Preliminary Phytochemical Profile and Bioactivity of Inga jinicuil Schltdl & Cham. ex G. Don

Several Mesoamerican cultures have used Inga jinicuil as traditional medicine for the treatment of gastrointestinal, inflammatory, and infectious issues. The aims of this contribution were to elucidate the phytochemical profile of the organic extracts from the bark and leaves of I. jinicuil and to assess the anti-inflammatory and antibacterial properties of these extracts. The preliminary chemical profile was determined by HPLC-PDA and GC-MS; the anti-inflammatory activity was evaluated with a mouse ear edema model, whereas the antibacterial activity was screened against several bacteria. The phytochemical profile of both organs (bark and leaves) of I. jinicuil led to the identification of 42 compounds, such as polyphenolic, flavonoids, triterpenes, prenol-type lipids, and aliphatic and non-aliphatic esters. This molecular diversity gave moderate anti-inflammatory activity (67.3 ± 2.0%, dichloromethane bark extract) and excellent antibacterial activity against Pseudomona aeruginosa and methicillin-resistant Sthaphylococcus aureus (MIC values of ˂3.12 and 50 µg/mL, respectively). These results contribute to the chemotaxonomic characterization and the rational use in traditional medicine of Inga jinicuil Schltdl & Cham. ex G. Don.


Introduction
Anti-inflammatory and antimicrobial properties have been attributed to a great diversity of plants used in traditional medicine, from which many commercial drugs have been developed [1]. These properties have been related to the presence of secondary metabolites such as tannins, terpenes, and flavonoids, among many others [2]. Currently, medicinal plants are a valuable alternative and, in agreement with the WHO strategies on complementary and traditional medicine, it is necessary to perform studies aimed at identifying their bioactive compounds and confirming their pharmacological activity in order to guarantee their safe, effective, and rational use [3].
Even though there are several alternatives for the treatment of inflammation, some antiinflammatory drugs, such as aspirin, ketorolac, naproxen, or piroxicam, have adverse effects (e.g., the risk of developing intestinal bleeding) [2]; therefore, a constant search for new HPLC analysis helped to determine the presence of terpenic and flavonoid-type compounds in both the dichloromethane and hydroalcoholic extracts from I. jinicuil. The chromatograms of the four polar extracts (Ij-LD, Ij-LHac, Ij-BD, and Ij-BHac) are presented in Figure 1. A total of 21 peaks related to terpenic and flavonoid-type compounds were observed. Table 2 presents a summary of the following information: retention times, main absorption bands of the UV-Vis spectra, and the presence of each peak in the four extracts analyzed.

