Interactive Effect of Arbuscular Mycorrhizal Fungi (AMF) and Olive Solid Waste on Wheat under Arsenite Toxicity

Heavy metal such as arsenite (AsIII) is a threat worldwide. Thus, to mitigate AsIII toxicity on plants, we investigated the interactive effect of olive solid waste (OSW) and arbuscular mycorrhizal fungi (AMF) on wheat plants under AsIII stress. To this end, wheat seeds were grown in soils treated with OSW (4% w/w), AMF-inoculation, and/or AsIII treated soil (100 mg/kg soil). AMF colonization is reduced by AsIII but to a lesser extent under AsIII + OSW. AMF and OSW interactive effects also improved soil fertility and increased wheat plants’ growth, particularly under AsIII stress. The interactions between OSW and AMF treatments reduced AsIII-induced H2O2 accumulation. Less H2O2 production consequently reduced AsIII-related oxidative damages i.e., lipid peroxidation (malondialdehyde, MDA) (58%), compared to As stress. This can be explained by the increase in wheat’s antioxidant defense system. OSW and AMF increased total antioxidant content, phenol, flavonoids, and α-tocopherol by approximately 34%, 63%, 118%, 232%, and 93%, respectively, compared to As stress. The combined effect also significantly induced anthocyanins accumulation. The combination of OSW+AMF improved antioxidants enzymes activity, where superoxide dismutase (SOD, catalase (CAT), peroxidase (POX), glutathione reductase (GR), and glutathione peroxidase (GPX) were increased by 98%, 121%, 105%, 129%, and 110.29%, respectively, compared to AsIII stress. This can be explained by induced anthocyanin percussors phenylalanine, cinamic acid and naringenin, and biosynthesic enzymes (phenylalanine aminolayse (PAL) and chalcone synthase (CHS)). Overall, this study suggested the effectiveness of OSW and AMF as a promising approach to mitigate AsIII toxicity on wheat growth, physiology, and biochemistry.


Introduction
Plant growth and productivity are adversely affected by metal and metalloid stress worldwide. Anthropogenic activity resulted in heavily-contaminated soils with metals and stress [23]. A variety of microorganisms that promote plant growth, including endophytic fungi, AMF, and plant-growth-promoting rhizobacteria, have been demonstrated to increase plant growth during stress by assessing soil fertility and structure and providing nutrients to the host plant [24]. Furthermore, AMF has been shown to increase development under harsh situations such as abiotic stress [13]. AMF is an important bio-ameliorator of stress, increasing stress tolerance and changing root architecture to enhance access to nutrients and water [25]. AMF may also boost photosynthetic activity in host plants by influencing physiological factors [25].
The second most important cereal crop worldwide is wheat (Triticum aestivum L.). Wheat plants may grow in a variety of climate zones (from tropical to temperate). However, several abiotic stressors, such as heavy metal toxicity impact wheat plant production and productivity. To this end, this study aimed to study As III toxicity on plant growth, physiology and defense systems. Moreover, interaction with AMF application is essential for maximizing AMF's participation in phytoremediation techniques. However, limited research has identified various harmful effects of As III on plant shoot development. Arsenite a result, the present research looks at the impact of AMF inoculation on wheat growth in As III -contaminated soil. In the present study, treatment with OSW and AMF (Rhizophagus irregularis) would benefit the host plant by reducing As III toxicity on growth and metabolism. However, the physiological and biochemical bases of the As III stress mitigation impact of OSW and AMF are not well studied. Therefore, this study aimed to understand how the interaction between AMF and OSW can improve wheat plant growth and reduce As III -induced oxidative damages. To this end, photosynthesis related parameters, ascorbate-glutathione cycle, antioxidant metabolites, and enzymes and anthocyanin metabolism were investigated. Overall, this will acquire a better understanding of the underlying processes that control plant response to As III toxicity.

