Improvement of Germination and Early Growth of Radish (Raphanus sativus L.) through Modulation of Seed Metabolic Processes

Radish (Raphanus sativus L.) is a vegetable cultivated worldwide because of its large succulent hypocotyls. The priming method initiates metabolic processes at early stages and regulates the metabolic events in seed necessary for germination. This research was conducted to examine the influence of various priming treatments on physiological performance (germination, growth, lipid peroxidation, primary and secondary metabolism) and antioxidant activity of radish seedlings. On the basis of germination and growth characteristics, vigor index, and relative water content in leaves, it was confirmed that priming treatments with 0.01% ascorbic acid (AA) and 1% KNO3 improves the initial stages of radish development. Furthermore, the efficiency of AA as a priming agent was confirmed through the reduction of malondialdehyde (MDA) level compared to unprimed seedlings. On the other hand, hormopriming with indole-3-acetic acid (IAA) significantly increased the concentration of photosynthetic pigments and total soluble leaf proteins compared to non-primed seedlings. The highest content of total phenolic compounds, including flavonoids, were obtained after hormopriming with 1 mM IAA and halopriming with 1% MgSO4. On the basis of the percentage of inhibition of DPPH radicals, it was confirmed that treatments with IAA and AA can improve the antioxidant activity of radish seedlings. This study provides useful information regarding the possibilities of pregerminative metabolic modulation through the seed priming for the biochemical and physiological improvement of radish, and this topic should be further investigated in order to determine the potential use of AA and IAA as suitable priming agents in radish commercial production.


Introduction
Sustainability and improvement of crop production is essential to meet consumer demands [1]. Seed germination and seedling growth are two critical points for the growth of any crop [2,3]. In order to provide high-quality seeds for successful agricultural production, various methods and techniques have been developed to improve the seed germination process, and among them, the priming method is one of the most effective since it improves seed performance and enables faster and synchronized germination [4,5]. On the basis of seed hydration, this method initiates metabolic processes in the early stages of germination but does not permit radicle protrusion. Activated metabolic processes are interrupted by placing the seeds on desiccation until they are placed again in a humid environment [5,6]. Major cellular processes are initiated as a consequence of rehydration, such as de novo ATP production, nucleic acid and protein synthesis, phospholipid and sterol accumulation, activation of antioxidant mechanisms, and DNA repair [7]. Consequently, primed seed

Seed Moisture Content
To exclude the possibility that physiological performances of radish seedlings varied due to different moisture content in seeds after application of various priming agents, the seed moisture content was measured (both after priming for 24 h and after desiccation of primed seeds prior to germination) and compared with the control (unprimed seeds). The results are presented in Figure 1. The moisture content of unprimed radish seeds was 4.34%. The moisture content of seeds immediately after priming was nearly 10 times higher compared to the control seeds and tended to be similar, without significant differences between the priming agents applied. Additionally, the moisture content of seeds primed with various agents (after desiccation) was without significant differences among treatments applied. Still, the moisture content of primed seeds prior to germination was almost doubled compared to the control (unprimed) seeds. The moisture content of radish (R. sativus) seeds after priming for 24 h and after desiccation of primed seeds for 48 h prior to germination; control-unprimed seeds. The values represent the means of five replicates ± standard error. Small alphabetical letters (a,b) above the means show the differences (p ≤ 0.05) between primed seeds after desiccation and unprimed seeds, while capital alphabetical letters reveal the differences (p ≤ 0.05) between seeds immediately after 24 h of application of different priming agents by Tukey's test.

Seed Germination Characteristics
The obtained results for the germination percentage (GP), mean germination time (MGT), and rate of germination (RG) are shown in Table 1. All applied treatments had stimulating effects on all the tested germination characteristics of radish seeds. The highest percentage of germination was recorded after treatment with AA (GP = 89.92%), which at the same time had a pronounced effect on germination rate. The most significant effect on mean germination time and rate of germination was achieved by H2O2 treatment (MTG = 1.50; RG = 67.12).