HPLC and UV-Vis Spectra Analysis of Polar Extracts from Inga jinicuil
HPLC analysis helped to determine the presence of terpenic and flavonoid-type compounds in both the dichloromethane and hydroalcoholic extracts from I. jinicuil. The chromatograms of the four polar extracts (Ij-LD, Ij-LHac, Ij-BD, and Ij-BHac) are presented in Figure 1. A total of 21 peaks related to terpenic and flavonoid-type compounds were observed. Table 2 presents a summary of the following information: retention times, main absorption bands of the UV-Vis spectra, and the presence of each peak in the four extracts analyzed.   As seen in Figure 1, the peaks in the chromatograms can be differentiated into two main groups based on their retention time (t R ): the first group consists of 14 peaks with t R between 8 and 12 min, while the second group has 7 peaks with t R from 26 to 29 min.
Based on the retention times and the absorption bands in the UV-Vis spectra (Figures S1-S4) of the peaks shown in Table 2, it was possible to perform a preliminary analysis of each of the metabolites present in the extracts by comparing those parameters with known standards and data from the literature. Accordingly, for Peak 1, the observed absorption bands at 220.4, 261.6, and 294.7 nm were equal with those of the protocatechuic acid standard, which when analyzed in identical chromatographic conditions presented the same t R (8.46 min). Since Peak 2 showed chromatographic behavior similar to 1 along with the analysis of the UV-Vis bands and the literature, it is inferred that it may be a derivative of protocatechuic acid [15,16].
Regarding Peaks 3 and 7, when their t R and UV-Vis absorption bands were compared with the gallic acid standard (t R = 7.46 min, λ max = 220.4, 272.2 nm), a good match was found in the UV-Vis spectrum; however, the differences in retention times suggested the presence of gallic acid derivatives [17,18].
As seen in Figure 1, the peaks in the chromatograms can be differentiated into two main groups based on their retention time (tR): the first group consists of 14 peaks with tR between 8 and 12 min, while the second group has 7 peaks with tR from 26 to 29 min.
Based on the retention times and the absorption bands in the UV-Vis spectra (Figures S1-S4) of the peaks shown in Table 2, it was possible to perform a preliminary analysis of each of the metabolites present in the extracts by comparing those parameters with known standards and data from the literature. Accordingly, for Peak 1, the observed absorption bands at 220.4, 261.6, and 294.7 nm were equal with those of the protocatechuic acid standard, which when analyzed in identical chromatographic conditions presented the same tR (8.46 min). Since Peak 2 showed chromatographic behavior similar to 1 along with the analysis of the UV-Vis bands and the literature, it is inferred that it may be a derivative of protocatechuic acid [15,16].
Regarding Peaks 3 and 7, when their tR and UV-Vis absorption bands were compared with the gallic acid standard (tR = 7.46 min, λmax = 220.4, 272.2 nm), a good match was found in the UV-Vis spectrum; however, the differences in retention times suggested the presence of gallic acid derivatives [17,18].
The analysis of the UV-Vis bands for Peaks 4, 6, and 9 indicated that these compounds may be of the flavonoid type; this inference was strengthened when they were compared with an apigenin standard (tR = 16.76 min, λmax = 211, 267.5, 338.6 nm) and an identical match was found in their UV-Vis spectra. The differences in retention times led us to potentially consider these peaks as glycosylated analogs of this flavone [19,20].        * Extracts: Bark extracts, • (Ij-BD), ■ (Ij-BHac); Leaf extracts, ○ (Ij-LD), □ (Ij-LHac). ** Compounds were suggested by a preliminary comparison of retention time (tR) and UV-Vis bands with standards and literature data.
As seen in Figure 1, the peaks in the chromatograms can be differentiated into two main groups based on their retention time (tR): the first group consists of 14 peaks with tR between 8 and 12 min, while the second group has 7 peaks with tR from 26 to 29 min. Based on the retention times and the absorption bands in the UV-Vis spectra (Figures S1-S4) of the peaks shown in Table 2, it was possible to perform a preliminary analysis of each of the metabolites present in the extracts by comparing those parameters with known standards and data from the literature. Accordingly, for Peak 1, the observed absorption bands at 220.4, 261.6, and 294.7 nm were equal with those of the protocatechuic acid standard, which when analyzed in identical chromatographic conditions presented the same tR (8.46 min). Since Peak 2 showed chromatographic behavior similar to 1 along with the analysis of the UV-Vis bands and the literature, it is inferred that it may be a derivative of protocatechuic acid [15,16].
Regarding Peaks 3 and 7, when their tR and UV-Vis absorption bands were compared with the gallic acid standard (tR = 7.46 min, λmax = 220.4, 272.2 nm), a good match was found in the UV-Vis spectrum; however, the differences in retention times suggested the presence of gallic acid derivatives [17,18].
The analysis of the UV-Vis bands for Peaks 4, 6, and 9 indicated that these compounds may be of the flavonoid type; this inference was strengthened when they were compared with an apigenin standard (tR = 16.76 min, λmax = 211, 267.5, 338.6 nm) and an identical match was found in their UV-Vis spectra. The differences in retention times led     [19,20] analyzed.  * Extracts: Bark extracts, • (Ij-BD), ■ (Ij-BHac); Leaf extracts, ○ (Ij-LD), □ (Ij-LHac). ** Compounds were suggested by a preliminary comparison of retention time (tR) and UV-Vis bands with standards and literature data.
As seen in Figure 1, the peaks in the chromatograms can be differentiated into two main groups based on their retention time (tR): the first group consists of 14 peaks with tR between 8 and 12 min, while the second group has 7 peaks with tR from 26 to 29 min. Based on the retention times and the absorption bands in the UV-Vis spectra (Figures S1-S4) of the peaks shown in Table 2, it was possible to perform a preliminary analysis of each of the metabolites present in the extracts by comparing those parameters with known standards and data from the literature. Accordingly, for Peak 1, the observed absorption bands at 220.4, 261.6, and 294.7 nm were equal with those of the protocatechuic acid standard, which when analyzed in identical chromatographic conditions presented the same tR (8.46 min). Since Peak 2 showed chromatographic behavior similar to 1 along with the analysis of the UV-Vis bands and the literature, it is inferred that it may be a derivative of protocatechuic acid [15,16].
Regarding Peaks 3 and 7, when their tR and UV-Vis absorption bands were compared with the gallic acid standard (tR = 7.46 min, λmax = 220.4, 272.2 nm), a good match was found in the UV-Vis spectrum; however, the differences in retention times suggested the presence of gallic acid derivatives [17,18].
The analysis of the UV-Vis bands for Peaks 4, 6, and 9 indicated that these compounds may be of the flavonoid type; this inference was strengthened when they were compared with an apigenin standard (tR = 16.76 min, λmax = 211, 267.5, 338.6 nm) and an     [19,20] absorption bands of the UV-Vis spectra, and the presence of each peak in the four extracts analyzed.  * Extracts: Bark extracts, • (Ij-BD), ■ (Ij-BHac); Leaf extracts, ○ (Ij-LD), □ (Ij-LHac). ** Compounds were suggested by a preliminary comparison of retention time (tR) and UV-Vis bands with standards and literature data.
As seen in Figure 1, the peaks in the chromatograms can be differentiated into two main groups based on their retention time (tR): the first group consists of 14 peaks with tR between 8 and 12 min, while the second group has 7 peaks with tR from 26 to 29 min. Based on the retention times and the absorption bands in the UV-Vis spectra (Figures S1-S4) of the peaks shown in Table 2, it was possible to perform a preliminary analysis of each of the metabolites present in the extracts by comparing those parameters with known standards and data from the literature. Accordingly, for Peak 1, the observed absorption bands at 220.4, 261.6, and 294.7 nm were equal with those of the protocatechuic acid standard, which when analyzed in identical chromatographic conditions presented the same tR (8.46 min). Since Peak 2 showed chromatographic behavior similar to 1 along with the analysis of the UV-Vis bands and the literature, it is inferred that it may be a derivative of protocatechuic acid [15,16].
Regarding Peaks 3 and 7, when their tR and UV-Vis absorption bands were compared with the gallic acid standard (tR = 7.46 min, λmax = 220.4, 272.2 nm), a good match was found in the UV-Vis spectrum; however, the differences in retention times suggested the presence of gallic acid derivatives [17,18].
The analysis of the UV-Vis bands for Peaks 4, 6, and 9 indicated that these compounds may be of the flavonoid type; this inference was strengthened when they were  * Extracts: Bark extracts, • (Ij-BD), ■ (Ij-BHac); Leaf extracts, ○ (Ij-LD), □ (Ij-LHac). ** Compounds were suggested by a preliminary comparison of retention time (tR) and UV-Vis bands with standards and literature data.
As seen in Figure 1, the peaks in the chromatograms can be differentiated into two main groups based on their retention time (tR): the first group consists of 14 peaks with tR between 8 and 12 min, while the second group has 7 peaks with tR from 26 to 29 min. Based on the retention times and the absorption bands in the UV-Vis spectra (Figures S1-S4) of the peaks shown in Table 2, it was possible to perform a preliminary analysis of each of the metabolites present in the extracts by comparing those parameters with known standards and data from the literature. Accordingly, for Peak 1, the observed absorption bands at 220.4, 261.6, and 294.7 nm were equal with those of the protocatechuic acid standard, which when analyzed in identical chromatographic conditions presented the same tR (8.46 min). Since Peak 2 showed chromatographic behavior similar to 1 along with the analysis of the UV-Vis bands and the literature, it is inferred that it may be a derivative of protocatechuic acid [15,16].
Regarding Peaks 3 and 7, when their tR and UV-Vis absorption bands were compared with the gallic acid standard (tR = 7.46 min, λmax = 220.4, 272.2 nm), a good match was found in the UV-Vis spectrum; however, the differences in retention times suggested the presence of gallic acid derivatives [17,18].
As seen in Figure 1, the peaks in the chromatograms can be differentiated into two main groups based on their retention time (tR): the first group consists of 14 peaks with tR between 8 and 12 min, while the second group has 7 peaks with tR from 26 to 29 min. Based on the retention times and the absorption bands in the UV-Vis spectra (Figures S1-S4) of the peaks shown in Table 2, it was possible to perform a preliminary analysis of each of the metabolites present in the extracts by comparing those parameters with known standards and data from the literature. Accordingly, for Peak 1, the observed absorption bands at 220.4, 261.6, and 294.7 nm were equal with those of the protocatechuic acid standard, which when analyzed in identical chromatographic conditions presented the same tR (8.46 min). Since Peak 2 showed chromatographic behavior similar to 1 along with the analysis of the UV-Vis bands and the literature, it is inferred that it may be a derivative of protocatechuic acid [15,16].
Regarding Peaks 3 and 7, when their tR and UV-Vis absorption bands were compared with the gallic acid standard (tR = 7.46 min, λmax = 220.4, 272.2 nm), a good match was found in the UV-Vis spectrum; however, the differences in retention times suggested the presence of gallic acid derivatives [17,18].
The analysis of the UV-Vis bands for Peaks 4, 6, and 9 indicated that these com-   [19,20] absorption bands of the UV-Vis spectra, and the presence of each peak in the four extracts analyzed.    * Extracts: Bark extracts, • (Ij-BD), ■ (Ij-BHac); Leaf extracts, ○ (Ij-LD), □ (Ij-LHac). ** Compounds were suggested by a preliminary comparison of retention time (tR) and UV-Vis bands with standards and literature data.
As seen in Figure 1, the peaks in the chromatograms can be differentiated into two main groups based on their retention time (tR): the first group consists of 14 peaks with tR between 8 and 12 min, while the second group has 7 peaks with tR from 26 to 29 min. Based on the retention times and the absorption bands in the UV-Vis spectra (Figures S1-S4) of the peaks shown in Table 2, it was possible to perform a preliminary analysis of each of the metabolites present in the extracts by comparing those parameters with known standards and data from the literature. Accordingly, for Peak 1, the observed absorption bands at 220.4, 261.6, and 294.7 nm were equal with those of the protocatechuic acid standard, which when analyzed in identical chromatographic conditions presented the same tR (8.46 min). Since Peak 2 showed chromatographic behavior similar to 1 along with the analysis of the UV-Vis bands and the literature, it is inferred that it may be a derivative of protocatechuic acid [15,16].
Regarding Peaks 3 and 7, when their tR and UV-Vis absorption bands were compared with the gallic acid standard (tR = 7.46 min, λmax = 220.4, 272.2 nm), a good match was found in the UV-Vis spectrum; however, the differences in retention times suggested the pres-   [19,20] in Figure 1. A total of 21 peaks related to terpenic and flavonoid-type compounds were observed. Table 2 presents a summary of the following information: retention times, main absorption bands of the UV-Vis spectra, and the presence of each peak in the four extracts analyzed.   [19,20] pounds in both the dichloromethane and hydroalcoholic extracts from I. jinicuil. The chromatograms of the four polar extracts (Ij-LD, Ij-LHac, Ij-BD, and Ij-BHac) are presented in Figure 1. A total of 21 peaks related to terpenic and flavonoid-type compounds were observed. Table 2 presents a summary of the following information: retention times, main absorption bands of the UV-Vis spectra, and the presence of each peak in the four extracts analyzed.  matograms of the four polar extracts (Ij-LD, Ij-LHac, Ij-BD, and Ij-BHac) are presented in Figure 1. A total of 21 peaks related to terpenic and flavonoid-type compounds were observed. Table 2 presents a summary of the following information: retention times, main absorption bands of the UV-Vis spectra, and the presence of each peak in the four extracts analyzed.  pounds in both the dichloromethane and hydroalcoholic extracts from I. jinicuil. The chromatograms of the four polar extracts (Ij-LD, Ij-LHac, Ij-BD, and Ij-BHac) are presented in Figure 1. A total of 21 peaks related to terpenic and flavonoid-type compounds were observed. Table 2 presents a summary of the following information: retention times, main absorption bands of the UV-Vis spectra, and the presence of each peak in the four extracts analyzed.  * Extracts: Bark extracts, • (Ij-BD), ■ (Ij-BHac); Leaf extracts, ○ (Ij-LD), □ (Ij-LHac). ** Compounds were suggested by a preliminary comparison of retention time (tR) and UV-Vis bands with standards and literature data.
As seen in Figure 1, the peaks in the chromatograms can be differentiated into two main groups based on their retention time (tR): the first group consists of 14 peaks with tR between 8 and 12 min, while the second group has 7 peaks with tR from 26 to 29 min. Based on the retention times and the absorption bands in the UV-Vis spectra (Figures S1-S4) of the peaks shown in Table 2, it was possible to perform a preliminary analysis of each of the metabolites present in the extracts by comparing those parameters with known standards and data from the literature. Accordingly, for Peak 1, the observed absorption bands at 220.4, 261.6, and 294.7 nm were equal with those of the protocatechuic acid standard, which when analyzed in identical chromatographic conditions presented the same tR (8.46 min). Since Peak 2 showed chromatographic behavior similar to 1 along with the analysis of the UV-Vis bands and the literature, it is inferred that it may be a derivative of protocatechuic acid [15,16]. absorption bands of the UV-Vis spectra, and the presence of each peak in the four extracts analyzed.   [19,20] HPLC analysis helped to determine the presence of terpenic and flavonoid-type compounds in both the dichloromethane and hydroalcoholic extracts from I. jinicuil. The chromatograms of the four polar extracts (Ij-LD, Ij-LHac, Ij-BD, and Ij-BHac) are presented in Figure 1. A total of 21 peaks related to terpenic and flavonoid-type compounds were observed. Table 2 presents a summary of the following information: retention times, main absorption bands of the UV-Vis spectra, and the presence of each peak in the four extracts analyzed.

HPLC and UV-Vis Spectra Analysis of Polar Extracts from Inga jinicuil
HPLC analysis helped to determine the presence of terpenic and flavonoid-type compounds in both the dichloromethane and hydroalcoholic extracts from I. jinicuil. The chromatograms of the four polar extracts (Ij-LD, Ij-LHac, Ij-BD, and Ij-BHac) are presented in Figure 1. A total of 21 peaks related to terpenic and flavonoid-type compounds were observed. Table 2 presents a summary of the following information: retention times, main absorption bands of the UV-Vis spectra, and the presence of each peak in the four extracts analyzed.  * Extracts: Bark extracts, • (Ij-BD), ■ (Ij-BHac); Leaf extracts, ○ (Ij-LD), □ (Ij-LHac). ** Compounds were suggested by a preliminary comparison of retention time (tR) and UV-Vis bands with standards and literature data.
As seen in Figure 1, the peaks in the chromatograms can be differentiated into two main groups based on their retention time (tR): the first group consists of 14 peaks with tR between 8 and 12 min, while the second group has 7 peaks with tR from 26 to 29 min. Based on the retention times and the absorption bands in the UV-Vis spectra (Figures S1-S4) of the peaks shown in Table 2, it was possible to perform a preliminary analysis of each of the metabolites present in the extracts by comparing those parameters with known standards and data from the literature. Accordingly, for Peak 1, the observed absorption bands at 220.4, 261.6, and 294.7 nm were equal with those of the protocatechuic acid standard, which when analyzed in identical chromatographic conditions presented the same tR (8.46 min). Since Peak 2 showed chromatographic behavior similar to 1 along with the analysis of the UV-Vis bands and the literature, it is inferred that it may be a derivative of protocatechuic acid [15,16]. observed. Table 2 presents a summary of the following information: retention times, main absorption bands of the UV-Vis spectra, and the presence of each peak in the four extracts analyzed.   [19,20] HPLC analysis helped to determine the presence of terpenic and flavonoid-type compounds in both the dichloromethane and hydroalcoholic extracts from I. jinicuil. The chromatograms of the four polar extracts (Ij-LD, Ij-LHac, Ij-BD, and Ij-BHac) are presented in Figure 1. A total of 21 peaks related to terpenic and flavonoid-type compounds were observed. Table 2 presents a summary of the following information: retention times, main absorption bands of the UV-Vis spectra, and the presence of each peak in the four extracts analyzed.

