Deciphering Winter Sprouting Potential of Erianthus procerus Derived Sugarcane Hybrids under Subtropical Climates

Winter sprouting potential and red rot resistance are two key parameters for successful sugarcane breeding in the subtropics. However, the cultivated sugarcane hybrids had a narrow genetic base; hence, the present study was planned to evaluate the Erianthus procerus genome introgressed Saccharum hybrids for their ratooning potential under subtropical climates and red rot tolerance under tropical and subtropical climates. A set of 15 Erianthus procerus derived hybrids confirmed through the 5S rDNA marker, along with five check varieties, were evaluated for agro-morphological, quality, and physiological traits for two years (2018–2019 and 2019–2020) and winter sprouting potential for three years (2018–2019, 2019–2020, and 2020–2021). The experimental material was also tested against the most prevalent isolates of the red rot pathogen in tropical (Cf671 and Cf671 + Cf9401) and subtropical regions (Cf08 and Cf09). The E. procerus hybrid GU 12—19 had the highest winter sprouting potential, with a winter sprouting index (WSI) of 10.6, followed by GU 12—22 with a WSI of 8.5. The other top-performing hybrids were as follows: GU 12—21 and GU 12—29 with a WSI of 7.2 and 6.9, respectively. A set of nine E. procerus-derived hybrids, i.e., GU04 (28) EO—2, GU12—19, GU12—21, GU12—22, GU12—23, GU12—26, GU12—27, GU12—30, and GU12—31, were resistant to the most prevalent isolates of red rot in both tropical and subtropical conditions. The association analysis revealed significant correlations between the various traits, particularly the fibre content, with a maximum number of associations, which indicates its multifaceted impact on sugarcane characteristics. Principal component analysis (PCA) summarised the data, explaining 57.6% of the total variation for the measured traits and genotypes, providing valuable insights into the performance and characteristics of the Erianthus procerus derived hybrids under subtropical climates. The anthocyanin content of Erianthus procerus hybrids was better than the check varieties, ranging from 0.123 to 0.179 (2018–2019) and 0.111 to 0.172 (2019–2020); anthocyanin plays a vital role in mitigating cold injury, acting as an antioxidant in cool weather conditions, particularly in sugarcane. Seven hybrids recorded a more than 22% fibre threshold, indicating their industrial potential. These hybrids could serve as potential donors for cold tolerance and a high ratooning ability, along with red rot resistance, under subtropical climates.


Introduction
Sugarcane, a perennial crop in the genus Saccharum, has served as a key feedstock for sugar production across the globe [1].In light of climate change, it is becoming increasingly important to replace fossil fuels with sustainable bioenergy sources such as The present study was planned to evaluate the Erianthus procerus genome-introgressed Saccharum hybrids for their ratooning potential under subtropical climates and red rot tolerance under tropical and subtropical climates.Also, to study the variation in the Erianthus procerus genome-derived hybrids for agro-morphological, quality, and physiological parameters.These intergeneric hybrids could serve as valuable sources for the further introgression of high ratooning potential as well as biotic and abiotic stress tolerance into the cultivated sugarcane.

Weather Statistics of the Experimental Site
The maximum and minimum temperatures, evaporation rates, relative humidity, and rainfall pa erns of subtropical India from January 2018 to December 2020 are presented in Figure 1.In all three years, the pa ern of maximum and minimum temperatures was generally found to be similar.The monthly maximum and minimum temperature ranged between 25-45 °C and 10-30 °C, respectively.However, during peak winter (the second fortnight of December to the first fortnight of January), the minimum temperatures ranged from 4 to 8 °C.The rainfall pa ern suggests that the July-August months were the we est months in all the years; however, the distribution varied among the years.Active rainfall months were June-September in the year 2018 and June-August in the year 2020; however, in the year 2019, only July-August were the active rainfall months.In subtropical conditions, the sunshine hours also varied to a great extent, ranging from as low as 3.4 h/day (December 2019) to as high as 10.8 h/day (May 2018), which exactly corresponds to the temperature pa erns and inverse relationship with the relative humidity pa ern.

Significance of Variation Sources
The pooled ANOVA was carried out after conducting the homogeneity of residual variance test to divide the total variation in the traits into its components, and the results are presented in Table 1.The ANOVA revealed that the first main effect, i.e., the genotype effect, was highly significant (p < 0.01) for all the agro-morphologies (60-day tillers, 90-day tillers, 120-day tillers, plant height, cane diameter, and cane yield) except germination and

