Salt-Induced Modulation of Ion Transport and PSII Photoprotection Determine the Salinity Tolerance of Amphidiploid Brassicas

Brassica species show varying levels of resistance to salt stress. To understand the genetics underlying these differential stress tolerance patterns in Brassicas, we exposed two widely cultivated amphidiploid Brassica species having different genomes, Brassica juncea (AABB, n = 18) and Brassica napus (AACC, n = 19), to elevated levels of NaCl concentration (300 mM, half the salinity of seawater). B. juncea produced more biomass, an increased chlorophyll content, and fewer accumulated sodium (Na+) and chloride (Cl−) ions in its photosynthesizing tissues. Chlorophyll fluorescence assays revealed that the reaction centers of PSII of B. juncea were more photoprotected and hence more active than those of B. napus under NaCl stress, which, in turn, resulted in a better PSII quantum efficiency, better utilization of photochemical energy with significantly reduced energy loss, and higher electron transport rates, even under stressful conditions. The expression of key genes responsible for salt tolerance (NHX1 and AVP1, which are nuclear-encoded) and photosynthesis (psbA, psaA, petB, and rbcL, which are chloroplast-encoded) were monitored for their genetic differences underlying stress tolerance. Under NaCl stress, the expression of NHX1, D1, and Rubisco increased several folds in B. juncea plants compared to B. napus, highlighting differences in genetics between these two Brassicas. The higher photosynthetic potential under stress suggests that B. juncea is a promising candidate for genetic modifications and its cultivation on marginal lands.


Introduction
Salinity is one of the significant detrimental factors reducing crop cultivation, affecting over one billion hectares of the world s farmland [1]. The accumulation of high salt concentrations in different plant parts severely limits crop yields, especially in arid and semi-arid areas. Excessive ion uptake, sodium (Na + ), and chloride (Cl − ), along with a reduction in water, induce cytotoxicity and nutritional imbalance, which affect plant growth and development [2]. The uptake of toxic salt ions affects plant growth in two back-toback phases. The first "osmotic phase" decreases plant growth through a declined rate of soil water potential, while the second "ionic phase" arises as the old leaves begin to die when salt ions exceed the vacuole capacity and start to accumulate in the cytoplasm [3]. The retention of salt ions in the roots, serving to limit their transfer towards the upper parts, is also a mechanism for plant survival under salt stress [4]. Another stress survival week-old plants of Brassica juncea (black bars) and Brassica napus (grey bars) were exposed to either 0 or 300 mM NaCl. Different growth parameters, such as plant height (a), leaf area (b), shoot dry weight (c), and root dry weight (d), were determined after 15 days of the treatment. Data points represent the means ± SD of three biological replicates. Significant differences are shown with different asterisks (***, **, * at p = 0.001, 0.01, 0.05, respectively).

Effects of Salinity on Pigments and Ion Concentration
Plants of B. napus and B. juncea were grown in pots and exposed to 300 mM NaCl stress. The concentrations of photosynthetic pigments such as chlorophyll and carotenoids and the chlorophyll a to chlorophyll b ratio were determined. Under control conditions, all these parameters were higher in B. napus than in B. juncea ( Figure 2) yet found to be statistically insignificant. However, when the plants were exposed to 300 mM NaCl, both species showed contrasting results. For example, salt stress resulted in significantly marked decreases in the levels of these pigments in B. napus. In B. juncea, the levels of these pigments did not fall below the control level but rather increased after the salt treatment. For instance, the chlorophyll contents were decreased to almost half in the B. napus Brassica napus (grey bars) were exposed to either 0 or 300 mM NaCl. Different growth parameters, such as plant height (a), leaf area (b), shoot dry weight (c), and root dry weight (d), were determined after 15 days of the treatment. Data points represent the means ± SD of three biological replicates. Significant differences are shown with different asterisks (***, **, * at p = 0.001, 0.01, 0.05, respectively).

Effects of Salinity on Pigments and Ion Concentration
Plants of B. napus and B. juncea were grown in pots and exposed to 300 mM NaCl stress. The concentrations of photosynthetic pigments such as chlorophyll and carotenoids and the chlorophyll a to chlorophyll b ratio were determined. Under control conditions, all these parameters were higher in B. napus than in B. juncea ( Figure 2) yet found to be statistically insignificant. However, when the plants were exposed to 300 mM NaCl, both species showed contrasting results. For example, salt stress resulted in significantly marked decreases in the levels of these pigments in B. napus. In B. juncea, the levels of these pigments did not fall below the control level but rather increased after the salt treatment. For instance, the chlorophyll contents were decreased to almost half in the B. napus plants  (Figure 2a). In addition to light harvesting, carotenoids play many important roles in plants, including photoprotection and the biosynthesis of antioxidants. High NaCl treatment resulted in a considerable reduction in carotenoid contents in B. napus (−87%), indicating less photoprotection of the PSII reaction centers from oxidative stress. In contrast, the concentration of carotenoids under NaCl significantly increased in B. juncea (+67%; Figure 2b). The chlorophyll a/b ratio-a general indicator of the extent of stress that are plants subjected to-was slightly increased in B. juncea (+12%) after exposing the plants to NaCl stress, while a significant drop (−61%) was observed in the B. napus plants, an indication of severe oxidative stress.