AMF Colonization
Heavy metal stress impacts microbial ecology, particularly in the rhizosphere. Arsenite a result, the rhizosphere microbiome is constantly changing to adapt to environmental changes. Mycorrhiza colonization in the roots and rhizosphere of wheat plants was tracked to assess AMF colonization under As III stress and normal conditions (Table 1). Infection was seen in AMF-treated wheat plants, as predicted. However, compared to AMF treatment, root colonization, hyphal length, and arbuscules numbers decreased by 45,49, and 40%, respectively, under As + AMF treatment but to a lesser extent under As + OSW+AMF treatment i.e., 40,43, and 51%, respectively (Table 1). These results suggested that the AMF could colonize plants roots when subjected to As III stress.

Growth Analysis
We first tested if AMF and OSW could benefit wheat growth. We therefore quantified fresh weight (FW) and dry weight (DW) biomass production of wheat shoot. Wheat plant's growth parameters, like fresh shoot weight and dry weight, when As III , OSW, AMF, and their combination (OSW + AMF) were applied ( Figure 1). Applying As III stress to wheat plants decreases growth parameters, such as fresh shoot weight and dry weight, which were reduced by approximately 37% and 59%, respectively, compared to non-stressed plants. OSW, AMF, and OSW + AMF, on the other hand, considerably enhanced growth parameters in As III stressed plants compared to non-stressed plants. Plants treated with OSW+AMF showed the most significant increases. Consequently, the fresh weight of the shoot of plants grown in OSW+AMF-treated soil was increased by about 112% and 117% compared to the As III stress. We first tested if AMF and OSW could benefit wheat growth. We therefore quantified fresh weight (FW) and dry weight (DW) biomass production of wheat shoot. Wheat plant's growth parameters, like fresh shoot weight and dry weight, when As III , OSW, AMF, and their combination (OSW+AMF) were applied ( Figure 1). Applying As III stress to wheat plants decreases growth parameters, such as fresh shoot weight and dry weight, which were reduced by approximately 37% and 59%, respectively, compared to nonstressed plants. OSW, AMF, and OSW+AMF, on the other hand, considerably enhanced growth parameters in As III stressed plants compared to non-stressed plants. Plants treated with OSW+AMF showed the most significant increases. Consequently, the fresh weight of the shoot of plants grown in OSW+AMF-treated soil was increased by about 112% and 117% compared to the As III stress. Figure 1. The influence of olive solid waste (OSW) and arbuscular mycorrhizal fungi (AMF), combination (OSW+AMF) on (a) fresh weight of shoot and (b) dry weight of shoots in wheat plants growing under As III stress. Fishers test at p < 0.05 reveals significant variations in means (± standard deviation), which are different letters on the same bars. Figure 1. The influence of olive solid waste (OSW) and arbuscular mycorrhizal fungi (AMF), combination (OSW + AMF) on (a) fresh weight of shoot and (b) dry weight of shoots in wheat plants growing under As III stress. Fishers test at p < 0.05 reveals significant variations in means (±standard deviation), which are different letters on the same bars.

Photosynthetic Rate and Pigments Contents
To investigate how AMF and OSW are able to stimulate biomass production in the presence of substantial As III bioaccumulation, we quantified key parameters related to photosynthesis (total chlorophyll, carotenoid, and photosynthetic rate). Photosynthesis rate process, chlorophyll a, b, a + b, and carotenoids content in leaves of wheat plants show significant decreases of about 56%, 42.11%, 55%, 44%, and 27%, respectively, compared to control plants ( Figure 2). In addition, the photosynthesis process, Chl a, Chl b, Chl a+b, and carotenoid content showed the highest values of about 169%, 147%, 247%, 159%, and 106%, respectively, after treatment with a combination of OSW+AMF compared to As III stress plants followed by AMF and OSW. This significant decrease in photosynthetic activity upon As III exposure could lie at the basis of reduced shoot biomass.
106%, respectively, after treatment with a combination of OSW+AMF compared to As III stress plants followed by AMF and OSW. This significant decrease in photosynthetic activity upon As III exposure could lie at the basis of reduced shoot biomass.

