Expression of EPL1 from Trichoderma atroviride in Arabidopsis Confers Resistance to Bacterial and Fungal Pathogens

During plant interaction with beneficial microorganisms, fungi secrete a battery of elicitors that trigger plant defenses against pathogenic microorganisms. Among the elicitor molecules secreted by Trichoderma are cerato-platanin proteins, such as EPL1, from Trichoderma atroviride. In this study, Arabidopsis thaliana plants that express the TaEPL1 gene were challenged with phytopathogens to evaluate whether expression of EPL1 confers increased resistance to the bacterial pathogen Pseudomonas syringae and the necrotrophic fungus Botrytis cinerea. Infection assays showed that Arabidopsis EPL1-2, EPL1-3, EPL1-4 expressing lines were more resistant to both pathogens in comparison to WT plants. After Pseudomonas syringae infection, there were reduced disease symptoms (e.g., small chlorotic spots) and low bacterial titers in the three 35S::TaEPL1 expression lines. Similarly; 35S::TaEPL1 expression lines were more resistant to Botrytis cinerea infection, showing smaller lesion size in comparison to WT. Interestingly, an increase in ROS levels was detected in 35S::TaEPL1 expression lines when compared to WT. A higher expression of SA- and JA-response genes occurred in the 35S::TaEPL1 lines, which could explain the resistance of these EPL1 expression lines to both pathogens. We propose that EPL1 is an excellent elicitor, which can be used to generate crops with improved resistance to broad-spectrum diseases.


Introduction
The genus Trichoderma comprises many rhizocompetent filamentous fungi found in different ecosystems [1]. Trichoderma species are fast-growing, opportunistic invaders and prolific producers of secondary metabolites with antibiotic activity that suppresses diverse phytopathogens such as bacteria, fungi, and nematodes [2][3][4]. These properties make these fungi an ecologically dominant species. Other biocontrol mechanisms used by Trichoderma include mycoparasitism and competition for space and nutrients [5]. Due to the ability of Trichoderma spp. to suppress different phytopathogenic fungi, some species have been widely used in agriculture as biological control agents [6,7]. Some Trichoderma species have the ability to establish symbiotic relationships with plants. This symbiosis is achieved through crosstalk, in which plants and Trichoderma produce a wide range of chemical compounds that modify the transcriptomes, proteomes, and metabolomes of host plants [8,9]. As a result, Trichoderma species produce a wide range expressing TaEPL1. We also observed high levels of hydrogen peroxide accumulation in TaEPL1 expressing lines compared to WT plants. Expression analysis of plant hormonerelated genes showed that the transgenic EPL1 lines induced SA and JA related genes, which could explain the enhanced resistance of these transgenic lines to the tested pathogens.