EER REVIEW
4 of 17 plants while they doubled in B. juncea under NaCl stress (Figure 2a). In addition to light harvesting, carotenoids play many important roles in plants, including photoprotection and the biosynthesis of antioxidants. High NaCl treatment resulted in a considerable reduction in carotenoid contents in B. napus (−87%), indicating less photoprotection of the PSII reaction centers from oxidative stress. In contrast, the concentration of carotenoids under NaCl significantly increased in B. juncea (+67%; Figure 2b). The chlorophyll a/b ratio-a general indicator of the extent of stress that are plants subjected to-was slightly increased in B. juncea (+12%) after exposing the plants to NaCl stress, while a significant drop (−61%) was observed in the B. napus plants, an indication of severe oxidative stress.  ) and Brassica napus (grey bars) were exposed to either 0 or 300 mM NaCl and concentrations of different photosynthetic pigments, including chlorophyll (a) and carotenoids (b), and the ratio of chlorophyll a/b (c) was measured after 15 days. Data points represent the means ± SD of three biological replicates. Significant differences are shown with different asterisks (***, ** at p = 0.001, 0.01, respectively).
The concentration of different ions (Na + , K + , and Cl − ) was determined in the leaves of the plants of both species after NaCl treatment ( Figure 3). The level of Na + in the control leaf tissues was similar to that in both B. napus and B. juncea, but when the plants were treated with NaCl, the concentration of Na + was significantly increased in both species but to different extents. For example, a 2-fold increase in the Na + level was observed in B. juncea, whereas a 3-fold increase was noted in the B. napus plants. Both the B. juncea and B. napus plants accumulated almost equal concentrations of Cl − ions under control conditions. A slight increase was recorded in the uptake of Cl − in B. juncea leaves when exposed to NaCl stress. However, the concentration of chloride ions in the leaf tissue was sharply (+211% of control) increased in the B. napus plants after salt treatment. Both species also varied in their retention/depletion of K + in their leaves after salt treatment. Under control conditions, the level of K + was comparable in both B. juncea and B. napus. Salt treatment resulted in a considerably significant decline in the K + level in the leaves of B. napus (−66%) as compared to that of the B. juncea plant leaves (−32%).  ) and Brassica napus (grey bars) were exposed to either 0 or 300 mM NaCl and concentrations of different photosynthetic pigments, including chlorophyll (a) and carotenoids (b), and the ratio of chlorophyll a/b (c) was measured after 15 days. Data points represent the means ± SD of three biological replicates. Significant differences are shown with different asterisks (***, ** at p = 0.001, 0.01, respectively).
The concentration of different ions (Na + , K + , and Cl − ) was determined in the leaves of the plants of both species after NaCl treatment ( Figure 3). The level of Na + in the control leaf tissues was similar to that in both B. napus and B. juncea, but when the plants were treated with NaCl, the concentration of Na + was significantly increased in both species but to different extents. For example, a 2-fold increase in the Na + level was observed in B. juncea, whereas a 3-fold increase was noted in the B. napus plants. Both the B. juncea and B. napus plants accumulated almost equal concentrations of Cl − ions under control conditions. A slight increase was recorded in the uptake of Cl − in B. juncea leaves when exposed to NaCl stress. However, the concentration of chloride ions in the leaf tissue was sharply (+211% of control) increased in the B. napus plants after salt treatment. Both species also varied in their retention/depletion of K + in their leaves after salt treatment. Under control conditions, the level of K + was comparable in both B. juncea and B. napus. Salt treatment resulted in a considerably significant decline in the K + level in the leaves of B. napus (−66%) as compared to that of the B. juncea plant leaves (−32%).  week-old plants of Brassica juncea (black bars) and Brassica napus (grey bars) were exposed to eithe 0 or 300 mM NaCl and concentrations of different ions, Na + (a), Cl − (b), and K + (c), were measured after 15 days, as described in the methods. Data points represent the means ± SD of three biologica replicates. Statistically significant differences are shown with different asterisks (***, **, * at p = 0.001 0.01, 0.05, respectively).