Significance of Variation Sources
The pooled ANOVA was carried out after conducting the homogeneity of residual variance test to divide the total variation in the traits into its components, and the results are presented in Table 1.The ANOVA revealed that the first main effect, i.e., the genotype effect, was highly significant (p < 0.01) for all the agro-morphologies (60-day tillers, 90-day tillers, 120-day tillers, plant height, cane diameter, and cane yield) except germination and the number of millable canes, which were significant at p < 0.05.Similarly, quality trait (juice weight, juice extraction, Brix value, pol %, purity, single cane weight, and fibre content) parameters were highly significant at p < 0.01 (Table 1).However, out of the five physiological parameters, only two parameters, i.e., the nitrogen balance index and chlorophyll content, exhibited a highly significant (p < 0.01) genotypic effect, whereas the remaining three parameters, i.e., the flavonoid index, anthocyanin index, and leaf area, did not exhibit significant differences among the studied traits.The other main effect, i.e., the year effect, was highly significant (p < 0.01) for germination, 90-day tillers and 120-day tillers, and significant (p < 0.05) for the pol% and fibre content, and the remaining 14 traits exhibited no significant differences among the studied genotypes.The interaction component (genotype × year) effect was highly significant (p < 0.05) only for the 90-day tillers, 120-day tillers, and fibre content, whereas the remaining 17 studied traits did not exhibit significant differences among the genotypes.The mean performance of agro-morphological, quality, and physiological parameters is illustrated as box plots in Figures 2-4 and presented in the Supplementary Tables.Although all the studied traits were influenced by the environment, the magnitude of the environmental effect was higher for the agro-morphological traits compared to the quality and physiological traits.The magnitude of variation for the number of tillers at 60 days was more than that of 90 days and 120 days.The mean germination was also highly influenced by the environmental effect.Generally, the crop year 2018-2019 was more favourable for all the studied traits, except for the germination and flavonoid index, than the crop year 2019-2020, as the trait's performance measured by the percent superiority ranged from 1.8 to 54.7.The extreme genotypes were observed for a few traits, including juice extraction, purity, fibre content, cane diameter, and cane yield, as some of the genotypes were located outside the error bars (Figures 2-4).
enced by the environmental effect.Generally, the crop year 2018-2019 was more favoura ble for all the studied traits, except for the germination and flavonoid index, than the cro year 2019-2020, as the trait's performance measured by the percent superiority range from 1.8 to 54.7.The extreme genotypes were observed for a few traits, including juic extraction, purity, fibre content, cane diameter, and cane yield, as some of the genotype were located outside the error bars (Figures 2-4).derived hybrids and check varieties.Ger: germination (%); NBI: nitrogen balance index; 60-day ti ers ('000/ha); 90-day tillers ('000/ha); 120-day tillers ('000/ha); PH: plant height (cm); CD: cane diam eter (cm); NMCs: number of millable canes ('000/ha); leaf area (cm 2 ), CHLORO: chlorophyll conce tration (µg/cm 2 ); CY: cane yield (t/ha).In the year 2018-2019, the plant height varied from 136.67 cm to 263.48 cm with a mean of 136.67 cm, and many procerus-derived hybrids were taller than the best modern cultivar, Co 0238 (Supplementary Tables).The cane diameter ranged from 1.50 cm to 2.97 cm with a mean of 2.01 cm; unlike the cane weight, the cane diameter reduced in many of the procerus-derived hybrids.The tillers at 60 days after planting and 90 days after planting were high in GU 12-30, with a mean of 131.95 and 138 '000/ha, respectively.In the year 2019-2020, the plant height varied between 120.12 and 263.33 cm with a mean of 200.73 cm, and the cane diameter varied from 1.08 cm to 2.84 cm with a mean of 1.92 cm.The tillers at 60 days after planting and 90 days after planting varied between 50.37 and 200, and 54.81 and 104.07, with mean tiller numbers of 122.63 and 77.29 '000/ha, respectively.Out of these, five entries, namely, GU12-29, GU12-28, GU12-30, GU12-34, and Plants 2024, 13, 1023 6 of 20 GU12-38, had superior performance compared to the best standard, Co 0238 (150 '000/ha), and the other five entries, namely, GU12-21, GU12-23, GU12-22, GU12-26, and GU12-27, were recorded at par value with the check Co 0238.The cane yield in the years 2018-2019 and 2019-2020 ranged from 35.15 to 99.16 t/ha and 38.12 to 92.85 t/ha, respectively, with the mean yields of 69.22 and 64.32 t/ha.The juice pol percent varied from 9.14 to 18.21 percent in 2018-2019 and from 11.36 to 16.83 percent in 2019-2020 with a mean of 14.64 and 13.96 percent, respectively.Similarly, the mean leaf area during the year 2018-2019 was 243.06 cm 2 , it was 237.19 cm 2 during 2019-2020, and Co 0238 was best among the standards with a 241 cm 2 leaf area.In the year 2018-2019, the plant height varied from 136.67 cm to 263.48 cm with a mean of 136.67 cm, and many procerus-derived hybrids were taller than the best modern cultivar, Co 0238 (Supplementary Tables).The cane diameter ranged from 1.50 cm to 2.97 cm with a mean of 2.01 cm; unlike the cane weight, the cane diameter reduced in many of the procerus-derived hybrids.The tillers at 60 days after planting and 90 days after planting were high in GU 12-30, with a mean of 131.95 and 138 '000/ha, respectively.In the year 2019-2020, the plant height varied between 120.12 and 263.33 cm with a mean of 200.73 cm, and the cane diameter varied from 1.08 cm to 2.84 cm with a mean of 1.92 cm.The tillers at 60 days after planting and 90 days after planting varied between 50.37 and 200, and 54.81 and 104.07, with mean tiller numbers of 122.63 and 77.29 '000/ha, respectively.Out of these, five entries, namely, GU12-29, GU12-28, GU12-30, GU12-34, and GU12-38, had superior performance compared to the best standard, Co 0238 (150 '000/ha), and the other five entries, namely, GU12-21, GU12-23, GU12-22, GU12-26, and GU12-27, were recorded at par value with the check Co 0238.The cane yield in the years 2018-2019 and 2019-2020 ranged from 35.15 to 99.16 t/ha and 38.12 to 92.85 t/ha, respectively, with the mean yields of 69.22 and 64.32 t/ha.The juice pol percent varied from 9.14 to 18.21 percent in 2018-2019 and from 11.36 to 16.83 percent in 2019-2020 with a mean of 14.64 and 13.96 percent, respectively.Similarly, the mean leaf area during the year 2018-2019 was 243.06 cm 2 , it was 237.19 cm 2 during 2019-2020, and Co 0238 was best among the standards with a 241 cm 2 leaf area.

