An Update of the Cenchrinae (Poaceae, Panicoideae, Paniceae) and a New Genus for the Subtribe to Clarify the Dubious Position of a Species of Panicum L.

Subtribe Cenchrinae, so-called as the “bristle clade”, is a monophyletic group of panicoid grasses characterized by having sterile branches or bristles on the inflorescences in most of its species. Within this subtribe is also placed Panicum antidotale Retz., an “incertae sedis” species of Panicum L. which lacks bristles along the inflorescence. In this study, we present an update of the subtribe Cenchrinae based on molecular, morphological, and anatomical evidence to clarify the systematic position of P. antidotale in the Cenchrinae, excluding it from Panicum and establishing it in a new genus (i.e., Janochloa Zuloaga & Delfini); the morphological features distinguishing the new genus from other closely related taxa are properly discussed and an identification key to the 24 genera recognized within Cenchrinae is presented. We also add American Setaria species, not tested before, of subgenera Paurochaetium and Reverchoniae, discussing the position of these taxa in actual phylogeny of the genus as well as defining placements in the tree of Setaria species that were imprecisely located in previous analyses. A comparison with the results from other studies, comments on Stenotaphrum Trin. and a brief discussion on conflicting placements in Cenchrus and related taxa, and of Acritochaete Pilg. are also included.


Introduction
Subtribe Cenchrinae Dumort., of the Paniceae R. Br., consists of 24 genera morphologically defined by the presence of sterile branches or bristles on the inflorescence in the majority of the species; this subtribe is a sister to subtribe Melinidinae, and both form a monophyletic group with subtribe Panicinae that is referred to as the MPC clade, wellsupported in diverse phylogenies based on chloroplast, mitochondrial, and nuclear rDNA gene sequence [1][2][3][4][5]. Species of the Cenchrinae are also homogeneous, in terms of photosynthesis, because all genera and species are of the C 4 NADP-me subtype (see comments on Acritochaete Pilg. below); on the other hand, taxa of subtribe Melinidinae are of the PCK subtype, while all Panicinae are of the NAD-me subtype [6][7][8]. Although monophyly of subtribe Cenchrinae was consistent in all phylogenetic studies [1,5,9], resolution of basal clades is low; therefore, with the relationship of genera, in many cases, still doubtful.
Most genera of the subtribe have in common bristles, from one to many, and persistent to deciduous, on the inflorescence. However, there are four genera that lack bristles along the inflorescence, i.e., 1. Whiteochloa C.E. Hubb., with six species in Australia and the Pacific Islands; the genus was originally classified in the Panicinae [2] but later confirmed in the Cenchrinae [10]; 2. Alexfloydia B.K. Simon, with one species restricted to southeastern in-depth research. It is clearly not a natural group, but more evidence is still needed to allow restructuring of the taxonomy of Setaria. For this reason, our purpose here is to give more information about species of the genus, and discuss the results, without pursuing a solution to its intricate phylogeny.
The aim of this study was to increase the knowledge of taxa of the Cenchrinae through: (a) solving the systematic position of Panicum antidotale in the Cenchrinae, excluding the species from Panicum and establishing a new genus; (b) adding American Setaria species, not tested before, of subgenera Paurochaetium and Reverchoniae, discussing the position of these taxa in actual phylogeny of the genus; (c) analysing Old World species of Setaria and other genera of Cenchrinae from Genbank not considered in previous phylogenies [29]; and (d) presenting new accessions of some other New World species of Setaria (not Paurochaetium and Reverchoniae), in order to confirm their placement in the phylogenetic tree, and more vouchers of some species that were imprecisely located in [29], as they were represented by partial, not fully double-stranded and/or poor-quality accessions. In addition, we are including a new key to recognize all genera of the Cenchrinae.

Results
The aligned data matrix for 180 accessions consists of 2077 nucleotide positions, of which 278 characters were phylogenetically informative. The parsimony analyses found 80 trees of 776 steps (uninformative characters excluded), with a consistency index (CI) of 0.466 and a retention index (RI) of 0. 804. The strict consensus tree from MP, the Maximum Clade Credibility Tree (MCCT) from BI and the ML tree produced similar topologies showing the same strongly supported clades; thus, only the MCCT from BI is presented here along with branch supports obtained under MP and ML analyses ( Figure 1). The aligned data matrix and trees from the three methods of analysis are available at Repositorio Institucional CONICET Digital under the following link: http://hdl.handle.net/11336/16 3438 (accessed on 28 July 2022).