The Expression of the EPL1 Gene in Arabidopsis Results in Accelerated Growth
The expression of the Trichoderma EPL1 gene in Arabidopsis (35S::TaEPL1 lines) resulted in a positive impact on its growth, as observed in 21-day-old plants grown in soil ( Figure 1A). In particular, the 35S::TaEPL1-4 line displayed the highest development among the three TaEPL1 expressing lines ( Figure 1A). As previously observed, 40-day-old plants of the 35S::TaEPL1-4 exhibited the most significant development, while the parental Col-0 (WT) displayed the lowest growth ( Figure 1B). We evaluated the fresh weight of the aerial parts of each 40-day-old plant, and all three TaEpl1 expressing lines exhibited higher fresh weights than the WT. Among them, the 35S::TaEpl1-4 line exhibited the highest biomass, as evidenced by a greater number of developed inflorescences ( Figure 1B,C). . Data are means ± SE (n = 6). Statistical analysis between genotypes was determined by One-way ANOVA, and the letters indicate statistically significant differences by Tukey's test at p < 0.05. . Data are means ± SE (n = 6). Statistical analysis between genotypes was determined by One-way ANOVA, and the letters indicate statistically significant differences by Tukey's test at p < 0.05.
2.3. The Arabidopsis 35S::TaEPL1 Lines Are More Resistant to Infection by Pseudomonas Syringae The Arabidopsis TaEPL1 expression lines were inoculated with P. syringae pv. tomato DC3000 strain (Pst) to examine whether the expression of the TaEPL1 elicitor confers resistance against bacterial infection. Four-week-old Arabidopsis EPL1-2, EPL1-3, EPL1-4, and WT plants were inoculated with Pst bacterial suspension. Disease symptoms and bacteria colony-forming units were recorded after 72 hpi ( Figure 2). We observed more severe symptoms with larger chlorotic spots in the WT plants than the 35S::TaEPL1 expression lines ( Figure 2A). This is in agreement with the low levels of bacterial titers detected in the Arabidopsis 35S::TaEPL1 expression lines, which were up to 14.8-fold lower than that obtained in the WT plants ( Figure 2B). The EPL1-3 line presented the lowest bacterial titers (6.3 × 10 3 ) at 72 hpi and showed milder symptoms of Pst infection in comparison to the other two overexpressing lines ( Figure 2B). This correlates with the EPL1-3 line having the highest EPL1 transcript levels among the transgenic lines (Supplementary Figure S1). Our data reveal that expression of the EPL1 gene from the beneficial fungus Trichoderma in Arabidopsis plants confers marked resistance to P. syringae infection. In plants, ROS play an important role in plant defense against phytopathogen infection [30]. We analyzed whether the 35S::TaEPL1 lines produced a higher amount of ROS as a possible mechanism for resistance against microbes. We detected ROS in root tips of 10day-old 35S::TaEPL1 and WT plantlets using 2,7-dichlorofluorescein diacetate (DCFH2-DA) dye and fluorescence microscopy. ROS signal, detected as green fluorescence, was more intense in 35S::TaEPL1 lines compared to WT roots ( Figure 3A). Catalase (CAT) is a key antioxidant enzyme that alleviates oxidative stress through decomposition of hydrogen peroxides to water and oxygen. The CAT treatment was carried out to determine its effect on H 2 O 2 levels between the 35S::TaEPL1 and WT lines. When we applied 250 U/mL CAT on 35S::TaEPL1 and WT plants, H 2 O 2 -associated fluorescence in the root tips in all transgenic lines was decreased, whereas in WT plants, no signal was detected ( Figure 3A). A quantitative analysis of H 2 O 2 confirmed that 35S::TaEPL1 lines accumulated a higher amount of hydrogen peroxide, with the highest levels observed in the 35S::TaEPL1-3 line (3-fold more than WT) ( Figure 3B). Moreover, upon the application of CAT enzyme (250 U/mL), both the transgenic 35S::TaEPL1 lines and the WT had decreased H 2 O 2 levels, but the transgenic lines maintained the highest values ( Figure 3B).  Representative images in root tips of ten-days-old WT and 35S::TaEPL1 seedlings (TaEPL1-2, TaEPL1-3, and TaEPL1 Figure 4). This same behavior was observed in the mock inoculated plants, in which all the measured markers showed a higher expression in the 35S::TaEPL1-3 line than the WT (Figure 4). This result is in accordance with the observation that this EPL1 expressing line is also the most resistant to P. syringae.  Values were expressed as relative expression levels (2 −ΔCt ) calculated after normalization to the A. thaliana UBQ5 gene. For each sample, three biological replicates were analyzed with their respective technical replicates. The variance analysis was done by Two-way ANOVA (genotype x infection), and the letters indicate statistically significant differences by Tukey's test at p < 0.05.

