Variation of the Chemical Composition of Essential Oils and Total Phenols Content in Natural Populations of Marrubium vulgare L.

Marrubium vulgare is a valuable source of natural bioactive molecules with high preventive and therapeutic effectiveness. Therefore, this study aimed to study the chemical polymorphism of natural populations of M. vulgare in Tunisia by quantitative chemical markers and the estimation of divergence between populations. Phytochemical analyses of the eight natural populations of Tunisian Marrubium vulgare prospected in different bioclimatic stages, revealed 42 compounds of essential oils representing 96.08% to 100% of the total oil. Hydrocarbon sesquiterpenes were the main fraction of all the populations studied and β-bisabolene was the major compound (from 30.11% to 71.35% of the total oil). The phytochemical investigation of the M. vulgare plant indicated the presence of essential oil with significant percentages of phenolic compounds. A significant quantitative and qualitative variation in the essential oils is detected for both major and minor compounds. The principal components analysis (PCA) performed in the single and combined traits provides a good distinction among populations, not according to their geographical and/or bioclimatic origins. Moreover, the phytochemical analysis of the leaves showed that the Tunisian populations, i.e., the populations of Kasserine, Kef, and Beja, were very rich in phenolic compounds (from 20.8 to 44.65 mg GAE/g DW). Flavonoids compounds were also the main class of total polyphenols present in all the tested populations (from 8.91 to 37.48 mg RE/g DW). The quantitative genetic diversity estimated by the population’s structure, based on PCA analysis, was an adaptation to the changes in the environmental conditions. Overall, our study indicated that natural populations of M. vulgare had different chemotypes of essential oils and they were rich in phenolic compounds, particularly flavonoids, which opens a new prospect for industrial use and differential exploitation of this species.


Introduction
Food flavoring, fragrance, cosmetics, and pharmaceutical companies have been in high demand for the essential oils of plants and their other secondary metabolism products in recent years, due to a rising customer interest in natural components [1]. Food, medications, and perfumes are just a few of the things for which plants have been used. They were tested to determine if they can be used to treat a range of infections and to protect foods from oxidative damage [2]. Lamiaceae species produce a great variety of secondary compounds, especially essential oils, which are found in prolruding multicelled glandular trichomes and produced in the glandular hairs on the surfaces of leaves and inflorescences [3]. In Tunisia, the Lamiaceae family is much exploited for its therapeutic virtues. In addition, about 70% to 90% of the species originate from spontaneous populations. Nevertheless, excessive exploitation of the species from this family, as for all aromatic and medicinal plants, has led to a serious reduction of population numbers and the destruction of their habitat [4]. This family is well known with two major series of genera: oil-rich and oil-poor species, and several members of the family are used as sources of essential oils. Among the species of this family, the genus Marrubium (Lamiaceae), includes roughly 97 species found in temperate climates, as well as the Mediterranean, Asia, America, and Australia [5]. The Marrubium genus grows spontaneously in all countries from north to south; it is represented by five species including M. vulgare L., M. Aschersonii P Magnus, M. alysson, M. supinum L., and M. deserti of Noah [6]. This plant, also known as "horehound" in Europe and "Om Rubia" in Tunisia, grows wild in dry sandy soils and wastelands, and is widely used as a raw material in the herbal extracts and beverage industries. It is also well used in traditional medicine in phytotherapy [7]. M. vulgare is often an important source for the food and pharmaceutical industry. For example, in Tunisia, it is well used in traditional medicine as a hypotensive, a hypoglycemia substance, and in asthma and lung diseases [8]. Tonic, aromatic, stimulant, expectorant, diaphoretic, and diuretic characteristics are all found in M. vulgare. Phytochemicals found within it include phenyl propanoids, phenolic compounds, and diterpenic lactones. The marrubiin is the most active diterpenoid responsible for the therapeutic properties observed from these species [9]. It displays a large variety of biological activities including antimicrobial [10], cardioprotective [11], antitumor [12], immunomodulatory [13], antioxidant [14], antidiabetic [8], antiprotozoal, gastroprotective antiviral [14], antihepatotoxic [15], vasorelaxant [16], antioedematogenic [17], insecticidal [3], and antihypertensive activities [18]. High antibacterial, antifungal, and cytotoxic properties can be explained by the high levels of essential oils in Marrubium species [19]. The leaves and flowering stems are used as diuretics, antispasmodics, antiseptics, and antidiabetics. Essential oils are another important secondary metabolite since they are highly bioactive in some situations, readily available in tropical countries, and commercially viable. According to Zarai et al. (2011) [20], M. vulgare has a moderate amount of essential oil, about 0.1%.
Limited studies on the chemical composition, and the antioxidant and antimicrobial effects of Marrubium vulgare L. have been investigated in Tunisia [7]. Unfortunately, in Tunisia, the natural users of this heritage (M. vulgare) often exploit the spontaneous populations, without interest for their durable management and their conservation. Nevertheless, the excessive exploitation and the habitat destruction of this species result in important genetic erosion, characterized by the reduction of the population size, which can lead to the decrease of gene flow levels between them. For this reason, M. vulgare deserves programs to safeguard the natural populations to assure their durable usage, as with the majority of other PAM, which represent a bank of natural bioactive molecules with high preventive and therapeutic effectiveness. In this regard, any politics of aromatic and medicinal genetic resources conservation necessitates a description, as exhaustive as possible, of the chemical and genetic divergence of natural populations. As far as we know, no research has been published about the genetic diversity of M. vulgare. Thus, the objective of the present work was to study the chemical polymorphism of natural populations of M. vulgare in Tunisia. Moreover, to identify the best habitat to achieve the highest antioxidant properties among the different habitat conditions. In this regard, the quantitative chemical markers (essential oil, terpene composition, total polyphenol, and flavonoid content) and the estimation of the divergence between populations could help to develop rational exploitation and conservation programs.

