Sustainable Biochar and/or Melatonin Improve Salinity Tolerance in Borage Plants by Modulating Osmotic Adjustment, Antioxidants, and Ion Homeostasis

Salinity is persistently a decisive feature confining agricultural sustainability and food security in arid and semi-arid regions. Biochar (Bi) has been advocated as a means of lessening climate changes by sequestering carbon, concurrently supplying energy and rising crop productivity under normal or stressful conditions. Melatonin (Mt) has been shown to mediate numerous biochemical pathways and play important roles in mitigating multi-stress factors. However, their integrated roles in mitigating salt toxicity remain largely inexpressible. A completely randomized design was conducted to realize the remediation potential of Bi and/or Mt in attenuation salinity injury on borage plants by evaluating its effects on growth, water status, osmotic adjustment, antioxidant capacity, ions, and finally the yield. Salinity stress significantly decreased the plant growth and attributed yield when compared with non-salinized control plants. The depression effect of salinity on borage productivity was associated with the reduction in photosynthetic pigment and ascorbic acid (AsA) concentrations, potassium (K+) percentage, K+-translocation, and potassium/sodium ratio as well as catalase (CAT) activity. Additionally, borage plants’ water status was disrupted by salinity through decreasing water content (WC), relative water content (RWC), and water retention capacity (WTC), as well as water potential (Ψw), osmotic potential (Ψs), and turgor potential (Ψp). Moreover, salinity stress evoked oxidative bursts via hyper-accumulation of hydrogen peroxide (H2O2) and malondialdehyde (MDA), as well as protein carbonyl, which is associated with membrane dysfunction. The oxidative burst was connected with the hyper-accumulation of sodium (Na+) and chloride (Cl−) in plant tissues, coupled with osmolytes’ accumulation and accelerating plants’ osmotic adjustment (OA) capacity. The addition of Bi and/or Mt had a positive effect in mitigating salinity on borage plants by reducing Cl−, Na+, and Na+-translocation, and oxidative biomarkers as well as Ψw, Ψs, and Ψp. Moreover, Bi and/or Mt addition to salt-affected plants increased plant growth and yield by improving plant water status and OA capacity associated with the activation of antioxidant capacity and osmolytes accumulation as well as increased photosynthetic pigments, K+, and K+/Na+ ratio. Considering these observations, Bi and/or Mt can be used as a promising approach for enhancing the productivity of salt-affected borage plants due to their roles in sustaining water relations, rising solutes synthesis, progressing OA, improving redox homeostasis, and antioxidant aptitude.


Introduction
Borage (Borago officinalis L.; Boraginaceae), as a moderately salt-tolerant crop, represents an important medicinally cultivar that has about 20% γ-linolenic acid in the fixed indicate that Bi can induce encouraging impacts via enhancing the physio-biochemical and biological trials of saline soils, including enrichment minerals nutrients and recover soil bulk density, stabilization of soil structure, accelerating water-holding capacity, and high cation exchange capability and microbial activity [24,25]. Furthermore, Bi may have the capacity to decrease salt injury following three mechanisms [22,26]: (1) transient binding of sodium (Na + ) on its exchange position, and hence decreasing Na + uptake; (2) rising potassium (K + ) in soil solution, and so preserving Na + /K + ionic balance to lessen Na + uptake; and (3) rising soil moisture content, which may cause dilution effect and eventually causes a decline in Na + uptake. Finally, a small number of researchers have illustrated the effect of Bi on oxidative anxiety and antioxidant capacity in salt-affected plants [27].
Melatonin represents the earliest phylogenetic indole substance found in entire biota [9,13,28], at 0.1 pg g −1 (FW) to 20~30 µg g −1 (FW) [29]. Since 1995, it was initially detected in vascular plants, and the assimilation processes and metabolic occupations have been largely recognized [28,30]. This examination twisted the awareness for Mt as a potential efficient feature to motivate stress tolerance. The Mt function in plants within a stressful environment is not completely implicit, and it introduces an enthusiastic calculation, whether it performs as an antioxidant and/or plant promoter [12,28,31,32]. The advantageous function of Mt in stress lessening is generally attributable to elevated photosynthesis, enhancement of redox homeostasis, mitigation of oxidative burst, and regulation of the stress-responsive gene expression implicated in signal transduction [9,11,30]. Evidence shows that Mt and its metabolites acquire both hydrophilic and hydrophobic properties; thus, it is considered as a powerful antioxidant in plant response to stress factors [30,32]. It can simply pass through bio-membranes and dispense to any aqueous section, i.e., cytosol, mitochondria, etc. Additionally, Mt not only frankly deactivates ROS and reactive nitrogen species (RNS), but also motivates antioxidant enzymes, thus motivating its antioxidant capacity that organizes the rupture of H 2 O 2 in plants and defends them from oxidative anxiety [13,28,32,33]. Conversely, it is still indistinct whether such response of Mt against salinity is widespread for cultivars. Additionally, the mechanism of Mt-mediated salt tolerance is unclear. Consequently, it could be of enormous significance to exploit Mt as bio-activators for sustainable crop productivity without distressing the external environment.
Several types of research centered on the promotive effects of Mt and/or Bi on inducing salinity tolerance of several plants have been indicated previously. Whilst the individual encouraging impacts of Mt or Bi are well depicted, the synergistic impacts on salt-tolerance alleviation have hitherto to be elucidated. Consequently, the aims of the existing research were elucidating the regulatory approaches of Mt and Bi in mitigating salt-evoked injuries in terms of growth, yield, and some biochemical attributes that are modulated by salinity. We hypothesized that the individual and combined amendment of Mt and/or Bi possibly will lighten the oxidative injury by decreasing the Na + uptake in borage. The outcome of the current investigation will possibly emphasize the importance of using Mt and/or Bi as regulatory agents for getting the greatest production from the salt-affected region in existing worldwide warming predictions.