Oxidative Damage in Wheat Plants under As III Stress
As III toxicity is an important aspect of oxidative stress. Consequently, we investigated individual parameters related to oxidative damage. A significant observation on ROS generation (H2O2) and the formation of MDA included an indication of lipid peroxidation in wheat leaves treated with OSW, AMF, and a combination (OSW + AMF), in the presence or absence of As III stress ( Figure 3). Our findings reveal that exposing wheat plants to As III stress, induced by increased As III , levels resulted in a considerable rise in H2O2 (179%) content compared to control plants. This led to increased lipid peroxidation (MDA 80%). On the other hand, using OSW, AMF, and a combination OSW+AMF, showed a significant capacity to ameliorate As III stress by lowering the H2O2 and MDA levels. In comparison, a combination of OSW+AMF under As III stress resulted in a very significant drop in H2O2 (41%) and MDA (58%) content.

Oxidative Damage in Wheat Plants under As III Stress
As III toxicity is an important aspect of oxidative stress. Consequently, we investigated individual parameters related to oxidative damage. A significant observation on ROS generation (H 2 O 2 ) and the formation of MDA included an indication of lipid peroxidation in wheat leaves treated with OSW, AMF, and a combination (OSW + AMF), in the presence or absence of As III stress ( Figure 3). Our findings reveal that exposing wheat plants to As III stress, induced by increased As III , levels resulted in a considerable rise in H 2 O 2 (179%) content compared to control plants. This led to increased lipid peroxidation (MDA 80%). On the other hand, using OSW, AMF, and a combination OSW+AMF, showed a significant capacity to ameliorate As III stress by lowering the H 2 O 2 and MDA levels. In comparison, a combination of OSW + AMF under As III stress resulted in a very significant drop in H 2 O 2 (41%) and MDA (58%) content.  . Fishers test at p < 0.05 reveals significant variations in means (± standard deviation), which are different letters on the same bars.

Antioxidant Defense System
Antioxidant Metabolites Next, we aimed to gain a more in-depth understanding of the biochemical processes that lie at the basis of the response to AMF and OSW under As III exposure. In As III -stressed plants, the antioxidant metabolites (i.e., total antioxidant content, phenol, flavonoids, and α-tocopherol) increased significantly compared to the non-As plant. Treatment wheat plants with 100 mg/kg As III resulted in significant increases in total antioxidant, phenol, flavonoids, and α-tocopherol content of approximately 87%, 64%, 62%, 141%, and 54%, respectively, compared to untreated plants ( Figure 4). The most pronounced increases in relieving stress were observed when combination (OSW+AMF) treatment was used, followed by treatment with AMF and OSW. In As III stressed wheat plants, antioxidant metabolites (i.e., total antioxidant content, phenol, flavonoids, and α-tocopherol) were significantly increased by approximately 33%, 63%, 118%, 232%, and 92%, respectively, compared to As III stressed plants ( Figure 4). The activities of the ASC/GSH pathway at metabolities (ASC, GSH, GSH/TGSH) and enzymes activities (DHAR, MDHAR, GSH) were markedly increased in wheat shoots under As III stress compared to As III -stressed plants without AMF and OSW treatment ( Figure 5). In addition, there was a highly significant increase in ASC levels and DHAR, MDHAR, and GSH enzymes' activities in plants treated with combination (OSW+AMF), as compared with As stress plants ( Figure  5).

Antioxidant Defense System Antioxidant Metabolites
Next, we aimed to gain a more in-depth understanding of the biochemical processes that lie at the basis of the response to AMF and OSW under As III exposure. In As III -stressed plants, the antioxidant metabolites (i.e., total antioxidant content, phenol, flavonoids, and α-tocopherol) increased significantly compared to the non-As plant. Treatment wheat plants with 100 mg/kg As III resulted in significant increases in total antioxidant, phenol, flavonoids, and α-tocopherol content of approximately 87%, 64%, 62%, 141%, and 54%, respectively, compared to untreated plants ( Figure 4). The most pronounced increases in relieving stress were observed when combination (OSW+AMF) treatment was used, followed by treatment with AMF and OSW. In As III stressed wheat plants, antioxidant metabolites (i.e., total antioxidant content, phenol, flavonoids, and α-tocopherol) were significantly increased by approximately 33%, 63%, 118%, 232%, and 92%, respectively, compared to As III stressed plants ( Figure 4). The activities of the ASC/GSH pathway at metabolities (ASC, GSH, GSH/TGSH) and enzymes activities (DHAR, MDHAR, GSH) were markedly increased in wheat shoots under As III stress compared to As III -stressed plants without AMF and OSW treatment ( Figure 5). In addition, there was a highly significant increase in ASC levels and DHAR, MDHAR, and GSH enzymes' activities in plants treated with combination (OSW+AMF), as compared with As stress plants ( Figure 5).