Effect of Salinity on PSII Photochemistry
Chlorophyll fluorescence analysis is a well-established method used to estimate func tional changes in photosynthesis [24]. It provides detailed information about PSII effi ciency in intact leaves under different environmental conditions [25]. Chlorophyll fluo rescence was probed to evaluate the efficiency and fluorescence quenching of PSII, includ ing both the photochemical and non-photochemical forms, in B. napus and B. juncea with and without salt stress ( Figure 4). The maximum quantum efficiency of PSII photochem istry, Fv/Fm, was comparable in the two species under non-stress conditions. However exposure to 300 mM NaCl significantly reduced PSII quantum efficiency in B. napu (−66%), indicating the salt-induced photoinhibition of PSII due to stress. In contrast, the salt treatment did not significantly affect the Fv/Fm levels in B. juncea (Figure 4a). Due to photoinhibition, a reduction in the rate of electron transportation (ETR) was observed in both Brassicas. However, B. napus showed a much higher reduction in this paramete (−70% of control) than B. juncea, which showed only a mild reduction in ETR, confirming the stress-induced photoinhibition of PSII in B. napus (Figure 4b). Likewise, the effective quantum yield of PSII, as denoted by φPSII, was higher in B. juncea under control condi tions. Exposure to NaCl stress reduced the PSII quantum yields in both species; however high NaCl exposure caused substantial PSII quantum yield losses in B. napus (−75%). In contrast, the PSII quantum yield in B. juncea did not fall below the control level, even afte 300 mM NaCl stress; rather, an increase in this parameter was observed after the salt stress ( Figure 4c).
When plants undergo stress, they protect themselves from stress by increasing the dissipation of absorbed energy as heat, termed as the non-photochemical quenching o chlorophyll fluorescence, measured as regulated energy dissipation (NPQ) and nor-regu lated energy dissipation (Y(NO)) [26]. The NPQ was almost equal in the unstressed plants of both species. However, it was considerably increased in B. napus, consistent with the earlier observations of PSII photoinhibition (Figure 5a). In contrast, B. juncea did not show any increase in NPQ, but it decreased after salt stress, in line with the earlier observations of the higher photoprotection of PSII in B. juncea. In the unstressed plants, Y(NO) was higher in B. juncea than in B. napus and showed no significant increase after salt treatment The B. napus plants showed a sharp increase in non-regulated heat dissipation (+121% o control; p ≤ 0.01%) when exposed to a 300 mM salt concentration (Figure 5b). Brassica napus (grey bars) were exposed to either 0 or 300 mM NaCl and concentrations of different ions, Na + (a), Cl − (b), and K + (c), were measured after 15 days, as described in the methods. Data points represent the means ± SD of three biological replicates. Statistically significant differences are shown with different asterisks (***, **, * at p = 0.001, 0.01, 0.05, respectively).

Effect of Salinity on PSII Photochemistry
Chlorophyll fluorescence analysis is a well-established method used to estimate functional changes in photosynthesis [24]. It provides detailed information about PSII efficiency in intact leaves under different environmental conditions [25]. Chlorophyll fluorescence was probed to evaluate the efficiency and fluorescence quenching of PSII, including both the photochemical and non-photochemical forms, in B. napus and B. juncea with and without salt stress ( Figure 4). The maximum quantum efficiency of PSII photochemistry, F v /F m , was comparable in the two species under non-stress conditions. However, exposure to 300 mM NaCl significantly reduced PSII quantum efficiency in B. napus (−66%), indicating the salt-induced photoinhibition of PSII due to stress. In contrast, the salt treatment did not significantly affect the F v /F m levels in B. juncea ( Figure 4a). Due to photoinhibition, a reduction in the rate of electron transportation (ETR) was observed in both Brassicas. However, B. napus showed a much higher reduction in this parameter (−70% of control) than B. juncea, which showed only a mild reduction in ETR, confirming the stress-induced photoinhibition of PSII in B. napus ( Figure 4b). Likewise, the effective quantum yield of PSII, as denoted by ϕPSII, was higher in B. juncea under control conditions. Exposure to NaCl stress reduced the PSII quantum yields in both species; however, high NaCl exposure caused substantial PSII quantum yield losses in B. napus (−75%). In contrast, the PSII quantum yield in B. juncea did not fall below the control level, even after 300 mM NaCl stress; rather, an increase in this parameter was observed after the salt stress ( Figure 4c).
When plants undergo stress, they protect themselves from stress by increasing the dissipation of absorbed energy as heat, termed as the non-photochemical quenching of chlorophyll fluorescence, measured as regulated energy dissipation (NPQ) and nor-regulated energy dissipation (Y(NO)) [26]. The NPQ was almost equal in the unstressed plants of both species. However, it was considerably increased in B. napus, consistent with the earlier observations of PSII photoinhibition (Figure 5a). In contrast, B. juncea did not show any increase in NPQ, but it decreased after salt stress, in line with the earlier observations of the higher photoprotection of PSII in B. juncea. In the unstressed plants, Y(NO) was higher in B. juncea than in B. napus and showed no significant increase after salt treatment. The B. napus plants showed a sharp increase in non-regulated heat dissipation (+121% of control; p ≤ 0.01%) when exposed to a 300 mM salt concentration (Figure 5b).

black bars) and
Brassica napus (grey bars) were exposed to either 0 or 300 mM NaCl and different photosynthesis parameters such as the maximum quantum efficiency of PSII, Fv/Fm (a), electron transport rate, ETR (b) and the quantum yield of PSII, φPSII (c) were measured. Data points represent the means ± SD of three biological replicates. Significant differences are shown with different asterisks (***, **, * at p = 0.001, 0.01, 0.05, respectively).

Figure 5.
Effect of NaCl stress on non-photochemical PSII chlorophyll fluorescence in Brassica. Plants of Brassica juncea (black bars) and Brassica napus (grey bars) were exposed to either 0 or 300 mM NaCl and non-photochemical quenching of chlorophyll fluorescence was determined by recording regulated heat dissipation, NPQ (a), and nor-regulated energy dissipation, (Y(NO) (b). Data points represent the means ± SD of three biological replicates. Significant differences are shown with different asterisks (***, ** at p = 0.001 and 0.01, respectively).