Organic Solutes and Water Relation
Organic solute was drastically amplified by either salinity or Bi amendment and/or Mt foliar application. The maximum value was recorded within Bi + Mt application under salinity that increased proline (133%) and soluble sugars (78%) relative to control plants. The data of leaf water potential (Ψw), osmotic potential (Ψs), turgor potential (Ψp), water content (WC), relative water content (RWC), water saturation deficit (WSD), and water retention capacity (WTC) established considerable variations in plant water status among control and salt stress in addition to Bi and/or Mt treatment (Table 1). Salinity considerably decreased the majority of plant water relation features, i.e., WC (22%), RWC (32%), Ψw (100%), Ψs (54%), Ψp (48%), and WTC (48%), and in the meantime enlarged WSD (133%) over control plants. The modifications stimulate OA status to be more positive for the water absorption. Application of Bi and/or Mt drastically improved RWC, WC, and WTC, whilst reducing WSD, Ψw, and Ψs, which induced OA aptitude and revival plant growth relative to unsprayed plants. The OA improved extremely with Bi and/or Mt application and may be predictable for maintaining Ψp of the leaf. Generally, the application of Bi+Mt treatment was more efficient than Bi or Mt application alone in rising leaf Ψp under a saline environment (Table 1). Table 1. The effect of melatonin (Mt) and/or biochar (Bi) supply on osmolyte accumulation, plant water relations, water potential (mega-pascal, Mpa), osmotic potential (Mpa), turgor potential (Mpa), and osmotic adjustment (Mpa) of borage plants grown in a saline medium (S, 5000 ppm NaCl). Values represent the means of five replicates, and the means not sharing the identical small letters in every column differ significantly from each other, consistent with the honest significant differences (HSD) test at a 5% probability altitude. Levels of significance are represented by *** p ≤ 0.001, and ns-not significant. (RWC, relative water content; WC, water content; WSD, water saturation deficit; WTC, water retention capacity).

Ion Accumulation and Translocation
The results presented in Table 2 indicated the alterations in K + , Na + , and Cl − ion accumulation, and the K + /Na + ratio as well as Na + and K + translocation (root to shoot) in the salt-stressed borage plants after Mt and/or Bi application. Under salinity, K + and the subsequent K + /Na + ratio were prominently (p < 0.05) reduced by 55% and 42% for K + and 92% and 72% for the K + /Na + ratio in either borage shoots and roots, respectively, above the non-salinized soil. Whereas Na + and Cl − were prominently increased in the shoots (799% and 399%) and in the roots (614% and 267%), respectively, when grown under salinity compared to control.
Nevertheless, Mt and/or Bi treatment remarkably enhanced K + (136% and 45%) and K + /Na + (390% and 218%) ratio in the shoot and root system accordingly, concerning the salinity devoid of Mt and/or Bi application. Moreover, Mt and/or Bi supply caused a considerable reduction in Na + and Cl − accretion in borage tissue compared to NaCl treatment ( Table 2). The outcomes concerning K + and Na + translocation in borage tissue exposed to salinity with or without Mt and/or Bi supplementation are shown in Table 2. Borage plants exposed to salinity demonstrated that K + translocation was reduced by 22% as compared to the control. Contrarily, salinity significantly (p < 0.05) enlarged Na + translocation by 25% relative to the control plants. The addition of Mt+Bi under salinity enhanced K + translocation (62%), while Na + translocation was consequently greatly reduced by 38% relative to the salinized borage plants lacking Mt and/or Bi supply (Table 2). Values represent the means of five replicates, and the means not sharing the identical small letters in every column differ significantly from each other, consistent with the honest significant differences (HSD) test at a 5% probability altitude. Levels of significance are represented by *** p ≤ 0.001, and ns-not significant.

Oxidative Biomarker
For deciding the function of Bi and/or Mt in the alleviation of salt-provoked oxidative damage, the oxidative biomarkers were estimated. In the current research, a substantial increase in H 2 O 2 concentration was noticeable in salt-affected borage pants. On the other hand, Bi and/or Mt treatment allowed for a comparatively lower accrual of H 2 O 2 in salt-stressed plants. The amendment of salt-stressed plants with Bi+Mt reduced H 2 O 2 accumulation by 50% relative to salt-stressed plants alone (Table 3). Malondialdehyde (MDA) can reflect the level of cellular injury. In this study, enhanced amounts of MDA (125%) were detected in salt-affected borage plants, relative to CK.
However, the level of MDA concentration was significantly lesser in plants with Mt and/or Bi applied to the salt-stressed borage plants relative to non-treated salt-affected plants ( Table 3). The most efficient in this regard was the soil supplementation with Bi associated with Mt spraying, which decreased MDA by 48% relative to the salt-affected plants (Table 3).
Proportionate to the control treatment, the protein carbonyl concentration under salinity increased drastically, by 72% (Table 3). When Bi and/or Mt were applied, the protein carbonyl concentration decreased. Application of Bi+Mt significantly reduced protein carbonyl concentration levels by 62%, corresponding to S plants, indicating that the Bi+Mt application had the inhibitor's impact on ROS production of the borage plant.
Salinity caused an upsurge in the leakage of cellular electrolytes from 59.46% to 57.52% in the third upper leaves in the main stem of the borage plant compared to the control plants. Conversely, the application of Bi and/or Mt along with salinity significantly leveled off salt damage on the membrane permeability (%) by reducing the rate of cellular electrolytes compared to salt-treated plants ( Table 3).

Antioxidant Enzymes
Under undesirable environments, plants utilize their antioxidant enzyme (i.e., SOD, POD, and CAT) to eradicate overload ROS for protecting plants from oxidative injury. The conducted study revealed a greater enhancement in the SOD (161%) and POD (16%) activity of salt-stressed plants than those of control plants. However, the extra addition of Bi and/or Mt in plant tissue of stressed borage plants further improved the activities of SOD and POD over salt-treated plants. The Bi+Mt had remarkably enhanced SOD and POD activity under salinity that was rose about 27% and 36%, respectively, compared to salt-affected plants (Table 4). Values represent the means of five replicates, and the means not sharing the identical small letters in every column differ significantly from each other, consistent with the honest significant differences (HSD) test at a 5% probability altitude. Levels of significance are represented by *** p ≤ 0.001, and ns-not significant.
Salinity stress significantly reduced CAT activity by 53% relative to the control. Application of Bi and/or Mt in saline circumstances improved CAT activity above untreated salt-affected plants (Table 4). Amongst them, the raise in CAT activity was mainly noticeable under Bi+Mt treatment. The CAT activity level was 93% superior, correspondingly than the salt-affected plants, demonstrating that Bi+Mt most noticeably stimulated the CAT activity of borage plants (Table 4). Table 4 confirms that NaCl significantly raised phenols (51%) and flavonoids (Flav, 33%); however, it appreciably reduced AsA (53%) concentration in the borage plant comparative to control. Superior phenols, AsA, and Flav were recorded in Bi-and/or Mt-treated plants within the saline circumstances. This maintains that Bi and/or Mt have an extraordinary antioxidant activity via eliciting an antioxidant accumulation. The maximum phenols and Flav concentrations were acquired through Bi+Mt treatment under salinity. Phenols (98%) and Flav (100%) were drastically amplified in contrast to untreated control plants. Alternatively, the supreme AsA concentration was proofed with Bi+Mt supplementation, which increased by 49% compared to salinized plants.

