Phytotoxicity of Essential Oils on Selected Weeds: Potential Hazard on Food Crops

The chemical composition of winter savory, peppermint, and anise essential oils, and in vitro and in vivo phytotoxic activity against weeds (Portulaca oleracea, Lolium multiflorum, and Echinochloa crus-galli) and food crops (maize, rice, and tomato), have been studied. Sixty-four compounds accounting for between 97.67–99.66% of the total essential oils were identified by Gas Chromatography-Mass Spectrometry analysis. Winter savory with carvacrol (43.34%) and thymol (23.20%) as the main compounds produced a total inhibitory effect against the seed germination of tested weed. Menthol (48.23%), menthone (23.33%), and iso-menthone (16.33%) from peppermint only showed total seed germination inhibition on L. multiflorum, whereas no significant effects were observed with trans-anethole (99.46%) from anise at all concentrations (0.125–1 µL/mL). Low doses of peppermint essential oil could be used as a sustainable alternative to synthetic agrochemicals to control L. multiflorum. The results corroborate that in vivo assays with a commercial emulsifiable concentrate need higher doses of the essential oils to reproduce previous in vitro trials. The higher in vivo phytotoxicity of winter savory essential oil constitutes an eco-friendly and less pernicious alternative to weed control. It is possible to achieve a greater in vivo phytotoxicity if less active essential oil like peppermint is included with other active excipients.


Introduction
The potential hazard to the environment and human health, as well as the emergence of resistant weeds, are still the main problems of the overuse of synthetic herbicides used to improve global crop productivity. The continuous use of glyphosate, marketed in 1974 as a highly effective broad-spectrum herbicide [1], has made particular populations, such as the annual ryegrass (Lolium rigidum L.) in Australia [2] or barnyardgrass (Echinochloa crus-galli (L.) Beauv.) in cotton fields of the midsouthern United States [3], become glyphosate-resistant [4]. Recently, glyphosate resistance has been described in many world-wide species, like common ragweed (Ambrosia artemisiifolia L.) in several row crops of the south-eastern USA, following other still unknown mechanisms of action [5]. Together, resistant problems; potential health risks including skin irritancy, muscle atrophy, and nerve axons damage with prolonged exposure; and the use of a surfactant to enhance penetration [6] can even induce acute poisoning in humans, as well as chronic and sub-chronic toxicity, which has been reported in mammals after the consumption of contaminated food [7]. As these problems have appeared, some other weed killers have been synthesised as alternative solutions. However, the latest generation of the synthetic triketone herbicide family (sulcotrione, mesotrione, and tembotrione) also has negative impacts in microbial edaphic communities or plants, and even their degradation products can be more toxic than those of the parent, leading to similar environmental problems to glyphosate [8].

Chemical Composition of Winter Savory, Peppermint, and Anise Essential Oils
Sixty-four compounds accounting for 97.67-99.66% of the total commercial winter savory, peppermint, and anise essential oils were identified by GC/MS analysis. Components are clustered (Table 1) in homologous series of monoterpene hydrocarbons, oxygenated monoterpenes, sesquiterpene hydrocarbons, oxygenated sesquiterpenes, diterpene hydrocarbons, aromatic compounds, and others, and are listed according to Kovat's retention index calculated in GC on an apolar HP-5MS column.