HPLC and UV-Vis Spectra Analysis of Polar Extracts from Inga jinicuil
HPLC analysis helped to determine the presence of terpenic and flavonoid-type compounds in both the dichloromethane and hydroalcoholic extracts from I. jinicuil. The chromatograms of the four polar extracts (Ij-LD, Ij-LHac, Ij-BD, and Ij-BHac) are presented in Figure 1. A total of 21 peaks related to terpenic and flavonoid-type compounds were observed. Table 2 presents a summary of the following information: retention times, main absorption bands of the UV-Vis spectra, and the presence of each peak in the four extracts analyzed.  * Extracts: Bark extracts, • (Ij-BD), ■ (Ij-BHac); Leaf extracts, ○ (Ij-LD), □ (Ij-LHac). ** Compounds were suggested by a preliminary comparison of retention time (tR) and UV-Vis bands with standards and literature data.
As seen in Figure 1, the peaks in the chromatograms can be differentiated into two main groups based on their retention time (tR): the first group consists of 14 peaks with tR between 8 and 12 min, while the second group has 7 peaks with tR from 26 to 29 min. Based on the retention times and the absorption bands in the UV-Vis spectra (Figures S1-S4) of the peaks shown in Table 2, it was possible to perform a preliminary analysis of each of the metabolites present in the extracts by comparing those parameters with known standards and data from the literature. Accordingly, for Peak 1, the observed absorption bands at 220.4, 261.6, and 294.7 nm were equal with those of the protocatechuic acid standard, which when analyzed in identical chromatographic conditions presented the same tR (8.46 min). Since Peak 2 showed chromatographic behavior similar to 1 along with the analysis of the UV-Vis bands and the literature, it is inferred that it may be a derivative of in Figure 1. A total of 21 peaks related to terpenic and flavonoid-type compounds were observed. Table 2 presents a summary of the following information: retention times, main absorption bands of the UV-Vis spectra, and the presence of each peak in the four extracts analyzed.

HPLC and UV-Vis Spectra Analysis of Polar Extracts from Inga jinicuil
HPLC analysis helped to determine the presence of terpenic and flavonoid-type compounds in both the dichloromethane and hydroalcoholic extracts from I. jinicuil. The chromatograms of the four polar extracts (Ij-LD, Ij-LHac, Ij-BD, and Ij-BHac) are presented in Figure 1. A total of 21 peaks related to terpenic and flavonoid-type compounds were observed. Table 2 presents a summary of the following information: retention times, main absorption bands of the UV-Vis spectra, and the presence of each peak in the four extracts analyzed.

HPLC and UV-Vis Spectra Analysis of Polar Extracts from Inga jinicuil
HPLC analysis helped to determine the presence of terpenic and flavonoid-type compounds in both the dichloromethane and hydroalcoholic extracts from I. jinicuil. The chromatograms of the four polar extracts (Ij-LD, Ij-LHac, Ij-BD, and Ij-BHac) are presented in Figure 1. A total of 21 peaks related to terpenic and flavonoid-type compounds were observed. Table 2 presents a summary of the following information: retention times, main absorption bands of the UV-Vis spectra, and the presence of each peak in the four extracts analyzed.

HPLC and UV-Vis Spectra Analysis of Polar Extracts from Inga jinicuil
HPLC analysis helped to determine the presence of terpenic and flavonoid-type compounds in both the dichloromethane and hydroalcoholic extracts from I. jinicuil. The chromatograms of the four polar extracts (Ij-LD, Ij-LHac, Ij-BD, and Ij-BHac) are presented in Figure 1. A total of 21 peaks related to terpenic and flavonoid-type compounds were observed. Table 2 presents a summary of the following information: retention times, main absorption bands of the UV-Vis spectra, and the presence of each peak in the four extracts analyzed.

HPLC and UV-Vis Spectra Analysis of Polar Extracts from Inga jinicuil
HPLC analysis helped to determine the presence of terpenic and flavonoid-type compounds in both the dichloromethane and hydroalcoholic extracts from I. jinicuil. The chromatograms of the four polar extracts (Ij-LD, Ij-LHac, Ij-BD, and Ij-BHac) are presented in Figure 1. A total of 21 peaks related to terpenic and flavonoid-type compounds were observed. Table 2 presents a summary of the following information: retention times, main absorption bands of the UV-Vis spectra, and the presence of each peak in the four extracts analyzed.  * Extracts: Bark extracts, • (Ij-BD), ■ (Ij-BHac); Leaf extracts, ○ (Ij-LD), □ (Ij-LHac). ** Compounds were suggested by a preliminary comparison of retention time (tR) and UV-Vis bands with standards and literature data.
As seen in Figure 1, the peaks in the chromatograms can be differentiated into two main groups based on their retention time (tR): the first group consists of 14 peaks with tR between 8 and 12 min, while the second group has 7 peaks with tR from 26 to 29 min. Based on the retention times and the absorption bands in the UV-Vis spectra ( Figures  S1-S4) of the peaks shown in Table 2, it was possible to perform a preliminary analysis of each of the metabolites present in the extracts by comparing those parameters with known standards and data from the literature. Accordingly, for Peak 1, the observed absorption bands at 220.4, 261.6, and 294.7 nm were equal with those of the protocatechuic acid standard, which when analyzed in identical chromatographic conditions presented the same tR (8.46 min). Since Peak 2 showed chromatographic behavior similar to 1 along with the (Ij-LD), matograms of the four polar extracts (Ij-LD, Ij-LHac, Ij-BD, and Ij-BHac) are presente in Figure 1. A total of 21 peaks related to terpenic and flavonoid-type compounds we observed. Table 2 presents a summary of the following information: retention times, ma absorption bands of the UV-Vis spectra, and the presence of each peak in the four extrac analyzed.   [19,20] (Ij-LHac). ** Compounds were suggested by a preliminary comparison of retention time (t R ) and UV-Vis bands with standards and literature data.
For the case of Peak 5, its UV-Vis pattern was comparable to those reported for lignane-type compounds. Similarly, Peak 8 presented characteristic bands associated with derivatives of coumaric acid [23]. On the other hand, for Peaks 12 and 18, their UV-Vis spectra were characteristic of those reported for coumarin-type compounds [25][26][27].
Regarding the analysis of the UV-Vis spectra of Peaks 10, 11, 14, and 16, it was possible to associate them with previous reports for terpene derivatives [24]. Likewise, Peaks 13 and 17 were consistent with bibliographical data of epigallocatechin gallate derivatives [28], and Peak 15 may be associated with vanillic acid derivatives [29,30]. Finally, the absorption bands of Peaks 19, 20, and 21 were associated with salicylate derivatives [31].
Considering the above information, Peaks 10, 11, 16, 19, 20, and 21, attributed to terpenes and salicylates, were detected in the four extracts analyzed, whereas Peak 14, which was also recognized as a terpene, was found in three extracts (absent in Ij-BHac). The two leaf extracts shared the presence of Peaks 6 and 12, which were consistent with apigenin and coumarin derivatives, respectively. Despite this, as can be appreciated in Table 2, around 60% of the metabolites identified in the polar extracts of the bark and leaves of Inga jinicuil were only found in one extract.
It should be noted that, to date, no reports have been found on secondary metabolites present in bark or leaves from I. jinicuil, so this work represents a first approach for the phytochemical study of these organs of the plant. However, there are reports about the phytochemical composition for other species of the Inga genus, where the presence of a high content of polyphenols with an important antioxidant capacity has been demonstrated [31]. For I. semialata and I. edulis, the analysis of leaf extracts allowed the identification of compounds such as: epicatechin, apigenin C-di-hexoside, myricetin-O-hexose-deoxyhexose, myricetin-O-deoxyhexose, and vicenin-2 [9,32]. Likewise, other studies on leaf extracts from I. edulis, reported the presence of four triterpenes (lupeol, α-amirin, olean-18-ene acid, and frideline), three flavonoids, eight phenolic acids, an anthocyanin derived from delphinidin-3-glycoside, and a mixture of five acylated anthocyanins. It is important to highlight the fact that gallic acid, methyl gallate, protocatechuic acid, and quercetin were also identified [33]. For I. laurina, there is a presence of flavonoids 3-O-(2"-O-galloyl)-αrhamnopyranoside and myricetin-3-rhamnoside in leaf extracts [19], as well as gallic acid, myricetin derivatives, quercetin glycoside, and glycoside myricetin-3-O-rhamnosid from ethanolic extracts of leaves from this plant [20].
In view of the above-mentioned studies, our preliminary assessment of the phytochemical profile of Inga jinicuil allowed the identification of a chemotaxonomic resemblance with other species of the same genus, since a shared presence of phenolic and terpenic compounds, such as gallates, protocatechuic acid, and its derivatives, as well as flavonoids such as apigenin, can be recognized. It should be emphasized that in published work, the phytochemical research reports on I semialata, I. eludis, and I. laurina refer mainly to polar leaf extracts, whereas the phytochemical analysis of bark has been oriented to non-polar extracts (as discussed below). Therefore, this report also contributes to the identification of secondary metabolites in polar extracts from this organ for a species of the Inga genus.