Flavonoid and Anthicyanin Indices
The flavonoid index ranged from 0.89 to 1.38 (2018-2019) and 0.93 to 2.16 (2019-2020), whereas the mean flavonoid index of 1.14 and 1.23 was recorded, respectively, during the

Correlation Studies
The correlation coefficients of the agro-morphological, quality, and physiological traits are given in Table 2.The cane yield had a significant positive association with the number of tillers at 60 days (0.57) and 90 days (0.39), the plant height (0.51), the single cane weight (0.84), the purity (0.35), and the fibre content (0.35); however, the juice weight had a significant negative association.The important quality trait, i.e., pol%, had a significant positive correlation with the cane diameter, juice weight, juice extraction, Brix value, purity, and single cane weight, and a significant negative association with the fibre content, number of millable canes, and nitrogen balance index.The fibre content had a significant

Correlation Studies
The correlation coefficients of the agro-morphological, quality, and physiological traits are given in Table 2.The cane yield had a significant positive association with the number of tillers at 60 days (0.57) and 90 days (0.39), the plant height (0.51), the single cane weight (0.84), the purity (0.35), and the fibre content (0.35); however, the juice weight had a significant negative association.The important quality trait, i.e., pol%, had a significant positive correlation with the cane diameter, juice weight, juice extraction, Brix value, purity, and single cane weight, and a significant negative association with the fibre content, number of millable canes, and nitrogen balance index.The fibre content had a significant association with the maximum number of 14 traits, including 7 traits with a positive association (number of tillers at 60, 90, and 120 days, plant height, number of millable canes, cane yield, and nitrogen balance index) and 7 traits with a negative association (germination, cane diameter, juice weight, juice extraction, Brix value, pol%, and purity).The other important economic trait, i.e., the juice weight, had a significant positive association with the cane diameter, juice extraction, Brix value, pol%, purity, and single cane weight.Similarly, juice extraction had a significant positive association with the cane diameter and juice weight, Brix value, pol%, purity, and single cane weight, and a significant negative association with the fibre content, number of millable canes, nitrogen balance index, and chlorophyll concentration.
Physiological traits had fewer significant associations with agro-morphological and quality traits.Out of the studied four physiological traits, the nitrogen balance index had significant associations with a maximum of eight traits, including three positive (plant height, fibre content, and nitrogen balance index) and five negative (juice extraction, Brix value, pol%, flavonoid index, and anthocyanin index) associations.The chlorophyll concentration had a significant positive association (0.84) with the nitrogen balance index Plants 2024, 13, 1023 8 of 20 and a significant negative association with juice extraction (−0.37) and purity (−0.36).The anthocyanin index had a significant negative association with the nitrogen balance index and the chlorophyll concentration, and a significant positive association with the flavonoid index.The physiological trait leaf area did not have a significant association with any of the studied traits.Generally, there was a strong and significant positive association among the sugarcane juice quality parameters, including the juice weight, juice extraction, Brix value, pol%, and purity.