Seed
Germination and Seedling Growth Inhibition of P. oleracea, L. multiflorum, and E. crus-galli, and Maize, Rice, and Tomato with Essential Oils The phytotoxic effect of winter savory, peppermint, and anise essential oils was evaluated in vitro against three known harmful herbs: P. oleracea, L. multiflorum, and E. crus-galli. In this set of trials, the high phytotoxicity of winter savory essential oil ( Table 2) was highly remarkable, exhibiting a total inhibitory effect against the seed germination of the tested weeds at all doses (0.125, 0.25, 0.50, and 1 µL/mL) assayed.
Furthermore, it was also noteworthy that the complete inhibition of the seed germination of L. multiflorum by peppermint essential oil was exhibited at all doses (0.125, 0.25, 0.50, and 1 µL/mL) applied (Table 2). Besides, significant differences between the control and the highest dose (1 µL/mL) of peppermint essential oil tested were found in the seed germination of both P. oleracea and E. crus-galli, although no significant effect at lower doses on the seed germination of P. oleracea and E. crus-galli was observed ( Table 2).
In addition, a stronger phytotoxic effect was found with peppermint essential oil against the seedling growth (hypocotyl and radicle) of P. oleracea and E. crus-galli, so it could be employed as a potential post-harvest treatment. According to P. oleracea seedling growth, significant differences were found between the control and the higher doses (0.50 and 1 µL/mL) tested (Table 3, Figure 1). Lower doses of peppermint essential oil (0.125 and 0.25 µL/mL) assayed did not cause a significant reduction in hypocotyl growth of P. oleracea seeds (37.25%), whereas a moderate (50.98%) inhibitory effect was observed when the highest dose (1 µL/mL) was applied. A similar result was found in radicle elongation, with a percentage inhibition of 43.48% at the two higher concentrations (Table 3). E. crus-galli seedling growth was more sensible to peppermint essential oil, experiencing a significant reduction in both hypocotyl and radicle development with respect to the control (Table 3, Figure 1b). There was no major difference in either hypocotyl or radicle enlargement when comparing    Anise essential oil showed the absence of a significant herbicidal effect for both the doses and weed species tried ( Table 2). It displayed no significant phytotoxic activity against seed germination ( Table 2) of weeds affecting food crops; however, the seedling growth (hypocotyl and radicle) of L. multiflorum was significantly inhibited in a dose-dependent manner (Table 4), as well as the hypocotyl development of E. crus-galli that was depleted (63.23%) at the highest (1 µL/mL) dose and the radicle elongation at all concentrations applied with a percentage inhibition between 36.29 to 65.40% (Table  4, Figure 2c).  Anise essential oil showed the absence of a significant herbicidal effect for both the doses and weed species tried (Table 2). It displayed no significant phytotoxic activity against seed germination (Table 2) of weeds affecting food crops; however, the seedling growth (hypocotyl and radicle) of L. multiflorum was significantly inhibited in a dose-dependent manner (Table 4), as well as the hypocotyl development of E. crus-galli that was depleted (63.23%) at the highest (1 µL/mL) dose and the radicle elongation at all concentrations applied with a percentage inhibition between 36.29 to 65.40% (Table 4, Figure 2c).  The previous selective inhibitory effect at the concentrations applied displayed by peppermint essential oil against the three assayed weeds with a total L. multiflorum seed germination inhibition, was not observed against the three selected crops (Table 5). Tomato was the most sensible crop, with an almost complete seed germination inhibition (96.84%) at the highest dose assayed (1 µL/mL), followed by maize (79.31%) and rice (36.96%). Peppermint essential oil significantly affected the growth of both the hypocotyl and radicle of rice and tomato ( Table 5). The maize radicle was also significantly disturbed by these treatments in comparison to the control, whereas the volatile oil did not affect the hypocotyl growth of maize. It is interesting to note the results of peppermint essential oil against rice and L. multiflorum, which show a lower phytotoxic effect in rice, with a seed germination inhibition percentage between 18.48% to 16.30% at the doses of 0.125, 0.25, and 0.50 µL/mL ( Table 5); concentrations that cause a total seed germination inhibition of L. multiflorum, one of the principal weeds that affect this crop ( Table 2). 3.00 ± 3.00 c 0.20 ± 0.20 b 1.12 ± 1.12 b The previous selective inhibitory effect at the concentrations applied displayed by peppermint essential oil against the three assayed weeds with a total L. multiflorum seed germination inhibition, was not observed against the three selected crops (Table 5). Tomato was the most sensible crop, with an almost complete seed germination inhibition (96.84%) at the highest dose assayed (1 µL/mL), followed by maize (79.31%) and rice (36.96%). Peppermint essential oil significantly affected the growth of both the hypocotyl and radicle of rice and tomato ( Table 5). The maize radicle was also significantly disturbed by these treatments in comparison to the control, whereas the volatile oil did not affect the hypocotyl growth of maize. It is interesting to note the results of peppermint essential oil against rice and L. multiflorum, which show a lower phytotoxic effect in rice, with a seed germination inhibition percentage between 18.48% to 16.30% at the doses of 0.125, 0.25, and 0.50 µL/mL ( Table 5); concentrations that cause a total seed germination inhibition of L. multiflorum, one of the principal weeds that affect this crop (Table 2). Table 5. In vitro seed germination and hypocotyl and radicle growth of maize, rice, and tomato seeds with peppermint essential oil.