Chemical Profile of Hexane Extracts from Bark and Leaves of I. jinicuil by GC-MS
The analysis of the GC-MS chromatograms of Ij-BH and Ij-LH [ Figure 2A,B] allowed the identification of 21 compounds, where 7 of them were only found in the bark extracts, 11 compounds only appeared in the analysis of the leaf extracts, and 3 werecommon to the extracts of both organs. Table 3 presents a list of the compounds detected arranged according to their elution order. The most abundant compounds detected for Ij-BH were prenol, α-tocopherol (relative abundance: 40.49%), and triterpene 24-methylenecycloartan-3-one (38.61%); these compounds represented approximately 80% of the content of this extract. For the Ij-LH extract, the triterpenes included lup-20 (29)-en-3-one (26.74%) and lupeol (16.44%), as well as the aliphatic compound hentriacontane (16.66%), all of which constituted nearly 60% of its metabolic content. The compounds identified in both extracts were hexadecanoic acid methyl ester, hexadecanoic acid ethyl ester, and octadecanoic acid methyl ester. It is worth mentioning that these three compounds were found in greater abundance in Ij-BH.
It is worth noting that this is the first report of a CG-MS analysis of hexanic extracts from Inga jinicuil. However, similar studies have been documented for other species of the Inga genus; such is the case for I. edulis, where triterpene compounds including lupeol and stigmasterol, as well as aliphatic compounds, have been identified from extracts of the bark and leaves [33,34]. Likewise, extracts from the bark and leaves of I. laurina have shown the presence of terpenes such as phytol, the aliphatic nonacosane, and esterified aliphatic acids [35], whereas in a hexanic fraction obtained from the leaves of I. semialata, the main compounds isolated were triterpenes, such as lupeol, α-amyrin, oleanolic acid, and friedelin [30]. In this report, the presence of esterified aliphatic acids was identified and, as in other species of the Inga genus, the presence of lupeol has been established. However, the following compounds: hentriacontane, α-tocopherol, lup-20 (29)-en-3-one and 24-methylenecycloartan-3-one, are reported for the first time for this genus.
Plants 2022, 11, x 6 of 15 extracts were hexadecanoic acid methyl ester, hexadecanoic acid ethyl ester, and octadecanoic acid methyl ester. It is worth mentioning that these three compounds were found in greater abundance in Ij-BH.

Anti-Inflammatory Activity of Organic Extracts from Inga jinicuil
The results corresponding to the anti-inflammatory study of the organic extracts are presented in Figure 3. At the same dose of 1.0 mg/ear, all of the extracts showed anti-inflammatory activity. For the bark extracts, the percentages of inhibition were: Ij-BH 34.6 ± 3.0%, Ij-BD 67.3 ± 2.0%, and Ij-BHac 24.4 ±1.0%, and for leaf extracts, the corresponding percentages were: Ij-LH 34.9 ± 1.3%, Ij-LD 23.0 ± 1.0%, and Ij-LHac 49.6 ± 1.0%. For indomethacin (Indo), which was employed as the reference drug, the inhibition percentage was 75.5 ± 2.2%. As can be seen, the two extracts with the greatest anti-inflammatory activity were Ij-BD followed by Ij-LHac, and the statistical comparison between the anti-inflammatory activities of the extracts and the reference drug revealed significant differences (p < 0.05). No extract reached an effect equal to or greater than that of Indo (Indomethacin). However, the comparison using the Tukey test of the effect of the extracts and the reference drug showed that there were no significant differences (p < 0.05) between some of the extracts, such as Ij-BH compared to Ij-LH and Ij-BHac compared to Ij-LD. It is worth noting that this is the first report of a CG-MS analysis of hexanic extracts from Inga jinicuil. However, similar studies have been documented for other species of the Inga genus; such is the case for I. edulis, where triterpene compounds including lupeol and stigmasterol, as well as aliphatic compounds, have been identified from extracts of the bark and leaves [33,34]. Likewise, extracts from the bark and leaves of I. laurina have shown the presence of terpenes such as phytol, the aliphatic nonacosane, and esterified aliphatic acids [35], whereas in a hexanic fraction obtained from the leaves of I. semialata, the main compounds isolated were triterpenes, such as lupeol, α-amyrin, oleanolic acid, and friedelin [30]. In this report, the presence of esterified aliphatic acids was identified and, as in other species of the Inga genus, the presence of lupeol has been established. However, the following compounds: hentriacontane, α-tocopherol, lup-20 (29)-en-3-one and 24-methylenecycloartan-3-one, are reported for the first time for this genus.

Anti-Inflammatory Activity of Organic Extracts from Inga jinicuil
The results corresponding to the anti-inflammatory study of the organic extracts are presented in Figure 3. At the same dose of 1.0 mg/ear, all of the extracts showed antiinflammatory activity. For the bark extracts, the percentages of inhibition were: Ij-BH 34.6 ± 3.0%, Ij-BD 67.3 ± 2.0%, and Ij-BHac 24.4 ±1.0%, and for leaf extracts, the corresponding percentages were: Ij-LH 34.9 ± 1.3%, Ij-LD 23.0 ± 1.0%, and Ij-LHac 49.6 ± 1.0%. For indomethacin (Indo), which was employed as the reference drug, the inhibition percentage was 75.5 ± 2.2%. As can be seen, the two extracts with the greatest anti-inflammatory activity were Ij-BD followed by Ij-LHac, and the statistical comparison between the anti-  It is worth noting that this is the first report of a CG-MS analysis of hexanic extracts from Inga jinicuil. However, similar studies have been documented for other species of the Inga genus; such is the case for I. edulis, where triterpene compounds including lupeol and stigmasterol, as well as aliphatic compounds, have been identified from extracts of the bark and leaves [33,34]. Likewise, extracts from the bark and leaves of I. laurina have shown the presence of terpenes such as phytol, the aliphatic nonacosane, and esterified aliphatic acids [35], whereas in a hexanic fraction obtained from the leaves of I. semialata, the main compounds isolated were triterpenes, such as lupeol, α-amyrin, oleanolic acid, and friedelin [30]. In this report, the presence of esterified aliphatic acids was identified and, as in other species of the Inga genus, the presence of lupeol has been established. However, the following compounds: hentriacontane, α-tocopherol, lup-20 (29)-en-3-one and 24-methylenecycloartan-3-one, are reported for the first time for this genus.

Anti-Inflammatory Activity of Organic Extracts from Inga jinicuil
The results corresponding to the anti-inflammatory study of the organic extracts are presented in Figure 3. At the same dose of 1.0 mg/ear, all of the extracts showed antiinflammatory activity. For the bark extracts, the percentages of inhibition were: Ij-BH 34.6 ± 3.0%, Ij-BD 67.3 ± 2.0%, and Ij-BHac 24.4 ±1.0%, and for leaf extracts, the corresponding percentages were: Ij-LH 34.9 ± 1.3%, Ij-LD 23.0 ± 1.0%, and Ij-LHac 49.6 ± 1.0%. For indomethacin (Indo), which was employed as the reference drug, the inhibition percentage was 75.5 ± 2.2%. As can be seen, the two extracts with the greatest anti-inflammatory activity were Ij-BD followed by Ij-LHac, and the statistical comparison between the anti-  It is worth noting that this is the first report of a CG-MS analysis of hexanic extracts from Inga jinicuil. However, similar studies have been documented for other species of the Inga genus; such is the case for I. edulis, where triterpene compounds including lupeol and stigmasterol, as well as aliphatic compounds, have been identified from extracts of the bark and leaves [33,34]. Likewise, extracts from the bark and leaves of I. laurina have shown the presence of terpenes such as phytol, the aliphatic nonacosane, and esterified aliphatic acids [35], whereas in a hexanic fraction obtained from the leaves of I. semialata, the main compounds isolated were triterpenes, such as lupeol, α-amyrin, oleanolic acid, and friedelin [30]. In this report, the presence of esterified aliphatic acids was identified and, as in other species of the Inga genus, the presence of lupeol has been established. However, the following compounds: hentriacontane, α-tocopherol, lup-20 (29)-en-3-one and 24-methylenecycloartan-3-one, are reported for the first time for this genus.

Anti-Inflammatory Activity of Organic Extracts from Inga jinicuil
The results corresponding to the anti-inflammatory study of the organic extracts are presented in Figure 3. At the same dose of 1.0 mg/ear, all of the extracts showed antiinflammatory activity. For the bark extracts, the percentages of inhibition were: Ij-BH 34.6 ± 3.0%, Ij-BD 67.3 ± 2.0%, and Ij-BHac 24.4 ±1.0%, and for leaf extracts, the corresponding percentages were: Ij-LH 34.9 ± 1.3%, Ij-LD 23.0 ± 1.0%, and Ij-LHac 49.6 ± 1.0%. For indomethacin (Indo), which was employed as the reference drug, the inhibition percentage was 75.5 ± 2.2%. As can be seen, the two extracts with the greatest anti-inflammatory activity were Ij-BD followed by Ij-LHac, and the statistical comparison between the anti-  It is worth noting that this is the first report of a CG-MS analysis of hexanic extracts from Inga jinicuil. However, similar studies have been documented for other species of the Inga genus; such is the case for I. edulis, where triterpene compounds including lupeol and stigmasterol, as well as aliphatic compounds, have been identified from extracts of the bark and leaves [33,34]. Likewise, extracts from the bark and leaves of I. laurina have shown the presence of terpenes such as phytol, the aliphatic nonacosane, and esterified aliphatic acids [35], whereas in a hexanic fraction obtained from the leaves of I. semialata, the main compounds isolated were triterpenes, such as lupeol, α-amyrin, oleanolic acid, and friedelin [30]. In this report, the presence of esterified aliphatic acids was identified and, as in other species of the Inga genus, the presence of lupeol has been established. However, the following compounds: hentriacontane, α-tocopherol, lup-20 (29)-en-3-one and 24-methylenecycloartan-3-one, are reported for the first time for this genus.