Plants Materials
The fresh leaves of eight populations of M. vulgare prospected in Tunisia in March 2018 were collected during the flowering stage. These eight populations represented most of the geographic range covered by the species in Tunisia. The characteristic of each station is defined by Emberger (1966) [21] (Table 1).

Essential Oil Isolation
About 250 g of fresh leaves and flowers of M. vulgare were separately submitted to hydrodistillation for 3 h, using a Clevenger-type apparatus (British Pharmacopoeia, 1980). Leaves were placed in 665 mL of distilled water, which represents a load report of 2/3 (0.01-0.04% yield). Anhydrous sodium sulphate was used to eliminate water traces from the essential oil. The essential oil was stored at +4 • C until tested. The condition of the culture, the extraction method, and stockage were the same for all the populations analyzed.

Gas Chromatography and Gas Chromatography-Mass Spectrometry
The essential oil composition was determined by a Varian Star 3400 (Les Ulis, France) Cx gas chromatograph, equipped with a flame ionisation detector (FID) and a DB-5 (30 m × 0.25 mm; film thickness 0.25 µm) capillary column. The oven temperature gradually rose from 60 • C to 260 • C, with a gradient of 5 • C/min and 15 min isothermal at 260 • C, and finally rose to 340 • C at 40 • C/min. Helium (purity 99.99999%) was used as the carrier gas at 1 mL/min. The total analysis time was 57 min. A total of 1 µ of the diluted sample (1/100 in petroleum ether, v/v) was injected in the split mode (ratio 1:10). The injector was operated at 200 • C. Quantitative data were obtained electronically from the FID area percent data without the use of correction factors. Peak integration and quantification were performed automatically with Saturn 2100 Workstation software. A check of the integration of each peak was carried out and corrected manually if necessary.
Essential oil analysis was also performed with a GC chromatograph equipped with a Varian Saturn GC/MS/MS 4D mass selective detector in the electron impact mode (70 eV). MS was adjusted for an emission current of 10 µA and an electron multiplier voltage between 1400 and 1500 V. The trap temperature was 150 • C, and that of the transfer line was 170 • C. Mass scanning was from 40 to 650 amu. The components were identified based on the comparison of their Kovats indices (KI), co-injection of standards, and MS experimental data, with those contained in commercial or literature libraries (NIST 02 version 2.62, Adams, 2001) [22]. Alkanes (C5-C24) were used as reference points in the calculation of KI. GC and GC-MS analysis results are given in Table 2. All the determinations were performed in duplicate and averaged.

Extract Preparation
A total of 2 g of leaf powder was crushed and extracted with 20 mL of methanol for 24 h at ambient temperature and in darkness. After filtration and removal of methanol under reduced pressure in a rotary evaporator, the dry residue was dissolved in absolute methanol (1 g of extract in 10 mL of methanol) for conservation and analysis.

