Light Intensity Affects the Assimilation Rate and Carbohydrates Partitioning in Spinach Grown in a Controlled Environment

The cultivation of spinach (Spinacia oleracea L.) has been increasing during the last years in controlled environment agriculture, where light represents a key factor for controlling plant growth and development and the highest energetic costs. The aim of the experiment was to evaluate the plant’s response to two light intensities, corresponding to an optimal and a reduced level, in terms of the photosynthetic process, photoassimilates partitioning, and the biosynthesis of sucrose and starch. Plants of spinach cv. ‘Gigante d’Inverno’ were grown in a phytotron under controlled conditions, comparing two values of photosynthetic photon flux density (PPFD), 800 μmol m−2 s−1 (800 PPFD) and 200 μmol m−2 s−1 (200 PPFD), at a 10 h light/14 h dark regime. Compared to 800 PPFD, under 200 PPFD, plants showed a reduction in biomass accumulation and a redirection of photoassimilates to leaves, determining a leaf expansion to optimize the light interception, without changes in the photosynthetic process. A shift in carbon partitioning favouring the synthesis of starch, causing an increase in the starch/sucrose ratio at the end of light period, occurred in low-light leaves. The activity of enzymes cFBAse, SPS, and AGPase, involved in the synthesis of sucrose and starch in leaves, decreased under lower light intensity, explaining the rate of accumulation of photoassimilates.


Introduction
Plant production in controlled environment agriculture (CEA) is proposed as the modern evolution of agriculture. CEA is an advanced farming technology under controlled conditions, in terms of light, temperature, carbon dioxide, relative humidity, and nutrient supply, and also in presence of an unfavourable outdoor climate and at high cultivation density [1]. It aims to guarantee high and constant year-round production, food quality and security, resilience to climate change, and sustainability, and allows high resource use efficiency and better pathogen control. In addition, CEA has the potential to meet the increasing food demand of the world population, and some applications, such as vertical farms (VF), also to shift the food production closer to urban centres.
Light influences the plant growth, development, and metabolisms, hence the yield and nutritional quality of products. Specifically, light quantity (intensity and duration) and quality (wavelength composition) affect plant behaviour throughout the entire life cycle, as plants use light as both the energy source for photosynthesis, and as a signal to activate and regulate many other fundamental processes in photomorphogenesis [2]. In absence of other limiting factors, an increase in the light intensity, as photosynthetic photon flux density (PPFD), will result in an increase of the photosynthetic rate and, likewise, of the plant yield. Hence, artificial lighting is a key factor to modulate the plant performance in protected cultivation, and the fine control of light is still one of the major technical challenges to  The total leaf chlorophyll content did not show significant difference between the lighting treatments (463.8 ± 31.8 and 501.9 ± 48.6 mg/leaf m 2 for low and high light, respectively), and the Chl a/b ratio was similar too (3.05 ± 0.05 and 2.91 ± 0.09). Table 2 shows the values of the main photosynthetic parameters recorded in plants grown under the two light intensities, after different times of exposure to lighting. Leaves of spinach plants under 200 µmol m −2 s −1 PPFD showed an assimilation rate (A) three times lower compared to those at 800 µmol m −2 s −1 (5.0 ± 0.1 and 15.4 ± 0.4 µmol CO 2 m 2 s −1 , respectively) ( Table 2). Conversely, the value of intercellular CO 2 concentration (Ci) was 13.7% higher in leaves under lower irradiance (360.3 ± 0.8 vs. 310.6 ± 2.6 µbar bar −1 at high irradiance), while stomatal conductance (g s ) was unaffected by the light intensity (470.3 ± 15.5 and 517.4 ± 35.3 mmol CO 2 m 2 s −1 for 200 PPFD and 800 PPFD, respectively) ( Table 2).

