The Role of Biostimulants as Alleviators of Biotic and Abiotic Stresses in Grapevine: A Review

The viticulture and wine industry contribute to the economy and reputation of many countries all over the world. With the predicted climate change, a negative impact on grapevine physiology, growth, production, and quality of berries is expected. On the other hand, the impact of these changes in phytopathogenic fungi development, survival rates, and host susceptibility is unpredictable. Grapevine fungal diseases control has been a great challenge to winegrowers worldwide. The use of chemicals in viticulture is high, which can result in the development of pathogen resistance, increasingly raising concerns regarding residues in wine and effects on human and environmental health. Promoting sustainable patterns of production is one of the overarching objectives and essential requirements for sustainable development. Alternative holistic approaches, such as those making use of biostimulants, are emerging in order to reduce the consequences of biotic and abiotic stresses in the grapevine, namely preventing grape fungal diseases, improving grapevine resistance to water stress, and increasing yield and berry quality.


Introduction
Grapevine (Vitis vinifera L.), a perennial woody plant, constitutes the most valued fruit species globally and has been linked to agricultural and religious activities [1,2]. Most parts of the grapevine are used, principally berries, for manufacturing various industrial products, such as, wine, raisins, pressed juice, and spirits [3][4][5]. The winemaking sector has a socio-economic relevance in many countries, contributing to the exports and sustaining many wine-related activities, including tourism. In 2020, five countries represented 50% of the world vineyard surface area, namely: Spain (13.1%), France (10.9%), China (10.7%), Italy (9.8%), and Turkey (5.9%) [6]. According to OIV, in the same year the European countries were the biggest wine producers, namely, Italy (49.1 mhl), France (46.6 mhl) and Spain (40.7 mhl), followed by the USA (22.8 mhl) and Argentina (10.8 mhl) [6]. It is known that biotic and abiotic stresses can limit the growth and also the yield of plants [7]. In the global climate change scenario, viticulture faces new challenges and threats. The winegrowing regions are restricted at geographic and climatic level, which is synonymous of quality and optimum production [8]. These regions present a specific "terroir", which includes specific soil, topography, climate, landscape characteristics and biodiversity features, and interaction with applied vitivinicultural practices [9,10]. The climate change impacts in and enzyme inactivation, and reduce flavor development in the fruit; and (iv) during the maturation stage, a pronounced diurnal temperature range enhances the synthesis of tannins, sugars, and flavors; the grape acidity can be reduced, because the malic acid content decreases with high temperatures [22,24,28].
On the other hand, a grapevine is susceptible to various pathogens, fungal diseases being the major risk that compromises their cultivation and economic profit worldwide [29,30]. The most problematic fungi and fungi-like pathogens to Vitis vinifera are: downy mildew (Plasmopara viticola), powdery mildew (Erysiphe necator) and gray mold (Botrytis cinerea) [31][32][33]. These infections reduce fruit quality and yield, either by direct infection of berries themselves or through a decrease in plant vigor [33]. Many synthetic chemical pesticides are used intensively and became indispensable for traditional agriculture, preventing, and limiting pathogen infections. It is known that viticulture is one of the most treated cropping systems in the world; in wine-growing regions fungicides can account for more than 90% of all pesticide applications [29,30]. To control downy mildew the winegrowers frequently use products with copper, namely in organic production [34]. Due to the continuing use of copper and the fact that it can induce phytotoxicity in the grapevines and lead to an accumulation of this heavy metal in vineyard topsoil, the European Commission has taken some restrictive measures in the use of copper-based formulations. Moreover, the use of the other fungicides is limited. The restrictions applied by the European Commission are on the number of pesticide treatments (Directive 2009/128/EC) and on the maximum quantity of copper fungicides per year (Regulation 2002/473/EC). Copper has been added to the list of candidates for substitution (European Commission Implementing Regulation 2018/84) and, since February 2019, further limited to 4 kg per hectare/year spread over 7 years (European Commission Implementing Regulation 2018/1981 of 13 December 2018) [35]. In parallel, there is a demand by consumers for more environment-friendly products. Consequently, research in innovative bio-strategies is necessary. Recently, a study contributed with an innovative approach to obtain specific protection against the causal agents of grape downy mildew (P. viticola) [36]. In this study, the authors used the yeast two-hybrid approach and the P. viticola cellulose synthase 2 (PvCesA2) as target enzyme to identify interacting peptides, potentially capable of inhibiting this enzyme. Thus, they demonstrated that the peptide NoPv1 prevents P. viticola germ tube formation and grapevine leaf infection without affecting the growth of non-target organisms and without being toxic to human cells. This approach may bring many benefits in the future due to its specificity and because P. viticola is one of the biggest problems for viticulture.