Seed Germination Characteristics
The obtained results for the germination percentage (GP), mean germination time (MGT), and rate of germination (RG) are shown in Table 1. All applied treatments had stimulating effects on all the tested germination characteristics of radish seeds. The highest percentage of germination was recorded after treatment with AA (GP = 89.92%), which at the same time had a pronounced effect on germination rate. The most significant effect on mean germination time and rate of germination was achieved by H 2 O 2 treatment (MTG = 1.50; RG = 67.12).

Growth Characteristics and Vigor Index
Influence of applied priming treatments on growth characteristics (shoot length, root length, fresh and dry weights) of radish are shown in Table 2. Compared to the control, the most significant effect on shoot length was observed after priming treatment with GA 3 (shoot length = 5.73 cm). The most prominent effect on root length was observed after treatment with AA (root length = 10.85 cm), whereas for fresh and dry weights, the highest values were recorded after halopriming with KNO 3 (0.0931 g and 0.0081 g, respectively). Table 2. Effect of different priming treatments on shoot and root length (cm), fresh and dry weight (g), leaf relative water content-RWC (%), seedling weight vigor index (SWVI), and seedling length vigor index (SWVI) of radish (R. sativus). Since increased vigor index values of seedlings can improve the initial stages of growth and development, the evaluation of vigor tests-seedling weight vigor index (SWVI), and seedling length vigor index (SWVI)-was performed. The results of these vigor tests (SLVI and SWVI) are presented in Table 2. On the basis of SLVI values, stimulating effects for each of the applied treatments can be seen, whereas the most significant effect was achieved with AA (SLVI = 1337.11). The results obtained for SWVI showed that the highest values were recorded after osmo-and halopriming without significant differences between these treatments.

Relative Water Content (RWC)
Leaf relative water content (RWC) varied depending on the treatment applied but without significant differences among applied treatments and unprimed seeds (Table 2). Still, after halopriming with KNO 3 , a slight increase in relative water was observed (RWC = 96.59%).

Concentration of Photosynthetic Pigments
Concentration of photosynthetic pigments in radish leaves depended on the applied priming treatment (Table 3). Observed through the concentration of total chlorophyll, chlorophyll a, and chlorophyll b, significant similarities were noticed in the synthesis of these photosynthetic pigments in relation to the applied priming treatment ( Table 3). The highest concentration for all pigments was measured after seed priming with IAA, and the values were significantly different compared with both control and other treatments. The same trend was observed for the concentration of carotenoids. Oppositely, seed priming with H 2 O 2 caused a significant decrease in the concentration of the examined photosynthetic pigments.

Concentration of Soluble Proteins
The influence of different priming agents on the concentration of total soluble proteins is shown in Figure 2, and it was noticed that seed priming had a stimulating effect on the concentration of soluble proteins. The highest content of total soluble proteins was measured during seed priming with IAA (31.30 mg g −1 FW).

Concentration of Soluble Proteins
The influence of different priming agents on the concentration of total soluble proteins is shown in Figure 2, and it was noticed that seed priming had a stimulating effect on the concentration of soluble proteins. The highest content of total soluble proteins was measured during seed priming with IAA (31.30 mg g −1 FW).

Figure 2.
Influence of different priming treatments on the concentration of total soluble proteins in leaves of radish (R. sativus) seedlings; the values represent the means of six replicates ± standard error. Different letters indicate significant differences (p ≤ 0.05) between treatments according to Tukey's test.

Malondialdehyde Content (MDA)
MDA content is an important indicator of the degree of lipid peroxidation that causes cellular dysfunction. The examined radish seedlings showed significant differences in terms of MDA concentration depending on the applied treatment ( Figure 3). All tested priming agents achieved positive effects since the MDA content was significantly lower after priming compared to seedlings estimated from nonprimed seeds. The obtained Figure 2. Influence of different priming treatments on the concentration of total soluble proteins in leaves of radish (R. sativus) seedlings; the values represent the means of six replicates ± standard error. Different letters indicate significant differences (p ≤ 0.05) between treatments according to Tukey's test.