Effect of Salt Stress on Light Response Curves
Plants adapt themselves to environmental changes to sustain their growth and yield. These adaptation strategies may include changes in metabolism and photosynthesis [27,28]. Light response curves (LRCs) describe the impact of light variations on photosynthesis by providing information about the maximum photosynthetic capacity and yield [29]. Therefore, LRCs show how well plants acclimate their photosynthetic performance in variable environments when subjected to changes like salt stress [30].
The LRCs showed significant differences between the control and salt-stressed plants of B. juncea and B. napus ( Figure 6). When the light intensity was increased, the ETR was also increased in both genotypes. However, this increase in the ETR was much higher in B. juncea than in B. napus. Contrary to the ETR, a decreasing trend was observed in both Brassica napus (grey bars) were exposed to either 0 or 300 mM NaCl and different photosynthesis parameters such as the maximum quantum efficiency of PSII, F v /F m (a), electron transport rate, ETR (b) and the quantum yield of PSII, ϕPSII (c) were measured. Data points represent the means ± SD of three biological replicates. Significant differences are shown with different asterisks (***, **, * at p = 0.001, 0.01, 0.05, respectively).

black bars) and
Brassica napus (grey bars) were exposed to either 0 or 300 mM NaCl and different photosynthesis parameters such as the maximum quantum efficiency of PSII, Fv/Fm (a), electron transport rate, ETR (b) and the quantum yield of PSII, φPSII (c) were measured. Data points represent the means ± SD of three biological replicates. Significant differences are shown with different asterisks (***, **, * at p = 0.001, 0.01, 0.05, respectively).

Figure 5.
Effect of NaCl stress on non-photochemical PSII chlorophyll fluorescence in Brassica. Plants of Brassica juncea (black bars) and Brassica napus (grey bars) were exposed to either 0 or 300 mM NaCl and non-photochemical quenching of chlorophyll fluorescence was determined by recording regulated heat dissipation, NPQ (a), and nor-regulated energy dissipation, (Y(NO) (b). Data points represent the means ± SD of three biological replicates. Significant differences are shown with different asterisks (***, ** at p = 0.001 and 0.01, respectively).

Effect of Salt Stress on Light Response Curves
Plants adapt themselves to environmental changes to sustain their growth and yield. These adaptation strategies may include changes in metabolism and photosynthesis [27,28]. Light response curves (LRCs) describe the impact of light variations on photosynthesis by providing information about the maximum photosynthetic capacity and yield [29]. Therefore, LRCs show how well plants acclimate their photosynthetic performance in variable environments when subjected to changes like salt stress [30].
The LRCs showed significant differences between the control and salt-stressed plants of B. juncea and B. napus ( Figure 6). When the light intensity was increased, the ETR was also increased in both genotypes. However, this increase in the ETR was much higher in B. juncea than in B. napus. Contrary to the ETR, a decreasing trend was observed in both Effect of NaCl stress on non-photochemical PSII chlorophyll fluorescence in Brassica.
Plants of Brassica juncea (black bars) and Brassica napus (grey bars) were exposed to either 0 or 300 mM NaCl and non-photochemical quenching of chlorophyll fluorescence was determined by recording regulated heat dissipation, NPQ (a), and norregulated energy dissipation, (Y(NO) (b). Data points represent the means ± SD of three biological replicates. Significant differences are shown with different asterisks (***, ** at p = 0.001 and 0.01, respectively).