Photosynthetic Pigment
Salinity induces an extraordinary lessening in total chlorophyll (35%) and carotenoid (73%) concentrations above the control plants. Application of Bi and/or Mt to salt-exposed plants prevents the salt-related photosynthetic pigment shortage via preserving a superior concentration compared with salt-affected plants. The Bi+Mt application provides supreme values of photosynthetic pigments compared with saline conditions, which increased it by 43% and 239%, respectively (Table 5). Values represent the means of five replicates, and the means not sharing the identical small letters in every column differ significantly from each other, consistent with the honest significant differences (HSD) test at a 5% probability altitude. Levels of significance are represented by * p <0.05, ** p ≤ 0.01; *** p ≤ 0.001, and ns-not significant. (FW, fresh weight; DW, dry weight).

Growth Parameters
The morphological traits of borage under salinity conditions, with or without Mt and/or Bi supplementation, are given in Table 5. Salinity repressed the growth trials of borage plants corresponding to control plants. Plant height, shoot fresh weight, shoot dry weight, and leaf area were significantly declined (p < 0.05) by 47%, 58%, 52%, and 69%, respectively, by salinity stress relative to the control plants (Table 5). Corresponding to salt-affected plants alone, the application of Mt and Bi individually or in combination reduces the inhibition of borage plant growth under salinity. The greatest values of plant height, shoot fresh weight, shoot dry weight, and leaf area of salt-affected borage plants were obtained by soil supplementation with Bi plus Mt application, compared to the saline medium alone, which increased it by 80%, 115%, 85%, and 150%, respectively (Table 5).

Yield Attributed
Influence of salinity and Bi and/or Mt on borage plant yield, i.e., seed yield/plant, seed index, oil percentage, and oil yield/plant, are delineated in Table 6. It proves that salinity considerably (p < 0.05) decreased borage yield corresponding to the control. Salinity decreased seed yield/plant, seed index, oil percentage, and oil yield/plant by 46%, 50%, 29%, and 61%, respectively, in relation to the control plants. Conversely, Bi and/or Mt drastically raised yield features relative to non-treated plants. Bi and/or Mt application under salinity diminished the NaCl injuries on yield attributes greater than their relevant untreated salt-affected plants. Compared with salt-affected plants, the greatest yield attributes were recorded under Bi+Mt treatment, which increased seed yield/plant, seed index, oil percentage, and oil yield/plant by 58%, 81%, 37%, and 118%, respectively (Table 6) Table 6. The effect of melatonin (Mt) and/or biochar (Bi) supply on seed and oil yield of borage plants grown in a saline medium (S, 5000 ppm NaCl). Values represent the means of five replicates, and the means not sharing the identical small letters in every column differ significantly from each other, consistent with the honest significant differences (HSD) test at a 5% probability altitude. Levels of significance are represented by *** p ≤ 0.001, and ns-not significant.

Fixed Oil Constituents
The GC study of borage seeds' (Table 7)   Values represent the means of three replicates, and the means not sharing the identical small letters in every row differ significantly from each other, consistent with the honest significant differences (HSD) test at a 5% probability altitude. Levels of significance are represented by * p <0.05, ** p ≤ 0.01, and ns-not significant.