Principal Component Analysis (PCA)-Biplot Analysis
The inter-relationship among various traits and genotypes was assessed in the form of biplots in each environment, as shown in Figures 6 and 7.The PCA was performed on 20 traits and measured their contribution to the variability.In the year 2018-2019, five PCs were significant and accounted for 85.27% of the total variation.The first PC captured around 36.6% of the total variation, and that was attributed to the single cane weight, pol%, purity, juice extraction, juice weight, germination, and anthocyanin index for the largest positive loadings.The second PC captured 21.04% of the total variation, majorly contributed from the cane yield, tillers numbers, plant height, fibre content, and numbers of millable canes, with positive loadings.The biplot based on PC1 and PC2 placed the high-yielding genotypes in the first two quarters and also revealed the significant positive correlation of the cane yield with the plant height, single cane weight, and tiller numbers.In the year 2019-2020, seven PCs were significant and represented 89.08% of the total variability.The first PC captured around 28.01% of the variability and was attributed to the positive loadings of the cane diameter, juice weight, germination, and single cane weight, whereas the second component captured 19.5% of the total variability, represented by the cane yield, tiller numbers, plant height, and fibre content.Similar to 2018-2019, the first two-quarters of the biplot based on PC1 and PC2 were occupied by the high-yielding genotypes.
bility.The first PC captured around 28.01% of the variability and was a ributed to the positive loadings of the cane diameter, juice weight, germination, and single cane weight, whereas the second component captured 19.5% of the total variability, represented by the cane yield, tiller numbers, plant height, and fibre content.Similar to 2018-2019, the first two-quarters of the biplot based on PC1 and PC2 were occupied by the high-yielding genotypes.The winter sprouting ability, measured as the winter sprouting index (WSI) of Erinathus procerus derived hybrids and check varieties, is presented in Figure 8A and Table 3.The WSI of all the check varieties was lower than the Erinathus procerus derived hybrids used in the study.The WSI of the commercial check variety ranged from 1.4 to 2.8.However, the Erinathus procerus derived hybrids ranged from 3.4 to 10.6.Among the check varieties, Co 0238 was the best check, followed by CoJ 64.However, the best check (Co 0238) was inferior to the Erinathus procerus derived hybrids.The percent superiority of Erinathus procerus derived hybrids over the best commercial check (Co 0238) is given in Figure 8B.GU 12-19 was the best genotype for winter sprouting ability, with a WSI of 10.6 and a high percent superiority (279%), followed by GU 12-22 (WSI: 8.5 and a 204% superiority over the best check), GU 12-21 (WSI: 7.2 and a 157% superiority over the best check), and GU 12-29 (WSI: 6.9 and a 146% superiority over the best check).Out of the 15 hybrids, nine Erinathus procerus derived hybrids had a better winter sprouting ability over the best check, Co 0238, and the experimental mean (WSI: 3.8); however, the remaining six hybrids had better performance over all the checks and were also on par with the performance of the experimental mean value of WSI, as indicated in Figure 8A.
used in the study.The WSI of the commercial check variety ranged from 1.4 to 2.8.However, the Erinathus procerus derived hybrids ranged from 3.4 to 10.6.Among the check varieties, Co 0238 was the best check, followed by CoJ 64.However, the best check (Co 0238) was inferior to the Erinathus procerus derived hybrids.The percent superiority of Erinathus procerus derived hybrids over the best commercial check (Co 0238) is given in Figure 8B.GU 12-19 was the best genotype for winter sprouting ability, with a WSI of 10.6 and a high percent superiority (279%), followed by GU 12-22 (WSI: 8.5 and a 204% superiority over the best check), GU 12-21 (WSI: 7.2 and a 157% superiority over the best check), and GU 12-29 (WSI: 6.9 and a 146% superiority over the best check).Out of the 15 hybrids, nine Erinathus procerus derived hybrids had a be er winter sprouting ability over the best check, Co 0238, and the experimental mean (WSI: 3.8); however, the remaining six hybrids had be er performance over all the checks and were also on par with the performance of the experimental mean value of WSI, as indicated in Figure 8A.Red rot caused by Colletotrichum falcatum is an important stalk disease of sugarcane, causing substantial yield losses in India and other countries.Host-plant resistance plays a vital role in managing the disease.Traditionally, Saccharum spontaneum has been the main source of red rot and other abiotic stress tolerances.The development of new variations in Colletotrichum falcatum is also a major concern and poses challenges in the cultivation of newer varieties.The exploitation of new and diverse genetic resources is essential to enhance disease resistance.Of late, considerable attention has been given to the incorporation of the wild genus Erianthus, which has been identified as a valuable source of many traits, including red rot resistance.In this study, 15 hybrids were subjected to the screening process against subtropical and tropical isolates of Colletotrichum falcatum.The results of the screening revealed varying levels of resistance among the tested clones.A high level of resistance (R) to red rot against virulent isolates of subtropical Cf08 and Cf09 was observed, indicating their ability to withstand infection and exhibit minimal disease symptoms.The clones were classified as moderately resistant (MR), indicating a moderate level of resistance to the pathogen.On the other hand, the clones were categorised as moderately susceptible (MS), indicating a higher susceptibility to red rot compared to the resistant and moderately resistant clones.The last category includes clones that are classified as susceptible (S), indicating their vulnerability to infection and the development of severe disease symptoms (Table 4).The red rot rating of the F 1 hybrid and BC hybrids against the mixed inoculum of Cf671 and Cf94012 (Colletotricum falcatum) is given in Table 4.The intergeneric hybrids have shown resistant reaction and the back-cross hybrids, viz., GU 12-21, GU 12-23, GU 12-29, and GU 12-31, were also resistant, and other BC hybrids were moderately resistant.