Peppermint
Seed Germination (%) ± e.d. Seedling Growth (mm ± e.d.) The phytotoxic effect exhibited by winter savory and peppermint essential oils in in vitro trials were corroborated by in vivo conditions using two different emulsifiable concentrates elaborated by SEIPASA, a pioneer Spanish Company in the development, manufacturing, and marketing of environmentally friendly agro-inputs in order to produce healthy food.

Concentration (µL/mL) Maize
The results of the emulsifiable concentrate containing a final dose of 5 or 10 µL/mL of essential oil were compared with a control watered with water and a blank without the corresponding essential oils.
The emulsifiable concentrate of winter savory succeeded in inhibiting the seed germination of P. oleracea within 33 days at both concentrations, corroborating the previous in vitro results in which there was also total inhibition of the weed germination of common purslane ( Table 2).
Despite L. multiflorum and E. crus-galli being more tolerant species in in vivo conditions, the emulsifiable concentrate of winter savory also significantly inhibited their seed germination, with percentages of 95-100% and 82-99% at 5 and 10 µL/mL, and without a significant effect with the blank (Figure 3). Similar results were found for hypocotyl growth (Figure 4), making the formulate with winter savory essential oil an ecological alternative to synthetic herbicides, which have already demonstrated a detrimental influence on the environment, crops, and human health.
Regarding peppermint essential oil, due to the less phytotoxical effect, it was emulsified with other herbicidal compounds (blank use by SEIPASA Company) and the formulate was able to inhibit the seed germination of the three weeds in a dose-dependent manner, with percentages between 77-100% for P. oleracea, 90-95% against E. crus-galli, and total inhibition over L. multiflorum, corroborating the in vitro results in which peppermint essential oil was more active against L. multiflorum than P. oleracea and E. crus-galli. The phytotoxic effect exhibited by winter savory and peppermint essential oils in in vitro trials were corroborated by in vivo conditions using two different emulsifiable concentrates elaborated by SEIPASA, a pioneer Spanish Company in the development, manufacturing, and marketing of environmentally friendly agro-inputs in order to produce healthy food.
The results of the emulsifiable concentrate containing a final dose of 5 or 10 µL/mL of essential oil were compared with a control watered with water and a blank without the corresponding essential oils.
The emulsifiable concentrate of winter savory succeeded in inhibiting the seed germination of P. oleracea within 33 days at both concentrations, corroborating the previous in vitro results in which there was also total inhibition of the weed germination of common purslane ( Table 2).
Despite L. multiflorum and E. crus-galli being more tolerant species in in vivo conditions, the emulsifiable concentrate of winter savory also significantly inhibited their seed germination, with percentages of 95-100% and 82-99% at 5 and 10 µL/mL, and without a significant effect with the blank (Figure 3). Similar results were found for hypocotyl growth (Figure 4), making the formulate with winter savory essential oil an ecological alternative to synthetic herbicides, which have already demonstrated a detrimental influence on the environment, crops, and human health.   Values are mean of five replications ± error deviation after 14 days of incubation. Means followed by different letters in the same column indicate that they are significantly different at p < 0.05 according to T3 Dunnet and Tukey tests.