For eight taxa, we were able to sequence a second voucher (i.e., Alexfloydia repens B.K. Simon Table S1 available in  Supplementary Material here]. Except for S. nicorae, whose mutations in the sequences led its accessions to distinct placements (i.e., separated into two clades), the two accessions of the same species had identical or nearly identical sequences and were placed together by the three analyses ( Figure 1).
Based on the phylogenetic evidence presented here, the subtribe Cenchrinae, as currently circumscribed, is paraphyletic since Acritochaete volkensii Pilg. was placed outside the "bristle clade", unrelated to the remaining genera of the subtribe. Although the two accessions of Panicum antidotale were consistently placed together (Bayesian posterior probability (BPP) 1/ML bootstrap (MLB) 100/parsimony bootstrap (PB) 100) embedded in the Cenchrinae, its position in the tree is unclear. In the Bayesian analysis Panicum antidotale formed a weakly supported clade (BPP < 0.50) with the two accessions of Alexfloydia repens, Xerochloa barbata R. Br., and Xerochloa laniflora Benth. (Figure 1), while the two bootstrap analyses recovered it as a sister to Paratheria prostrata Griseb, a relationship also with almost no support (MLB and PB < 50).
Our phylogeny showed the Setaria species/accessions analyzed distributed in different clades through the tree (A-E), being Setaria magna Griseb. and Setaria rara (R. Br.) R.D. Webster in an uncertain position. Species of subgenera Paurochaetium and Reverchoniae (see Supporting Information Table S2) were placed into two clades and two subclades (A-D; Figure 1), as detailed next.
Our phylogeny showed the Setaria species/accessions analyzed distributed in different clades through the tree (A-E), being Setaria magna Griseb. and Setaria rara (R. Br.) R.D. Webster in an uncertain position. Species of subgenera Paurochaetium and Reverchoniae (see Supporting Information Table S2) were placed into two clades and two subclades (A-D; Figure 1), as detailed next.  1/MLB 94/PB 100) are strongly (BI) supported as sister group to all clade A species. Clade A was also resolved in a weakly (BI) supported position sister to clade B, which groups most of the Setaria species from the Americas. The remainder species of the subgenera Paurochaetium and Reverchoniae (i.e., Setaria distantiflora (A. Rich.) Pilg., Setaria leonis (Ekman ex Hitchc.) León, Setaria reverchonii (Vasey) Pilg., S. utowanaea, and S. variifolia; Table S2) were placed within clade B but they do not appear to be related to each other, except for the two accessions of S. utowanaea (BPP 1/MLB 100/PB 100), strongly related to S. distantiflora in subclade C (BPP 1/MLB 98/PB 96). The two accessions of S. variifolia were consistently placed together (BPP 1/MLB 99/PB 100) and supported as a sister group to all clade B species. Within subclade D, the two accessions of Setaria cernua Kunth (BPP 1/MLB 95/PB 100) are closely related to S. reverchonii with a strong branch support in the BI (BPP 0.98/MLB 55/PB < 50). Bayesian analysis also found weak support (BPP < 0.50) for a sister relationship between S. leonis and S. scheelei, but this grouping was not supported in ML and MP.
The two accessions of Setaria nicorae Pensiero are also included within the larger American clade (clade B), but unrelated to each other. Setaria hunzikeri Anton is nested with one accession of Setaria lachnea (Nees) Kunth in Bayesian and ML analyses (BPP 0. 86 Setaria magna and S. rara are the only two Setaria species that have not been consistently assigned/related to any of the retrieved clades. Although their two accessions were unambiguously supported together (BPP 1/MLB 100/PB 100; BPP 0.99/MLB 92/PB 100, respectively), their placements in this phylogeny remain unclear. Both species were poorly BI and ML supported as successive sisters to four temperate Asian species (i.e., Setaria faberi R.A.W. Herrm., Setaria italica (L.) P. Beauv

Monophyly of the Cenchrinae
The monotypic Acritochaete volkensii Pilg. is an annual species found in shady forests of tropical Africa [32,33], currently treated within the subtribe Cenchrinae [14]. It has inflorescences bearing persistent setae, a character state shared with the "bristle clade"; however, our results placed A. volkensii outside the Cenchrinae, as also indicated in [3,5]. Despite the morphological similarities with Cenchrinae, the photosynthetic C 3 pathway of A. volkensii is absent within this subtribe, which includes only C 4 NADP-me plants [1,14]. According to [3], Acritochaete Pilg. is closely related to members of the Boivinellinae Pilg. subtribe known as "the forest shade clade" [3,34], and which groups mostly physiologically C 3 genera [1,14]. Thus, for both subtribes to be monophyletic, A. volkensii must be recognized within the subtribe Boivinellinae.