Effect of Salt Stress on Light Response Curves
Plants adapt themselves to environmental changes to sustain their growth and yield. These adaptation strategies may include changes in metabolism and photosynthesis [27,28]. Light response curves (LRCs) describe the impact of light variations on photosynthesis by providing information about the maximum photosynthetic capacity and yield [29]. Therefore, LRCs show how well plants acclimate their photosynthetic performance in variable environments when subjected to changes like salt stress [30].
The LRCs showed significant differences between the control and salt-stressed plants of B. juncea and B. napus (Figure 6). When the light intensity was increased, the ETR was also increased in both genotypes. However, this increase in the ETR was much higher in B. juncea than in B. napus. Contrary to the ETR, a decreasing trend was observed in both species for an effective quantum yield of PSII, ϕPSII. The means of Y(II) at the 0 mM NaCl concentration were similar in the two genotypes, whereas the mean was~3-fold lower in the B. napus plants at 300 mM NaCl compared to B. juncea. genome (NHX1 and AVP1) as well as chloroplast genome (psbA, psaA, petB, rbcL) involved in salt tolerance and photosynthesis. NHX1 and AVP1 are nuclear-encoded enzymes and responsible for the transportation of Na + /K + and H + , respectively. NHX1 encodes for the vacuolar Na + /H + antiporter involved in ionic homeostasis, salt tolerance, and leaf development [31], while AVP1 encodes for a vacuolar H + -pyrophosphatase, which enables plants to withstand drought and salt stress regimes [32]. The relative quantitative expression of the nuclear-encoding NHX1 and AVP1 genes was increased in both B. juncea and B. napus under high salt stress compared to the control. However, the transcript level of these genes was much higher in B. juncea than in B. napus. For example, the expression of NHX1 was increased ~3-fold in B. juncea under stress while it was only increased 1.3-fold in B. napus (Figure 7). The AVP1 gene showed a slight increase in B. napus, while a ~100% increase was observed in the B. juncea plants. Figure 6. Electron transport rate and effective PSII efficiency in Brassica plants. Brassica juncea (black bars) and Brassica napus (grey bars) plants exposed to either 0 or 300 mM NaCl were used for the determination of the electron transport rate, ETR (a) and effective PSII efficiency, φPSII (b), in response to different light intensities at a CO2 partial pressure of 400 ppm. Data points represent the means ± SD of three biological replicates. Significant differences are shown with different asterisks (***, * at p = 0.001 and 0.05, respectively).
As it was observed that the PSII in B. juncea was found to be less affected by NaCl stress compared to that in B. napus, we were interested in determining the effects of salt stress on different photosynthetic complexes, such as PSI, PSII, the cytochrome b6f complex and RuBisCo (Ribulose-1,5-bisphosphate carboxylase/oxygenase), the key enzyme of the Kelvin cycle. For this, we studied the expression of chloroplast-encoded genes-psbA, psaA, petB, and rbcL-coding for the proteins which make the core components of these complexes (Figure 8). When the plants were exposed to salt stress, the expression of the psbA gene, which encodes D1, is the core component of the PSII reaction center, and is highly sensitive to stress [33], was increased in both species. However, this increase was twice as high in B. juncea than in B. napus (Figure 8a). Likewise, the expression of the PSI gene, psaA, was doubled in the B. juncea plants under salt stress while decreasing in B. napus (Figure 8b). The expression of the petB gene, which encodes a component of the Cyt b6f complex, was downregulated in B. juncea under NaCl stress while showing a marked Figure 6. Electron transport rate and effective PSII efficiency in Brassica plants. Brassica juncea (black bars) and Brassica napus (grey bars) plants exposed to either 0 or 300 mM NaCl were used for the determination of the electron transport rate, ETR (a) and effective PSII efficiency, ϕPSII (b), in response to different light intensities at a CO 2 partial pressure of 400 ppm. Data points represent the means ± SD of three biological replicates. Significant differences are shown with different asterisks (***, **, * p = 0.001, 0.01, 0.05, respectively).

Effect of Salinity on the Relative Gene Expression of Different Genes
Since B. juncea and B. napus showed variable growth and photosynthetic performance when exposed to 300 mM NaCl, B. juncea appeared to be more salt-tolerant than B. napus in terms of growth and photosynthesis under stress. To explain the genetic differences in these two species, we examined the expression of different genes located in the nuclear genome (NHX1 and AVP1) as well as chloroplast genome (psbA, psaA, petB, rbcL) involved in salt tolerance and photosynthesis. NHX1 and AVP1 are nuclear-encoded enzymes and responsible for the transportation of Na + /K + and H + , respectively. NHX1 encodes for the vacuolar Na + /H + antiporter involved in ionic homeostasis, salt tolerance, and leaf development [31], while AVP1 encodes for a vacuolar H + -pyrophosphatase, which enables plants to withstand drought and salt stress regimes [32]. The relative quantitative expression of the nuclear-encoding NHX1 and AVP1 genes was increased in both B. juncea and B. napus under high salt stress compared to the control. However, the transcript level of these genes was much higher in B. juncea than in B. napus. For example, the expression of NHX1 was increased~3-fold in B. juncea under stress while it was only increased 1.3-fold in B. napus (Figure 7). The AVP1 gene showed a slight increase in B. napus, while a~100% increase was observed in the B. juncea plants.
As it was observed that the PSII in B. juncea was found to be less affected by NaCl stress compared to that in B. napus, we were interested in determining the effects of salt stress on different photosynthetic complexes, such as PSI, PSII, the cytochrome b 6 f complex and RuBisCo (Ribulose-1,5-bisphosphate carboxylase/oxygenase), the key enzyme of the Kelvin cycle. For this, we studied the expression of chloroplast-encoded genes-psbA, psaA, petB, and rbcL-coding for the proteins which make the core components of these complexes (Figure 8). When the plants were exposed to salt stress, the expression of the psbA gene, which encodes D1, is the core component of the PSII reaction center, and is highly sensitive to stress [33], was increased in both species. However, this increase was twice as high in B. juncea than in B. napus (Figure 8a)   increase in B. napus (Figure 8c). A 4-fold increase in the expression of th enzyme, RuBisCo, was observed in B. juncea under stress, while a slight inc orded in B. napus (Figure 8d).