Anti-Inflammatory Activity of Organic Extracts from Inga jinicuil
The results corresponding to the anti-inflammatory study of the organic extracts are presented in Figure 3. At the same dose of 1.0 mg/ear, all of the extracts showed antiinflammatory activity. For the bark extracts, the percentages of inhibition were: Ij-BH 34.6 ± 3.0%, Ij-BD 67.3 ± 2.0%, and Ij-BHac 24.4 ±1.0%, and for leaf extracts, the corresponding percentages were: Ij-LH 34.9 ± 1.3%, Ij-LD 23.0 ± 1.0%, and Ij-LHac 49.6 ± 1.0%. For indomethacin (Indo), which was employed as the reference drug, the inhibition percentage was 75.5 ± 2.2%. As can be seen, the two extracts with the greatest anti-inflammatory activity were Ij-BD followed by Ij-LHac, and the statistical comparison between the anti-  It is worth noting that this is the first report of a CG-MS analysis of hexanic extracts from Inga jinicuil. However, similar studies have been documented for other species of the Inga genus; such is the case for I. edulis, where triterpene compounds including lupeol and stigmasterol, as well as aliphatic compounds, have been identified from extracts of the bark and leaves [33,34]. Likewise, extracts from the bark and leaves of I. laurina have shown the presence of terpenes such as phytol, the aliphatic nonacosane, and esterified aliphatic acids [35], whereas in a hexanic fraction obtained from the leaves of I. semialata, the main compounds isolated were triterpenes, such as lupeol, α-amyrin, oleanolic acid, and friedelin [30]. In this report, the presence of esterified aliphatic acids was identified and, as in other species of the Inga genus, the presence of lupeol has been established. However, the following compounds: hentriacontane, α-tocopherol, lup-20 (29)-en-3-one and 24-methylenecycloartan-3-one, are reported for the first time for this genus.

Anti-Inflammatory Activity of Organic Extracts from Inga jinicuil
The results corresponding to the anti-inflammatory study of the organic extracts are presented in Figure 3. At the same dose of 1.0 mg/ear, all of the extracts showed antiinflammatory activity. For the bark extracts, the percentages of inhibition were: Ij-BH 34.6 ± 3.0%, Ij-BD 67.3 ± 2.0%, and Ij-BHac 24.4 ±1.0%, and for leaf extracts, the corresponding percentages were: Ij-LH 34.9 ± 1.3%, Ij-LD 23.0 ± 1.0%, and Ij-LHac 49.6 ± 1.0%. For indomethacin (Indo), which was employed as the reference drug, the inhibition percentage was 75.5 ± 2.2%. As can be seen, the two extracts with the greatest anti-inflammatory activity were Ij-BD followed by Ij-LHac, and the statistical comparison between the anti-  It is worth noting that this is the first report of a CG-MS analysis of hexanic extracts from Inga jinicuil. However, similar studies have been documented for other species of the Inga genus; such is the case for I. edulis, where triterpene compounds including lupeol and stigmasterol, as well as aliphatic compounds, have been identified from extracts of the bark and leaves [33,34]. Likewise, extracts from the bark and leaves of I. laurina have shown the presence of terpenes such as phytol, the aliphatic nonacosane, and esterified aliphatic acids [35], whereas in a hexanic fraction obtained from the leaves of I. semialata, the main compounds isolated were triterpenes, such as lupeol, α-amyrin, oleanolic acid, and friedelin [30]. In this report, the presence of esterified aliphatic acids was identified and, as in other species of the Inga genus, the presence of lupeol has been established. However, the following compounds: hentriacontane, α-tocopherol, lup-20 (29)-en-3-one and 24-methylenecycloartan-3-one, are reported for the first time for this genus.

Anti-Inflammatory Activity of Organic Extracts from Inga jinicuil
The results corresponding to the anti-inflammatory study of the organic extracts are presented in Figure 3. At the same dose of 1.0 mg/ear, all of the extracts showed antiinflammatory activity. For the bark extracts, the percentages of inhibition were: Ij-BH 34.6 ± 3.0%, Ij-BD 67.3 ± 2.0%, and Ij-BHac 24.4 ±1.0%, and for leaf extracts, the corresponding percentages were: Ij-LH 34.9 ± 1.3%, Ij-LD 23.0 ± 1.0%, and Ij-LHac 49.6 ± 1.0%. For indomethacin (Indo), which was employed as the reference drug, the inhibition percentage was 75.5 ± 2.2%. As can be seen, the two extracts with the greatest anti-inflammatory activity were Ij-BD followed by Ij-LHac, and the statistical comparison between the anti-  It is worth noting that this is the first report of a CG-MS analysis of hexanic extracts from Inga jinicuil. However, similar studies have been documented for other species of the Inga genus; such is the case for I. edulis, where triterpene compounds including lupeol and stigmasterol, as well as aliphatic compounds, have been identified from extracts of the bark and leaves [33,34]. Likewise, extracts from the bark and leaves of I. laurina have shown the presence of terpenes such as phytol, the aliphatic nonacosane, and esterified aliphatic acids [35], whereas in a hexanic fraction obtained from the leaves of I. semialata, the main compounds isolated were triterpenes, such as lupeol, α-amyrin, oleanolic acid, and friedelin [30]. In this report, the presence of esterified aliphatic acids was identified and, as in other species of the Inga genus, the presence of lupeol has been established. However, the following compounds: hentriacontane, α-tocopherol, lup-20 (29)-en-3-one and 24-methylenecycloartan-3-one, are reported for the first time for this genus.

Anti-Inflammatory Activity of Organic Extracts from Inga jinicuil
The results corresponding to the anti-inflammatory study of the organic extracts are presented in Figure 3. At the same dose of 1.0 mg/ear, all of the extracts showed antiinflammatory activity. For the bark extracts, the percentages of inhibition were: Ij-BH 34.6 ± 3.0%, Ij-BD 67.3 ± 2.0%, and Ij-BHac 24.4 ±1.0%, and for leaf extracts, the corresponding percentages were: Ij-LH 34.9 ± 1.3%, Ij-LD 23.0 ± 1.0%, and Ij-LHac 49.6 ± 1.0%. For indomethacin (Indo), which was employed as the reference drug, the inhibition percentage was 75.5 ± 2.2%. As can be seen, the two extracts with the greatest anti-inflammatory activity were Ij-BD followed by Ij-LHac, and the statistical comparison between the anti-  It is worth noting that this is the first report of a CG-MS analysis of hexanic extracts from Inga jinicuil. However, similar studies have been documented for other species of the Inga genus; such is the case for I. edulis, where triterpene compounds including lupeol and stigmasterol, as well as aliphatic compounds, have been identified from extracts of the bark and leaves [33,34]. Likewise, extracts from the bark and leaves of I. laurina have shown the presence of terpenes such as phytol, the aliphatic nonacosane, and esterified aliphatic acids [35], whereas in a hexanic fraction obtained from the leaves of I. semialata, the main compounds isolated were triterpenes, such as lupeol, α-amyrin, oleanolic acid, and friedelin [30]. In this report, the presence of esterified aliphatic acids was identified and, as in other species of the Inga genus, the presence of lupeol has been established. However, the following compounds: hentriacontane, α-tocopherol, lup-20 (29)-en-3-one and 24-methylenecycloartan-3-one, are reported for the first time for this genus.

Anti-Inflammatory Activity of Organic Extracts from Inga jinicuil
The results corresponding to the anti-inflammatory study of the organic extracts are presented in Figure 3. At the same dose of 1.0 mg/ear, all of the extracts showed antiinflammatory activity. For the bark extracts, the percentages of inhibition were: Ij-BH 34.6 ± 3.0%, Ij-BD 67.3 ± 2.0%, and Ij-BHac 24.4 ±1.0%, and for leaf extracts, the corresponding percentages were: Ij-LH 34.9 ± 1.3%, Ij-LD 23.0 ± 1.0%, and Ij-LHac 49.6 ± 1.0%. For indomethacin (Indo), which was employed as the reference drug, the inhibition percentage was 75.5 ± 2.2%. As can be seen, the two extracts with the greatest anti-inflammatory activity were Ij-BD followed by Ij-LHac, and the statistical comparison between the anti- (11. It is worth noting that this is the first report of a CG-MS analysis of hexanic extracts from Inga jinicuil. However, similar studies have been documented for other species of the Inga genus; such is the case for I. edulis, where triterpene compounds including lupeol and stigmasterol, as well as aliphatic compounds, have been identified from extracts of the bark and leaves [33,34]. Likewise, extracts from the bark and leaves of I. laurina have shown the presence of terpenes such as phytol, the aliphatic nonacosane, and esterified aliphatic acids [35], whereas in a hexanic fraction obtained from the leaves of I. semialata, the main compounds isolated were triterpenes, such as lupeol, α-amyrin, oleanolic acid, and friedelin [30]. In this report, the presence of esterified aliphatic acids was identified and, as in other species of the Inga genus, the presence of lupeol has been established. However, the following compounds: hentriacontane, α-tocopherol, lup-20 (29)-en-3-one and 24-methylenecycloartan-3-one, are reported for the first time for this genus.

Anti-Inflammatory Activity of Organic Extracts from Inga jinicuil
The results corresponding to the anti-inflammatory study of the organic extracts are presented in Figure 3. At the same dose of 1.0 mg/ear, all of the extracts showed antiinflammatory activity. For the bark extracts, the percentages of inhibition were: Ij-BH 34.6 ± 3.0%, Ij-BD 67.3 ± 2.0%, and Ij-BHac 24.4 ±1.0%, and for leaf extracts, the corresponding percentages were: Ij-LH 34.9 ± 1.3%, Ij-LD 23.0 ± 1.0%, and Ij-LHac 49.6 ± 1.0%. For indomethacin (Indo), which was employed as the reference drug, the inhibition percentage was 75.5 ± 2.2%. As can be seen, the two extracts with the greatest anti-inflammatory activity were Ij-BD followed by Ij-LHac, and the statistical comparison between the anti-  It is worth noting that this is the first report of a CG-MS analysis of hexanic extracts from Inga jinicuil. However, similar studies have been documented for other species of the Inga genus; such is the case for I. edulis, where triterpene compounds including lupeol and stigmasterol, as well as aliphatic compounds, have been identified from extracts of the bark and leaves [33,34]. Likewise, extracts from the bark and leaves of I. laurina have shown the presence of terpenes such as phytol, the aliphatic nonacosane, and esterified aliphatic acids [35], whereas in a hexanic fraction obtained from the leaves of I. semialata, the main compounds isolated were triterpenes, such as lupeol, α-amyrin, oleanolic acid, and friedelin [30]. In this report, the presence of esterified aliphatic acids was identified and, as in other species of the Inga genus, the presence of lupeol has been established. However, the following compounds: hentriacontane, α-tocopherol, lup-20 (29)-en-3-one and 24-methylenecycloartan-3-one, are reported for the first time for this genus.