Although these studies represent an important advancement in the evolutionary knowledge of the Panicoideae, there are still "incertae sedis" species of Panicum in need of a taxonomic definition. Among these taxa, [14] included 18 non Kranz species in the "Sacciolepis grex", 12 non Kranz species in the Boivinellinae, 3 Kranz PEP-ck species in the Melinidinae, and one Kranz NADP-me species in the Cenchrinae. The single "incertae sedis" species of Panicum within the Cenchrinae is P. antidotale, introduced all over the world due to its medicinal, environmental and animal food uses, being the native range from Sinai to Indo-China [33]. The species is characterized by having a perennial habit with strong rhizomes, rigid and solid internodes, lax panicles, glabrous spikelets, the lower glume 1 /2 or more the length of the spikelet, and upper glume and lower lemma 5-9-nerved [21]. As previously stated, P. antidotale is the only remaining C 4 "Panicum" species of the MS type (XyMS-) that has a carbon isotope value of −14.1 and which anatomically has a single mestome Kranz sheath around the vascular bundles, centrifugal arrangement of chloroplasts, and three mesophyll cells between vascular bundles [57,58]; these characteristics obviously imply that the species must be segregated from Panicum s. str.
The placement of P. antidotale in the "bristle clade" was first indicated by [39] basing on the chloroplast ndhF data and using a sequence obtained from a single correctly identified accession. By the addition of a second voucher specimen and both accessions consistently placed together, our data confirmed that P. antidotale is related to members of subtribe Cenchrinae, although its position in the tree is unresolved.
As in other three genera of Cenchrinae (i.e., Alexfloydia B.K. Simon, Whiteochloa C.E. Hubb. and Zuloagaea), P. antidotale has spikelets and inflorescences morphologically similar to those of Panicum s. str. and lacks setae [1]. Alexfloydia, a monotypic genus from Australia, is a stoloniferous perennial, with decumbent culms, branching at the lower nodes, with ciliate ligules, terminal inflorescences, and few flowered, spikelets laterally compressed. Spikelets are adaxial; the lower glume is 5-7-nerved, 3 /4 the length of the spikelet; the upper glume and lower lemma are 9-nerved, the lower flower is male, and the upper anthecium is cartilaginous and smooth [59]. Whiteochloa C.E. Hubb., with six species distributed in Australia, New Guinea, and neighboring islands, includes annual or perennial plants, with shortly rhizomatous perennial; its internodes are hollow, its ligules membranous-ciliate to ciliate, and its inflorescence a panicle composed of unilateral racemes, laterally-compressed spikelets. The lower glume is 3-5-nerved, and the upper glume and lower lemma are 5-7-nerved; the lower flower is male, and the upper anthecium is stipitate, indurate and rugose [60]. Zuloagaea is also a monotypic genus growing from United States to northern South America, Colombia to Ecuador, characterized by its corm-like base, membranous ligules, open and pyramidal inflorescences, dorsiventrally compressed spikelets, 3-nerved, lower glume, 1 ⅔ the length of the spikelet, the upper glume and lower lemma are subequal and 5-nerved; the lower palea is hyaline, the lower flower is male or absent, and the upper anthecium is not stipitate; rugose and indurate [56].
Despite its divergent morphology, P. antidotale shares with the remainder Cenchrinae a Kranz leaf blade anatomy typical of the NADP-me subtype photosynthetic pathway [10,57,58]. Ref. [56] hypothesized the possibility that Zuloagaea bulbosa has cryptic setae that would link it to the more closely related species of Setaria and Ixophorus, which, perhaps, could also have occurred in P. antidotale, Alexfloydia repens, and in Whiteochloa; developmental patterns of inflorescences in these species should be studied to determine the basis for the absence of bristles [1].