Discussion
Salinity represents the major abiotic hazard that restricts plant growth and biomass production [5,8,10,28]. The overall decline in salt-affected plant growth possibly will be attributed to the impacts of salt on several metabolic pathways, ultrastructure modification, and molecular responses [4,9,11,34] associated with the current results. Salt stress can disrupt plant ion homeostasis, which causes ion poisoning, disturbs membrane stability, disturbs photosynthetic activity, decreases the cell water and osmotic potentials, and stimulates osmotic stress as well as extraordinarily lessening plant dry weight [5,9]. Additionally, salinity induces the interruption of chloroplasts and lessens cell water potential, which then shortly evokes stomatal closure and smaller CO 2 incorporation, therefore withdrawing cell division [5,10]. Moreover, salinity evokes the accumulation of ROS, which accelerates oxidative injury and causes steady oxidative injury by the weakening of imperative cellular compounds, deactivates antioxidant capacity, interrupts plant-water relations, and lessens nutrient uptake [3,5,9,10,13].
Recently, Mt as an activator has attracted the attention of botanists [31,32,35]. For example, it provides biochemical and molecular resistance alongside numerous environmental stresses via its participation in indicating modifiable stress [9,13,28,32]. In the current investigation, Mt spraying improved plant growth of the borage plant, compatible with the outcomes of Ahmad et al. [13], who established that Mt lessens oxidative anxiety within a stress environment. Moreover, Mt decreased ethylene assembly throughout the regulation of the MaACO1 and MaACS1 expression [36]. Biochar (as organic fertilizer with activated carbon) amendment to saline soils is accounted for improving plant development. The encouraging impact of Bi on plant growth under regular or stressful conditions agrees with the results reported by Ibrahim et al. [27], Yang et al. [5], and El-Gamal et al. [37]. The direct growth improvement of Bi application was associated with increasing nutrients' supply, i.e., calcium (Ca 2+ ), magnesium (Mg), phosphorus (P), K + , and sulfur (S), etc., to the plant; however, the indirect method comprises enhancing soil physiochemical and biological features [5,22,23]. In addition, the impact of Bi stimulation is possibly accredited to a decrease in transpiration, preservation of a superior net photosynthetic rate (Pn), water use efficiency, RWC, the regulation of ribulose-bisphosphate carboxylase oxidase (RuBisco), and lastly, the amplification of the plant antioxidant capacity [5,27]. These obtained data recommended that the application of Mt and/or Bi drastically balances the attenuation in plants' growth, and improves the stress tolerance by serving as ROS scavengers, enhancers of the nutrient uptake, and promising motivators of plants' development under salinity.
Leaf yellowing is the key visible symbol of salinity injury in several plants [5,10,27]. The criticism of salinity on total chlorophyll and carotenoids are primarily coupled with a huge ROS assembly, which stimulates chloroplast clustering and destruction, as well as photosynthetic dysfunction [10,13]. The ROS hasten the structural alteration of pigmentprotein complexes following three stages [38]: (i) the withdrawal and weakening of the antenna complex protein positioned in the grana; (ii) the substitute of Mg 2+ with H + ions, which evokes the pheophytinization of chlorophylls; and (iii) damage in the grana of the chloroplast. In this regard, Farouk and Arafa [34] found that salinity stress raised chlorophyll decomposition, possibly as a result of the buildup of Na + , which possibly hastens chloroplast ultrastructure modification. Moreover, stressful conditions accelerate the reduction in the content of the intermediaries Proto IX, Mg-Proto IX, and Pchlide throughout the chlorophyll assimilation [16], and reduce the hlD, ChlH, and Chl I-1 gene expression-encoding subunits of Mg-chelatase [39].
Comparable studies indicated that Mt [28,31,33,35] and/or Bi [5,23,40] supplementation raised total chlorophyll and carotenoids under normal or stressful environments, leading to a marinating greenish of the leaves. The impact of Bi and/or Mt on total chlorophyll and carotenoids contents is possibly associated with accelerating antioxidant enzyme activities or built up antioxidant capacity (Table 4), as confirmed by Ahmad et al. [13] and Chrustek and Olszewska-Stonino [41]. Generally, Mt represses salt inhibition of the ferredoxin gene PetFin rice [42], whereas ferredoxin defends chlorophyll from deprivation [43]. Additionally, Mt and/or Bi supplementation provokes Mg 2+ , carotenoid, and flavonoid buildup, which is attractive for chlorophyll assimilation or to guard chlorophyll against ROS. Lately, Mt spraying stimulated a visible reduction in the pheophorbide oxidase gene expression, senescence-correlated gene 12 (SAG12), and chlorophyllase gene expression [44].
Salinity episodic ion homeostasis induced a nutritional imbalance, leading to a buildup of poisonous ions, i.e., Na + and Cl − , which is connected to the decreased K + and K + /Na + ratio values [3,8,9,28]. Under salinity, the foremost basis of declined K + uptake may be caused by the ion imbalance and the aggressive uptake of Na + and Cl − with definite ions, i.e., potassium [4]. Moreover, the higher buildup of Na + and Cl − competed with the absorption of certain ions like K + [12,28]. Additionally, extreme Na + does not merely obstruct K + absorption, but additionally upsets the cellular metabolic pathways, leading to nutritional confusions, restrictive absorption of vital ions, and eventually yield reduction [7].
Current studies have shown that Mt application improves plants' ion homeostasis under salinity by increasing K + and decreasing Na + , leading to a drastically higher K + /Na + ratio. That was confirmed by Ren et al. [9], Wei et al. [32], and Zhang et al. [28]. Enhanced ion homeostasis is possibly connected to the upregulation of numerous genes, like the NHX1-encoding gene (responsible for the excessive transfer of Na + into vacuolar; [45]), salt overly sensitive 1 (SOS1, responsible for conveying Na + outside the cells; [46]), and AKT1-encoding gene (responsible for absorption of K + from the soil and moving it into the roots; [47]). Thus, upregulating NHX1, SOS1, and AKT1 gene expression causes a rise in K + and decrease Na + in plant cells, thus enhancing the plant's stress tolerance. Accordingly, NHX1 and SOS1 expression was superior in Mt-sprayed rapeseed seedlings to the non-treated ones that were linked with the lesser Na + /K + ratio by decreasing Na + and increasing K + [48].
Bi supplementation significantly decreased Na + entry into the cells, therefore enriching plant cells with K + , resulting in an increasing K + /Na + ratio and improving plant growth under salinity [5,23]. Biochar has a high adsorption capacity, which possibly efficiently decreases Na + uptake by plants [22,25]. Further, Bi serves as a direct supply of some ions, i.e., K + , Ca 2+ , and Mg 2+ [22,25]. The elevated Ca 2+ and Mg 2+ liberated by Bi can move Na + on the exchange site, additionally allowing for higher Na + leakage and lesser soil salinity, thus improving soil properties [49].
In plants, a higher K + /Na + ratio is the main feature, which assists salt-tolerant genotypes to grow healthy under salinity and to defend metabolic pathways by enhancing protein assimilation, regulating enzyme activities, and maintaining cell turgor [50]. The function of Bi and/or Mt in raising nutrient uptake is not completely implicit. In harmony with the current investigation, Ren et al. [9] confirmed that ion content was amplified in stress-affected plant tissue once Bi or Mt supplementation was administered, respectively. This promoting influence on neutralizing the corruption's impacts of salinity on nutrient content may be associated with the enhancement in nutrient uptake, preserving membrane permeability, and/or probably enhancing root system progress [51].
Over-production of organic solutes (OS), i.e., proline and soluble sugars, in borage with salinity and Bi and/or Mt supplementation (Table 3) is an approach for protecting optimal cell Ψw and defensive nucleic acids and macromolecule structures [52]. According to the current outcomes and previous reports, salinity [3,9] or Bi supplementation [51] and Mt supplementation [9,13] elicited OS production in various plants, probably to supply superior protection alongside salt stress. Induction in OS accumulation has two possible physio-biochemical responses: (i) decreasing cell osmotic potential, and thus maintaining plant-water relations [16,17]; and (ii) stabilizing biomembranes and macromolecules by regulating salt stress-responsive gene expression [53]. Proline can work as an osmoprotectant, a protein preservative, and in control cell differentiation, which is vital for plant revival under stressful factors [54]. Proline may also perform as a prevailing ROS elimination and defend the cellular constituents alongside oxidative damage [12]. The higher accumulation of proline under salinity or Mt and/or Bi supplementation could be a result of the modification in the activities of proline assimilation, degrading enzymes, protein degradation, and/or gene (δ1-pyrroline-5-carboxylate synthetase, ProDH) expression [53]. Soluble sugars play a decisive role in the OA, turgidity, and bimolecular stability, and offer energy and carbon backbones, which assist cells to develop quickly and assimilate the requisite organic substances [55]. The superior production of soluble sugars under salinity or Mt and/or Bi application could be owed to increased RuBisco and carbonic anhydrase enzyme activities [53,56]. Incidentally, Mt spraying enhanced the production of soluble sugars by enhancing sucrose synthesis-related gene expression, which assists in preserving plants' cellular integrity [57].