Effects of OSW, AMF, and Combination (OSW+AMF) Application on Antioxidant Stressed Plants Were Enzymes in Wheat Plants under As III Stress
In addition to the ASC/GSH cycle that lies at the core of the plant's ability to metabolize ROS, direct ROS-detoxifying enzymes (SOD, CAT, POX, GR, and GPX) are well known in reducing ROS accumulation in stressed plants. When As III compared to nonstressed plants, SOD, CAT, POX, GR, and GPX activity significantly increased in the wheat plant leaves ( Figure 6). However, the activities of SOD, CAT, POX, ASC, GR, and GPX enzymes were increased significantly due to the treatments (i.e., OSW, AMF, and combination (OSW+AMF)) compared to As III -stressed plants. The combination

Effects of OSW, AMF, and Combination (OSW+AMF) Application on Antioxidant Stressed Plants Were Enzymes in Wheat Plants under As III Stress
In addition to the ASC/GSH cycle that lies at the core of the plant's ability to metabolize ROS, direct ROS-detoxifying enzymes (SOD, CAT, POX, GR, and GPX) are well known in reducing ROS accumulation in stressed plants. When As III compared to nonstressed plants, SOD, CAT, POX, GR, and GPX activity significantly increased in the wheat plant leaves ( Figure 6). However, the activities of SOD, CAT, POX, ASC, GR, and GPX enzymes were increased significantly due to the treatments (i.e., OSW, AMF, and combination (OSW+AMF)) compared to As III -stressed plants. The combination (OSW+AMF) mitigated the adverse effects of As III stress, where the SOD activity was increased by 97%, CAT raised by 121%, POX activity was increased by 105%, GR activity was raised by 129%, and GPX activity was raised by 110% compared to As III stress ( Figure 6). (OSW+AMF) mitigated the adverse effects of As III stress, where the SOD activity was increased by 97%, CAT raised by 121%, POX activity was increased by 105%, GR activity was raised by 129%, and GPX activity was raised by 110% compared to As III stress ( Figure  6).

Anthocyanin Metabolism in Wheat Plants under As III Stress
In As III -stressed plants, anthocyanin level and metabolism (PAL enzyme activity and cinnamic acid, chalcone, and naringenin levels) were increased significantly compared to the non-stressed plants (Figure 7). Stressed wheat plants with 100 mg/kg As III show sig-

Anthocyanin Metabolism in Wheat Plants under As III Stress
In As III -stressed plants, anthocyanin level and metabolism (PAL enzyme activity and cinnamic acid, chalcone, and naringenin levels) were increased significantly compared to the non-stressed plants (Figure 7). Stressed wheat plants with 100 mg/kg As III show significant increases in anthocyanin level. These increases prompted us to evaluate the changes in biosynthesis intermediates of anthocyanins (phenylalanine, cinnamic acid, and naringenin content) as well as key biosynthetic enzymes (PAL and CHS) (Figure 7). In this regard, anthocyanin, phenylalanine, PAL, cinnamic acid, and naringenin content were increased by approximately 44%, 32%, 43%, 44%, 40%, and 63% respectively, compared to untreated plants (Figure 7). The combination (OSW+AMF) mitigated the adverse effects of As III stress, where the Anthocyanin levels were increased by 48%, phenylalanine by 86%, PAL activity was raised by 95%, cinnamic acid content increased by 101%, chalcone raised by 50%, and naringenin raised by 95% compared to As III stress (Figure 7). regard, anthocyanin, phenylalanine, PAL, cinnamic acid, and naringenin content were increased by approximately 44%, 32%, 43%, 44%, 40%, and 63% respectively, compared to untreated plants (Figure 7). The combination (OSW+AMF) mitigated the adverse effects of As III stress, where the Anthocyanin levels were increased by 48%, phenylalanine by 86%, PAL activity was raised by 95%, cinnamic acid content increased by 101%, chalcone raised by 50%, and naringenin raised by 95% compared to As III stress (Figure 7).