Based on previous studies [1,14,30,39] and the evidence presented here, we believe that "Panicum antidotale" needs to be treated as a monospecific genus within the Cenchrinae in order to take into account the evolutionary history of the subtribe as currently known and to provide a means of reference to facilitate communication about these taxa. Monotypic taxa are a challenging group that deserve attention [61]. Even when we acknowledge that the erection of monotypic genera is a controversial matter (e.g., [62,63]), here we propose to recognize one new monotypic genus in Paniceae, subtribe Cenchrinae, a decision supported by both morphological and molecular data. The erection of Janochloa Zuloaga & Delfini increase the total number of genera in the Cenchrinae to 24, once Acritochaete is excluded (see previous comments on item 3.1). This subtribe comprehends 12 monotypic genera, seven of them endemic to Australia (i.e., Alexfloydia, Chamaeraphis R. Br Long-rhizomatous perennial, with decumbent culms and solid, glaucous internodes. Ligules membranous-ciliate; blades lanceolate. Inflorescence a lax and a diffuse panicle. Spikelets long ovoid, chasmogamous and glabrous. Lower glume 3 /4 as long as the spikelet, 3-5-nerved. Upper glume and lower lemma subequal and 5-9-nerved. Lower palea elliptic and glabrous; lower flower male or absent. Upper anthecium ovoid, glabrous, and indurate. Caryopsis ovoid; hilum punctiform, embryo 1 /2 or more the length of the caryopsis.

Setaria P. Beauv.
Setaria is currently recognized as a difficult and non-monophyletic genus with the species isolated or distributed in four blocks of clades or groups of clades within the Cenchrinae [15,29]. These clades are correlated and named according to their geographic origins (i.e., 1. Africa, tropical-Asia, 2. Australia, Australasia, 3. temperate Asia, and 4. the Americas) and none of the tested subgenera (i.e., Ptychophyllum and Setaria) were retrieved as monophyletic [15,29].
In our preliminary phylogeny, relationships among Setaria species were similar to those shown in previous ndhF-based phylogenies [15,29] with notable differences mainly in the composition of the proposed American clades. Here, most of the Setaria species from the Americas were resolved in two main clades, one major (clade B, corresponding to clade X of [29]) and one minor (clade E, corresponding to clade II of [29]), both morphologically quite distinct. The major clade was originally composed of South American perennial species; however, due to the placement of some taxa of the subgenera Paurochaetium and Reverchoniae [see Supporting Information Table S2] within this clade, its range was extended to Central and North America. Our findings also identified a clade related to the morphology of the species rather than its geographic origins (i.e., clade A), which groups S. chapmanii, a taxon previously treated in subgenus Paurochaetium [20] (Figure 1).
As expected, the subgenera Paurochaetium and Reverchoniae (Table S2) are non-monophyletic like the other subgenera of Setaria. Although they share morphological similarities, five of the six species analyzed (i.e., S. distantiflora, S. leonis, S. reverchonii, S. variifolia, and S. utowanaea) were resolved within the major American clade (clade B), according to their geographic origins. On the other hand, S. chapmanii, also an American species, was unambiguously strongly supported within clade A, related to other species with inflorescences "Paspalidium type".
As mentioned earlier, the taxonomic history of subgenera Paurochaetium and Reverchoniae are linked to that of the genus Paspalidium [16,25,27,28,74]. Species of subgenus Paurochaetium were originally described as a subgenus of Panicum [75] to accommodate taxa in which setae are present only at the ends of the primary branches of the inflorescence. Subgenus Paurochaetium was first placed under Setaria at the rank of section by [76] and elevated to subgenus by [20]. Following [75]'s concept, [25] established the genus Paspalidium, segregating it from Setaria, but species of subgenus Paurochaetium were transferred to Paspalidium only decades later [74]. As the circumscriptions of the two genera overlap and the distinction between them is somewhat arbitrary, the Paspalidium species were transferred back to Setaria [16,27,28] (see Table S2 for a synopsis of species and different classifications for the taxa of subgenera Paurochaetium and Reverchoniae), a result supported by molecular analyses [1,15,29].
