processes. However, in plant systems, this little-known NO-PTM constitutes an emerging area of research which should be developed through advances in mass spectrometry techniques. The ability of NO 2 -Ln to trigger pleiotropic signaling actions mainly depends on the nitroalkylation of regulatory proteins involved in plant biology and numerous types of (a)biotic-stress. Being a reversible post-translational modification, which can affect a large number of target amino acid residues (Cys, His, Lys, and Arg), together with the features outlined above, render nitroalkylation an important cell signaling mechanism mediated by NO 2 -FAs.

Conclusions and Future Perspectives
The potent electrophilic molecules NO 2 -FAs, whose electrophilicity triggers potential signaling mechanisms via nitroalkylation, were recently discovered in both animal and plant systems. This NO 2 -FA-mediated PTM can be considered a NO-PTM similar to S-nitrosylation, because NO 2 -FAs are RLS formed as a result of the oxidation of PUFA by NO-derived species. The importance of nitroalkylation resides in its reversibility and in the presence of a considerable amount of target amino acids residues that generate the formation of nitro-lipid-protein adducts, which enables this NO-PTM to trigger pleiotropic signaling actions. In animal systems, nitroalkylation is associated with signaling mechanisms in anti-inflammatory processes. However, in plant systems, this little-known NO-PTM constitutes an emerging area of research which should be developed through advances in mass spectrometry techniques.
Funding: The study was supported by an ERDF grant co-financed by the Spanish Ministry of Economy and Competitiveness (Project BIO2015-66390-P) and the Junta de Andalucía (Group BIO-286).