The Arabidopsis 35S::TaEPL1 Lines Were More Resistant to Botrytis cinerea Infection
The resistance of Arabidopsis EPL1-expressing plants against infection by the necrotrophic fungus B. cinerea strain B05.10 was assessed. Four-week-old plants expressing EPL1 gene (EPL1-2, -3, -4) were inoculated with the B. cinerea spore solution and compared with WT plants. After 72 hpi, the incidence of infection and leaf lesion size were evaluated. Figure 5A shows that the 35S::TaEPL1 expressing lines were more resistant to fungus infection than WT plants, as observed by decreased leaf necrosis and less water-soaking development. Necrotic lesion formation in the 35S::TaEPL1 lines showed reductions in a lesion size of 4.39 ± 0.1 mm 2 for EPL1-3, 5.07 ± 0.1 mm 2 for the EPL1-4 line and 6.13 ± 0.2  Figure 5A shows that the 35S::TaEPL1 expressing lines were more resistant to fungus infection than WT plants, as observed by decreased leaf necrosis and less water-soaking development. Necrotic lesion formation in the 35S::TaEPL1 lines showed reductions in a lesion size of 4.39 ± 0.1 mm 2 for EPL1-3, 5.07 ± 0.1 mm 2 for the EPL1-4 line and 6.13 ± 0.2 mm 2 for the EPL1-2 line, while the WT leaves had lesions of 7.77 ± 0.2 mm 2 ( Figure 5C). Even though disease incidence was not strongly reduced in the EPL1 expressing lines, the EPL1-3 line had the lowest necrotic lesion size caused by the fungus (Figure 5B), which agrees that this EPL1 expressing line is the most resistant to the bacteria ( Figure 2). In addition, we selected the EPL1-3 line for trypan blue staining after inoculation with B. cinerea ( Figure 6). We observed that the blue staining was lower in the EPL1-3 line, while a strong signal was observed in the WT leaves ( Figure 6). This confirms a decrease in plant cell death that correlates with a smaller lesion size in the transgenic EPL1-3 line.
Plants 2023, 12, 2443 8 o agrees that this EPL1 expressing line is the most resistant to the bacteria (Figure 2) addition, we selected the EPL1-3 line for trypan blue staining after inoculation with cinerea ( Figure 6). We observed that the blue staining was lower in the EPL1-3 line, w a strong signal was observed in the WT leaves ( Figure 6). This confirms a decrease in p cell death that correlates with a smaller lesion size in the transgenic EPL1-3 line.

Expression Changes Plant Defense Genes in the 35S::TaEPL1-3 Line under Botrytis cinerea Infection
We analyzed the expression levels of the LOX3 and PDF1.2 genes involved in JA biosynthesis and response, respectively, WRKY33 gene, which is a transcription factor implicated in plant defense against necrotrophic pathogens [31], and also ZAT1.

Expression Changes Plant Defense Genes in the 35S::TaEPL1-3 Line under Botrytis cinerea Infection
We analyzed the expression levels of the LOX3 and PDF1.2 genes involved in JA biosynthesis and response, respectively, WRKY33 gene, which is a transcription factor implicated in plant defense against necrotrophic pathogens [31], and also ZAT1.

Expression Changes Plant Defense Genes in the 35S::TaEPL1-3 Line under Botrytis cinerea Infection
We analyzed the expression levels of the LOX3 and PDF1.2 genes involved in JA biosynthesis and response, respectively, WRKY33 gene, which is a transcription factor implicated in plant defense against necrotrophic pathogens [31], and also ZAT1.

Determination of H2O2 Content in Arabidopsis WT and 35S::TaEPL1-3 Lines under B. cinerea and P. syringae Infection
We analyzed the H2O2 accumulation in the 15 day-old WT and 35S::TaEPL1-3 plantlets under B. cinerea and P. syringae infection at 24 and 48 hpi ( Figure 8). As observed in Figure 3, the 35S::TaEPL1-3 line accumulates a higher amount of ROS than WT under mock conditions (Figure 8). Despite the WT line showing an increase in H2O2 levels during bacterial infection at both 24 and 48 hpi, the 35S::TaEPL1-3 line exhibited the highest H2O2 accumulation (Figure 8). During B. cinerea infection, a decrease in H2O2 levels was observed at 24 and 48 hpi in the 35S::TaEPL1-3 line compared to their respective mock controls, whereas in the WT ecotype, there was a reduction in H2O2 levels at 24 hpi followed by an increase at 48 hpi compared to their mock controls ( Figure 8).  (Bc). The data show the means ± SE from three biological replicates (n = 3). The variance analysis was done by Two-way ANOVA (genotype x infection), and the letters indicate statistically significant differences by Tukey's test at p < 0.05.