Anti-Inflammatory Activity of Organic Extracts from Inga jinicuil
The results corresponding to the anti-inflammatory study of the organic extracts are presented in Figure 3. At the same dose of 1.0 mg/ear, all of the extracts showed antiinflammatory activity. For the bark extracts, the percentages of inhibition were: Ij-BH 34.6 ± 3.0%, Ij-BD 67.3 ± 2.0%, and Ij-BHac 24.4 ±1.0%, and for leaf extracts, the corresponding percentages were: Ij-LH 34.9 ± 1.3%, Ij-LD 23.0 ± 1.0%, and Ij-LHac 49.6 ± 1.0%. For indomethacin (Indo), which was employed as the reference drug, the inhibition percentage  It is worth noting that this is the first report of a CG-MS analysis of hexanic extracts from Inga jinicuil. However, similar studies have been documented for other species of the Inga genus; such is the case for I. edulis, where triterpene compounds including lupeol and stigmasterol, as well as aliphatic compounds, have been identified from extracts of the bark and leaves [33,34]. Likewise, extracts from the bark and leaves of I. laurina have shown the presence of terpenes such as phytol, the aliphatic nonacosane, and esterified aliphatic acids [35], whereas in a hexanic fraction obtained from the leaves of I. semialata, the main compounds isolated were triterpenes, such as lupeol, α-amyrin, oleanolic acid, and friedelin [30]. In this report, the presence of esterified aliphatic acids was identified and, as in other species of the Inga genus, the presence of lupeol has been established. However, the following compounds: hentriacontane, α-tocopherol, lup-20 (29)-en-3-one and 24-methylenecycloartan-3-one, are reported for the first time for this genus.

Anti-Inflammatory Activity of Organic Extracts from Inga jinicuil
The results corresponding to the anti-inflammatory study of the organic extracts are presented in Figure 3. At the same dose of 1.0 mg/ear, all of the extracts showed antiinflammatory activity. For the bark extracts, the percentages of inhibition were: Ij-BH 34.6 ± 3.0%, Ij-BD 67.3 ± 2.0%, and Ij-BHac 24.4 ±1.0%, and for leaf extracts, the corresponding percentages were: Ij-LH 34.9 ± 1.3%, Ij-LD 23.0 ± 1.0%, and Ij-LHac 49.6 ± 1.0%. For indo-  It is worth noting that this is the first report of a CG-MS analysis of hexanic extracts from Inga jinicuil. However, similar studies have been documented for other species of the Inga genus; such is the case for I. edulis, where triterpene compounds including lupeol and stigmasterol, as well as aliphatic compounds, have been identified from extracts of the bark and leaves [33,34]. Likewise, extracts from the bark and leaves of I. laurina have shown the presence of terpenes such as phytol, the aliphatic nonacosane, and esterified aliphatic acids [35], whereas in a hexanic fraction obtained from the leaves of I. semialata, the main compounds isolated were triterpenes, such as lupeol, α-amyrin, oleanolic acid, and friedelin [30]. In this report, the presence of esterified aliphatic acids was identified and, as in other species of the Inga genus, the presence of lupeol has been established. However, the following compounds: hentriacontane, α-tocopherol, lup-20 (29)-en-3-one and 24-methylenecycloartan-3-one, are reported for the first time for this genus.

Anti-Inflammatory Activity of Organic Extracts from Inga jinicuil
The results corresponding to the anti-inflammatory study of the organic extracts are presented in Figure 3. At the same dose of 1.0 mg/ear, all of the extracts showed antiinflammatory activity. For the bark extracts, the percentages of inhibition were: Ij-BH 34.6 ± 3.0%, Ij-BD 67.3 ± 2.0%, and Ij-BHac 24.4 ±1.0%, and for leaf extracts, the corresponding  It is worth noting that this is the first report of a CG-MS analysis of hexanic extracts from Inga jinicuil. However, similar studies have been documented for other species of the Inga genus; such is the case for I. edulis, where triterpene compounds including lupeol and stigmasterol, as well as aliphatic compounds, have been identified from extracts of the bark and leaves [33,34]. Likewise, extracts from the bark and leaves of I. laurina have shown the presence of terpenes such as phytol, the aliphatic nonacosane, and esterified aliphatic acids [35], whereas in a hexanic fraction obtained from the leaves of I. semialata, the main compounds isolated were triterpenes, such as lupeol, α-amyrin, oleanolic acid, and friedelin [30]. In this report, the presence of esterified aliphatic acids was identified and, as in other species of the Inga genus, the presence of lupeol has been established. However, the following compounds: hentriacontane, α-tocopherol, lup-20 (29)-en-3-one and 24-methylenecycloartan-3-one, are reported for the first time for this genus.

Anti-Inflammatory Activity of Organic Extracts from Inga jinicuil
The results corresponding to the anti-inflammatory study of the organic extracts are presented in Figure 3. At the same dose of 1.0 mg/ear, all of the extracts showed antiinflammatory activity. For the bark extracts, the percentages of inhibition were: Ij-BH 34.6 ± 3.0%, Ij-BD 67.3 ± 2.0%, and Ij-BHac 24.4 ±1.0%, and for leaf extracts, the corresponding  It is worth noting that this is the first report of a CG-MS analysis of hexanic extracts from Inga jinicuil. However, similar studies have been documented for other species of the Inga genus; such is the case for I. edulis, where triterpene compounds including lupeol and stigmasterol, as well as aliphatic compounds, have been identified from extracts of the bark and leaves [33,34]. Likewise, extracts from the bark and leaves of I. laurina have shown the presence of terpenes such as phytol, the aliphatic nonacosane, and esterified aliphatic acids [35], whereas in a hexanic fraction obtained from the leaves of I. semialata, the main compounds isolated were triterpenes, such as lupeol, α-amyrin, oleanolic acid, and friedelin [30]. In this report, the presence of esterified aliphatic acids was identified and, as in other species of the Inga genus, the presence of lupeol has been established. However, the following compounds: hentriacontane, α-tocopherol, lup-20 (29)-en-3-one and 24-methylenecycloartan-3-one, are reported for the first time for this genus.

Anti-Inflammatory Activity of Organic Extracts from Inga jinicuil
The results corresponding to the anti-inflammatory study of the organic extracts are   It is worth noting that this is the first report of a CG-MS analysis of hexanic extracts from Inga jinicuil. However, similar studies have been documented for other species of the Inga genus; such is the case for I. edulis, where triterpene compounds including lupeol and stigmasterol, as well as aliphatic compounds, have been identified from extracts of the bark and leaves [33,34]. Likewise, extracts from the bark and leaves of I. laurina have shown the presence of terpenes such as phytol, the aliphatic nonacosane, and esterified aliphatic acids [35], whereas in a hexanic fraction obtained from the leaves of I. semialata, the main compounds isolated were triterpenes, such as lupeol, α-amyrin, oleanolic acid, and friedelin [30]. In this report, the presence of esterified aliphatic acids was identified and, as in other species of the Inga genus, the presence of lupeol has been established. However, the following compounds: hentriacontane, α-tocopherol, lup-20 (29)-en-3-one and 24-methylenecycloartan-3-one, are reported for the first time for this genus.

Anti-Inflammatory Activity of Organic Extracts from Inga jinicuil
The results corresponding to the anti-inflammatory study of the organic extracts are presented in Figure 3. At the same dose of 1.0 mg/ear, all of the extracts showed antiinflammatory activity. For the bark extracts, the percentages of inhibition were: Ij-BH 34.6 ± 3.0%, Ij-BD 67.3 ± 2.0%, and Ij-BHac 24.4 ±1.0%, and for leaf extracts, the corresponding percentages were: Ij-LH 34.9 ± 1.3%, Ij-LD 23.0 ± 1.0%, and Ij-LHac 49.6 ± 1.0%. For indomethacin (Indo), which was employed as the reference drug, the inhibition percentage was 75.5 ± 2.2%. As can be seen, the two extracts with the greatest anti-inflammatory activity were Ij-BD followed by Ij-LHac, and the statistical comparison between the anti- It is worth noting that this is the first report of a CG-MS analysis of hexanic extracts from Inga jinicuil. However, similar studies have been documented for other species of the Inga genus; such is the case for I. edulis, where triterpene compounds including lupeol and stigmasterol, as well as aliphatic compounds, have been identified from extracts of the bark and leaves [33,34]. Likewise, extracts from the bark and leaves of I. laurina have shown the presence of terpenes such as phytol, the aliphatic nonacosane, and esterified aliphatic acids [35], whereas in a hexanic fraction obtained from the leaves of I. semialata, the main compounds isolated were triterpenes, such as lupeol, α-amyrin, oleanolic acid, and friedelin [30]. In this report, the presence of esterified aliphatic acids was identified and, as in other species of the Inga genus, the presence of lupeol has been established. However, the following compounds: hentriacontane, α-tocopherol, lup-20 (29)-en-3-one and 24-methylenecycloartan-3-one, are reported for the first time for this genus.

Anti-Inflammatory Activity of Organic Extracts from Inga jinicuil
The results corresponding to the anti-inflammatory study of the organic extracts are presented in Figure 3. At the same dose of 1.0 mg/ear, all of the extracts showed antiinflammatory activity. For the bark extracts, the percentages of inhibition were: Ij-BH 34.6 ± 3.0%, Ij-BD 67.3 ± 2.0%, and Ij-BHac 24.4 ±1.0%, and for leaf extracts, the corresponding percentages were: Ij-LH 34.9 ± 1.3%, Ij-LD 23.0 ± 1.0%, and Ij-LHac 49.6 ± 1.0%. For indomethacin (Indo), which was employed as the reference drug, the inhibition percentage was 75.5 ± 2.2%. As can be seen, the two extracts with the greatest anti-inflammatory activity were Ij-BD followed by Ij-LHac, and the statistical comparison between the anti- (12. It is worth noting that this is the first report of a CG-MS analysis of hexanic extracts from Inga jinicuil. However, similar studies have been documented for other species of the Inga genus; such is the case for I. edulis, where triterpene compounds including lupeol and stigmasterol, as well as aliphatic compounds, have been identified from extracts of the bark and leaves [33,34]. Likewise, extracts from the bark and leaves of I. laurina have shown the presence of terpenes such as phytol, the aliphatic nonacosane, and esterified aliphatic acids [35], whereas in a hexanic fraction obtained from the leaves of I. semialata, the main compounds isolated were triterpenes, such as lupeol, α-amyrin, oleanolic acid, and friedelin [30]. In this report, the presence of esterified aliphatic acids was identified and, as in other species of the Inga genus, the presence of lupeol has been established. However, the following compounds: hentriacontane, α-tocopherol, lup-20 (29)-en-3-one and 24-methylenecycloartan-3-one, are reported for the first time for this genus.