Discussion
Brassica is an important source of edible oil and, like other crops, experiences yield reduction due to abiotic stresses, including salinity. Most Brassica crops are predominantly cultivated in arid and semi-arid areas and often experience saline stress [34]. In this study, we assessed the salt tolerance potential of two commonly cultivated Brassica species with distinct genomes, B. juncea and B. napus. Our data show that B. juncea is more salt-tolerant than B. napus due to its regulated ion transport and better PSII-protective mechanisms. A high degree of salt tolerance and better regeneration potential of Brassica juncea [21] make this genotype a suitable candidate for genetic manipulations aimed towards the development of climate-smart, stress-tolerant oilseed crops.
The exposure of plants to 300 mM NaCl significantly reduced the growth of both species. However, this growth was more depressed in B. napus than in B. juncea (Figure 1). Similar results for B. napus were reported in a study by Wu et al. [35], in which a decrease in the shoot and root lengths of B. napus at 200 mM NaCl was observed. Similar findings have been reported [36][37][38]. Rezaei et al. [39] stated that salinity stress also decreased the average leaf area in Brassica, a result which is similar to our findings. We observed a decrease in average leaf size in both species, but this reduction was much higher in the B. napus plants. The higher salt tolerance observed in B. juncea has long been recognized [40]. This differential salt tolerance among Brassicas seems to be controlled by QTLs [41].
A plant's salt sensitivity or resilience reflects its internal ionic balance [42]. In Brassicas, salt tolerance correlates with the transcription of the Na + /H + antiporter (NHX1) that transports Na + into vacuoles from the cytosol [23,43]. Na + and Cl − , particularly when accumulated in photosynthesizing tissues, have been found to be toxic to plants [44][45][46]. The accumulation of higher levels of Na + and Cl − in B. napus with a lower expression of NHX1 and H-PPase (AVP1) as compared to B. juncea is indicative of ionic toxicity in the cytoplasm due to the insufficient vacuolar compartmentalization of toxic ions.
The observed effect of salinity stress on photosynthetic pigments in B. napus, namely, depletion, could be a combination of the inhibition of chlorophyll biosynthesis and/or increased degradation under salinity. A reduction in chlorophyll pigments is a sign of oxidative stress [47]. It represents the photoprotective mechanism of salt-sensitive plants, through which they decrease their light absorbance via the depletion of their chlorophyll contents [48]. The maintenance of photosynthetic pigments in B. juncea reflects its high tolerance to NaCl stress, an observation in line with earlier studies [49,50]. As reported earlier, the elevated levels of chlorophyll could be due to the more significant number of chloroplasts in stressed leaves [51]. Carotenoids are pigments that act as antioxidants and protect membrane lipids from oxidative stress [5]. We observed an increase in the carotenoid contents in B. juncea and a decrease in B. napus, with carotenoids having a significant role in the photoprotective mechanism. Studies have reported increased carotenoid contents under salt stress in salt-tolerant cultivars of B. juncea [52] and other Brassica species [53].
In this work, chlorophyll fluorescence was employed to investigate the impact of salt stress on PSII efficiency in Brassica plants. Various PSII parameters that provide information on the performance of the photosynthetic apparatus were studied [54]. An increase in non-photochemical quenching parameters and a decrease in overall PSII performance and photochemical quenching parameters reflect a generalized response of plants to salt stress [55]. This generalized response was also documented in this work. For example, F v /F m , which we considered as the primary salt stress indicator [56,57], remained unaffected in B. juncea under stress but significantly decreased in B. napus. These findings are in line with results reported in different studies, where no change was found in this parameter after salt stress in Thellungiella halophila [58], salt-tolerant barley [59], and rice plants [60]. Moreover, lowered values of F v /F m indicate the inactivation of PSII reaction centers due to stress [61][62][63]. As observed in this study, a decrease in F v /F m in different Brassica species was also reported by Pavlovic et al. (2019) [23]. This implied that the B. napus PSII experienced more damage than that of B. juncea. This line of evidence is further supported by the increases in non-photochemical quenching parameters in B. napus under stress (Figure 4). These observations are in line with various studies that reported similar trends in chlorophyll fluorescence quenching parameters in different species [55,64,65]. The drop in ETR in B. napus is in line with earlier results, with different studies reporting a salt-stress-induced decrease in ETR and PSII efficiency [38,58,66]. Overall, the maintenance of higher F v /F m , ETR, and PSII efficiency in B. juncea highlights its ability to sustain a decent photosynthetic performance, even under high salt stress. Furthermore, the increases in PAR did not increase the ETR or PSII efficiency in B. napus ( Figure 6). The light response curves (LRCs) were used to elucidate the response of photosynthesis to varying light intensities, dependent upon the nature of the species and environmental conditions, such as abiotic stress [67]. Similar results were observed in two different plant species of the family Hymenophyllaceae in a study that reported a decrease in PSII efficiency and an increase in NPQ with increasing intensities of light, a finding which is consistent with our results [68].
The differential expression of different genes points towards the different genetics in these two species. Studies have shown that the overexpression of both NHX1 [16,17,69] and AVP1 [69][70][71] confers tolerance against salt stress, including other abiotic stresses. The psaA and psbA genes encode for the chlorophyll apoproteins of PSI (A1) and PSII (D1), respectively. High production of A1 and D1 proteins in B. juncea might have contributed to better photosynthetic performance and salt tolerance in this species compared to B. napus. It was recently shown that the overexpression of D1 in Arabidopsis, rice, and tobacco made transgenic plants stress-tolerant, producing high yields under stress conditions [33,72]. An increased expression of rbcL indicates better CO 2 fixation and good photosynthetic activity in B. juncea plants, even under high salinity [73]. These results are supported by the findings of Li et al. (2021), where rbcL expression was more significant under high light and salinity [74]. Likewise, the reduction in PetB in B. juncea was also in line with the findings of Wang et al., who reported that the expression of photosynthetic genes related to electron transport, such as PetA, PetB, and PetE, was significantly reduced under saline stress [75].
The performance of B. juncea in terms of growth, as well as the maintenance of photosynthetic pigments and ionic homeostasis, was far better than that of B. napus at the 300 mM NaCl concentration. Similarly, the high levels of F v /F m , PSII efficiency, and ETR, as well as the reduction in energy loss, as measured using NPQ and Y(NO), indicate that B. juncea is highly salt-tolerant, which makes it a good candidate for cultivation on marginal lands. Furthermore, the differential expression of genes under salt stress in B. juncea and B. napus implies genetic differences between these two species. The high salt tolerance of the commercial cultivar of B. juncea cv. Aari canola that was used in this study and its better regeneration potential, as reported earlier [21], would allow for the direct transformation of commercial cultivars other than Westar, which is used as a model for Brassica. The direct transformation of commercial cultivars offers several advantages over the traditional approaches for engineering a trait into the model and then transferring this trait into commercial cultivars through crossing and backcrossing.