Discussion
Trichoderma species are widely used in agriculture as a beneficial fungus due to its biocontrol activity and promotion of plant growth [1,32]. In the plant-Trichoderma interaction, both organisms secrete molecules for their recognition, initiating a molecular communication to achieve symbiosis between them [12,[33][34][35][36][37][38][39]. Among the molecules reported to be secreted by Trichoderma are elicitors, such as Eliciting Plant Response-Like 1 (Epl1), which is produced and secreted by T. atroviride. There is evidence of a protective role of Epl1 protein from T. atroviride against foliar maize pathogen C. graminicola [21], and also strains of T. atroviride that overexpress TaEPL1 gene increased resistance in tomato against A. solani, B. cinerea, and P. syringae infection [20]. Although the generation of Arabidopsis and rice plants expressing the SM1 elicitor of the phytopathogenic fungus M. oryzae has been reported, the effect of the expression in A. thaliana of an elicitor as EPL1 from beneficial fungus like Trichoderma and its resistance against pathogens has not been studied.
We describe here the response of A. thaliana plants that express the EPL1 elicitor to the infection by two phytopathogenic microorganisms. One of these pathogens was the hemibiotrophic P. syringae, in which A. thaliana 35S::TaEPL1 lines were more resistant to

Discussion
Trichoderma species are widely used in agriculture as a beneficial fungus due to its biocontrol activity and promotion of plant growth [1,32]. In the plant-Trichoderma interaction, both organisms secrete molecules for their recognition, initiating a molecular communication to achieve symbiosis between them [12,[33][34][35][36][37][38][39]. Among the molecules reported to be secreted by Trichoderma are elicitors, such as Eliciting Plant Response-Like 1 (Epl1), which is produced and secreted by T. atroviride. There is evidence of a protective role of Epl1 protein from T. atroviride against foliar maize pathogen C. graminicola [21], and also strains of T. atroviride that overexpress TaEPL1 gene increased resistance in tomato against A. solani, B. cinerea, and P. syringae infection [20]. Although the generation of Arabidopsis and rice plants expressing the SM1 elicitor of the phytopathogenic fungus M. oryzae has been reported, the effect of the expression in A. thaliana of an elicitor as EPL1 from beneficial fungus like Trichoderma and its resistance against pathogens has not been studied.
We describe here the response of A. thaliana plants that express the EPL1 elicitor to the infection by two phytopathogenic microorganisms. One of these pathogens was the hemibiotrophic P. syringae, in which A. thaliana 35S::TaEPL1 lines were more resistant to bacterial infection. These Arabidopsis 35S::TaEPL1 lines exhibited less damage caused by infection as well as lower bacterial load per leaf compared to the WT plants. This suggests that the EPL1 elicitor activates a defense response within the plant cells, thus generating a lower susceptibility to P. syringae.
We report that the expression of the EPL1 elicitor in Arabidopsis triggers H 2 O 2 accumulation even in the absence of pathogen inoculation. Furthermore, despite applying CAT, a H 2 O 2 scavenger, it did not completely reduce the H 2 O 2 -associated fluorescence signal in root tips of the transgenic lines, as observed in the WT. In this sense, the 35S::TaEPL1-3 line showed a higher ZAT1.2 transcript level due to the infection of the bacterium as well as in the control plants (mock), which correlates with ROS accumulation in these transgenic lines. The ZAT1.2 gene encoding a zinc finger protein is involved in oxidative stress responses [40]. After inoculation with P. syringae, the TaEPL1-3 expression line had a higher accumulation of PAL1, PR1, and WRKY54 transcripts, which are involved in the salicylic acid (SA) biosynthesis, response, and regulation, respectively. An interesting fact is that the highest accumulation of H 2 O 2 was observed in the 35S::TaEPL1-3 line after bacterial infection, both at 24 and 48 hpi. The accumulation of ROS and the induction of these SA response genes could explain the resistance shown by the 35S::TaEPL1 lines to infection by Pseudomonas. It was previously reported that the plant protective activity of SM1 and other CP proteins are associated with the accumulation of ROS and phytoalexins [26,41,42].