Anti-Inflammatory Activity of Organic Extracts from Inga jinicuil
The results corresponding to the anti-inflammatory study of the organic extracts are presented in Figure 3. At the same dose of 1.0 mg/ear, all of the extracts showed antiinflammatory activity. For the bark extracts, the percentages of inhibition were: Ij-BH 34.6 ± 3.0%, Ij-BD 67.3 ± 2.0%, and Ij-BHac 24.4 ±1.0%, and for leaf extracts, the corresponding percentages were: Ij-LH 34.9 ± 1.3%, Ij-LD 23.0 ± 1.0%, and Ij-LHac 49.6 ± 1.0%. For indomethacin (Indo), which was employed as the reference drug, the inhibition percentage was 75.5 ± 2.2%. As can be seen, the two extracts with the greatest anti-inflammatory activity were Ij-BD followed by Ij-LHac, and the statistical comparison between the anti-  It is worth noting that this is the first report of a CG-MS analysis of hexanic extracts from Inga jinicuil. However, similar studies have been documented for other species of the Inga genus; such is the case for I. edulis, where triterpene compounds including lupeol and stigmasterol, as well as aliphatic compounds, have been identified from extracts of the bark and leaves [33,34]. Likewise, extracts from the bark and leaves of I. laurina have shown the presence of terpenes such as phytol, the aliphatic nonacosane, and esterified aliphatic acids [35], whereas in a hexanic fraction obtained from the leaves of I. semialata, the main compounds isolated were triterpenes, such as lupeol, α-amyrin, oleanolic acid, and friedelin [30]. In this report, the presence of esterified aliphatic acids was identified and, as in other species of the Inga genus, the presence of lupeol has been established. However, the following compounds: hentriacontane, α-tocopherol, lup-20 (29)-en-3-one and 24-methylenecycloartan-3-one, are reported for the first time for this genus.

Anti-Inflammatory Activity of Organic Extracts from Inga jinicuil
The results corresponding to the anti-inflammatory study of the organic extracts are presented in Figure 3. At the same dose of 1.0 mg/ear, all of the extracts showed antiinflammatory activity. For the bark extracts, the percentages of inhibition were: Ij-BH 34.6 ± 3.0%, Ij-BD 67.3 ± 2.0%, and Ij-BHac 24.4 ±1.0%, and for leaf extracts, the corresponding percentages were: Ij-LH 34.9 ± 1.3%, Ij-LD 23.0 ± 1.0%, and Ij-LHac 49.6 ± 1.0%. For indomethacin (Indo), which was employed as the reference drug, the inhibition percentage was 75.5 ± 2.2%. As can be seen, the two extracts with the greatest anti-inflammatory activity were Ij-BD followed by Ij-LHac, and the statistical comparison between the anti-  It is worth noting that this is the first report of a CG-MS analysis of hexanic extracts from Inga jinicuil. However, similar studies have been documented for other species of the Inga genus; such is the case for I. edulis, where triterpene compounds including lupeol and stigmasterol, as well as aliphatic compounds, have been identified from extracts of the bark and leaves [33,34]. Likewise, extracts from the bark and leaves of I. laurina have shown the presence of terpenes such as phytol, the aliphatic nonacosane, and esterified aliphatic acids [35], whereas in a hexanic fraction obtained from the leaves of I. semialata, the main compounds isolated were triterpenes, such as lupeol, α-amyrin, oleanolic acid, and friedelin [30]. In this report, the presence of esterified aliphatic acids was identified and, as in other species of the Inga genus, the presence of lupeol has been established. However, the following compounds: hentriacontane, α-tocopherol, lup-20 (29)-en-3-one and 24-methylenecycloartan-3-one, are reported for the first time for this genus.

Anti-Inflammatory Activity of Organic Extracts from Inga jinicuil
The results corresponding to the anti-inflammatory study of the organic extracts are presented in Figure 3. At the same dose of 1.0 mg/ear, all of the extracts showed antiinflammatory activity. For the bark extracts, the percentages of inhibition were: Ij-BH 34.6 ± 3.0%, Ij-BD 67.3 ± 2.0%, and Ij-BHac 24.4 ±1.0%, and for leaf extracts, the corresponding percentages were: Ij-LH 34.9 ± 1.3%, Ij-LD 23.0 ± 1.0%, and Ij-LHac 49.6 ± 1.0%. For indomethacin (Indo), which was employed as the reference drug, the inhibition percentage was 75.5 ± 2.2%. As can be seen, the two extracts with the greatest anti-inflammatory activity were Ij-BD followed by Ij-LHac, and the statistical comparison between the anti- (40.49) α-Tocopherol 38 36 It is worth noting that this is the first report of a CG-MS analysis of hexanic extracts from Inga jinicuil. However, similar studies have been documented for other species of the Inga genus; such is the case for I. edulis, where triterpene compounds including lupeol and stigmasterol, as well as aliphatic compounds, have been identified from extracts of the bark and leaves [33,34]. Likewise, extracts from the bark and leaves of I. laurina have shown the presence of terpenes such as phytol, the aliphatic nonacosane, and esterified aliphatic acids [35], whereas in a hexanic fraction obtained from the leaves of I. semialata, the main compounds isolated were triterpenes, such as lupeol, α-amyrin, oleanolic acid, and friedelin [30]. In this report, the presence of esterified aliphatic acids was identified (26.74) Lup-20 (29) It is worth noting that this is the first report of a CG-MS analysis of hexanic extracts from Inga jinicuil. However, similar studies have been documented for other species of the Inga genus; such is the case for I. edulis, where triterpene compounds including lupeol and stigmasterol, as well as aliphatic compounds, have been identified from extracts of the bark and leaves [33,34]. Likewise, extracts from the bark and leaves of I. laurina have shown the presence of terpenes such as phytol, the aliphatic nonacosane, and esterified aliphatic acids [35], whereas in a hexanic fraction obtained from the leaves of I. semialata, the main compounds isolated were triterpenes, such as lupeol, α-amyrin, oleanolic acid, ( It is worth noting that this is the first report of a CG-MS analysis of hexanic extracts from Inga jinicuil. However, similar studies have been documented for other species of the Inga genus; such is the case for I. edulis, where triterpene compounds including lupeol and stigmasterol, as well as aliphatic compounds, have been identified from extracts of the bark and leaves [33,34]. Likewise, extracts from the bark and leaves of I. laurina have shown the presence of terpenes such as phytol, the aliphatic nonacosane, and esterified aliphatic acids [35], whereas in a hexanic fraction obtained from the leaves of I. semialata, (38. It is worth noting that this is the first report of a CG-MS analysis of hexanic extracts from Inga jinicuil. However, similar studies have been documented for other species of the Inga genus; such is the case for I. edulis, where triterpene compounds including lupeol and stigmasterol, as well as aliphatic compounds, have been identified from extracts of the bark and leaves [33,34]. Likewise, extracts from the bark and leaves of I. laurina have shown the presence of terpenes such as phytol, the aliphatic nonacosane, and esterified aliphatic acids [35], whereas in a hexanic fraction obtained from the leaves of I. semialata, (2.27) Stigmast-4-en-3-one 42 41 It is worth noting that this is the first report of a CG-MS analysis of hexanic extracts from Inga jinicuil. However, similar studies have been documented for other species of the Inga genus; such is the case for I. edulis, where triterpene compounds including lupeol and stigmasterol, as well as aliphatic compounds, have been identified from extracts of the bark and leaves [33,34]. Likewise, extracts from the bark and leaves of I. laurina have shown the presence of terpenes such as phytol, the aliphatic nonacosane, and esterified  It is worth noting that this is the first report of a CG-MS analysis of hexanic extracts from Inga jinicuil. However, similar studies have been documented for other species of the Inga genus; such is the case for I. edulis, where triterpene compounds including lupeol and stigmasterol, as well as aliphatic compounds, have been identified from extracts of the bark and leaves [33,34]. Likewise, extracts from the bark and leaves of I. laurina have shown the presence of terpenes such as phytol, the aliphatic nonacosane, and esterified aliphatic acids [35], whereas in a hexanic fraction obtained from the leaves of I. semialata, the main compounds isolated were triterpenes, such as lupeol, α-amyrin, oleanolic acid, and friedelin [30]. In this report, the presence of esterified aliphatic acids was identified and, as in other species of the Inga genus, the presence of lupeol has been established. However, the following compounds: hentriacontane, α-tocopherol, lup-20 (29)-en-3-one and 24-methylenecycloartan-3-one, are reported for the first time for this genus.