Discussion
The genetic resources of the Erianthus genus consist of seven species; among them, two species, i.e., Erinathus arundinaceus and Erinathus procerus, are the most promising.The genus Erianthus gained significant attention from sugarcane breeders worldwide as a source for high tillering, high ratooning ability, high biomass, and tolerance against potential biotic and abiotic stresses [9,12,15].The Erianthus genus is highly adapted to varied environments and is extensively distributed in both tropical and subtropical regions [16].However, the introgression of Erianthus had limitations due to low compatibility, high genetic distance, and difficulty in identifying the true hybrids from the selfed ones.The work on the utilisation of Erianthus arundinaceus and its introgression into sugarcane for higher biomass has been successful [16][17][18][19].Similarly, the utilisation of Erinathus procerus in sugarcane breeding was very limited, and the work on the intergeneric hybridisation between Saccharum and Erinathus procerus was performed at the ICAR-Sugarcane Breeding Institute, Coimbatore [20].The developed intergeneric hybrids were confirmed using 5S rDNA markers, which can differentiate between Saccharum and Erianthus [7,21,22].The confirmed true intergeneric hybrids were again back-crossed to Saccharum spp. to recover more of the Saccharum genome.A total of 15 such clones derived from the programme of intergeneric hybridisation and back-crossing were evaluated for their agronomic performance, winter ratooning ability, and reaction against red rot resistance.

Variability and Mean Performance for Various Economic Traits
The genotypic effect for all the agro-morphological and quality traits was significant, indicating the importance of Erinathus procerus-derived breeding lines in commercial breeding programmes to improve the economically important traits, such as the tiller number, plant height, cane diameter, and number of millable canes, which ultimately decide the cane yield.The year effect is significant only for six traits, including the fibre content and pol%, which indicated the inconsistent nature of these traits.The interaction was significant for tillers at 90 and 120 days and the fibre content, indicating that the selection for these traits should be done cautiously with multi-environment testing.
A positive association was observed between the cane yield and the number of tillers at 60 and 90 days, the plant height, and the single cane weight; previously, similar results were reported in commercial canes [23].The procerus hybrids were tall-growing, but they were thinner than the commercial canes; hence, in our study, the diameter did not influence the cane yield.However, the juice weight had a significant negative association with the cane yield, indicating more fibre content in the procerus-derived clones.It was concluded that the extraction percentage was also negatively associated with the fibre content and quality traits, including the pol% and Brix value.The genetic variability of the clones for the fibre percentage and its related traits was observed in the Erinathus procerus hybrids [24].
PCA is a convenient statistical technique that found application in the reduction of the original variables into a smaller number of underlying variables to disclose the inter-relationships among various traits and genotypes in each environment, as shown in Figures 6 and 7. Through the biplot analysis, four kinds of information can be inferred, i.e., the relationship among the genotypes, the trait variability, the correlation among traits, and the relative value of the accession to the character.The longer the line in the PCA, the higher the variance.The cosine of the angle between the lines denotes the correlation between the variables.The closer the angle is to 90 or 270 • , the smaller the correlation, and an angle of 0 or 180 • suggests a correlation of 1 or −1, respectively [25,26].