Discussion
In the present study, As III contamination at an applied level (100mg/Kg soil) negatively affected wheat growth and yield by limiting nutrient uptake and overall plant de-

Discussion
In the present study, As III contamination at an applied level (100 mg/Kg soil) negatively affected wheat growth and yield by limiting nutrient uptake and overall plant development. This agrees with previous findings [6]. Arsenic is not an essential nutrient but could be taken up by the plant roots and translocated to different parts of the plant [25,26]. As noted by Wu et al., [6], As is a chemical analogue of P; hence, it is taken up by plant roots via P transporters. By its accumulation in plant tissues, this hazardous element has the potential to enter the food chain and cause harmful effects to human and animal health [27]. Its availability in the soil inhibits plant growth development by limiting root growth and nutrient uptake [28]. This is achieved through As-induced inhibition of root extension, proliferation, and interference with critical metabolic and cellular processes [29]. Therefore, it is important to reduce As toxicity in wheat plants [30].
In the current study, wheat plants were cultivated in As III -contaminated soil without or with OSW and/or AMF at plant growth. These results are consistent with earlier studies that found As III toxicity in a variety of plant species [31]. The deleterious effects of As III on plant growth, mineral accumulation, and physiology might explain this decrease in wheat development [32]. Our results demonstrated that AMF inoculation positively influenced plant growth traits. A combination of OSW + AMF has various effects on plant growth under harsh conditions [33]. Changing the metal species in the soil, a combination of OSW+AMF may minimize metal build-up in plants, resulting in increased plant growth and biomass. Under experimental conditions, adding a combination of OSW + AMF boosted the development of As III -stressed wheat. In this research, OSW and/or AMF enhanced shoots' fresh and dry weights in wheat plants under both control and under As III stress. However, at 100 mg/kg, the As III form proved poisonous to the wheat plants, causing a significant growth decline. Vejvodová et al., [15] showed biomass losses in wheat under As stress, as well as those that showed OSW and/or AMF alleviating As III -toxic effects on plant growth. Furthermore, As III negatively influenced AMF colonization by lowering root colonization levels, suggesting fungal toxicity.
To further understand the impacts of As III stress on the plant-OSW and/or AMF systems, we used an approach that considers how soil metal concentrations might influence plant metal concentrations either directly via root absorption or indirectly through effects on AMF colonization (Table 1). AMF colonization was evidently considerably decreased in both examined plants in response to As III toxicity. AMF may resist metal stress by reducing the intake of non-essential metals or by sequestering metals in cell walls or intracellular compartments to elicit cell damage [34]. AMF may also influence metal mobility and availability in soil by generating exudates that chelate or bind metals [35]. AMF, for example, may influence plant metal intake by either decreasing plant uptake or sequestering metals in their own tissues or enhancing plant uptake by actively translocating metals [34]. Depending on the colonization state of the plants, AMF may either ease or worsen the toxicity of As III stress in plants. The link between AMF colonization and plant metal absorption, on the other hand, is not always linear [36]. Consequently, synthesizing these divergent findings across multiple plant-metal systems is challenging, and most do not explore the potential that soil metals influence plants directly and indirectly via AMF [34].
Arsenite triggers the production of ROS, which have detrimental impacts on plants at biochemical, physiological, and molecular levels. The role of different enzymatic (SOD, CAT, GR, and APX) and non-enzymatic (salicylic acid (SA), proline, phytochelatins, GSH, nitric oxide, and phosphorous) substances under As stress has been defined via conceptual models showing As III translocation and toxicity pathways in plants [12]. Several biochemical compounds such as chlorophyll, CAT, and proline were found to be significantly changed in mung bean crops under As III stress [37]. The changes in these compounds negatively impact the growth and development of mung bean crops and other food crops. Among these parameters, increases in the chlorophyll enhance photosynthesis and increase the green color in food crops. OSW, AMF, and combination OSW+ AMF have all shown that our As III research significantly improves all photosynthetic criteria. Zhang et al. [38] results are corroborated by OSW and/or AMF, which reveal that an increase in biosynthetic pathway intermediates and metabolites, necessary for chlorophyll synthesis under As stress is the sole component leading to an increase in photosynthetic rate and efficiency. Furthermore, enhanced photosynthetic rate, sugar build-up, and reduced oxidative stress have all been employed to illustrate OSW's efficacy in heavy metal stress conditions [39]. The amount of As III pollution in the soil and pigment concentrations were considerably higher in OSW-and/or AMF-treated plant shoots. Chl a and Chl b concentrations in OSW and/or AMF plants have been observed to be higher in citrus, cucumber, and chickpea plants [39].