Use of Biostimulants as a Mitigation Strategy for Biotic and Abiotic Stresses in Grapevine
A biostimulant is a formulated product containing mixtures of natural substances and/or microorganisms applied to plants with the aim to enhance nutrition efficiency, abiotic and biotic stress tolerance, and/or crop yield and quality traits, regardless of its nutrients content, plant growth regulators, or plant protective compounds [20]. In general, nine categories of substances that act as biostimulants can be defined: (i) humic substances; (ii) complex organic materials (obtained from agro-industrial and urban waste products, sewage sludge extracts, composts, and manure); (iii) beneficial chemical elements (Al, Co, Na, Se, and Si), (iv) inorganic salts including phosphite; (v) seaweed extracts (brown, red, and green macroalgae); (vi) chitin and chitosan derivates; (vii) antitranspirants (kaolin and polyacrylamide); (viii) free amino acids and N-containing substances (peptides, polyamines, and betaines); and (ix) plant growth-promoting rhizobacteria (PGPR), arbuscular mycorrhizal fungi (AMF) and Trichoderma spp. [37]. Due to the complexity of the extracts and the wide range of molecules contained in the solutions, it is very difficult to understand which are the active compounds [38]. Biostimulants are usually able to improve vigor, stimulate vegetative growth, improve nutrient uptake and distribution within the plant, increase the antioxidant capacity of plant tissues and enhance tolerance to biotic and abiotic stress, consequently improving plant yield and fruit quality [27,39]. These products contain some bioactive molecules called elicitors, which have a beneficial effect on plants and improve their ability to face adverse environmental conditions, acting on primary or secondary metabolism [18]. The term "elicitor" refers to all the signal molecules that are perceived and that induce a defensive reaction in the plant [16]. It has been reported that exogenous application of elicitors, can induce the activation of enzymes involved in the synthesis of phenolic compounds and, consequently, can play a key role in plant-pathogen interactions [18,29]. In the case of grapevine, diseases usually take an excessive application of fungicides that have several negative impacts, such as development of fungicide resistance; accumulation of fungicide compounds in the vineyards topsoil; ecological consequences on soil, water, fauna, and risks to human health [34,40,41]. In addition, pesticide residues have been identified in the wine, affecting the natural yeast communities necessary for winemaking, as well as its aroma [29]. With the need to preserve the quality of wine and to reduce the impact of pesticides on the environment and human health, considerable interest has been focused on replacing chemicals with innovative bio-strategies, such as environment-friendly products, to promote sustainable agriculture and food production systems. Thus, the use of biostimulants as biofungicides is a promising alternative for the control and prevention of fungal and fungal-like diseases. They are currently considered an emerging class of crop management products that aim to moderate crop stress in order to increase crops productivity. Over the last decade, utilization of natural plant biostimulants is gaining importance, and these plant extracts have been analyzed in several studies in different crops, in order to reduce the use of chemicals [42][43][44][45][46]. The use of plant extracts as biostimulants gives importance to plants that are normally undervalued, without any commercial interest. Thus, these plants become useful to improve the performance of plants and at the same time to control fungal diseases in grapevine and other plant species.