Gas Exchange
The interaction between the two factors (light intensity and times of exposure) was significant for the assimilation rate (A) and substomatal CO 2 partial pressure, while the two factors did not affect stomatal conductance (g s ) ( Table 2). The CO 2 uptake and Ci value did not change significantly during the light period, while the stomatal conductance (gs) seems to decrease after 8 h of lighting, although this difference did not result statistically significant ( Table 2).
The influence of lighting treatment on the relation between CO 2 assimilation and intercellular CO 2 concentration (A-Ci) is shown in Figure 1. Plants grown at 800 and 200 PPFD showed a similar trend of the A/Ci, proving that a higher concentration of intercellular CO 2 is not necessary to support a similar rate of photosynthesis of plants acclimated to 200 µmol m −2 s −1 compared to those acclimated to 800 µmol m −2 s −1 . Table 2 shows the values of the main photosynthetic parameters recorded in plants grown under the two light intensities, after different times of exposure to lighting.  Table 2).

Gas Exchange
The interaction between the two factors (light intensity and times of exposure) was significant for the assimilation rate (A) and substomatal CO2 partial pressure, while the two factors did not affect stomatal conductance (gs) ( Table 2). The CO2 uptake and Ci value did not change significantly during the light period, while the stomatal conductance (gs) seems to decrease after 8 h of lighting, although this difference did not result statistically significant ( Table 2).
The influence of lighting treatment on the relation between CO2 assimilation and intercellular CO2 concentration (A-Ci) is shown in Figure 1. Plants grown at 800 and 200 PPFD showed a similar trend of the A/Ci, proving that a higher concentration of intercellular CO2 is not necessary to support a similar rate of photosynthesis of plants acclimated to 200 μmol m −2 s −1 compared to those acclimated to 800 μmol m −2 s −1 .

Non-Structural Carbohydrate Content
Spinach plants grown at high light intensity showed a higher content of soluble sugars (glucose, fructose, and sucrose; Figure 2A) and total carbohydrates (soluble plus starch Figure 2B), compared to those at low-light intensity. The difference in carbohydrate content was minimal at the beginning and increased to a maximum at the end of the light period.

Non-Structural Carbohydrate Content
Spinach plants grown at high light intensity showed a higher content of soluble sugars (glucose, fructose, and sucrose; Figure 2A) and total carbohydrates (soluble plus starch Figure 2B), compared to those at low-light intensity. The difference in carbohydrate content was minimal at the beginning and increased to a maximum at the end of the light period.  Furthermore, in the same plants, the amount of sucrose accumulated in leaves at the end of the light period was about 29% greater respect to that of starch ( Figure 3C,D and Table 3). Table 3. Starch, soluble, and total carbohydrates content (μmol C as hexose equivalent·mg −1 Chl) in spinach leaves grown in a growth chamber under 800 μmol m −2 s −1 (800 PPFD) and 200 μmol m −2 s −1 Furthermore, in the same plants, the amount of sucrose accumulated in leaves at the end of the light period was about 29% greater respect to that of starch ( Figure 3C,D and Table 3). Table 3. Starch, soluble, and total carbohydrates content (µmol C as hexose equivalent·mg  Moreover, in plants at high irradiance, the concentration of glucose, fructose, sucrose, and starch increased significantly during the light period, while leaves acclimated to low light accumulated less soluble carbohydrates, and only the starch increased 20 times at the end compared to the beginning of the photoperiod ( Figure 3D).
The different carbon partitioning assimilated in leaves grown at low irradiance was underlined by the starch/sucrose ratio, which was similar between the two lighting treatments early in the light period, while it reached a value 60% lower in plants under 800 PPFD compared to those under 200 PPFD at the end of the lighting period ( Figure 4).  At the end of the light period, in leaves acclimated at low light, 68.5% of total carbohydrates were partitioned towards the formation of starch reserves, while at high irradiance 43.6% of the sugars consisted of starch. The percentage of soluble carbohydrates was two times lower than that of starch in spinach leaves grown at 200 PPFD, while soluble carbohydrates were higher than starch at 800 PPFD (Table 3).
In plants grown at 200 PPFD, the total content of carbohydrates at the beginning of the light period, after the dark phase, was about 4 times lower compared to that at high light, reflecting the decreased availability of assimilates, due to the reduction of carbon assimilation. At the beginning of a new light period, the percentage of starch reached At the end of the light period, in leaves acclimated at low light, 68.5% of total carbohydrates were partitioned towards the formation of starch reserves, while at high irradiance 43.6% of the sugars consisted of starch. The percentage of soluble carbohydrates was two times lower than that of starch in spinach leaves grown at 200 PPFD, while soluble carbohydrates were higher than starch at 800 PPFD (Table 3).
In plants grown at 200 PPFD, the total content of carbohydrates at the beginning of the light period, after the dark phase, was about 4 times lower compared to that at high light, reflecting the decreased availability of assimilates, due to the reduction of carbon assimilation. At the beginning of a new light period, the percentage of starch reached about 30% of total assimilates for both low-light and high-light spinach leaves ( Table 3).
The amount of the assimilated carbon exported from the chloroplast and accumulated as non-structural carbohydrates, in relation to the carbon assimilation rate, is shown in Table 4. Table 4. Estimated amount and export rates of assimilates in spinach leaves grown in a growth chamber under 800 µmol m −2 s −1 (800 PPFD) and 200 µmol m −2 s −1 (200 PPFD), based on data from Table 3. Assimilates exported during the day were calculated as the difference between the rate of photosynthesis and the carbon amount accumulated as total carbohydrates during the light period of 10 h (values shown in Table 3). Mean values ± S.E. (n = 12). Different letters indicate significant differences at p = 0.05 (Fisher's post-hoc test), n.s.-not significant. In spinach leaves under low and high light intensity, 390 and 1050 µmol C mg −1 chl were fixed, respectively, during the 10 h light period, considering a constant photosynthesis rate of 39 and 105 µmol CO 2 mg −1 chl h −1 , respectively. In leaves under 200 and 800 PPFD, 37.4% and 38% of the total carbon assimilated was allocated during the day as non-structural carbohydrates, respectively.