Quantity Composition of Polyphenol
The dosage of total polyphenols was determined with Folin-Ciocalteu reagent using the method of Mau et al. (2001) [23]. The absorbance measured at λ = 760 nm with a UV-visible spectrophotometer is proportional to the quantity of polyphenol present in the plant extracts. A total of 1 mL of the diluted samples was mixed with 1 mL of Folin reagent and 4 mL of ultra-pure water. After incubation for 3 min at ambient temperature, 10 mL of sodium carbonate solution, Na 2 CO 3 (1 M), was added. This mixture was adjusted to a volume of 20 mL by the ultra-pure water. The mixtures were incubated for 90 min. To ensure the reliability of the results, the dosage of each phenolic compound was realized in three tests.
The phenol standard used is the Gallic acid. The levels of total polyphenols were expressed as the milligram gallic acid equivalents (GAE) per gram of dry weight (mg GAE/g DW).

Total Flavonoids Content
The determination of flavonoids concentration was carried out by Djeridane et al. (2006) [24]. The method is based on the formation of a complex aluminum-flavonoid with a yellow color in the presence of the soda. This staining absorbs in the visible range at 430 nm. A total of 1 mL of each appropriately diluted extract or standard solution of rutin was mixed with 1 mL of aluminum trichloride solution, AlCl 3 (0.3 M); the mixtures were incubated for 15 min. The concentrations of flavonoids were expressed as the milligram rutin equivalent per gram of dry weight (mg RE/g DW). Samples were analyzed in triplicate.

HPLC Analysis of Phenolic Profile
The HPLC system consisted of a RP-HPLC (serie 1200, AGILENT TECHNOLOGIES) series instrument, coupled to a quaternary pump, a diode array detector (DAD), an autosampler and a column compartment. UV-visible spectra were recorded from 210 to 520 nm and chromatograms were monitored at 280 nm, 320 nm, and 360 nm. Samples were separated on a Spherisorb C18 (5 m, 250 × 4.6 mm) column, with a sample injection volume of 0.01 mL. The mobile phase consisted of water-formic acid (100:0.1, v/v) (A) and MeOH-formic acid (100:0.1, v/v) (B). The eluting conditions applied were: 0-5 min, linear gradient from 10% to 20% B; 5-15 min, linear gradient from 20% to 40% B; 15-45 min, linear gradient from 40% to 100% B, for washing return to 10% B at 50 min, and finally 5 min isocratic to reequilibrate the column. The mobile phase flow rate was 0.7 mL/min and the column temperature was controlled at 30 • C [24]. Table 2. Chemical composition (% of the total essential oil) of essential oil from leaves of the eight M. vulgare L. populations.

Statistical Methods
The results were presented as the means ± SEM of the triplicate measurements. To better compare the means of the characters between populations, we conducted oneway analysis of variance (ANOVA). The means of the populations were the mean of the overall observations, allowing for defining the gap between the different populations. A comparison of the means by the Duncun test was also performed (SAS software).
To better estimate the divergence between the populations based on the chemical markers, we conducted principal components analysis (PCA) (SAS, 2006) [25]. This was based on terpenes, phenols, and flavonoids content, and all parameters conjoined. Correlation analysis between all the parameters was also carried out using the statistical correlation test (SAS, 2006).