Malondialdehyde Content (MDA)
MDA content is an important indicator of the degree of lipid peroxidation that causes cellular dysfunction. The examined radish seedlings showed significant differences in terms of MDA concentration depending on the applied treatment ( Figure 3). All tested priming agents achieved positive effects since the MDA content was significantly lower after priming compared to seedlings estimated from nonprimed seeds. The obtained results indicate the ability of applied priming agents to reduce lipid peroxidation and increase the stability of cell membranes, with the most favorable effect observed after priming with AA (MDA content was about six time lower compared to unprimed seedlings).

Concentration of Total Phenolic Compounds and Flavonoids
The applied priming treatments had a significant effect on the synthesis of total phenolic compounds, as well as flavonoids. The measured concentrations of total phenolic compounds in radish extracts were in the range of 25.92 to 29.92 mg of GA g −1 of extract ( Figure 4). All applied treatments induced synthesis of phenolic compounds, whereas the

Concentration of Total Phenolic Compounds and Flavonoids
The applied priming treatments had a significant effect on the synthesis of total phenolic compounds, as well as flavonoids. The measured concentrations of total phenolic compounds in radish extracts were in the range of 25.92 to 29.92 mg of GA g −1 of extract ( Figure 4). All applied treatments induced synthesis of phenolic compounds, whereas the highest values were recorded after halopriming with MgSO 4 and hormopriming with IAA (29.29 and 28.72 mg of GA g −1 of extract, respectively). Influence of different priming treatments on malondialdehyde (MDA) content in leaves of radish (R. sativus) seedlings; the values represent the means of six replicates ± standard error. Different letters indicate significant differences (p ≤ 0.05) between treatments according to Tukey's test.

Concentration of Total Phenolic Compounds and Flavonoids
The applied priming treatments had a significant effect on the synthesis of total phenolic compounds, as well as flavonoids. The measured concentrations of total phenolic compounds in radish extracts were in the range of 25.92 to 29.92 mg of GA g −1 of extract ( Figure 4). All applied treatments induced synthesis of phenolic compounds, whereas the highest values were recorded after halopriming with MgSO4 and hormopriming with IAA (29.29 and 28.72 mg of GA g −1 of extract, respectively).

Antioxidant Activity
The percentage of DPPH inhibition, determined spectrophotometrically through the activity of the radish extracts in the process of removing DPPH radicals, differed significantly depending on the concentration of the plant extract and the treatment applied (Ta-

Antioxidant Activity
The percentage of DPPH inhibition, determined spectrophotometrically through the activity of the radish extracts in the process of removing DPPH radicals, differed significantly depending on the concentration of the plant extract and the treatment applied (Table 4). Radish extracts were able to remove DPPH radicals, and the efficiency depended on the concentration, which can be explained by the dilution effect. The highest percentage of DPPH inhibition was observed for extracts obtained after seed priming with KNO 3 (67.32% of DPPH inhibition for 500 µg mL −1 of plant extract) and was followed by AA (66.91% of DPPH inhibition for 500 µg mL −1 plant extract) and IAA treatments (66.81% of DPPH inhibition for 500 µg mL −1 plant extract). Between these treatments, obtained values were without significant differences. The lowest percentage of inhibition was recorded after treatment with H 2 O 2 (61.69% of DPPH inhibition for 500 µg mL −1 plant extract).