When exposed to As III stress, a greater concentration of total carotenoids (OSW + AMF) than AMF and OSW imply that Chl is protected from As III -induced oxidative damage. Extensive reactive oxygen species (ROS) occur during As III stress, just as they do under other stressful conditions. Arsenite a result, large levels of oxidative biomarkers such as MDA and H 2 O 2 were dramatically elevated in response to environmental challenges and employed as a positive criterion to assess plant resistance to As III stress. This research found that all oxidative indicators were reasonably low by using OSW, AMF, and a mixture of the levels seen in As III -stressed plants. Physiologically, the higher frequency of lipid peroxidation and H 2 O 2 concentration in wheat plants has been linked to enhanced plasma membrane breakdown and cytoplasm dryness caused by severe As III stress. Furthermore, H 2 O 2 is responsible for the enhanced solute breakdown associated with oxidative stress [40].
OSW and/or AMF have the ability to reduce oxidative stress by preserving plasma membrane integrity, regulating water intake, and enhancing water consumption efficiency [41]. Similar to the positive effect of OSW, is the possible influence of biochar on crop yield and biomass production in bean plants under As III stress conditions on MDA and H 2 O 2 levels [42]. According to Sharma et al. [35], AMF was also indicated to reduce MDA to a minimum level in order to counteract As III stress. Redox equilibrium and the antioxidant defense system are critical in controlling the formation of excessive ROS and the duration of oxidative stress in plants under irregular environmental circumstances [43]. OSW and/or AMF have been identified as a pleiotropic component that directly or indirectly assists in detoxifying excess ROS by reinforcing the antioxidant defense system, resulting in enhanced stress tolerance [43]. In wheat plants treated with OSW and/or AMF in the presence of As III stress, the ascorbate-glutathione cycle enzymes (APX, GR, GST, DHAR, and MDHAR) were also upregulated, which may serve to scavenge high H 2 O 2 by balancing ASC and GSH levels. In addition, the ASC-GSH cycle enzymes' effective actions help to maintain cellular redox equilibrium and prevent oxidative damage [43]. Under high stress, the ASC-GSH pool in cucumber was similarly disrupted [43].
In addition to antioxidant enzymes, polyphenols mitigated As III toxicity by trapping the hydroxyl radical . The flavonoid also acts as an antioxidant and antiradical by chelating transition metals (Fe 2+ ), preventing the Fenton reaction from creating ROS. Consistently, co-cultivation with OSW and/or AMF, as well as As III treatment, boosted the concentration of phenols and flavonoids even more. This increase may be attributed to the activation of the phenylpropanoid pathway through increased PAL activity. We also assessed PAL, the rate-limiting biosynthetic enzyme for phenols and flavonoids, which was enhanced in As III -stressed plants in the presence or absence of OSW and/or AMF treatment, supporting the hypothesis that greater PAL content may aid in boosting phenols and flavonoids contents [44]. In line with our results, CHS transcript levels increased in As IIIstressed plants, and the expression increased even more following melatonin treatment, indicating that CHS may assist in improving anthocyanin concentration and minimizing toxicity [43]. Our findings are also supported by studies of similar melatonin properties in pepper plants exposed to boron poisoning and rosemary herb exposed to arsenic [45]. Furthermore, anthocyanins may behave as the Swiss Army knife of the plant world, defending plants against stress in a number of ways [46]. Similarly, OSW and/or AMF significantly enhanced anthocyanin levels in plants sprouts, demonstrating higher antioxidant activities, reduced ROS formation, and osmotic adjustment indicators (OSW and/or AMF significantly enhanced anthocyanin levels in plants sprouts, demonstrating higher antioxidant activities, reduced ROS formation, and osmotic adjustment indicators [47]. In reaction to stress, anthocyanins quench H 2 O 2 to preserve cell homeostasis. However, the activity of the chloroplastic antioxidant enzymes is decreased at this moment [48]. Notably, anthocyanins have been shown to behave as superoxide anions and H 2 O 2 scavengers in the vacuoles of mesophyll cells [49]. The enzyme chalcone synthase is actively involved in producing anthocyanins [49].