Biostimulants Used in Grapevine
Winegrowers have the need to preserve the quality of the grapes and consequently of the wine and face a great challenge due to climate change that increases biotic and abiotic stress. At the same time, it is necessary to make viticulture more sustainable and environment-friendly, reducing the use of chemical products. There are several innovative bio-strategies already used, as is the case with biostimulants. Some of the most frequently used in the vineyard are shown in Figure 1. The different biostimulants were grouped according to the stress they fight, biotic, abiotic, or both and those that act as elicitors. Some of these biostimulants are plant extracts, namely nettle, Japanese Knotweed, and seaweed extracts. There are other products, such as yeast extracts, urea, kaolin, and others, which are also beginning to be interesting in combating stress in the vine caused mainly by climate changes.

Biostimulants as Abiotic Stress Alleviators Kaolin
Kaolin is a chemically inert and non-toxic clay that can reflect radiation [47]. This compound is widely used as a mitigation strategy to handle summer stress, namely water stress, excessive radiation that is absorbed by leaves and grape berry clusters, elevated air temperature, and high evaporative demand [2,10]. Foliar application of this clay mineral has become commonly used in Portuguese vineyards and is very common in Douro Demarcated Region (DDR) [2,47,48]. Some studies report some concerns about this compound because it forms a particle film that can affect the photosynthetic rate [2], while other studies proved otherwise the stimulation of the photosynthetic activity (Table 1) [47]. Indeed, the study of Dinis et al. [47] proved that the reflective film caused by kaolin application can protect the photosystem II structure. This study highlights the impact of kaolin application on the photosynthetic activity of grapevine in different growing conditions (light and irrigation), which provides new insights for the application of this compound towards the adaption of the grapevines to different climate conditions.

Seaweed Extract
Grape/wine A. nodosum extracts, improved quality, increased plant defenses by the regulation of molecular, physiological and biochemical processes.
[ [53][54][55][56][57] Grapevine Improved rooting of the cuttings, increased the number of new shoots, their length and number of internodes, as well as the chlorophyll content in the leaves. [58] Grape/wine The volatile profile (raising the levels of total acetals and alcohols) in cv. Groppello Gentile, responsible for the wine flavor and taste. No substantial effect on phenolic content including anthocyanins, stilbenes, and flavonols, on either grape or wine of cv. Tempranillo. [17,59] Yeast Extract Grape/wine Saccharomyces cerevisiae extracts enhanced anthocyanin and stilbene contents in grape and wine of cv. Tempranillo. [17] Grape A mixture of Laminaria digitata and Saccharomyces spp. Extracts used to control P. viticola in the cv. Montepulciano did not affect grape quality (amino acid content). [34]

Treatment Matrix Effect References
Japanese Knotweed (anthraquinone) Grape Protect against P. viticola in cv. Chasselas. [42] Kaolin Grape Enhanced photosynthetic activity, improved antioxidant capacity, increased concentration of phenolics including anthocyanins, vitamin C and sugars, in addition to protecting the leaves and berries of cv. Touriga Nacional from solar radiation. Protected the berry exocarp from light stress in cv. Alvarinho. [2,10,47,48] Glycine Betaine Grapevine Increased the resistance to water stress. Significantly affected leaf relative water content (RWC), canopy temperature, chlorophyll content, soluble carbohydrate contents, and leaf area. Under drought stress, seemed to influence the leaf water status, slightly alleviating the leaf water loss and increasing leaves fresh weight in cv. Alphonse Lavallée. [60,61] Methyl Jasmonate Grapevine Enhanced tolerance of grapevine foliar cuttings and vineyard against the pathogen E. necator in cv. Cabernet Sauvignon. [33] Grape/wine Increased the phenolic compounds content, depending on the variety and vintage. [17,[62][63][64] Abscisic Acid Grape Increased • Brix, total phenolic and anthocyanin contents and improved the production of volatile aromas in cv. Cabernet Sauvignon berries. [65] Salicylic Acid Grape Increased total phenolic content and antioxidant activity in cv. Syrah. Delayed berries ripening in the cvs. Bez El Naka and Shiraz [66][67][68] Others Grape Urea: Increased yeast assimilable nitrogen, berry amino acid, flavonoid, stilbene and anthocyanin concentrations. Foliar N and foliar N + S: Improved vine nitrogen status and enhanced aroma expression in Sauvignon blanc wines without the negative impact on vigour and Botrytis susceptibility. Phenylalanine: Small improvement in some phenolic compounds.