Plant Growth Conditions
Seeds of B. juncea and B. napus were obtained from the Oilseed Research Institute, Faisalabad, and grown under controlled temperature, light, and humidity (relative humidity: 32 ± 5%; temperature: 25/18 • C day/night; 12 h day/light; light intensity: 500 ± 50 µmol photons m −2 s −1 ). The seeds were germinated in plastic pots (25 cm height and 19 cm diameter) filled with loamy soil and irrigated with 0.5X Hoagland solution.

Salinity Treatment, Experimental Design, and Growth Parameters
In total, 24 plants of each species were divided into two (02) groups. The plants were sub-irrigated with 2 L of 0.5X Hoagland solution of pH 6.5-7.0 with or without 300 mM NaCl in 12 cm × 30 cm trays [76]. The salinity treatment was started when the plants were around 6 cm in height with five (05) leaves, using 50 mM NaCl day −1 increments to avoid osmotic shock. The salt concentrations were measured daily with a refractometer (ATAGO S-10E, Tokyo, Japan). The water loss was adjusted by replenishing it to the required level with distilled water.
The plants were harvested after exposure to the given concentration of NaCl after two (02) weeks. We measured different plant growth parameters such as fresh weight and plant and root lengths. The fresh weight was recorded immediately after harvest. The plant samples were dried in an oven at 80 • C for 48 h for the dry weight measurement. The following equation was used to calculate the water content (WC) in the leaf and root tissues: The leaf area was determined using ImageJ [77]. The experiments were carried out in three replicates, and all measurements were carried out in three biological replicates.

Determination of Ion (Na + , K + , Cl − ) Accumulation
The concentration of different ions (Na + , K + , Cl − ) in the leaves was determined using the hot water extraction method [78]. Briefly, leaf samples were dried and powdered, mixing a fraction (0.1 g) with 10 mL water followed by homogenization at 80 • C for 4 h. The resulting mixture was filtered, followed by dilution with distilled water for the measurement of the ion concentration. The concentration of cations (Na + and K + ) was measured using a flame photometer (Intech model I-66, Wallonia, Belgium), while the measurement of Cl − ions was carried out using an ion-selective electrode and BANTE instrument.

Leaf Pigments
The chlorophyll content in the fourth and fifth leaves of the plant was estimated using SPAD 502 (Konica Minolta, Osaka, Japan). The concentration of different photosynthetic leaf pigments such as chlorophyll a (Chl a ), chlorophyll b (Chl b ), total chlorophyll (Chl t ), and carotenoids (C x+c ) was determined by placing leaf discs of equal size and weight into 4 mL ethanol under dark conditions. The absorbance was read at 470, 644, and 662 nm [79]. The concentrations were calculated using the following formulae:

Chlorophyll Fluorescence Measurements
Chlorophyll fluorescence measurements were conducted using a PAM 2500 instrument (Walz). The plants were dark-adapted for 20-30 min to allow for the dissipation of electrons from the photosystems. Minimal fluorescence (F o ) and maximal fluorescence (F m ) were measured using actinic light (<0.1 µmol photons m −2 s −1 ) and a saturating pulse of 10,000 µmol photons m −2 s −1 for 0.6 s, respectively [59]. The maximum quantum efficiency of PSII (F v /F m ) was calculated using The steady-state (F s ) and maximal fluorescence (F m ) were measured in light-adapted plants. Different parameters such as the effective photochemical quantum yield of PSII (Y(II)), photochemical fluorescence quenching (qP), non-photochemical fluorescence quenching (NPQ), the quantum yield of regulated heat dissipation (Y(NPQ), the quantum yield of non-regulated heat dissipation (Y(NO)), and the relative electron transport rate (rETR) were calculated using the following formulae [80][81][82][83]: The values of Y(NPQ) and Y(NO) were calculated at a PPFD ≈ 700 µmol photons m −2 s −1 [84]: rETR = ϕPSII × PPFD × 0.5 × 0.84 (1) where ϕPSII is the effective quantum yield of PSII, PPFD is the photosynthetic photon flux density incident on the leaf surface, 0.5 represents the fraction of light absorbed by the PSII assuming an equal light energy distribution between PSII and PSI, and 0.84 represents the average proportion of light absorbed by the leaf [80].