Likewise, we found that the expression of the TaEPL1 gene in Arabidopsis confers enhanced resistance against B. cinerea infection. The Arabidopsis 35S::TaEPL1 plants showed a smaller lesion area and a reduction of leaf cell death caused by the fungus compared to the WT plants. This is consistent with a higher expression of the WRKY33 gene in the 35S::TaEPL1 line upon infection with the fungus B. cinerea. The WRKY33 transcription factor has been reported to be involved in defense responses against B. cinerea and other necrotrophic pathogens [31]. TaEPL1 expression in Arabidopsis reduces fungal colonization of plant tissue, thus making them more resistant to this phytopathogen. An interesting observation was that the LOX3, PDF1.2, WRKY33, and ZAT1.2 genes were up-regulated in the 35S::TaEPL1-3 line under mock conditions (i.e., no fungal infection), which could suggest that EPL1 expression in the plant causes a priming of genes involved in plant defense against this necrotrophic pathogen. An important finding was that a reduction in H 2 O 2 levels was observed in the 35S::TaEPL1-3 line during infection with B. cinerea at 24 and 48 hpi compared to the mock controls. This behavior suggests that the EPL1 expressing line is actively regulating its ROS levels in response to the presence of this necrotrophic pathogen.
Our data provide evidence that TaEPL1 expressed in Arabidopsis plants can activate defense responses against pathogens such as P. syringae and B. cinerea, which activate the SA-and JA/ET pathways. An ortholog to EPL1 exists in M. oryzae called MoSM1 [27]. Expression of MoSM1 in rice significantly increased SA and JA content, and also induced SA-and JA-related biosynthesis genes and signaling under normal growth conditions [27]. In addition, the heterologous expression of the MgSM1 in A. thaliana plants confers a broad-spectrum resistance against B. cinerea, Alternaria brassicicola, and P. syringae [26]. In the MgSM1-expressing plants, some defense genes such as PR1, PR5, and PDF1.2 were upregulated, and an accumulation of ROS was reported [26]. The authors changed the 35S viral promoter to an inducible promoter, since large amounts of MgSM1 protein caused a hypersensitive response in Arabidopsis, while this phenotype has not been reported in the case of T. virens SM1 purified protein [18], nor in our study here with TaEPL1. We did not observe a visible hypersensitive response phenotype in any 35S::TaEPL1 lines. This could be due to the fact that M. grisea is a phytopathogenic fungus that causes severe disease in rice and other grasses, and the constitute expression of SM1 in planta generated a hypersensitive response with a greater accumulation of ROS, while species of the genus Trichoderma are plant symbionts, and so the higher expression of EPL1 did not generate any negative phenotype in the plant. In addition to SM1 from M. grisea, phytotoxic effects have been reported for cerato-platanin, such as BcSpl1 from B. cinerea [24], a CP of 12.4 kDa from Ceratocystis fimbriata f. sp. platani [28].
On the other hand, Arabidopsis plants carrying the 35S::TaEPL1 construct exhibited increased biomass, with the TaEPL1-4 line showing the most pronounced accelerated growth. It is noteworthy that expressing a fungal elicitor, such as EPL1 (from the strain T. atroviride IMI 206040), in Arabidopsis results in the generation of more vigorous plants. Several species of Trichoderma have been reported to exert beneficial effects on plant growth [11]. In particular, the plant growth-promoting effect has been tested using the strain T. atroviride (IMI 206040) in tomato plants [20] and A. thaliana plants [12,32,34]. Further studies focused on the mechanism of EPL1 gene are needed to elucidate its role in plant growth.

Plant Growth Conditions
Seeds of Arabidopsis 35S::TaEPL1 lines generated in this work and parental Col-0 (WT) were sterilized using 20% (v/v) commercial sodium hypochlorite (6% free chlorine) solution for 5 min, and washed seven times in sterile distilled water. Aseptic seeds were stratified during 48 h at 4 • C and afterwards germinated and grown on agar plates containing 0.2X MS pH 7, 0.5% (w/v) sucrose, and 0.8% (w/v) agar. Seedlings were incubated in a growth chamber with a photoperiod of 16 h (100 µmolm −2 s −1 )/8 h with a light/dark cycle at a temperature of 22 ± 1 • C until used in the experiments described above.