Seed Germination and Seedling Growth Inhibition of P. oleracea, L. multiflorum, and E. crus-galli, and Maize, Rice, and Tomato with an Emulsifiable Concentrate Including Winter Savory or Peppermint Essential Oils
The phytotoxic effect exhibited by winter savory and peppermint essential oils in in vitro trials were corroborated by in vivo conditions using two different emulsifiable concentrates elaborated by SEIPASA, a pioneer Spanish Company in the development, manufacturing, and marketing of environmentally friendly agro-inputs in order to produce healthy food.
The results of the emulsifiable concentrate containing a final dose of 5 or 10 µL/mL of essential oil were compared with a control watered with water and a blank without the corresponding essential oils.
The emulsifiable concentrate of winter savory succeeded in inhibiting the seed germination of P. oleracea within 33 days at both concentrations, corroborating the previous in vitro results in which there was also total inhibition of the weed germination of common purslane ( Table 2).
Despite L. multiflorum and E. crus-galli being more tolerant species in in vivo conditions, the emulsifiable concentrate of winter savory also significantly inhibited their seed germination, with percentages of 95-100% and 82-99% at 5 and 10 µL/mL, and without a significant effect with the blank (Figure 3). Similar results were found for hypocotyl growth (Figure 4), making the formulate with winter savory essential oil an ecological alternative to synthetic herbicides, which have already demonstrated a detrimental influence on the environment, crops, and human health.   According to the herbicidal effect of the emulsifiable concentrate of winter savory, a new set of trials was carried out with this formulate and three food crops. Unfortunately, a total seed germination inhibition was obtained with maize and rice, and between 80 and 98% of the tomato was inhibited at 5 and 10 µL/mL, respectively, with no significant effect with the blank (Figure 5).
Regarding peppermint essential oil, due to the less phytotoxical effect, it was emulsified with other herbicidal compounds (blank use by SEIPASA Company) and the formulate was able to inhibit the seed germination of the three weeds in a dose-dependent manner, with percentages between 77-100% for P. oleracea, 90-95% against E. crus-galli, and total inhibition over L. multiflorum, corroborating the in vitro results in which peppermint essential oil was more active against L. multiflorum than P. oleracea and E. crus-galli.
According to the herbicidal effect of the emulsifiable concentrate of winter savory, a new set of trials was carried out with this formulate and three food crops. Unfortunately, a total seed germination inhibition was obtained with maize and rice, and between 80 and 98% of the tomato was inhibited at 5 and 10 µL/mL, respectively, with no significant effect with the blank (Figure 5). Figure 5. In vivo effect of the emulsifiable concentrate of winter savory essential oil over the germination and hypocotyl growth of tomato seeds. Values are mean of ten replications ± error deviation after 30 days of incubation. Means followed by different letters in the same column indicate that they are significantly different at p < 0.05 according to T3 Dunnet and Tukey tests.

Discussion
The phenolic compounds carvacrol (43.34 ± 0.09%) and thymol (23.20 ± 0.06%) were the main compounds identified in the winter savory essential oil analysed here. It is well-known that the strong spicy flavour of winter savory is determined by the prevailing carvacrol/thymol chemotype [33], which is variable in relation to the stage of development of the plant, harvesting time, and field environment conditions, including circumstances such as a variation in altitude: in fact, a higher content of linalool and other compounds found here in lower quantities (Table 1), such as terpinen-4-ol (1.04 ± 0.01%) and sabinene hydrate (0.20 ± 0.01%), has been detected in higher amounts in S. montana essential oil at a higher altitude, while both the major ones identified here, carvacrol and thymol, were quantified in lower percentages [34].
Apart from that, these phenolic compounds found in winter savory essential oil are considered the main bioactive monoterpenes that provide S. montana with a wide range of pharmacological and biological properties, such as natural antimicrobial activity [35] against gram-positive (Staphylococcus aureus and Bacillus cereus) and gram-negative bacteria (Salmonella infantis and Escherichia coli O157:H7) [36] that is useful in the treatment of foodborne diseases, as well as anti-inflammatory activity for certain transcription factors [37]. Furthermore, recently, some new thymol and carvacrol derivatives, including the carbamate moiety, have been synthesized with stronger inhibitory effects on acetylcholinesterase [38]. Related to their phytotoxicity, both carvacrol and thymol have shown total suppression of the seed germination and seedling growth of Amaranthus retroflexus, Chenopodium album, and Rumex crispus [39], coinciding with our authors [27] and also with previous [40] studies, in which oregano essential oil with 60.42% of carvacrol exhibited a total inhibition of P. oleracea, L. multiflorum, and E. crus-galli at the same in vitro doses assayed. These results indicate that winter savory is an effective broad-spectrum herbicide as it occurs with glyphosate that also exerts inhibitory effects on the seed germination of crops, such as wheat (Triticum durum L.), pea (Pisum sativum L.), lettuce (Latuca sativa L.) [41], and even trees, for instance Pinus pinaster, in which it is able to induce Figure 5. In vivo effect of the emulsifiable concentrate of winter savory essential oil over the germination and hypocotyl growth of tomato seeds. Values are mean of ten replications ± error deviation after 30 days of incubation. Means followed by different letters in the same column indicate that they are significantly different at p < 0.05 according to T3 Dunnet and Tukey tests.