Winter Sprouting Potential
The winter sprouting ability, measured as the winter sprouting index (WSI), is very important for sugarcane varieties grown in subtropical regions.Unlike tropical climates, subtropical regions face many climatic vagaries, viz., high temperature and water-deficit stress during summer and chilling injury-cold stress during winter [27].The crop is also affected by water-logging during the rainy season.Sugar mills in the subtropical regions start cane crushing in mid-November; therefore, the harvesting of the crop starts in November and continues until January for the early crushing periods; this period coincides with peak winter.The harvesting of early-maturing sugarcane varieties in the region starts during the peak winter period when temperatures come down near 2-7 • C. The harvesting of the cane during this period reduces their sprouting due to the chilling weather conditions prevailing over a two-month period.The harsh winter reduces the crop ratooning ability due to lack of sprouting in low temperatures (4-10 • C) and is one of the main reasons for the low productivity in the ratoons of the subtropics [28]; moreover, farmers are not able to harvest the full potential of the ratoon crop.Any sugarcane genotypes that sprout during such chilling temperatures, harbour cold tolerant genes, and such genotypes are considered as highly winter tolerant with a good ratooning potential.The introgression of cold-tolerant genes conferring a high ratooning ability from allied genera is a promising alternative to the breeding of suitable cultivars with a better winter ratooning ability.In the present study, Erianthus procerus-derived hybrids were evaluated for their winter sprouting potential in subtropical climates.The nine procerus hybrids demonstrated a notably superior winter sprouting ability compared to the top-performing check Co 0238.The genotypes GU 12-19, GU 12-22, and GU 12-21 reported a superior WSI and will serve as an alternate source of donor parent to introgress the winter sprouting ability in subtropical varieties and enhance the better ratooning ability of the commercial clones under cold-stress conditions.The identified genotypes with a higher winter ratooning potential could serve as a better option for the sugarcane breeder to utilise these clones in breeding programmes to improve winter tolerance in the cultivated sugarcane varieties.The ultimate aim of the breeder is to improve the productivity of the sugarcane varieties under challenging environmental conditions.The earlier study with 632 sugarcane germplasms revealed a significant and positive correlation of the winter sprouting index (WSI) with the numbers of tillers and millable canes in the ratoon crop [14,[29][30][31].The highest WSI of 6.5 was also reported, and in this study, five clones had a WSI of more than 6.5, indicating the superior winter ratooning ability of the Erinathus procerus-introgressed hybrids.This study also further confirmed the utilisation of the WSI to assess the winter ratooning ability of sugarcane clones and identified the noteworthy procerus hybrids for better ratooning potential [27,32,33].Our study further strengthened sugarcane breeding toward the identification of the desired high winter sprouting donors, which will be utilised in the future for the improvement of the high winter ratoonability under challenging environments.

Red Rot Resistance
Red rot in sugarcane is the major problem of sugarcane cultivation in the subtropics and is responsible for the loss of several elite varieties from cultivation [34].In the present study, the Erinathus procerus derived hybrids were also screened for their reaction against tropical and subtropical isolates of the red rot pathogen.The hybrid GU04 (28) EO-2 and the back-crossed hybrids GU12-19, GU12-21, GU12-22, GU12-23, GU12-26, GU12-27, GU12-30, and GU12-31 were R to MR for the tested isolates, indicating their broad-spectrum resistance against red rot.Three clones, viz., GU 12-19, GU 12-21, and GU 12-22, showed both an excellent winter ratooning ability and broad-spectrum red rot resistance, indicating their potential to improve both traits.Further crossing of GU 12-21 with commercial clones revealed that more than 60% of the progenies were resistant to red rot, suggesting the valuable potential of the clones in imparting red rot resistance.Similar findings were also reported in Erinathus arundinaceus introgressed clones with cold tolerance and red rot resistance [31,35].The Erinathus procerus derived hybrids proved to be the wealthiest pre-bred lines, which can be utilised in the sugarcane improvement programme.Their high biomass nature, high tillering ability, winter sprouting ability, high fibre content, and red rot resistance give new hope to the further enhancement of the winter ratooning ability and red rot resistance in commercial sugarcane varieties with a broadened genetic base.

Fibre and Flavonoids in Erinathus procerus-Derived Hybrids
The fibre in sugarcane is important in the modern world as a source of energy to utilise in cogeneration, the production of second-generation bioethanol, biogas, and pyrolysis to produce bio-oil and biochar [36][37][38].In our study, seven Erinathus procerus hybrids recorded more than 22% fibre, indicating that their proportion of fibre content could be processed to produce fibre suitable for weaving into textiles, such as bags, clothing, and home textiles, making them more suitable for industrial applications and bioenergy production [39].Cane with a high fibre content can be utilised in the production of biodegradable materials, such as bioplastics and packaging, offering sustainable alternatives to traditional plastic products.
In the study clones, CoJ 64 and GU12-30 exhibited a higher flavonoid index, exceeding 1.14 in the year 2018-2019, while GU 12-21 showed superior flavonoids (>1.23) in the year 2019-2020.The significance of flavonoids in sugarcane has been previously documented by Colombo et al. [40].Similarly, hybrids GU12-22 and Co 0238 exhibited a higher anthocyanin index, exceeding 0.147 in the year 2018-2019, while GU12-30 and GU12-38 showed a better anthocyanin index (>0.144) in the year 2019-2020.The role of anthocyanin accumulation in cool weather is crucial, acting as antioxidants against cold injury.Anthocyanin accumulation is positively correlated with antioxidant capacity, protecting against cold stress [20,41].Anthocyanin also plays an inhibitory role in the production of free radicals and reducing the levels of reactive oxygen species (ROS) under low-temperature stress [42].