The preservation of elevated water status under stress is a prevailing outcome in crop cultivars. The WC, RWC %, and WTC levels in Bi and/or Mt application plants under saline conditions were retained at levels near to the control, as confirmed by previous research [28,32,51,58]. In the current investigation, salinity drastically decreased WC and RWC, but Bi-and/or Mt-treated borage plants established higher WC and RWC levels than untreated plants (Table 1), which delegated that Bi and/or Mt application played an important role in maintaining superior water relations and enhanced the salinity tolerance of borage. It is still undecided how Bi and Mt are able to maintain water status in plants within stressful environments. The increase in water preservation with Bi and/or Mt in stress-exposed plants might be coupled with (i) stomatal closure, (ii) a decrease in transpiration rate, or (iii) enhanced adventitious root growth. It is well known that Bi can maintain water in itself and accordingly elevate the water accessible to plants within stress conditions [58]. Additionally, Bi application enhanced soil aggregate stability, decreased soil bulk density, increased soil surface area, and increased soil water-holding capacity, resulting in enhancing soil moisture retention efficiently [24]. Additionally, Mt spraying might avoid crop water defeat through increasing leaf cuticle thickness [59].
Maintaining cell turgor next to decreasing Ψs is the main protection approach of plants within stressful conditions. Salinity and Bi and/or Mt supplementation hastened OS production, which probably decreased cell Ψs. Decreasing Ψw successively helps in preserving cell Ψp via an energetic declining of Ψs [16,28]. The reduction in RWC and Ψw is a decisive indication of stress confusion, which affects the water uptake into meristematic tissues [60]. The current data are in agreement with the previously accessible outcomes on the Ψw, Ψp, and RWC, representing decreased values with increasing stress times [28]. Biochar and/or Mt addition motivated leaf OS production as a result of decreased plant Ψw under stress, which is a recognized stress adaption to avoid plant dehydration and permanent wilting, and therefore preserve the finest turgor and regulate water uptake during transpiration [61]. The elevated hydrophilic inner surface area of the Bi particles, with enduring and storage holes, improves the water-holding capacity, and therefore decreases the soil evaporation rate [62]. Commonly, Bi and/or Mt enhanced the ion uptake, principally K + , which assists in preserving the plant Ψs [28] attributed to its extra cation concentration, which regulates the water status of the plant under a stressful environment. Kammann et al. [63] also documented a lessening in the transpiration rate in Bi-supplemented borage plants, which can be a cause for the enhanced Ψp and RWC of Bi-supplemented borage plants.
Under salinity, plants build up numerous methods for surviving, i.e., escape, tolerance, and avoidance, including OA capacity [64]. The OA represents the most applicable approach for tolerating environmental disorders [65]. Better performance of borage under salinity due to Bi and/or Mt application might be due to sustaining OA ability throughout harmonization with superior energetic solute accumulation and preservation of Ψw and Ψp, which is necessary for maintaining a typical development [65]. Accordingly, the cell Ψs is lessened, which successively sequentially preserves water homeostasis and enhances the aptitude of the cell to protect Ψp; this is desirable for enhancing water preservation, cell expansion, stomatal occupation, CO 2 fixation, and stabilizing macromolecules, which induces the enhancement of photosynthesis competence and rising stress tolerance [65].
Mitochondria and chloroplasts' oxidative pathways generate ROS during the plant's life duration. Small ROS production illustrates the encouraging effect and acquires function as a signal through the cell-restoring process. Plant cells can preserve small ROS concentrations under normal circumstances, since the cell antioxidant capacity realizes equilibrium between the eradication and the assembly of ROS [66]. Correspondingly, under stressful conditions, these self-protective strategies are overdriven by the rapid temporary production of an enormous amount of ROS, identified as oxidative rupture [67]. As seen with earlier research, the exposure of borage plants to salinity results in an amplified buildup of H 2 O 2 , MDA, and protein carbonyl groups. It can be implicit that extreme ROS buildup potentially causes oxidative injury, which devastates the ROS mitigation system of plants, leading to membrane dysfunction through hyper-accumulation of MDA and protein oxidation [28,40,53].
Amongst ROS, extra awareness has been paid to H 2 O 2 , owing to its modest steadiness and capability to enter the cellular membrane as an unaffected substance in various plants. The existing data propose that oxi-reductive rupture is provoked by H 2 O 2 buildup, which accelerates the over-production of MDA and cell membrane dysfunction, contributing to growth reduction. These judgments confirmed existing outcomes in previous research [13,28,41]. A commonly documented damage of salinity in plants is membrane dysfunction, the majority of which is probably through ROS gathering.
Consistently, most of the studies indicated that Mt and/or Bi alleviate ROS levels elicited by stress factors [13,28,32,33,51]. Constantly, the existing exploration assumed that Bi and/or Mt supplementation commonly moderated ROS injuries and preserved membrane permeability percentage; nevertheless, the maximum decline in oxidative biomarkers' assembly was monitored in salt-affected plants that were treated with Bi+Mt. These outcomes suggest that Mt and/or Bi may mitigate salinity related to membrane damage by lessening H 2 O 2 assembly (Table 3). On the contrary, Bi and/or Mt supplementation displayed less oxidative injury by hampering the creation of H 2 O 2 and MDA (Table 3) [13,28,32,51]. However, this lessening is not only credited to enhance antioxidant enzymes, but can also be recognized as a non-enzymatic solute accumulation [11,33]. Even so, the function of Mt and/or Bi on the ROS neutralizing method was not precisely calculated. It can be confirmed that the decline in H 2 O 2 assembly by Bi and/or Mt application is possibly due to H 2 O 2 trapping via the extra-production of phenolics and the modulation of antioxidant enzyme activities (Table 5).
Melatonin performs as a universal antioxidant that interrelates with ROS and directly alleviates it, owing to its antioxidative efficiency and convenience [28,30,53]. Through the ROS-lessening cascade, a distinct Mt can scavenge about 10 ROS/RNS that vary from additional conservative antioxidants by upregulating the antioxidant enzymes' coding gene expression and activation of mitochondrial uncoupling proteins (UCPs) [68]. Melatonin directly mitigates H 2 O 2 and improves the antioxidant enzymes' ability to eliminate H 2 O 2 by upregulating the expression of Mt assimilation genes. This is possible since Mt, as an electron donor, directly reacts with ROS and their associated molecules, and Mt is itself oxidized to an indole cation free radical, which consecutively reacts with intracellular O 2 and then oxidizes to constant N-acetyl N-formyl-5-methoxykinamine (5-MAFK). Under salinity, Mt spraying also drastically improved CAT, SOD, POD, and glutathione reductase relative to non-treated plants [28,32,33]. Furthermore, Mt interacts with ROS by enhancing antioxidant solutes (AsA-GSH) [69]. These results propose that exogenous Mt may perhaps progress cellular redox homeostasis by activating antioxidants' capacity to nullify saltinduced ROS, consequently enhancing plant salt tolerance.
A little investigation into the impacts of Bi on oxidative anxiety and antioxidant enzyme activities in salt-affected plants was conducted. Accordingly, the supplementation of Bi drastically declined H 2 O 2 and MDA concentrations under salinity (Table 3), demonstrating that Bi application efficiently declined oxidative anxiety. Comparable mitigations by Bi in oxidative injury and antioxidant enzyme activities of salt-affected plants were documented by Ibrahim et al. [27]. Torabian et al. [70] established that Bi decreased the assembly of ROS and DPPH activity in leaf cells and amplified the chlorophyll index under salinity. Additionally, the reduction of Na + and Cl − toxicity has an impact through adding the Bi to the soil and reducing O 2• − and H 2 O 2 , which accelerates a decline in antioxidant activities. These outcomes advocated that Bi could enhance plant biomass under salinity by decreasing oxidative anxiety and activating antioxidant enzymes.
Earlier research recognized that, within stressful conditions, crops proficiently moderated oxidative anxiety and maintained redox homeostasis via a serious ROS-mitigating system [13,51]. Normally, SOD is a decisive constituent of the plant's antioxidant protection scheme that is utilized as the early stage of protection, which changes superoxide ions into H 2 O 2 and oxygen. The POD is a prime enzyme that induces the fast elimination of H 2 O 2 at its product sites with diverse organic and inorganic substances [71]. The boost in SOD and POD might have been a decisive reaction to the assembly of superoxide radicals by salt-provoked blocking of the mitochondria electron transport pathway [72]. Generally, CAT is the main common oxidoreductase responsible for breaking up H 2 O 2 into H 2 O and O 2 , with a tremendously elevated turnover number (one CAT molecule could covert 6 million H 2 O 2 molecules every second, thus combating salt-induced oxidative stress [67]). This reduction in CAT is certainly caused by the inhibitory effect of salt on the enzyme system itself, or probably through the activation of the enzyme-bound heme group.