Discussion
The phenolic compounds carvacrol (43.34 ± 0.09%) and thymol (23.20 ± 0.06%) were the main compounds identified in the winter savory essential oil analysed here. It is well-known that the strong spicy flavour of winter savory is determined by the prevailing carvacrol/thymol chemotype [33], which is variable in relation to the stage of development of the plant, harvesting time, and field environment conditions, including circumstances such as a variation in altitude: in fact, a higher content of linalool and other compounds found here in lower quantities (Table 1), such as terpinen-4-ol (1.04 ± 0.01%) and sabinene hydrate (0.20 ± 0.01%), has been detected in higher amounts in S. montana essential oil at a higher altitude, while both the major ones identified here, carvacrol and thymol, were quantified in lower percentages [34].
Apart from that, these phenolic compounds found in winter savory essential oil are considered the main bioactive monoterpenes that provide S. montana with a wide range of pharmacological and biological properties, such as natural antimicrobial activity [35] against gram-positive (Staphylococcus aureus and Bacillus cereus) and gram-negative bacteria (Salmonella infantis and Escherichia coli O157:H7) [36] that is useful in the treatment of foodborne diseases, as well as anti-inflammatory activity for certain transcription factors [37]. Furthermore, recently, some new thymol and carvacrol derivatives, including the carbamate moiety, have been synthesized with stronger inhibitory effects on acetylcholinesterase [38]. Related to their phytotoxicity, both carvacrol and thymol have shown total suppression of the seed germination and seedling growth of Amaranthus retroflexus, Chenopodium album, and Rumex crispus [39], coinciding with our authors [27] and also with previous [40] studies, in which oregano essential oil with 60.42% of carvacrol exhibited a total inhibition of P. oleracea, L. multiflorum, and E. crus-galli at the same in vitro doses assayed. These results indicate that winter savory is an effective broad-spectrum herbicide as it occurs with glyphosate that also exerts inhibitory effects on the seed germination of crops, such as wheat (Triticum durum L.), pea (Pisum sativum L.), lettuce (Latuca sativa L.) [41], and even trees, for instance Pinus pinaster, in which it is able to induce in vitro shoot chlorosis and drooping [42]. So, this formulate could be an alternative to glyphosate with less environmental and human health problems.
Several researches corroborate that the biological properties of peppermint essential oil are due to its chemical composition, especially its major components menthol, menthone, and iso-menthone [43,44], conferring the high percentage of menthol to peppermint essential oil immunostimulant effects in animals [45] and the herbicidal effect in Mediterranean weed, such as Amaranthus retroflexus L., Solanum nigrum L., and P. oleracea under controlled conditions [46]. The results obtained convert peppermint essential oil into a sustainable alternative that can solve the recent L. multiflorum resistance to glyphosate on rice paddy leaves [47]. Not only do our results match with other studies in reference to larger compounds, but also in reference to smaller ones, such as E-nerolidol, spathulenol, and caryophyllene oxide, which were the only oxygenated sesquiterpenes identified in peppermint essential oil [48,49].
Finally, although trans-anethole, the main compound in commercial anise essential oil (99.46 ± 0.05%) analysed here and in other previous works [50] has demonstrated strong antifungal activity through the inhibition of the mycelial growth of a wide range of fungi [51] and could be used as a preservative in food preparation and processing [49], our results together with other research [52] revealed no significant phytotoxic activity against seed germination of selected weed.
With the purpose of decreasing volatility, winter savory and peppermint essential oils were included in an emulsifiable concentrate industrially prepared by Seipasa Company that was stable at room temperature and adequate for in vivo weed control assays.
Mature seeds of 'Perseo-type' maize (Zea mays L.) and 'Albufera-type' rice (Oryza sativa L.) were obtained from the cereals in Sueca (Valencia, Spain). 'Huevo de toro-type' tomato (Solanum lycopersicum L.) seeds were directly acquired from the fruit found in the inner part in Utiel (Valencia, Spain).

Gas Chromatography-Mass Spectrometry
GC-MS analysis was carried out with a 5973N Agilent apparatus, equipped with a capillary column (95 dimethylpolysiloxane-5% diphenyl), Agilent HP-5MS UI (30 m long and 0.25 mm i.d. with 0.25 µm film thickness). The column temperature program was 60 • C for 5 min, with 3 • C/min increases to 180 • C, and then 20 • C/min increases to 280 • C, which was maintained for 10 min. The carrier gas was Helium at a flow-rate of 1 mL/min. Split mode injection (ratio 1:30) was employed. Mass spectra were taken over the m/z 30-500 range with an ionizing voltage of 70 eV.