PPFD 800 PPFD
The assimilates exported during the day, calculated as the difference between the fixed CO 2 and the total carbohydrate accumulated and reported in Table 3, showed a value 2.7 times lower in leaves at 200 PPFD than 800 PPFD. The rate of assimilation during the day and night was lower for low-light leaves, and the translocation of assimilates during the night amounted to 43% and 44% of the translocation rate during the day, for low and high light leaves, respectively (Table 4).

cFBPase, SPS, and AGPase Activity
Both the maximum SPS activity (SPS max ) and AGP activity were significantly affected by light intensity (Table 5).   In spinach grown in low light conditions, SPS max and AGPase showed 36% and 20% lower values than in high light (Table 5), respectively, while cFBPase was not significantly different between the two treatments. During the light period, the activity of cFBPase and SPS max did not change significantly, while that of AGPase was 23.7% higher in the morning than in the afternoon ( Table 5). The activation state of SPS was three times higher in leaves grown in high light than in those in low light (respectively 33% and 13%). In high light growth conditions, the activation state of SPS halved its value from 4 to 8 h of light exposure (41.5% to 24.5%), while under 200 PPFD it was always lower (15.9% and 10.5% at 4 and 8 h, respectively), with no significant differences between the hours of the day. In leaves grown at 200 µmol m −2 s −1 , the AGPase/SPS max was constant in the light period, while in high light it decreased sharply, in accordance with the trend of starch/sucrose ratio ( Figure 5).

Plant Growth and Yield, and Product Characteristics and Gas Exchange
Plants can acclimate to changes in light intensity by adapting the leaf ana structure, plant morphology, and photosynthetic parameters (rate of assimilatio spiration, and stomatal conductance), which affect the biomass accumulation [20] experiment, low light intensity significantly reduced the CO2 assimilation of s plants (A), without changes in stomatal conductance (gs). This evidence, together w higher value of Ci in plants under low light, indicated that the reduced assimilati not be ascribed to stomatal limitations of the photosynthetic process [21].
The A/Ci relation in plants under both the light conditions showed a simila suggesting that spinach leaves exposed to different irradiance did not differ subst in the Rubisco activity, as demonstrated by the linear portion of the response curv ilarly, no difference was observed in the efficiency of electron transport and the r ation of Ribulose 1,5 bisphosphate (RuBP), as defined by the final part of the cur This result agrees with the evidence that Rubisco was in excess in low light plants [ that this protein does not exert a control on the photosynthetic rate for a wide r growth light intensities [24]. Moreover, the chlorophyll content and the Chl a/b ra not differ in spinach leaves under the two light regimes, indicating that the photosy