Discussion
Radish, as a vegetable of great economic importance, contains essential nutrients for human nutrition and health [13], and cultivation of this vegetable is intensified worldwide. Seed germination is considered as an initial and critical determinant of crop success. Therefore, stimulation of seed germination can be used as a basic means to increase the yield of horticultural crops [25]. In this regard, seed priming is considered as an effective practice to improve overall seed germination and germination uniformity by using various methods and chemicals [26,27]. Seed priming can greatly improve the performance of radish seeds, which is of global economic importance. Efficient, fast, and uniform seed germination is the first and most important step towards the success of any crop in commercial crop production [3]. If we consider the overall impact of the applied treatments on the germination characteristics, the results of this study showed remarkable improvement of radish germination process after seed priming. This could be related to the increase in moisture content in the seeds after priming [28]. The most pronounced effect on total germination was achieved with ascorbic acid (AA), while after H 2 O 2 treatment, seeds germinated faster. According to Alves et al. [29], a positive effect of AA is as a result of its influence on numerous processes that improve the physiological activity of embryos and future seedlings. The positive effects of the application of H 2 O 2 as a priming agent on germination dynamics have been confirmed previously [30,31]. Still, the dual role of hydrogen peroxide as a toxic molecule on one hand and as a signal molecule on the other should be considered [30], since in this study, H 2 O 2 as a priming agent caused decrease of photosynthetic pigments, flavonoids, and total antioxidant activity.
In addition to its improvement of germination characteristics, AA was the most significant priming agent for the improvement of radish growth, with an overall better stimulating effect on root elongation. Vitamins are compounds that have role of bioregulators and hormone precursors, and numerous physiological processes such as water absorption and cell division depend on vitamin availability [32]. AA is included in multiple enzymatic reactions, and it is essential to many aspects of plant growth including the regulation of cell cycle, cell division, and embryo development. The changes in ascorbic acid levels substantially alter the plant gene expression profile, raising the possibility of multiple reactions following alterations to AA content [33]. Improved root growth from seeds primed with ascorbic acid suggests that ascorbic acid is able to stimulate some cellular processes, including cell division and nutrient absorption in plant cells [34]. Similarly, a superior effect of KNO 3 on radish mass was observed. Potassium nitrate, due to its nutritional value, in addition to acting as a salt, also has the property of a growth regulator, with a significant impact on plant mass [35]. Moaaz et al. [24] confirmed that KNO 3 as a priming agent can significantly improve plant mass and growth. Moreover, a marked effect of these treatments in terms of vigor index was observed. Evaluation of seedling vigor index to determine seed quality is an important parameter since the seedling vigor index determines the viability of seeds as well as the ability of seeds to produce quality plants under conditions similar to those in nature [36]. High values of the seedling vigor index imply that plants will have the fastest possible germination, high percentage of germination, and higher seedling biomass and leaf area [37]. On the basis of the results of the seedling vigor index and early growth, it can be expected that the initial stages of growth and development of radish seeds will be better after priming with AA and halopriming with KNO 3 .
Photosynthesis is a fundamental physiological process, and the parameters of photosynthesis are inextricably linked with the content of chloroplast pigments, primarily chlorophyll a, chlorophyll b, and carotenoids [38]. Low concentration of photosynthetic pigments can directly limit photosynthetic potential, and thus primary production [39]. Priming improves the concentration of photosynthetic pigments in radish leaves since seed priming resulted in a higher concentration of photosynthetic pigments compared to those developed from untreated seeds, whereas IAA treatment played a dominant role. IAA is known to stimulate plant growth, with a significant role in the process of photosynthesis and pigment formation [40]. The significant role of IAA in improving photosynthetic activity is also emphasized by Rhaman et al. [41]. Zhao et al. [42] confirmed that priming with IAA directly improved photosynthesis capacity, increasing stomatal conduction and intercellular CO 2 concentration. The obtained results are in accordance with those from previous studies, where it was confirmed that priming agents under both favorable and unfavorable environmental conditions improve the concentration of photosynthetic pigments [43][44][45][46].