Identification
The individual compounds were identified by MS and their identity was confirmed by a comparison with their Kovat's retention index calculated using co-chromatographed standard hydrocarbons relative to C8-C32 n-alkanes, and mass spectra with reference samples or with data already available in the NIST 2005 mass spectral library and in the literature [53]. Sets of 20 seeds (10 for maize), each with five replicates (ten replicate in maize) per treatment, were homogenously distributed in Petri dishes (9 cm diameter) between two layers of filter paper (Whatman No.1) moistened with 4 mL of distilled water and with 0 (control), 0.125, 0.250, 0.5, and 1 µL/mL of winter savory, peppermint, and anise essential oils. Petri dishes were sealed with parafilm and incubated in a germination chamber Equitec EGCS 301 3SHR model, according to previous assays [54], alternating between 30.0 ± 0.1 • C 16 h in light and 20.0 ± 0.1 • C 8 h in dark, with (E. crus-galli, maize, and rice) and without (P.oleracea, L. multiflorum, and tomato) humidity. To evaluate the herbicidal activity of the essential oils, the number of germinated seeds was counted and compared with those of untreated seedlings. Emergence of the radicle (≥1 mm) was used as an index of germination and seedling length (hypocotyl and/or radicle) data were recorded after 3, 5, 7, 10, and 14 days in each replicate. 4.6. In Vivo Assays: P. oleracea, L. multiflorum, E. crus-galli, Maize, Rice, and Tomato with an Emulsifiable Concentrate of Winter Savory or Peppermint Ten seeds of each species (P. oleracea, L. multiflorum, E. crus-galli, maize, rice, and tomato) with ten replicates per treatment were randomly chosen and placed in pods (9 cm diameter) with 40 g of substrate. They were place less than 1 cm below Substrate Projar Professional containing coir and peat make, fertilizer N-P-K: 14 + 16 + 18 + micronutrients, and dolomitic limestone with a sorption capacity of 183 g/10 min. A set of 10 pods was watered on the first day with 20 mL of water (control), 20 mL commercial products (Nosbur OE 12 NS (32% w/w), Emulson AG/CAL/E (7% w/w), Emulson CO 36 (13% w/w) or Emulson AG/CAL/E (2.2% w/w), Alpicare 410H (21.7% w/w), Emulson AG/7720/A (2.6% w/w) respectively) without essential oils (blank), and 20 mL of emulsifiable concentrate with winter savory (48% w/w) or peppermint essential oil (73.5 % w/w) at 5 and 10 µL/mL. A tray was used every five pods to hold and separate them when watering. In order to prevent leaching, the pods were covered with plastic film. Over a period of 33 or 20 days (winter savory or peppermint), each tray was watered with 250 mL of water every two days. The greenhouse conditions were: 23.3 • C average indoor temperature, 18.1 • C minimum indoor temperature, 29.7 • C maximum indoor temperature, 57.2% average humidity, 80.9 µmol/m 2 /s PAR (Photo Active Radiation), and 135.6 W/m 2 intensity of radiation.
To evaluate the herbicidal effect, the number of germinated seeds in 5 µL /mL and 10 µL /mL pod trays was counted and compared with those of control and blank samples. Emergence of the hypocotyl (≥1 mm) was used as an index of germination and seedling length data were recorded every two days, coinciding with watering days over 33 or 20 days.

Statistical Analysis
Experiments were conducted with five replicates and ten replicates in vitro and in vivo, respectively. Data were subjected to one-way analysis of variance (ANOVA) with SPSS statistics 22 software. Tukey's post hoc test was used when variances remained homogeneous (Levene's test) and T3 Dunnett's post hoc one was employed if not, assuming equal variances. Differences were considered to be significant at p ≤ 0.05.

Conclusions
The results in vitro showed that winter savory and peppermint essential oils can be effective bioherbicides. Peppermint essential oil at lower doses could be used to control L. multiflorum in rice. The emulsifiable concentrate based on winter savory essential oil tested in in vivo assays corroborates that this effective broad-spectrum herbicide constitutes an eco-friendly and less pernicious alternative to glyphosate in weed control.