Experimental Site
The experiment was conducted at the ICAR-Sugarcane Breeding Institute, Regional Centre, Karnal (Haryana) India, which is located at 29.1-29.5 • N and 76.3-77.1 • E in a subtropical climate, with an elevation of 243 m above the mean sea level and an average annual rainfall of 744 mm.In summer, the maximum temperature ranges from 34 to 45 • C, while in winter, the minimum temperature ranges from 5 to 8 • C. The soil in this area varies from clay-loamy to loam, with a pH range of 8.0-8.5.

Plant Material and Experimental Design
A total of 15 clones of Erinathus procerus-derived hybrids were evaluated along with five standard sugarcane varieties, i.e., Co 0238, CoJ 64, CoS 8436, CoS 767, and Co 06027, for their cane yield, physiological traits, and winter sprouting potential.Also, the experimental material was evaluated for both tropical and subtropical isolates of red rot resistance.A list of the Erinathus procerus-derived hybrids along with their parentages are provided in Table 5.The date of planting for the plant crop was in the first week of March during the 2018-2019 crop season and harvested during March-April during 2019-2020.Similarly, the ratoon crop was raised in 2019-2020 during the spring season (Feb-March).The experiment was carried out over a span of two years (the 2018-2019 and 2019-2020 spring seasons) and three seasons for the WSI using a randomised block design with three replications.Plots utilised had dimensions of 2 rows × 3 m and were spaced 0.9 m apart.Standard recommended practices were followed for crop production.Three lifesaving irrigations were applied at the time of germination, tillering, and the grand growth stage of the crop; otherwise, the crop was managed with natural rainfall throughout the year.The observations were collected from each plot, specifically for the number of tillers and the numbers of millable canes, the Brix value, the single cane weight, the plant height, and juice quality parameters [15,35,[43][44][45][46] and physiological parameters like the chlorophyll content, nitrogen balance index (NBI), and flavonoid and anthocyanin index, were recorded from the 10-month-old crop.Five randomly selected canes from each plot were tagged and assessed for the estimation of the fibre percentage; 250 g subsamples of shredded canes were crushed in a rapipol machine and subsequently oven-dried.The fresh and dry To compute the fresh biomass yield (measured in tonnes per hectare, t/ha), the formula used was fresh biomass yield = NMC/ha × SCWT (kg) with the green cane top.

Red Rot Evaluation
To assess their resistance to red rot, a comprehensive screening process was conducted on all Erianthus procerus-derived hybrids.This study aimed to evaluate their resistance against the predominant and highly virulent isolates of red rot, Colletotrichum falcatum, specifically Cf08 and Cf09 in the subtropics and cf671 and cf671 + cf94012 in the tropics [35,47].The screening was performed under field conditions.To initiate the screening, a mixed inoculum containing both isolates of Colletotrichum falcatum was prepared.This inoculum was then applied to the sugarcane plants through plug and nodal methods.The screening was conducted when the crops were approximately seven months old, with inoculation occurring in September.

Measurement of Physiological Parameters
The leaf analyser Dualex Scientific+ (Force-A, Orsay, Paris, France) was used to estimate the polyphenol and chlorophyll content of the leaves based on the absorbance and transmittance of specific wavelengths of visible and near-infrared light [44].The instrument does not directly measure these chemical concentrations, but measures the reflectance and absorbance indices that have been demonstrated to relate to the leaf-level concentrations.The nitrogen balance index and flavonoid, chlorophyll, and anthocyanin contents were observed from the top visual dewlap (TVD) of three different plants.Erianthus-specific markers were developed from 5S rRNA spacer regions using a sequence-tagged PCR.An Erianthus procerus clone along with randomly selected intergenic hybrids and the Saccharum spp.hybrid Co 86032 (commercial variety) were screened with primers that amplified the 5S rDNA regions in the genomes to identify the Erianthus-specific fragments (Figure 9).The PCR amplification conditions for the 5S rDNA of D'Hont et al. [7] were followed.Amplified products were resolved on 2% agarose gels stained with ethidium bromide and documented using a gel documentation system (Syngene, Bangalore, India).Nitrogen Balance Index = Chlorophyll Flavonoids

Confirmation of Intergeneric Hybrids
Erianthus-specific markers were developed from 5S rRNA spacer regions using a sequence-tagged PCR.An Erianthus procerus clone along with randomly selected intergenic hybrids and the Saccharum spp.hybrid Co 86032 (commercial variety) were screened with primers that amplified the 5S rDNA regions in the genomes to identify the Erianthus-specific fragments (Figure 9).The PCR amplification conditions for the 5S rDNA of D'Hont et al. [7] were followed.Amplified products were resolved on 2% agarose gels stained with ethidium bromide and documented using a gel documentation system (Syngene,Bangalore,India).