Nettle
Nettle (Urtica dioica) is an herbaceous perennial flowering plant that belongs to the Urticaceae family and Urtica genus and is used in a large diversity of agronomic crops, as fertilizer, as forage, or as livestock feed [72]. It is native to Eurasia and occurs as a perennial plant in temperate zones of Asia, America, and Europe [72,73]. Various parts of the nettle have been used for human welfare; it is historically used as a medicinal plant and has great economic potential due to its multi-utilitarian nature [72,74]. It is popularly cooked green in many regions due to its high protein content, 21-23% crude protein, and 9-21% crude fiber [73]. Nettle is easily digestible and has a high content of minerals and vitamins, especially iron, manganese, potassium, calcium, vitamin C, D, and pro-vitamin A [72,73,75]. The two most prevalent active chemical agents found in nettle are formic acid and histamine, which function as anti-inflammatory agents [73]. In biodynamic viticulture, an alternative agricultural technique, nettle is widely used in biodynamic preparations [76][77][78][79]. Some studies also show that nettle is efficient as an antifungal against different genera and species of fungi, namely Alternaria alternata and Rhizoctonia solani [74]; Curvularia lunata, Alternaria solani, Alternaria zinniae, Fusarium oxysporum [80]; Phytophthora spp. [43] and Botrytis cinerea [46]. In the future, more studies are needed on the use of nettle extracts on the grapevine, as these extracts seem promising in combating and preventing fungal diseases.

Japanese Knotweed
Japanese knotweed (Fallopia japonica) is an invasive plant native from Asia, namely, Korea, China, and Taiwan [81][82][83]. This is a woody-stemmed herbaceous perennial rhizomatous plant, member of the buckwheat (Polygonaceae) family and has different scientific names, Reynoutria japonica, Polygonum cuspidatum, and the most used Fallopia japonica [82,84,85]. Fallopia japonica was introduced in Europe and North America more than 100 years ago as a source of food and as an ornamental plant [85][86][87]. Japanese knotweed is able to produce high concentrations of secondary metabolites (stilbenes, tannins, lignin, anthocyanins, sterols, phenethyl alcohols, and essential oils), anthraquinones being the most important [83,85]. At the agronomical level, anthraquinones have a lot of applications: as a repellent from pests, namely to combat mosquitos [88]; in sunflowers, to protect the seeds against blackbirds [89]; and as an antifungal in grapevine [45] (Table 1).

Biostimulants as Abiotic and Biotic Stress Alleviators
The use of seaweed formulations as biostimulants has been reported for many years and is well established [90,91]. Macroalgae form an integral part of marine coastal ecosystems. It has been estimated that there are about 9000 macroalgae species [92]. They are classified into three main groups based on their pigmentation: brown (Phaeophyta), red (Rhodophyta), and green algae (Chlorophyta) [92]. Many seaweed species are an underutilized bioresource, often used as a source of food, industrial raw materials, and in therapeutic and botanical applications, in agriculture and horticulture to feed livestock, for soil fertilization, and in the form of extracts to promote plant growth [90,92]. Seaweeds contain several macro and micronutrients, vitamins, amino acids, cytokinins, auxins, and abscisic acid [91,92]. At the agronomical level, a number of commercial seaweed extract products are available, and several studies have shown their good results [92]. Brown seaweeds are the most used type in agriculture, Ascophyllum nodosum L. being the most studied [49,50,[93][94][95][96][97][98][99][100]. Some studies in Vitis vinifera have shown that foliar application is beneficial to control and prevent the impacts of biotic and abiotic stress (see Table 1).