Experimental Set up
In the experiment, 30% soil (300 g) and sterilized 70% sand (700 g), each with a water content of 60%, were mixed with Tref EGO substrates (50 g) in Moerdijk, NL. In pot experiment, soils were supplemented with 4% w/w of solid olive waste and placed into 2 L pots. Olive solid waste was obtained from the three-phase olive oil factory in Al Juef, Saudi Arabia. This dose was selected according to a preliminary experiment testing different concentrations of solid olive waste (0-10%, w/w) on wheat growth. Treated and untreated soil containing solid olive waste was separated into two groups: inoculated with Rhizophagus irregularis (AMF; MUCL 41833). Surface sterilization of the seeds was accomplished using sodium hypochlorite (5% v/v, 20 min). Wheat seeds (Triticum aestivum L., cv Giza 119) were received from the Giza Agriculture Research Center in Egypt. At a humidity of 0.33 g water/g dry soil, the soil originally contained 14.8 mg nitrate-nitrogen (N), 10.7 mg carbon (C), 1.1 mg ammonium-N, and 9.4 mg phosphorus (P)/g air-dry soil. The pH was 7.56, the 3.4 dS/m EC, and the K concentration (2.75 meq/L). Seeds were germinated in T1) control soil, T2) soil treated with olive solid waste (OSW, 4% w/w), T3) AMF-inoculated soil, T4) AMF and OSW treated soil, T5) As III treated soil (as NaAsO 2 at 100 mg/kg soil), T6) soil treated with AS III and OSW, T7) soil treated with AS III + AMF, and T8) soil treated with As III , OSW and AMF (Table 2). Table 2. A list of applied treatments.

T1
Control soil T2 Soil treated with olive solid waste (OSW, 4% w/w) T3 AMF-inoculated soil T4 AMF and OSW treated soil T5 As III -treated soil (as NaAsO 2 at 100 mg/kg soil) T6 Soil treated with As III and OSW T7 Soil treated with As III + AMF T8 Soil treated with As III , OSW and AMF Throughout the experiment, the soil water content was held at 60% (maximum water holding capacity). Based on early studies, a concentration of 100 mg of As III per kg of soil was chosen to excrete stress, which caused a 50% decrease in plant development. Because As III is more poisonous, mobile, and soluble than other As forms, like As V . Plants were cultivated in a climate-controlled room at 21/18 • C with a day/night photoperiod of 16/8 h (60% humidity, 150 mol PAR m2 s1). All pots were set up in a randomized full-block design with five replicates for each treatment. Tap water was used to water the pots daily. The rhizosphere soils and plant shoots and roots were collected after 6 weeks of seed sowing. Plant shoot tissues were collected in liquid N and kept at −80 • C for subsequent biochemical analysis. Soil samples were collected in an icebox and kept at −20 • C for subsequent physical and biological examination. The fresh and dried weights of the harvested shoots and roots were calculated, and AMF related parameters (root colonization, hyphal length, and number of arbuscules) were analyzed.
Fresh roots were rinsed with tap water, split into segments (0.5-1 cm), and cleaned with a 10% KOH solution for each treatment. The roots were then stained with 0.05% trypan blue (SIGMA) in lacto-glycerol and inspected under a stereomicroscope. The colonization was then computed using the gridline intersect technique, and the abundance of arbuscular in the root system was determined by the number of arbuscules cm −1 root [50]. Finally, the hyphal length was calculated using the methods of Tennant, [51] and Rillig et al., [52].