Statistical Analysis
The collected data underwent analysis of variance (ANOVA) tests for statistical evaluation [48].The statistical package SAS 9.3 software (SAS Institute Inc., Cary, NC, USA) was utilised to calculate the means, standard deviations, and coefficients of variance for various traits [49].

Statistical Analysis
The collected data underwent analysis of variance (ANOVA) tests for statistical evaluation [48].The statistical package SAS 9.3 software (SAS Institute Inc., Cary, NC, USA) was utilised to calculate the means, standard deviations, and coefficients of variance for various traits [49].

Conclusions
The longevity of the sugarcane cultivar in the subtropical regions depends on the winter sprouting potential and broad-spectrum red rot resistance, along with the yield and quality.This study identified two potential clones (GU12-19 and GU12-21) for their high WSI and red rot resistance.Further, GU12-19 contains >22.0%fibre, making it more suitable for industrial applications and bioenergy production.Erianthus procerusderived hybrids exhibited superior performance for physiological parameters, including anthocyanin, which plays a vital role in mitigating cold injury.The cultivated sugarcane hybrids had a narrow genetic base for tolerance to cold injury and winter sprouting ability in red rot resistance genetic backgrounds.Hence, the Erianthus procerus-derived hybrids proved to be the wealthiest pre-bred lines, which can serve as a potential source to be utilised in future breeding programmes to develop sugarcane cultivars with red rot resistance and a high winter ratooning potential in subtropical conditions.

Supplementary Materials:
The following supporting information can be downloaded at: https: //www.mdpi.com/article/10.3390/plants13071023/s1,Table S1: Mean performance of E. procerus derived hybrids and standard varieties during the year 2018-2019.Table S2.Mean performance of E. procerus derived hybrids and standard varieties during the year 2019-2020.
Author Contributions: M.R.M. and K.M. conceived and designed the experiments.M.R.M., R.K., N.K., K.M. and M.L.C. laid out the trials and collected the data.R.A.K., H.K.M.S., G.K., G.H., P.G., A.S. and R.A.K. processed and analysed the data.All authors discussed, edited, and approved the final manuscript.All authors have read and agreed to the published version of the manuscript.The threshold set in a measurement which indicates the maximum acceptable value for a particular parameter

LDL: Lower decision limit
The threshold set in a measurement which indicates the minimum acceptable value for a particular parameter BC: Back-cross Mating of a hybrid organism (offspring of genetically unlike parents) with one of its parents

Figure 1 .
Figure 1.The weather parameters during January 2018 to December 2020.

Figure 1 .
Figure 1.The weather parameters during January 2018 to December 2020.

Funding:
This work was supported by the ICAR-Sugarcane Breeding Institute, Coimbatore, India.Ger: Germination (%) Percentage of cane bud setts in a given sample that have successfully begun to emerge out NBI: Nitrogen balance index The ratio between chlorophyll and flavonoid contents and indicator of crop N status for crop growth 60-day tillers ('000/ha) Population of tillers after 60 days of a specific crop's growth 90-day tillers ('000/ha) Population of tillers after 90 days of a specific crop's growth 120-day tillers ('000/ha) Population of tillers after 120 days of a specific crop's growth PH: Plant height (cm) Vertical growth of cane from the base to the top of the stem, where it easily breaks by hand CD: Cane diameter (cm) Measurement of the diameter of a cane stalk NMCs: Number of millable canes ('000/ha) The population of mature cane which is ready to crush in the sugar factory Leaf area (cm 2 ) The surface area of a leaf and expressed in square centimetres.Leaf area (cm 2 ) = length (cm) × width (cm) CHLORO: Chlorophyll (µg/cm 2 ) Amount of chlorophyll present in a unit area of a cane leaf surface CY: Cane yield (t/ha) Amount of sugarcane (in weight) harvested from a specific area WSI: Winter sprouting index WSI = percentage of sprouted clumps × number of shoots per clumps/100 SPAD: Soil plant analysis development Measurement of the relative chlorophyll content in leaves in a non-destructive way TVD: Top visual dewlap Uppermost fully expanded leaf with visible dewlap or distinct collar, a band between the leaf sheath and leaf blade PCR: Polymerase chain reaction Used to amplify a specific segment of DNA in millions to billions of copies UDL: Upper decision limit

Table 1 .
Pooled analysis of variance for agro-morphological, quality, and physiological traits in Erianthus procerus clones and check varieties.

Table 2 .
Correlation matrix among cane growth parameters and physiological and quality traits.

Table 4 .
Reaction of Erinathus procerus derived clones for red rot against tropical and subtropical isolates during 2018-2019 and 2019-2020.