Subgenera Paurochaetium (five accepted species) and Reverchoniae (two accepted species) (Table S2) [18,26] include caespitose perennial plants distributed from the United States (New Mexico, West South Central, and Florida) to northern South America (Venezuela and Colombia), being mostly concentrated in the Caribbean [18,20,26,33,74]. Setaria chapmanii, analyzed here for the first time, grows on limestone, coral, shell or sandy soils in the Florida Keys, the Bahamas, Cuba, and the Yucatan Peninsula [20]; its panicles have branches with spikelets biseriate, the blunt first glume turned away from the rachis and the back of the upper lemma toward it, and a single bristle present below the terminal spikelets [74]. Although this species was previously treated within subgenus Paurochaetium [20], the well-ordered arrangement of its spikelets in unilateral spikes is highly anomalous in this group [74], as well as the lack of the lower palea [20]. In our phylogeny, S. chapmanii is placed in clade A and turned out to be the only species of subgenus Paurochaetium that is related to the others previously considered in Paspalidium (the remaining species of subgenus Paurochaetium were included in clade B).
Clade A groups species with inflorescences "Paspalidium type" related to wet/aquatic habitats and, except for S. chapmanii, which have slender culms, its members are characterized by having spongy culms [17,74,77]. The relationship between S. chapmanii and S. geminata for sharing the same type of habitat has been previously highlighted [74]. Setaria geminata is native to Africa and Asia, introduced unintentionally in tropical and subtropical areas of other continents [17]. It is an aquatic species with thick and spongy culms, while S. chapmanii inhabits temporary pools and marshes and is characterized by having culms mostly simple, erect, slender, and smooth [74,77]. The spongy culms of S. geminata are also shared with S. punctata and S. uda. From the former species, S. geminata is distinguished by having spikelets ovoid and lower palea well-developed while in S. punctata the spikelets are ellipsoid and lack lower palea [17]. Setaria uda is a species native to Australia and Papua New Guinea [17], and its position within clade A is confirmed here by the addition of a second voucher (i.e., S. uda 2). It differs from S. geminata and S. punctata mainly by having a caespitose habit; it lacks rhizomes and has smaller spikelets [17].
In ; these relationships were not retrieved by our analyses with exception of Plagiosetum refractum, whose sister position to the "Australian Paspalidium clade" is poorly supported by BI and ML. Setaria rara is endemic to Australia, commonly found in arid areas associated with creeks or lagoons [17]. It was previously included in Paspalidium and shares an annual habit with S. basiclada [17]; however, the position of S. rara remains unresolved even with the addition of a second voucher. Setaria magna is also an annual species but is native to tropical and subtropical Americas and morphologically different from Paspalidium. It is distinguished from other Setaria species by its robust aspect with culms as much as 4 m tall and ligules forming an inverted "V" [18]. Although the placement of S. magna is not yet defined, our analyses corroborated that S. magna is not phylogenetically related to species of the American clades.
Clade B groups most of the American perennial species of Setaria, and as in previous studies [15,29], it was retrieved in all analyses. Setaria cernua, whose position was unclear in [29], was consistently supported within this clade, nested with S. reverchonii. Setaria cernua is characterized by having conspicuous superficial rhizomes, tillers with strongly keeled leaves resembling those of some Iridaceae, lower anthecium male with developed anthers, and upper anthecium shorter than the spikelet [18]. This unique combination of characters states led [18] to establish the monotypic subgenus Cernatum Pensiero, which was not supported by our findings, and disagrees with previous results [29] which had recovered it in an isolated position.
The two species of subgenus Reverchoniae (Table S2) were also placed in clade B, but they do not appear to be related to each other nor to the subgenus Paurochaetium taxa. Subgenus Reverchoniae was raised to accommodate species with panicles erect, spikelets randomly disposed on the branches, and the central inflorescence axis scabrous [26]. Setaria variifolia differs from S. reverchonii mainly in having the lower palea well-developed and by the geographic distribution in the Yucatán peninsula of Mexico and Mesoamerica (vs. lower palea absent or rudimentary and the geographically distributed in Texas, New Mexico, and Oklahoma (United States) and northern Mexico in S. reverchonii) [26]; the placement of S. variifolia within the larger American clade is confirmed here by sequencing of two vouchers but its relationships remain unknown.