Qualitative Analysis of Essential Oil Composition and Population Structure
The components of the essential oils were identified by their percentage and their retention indices (RI). Table 2 presents the components with a percentage above 0.1 in order of their elution on the HP-5 column. The components that are present in a percentage lower than 0.1, or as trace, are not listed. The composition of the oil varies between the different locations. Analysis of M. vulgare essential oil revealed the presence of 42 compounds which represents 96.08-100% of the total oil ( Table 2). The hydrocarbon sesquiterpenes are the main fraction in all the populations tested, and the highest percentage was recorded in the Zagouan population. β-bisabolene is the major constituent, with levels ranging from 32% in population Beja (1) to 65.2% in population Zagouan (5).
Furthermore, β-bisabolene has a balsamic odor and is approved in Europe as a food additive. βand γ-bisabolenes have been found to possess anticancer properties [26]. The present study revealed that the population of Kasserine had benzodioxole (phenols) as their major component (50.14%). The second major compound was germacrene D (sesquiterpene hydrocarbons), with levels varying from trace in P2, P5, and P7, to 17.05% in population Nabeul (3). Terpinen, camphor, borneol, and α-terpineol were present only in the population Beja (1). The β-caryophyllene (sesquiterpene hydrocarbons) was frequently shared between different populations except population 7. On the other hand, eudesmol and isocaryophyllene were found only in population Gabes (8), and benzodioxole and trans-isodillapiole (phenol) were present only in the population of Kasserine (7) ( Table 2).
Importantly, sesquiterpene hydrocarbons represent the main fraction of the oil, this was in line with the chemical composition of M. vulgare grown in Ouled Mnasser Djebeniana, Tunisia [20]. It is interesting to note that the presence of benzodioxole (a phenylpropanoids derivative) in population 7 (Kasserine), as a major compound with 50.14% of the total oil, has never been found in the essential oils of the Marrubium genre or Lamiaceae family. A similar result was elucidated in the young roots and aerial parts of Astrodaucus persicus (Apiaceae) extract [27]. Benzodioxoles are important in health and used as antitumor, antibacterial, antifungal, antiparasitic, antimalaria, and antioxidant drugs, as well as in pesticides and herbicides [28]. This population also contains a high proportion of trans-isodillapiole (19.07%) (a phenylpropanoids derivative). This specific composition could probably be due to an adaptation of M. vulgare to the bioclimatic conditions of the region or to intrinsic factors related to the genetics of the plant. Kadri et al. (2011) [7] also found that the major compound of M. vulgare essential oil is eudesmol. However, this compound was only detected in the essential oil of population Beja (5). The more abundant chemical component in Boussalem and Tunis is eugenol [29]. Furthermore, the citronellol and lemonellyl format was not detected in all the extracts. β-bisabolene is a major component of the essential oil of M. vulgare from Iran [30]. Khanalvi et al. (2005) [31] have found that the major constituent of M. vulgare from Turkey is β-bisabolene. Although Asadipour et al. (2005) [32] have revealed that caryophyllene oxide, caryophyllene, and germacrene D are the main fractions. The main compound of M. vulgare oil from Poland was caryophyllene [33]. Nagy and Svajdlenka (1998) [34] revealed that the major constituents of M. vulgare essential oil from the Czech Republic are caryophyllene and germacrene D. However, Weel et al. (1990) [35] have identified Z-Farnesene, caryophyllene, (E)-2 hexanal, and α-humulene, as new chemotypes of Lithuanian M. vulgare. A specific composition with the presence of chemical components that are not identified as the usual constituents of M. vulgare essential oil in other parts of the world (significant percentages of the phenolic compounds, fatty acids, and alkanes) is revealed. A quantitative and qualitative variation concerns both minor and major compounds, indicating that the environmental factors strongly influence its chemical composition ( Table 2).
The chemical structure of the populations by principal components analysis (PCA) performed on the terpene markers, most present and shared between populations, shows that the first two PCA axes represent 78% of the total variation ( Figure 1). The populations were clearly isolated and subdivided into four distinct groups (Figure 1), but this separation was not based on their geographical and/or bioclimatic origins. Accordingly, the observed wide variation could not only be explained by climate variation because there were both quantitative and qualitative variations of terpenic compounds within the same ecological group, and between the geographical locations of the populations. Some authors report that high chemical divergence was not correlated with geographic distance with regard to the structuration of many other medicinal and aromatic plants [36,37]. Significant differences in the chemical compounds of the essential oils have been found between geographically near populations (Nabeul, Sousse, and Zagouan or Kef and Kasserine). Additionally, this observed terpenic variability between M. vulgare populations can be explained in part by the variation of environment parameters that, defined by Emberger (1966) [21], include annual rainfall and the difference between the maximum temperatures of the warmest months and the minimum temperatures of the coldest months. Although various other environmental factors (topography, altitude, seasonal distribution of precipitation, etc.) would have a great deal to do with the variation of the chemical compounds in essential oil [38][39][40]. Additionally, chemical variability could be explained by local abiotic factors characteristic of the scaling zone and/or selective biotic factors (fauna and flora) [37]. Edaphic conditions also play an important role in the modification levels of these compounds [4]. It should also be noted that the geographical area of all Mediterranean countries, including Tunisia, is characterized by variable reliefs including long mountain ranges relatively close to the sea. As a result, climatic differences exist between the neigh-boring regions, mainly due to differences in altitude reflected in the differentiation of local bioclimate [37,38]. However, the variation in terpene levels between populations may also result from the influence of plant-specific genetic factors, that are related to the plant's reproductive patterns [41,42]. Additionally, the effect of genotype-middle interaction can play an important role in this variation. Considering this conclusion, it is worth noting that there are significant qualitative and quantitative variances in essential oils among populations, implying that environmental influences have a significant impact on their chemical composition.