The addition of Bi and/or Mt accelerates a speedy ROS eradication via improving SOD, POD, and CAT activities, which established that there was a proficient plant ROS-mitigating capacity (Table 4). This was confirmed in the experiment with borage, in that Mt and/or Bi supplementation scavenges H 2 O 2 and induces the activation of the plant antioxidant system within salinity conditions [11,13,27,32,33]. Melatonin has an efficient antioxidant aptitude and enhances the activity of the antioxidant enzyme, therefore decreasing peroxidative injury [13,28,30,32]. This may have been attributable to upregulating the expression of genes (APX1/2, CAT1, and FSD1) transcripts associated with antioxidant enzymes and decreasing the deprivation of energetic substances to progress the activity of antioxidant enzymes and the expression of antioxidant genes [73]. Additionally, it controlled genes occupied in ascorbate assimilation, i.e., VTC4 and APX4, within salinity conditions. This may clarify the effect of Mt in improving the plant antioxidant aptitude [32]. Consequently, Bi and/or Mt application is able to reduce the salt-mediated destructive effect of the ROS scavenging system of borage plants and can enhance the resistance in plants under stress conditions. Diverse non-enzymatic antioxidants occupy an adjacent function in plant protection mechanisms including AsA, soluble phenolic compounds, and Flav, which perform as ROS mitigators in coincidence with antioxidant enzymes or independently. Antioxidant substances are engaged in different metabolic pathways, and they not only have a crucial role in plant tolerance, but also proceed as enzyme cofactors, and additionally affect plant development [74]. The recent research assumed that the concentrations of AsA, phenols, and Flav were strictly decreased or increased, respectively, in the borage plants under stress. These remarks documented that NaCl aggravated oxidative injury in borage by deteriorating the antioxidant aptitude. In contrast, the supplementation of Bi and/or Mt improved the antioxidant control of borage by raising the level of AsA, phenols, and Flav, which led to decreasing the oxidative burden from improved salt tolerance.
Ascorbic acid is a chief plant antioxidant that occupies the main function in withdrawing ROS as a result of its ability to provide electrons in a wide range of enzymatic and non-enzymatic reactions. It is able to lessen H 2 O 2 to H 2 O and O 2 through the APX reaction [75]. Currently, the AsA concentrations of borage plants decreased significantly under salt stress. The decline in AsA level under salinity designated that a little AsA was oxidized to dehydroascorbic acid (DHA). After the application of Bi and/or Mt, the AsA concentration of every treatment was considerably enlarged ( Table 4). The successful increase in AsA concentration by Bi and/or Mt is a tremendous sign of a decline in salinityinduced ROS assembly in salt-affected borage plants and lessens oxidative injury provoked by salinity [28]. This may be accelerated by Mt raising monoascorbate reductase (MDHAR), DHAR, and GR activity and hastening the restoration of AsA, thus ensuring moderately higher levels of AsA in borage plants within salinity and improving resistance to salinity.
Phenolic substances correlated straight with antioxidant success in plants, as they have electron-donating intermediaries, and consequently diminish extra ROS constructions [76]. Regularly, various outcomes that are similar to the assembly of phenol in plant tolerance are augmented within salinity [4,28], Mt [28], or Bi [37]. This accretion was probably provoked by activating the phenyl-propanoid pathways, thus enhancing the expression of the phenyl-aminolyase gene [77].
Flavonoids are broadly planted secondary products that are linked to their interface with the environment, including the ROS modulation status. Numerous investigations showed a boost in Flav in salt-stressed plants treated with or without being treated by Mt [28,78]. The biological distinctiveness of Flav is coupled with their prospective cytotoxicity and ROS eliminators. Flavonoids represent prevailing antioxidants achieved in the chelation of transition metals (Fe 2+ ), which restrict ROS formation via the Fenton reaction [79].
The annual global financial beating induced by salinity is 27.5 billion USD [7]. The decline in crop yield under salinity was indicated earlier by Farouk and Arafa [34], Sofy et al. [4], and Yang et al. [5] and confirms the observations of the present study. For several salt-affected plant cultivars, the reduction in yield is regularly coupled with lesser photosynthesis levels [5,10]. There are two achievable causes for the salt-accelerated photosynthesis reduction: stomatal closure and chloroplast modification [5]. The parameters of chlorophyll fluorescence consist of maximum photochemical efficiency of PSII (Fv/Fm), photochemical quenching (qP), non-photochemical quenching [Y(NPQ)], and definite photochemical effectiveness of PSII [Y(II)], etc. [10]. Additionally, salinity stress decreased pollen viability and stigmatic receptivity [80].
The current study successfully established that Mt stimulated salt tolerance; which was validated by Yang et al. [5]. The encouraging impact of Mt on yield in diverse crops may be caused by their impacts on the rising carbonic anhydrase activity and accelerating RuBisco, which stimulated CO 2 assembly and photosynthetic aptitude, which is reproduced by exploiting plant biomass production [28]. Furthermore, Mt acts to enhance vital functions in plant reproduction, i.e., floral differentiation, fertilization, and seed development, improves pollen -stigma interaction and improves stigmatic exudation and accelerates pollen tube development, which is desired for fertilization success [81,82].
Biochar applications are known to increase agricultural productivity [23,58], although the precise mechanistic influences of Bi have not been identified. Huang et al. [83] demonstrated that the rice yield was enlarged by 8%-10% after Bi application. There are numerous causes to explain why Bi may be able to boost the crop yield. Biochar supplementation stimulated leaf photosynthetic performance and diminished oxidative anxiety, leading to elevated plant biomass and yield under a saline environment [5]. Biochar is recognized to alter soil physiochemical characteristics and quality [25,84], thus distressing root biomass, optimizing root morpho-biochemical attributes, and subsequently boosting crop productivity [85]. Raising the soil pH can influence the ionic charge equilibrium within and outside the root [24], and this modification in the cation/anion ratio might contribute to pH alteration in the plant xylem sap [86]. Improved xylem sap pH may cause a considerable strengthening of ABA signaling causing stomatal closure in plants under salinity [87].
The oil was rich in polyunsaturated fatty acids, chiefly oleic and linoleic and linolenic acids, which have therapeutic importance and, additionally, the oil contained a little quantity of C18:3 linolenic-ω3 that may cause a superior oil stability to marketable oils [88]. For example, soyabean oil has up to 6.8% of linolenic-ω3, and it is less steady because of rapid oxidation compared to Salicornia bigelovii seed oil, which has merely 1.4% of this fatty acid [88]. The occurrence of saturated acids (17.17%, 16.67%, 16.03%) in borage seed oil in control, salt, and Bi+Mt under salinity stress, respectively, represent a chief quantitative variation against marketable oils. Intermediary principles of saturated acids were established in seeds from Salicornia brachiata (16.5%) [89] and the chenopod Suaeda fruticosa (17.0%) [90]. similarly elevated levels of palmitic acid (21.8%-29.4%) were established in seeds of salt fat and coastal dune halophytes (Arthrocnemum macrostachyum, Haloxylon stocksii, Alhagi maurorum, Cressa cretica, and Halopyrum mucronatum) from Asia [90]. The pots were set in a completely randomized design, which involved five treatments with five replicates: (1) non-saline soil devoid of NaCl or Bi or Mt, (2) saline soil with 5000 mg NaCl/kg soil, (3) saline soil with Bi at 5% air-dried soil, (4) saline soil with Mt spraying (100 µM), and (5) saline soil with Bi and Mt. The preferred salinity level was derived from a pilot testing, which utilized 1250, 2500, 5000, and 7500 mg NaCl/kg soil, for 4 days, and showed that plant wilting occurred under 7500 mg NaCl/kg soil; however, there was no noticeable wilting in plants under 1250, 2500, and 5000 mg NaCl/kg soil. Salinized soil was prepared by pouring the proper quantity of NaCl as designated before in every pot via irrigation water. Additionally, the appropriate Mt or Bi levels were selected based on our former investigation and further references [37,38,48]. Bi was added throughout preparing the pots at the desired concentration, whereas Mt foliar application (200 mL pot −1 ) was done thrice at 40, 50, and 60 days from cultivation, until dripping using a hand sprayer, whilst concurrently covering the pot surface to avoid the Mt solution from coming in reaching to the soil surface.