Plant Growth and Yield, and Product Characteristics and Gas Exchange
Plants can acclimate to changes in light intensity by adapting the leaf anatomical structure, plant morphology, and photosynthetic parameters (rate of assimilation, transpiration, and stomatal conductance), which affect the biomass accumulation [20]. In our experiment, low light intensity significantly reduced the CO 2 assimilation of spinach plants (A), without changes in stomatal conductance (g s ). This evidence, together with the higher value of Ci in plants under low light, indicated that the reduced assimilation cannot be ascribed to stomatal limitations of the photosynthetic process [21].
The A/Ci relation in plants under both the light conditions showed a similar trend, suggesting that spinach leaves exposed to different irradiance did not differ substantially in the Rubisco activity, as demonstrated by the linear portion of the response curve. Similarly, no difference was observed in the efficiency of electron transport and the regeneration of Ribulose 1,5 bisphosphate (RuBP), as defined by the final part of the curve [22]. This result agrees with the evidence that Rubisco was in excess in low light plants [23] and that this protein does not exert a control on the photosynthetic rate for a wide range of growth light intensities [24]. Moreover, the chlorophyll content and the Chl a/b ratio did not differ in spinach leaves under the two light regimes, indicating that the photosynthetic apparatus and its functionality were unaffected by light intensity [25]. In coffee seedlings, Rodríguez-López et al. [26] stated that biomass accumulation increased linearly with increasing light intensity, and Wu et al. [27] showed that in soybean, changes in light intensity led to considerable differences in leaf morphology and structure. In a previous experiment [28], we demonstrated that low light affected the growth of spinach, reducing the leaf thickness and plant production and increasing the shoot/root ratio. In this experiment, our data showed that in low light conditions, the leaf dry weight per plant was five times higher than the root dry weight, while this ratio was only three times in spinach plants grown in high light conditions, indicating a change in carbon allocation on the epigeal organs, as a consequence of the leaf expansion promoted to optimize the light interception.

Accumulation of Assimilates in Leaves and Translocation Rate
Plants can adjust the rate of sucrose export, as well as of starch biosynthesis and degradation to the light availability [29], and it is well known that starch formation increases in plant grown under high light intensity, due to enhanced carbon assimilation and photosynthetic product availability [30].
Sucrose is the main soluble sugar in leaves of spinach, which can be considered a sucrose accumulator species. Our data highlights this feature, as at high light plants showed a soluble carbohydrate content about 1.6 times higher than starch. However, leaves acclimated to different light conditions by modifying the partitioning of assimilated carbon during the day, as demonstrated by the daily variation of photoassimilates. Under low light intensity, in particular late in the light period, spinach leaves directed the assimilated carbon towards the starch synthesis rather than in soluble carbohydrates (68.1% vs. 31.8%), represented mainly by sucrose, in an opposite trend compared to leaves acclimated to high light (43.6% vs. 56.3%).
The leaf carbohydrate content in spinach decreased substantially during the night in both plants at 200 and 800 PPFD. Studies on source-sink relationship in Arabidopsis thaliana at the vegetative phase showed a similar trend of sugar content, with a progressive drop of carbohydrate amount, particularly during the night, related to the stunting growth in the dark and the depletion of starch reserves [31]. Spinach leaves considered in the present work were fully expanded source organs, so the starch reserves were essentially used to maintain their metabolism, and at the beginning of a new light period, low light leaves had mobilized the 51% of the accumulated starch, while high light leaves, only the 34%. It is well known that a decrease in carbohydrates availability can enhance the expression of the triose-phosphate translocators (TPT) gene, related to photosynthetic systems and to the mobilization and export of reserves, as starch that could buffer the reserve shortage [32,33].
The net assimilation rate was different for low and high light spinach leaves, so the production of photoassimilates during the 10 h of light exposure was 67% lower in leaves at 200 PPFD than at 800 PPFD. Considering these values, the total accumulation was different under low or high light intensity, resulting in a 2.7 times lower value in leaves at 200 PPFD, but in a similar percentage of total assimilates compared to 800 PPFD (37.4% and 38.0%, respectively). The average rate of assimilates utilization during the night period was lower than in the light period, for both low and high light intensity, as reported by several authors under different conditions [32,34], although the night translocation was similar, amounting to 43 and 44% under 200 and 800 PPFD, respectively, of the daily rate.
The leaf accumulation of assimilates in leaves and the translocation rate were evaluated without a direct quantification of photorespiration. However, during the light period, photorespiration was already taken into account, as the net photosynthesis corresponds to CO 2 fixation minus CO 2 release. During the dark period, the CO 2 released through the respiration process was quantified to about 2% of the assimilate utilization [32], hence, we can assume that this process has only a negligible impact on the assimilate utilization.