Chitosan
Chitosan has antimicrobial properties and is able to elicit plant defense to pathogens [17]. This compound is used in grapevine to control fungal diseases [34,[53][54][55]. Chitosan is a beta-1,4-linked glucosamine, a deacetylated derivative of chitin, present in the shells of crustaceans, in insects and in certain organisms, such as fungi, algae, and yeast [29,53,58]. It is a polymer that forms a semipermeable film around plant tissues, inhibiting several pathogens and induces defense response mechanisms in the host tissues [34]. Several studies demonstrate the use of this compound in the vineyard and the capacity to improve the grapevine defense responses and also the grape production and quality under stress ( Table 1). The effectiveness of treatment depends on its concentration and, the stage of plant development at a time when they were exposed to the drought or heat stress [58]. In the future, the use of chitosan as a biostimulant, may be useful to prevent and control some of the most important fungal-like diseases in grapevine, with the benefits for the consumers that are concerned about the presence of fungicide residues in berries and wines. This biostimulant is a good candidate for the substitution of products with copper frequently use by winegrowers, since it is a natural compound, safe for humans and the environment, its use is a strategy welcomed by organic growers and can be applied to manage grapevine diseases [55].
Yeast extracts have been used in grapevine with the aims such as improving the synthesis of phenolic amino acids and, volatile compounds in berries and, subsequently, in the wines [18]. Several studies using yeast extracts compare its effectiveness to that of other compounds, such as methyl jasmonate, chitosan, and seaweeds [17,34,62,104,105]. Yeast extracts (Saccharomyces cerevisiae) have been used in cv. Tempranillo retrieving better results when compared with methyl jasmonate and chitosan in terms of berry and wine anthocyanin content [17]. Several authors reported different inter-annual and inter-varietal responses in grapevine to the foliar applications of yeast extracts. In a study using yeast cell wall, as preharvest treatments, in the cvs. Monastrell and Tempranillo, annual and varietal differences in stilbene content were detected [62]; in another study inter-varietal differences in the amino-acid contents were detected in the cvs. Garnacha, Graciano and Tempranillo sprayed with yeast extracts [104]. On the other hand, the same authors reported that the application of yeast extracts has a physiological cost for the vines, leading to a decrease in the content of free and total amino acids in the cv. Tempranillo, although inducing resistance to diseases. Other studies with these extracts in grapes and wines are presented in Table 1. Yeast extract applications in grapevine have many benefits, but the consequences in physiological parameters of the plant must be considered. Considering this, in the future it may be a simple practice to increase the phenolic content of grapes and wine and to combat and prevent fungal diseases. However, further studies are needed to determine which cultivars benefit the most from using yeast extracts as biostimulants in the vineyards.

Methyl Jasmonate
Methyl jasmonate (MeJA) is a derivative of jasmonic acid (JA), known to be a ubiquitous plant signaling compound and was primarily isolated from the essential oil of Jasminum grandiflorum [65]. Methyl jasmonate is an endogenous plant regulator, that acts as a signaling molecule upon plant stress and can be involved in mechanisms of plant defense by the synthesis of secondary compounds [17]. MeJA is one the most used elicitors on grapevine, that can induce defense mechanisms [18]. Increase of transcript levels coding pathogenesis-related proteins (PR proteins), coding enzymes involved in phytoalexin biosynthesis, have been verified and correlated with the accumulation of stilbenes (antimicrobial compounds) [33]. This study confirmed the eliciting activity of MeJA. Several studies also report the exogenous application of MeJa as inducer of berry and wine quality ( Table 1). Application of this compound may be a simple and innovative strategy to improve the physicochemical and physiological parameters of grapevine, increasing berries and wine quality. In the future more studies are needed to prove the elicitor efficiency of methyl jasmonate against grapevine pathogens.