Analysis of Total Polyphenol
Polyphenolic substances are the key chemical groups that have the potential to remove radical species, inhibiting the development of oxidative chain reactions, and functioning as primary antioxidants or free radical terminators [43], hence, quantifying polyphenols in various samples is critical. Plants' phenolic composition and content vary widely due to genetics and environmental factors [44]. Numerous studies [8,45,46] have shown that intraspecific diversity amongst populations from various locations can lead to differences in antioxidant properties. Our results showed that the quantification of secondary metabolites from Marrubium vulgare, which represents a natural antioxidants source, indicated that the highest amount of phenolic compound was found in the methanolic extract of the Kasserine and Kef populations (44.65 and 42.16 mg GAE/g DW, respectively). However, the lowest amount was found in Nabeul's methanolic extract, with a content of 20.80 mg of GAE/g DW ( Table 3). Analysis of the variance to one criteria of classification (population effect) on the average of the total polyphenol levels shows highly significant variations between populations (p > 0.01) ( Table 3). This significant variation between populations may be due to the variation in environmental conditions (climate, pressure, humidity and edaphic) [47,48], while the polyphenols are involved in the adaptation of species to the bioecological conditions [49,50]. The Duncun test is used to separate the population into three groups, i.e., populations 7, 6 and 1 form the first group, while populations 8, 2 and 5 compose the second group. However, populations 4 and 3 are agglomerated in a third group (Figure 1). Our result is more important than the levels detected in the M. vulgare from other origins in Tunisia [29], and it is different from those obtained from M. vulgare in Poland [51]. However, this agglomeration does not conform with the bioclimatic characteristics of the populations (Figure 2). In fact, populations from inferior humid bioclimates (Beja and Bizerte) have very different total phenolic contents, and rearranging them into different groups with Duncun test analysis, indicated that edaphic conditions are one of the major factors influencing the differences in the chemical composition of metabolites and bioactivities in plants. Other researchers in Tunisia species demonstrate substantial differences in the total polyphenols content and explained this variability by edaphic characteristics, especially soil salinity [52]. Table 3. Total polyphenol and flavonoid levels in leaves of the eight M. vulgare L. populations. The results are expressed in mg of gallic acid equivalent/g of dry weight (mg GAE/g DW) for polyphenols and mg rutin equivalent/g of dry weight for flavonoids.

Individual Phenolic Acids Compounds in M. vulgare
M. vulgare is an abundant source of various phenolic compounds, major classes being phenolics acids and flavonoids. The composition of the identified compounds presented eight compounds in eight populations. We identified ferulic, quercetin, p-coumaric and caffeic acids as the main detected flavonoid and phenolic acids (Table 4). Our results are in agreement with those reported for Polish M. vulgare, where ferulic acid (36.2 mg/100 g dry matter), luteolin (616 mg/100 g dry matter) and apigenin (43.8 mg/100 g dry matter) were the major phenolic compounds [12]. In the present study ferulic acid is the main phenolic compound in all the populations, except Kef, where the highest amount was recorded in catechin, which occurs as the main constituent of M. vulgare. It is worth noting that phenolic acid yields depend on the leaf sampling locality. Indeed, the best yield of ferulic acid was observed for the Bizert extracts, followed by those of Beja and Kasserine, respectively. These changes can be explained by the climatic and edaphic differences characterizing each geographical region. The antioxidant properties of phenolics are associated with phenolic hydroxyl groups connected with ring structures [30]. Interestingly, the antioxidative properties of M. vulgare were associated with hepatoprotective [31] and anticancer activity [32].