Secondary metabolites of phenolic nature, including flavonoids, are the main compounds responsible for the defense mechanism of plants [47], but also for the improved nutritional values of crops because of their well-known antioxidant activities. It has been previously confirmed that the concentration of phenolic compounds in radish depends on the particular radish variety and the influence of methods used to improve radish characteristics [13,48]. In this study, the use of hormone priming with IAA and osmopriming with MgSO 4 had a superior effect in terms of total phenolic content and flavonoid concentration. The stimulatory effect of magnesium ions (Mg 2+ ) on the synthesis of phenolic compounds was confirmed by Farzadfar et al. [49]. A similar observation on the multiple importance of auxin, including the effect on the biosynthesis of branched compounds such as phenolic compounds, were noted by Baliyan et al. [40]. Accordingly, it can be assumed that priming agents would play a key role in the defense mechanism of radish seedlings by increasing the concentration of total phenolic compounds including flavonoids. Furthermore, the positive effects of the named priming agents were observed in terms of protein content, as well as flavonoids. An increase in the total soluble protein content may be associated with improved physiological activities, mainly due to stimulation of the radish protein biosynthesis process [50].
Antioxidant compounds are naturally presented in plants and can be used preventively in the diet in order to prevent oxidative stress. Previous research has confirmed the antioxidant properties of radish [48,51]. Post-priming dehydration can lead to a number of cellular and biochemical processes, including activation of antioxidant defense systems [2], and in this study, seed priming with KNO 3 , AA, and IAA increased the ability of radish extracts to reduce free DPPH radicals, i.e., increased the total antioxidant activity of radish seedlings. According to Singhal et al. [52], the application of halopriming increases antioxidant capacity, probably acting as signaling molecules that drive several metabolic pathways, such as stomatal openings, abscisic acid production, synthesis, and increased antioxidant enzyme activity. Molecules of antioxidants such as ascorbic acid applied in the form of priming have been found to reduce and alleviate oxidative stress of plants to significant levels [53]. Madany et al. [54] confirmed that priming seeds with IAA has great potential to improve overall antioxidant capacity, especially at levels of oxidative stress. Similarly, Hamid et al. [55] and Ahmad et al. [56] documented that priming wheat and corn seeds resulted in an improved antioxidant defense system of their seedlings. Seed priming stimulates plants to better regulate oxidative stress, indicating the important role of priming in managing abiotic stress responses in crops.
The regulatory effect of seed priming is closely related to the activation of the antioxidant protection system, the removal of reactive oxygen species (ROS), or the increased biosynthesis of phenolic compounds [3]. In addition, MDA content, as a final byproduct of lipid peroxidation, represents an indicative measure of oxidative damage [29]. In general, primed seed produced a lower content of MDA, which keeps the cell membrane stable and reduces ROS production by detoxifying them into non-toxic molecules, resulting in reduced oxidative stress and reduced membrane damage [52]. The effectiveness of AA priming agents in reducing ROS levels and the avoiding of lipid peroxidation was confirmed by Alves et al. [29]. In the defense of plants against oxidative stress, vitamins with their antioxidant properties play an important role as free radical scavengers. Ascorbic acid is affiliated with chloroplasts in which the effect of oxidative stress on photosynthesis is mitigated. AA is one of the non-enzymatic antioxidant compounds serving both as an electron donor to reduce the accumulation of ROS as well as the reaction substrate within the enzymatic cycle [32]. Auxins, especially IAA, can control the growth and development of plants. In addition to the well-known roles in plant elongation, apical dominance, or rhizogenesis, recent investigations suggest that auxin has influences on pigment and protein content, as well as increased concentrations of secondary metabolites where increased content of pigments under IAA treatments could be related to the protection of the leaf photosynthetic apparatus [57]. It is believed that the increase in antioxidant activity occurs when using endogenous IAA, where the hormone treatment stimulates plant secondary metabolism, thereby resulting in a higher concentration of secondary products such as phenols and flavonoids, which have a consequential impact on oxidative status in plants [58].