Sampling Date
The plants from all treatments were harvested at 90 days from sowing for evaluating morphological and biochemical attributes. All determinations were performed using five replicates except oil constituents, which were performed on three replicates only.

Morphological Characteristics
The plants were collected and we measured the plant height and shoot fresh weight; after that, samples were oven-dried at 70 • C for shoot dry weight assessment. In addition, the leaf area was measured according to the method of Koller [92].

Total Chlorophyll and Carotenoid Concentration
Total chlorophyll and carotenoid concentrations (mg g −1 FW) within the fifth upper leaf were extracted with ice-cold methanol supplemented with sodium carbonate and quantified spectrophotometrically (T60 UV-Visible spectrophotometer, PG Instrument Limits, UK), at 470, 653, and 666 nm following the Lichtenthaler and Welburn [93] method.

Ion Percentage
Sodium (Na + ) and potassium (K + ) were extracted by the Motsara and Roy [91] protocol and assessed by a flame photometer. On the other hand, chloride (Cl − , mg g −1 DW) was determined by 0.01 N of silver nitrate solution with 5% potassium dichromate as an indicator [94]. Additionally, K + and Na + translocation (from root to shoot) was designed following Malik et al.'s [95] equation.
Translocation factor = Ionic concentration in leaves ionic concentration in roots (1)

Organic Solutes and Water Relations
Proline (mg g −1 FW) was estimated by a ninhydrin colorimetric scheme [96]. The shoot sample was homogenized in aqueous sulpho-salicylic acid (3%) and centrifugation. Therefore, the 2 mL reaction mixture was reacted with 2 mL of glacial acetic acid and 2 mL of acid ninhydrin in a test tube for 30 min at 100 • C in a water bath, and finally, toluene was added. The toluene part absorbance was read at 520 nm. Meanwhile, total soluble sugar concentrations (mg/g DW) were determined based on the anthrone colorimetric technique recorded by Sadasivam and Manickam [97].
Plant water relation attributes were quantified in the shoot tips. Shoot tips were weighted for achieving fresh weight (FW) and then floated in distilled water in a closed Petri dish for 8 h, to measure the turgid weight (TW). Finally, the plant samples were consequently dehydrated at 80 • C in a pre-heated oven for the dry weight (DW) assessment [98]. Relative water content (RWC) and water content (WC) were determined by the following equations.
Leaf water potential (Ψw) was judged next to the Chardakoves technique as explained by Taiz and Zeiger [100]. We defined the osmotic potential (Ψs) following the Van't Hoff equation, Ψs (MPa) = −miRT, where m is the molality (moles per 1000 g), i is the ionization constant, R is the gas constant, and T is the temperature (k). Turgor potential (Ψp) was calculated as the differences between Ψw and Ψs [101]. Alternatively, osmotic adjustment (OA) was calculated as the difference in Ψ0 between the treated plants and the control ones [102].