Analysis of Flavonoids Composition
Flavonoids are the main antioxidant components; they are a class of polyphenols [52]. The highest level of flavonoids was revealed in the methanolic extract of Kef (37.48 mg RE/g DW) (Table 3). However, the lowest amount was found in Nabeul's methanolic extract, with a content of 8.91 mg RE/g DW. Our results confirm the findings by Amri et al. (2017) [53], which revealed a high content of phenols and flavonoids with colorimetric assays, in methanolic extracts of M. vulgare in Tunisia. In the literature, M. vulgare is reported to be rich in flavonoids [54]. However, the values detected for other populations are relatively higher (Bizert, Beja, Zagouan, Kef, Kasserine and Gabes) ( Table 3). Analysis of the variance to one criteria of classification (population effect) on the average of the flavonoid levels shows highly significant variations for all the tested populations (p > 0.01). For the Duncun test there are three groups of populations: population 1, 6 and 7 form the first group; 2 and 5 compose the second group, however, populations 3, 4 and 8 are regrouped together in a third group (Figure 3).

Structure of Population Based on Polyphenols and Flavonoids Content
The principal components analysis (PCA) performed on the total polyphenol and flavonoids levels established according to the first two principal components (100% of the total variation, Figure 3), revealed that population groupings in three agglomerations, not pertaining to their bioclimate. Climate variation alone was unable to explain the chemical heterogeneity between the populations.
A correlation test between polyphenols and flavonoids levels shows that there is a positive and highly significant correlation (r = 0.853, n = 24, p < 0.0001). Populations rich in total polyphenols generally have the highest levels of flavonoids, including the population of Kasserine, Kef, and Beja. (Table 3). Moreover, the populations with low concentrations of polyphenols also have low levels of flavonoids: Sousse and Nabeul. This agglomeration confirmed that obtained with the Duncun test.

PCA of Combined Analysis
PCA is a multivariate statistical analysis that can be used to examine and simplify complex and huge datasets (Figures 4 and 5). The pattern of variation in M. vulgare was also analyzed using principal components analysis (PCA) to evaluate the species and their link with the observed traits, based on the correlation between the traits and the extracted clusters. The chemical profiles of plants that species were grouped and clearly separated from the populations. The joint analysis by principal components analysis (PCA), based on phenolic and terpenic markers combined, showed that the chemical composition of the populations was influenced by these two quantitative markers. Indeed, there was no change in the profile of the terpene compounds of populations 1, 5, 6 and 8, and that was explained by the fact that populations 1 and 6 both have high levels of terpene compounds (most correlated to PC 1) and high total polyphenols. Population 5 has both higher and lower levels of phenolic compounds, and high levels of total polyphenols. Population 8 has moderate levels of terpene compounds and phenolic compounds. Compared with PCA only performed for phenolic compounds, populations 3 and 4 showed different locations, according to their low phenolic content; these populations also had average levels of terpenes. The population of Kasserine (7) showed that the impact of climate variation alone could not explain this chemical variability. It can, therefore, be concluded that the quantitative determinism of the genes encoding these adaptive traits (the secondary metabolites) was complex and was controlled by various environmental and plant genetics factors.  All the results on the chemical structuring of populations show that chemical polymorphism between natural populations of M. vulgare in Tunisia was very important, regardless of the used marker. They also show that the determinism of quantitative genes encoding these adaptive traits was influenced by several factors. This complexity was due to the importance of the plant genotype effect, which was the first assuring biosynthesis of these biomolecules, and to the importance of the effect of genotype-middle interaction, that regulates the expression of genes encoding these secondary metabolites, while also noteworthy is the major effect exerted by the environment, such as the climate, soil texture, pathogens, etc.

Conclusions
M. vulgare from Tunisia is a chemotype of β-bisabolene. A significant quantitative and qualitative variation in the chemical compounds of the essential oils is detected. This chemical divergence concerns both major and minor compounds. M vulgare populations are very rich in total polyphenols and flavonoids. The chemical structuring of populations by PCA, based on the most present and shared terpene markers, and the total polyphenol and flavonoids levels separately, is not necessarily explained by the populations' bioclimatic or geographic characteristics. This also reflects that climate variation could not explain the observed wide chemical variation. Moreover, the joint action of all the factors (terpenes, polyphenols, and flavonoids) defines the quantitative genetic diversity. The latter seems to be very abundant in the populations of M. vulgare in Tunisia. This quantitative genetic diversity, probably controlled by polymorphic genes, is an adaptation strategy to the changes in the environmental conditions. Overall, this form of genetic diversity plays a very important role as a solution to the problems that threaten the species.