Experimental Design
Seeds of red radish (Raphanus sativus L. var. Saxa) were obtained from commercial sources ("Morpho d.o.o." Belgrade). The seed preparation and priming method were conducted according to the work of Kanjevac et al. [59] with minor modifications. Applied treatments are presented in Table 5. In brief, the surface-sterilization was performed with sodium hypochlorite, and sterilized seeds were primed with the appropriate treatment. For each treatment, 30 seeds were soaked in 10 mL of the appropriate solution for 24 h and then subjected to a desiccation process for 48 h at room temperature. Unprimed seeds were used as a control. Primed and unprimed seeds were placed in Petri dishes with filter paper (Whatman No. 1), soaked in 7 mL of distilled water, and incubated in a growth chamber (temperature 23 ± 2 • C, photoperiod 16/8 h, 60% humidity). For every treatment, germination was counted to the final count until no further germination occurred. The growth characteristics and physiological performances were evaluated 10 days after seed germination.

Seed Moisture Content
To estimate the moisture content of radish seeds during the different stages of seed priming, a low constant temperature oven method at 101-105 • C for 17 h was applied [60]. The same method was applied to measure the moisture content of unprimed seeds. In brief, seed moisture content was estimated for (1) seeds immediately after 24 h of application of different priming agents, (2) seeds after application of different priming agents followed by desiccation prior to germination, (3) unprimed seeds prior to germination. The seed moisture content (%) was calculated using the following equation: Seed moisture content = Weight of fresh seeds − Weight of dry seed Weight of fresh seeds × 100

Germination Characteristics
The germination characteristics of the tested species under the influence of different treatments were examined by measuring the total germination percentage (GP), mean germination time (MTG), and rate of germination (RG). Seeds were considered germinated after radicle appearance (at least 2 mm). Values were calculated according to the work of Bewley and Black [61] and Jakovljević et al. [62] on the basis of the following equations:

GP =
Total seeds germination Total number of planted seeds × 100 MTG = ∑ n i × t i ∑ n i ; n i = number of newly germinated seeds; t i = days from start of the experiment to the observation. RG = MGR × 100; MGR = mean germination rate, the reciprocal of the mean germination time.

Growth Characteristics and Vigor Index
Index for evaluation of the seedling vigor was calculated according to the work of Bojović et al. [34] on the basis of the following equations: Seedling Length Vigor Index (SLVI) = (Mean shoot length + Mean root length) × FGP Seedling Weight Vigor Index (SWVI) = Mean seedling weight × GP GP = Total germination percentage (%)

Relative Water Content
The leaf relative water content (RWC) is defined as the amount of water in plant leaves in relation to the state of full turgidity. The relative water content in the leaves (%) was measured and calculated according to the work of Dastborhan et al. [63] on the basis of the following equation: RWC = fresh weitgh − dry weitgh turgid weitgh − dry weitgh × 100

Measurement of Photosynthetic Pigments
The influence of the applied treatments on the concentration of photosynthetic pigments was examined by measuring the content of chlorophyll a (Chl a), chlorophyll b (Chl b), total chlorophyll (T-Chl, Chl a + b), and carotenoids (Cx + c). The preparation of plant material was performed according to the work of Bojović and Stojanović [64]. Briefly, 0.5 g of fresh leaf tissue was homogenized with the addition of 10 mL of 80% acetone and then centrifuged at 2500 rpm for 5 min. Concentrations of photosynthetic pigments were calculated according to the work of Wellburn [65] and expressed in relation to fresh weight (mg g −1 FW): Chl

Total Soluble Protein Concentration
The concentration of total soluble proteins in radish leaves was determined by the work of Lowry et al. [66] with bovine serum albumin (BSA) as standard. Values were calculated and expressed in relation to the fresh weight (mg g −1 FW).

Malondialdehyde Content
The determination of lipid peroxidation marker MDA was determined by the thiobarbituric acid (TBA) reaction in which mixture included enzyme solution, 0.5% TBA, and 20% trichloroacetic acid, while the absorbance was determined spectrophotometrically at 532 and 600 nm [67]. MDA content was expressed as nM g −1 FW.