Abscisic Acid
Abscisic acid (ABA) is an elicitor extracted from plants and is used in grapevines as a growth regulator [18,63,65]. ABA protects plants from environmental stress and has a positive regulatory effect on plant growth [65,106]. ABA can induce responses to water, light, and thermal stresses [106]. ABA can be involved in the transformation of sugar and organic acids in berries (Table 1) [65] and can also enhance the expression of key genes (Phenylalanine Ammonia Lyase (PAL), Chalcone Isomerase (CHI), and Myeloblastosis transcription factors (MYB)) in the synthesis pathway of polyphenols and anthocyanins. This hormone can be used as a biostimulant to improve grape and wine quality.

Salicylic Acid
Salicylic acid (SA) plays an important role in plant development, enhances plant vigor under biotic and abiotic stresses, and is one of the endogenous signals involved in mediated responses associated with resistance to biotrophic pathogens [14,107,108]. Several studies have shown that the exogenous application of SA enhances the growth and productivity of plants [108]. In cherries, it was used to prevent cracking [98]; in olive trees-to protect from drought [109]; and in strawberries-to improve fruit quality [110]. In the case of grapevine (Table 1), SA has been demonstrated as an important secondary metabolite playing an essential role in determining berry quality parameters such as color, flavor, astringency, and bitterness [66]. Despite all the advantages that this compound seems to present, it is known that exogenously applied SA may have a negative effect on photosynthetic activity [15]. Like most other classes of plant hormones, the application of SA at levels above the ideal can cause inhibitory effects on the growth, physiological or metabolic processes of plant tissues; at lower levels (close to the ideal) it can often have beneficial effects on these processes, especially in stressed plants [15]. In the future, the exogenous application of SA to vineyards would be an interesting agronomic practice for obtaining grapes with improved properties. However, the effect may depend on the combination of several factors, including genotype, type/level of stress and the concentration applied.

Glycine Betaine
Glycine betaine (GB) is an N-trimethyl glycine derivative compound that belongs to the class of quaternary amines and can be found in a wide range of bacterial, plant, and animal species [111,112]. GB has several properties, attractive for use in stress protection, since it is naturally synthesized, readily available, non-toxic, inexpensive, and maintains water content in plant cells by lowering osmotic potential in osmotic adjustment [111,[113][114][115]. This is a convenient compound for the induction of crop tolerance to various abiotic stressors, such as frost, extreme temperatures, or drought [112,113,116]. There is evidence suggesting that GB plays a role in response to stresses in some plant species, where the accumulation occurs mainly in chloroplasts to protect the thylakoid membrane [113]. This product retains high photosynthetic capacity, promoted plant growth, maintained the yield, and enhanced performance under stress [15]. The exogenous application of GB was used in different crops, such as sweet potato [117], alfalfa and cowpea [118], strawberry [112], and cherry [119]. The application of glycine betaine to grapevines at critical periods (e.g., prior to spring frosts) may protect plants and maintain yields [113]. However, high concentrations of glycine betaine can result in severe phytotoxicity for plants, and the concentrations for each plant species must be determined [112,113]. In grapevine, it is known that concentrations higher than 50 mM result in severe phytotoxicity [113]. Some studies have been developed regarding the application of glycine betaine in grapevines under drought. The effect of foliar applications of GB (15 mM) has been studied at four growth stages (before flowering, flowering, bunch closure, and veraison) under water stress (plants irrigated by 70% of the required water) [60] (Table 1). In a recent study in cherry, it was found that the foliar application of GB leads to an increase in the cuticle thickness [98]. These results are very promising for the application of glycine betaine in grapevine as the increase in the thickness of the cuticle may protect the berries against water loss and infection by fungi and also by pests. The application of GB in grapevine might be common in the near future since this compound can be easily acquired by farmers as it is a low-cost product.