Generation of Arabidopsis thaliana 35S::TaEPL1 Lines
The EPL1 ORF (417 bp) was amplified from cDNA of mycelial tissue of the fungus Trichoderma atroviride with Pfu DNA Polymerase high fidelity (Thermo Scientific™, Waltham, MA, USA) using the primers: EPL1-Fw 5 ATGCAGTTCTCCAGCCTCTTCAAG3 and EPL1-Rv 5 TTAGAGGCCGCAGTTGCTCACAGC3 . The product was cloned into the pCR8/GW/TOPO vector (Invitrogen, Carlsbad, CA, USA). The entry clone was verified by sequencing and recombined into pMDC32 binary vector by the Gateway LR Clonase enzyme mix (Invitrogen, Carlsbad, CA, USA) to generate the pMDC32-EPL1 construct. The pMDC32-EPL1 was transferred into Agrobacterium tumefaciens strain GV2260 by electroporation and transformed into Arabidopsis thaliana WT (Col-0) plants by the floral dip method [43]. Transformant seeds were selected based on their capacity to grow on 0.2X Murashige and Skoog medium (MS) supplemented with hygromycin at a concentration of 50 mg/mL. Three independent T4 TaEPL1 overexpression lines were obtained and used in this work (35S::TaEPL1-2, -3 and -4).

Measurement of Fresh Weight of 35S::TaEPL1 and WT Lines
Arabidopsis thaliana seeds of the WT (Col-0) and 35S::TaEPL1 expression lines (TaEPL1-2, -3 and -4) were grown on 0.2X MS plates for 7 days. Subsequently, the seedlings were transferred to soil pots containing a mixture of Sunshine Mix #3 commercial substrate, perlite, and vermiculite (3:1:1). The pots were placed in a growth chamber with a temperature of 22 ± 1 • C with a photoperiod of 16 h (100 µmolm −2 s −1 )/8 h, light/dark. The fresh weight (g) of the aerial part of each 40-day-old plant was measured using an analytical balance. Data analysis was done from six biological replicates (n = 6).

Pathogen Inocula Preparation
Pseudomonas syringae pv. Tomato DC3000 was grown to an OD600 nm of 0.8 in Luria Bertani (LB) medium (pH 7.0) supplemented with 50 mg/mL of rifampicin at 28 • C with shaking. The culture was centrifuged at 13,000 rpm for 10 min. The cells were washed twice with 10 mM MgCl 2 and adjusted at an OD600 nm of 0.2 (1 × 10 8 CFU/mL) for use in the infection assays. Botrytis cinerea strain B05.10 was grown on Potato Dextrose Agar (PDA) in darkness at 28 • C for two weeks. Spores were collected in sterile water, filtered through glass wool to remove hyphae, and quantified in a Neubauer chamber under a Motic model BA-300 microscope with 40× magnification (Motic ® , San Antonio, TX, USA).

Arabidopsis 35S::TaEPL1 Expression Lines-Pseudomonas syringae Inoculation Assays
Fourteen-day-old 35S::TaEPL1 and WT plants were transferred to 50 cell tray inserts (4.5 cm cell diameter) containing a sterile mixture of sunshine Mix#3 commercial substrate:vermiculite:perlite (3:1:1), and plants were watered every three days. Four-week-old plants were inoculated with P. syringae (50 plants of each line). For each plant, three leaves were infected with bacteria suspension at an OD600 nm of 0.2 in 10 mM MgCl 2 using the abaxial injection method [44]. Mock inoculations of 35S::TaEPL1 and WT plants were made by infiltration with 10 mM MgCl 2 . Infiltrated plants were covered with a plastic dome to maintain humidity and incubated in a growth chamber with a photoperiod of 16 h (100 µmolm −2 s −1 )/8 h with a light/dark cycle at a temperature of 22 ± 1 • C. For infection symptom evaluation, the leaves were collected 72 h post-inoculation (hpi), photographed, and sterilized using 70% ethanol for 1 min, and then rinsed with distilled water. Five independent 0.5 cm 2 leaf disks from 35S::TaEPL1 and WT plants (n = 5) were ground in 10 mM MgCl 2 and serially diluted. Ten microliters of a 1:5 dilution was spotted onto LB agar plates containing 50 mg/mL rifampicin, and colonies were counted after 2 days growth in the dark at 28 • C [44]. All interaction assays with bacteria were done three times with similar results.