Activity of cFBPase, SPS, and AGP
The modulation of the carbohydrate partitioning between sucrose and starch was linked to changes in the activity of the key enzymes, cFBPase, SPS, and AGPase, that have a high control coefficient in sucrose synthesis in the cytosol (cFBPase and SPS) and starch synthesis in the chloroplast (AGPase) [10]. Reduced carbon produced by the Calvin cycle in the chloroplast is exported to the cytosol as triose phosphates and channeled to sucrose synthesis by the coordinate activity of several enzymes, with cFBPase and SPS playing a special role [35]. Alternatively, assimilates are retained in the chloroplast and used for primary starch synthesis. The flux through the two alternative photosynthetic products synthesis (sucrose and starch) are regulated by several variables, but a relevant role is played by metabolic factors (e.g., allosteric modulators), and by the genetic control of the enzyme maximum activity [35][36][37]. We have tested changes in the maximum activity of all three enzymes and the activation state of SPS [36].
The balance between starch and sucrose increases when cFBPase activity is reduced [36]. In the present work, a tendency to a reduction of cFBPase activity was observed in leaves at 200 PPFD, although the difference from leaves at 800 PPFD was not statistically significant. Strand and co-authors [38] showed that a decreased expression of cFBPase determined a switch from sucrose to starch synthesis, together with changes in metabolites relationship, such as a five to sixfold higher PGA:triose-P ratio, decreased Ru1,5bisP, and inhibition of photosynthesis. Preliminary works showed a decreased photosynthetic capacity in spinach grown in similar conditions, but at a younger growth stage, indicating that light intensity can cause a joint acclimation of photosynthesis and photosynthetic end-product synthesis capacity and fluxes [39]. In the conditions of our experiment, no sign of a decreased photosynthetic capacity was measured (similar A vs. Ci partial pressure, no change in chlorophyll content), even in the presence of a relatively higher starch and sucrose synthesis in late light time under 200 PPFD. This discrepancy is likely due to the longer acclimation period in our plants, which were older than in Battistelli et al. [39]). This evidence demonstrates the high versatility of plant acclimatory responses to light and, even more importantly, that the acclimation of starch and sucrose biosynthetic pathways in the photosynthetic cells can occur even in the absence of an evident modification of photosynthetic capacity. This point indicates that balancing photoassimilates partitioning at the photosynthetic level is of paramount importance and is a very sensitive phenomenon in response to the prevailing light environment. It also demonstrates that an efficient acclimation of photosynthate partitioning can avoid feedback inhibition of photosynthesis due to the lack of Pi recycling at the cellular level [40].
In conclusion, our work shows that in spinach leaves the acclimation to low light intensity was reached through modifications of the biomass allocation and of carbon distribution at the plant level. Spinach plants grown under low irradiance reduced biomass production and redirected the photoassimilates to leaves to optimize light interception. This change occurred without effects in the functionality of the photosynthetic process, and the reduction of photosynthetic rate was due essentially to the low radiative energy available for low light leaves. Moreover, a shift in the partitioning of carbon assimilated favouring starch synthesis was observed in leaves under low light inducing an increase in starch/sucrose ratio. Besides, the estimated synthesis and export rates of assimilates showed that the translocation during the night amounted to 43% and 44% of the rate observed in the day, for low and high light, respectively. Enzymes involved in the synthesis of sucrose and starch such as cFBAse, SPS, and AGPase lowered their activity, explaining the rate of synthesis and accumulation of photoassimilates. In terms of yield and quality, the lower light intensity decreased the crop production of spinach in a controlled environment.
Plants were supplied twice per week with a full-strength Hoagland nutrient solution [41], with a pH of 6.5 and EC 1.7 mS/cm, and with fresh water.