Preparation of Plant Extracts
Juvenile plant parts (radish shoots) were sampled after 10 days from seed germination. The sampled plant material was dried at room temperature, at an air humidity of 55-60%, for 7 days. After that, the dry plant material was crushed to obtain plant powder, and plant extracts were prepared (20 mL of methanol for 1 g of plant powder). After 48 h, the extracted samples were filtered and then evaporated. To determine the concentration of total phenolic compounds and total flavonoids, as well as to measure the total antioxidant activity, a methanolic extract solution at a concentration of 1 mg/mL was used.

Determination of Total Phenolic Compounds
The amount of total phenolic compounds was determined according to the work of Singleton et al. [68] using gallic acid as a standard. Briefly, 0.5 mL of plant extract, 2 mL of 7.5 NaHCO 3 , and 2.5 mL of 10% Folin-Ciocalteu reagent were taken to prepare the reaction mixture. The samples were incubated for 15 min at 45 • C, and the absorbance was determined at a wavelength of 765 nm. The concentration of total phenols was calculated on the basis of the measured absorbance and calibration curve for gallic acid and was expressed in gallic acid equivalents (mg of GA g −1 of extract).

Determination of Total Flavonoids
The concentration of flavonoids was determined according to the work of Quettier-Deleuet al. [69] on the basis of the reaction of flavonoids with AlCl 3 , and rutin was used as a standard. The reaction mixtures were prepared with 1 mL of plant extract and 1 mL of 2% AlCl 3 dissolved in methanol. The samples were incubated for one hour at room temperature, after which the absorbance was determined at a wavelength of 415 nm. Calculation was based on the measured absorbance and the calibration curve for rutin as a standard, and the concentration of flavonoids was expressed in rutin equivalents per milligram of extract (mg of RU g −1 of extract).

Evaluation of Antioxidant Activity
The total antioxidant activity was determined with the spectrophotometric method based on measuring the degree of neutralization of free 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical [70]. For each sample, starting from the extract concentration of 1 mg/mL, dilution series were made to obtain concentrations of 500, 250, 125, 62.5, 31.25, 15.62, 7.81, 3.90, 1.99, and 0.97 µg mL −1 . Diluted solutions (1 mL each) were mixed with 1 mL of DPPH radical solution (80 µg mL −1 ). The prepared samples were incubated for 30 min at room temperature in the dark, after which the absorbance was determined at a wavelength of 517 nm. The percentage inhibition of DPPH radicals was expressed on the basis of differences in the absorbance of samples of plant extracts and control samples (samples without extract solution):

% inhibition =
A of control − A of sample A of control × 100

Statistical Analysis
The experiments were performed in at least five repetitions, and data are presented as mean ± SE (standard error). The program SPSS v.21 for Windows was used for the statistical evaluation of the results with an analysis of variance (ANOVA) and Tukey's multiple range tests (p ≤ 0.05).

Conclusions
Seed priming is a promising tool for improvement of the radish seed germination process, as well as early seedling growth. Radish responded positively to all applied priming treatments, which showed different efficacy depending on the tested characteristics. It was observed that seed germination, radish growth performance, and level of malondialdehyde were improved by priming with AA. In addition, a significant influence of applied priming agents on the physiological processes of seedlings was shown, whereas hormopriming with IAA improved the concentration of photosynthetic pigments and proteins, as well as total phenolic content and flavonoid concentration. At the same time, priming with IAA was effective in the improvement of total antioxidant activity compared to unprimed radish seedlings. Because the seed priming technique is a simple, fast, inexpensive method and less labor-intensive than conventional strategies, the agricultural industry can easily adopt it. Further studies should include an assessment of radish growth under adverse conditions to show whether the priming method could improve the production of this valuable crop under diverse cultivation conditions.