RNA Extraction and qRT-PCR Analysis
Total RNA was extracted from the leaves of the treated (300 mM) and non-treated (0 mM) B. juncea and B. napus plants using the Tri-reagent method. For this purpose, approximately 100 mg of fresh leaf sample was ground in 1 mL of Tri-reagent using a pre-chilled mortar and pestle and kept on ice until further processing. The samples were incubated at room temperature for 5 min and then centrifuged at 13,000 rpm for 10 min at 4 • C. The supernatant was collected in a fresh microfuge tube, and 200 µL chloroform was added to it, followed by vigorous shaking for 15 s, and it was then kept at room temperature for 5-10 min. This was followed by centrifugation again at 13,000 rpm for 15 min at 4 • C. The clear aqueous phase was carefully transferred into a new tube, and 250 µL chilled isopropanol and 250 µL chilled 0.8 M sodium citrate solutions were added to the tubes, followed by inversion 6-8 times for gentle mixing. The samples were then incubated at room temperature for 5-10 min and centrifuged at 13,000 rpm for 10 min at 4 • C. The RNA pellet was obtained and washed with 1 mL of 75% ethanol. The tubes were centrifuged at 10,000 rpm for 5 min at 4 • C. The ethanol was removed, and the pellets were kept for air drying for 5 min at room temperature. The RNA was dissolved in 50 µL of autoclaved nuclease-free water and stored at −80 • C for further use. The isolated RNA was treated with DNase-1 to remove any potential DNA contaminations, and sample purity was estimated by calculating the A260/280 ratios followed by visualization on an agarose gel. Approximately 5 µg of total RNA was used to synthesize the first strand of cDNA using the RevertAid™ Reverse Transcriptase kit (Fermentas, Heidelberg, Germany), following the manufacturer's protocol. The synthesized cDNA was diluted for further use in qRT-PCR analysis.
The primers for different nuclear and chloroplast genes were synthesized commercially ( Table 1). The qRT-PCR reaction was carried out in a Bio-Rad CFX96TM Touch Real-Time PCR detection system (Bio-Rad, Hercules, CA, USA) in a 15 µL reaction volume comprising 7.5 µL of BrightGreen 2X qPCR MasterMix (ABM, Vancouver, BC, Canada), 0.4 µL of each of forward and reverse primer, 2 µL of diluted cDNA, and 4.7 µL of sterile nuclease-free distilled H 2 O. The PCR conditions for all the genes were optimized as follows: initial denaturation at 95 • C for 5 min followed by 35 cycles of 95 • C for 30s, 55 • C for 30s, and 72 • C for 30s. Melt curve analysis was executed using constant heating from 55 • C to 95 • C followed by 95 • C for 5s. All reactions were carried out in two technical replicates. The Actin-β (Act-β) gene was used as an internal control. The relative expression of each gene was calculated using the 2 −∆∆Ct method or Livak method [85], which directly uses the threshold cycles (Ct) to measure the fold change in gene expression, as given below:

Statistical Analyses
All statistical analyses were carried out in an R environment 4.1.3 for Windows. The data on the obtained traits were analyzed for significance through either one-way or two-way ANOVA to analyze differences between groups and variables. The R package, Agricolae, was used to carry out a pairwise comparison of means using Tukey's honest significant difference (HSD) post hoc test.

Conclusions
In conclusion, this study illustrated that Brassica juncea exhibits greater salt tolerance than Brassica napus. Our data provide strong evidence for this, citing regulated ion transport, effective photosystem II (PSII) protective mechanisms, and robust genetic attributes as pivotal factors. Notably, B. juncea demonstrated superior capabilities in maintaining ionic homeostasis, retaining photosynthetic pigments, and expressing genes vital for salt stress resilience compared to B. napus when exposed to 300 mM NaCl stress. We have found that this higher tolerance can be attributed to the regulated compartmentalization of toxic ions through the enhanced expression of the NHX1 and AVP1 genes in B. juncea. Our research also highlighted the capacity of B. juncea to maintain an efficient photosynthetic performance under high salt stress, together with its ability to sustain higher F v /F m , ETR, and PSII efficiency levels. Furthermore, B. juncea demonstrated higher resistance to oxidative stress and a more effective photoprotective mechanism, evidenced by the preservation of photosynthetic pigments and the increase in carotenoid content, which significantly shielded the plants from oxidative damage.
Our research highlights the potential of B. juncea as a climate-resilient crop that is suitable for cultivation in arid and semi-arid regions subject to high-salinity conditions. Further studies are recommended to explore the detailed genetic mechanisms behind these differences in salt tolerance between B. juncea and B. napus.