Soil Analysis
By shaking the excavated roots gently, the rhizosphere was separated from the bulk soil, and then soil attached to the fine roots (0-2-mm thick layer) was brushed [38]. described that phenolic content was spectrophotometrically determined for 10 g of the rhizosphere in distilled water using a Shimadzu UV-Vis 1601 PC. In addition, according to [53], mineral nutrients and organic matter (OM) were measured (Table 3). Finally, according to Brown et al. [54], CaCO 3 percentages were estimated. This study used CN element analyzers (NC-2100, Carlo Erba Instruments, Milan, Italy) to determine the C and N contents. Using ribitol as an internal standard, citric acid was extracted in a 0.1% phosphoric acid solution containing butylated hydroxyanisole. De Sousa et al. [55] describe HPLC quantification of the centrifuged filtrates (LaChrom L-7455 diode array, LaChrom, Tokyo, Japan). Nitric acid (HNO 3 ) and hydrochloric acid (HCLO 4 ) were used to digest soil and plant samples to extract total As. After stranding the samples overnight, they were transported to the digestion block at 120 • C and halted when a thick white fume of HCLO 4 was released. By using external calibration, flow injection hydride generation atomic absorption spectrophotometry (FI-HG-AAS, Perkin Elmer A Analyst 400, CITY, USA) was used to analyze the As concentration in plant tissues and soil after digestion [56]. Maximum sensitivity was obtained using 10% HCl and 0.4% NaBH 4 . Table 3. Physico-chemical characterization of olive solid waste (OSW). Three sampels of OSW were analyzed. Values are presented as mean ± SE.

Photosynthesis Related Parameters
Based on data from AbdElgawad et al. [57], we determined the light-saturated photosynthetic rate and gas exchange of developed leaves utilizing the COR LI-6400, LI-COR Inc., Lincoln, NE, USA. To measure pigment concentration, 80% acetone was used to extract pigments [57].

Stress Markers
50 mg tissues (leaves and properly rinsed root discs) were extracted in 1 mL 80% ethanol using MagNALyser to determine lipid peroxidation levels (Roche, Vilvoorde, Belgium). The extract was then tested for malondialdehyde (MDA) using the thiobarbituric acid assay [58]. Protein carbonyls were evaluated as oxidative damage indicators using Cayman Chemical's (Ann Arbor, MI). Protein Carbonyl Colorimetric Assay Kit [59]. The Xylenol orange technique, which relies on peroxide-catalyzed oxidation of Fe 2+, was used to measure hydrogen peroxide (H 2 O 2 ) in trichloroacetic acid (TCA) (0.1%) extracts of plant materials [60]. To rule out non-specific interactions, each sample was matched to its catalase-treated counterpart.

Antioxidant Metabolites
Total antioxidant capacity (ferric reducing antioxidant power, FRAP) was extracted in ice-cold 80% ethanol using a MagNALyser (Roche, Vilvoorde, Belgium) and measured using Trolox as a reference [55]. Plant materials were extracted in 1mL 80% ethanol using MagNALyser for ascorbate and glutathione measurement (Roche, Vilvoorde, Belgium). HPLC was used to measure reduced ascorbate (ASC) and reduced glutathione (GSH). Total ascorbate (ASC + DHA) and glutathione (GSH + GSSG) concentrations were evaluated after dithiothreitol (DTT) reduction, as previously reported [13]. Polyphenols and flavonoids were extracted by homogenizing fresh plant materials in 80% ethanol and centrifuged for 15 min at 5000 rpm. The clear extract was then used to determine the total phenolic and flavonoid content using the Folin-Ciocalteu and aluminum chloride tests.

Statistical Analysis
A completely randomized block design was used in the experiments. The experiments were conducted using a completely randomized block design. SPSS (Chicago, IL, USA) and R were used to analyze the data (Team, 2013). The Kolmogorov-Smirnov (SPSS) and Levene's tests were used to determine data normality and homoscedasticity. Duncan's (SPSS, following two-way ANOVA) was used for the subsequent pairwise statistical comparison of means.

Conclusions
This study aimed to understand how OSW can stimulate the AMF role in As III stress mitigation in wheat plants. Increased AMF colonization under OSW treatment improved growth and physiology of wheat plants, particularly under As III stress. AMF and/or OSW also provides C skeletons for production antioxidants, which might be one of the main reasons for improving plant tolerance to As III toxicity. To understand further the signaling and molecular mechanisms of OSW-induced AMF positive impact under As III stress, underlying detailed metabolomics and transcriptomic studies are needed.