Anti-Inflammatory Activity of Organic Extracts from Inga jinicuil
The results corresponding to the anti-inflammatory study of the organic extracts are presented in Figure 3. At the same dose of 1.0 mg/ear, all of the extracts showed antiinflammatory activity. For the bark extracts, the percentages of inhibition were: Ij-BH 34.6 ± 3.0%, Ij-BD 67.3 ± 2.0%, and Ij-BHac 24.4 ±1.0%, and for leaf extracts, the corresponding percentages were: Ij-LH 34.9 ± 1.3%, Ij-LD 23 It is worth noting that this is the first report of a CG-MS analysis of hexanic extracts from Inga jinicuil. However, similar studies have been documented for other species of the Inga genus; such is the case for I. edulis, where triterpene compounds including lupeol and stigmasterol, as well as aliphatic compounds, have been identified from extracts of the bark and leaves [33,34]. Likewise, extracts from the bark and leaves of I. laurina have Even when species such as I. laurina, I. edulis, I. marginata, and I. jinicuil are employed to treat stomach and inflammatory disorders in traditional medicine, few studies have been conducted to confirm their attributed pharmacological properties. However, recent reports have shown the presence of flavonoids and other phenolic compounds in several of these species that may be associated with pharmacological effects [36]. The present study represents a preliminary approach in the assessment of the anti-inflammatory activity of I. jinicuil, with the bark extracts exerting a more consistent effect and Ij-BD showing the highest activity. It is noteworthy to mention that the chemical profile of this extract showed the presence of salicylates, terpenoids, and derivatives of epigallocatechin gallate, as well as derivatives of protocatechuic and coumaric acids, which may be associated with its biological effect [24,28,31]. In the case of the extracts from leaves, Ij-LHac showed the best inhibitory effect, and the analysis of its metabolic content revealed the presence of polyphenolic compounds, terpenoids, coumarins, vanillic acid derivatives, and flavonoidtype compounds such as apigenin derivatives, all of which have reported anti-inflammatory effects [19,34,37]. Furthermore, previous reports regarding several of the metabolites present in both extracts have postulated an anti-inflammatory activity that proceeds via the inhibition of cyclooxygenase enzymes (COX) [38][39][40], which happens to be the known mechanism of the reference drug (Indo) [40]. Finally, it is important to mention that the two extracts with the highest activity have the presence of terpenes and salicylates in common; these compounds are recognized for their analgesic and anti-inflammatory effects [24,31]. ences (p < 0.05). No extract reached an effect equal to or greater than that of Indo (Indomethacin). However, the comparison using the Tukey test of the effect of the extracts and the reference drug showed that there were no significant differences (p < 0.05) between some of the extracts, such as Ij-BH compared to Ij-LH and Ij-BHac compared to Ij-LD. Even when species such as I. laurina, I. edulis, I. marginata, and I. jinicuil are employed to treat stomach and inflammatory disorders in traditional medicine, few studies have been conducted to confirm their attributed pharmacological properties. However, recent reports have shown the presence of flavonoids and other phenolic compounds in several of these species that may be associated with pharmacological effects [36]. The present study represents a preliminary approach in the assessment of the anti-inflammatory activity of I. jinicuil, with the bark extracts exerting a more consistent effect and Ij-BD showing the highest activity. It is noteworthy to mention that the chemical profile of this extract showed the presence of salicylates, terpenoids, and derivatives of epigallocatechin gallate, as well as derivatives of protocatechuic and coumaric acids, which may be associated with its biological effect [24,28,31]. In the case of the extracts from leaves, Ij-LHac showed the best inhibitory effect, and the analysis of its metabolic content revealed the presence of polyphenolic compounds, terpenoids, coumarins, vanillic acid derivatives, and flavonoidtype compounds such as apigenin derivatives, all of which have reported anti-inflammatory effects [19,34,37]. Furthermore, previous reports regarding several of the metabolites present in both extracts have postulated an anti-inflammatory activity that proceeds via
The results obtained are interesting considering that in 2017 the WHO published a list of "priority pathogens" resistant to antibiotics, which include Pseudomonas aeruginosa (resistant to carbapenems) and Staphylococcus aureus (resistant to methicillin), emphasizing the urgent need for the search for new agents against these micro-organisms [5].
The antibacterial activity of bark and leaf extracts against Pseudomona aeruginosa (Pa) and methicillin-resistant Staphylococcus aureus (Sa1) can be attributed to the presence of several secondary metabolites: hentriacontane and α-tocopherol in Ij-BH, and polyphenols, flavonoids, and terpenoids in both Ij-BD and Ij-BHac [41]. Special attention may be paid to the presence of gallate and coumarin derivatives, since their antibacterial mechanism has been described at the cell membrane level by repressing the transport system of proteins and inhibiting the biofilm formation in clinical strains of Sa1 [17,42,43]. Furthermore, coumarin derivatives are considered as potential antibacterial agents that act as inhibitors to several binding proteins of Sa1 and potential competitive inhibitors of the DNA-gyrase [44,45]. It is worth noting that the chemical moiety responsible for the antibacterial activity of coumarins is the basic structure of benzopyrone, which resembles the structure of benzopyridone graph coupled to a simple quadrupole mass spectrometry detector, model 5972N (Agilent Technology, Santa Clara, CA, USA).
Volatile compounds were separated on an HP 5MS capillary column (25 m long, 0.2 mm i.d., with 0.3-µm film thickness). Oven temperature was set at 40 • C for 2 min, then programmed at 40-260 • C for 10 • C/min, and maintained for 20 min at 260 • C. Mass detector conditions were as follows: interphase temperature, 200 • C, and mass acquisition range, 20-550. Injector and detector temperatures were set at 250 and 280 • C, respectively. Splitless injection mode was carried out with 1 µL of each fraction (3 mg/mL solution). The carrier gas was helium at a flow rate of 1 mL/min. The identification of volatiles was performed, comparing their mass spectra with those of the National Institute of Standards and Technology (NIST) 1.7 Library and comparing these with data from the literature [47].

Anti-Inflammatory Activity
Male ICR mice with a weight range of 25-30 g, from Envigo RMS, S.A. de C.V., were used throughout the experiments. These animals were maintained in the Bioterium of Centro de Investigación Biomédica Del Sur (CIBIS-IMSS) under a 12 h light-dark cycle and constant temperature (23-25 • C) with free access to food and water. The animals were treated under the Mexican federal regulations for care and use of laboratory animals, NOM-062-ZOO-1999 Guidelines [48], and international ethical guidelines for the care and use of experimental animals [49]; the number of animals (n = 5) and the intensity of the noxious stimuli utilized were the minimum necessary to demonstrate the consistent effects of the pharmacological treatments. The animal studies were approved by the Ethics Committee of the Mexican Social Security Institute (R-2020-1702-008).
Auricular inflammation was induced following the method previously described [50]. The dose evaluated for the extracts was 1.0 mg/ear. A control group received acetone as vehicle, and Indomethacin (Indo, Sigma-Aldrich, Toluca, Mexico) 1.0 mg/ear was utilized as an anti-inflammatory positive control. All treatments were dissolved in acetone and applied topically on both ears immediately after the solution of 12-O-tetradecanoylphorbol-13-acetate (TPA, Sigma-Aldrich, Toluca, México) as an inflammatory agent. Six hours after the administration of TPA, the animals were euthanized by cervical dislocation. Circular sections 6 mm in diameter were taken from both the treated (t) and nontreated (nt) ears, which were weighed to determine the inflammation. The percentage of inhibition was obtained employing the expression below: % Inhibition = [Dw control − Dw treated/Dw control] × [100] where Dw = wt − wnt; wt is the weight of the section of the treated ear; and wnt the weight of untreated ear section.

Antibacterial Activity
The extracts were evaluated against bacterial strains ATCC: methicillin-resistant For the trials, cultures with 24 h of incubation (37 • C) were used and about 3-4 colonies were taken of each strain and diluted in Müeller-Hinton broth (MHb; Bioxon, Toluca, Mexico). The inoculums were adjusted using the 0.5 MacFarland scale (1.5 × 10 8 UFC/mL). Subsequently, dilution with distilled water was performed to obtain 1 × 10 4 UFC/mL. The MIC of extracts was determined by the microtiter broth dilution method [51]. Briefly, the samples (50 mg/mL) were dissolved in a DMSO-water mixture (20:80), and the tested concentrations were 3.37, 6.75, 12.5, 25, 50, 100, and 200 µg/mL. The samples were added to sterile microplates of 96 wells, along with 200 µL of MHb and 2 µL of inoculum (1 × 10 4 UFC/mL). The viability controls used were: MHb + DMSO + inoculum and MHb+ inoculum; Gentamicin (C + , 100 µg/mL; Sigma Aldrich, Mexico) was employed as the reference antibiotic. The plates were incubated at 37 • C for 24 h, and after incubation the MIC was determined by adding 30 µL of a solution (0.05%) of 3-(4,5-dimethylthiazol-2-yl)-2,5, diphenyl tetrazolium bromide (MTT, Sigma-Aldrich, Hong Kong, China) in every well, of which purple development was observed if there was viability of bacteria and colorless if there was no feasibility. All assays were performed in triplicate.

Statistical Analysis
For the analysis of the anti-inflammatory activity, the data were expressed as the mean ± standard error of the mean (SEM), and statistical significance was determined using an analysis of variance (ANOVA) with a confidence level of 95% (* p ≤ 0.05), followed by the one-tailed Dunnet test compared to Indo and the Tukey test. All analyses was performed using IBM SPSS statistics ver. 23.0 statistical program (GraphPad Software, IBM, San Diego, CA, USA).

Conclusions
This report presents the biological activity of organic extracts obtained from the bark and leaves of I. jinicuil. The anti-inflammatory activity tests showed moderate to good effects, with the dichloromethane extract from bark showing the highest activity, followed by the hexanic extract from leaves. Based on the findings of anti-inflammatory activity, it is possible to propose the exploration of the potential antinociceptive effect of the tested extracts, using an appropriate pharmacological model. Likewise, it was found that the three extracts from the bark of this plant have excellent antibacterial activity (primarily against methicillin-resistant Staphylococcus aureus and Pseudomonas aeruginosa strains) and this leads us to consider the possibility of extending antibacterial activity tests to a greater number of microbiological strains of clinical interest. On the other hand, it should be mentioned that, to our knowledge, this is the first approach to the phytochemical profiling of bark and leaves of I. jinicuil, which is consistent with the chemotaxonomic profiles reported for other species of Inga and suggest the presence of polyphenolic compounds, flavonoids, triterpenes, and lipid prenols, as well as aliphatic and esterified aliphatic lipids; these natural products may be responsible for both bioactivities assessed in this work. These results allow predicting a wide potential for future studies aimed at the isolation and structural characterization of compounds that might serve as molecular templates with specific biological activities. Finally, it is important to highlight that these results systematically contribute to the use in traditional Mexican medicine of a highly important sociocultural and nutritional species such as Inga jinicuil Schltdl & Cham. ex G. Don.