Setaria scheelei has been assigned to subgenus Setaria [20] and was included in our analysis because it shares a geographic distribution pattern similar to that of subgenus Paurochaetium (i.e., native to southwest and south-central United States to Mexico). Setaria scheelei is a highly polymorphic species characterized by having a robust aspect; culms usually geniculate at the base, blades are usually flat and pubescent, and the upper lemma short-apiculate, incurved, and finely cross-wrinkled [20]. A sister relationship between S. leonis and S. scheelei was weakly supported in the Bayesian analysis but these two species are quite morphologically distinct and do not share ecological similarities. Setaria leonis has a more delicate aspect (30-60 cm tall), slender culms, erect or spreading, leaf blades broad but narrower than the sheath at the base, and a muticous upper lemma; it is commonly found in shad places on alluvial soils of limestone canyons and river bottoms while S. scheelei prefers open areas, rocky slopes, and clearings [20]. Setaria leonis shares the slender culms, geographic distribution, and habitat with Setaria pradana (León ex Hitchc.) León [20]; however, we were not able to analyze the latter species because the ndhF failed in all amplification attempts.
Setaria hunzikeri, here analyzed for the first time, was resolved as sister to one accession of S. lachnea, also within clade B. The two species are important forage grasses native in South America and are morphologically very similar [18], so their close relationship in our analyses is not surprising. Setaria hunzikeri differs from S. lachnea by having hirsute and narrower blades and smaller inflorescences up to 8 cm long (vs. glabrous or scabrous blades and inflorescences ranging from 7 to 25 cm long) [18].
Setaria nicorae was represented in [29]'s phylogeny by a partial sequence and placed in a polytomy together with other South American perennial species. Here, by including a second voucher with a complete ndhF sequence, we confirmed the placement of S. nicorae within the major American clade, but its relationships remain unknown. Morphological similarities between S. nicorae and S. utowanaea have been noted by [18], mainly because they share the caespitose habit with conspicuous rhizomes, ovoid spikelets, and 5-7-nerved upper glume; however, these two species are not phylogenetically related. Setaria utowanaea is sister to S. distantiflora, and both species are morphologically distinct but similar in general aspect and share the habitat (i.e., open areas and rocky soils). Setaria distantiflora is endemic to the Caribbean and is characterized by having a caespitose habit, lacking conspicuous rhizomes, ligules as a fringe of very short hairs, and lanceolate-ellipsoid spikelets while S. utowanaea has short rhizomes, membranous-ciliate ligules, ovoid spikelets and is more widely distributed (i.e., Caribbean, Colombia to Venezuela) [18,20].
As presented by [29], the minor American clade (clade E) groups annual species with "bottle-brush inflorescences" (i.e., cylindrical, dense, and continuous spiciform panicles), and both antrorse and retrorse prickles on the same bristle, the latter indicated as potential morphological synapomorphy of this clade [29]. In this phylogeny, bootstrap supports for relationships of clade E are weaker than that retrieved previously, possibly because of the placement of S. longipila, here analyzed for the first time, within this clade. Setaria longipila is also an annual species, but its subspiciform panicles [20] are distinctive. On the other hand, Setaria grisebachii E. Fourn., another annual species with inflorescences similar to those of S. longipila, is sister to Setariopsis auriculata (E. Fourn.) Scribn., and are both related to Zuloagaea bulbosa (Kunth) E. Bess. The position of S. grisebachii outside clade E will have to be verified by inclusion of multiple accessions and other morphologically similar American species (e.g., Setaria liebmanni E. Fourn.) in further analyses.

Cenchrus and Related Taxa
Cenchrus L. is a cosmopolitan genus with approximately 120 species [5] characterized by having one or several spikelets accompanied by one bristle or surrounded by an involucre of multiple bristles, or with bristles fused in a cup-like structure [13]. It is a monophyletic genus only when Cenchropsis, Pennisetum, Kikuyuochloa, the monotypic Odontelytrum, and Snowdenia are included within it [1,[12][13][14]; however, recent molecular phylogenetic studies [3] and our findings showed Cenchrus paraphyletic with Stereochlaena cameronii embedded in the "Cenchrus clade". Stereochlaena cameronii is morphologically quite distinct from Cenchrus in having digitate racemes, imbricate paired spikelets, and lower lemma awned [24]. Therefore, to reach any decision on the inclusion of this species within Cenchrus, its placement in the tree must be confirmed by additional accessions and more variable markers.