Oxidative Biomarkers
Hydrogen peroxide (µM/g FW) was determined as per the Tariq et al.'s [103] protocol, by homogenizing the fresh plant tissue in chilled acetone, followed by centrifugation. The supernatant was treated with 0.1% titanium reagent and then with ammonium solution (25%); afterwards, centrifugation was done, and the yellow mixture optical density (OD) was read at 415 nm. The concentration of H 2 O 2 was designed via a pure H 2 O 2 standard curve, involving the extinction coefficient of H 2 O 2 (µM −1 cm −1 ).
Lipid peroxidation (nM MDA/g FW) was estimated by assessing malondialdehyde (MDA) production via the thiobarbituric acid (TBA) technique [104]. The MDA was extracted from 0.5 g leaf FW, with 0.1% trichloroacetic acid (TCA). The homogenate was centrifuged for 15 min at 10,000× g. Subsequently, 4 mL of 20% (w/v) TCA containing 0.5% TBA was poured into every 1 mL of the supernatant. This mix was excited at 95 • C for 30 min, chilled speedily, and centrifuged for 15 min at 10,000× g. The absorbance was calculated at 532 and 600 nm, and molar absorptivity of 155 mM −1 cm −1 was applied to calculate the MDA concentration.
Additionally, the protein carbonyl group (nM/g FW), as an eventual result of protein oxidation, was estimated via colorimetrically following Levine et al.'s [105] protocol. Plant homogenates (0.5 mL) were mixed with 0.5 mL of 2,4-dinitrophenylhydrazine solution in a tube and incubated at laboratory temperature for 2 h with an occasional vortex. Subsequently, 20% trichloroacetic acid (0.05 mL) was decanted followed by centrifugation. The supernatant was removed, and the pellets were washed 3 times with ethyl acetate solution (1 mL). The protein, free of unreacted reagent, was suspended in guanidine hydrochloride solution (0.6 mL). Carbonyl concentration was calculated from maximum absorbance (390 nm) using a molar extinction coefficient of 22,000 L/(mol·cm).
Cell membrane permeability (MP) was estimated following the technique of Tariq et al. [103] with slight adaptations. Plant segments were allowed to deposit in 10 mL of distilled water for 6 h and then preliminary electrical conductivity (EC1; Hanna Instruments, UK) was recorded. The samples were heated at 1000 C for 15 min and chilled to laboratory temperature, and subsequently, conductivity was calculated once more (EC2). The MP% was established following this equation: MP% = (EC1/EC2) × 100.

Antioxidant Enzyme Assay
Antioxidant enzyme activity (unit mg −1 protein) was estimated in the main shoot tips (with two leaves). Shoot tips (0.5 g) were triturated in sodium phosphate buffer (50 mM, pH 7.0) with 1% soluble polyvinylpyrrolidine, and then centrifuged at 20,000× g for 15 min. Finally, the supernatant was utilized for the evaluation of the antioxidant enzyme activity.
Peroxidase (EC 1.11.1.7) was determined as guiacol-stimulated peroxidation of H 2 O 2 [85] with a few modifications. Phosphate buffer (50 mM) was utilized for the extraction of POD followed by centrifuging. The assay mixture was composed of phosphate buffer, H 2 O 2 , guaiacol, and enzyme extract. The OD at 436 nm was measured, and the molar extinction coefficient of 26.6 mM −1 cm −1 was achieved for the enzyme activity measurement (1 unit = 1 µM H 2 O 2 decline min −1 ).
Catalase (EC 1.11.1.6) was anticipated by assessing the prime speed of H 2 O 2 defeat depending on the modified procedure of Chrysargyris et al. [106]. About 100 µL of enzyme extract was poured into 50 mM K phosphate buffer (pH 7.0) and 10 mM H 2 O 2 for a total amount of 3 mL. Enzyme activity was considered at 240 nm for 2 min. The extinction coefficient of 39.4 mM −1 cm −1 was exploited to calculate CAT activity (1 unit = 1 mM of H 2 O 2 decline min −1 ).

Antioxidant Metabolites
The scheme utilizing 2.6-dichlorophenol indophenol and Folin-Ciocalteu techniques was occupied for assessing the levels of ascorbic acid (AsA, mg/g FW) and total phenol concentration (mg gallic/g DW) in the shoot tips, respectively [97].
The flavonoids' concentration (Flav, mg quercetin g −1 FW) was calculated by the aluminum chloride (AlCl 3 ) procedure according to Sevket et al. [107]. A 1.0 mL portion of plant extract was placed in a 10 mL volumetric flask, distilled water (4 mL) was poured, 0.3 mL of sodium nitrite (NaNO 2 ) was added, and the mixture was mixed carefully. After 5 min, 3 mL of AlCl 3 was poured and the solution was mixed thoroughly for 5 min; then, we added 2 mL of sodium hydroxide, 2.4 mL of distilled water was added, and absorbance was calculated at 510 nm with quercetin utilized as the standard for calibration.

Yield and Oil Percentage
At harvesting, seed yield/plant, seed index (100 seed weight, g), oil percentage, and oil yield/plant were determined. The oven-dried powdered seeds were extracted for 6 h in a Soxhlet extractor with n-hexane as a solvent, and the oil-containing hexane was filtered by the Whatman No. 1 filter paper, and subsequently excited at 60 • C to get rid of the final traces of the solvent for about 2-3 h [108].

Fatty Acid Profiles
For the control, salinity, and Bi+Mt treatment under salinity treatment, the seed oil was collected for determination of its constituents. Fatty acid methyl esters (FAME) were propagated from lipid by using a quick technique along with the ISO 12966-2 protocol. FAME was produced by trans-esterification with methanolic potassium hydroxide as a transitional phase before saponification took place. Around 0.1 g of the oil was cited in a 5 mL screw-top test tube, and isooctane (2 mL) was poured into the tube and the tube was shaken. Methanolic potassium hydroxide solution (0.1 mL, 2 N) was set on the cap fixed with a PTEE joint, the cap was tightened and shaken dynamically for the 30 s, and the tube was left to stratify while awaiting the upper solution and then was poured. The isooctane solution is appropriate for injection into the gas-liquid chromatography (GLC). GLC equipped with a DB-23 column (60 m × 0.25 mm × 0.23 µm). The carrier gas was N 2 with a flow speed of 1.5 mL/min and a splitting ratio of 1:50. The injector temperature was 250 • C and that of the flame ionization detector (FID) was 280 • C. The peaks were identified by comparing the retention times gained with standard methyl esters.

Statistical Analysis
Data were examined by one-way analysis of variance (ANOVA). Means were compared by the multiple mean comparison statistic and Tukey's Honestly Significant Difference (HSD) test with COSTATC statistical package (CoHort software, 2006; North Carolina, USA). Statistical significance was set at p < 0.05. Data were presented as means ± standard error (SE) of five independent biological replications.

Conclusions
Exogenous application of Bi and/or Mt will potentially lighten the oxidative stress by improving the antioxidant power of plants as well as decreasing Na + accumulation. Moreover, these treatments can lessen salt stress by encouraging the buildup of osmoprotectants, i.e., soluble sugars and proline. The outcome offers a speculative base for Bi and/or Mt to alleviate salt stress. Extra studies are necessary to understand the accurate functions of Bi and/or Mt under salinity. As Mt and/or Bi are secure for animals and humans and low priced, so a conditioning method utilizing them as plant activators is possibly a consistent, reasonable, and cost-effective means for producing borage plants in saline soil.