Sampling and Analysis
Five weeks after the expansion of cotyledons, 12 plants from each treatment were randomly collected, shoots and roots were separated, and fresh weight (FW) was measured. Dry weight (DW) was measured after oven drying at 80 • C. The dry matter percentage (DM%) was calculated as DW/FW × 100. The 4th and 5th fully expanded leaf was used to determine the content of non-structural carbohydrates (glucose, fructose, sucrose, and starch) and enzymatic activity. For non-structural carbohydrate (NSC) determination, leaf samples were collected at the end of the dark period and after 4 h, 8 h, and 10 h of light, while for enzymatic activity measurements, after 4 h and 8 h of light.

Carbohydrate Content
Measurements of non-structural carbohydrate (NSC) were performed on 1 cm 2 leaf discs, collected from leaf lamina with a cork borer, and immediately frozen in liquid nitrogen. Soluble carbohydrates were extracted at 80 • C for 45 min, after grinding the leaf discs in a glass-glass homogeniser, containing 1.5 mL of 80% (v/v) ethanol, 20% (v/v) 100 mM Hepes (pH 7.1), and 10 mM MgCl 2 . After centrifugation at 16,000× g for 5 min, the supernatant was analysed for soluble carbohydrate content. Glucose, fructose, and sucrose were determined by an enzymatic coupled spectrophotometric assay with hexokinase (1.2 U), glucose phosphate dehydrogenase (0.3 U), phosphoglucose isomerase (0.3 U), and acid invertase (30 U) as described in Moscatello et al. [43].
Starch was quantified from the pellet remaining after extraction of soluble sugars, after three washing with 50 mM NaAcetate buffer (pH 4.5), suspended in 1 mL of the same buffer, and autoclaved at 120 • C for 45 min. The suspension was incubated at 50 • C for 1 h with amyloglucosidase (40 U) and α-amylase (4 U). The glucose produced by starch hydrolysis was determined enzymatically as described above.
The SPS activity was first measured in the presence of saturating concentrations of hexose phosphates to check the enzymatic maximum activity: the extract, containing 4 µg chlorophyll, was incubated for 15 min at 20 • C with 2 mM fructose-6 phosphate (Fru-6P), 10 mM glucose-6-phosphate (Gl-6P), and 3 mM uridine diphosphoglucose (UDPG), 50 mM Hepes (pH 7.4), 4 mM MgCl 2 , and 1 mM EDTA. The SPS activity in presence of limiting conditions was measured as described above but adding 5 mM inorganic phosphate (Pi). The assay solution of 2 mM Fru-6P and 10 mM Glc-6P was used as a control. After incubation, the assay solutions were heated to 95 • C for 3 min. After this treatment, solutions were centrifuged for 2 min and an aliquot was taken to measure the UDP formed, using a dual-wavelength spectrophotometer [36].

Statistical Analysis
Statistical analysis was done by ANOVA using the STATISTICA software package (StatSoft for Windows, 1998, Tulsa, OK 74104 United States).
Growth characteristics were analysed by one-way analysis of variance (ANOVA). Data on carbohydrate contents and enzymatic activity were analysed using two-way ANOVA, with the time of sampling and light intensity as factors. Differences between averages were tested by Fisher's post-hoc test, with a significance level of p = 0.05 and designated with different letters.

Conclusions
We produced new evidence on how controlled growth conditions can affect plant growth, photosynthesis, and carbohydrate amount and partitioned it at both the leaf and plant level. Modifications of photosynthesis and growth in response to light environment are key aspects in indoor plant cultivation, dramatically impacting the energy conversion efficiency, productivity, and produce quality [39]. Furthermore, soluble carbohydrates in leaves are a key determinant of the taste of vegetables, and total carbohydrates at harvest might influence the cell survival during post-harvest handling and commercialization, thereby affecting the product shelf-life and consumer acceptance. Fully controlled environment agriculture can result in energetic and economical sustainability by better knowing the biochemical and physiological determinants of plant response to the growth environment.