Pseudochaetochloa australiensis Hitchc., an endemic species to Australia, is considered as a synonym of Cenchrus arnhemicus (F. Muell.) Morrone in [33]; however, this treatment is not supported by our analyses. Here, P. australiensis formed a strongly supported clade with the dioecious Australian Spinifex and Zygochloa, which corroborates its classification as an independent genus of Cenchrus. Pseudochaetochloa australiensis is distinguished from these two by having monoecious two-flowered spikelets bearing a single bristle subtending many of the spikelets, lower anthecium well developed, and both lemmas membranous, similar in size, shape, and texture [78].

Stenotaphrum Trin.
Stenotaphrum is a primarily tropical genus including seven species [33,79,80] and, as in Paspalidium, its secondary-order inflorescence branches end in a bristle [80]. The placement of Stenotaphrum within subtribe Cenchrinae and its phylogenetic relationships have been uncertain due to limited data from previous studies (i.e., in [29] it was represented only by Stenotaphrum secundatum (Walter) Kuntze). Here, by increasing the number of species sampled (total of four), our results corroborated the close relationship of Stenotaphrum with Setaria retiglumis (syn. Paspalidium retiglume (Domin) Hughes) and Uranthoecium truncatum, retrieving it as a paraphyletic genus, as suggested in [3]. Stenotaphrum is distinguished from Setaria and the monotypic Uranthoecium by having the main inflorescences axis thickened and flattened, with the secondary branches embedded in it [79,80]. Uranthoecium truncatum is characterized by having short lateral branches, disarticulating rachis and truncate glumes, a set of features unique in this clade. As in previous analyses [29], here U. truncatum was also strongly (BI and MP) supported as sister to S. retiglumis. Both are caespitose annual species endemic to Australia and exhibit a very similar foliar anatomy [1,31], although S. retiglumis resembles other Setaria species in the phenotype.

Taxon Sampling
The aligned data matrix used in the phylogenetic analyses includes a total of 180 accessions, of which 172 are ingroup corresponding to 22 (of 24) genera of the subtribe Cenchrinae [see Supporting Information Table S1]. The chloroplast DNA (cpDNA) ndhF matrix previously published [29], excluding the outgroup, was completed with 63 new sequences. Of these, we have sequenced 20 new accessions corresponding to species of the subgenera Paurochaetium and Reverchoniae of Setaria (Table S1, indicated with *) including those without a defined placement in [29] (Table S1, indicated with **) plus a second accession of Alexfloydia repens and Panicum antidotale. Eight species belonging to six closely related genera were selected as outgroup, based on [1,29]: Aakia (Paspaleae, Paspalinae), Eriochloa Kunth, Moorochloa Veldkamp, Rupichloa Salariato & Morrone, Urochloa P. Beauv. (Paniceae, Melinidinae) and Panicum (Paniceae, Panicinae). Information about vouchers and accession numbers of the new sequences obtained for this study and those available in GenBank are given in Table S1.

DNA Amplification and Sequencing
Total genomic DNA was extracted from herbarium material using modified CTAB protocols from [81]. For the species that failed this protocol, the DNA was isolated using the DNeasy Plant Mini Kit (Qiagen, Hilden, Germany), following the manufacturer's recommendations. Each species was amplified from a single voucher specimen, but a second voucher was also included for some taxa. The ndhF gene, coding NADH dehydrogenase subunit F, was amplified by polymerase chain reaction (PCR) and sequenced for each taxon. The complete region was amplified with a battery of primers in different combinations in four overlapping fragments using primer pairs specified by [39,82]: 5F-536R, 536F-972R, 972F-1666R, and 1666F-3R. Due to a lot of samples with a difficult amplification of the region 1666F-3R, a new reverse primer near the 3R region was designed for PCR amplification and sequencing of ndhF within the subfamily Panicoideae: 2150R

Phylogenetic Analyses
Sequence editing and assembly were performed with MEGA v. 7.0 [83]. Accuracy of sequences was assessed by visual inspection of the chromatograms. Alignments were generated with Clustal X v. 2 [84] under the default settings and were trimmed to remove part of the 3 end, for which many sequences were incomplete. The alignments obtained were then checked and improved manually, when necessary, by visual refinement using the program MEGA v. 7.0 [83]. The phylogenetic reconstruction was based on parsimony (MP) [85], maximum likelihood (ML) [86,87], and Bayesian inference (BI) [88] methods. In all analyses, gaps were considered as missing data. To determine the best-fitting nucleotide substitution model, data were submitted to jModeltest 2. 1.1 [89] and the Akaike information criterion (AIC) selected TVM+I+G.