Arabidopsis 35S::TaEPL1 Expression Lines-Botrytis cinerea Inoculation Assays
Plants of 35S::TaEPL1 and WT were cultivated and prepared for inoculation as described above. Four-week-old 35S::TaEPL1 lines and WT plants were inoculated with B. cinerea (50 plants of each line, n = 50). For each plant, three leaves were infected on the adaxial surface of leaves avoiding the vascular system with 10 µL drops containing 1 × 10 6 spores. Control PDB (0.25X) was used as a mock treatment. Inoculated plants were covered with a plastic dome to maintain the humidity and placed in darkness for 72 h at a temperature of 22 ± 1 • C. After this period, the disease incidence (percentage of leaves that showed disease symptoms over the total number of inoculated leaves) and leaf lesion area using Image J software IJ 1.46r version (http://rsb.info.nih.gov/ accessed on 14 June 2023) were determined. Each assay was repeated three times with a similar result.

Plant Tissue Trypan Blue Staining after Botrytis cinerea Infection
Trypan blue dye was used to detect plant cell death after fungal infection. Infected leaves from four-week-old from A. thaliana WT and EPL1-3 plants were collected 24 hpi with 2 × 10 5 spores of B. cinerea strain B05.10. PDB medium (0.25X) spore diluent was applied as a mock inoculation (control). Leaves were stained by immersing in a staining solution (10 mg of trypan blue, 10 mL of lactic acid, 10 mL of phenol, 10 mL of glycerol, and 10 mL of distilled H 2 O) for 12 h. Leaves were cleared with consecutive washes in ethanol (98%) until chlorophyll was completely removed and photographed.

Hydrogen Peroxide (H 2 O 2 ) Detection by DCFH 2 -DA Labeling
In situ localization of reactive oxygen species (ROS) was performed in root tips of tenday-old WT and 35S::TaEPL1 lines using the fluorescent molecule 2 ,7 -dichlorodihydrofluorescein diacetate (DCFH2-DA, Sigma-Aldrich, Burlington, MA, USA), as described by [45], with some modifications. The effect of the exogenous application of catalase (CAT, Sigma Aldrich, Burlington, MA, USA) on the H 2 O 2 levels in the WT and 35S::TaEPL1 lines was also analyzed. Seedlings were placed in 24-well culture plates containing 0.2X MS liquid supplemented with 0 or 250 U/mL of CAT (dissolved in pH7 phosphate buffer) and incubated at 22 ± 1 • C in a growth chamber for 4 h with continuous light. The seedlings were then immersed in 25 µmol DCFH 2 -DA in Tris buffer (10 mM Tris, 50 mM KCl, pH 7.2) for 30 min in complete darkness. After rinsing with buffer to remove excess DCFH 2 -DA and CAT, the roots were observed and photographed with a fluorescence microscope (Zeiss Axio Imager M2; Carl Zeiss Microscopy, Pleasanton, CA, USA) at 10× magnification using excitation and emission wavelengths of 480 nm and 500-550 nm, respectively. Fifteen seedlings of WT and 35S::TaEPL1 lines were analyzed by treatment; each assay was repeated at least three times.

Determination of Hydrogen Peroxide Content with Potassium Iodide (KI)
Hydrogen peroxide content was determined according to Jungle et al., 2014. Ten-dayold plantlets of WT and 35S::TaEPL1 lines were transferred to plates containing 0.2X MS liquid supplemented with 0 or 250 U/mL of CAT and incubated at 22 ± 1 • C in a growth chamber for 4 h with continuous light. The CAT treatment was carried out to determine the reduction of the H 2 O 2 content between the WT and 35S::TaEPL1 lines. In another experiment, we measured the hydrogen peroxide content in 15-day-old A. thaliana WT and 35S::TaEPL1-3 plants following inoculation with pathogens. Plantlets were infected with 2 µL of P. syringae or B. cinerea inocula for 24 h and 48 h, and as mock treatments, MgCl 2 and PDB (0.25X) were used, respectively. Samples of 100 mg of fresh tissue from whole Arabidopsis plantlets were homogenized in an ice bath with 375 µL 0.1% (w/v) trichloroacetic acid. The mixture was centrifuged at 4 • C, 7000 rpm for 20 min; 250 µL of supernatant was transferred to a new tube and 250 µL of 10 mM potassium phosphate buffer (pH 7.0) was added, followed by 500 µL of 1 M KI. The absorbance was read at 390 nm using an Epoch-2 microplate reader (Biotek ® , Winooski, VT, USA). The H 2 O 2 content was determined using a standard curve (10,20,30,50,70, and 100 µM H 2 O 2 ). Data analysis was done from three biological replicates (n = 3).

RNA Isolation and RT-qPCR Gene Expression Analysis
Total RNA was extracted from 10-day-old seedlings of WT and 35::EPL1-2, -3, and -4 lines using Concert reagent (Invitrogen, Carlsbad, CA, USA), followed by DNAase Turbo digestion (Ambion, Austin, TX, USA) for genomic DNA removal. Total RNA concentration was calculated with an Epoch-2 microplate reader (Biotek ® Winooski, VT, USA). cDNA was synthesized using 1 µg total RNA and a SuperScript™ II Reverse Transcriptase kit (Invitrogen, Carlsbad, CA, USA). RT-qPCR was performed using the Step One Real-Time PCR Detection System (Applied Biosystems, Waltham, MA, USA). The reaction mixture was made in a volume of 10 µL containing 100 ng of cDNA, 200 nM of each primer (Supplementary Table S1), and 5 µL of Maxima SYBR Green/ROX qPCR Master Mix (2×) Thermo Scientific™ (Waltham, MA, USA). First, the relative expression of the ELP1 gene in 35S::TaEPL1 Arabidopsis lines was quantified. Then, the EPL1-3 line was selected to measure the expression levels of SA-mediated defense markers, such as PR1, PAL1, and WRKY54 and JA-mediated defense markers LOX3 and PDF1.2, WRKY33, as well ZAT1.2 (signaling ROS gene). Expression levels were quantified in rosette leaves of 28-day-old WT and 35S::TaEPL1-3 plants inoculated with B. cinerea or P. syringae for 24 h. The sequences of oligonucleotides used are included in the Supplementary Table S1. Gene expression was analyzed by the delta Ct method [46]. The AtUBQ5 gene was used as a reference. For each cDNA sample, three biological replicates (n = 3) were analyzed with their respective technical replicates.

Statistical Analysis
Results from representative experiments are shown as means ± SE. Statistical significance (p ≤ 0.05) among data were determined regarding genotypes by One-Way ANOVA or Two-Way ANOVA (genotype x infection). Tukey's post-tests were performed using the GraphPad Prism version 8.0 software (GraphPad Software, San Diego, CA, USA).

Conclusions
Our data show that Arabidopsis lines expressing the EPL1 elicitor of T. atroviride are clearly resistant to pathogens with different lifestyles and modes of nutrition, namely, the hemibiotrophic bacteria P. syringae and the necrotrophic fungus B. cinerea. We observed that the Arabidopsis EPL1-3 line, which had the highest expression of the TaEPL1 gene, resulted in greater protection against pathogens. This reduction in pathogen infection in EPL1 expressing plants could be correlated with the increase in ROS levels and the induction of SA-and JA-related genes. Therefore, EPL1 elicitor is an excellent candidate for use as a molecule that provides protection against diverse phytopathogens.

Data Availability Statement:
The data presented in this study are available in the manuscript.