Parsimony analyses were performed using TNT ver. 1.1 [90] with Fitch parsimony [85] as the optimality criterion. All characters were equally weighted and treated as unordered. A heuristic search was conducted using 1000 random taxon-addition replicates, with the tree-bisection-reconnection (TBR) algorithm, saving up to 15 trees per replicate to prevent extensive swapping on islands with many trees. The resulting trees were then used as starting trees for a second-round search using TBR branch swapping with an upper limit of 10,000 trees. Nonparametric bootstrap support (BS) was estimated using 10,000 pseudo-replicates, and the same parameters were used in our MP analyses [91]. Bootstrap percentages of 50 to 80 were considered weak, 81 to 90 moderate, and >90 strong.
ML analyses were conducted using RAxML-HPC2 on XSEDE (v. 8.2.12) [92] in the Cyberinfrastructure for Phylogenetic Research (CIPRES) Portal v. 3.3 [93]. For these analyses we used the implemented algorithm, which allows one to perform optimal tree searches and obtain bootstrap support [91] in one single analysis [94]. To this end, we performed 1000 bootstrap replicates with a subsequent search of the maximum likelihood tree, using the GTRGAMMA nucleotide substitution model [92], individual per-site substitution rates (-c), and default setting of likelihood acceptance (-e), 25 and 0.1, respectively. Bootstrap percentages of 50 to 80 were considered weak, 81 to 90 moderate, and > 90 strong.
Bayesian analyses were performing using MrBayes v. 3.2.7a [95] in the CIPRES Portal [93] with nst = 6 and rates = invgamma. The datasets were analyzed in two independent runs of 10 million generations, each with four Markov chains (one cold and three heated chains), sampling every 1000 generations. Convergence and effective sample size (ESS) of the runs were assessed in Tracer v. 1.7 [96], checking that ESS > 200 for all parameters. After discarding the initial 2500 trees of each run as burn-in (25%), the remaining trees (15,002) were summarized in a Maximum Clade Credibility Tree (MCCT) including the posterior probabilities (BPP) as branch support estimates. The cutoff for strong support in the Bayesian analyses was 0.95 (roughly equal to p < 0.05) posterior probabilities and values below 0.8 were considered not supported.

Taxonomic Treatment
The taxonomic treatment was based on bibliographical research including original descriptions, as well as analyses of specimens housed in the following herbaria (acronyms according to [97]): ANSM, BRI, CANB, COL, F, IAN, MEXU, MO, NT, NY, ONH, P, SI, and US. We examined types in person and images available online at the JSTOR Global Plants website (http://plants. jstor.org, accessed on 10 October 2022) and/or at the websites of the aforementioned herbaria. The protologues of all taxa have been checked. For each name, the place of valid publication is given followed by the holotype or lectotype. Accepted names are in bold italic. Complete synonymy was provided and cited in chronological order.

Conclusions
This study allowed us to present the morphological features of 24 genera of subtribe Cenchrinae of the Paniceae, including the geographic distribution of taxa and a key to distinguish them. Among the results, the genus Acritochaete was excluded from the subtribe, new taxa, of subgenera Paurochaetium and Reverchoniae were analyzed within the polyphyletic genus Setaria, relationships of Cenchrus with other genera, such as Stereochlaena and Pseudochaetochloa are also discussed as well as comments on the genus Stenotaphrum. Finally, the new monotypic genus Janochloa, segregated from Panicum, is phylogenetically analyzed, described, and fully illustrated.
Supplementary Materials: The following supporting information can be downloaded at: https: //www.mdpi.com/article/10.3390/plants12040749/s1, Table S1: Taxa, voucher information, and GenBank accession numbers for ndhF sequences. Species of the subgenera Paurochaetium and Reverchoniae of Setaria P. Beauv. and sequences obtained for this study are indicated with an asterisk (*); species without a defined placement in [29] are indicated with double asterisk (**). Accepted names