Grapevine Responses to Biostimulants
Biostimulants can have three different modes of action: plant growth promoters/inhibitors, stress alleviators, and combined action [120]. These modes of action are dependent on the different bioactive compounds present in the biostimulants, of which little is known. Biostimulants can improve plant performance acting directly on the plant physiology and metabolism or by improving the soil conditions [121]. On the other hand, biostimulants can also act as elicitors, acting as stress alleviators, involved in resistance to drought, salinity, and in mechanisms of response to pathogens. The plant responses to biostimulant applications have been usually associated with the signaling of bioactive molecules in the primary and secondary metabolisms [120]. While the effects of these biostimulants are documented in several species, little is known about the mechanisms of action, due to the variable and complex nature of these substances. The beneficial effects of biostimulant applications can be associated with several biochemical and physiological mechanisms. In grapevine, the application of these compounds triggers responses in the cellular membrane, chloroplast, and nucleus protecting this species against biotic and abiotic stresses.
A summary of some mechanisms that can be triggered by the different biomolecules present in the biostimulants is shown in Figure 2. Some biostimulants act as elicitors, such as seaweed extracts (SE, green) and chitosan (CHT, orange); or as plant-derived elicitors, such as methyl jasmonate (MeJA, blue). Figure 2 schematically shows how these molecules act as elicitors. First, they act as pathogen-associated molecular patterns (PAMPs), which bind to host transmembrane pattern recognition receptors (PRRs). Then, plants are prepared for the induced systemic resistance (ISR) that occurs naturally, the defensive capacity being developed by a plant when appropriately stimulated. They also activate the systemic acquired resistance (SAR), characterized by an accumulation of salicylic acid (SA) and PR proteins. In the case of an ISR mechanism, jasmonic acid (JA) and ethylene (ET) pathways are important for the induction of broad-spectrum disease resistance. In fact, some authors verified that responses to B. cinerea attack in grapes are mediated by jasmonic acid and ethylene; while in SAR, accumulation of salicylic acid (SA) occurs both locally and, at low levels, systemically [107]. Salicylic acid plays a crucial role in the induction of several families of pathogenesis-related genes, such as β-1,3-glucanases and chitinases, capable of hydrolyzing fungal cell walls [14] ( Table 2). Plants sprayed with these biostimulants react quickly and plant cell membrane receptors bind to elicitor molecules, induce local resistance, and subsequently generate plant molecular responses. This fact can be explained because some biostimulants can be derived from plants that acquire resistance for their own diseases and now can act as elicitors when applied in other crops, such as grapevine.  Table 2. Genes involved in pathways triggered in grapevine upon application of biostimulants.
PR5 is a thaumatin-like/osmotin, and is a responsive antifungal protein that confers tolerance to both biotic and abiotic stresses in plants. PR10 may play an important role in the defense of grapevine against P. viticola. PR17 is related to defense responses. An accumulation of PR17 after P. viticola inoculation has been verified. PIN codifies for a serine-proteinase inhibitor (PIN), a class of antifungal PR-6 proteins, which have potent activity against pathogens. [32,[124][125][126][127] Polygalacturonase-inhibiting protein (PGIP) The PGIP gene product, a polygalacturase-inhibiting protein, interacts with extracellular endo-α-1,4-polygalacturonases (PGs), secreted by phytopathogenic fungi, to inhibit their activity. [32] Resveratrol Synthase (STS1) Involved in resveratrol and ε-viniferin production, the two major phytoalexinsin the protection against pathogens. [32] UDP-glucose:flavonoid 3-O-glucosyltransferase (UFGT) Key gene of the flavonoid biosynthetic pathway. [2,106] Another mechanism in Figure 2 refers to glycine betaine (GB, pink), which protects plants mostly against oxidative stress, which is caused by several adverse conditions. This generally occurs, when the balance between the production of reactive oxygen species (ROS) and the quenching activity is perturbed by an external stressor [27]. It is known that ROS are normally produced at low levels by various metabolic processes, such as photosynthesis or respiration, playing an important role in signaling related to the growth and development of plants [27]. Under stress conditions, the amount of ROS increases dramatically, which can result in cell damage, death, and toxicity to proteins, lipids, or nucleic acids. Through the activity of the antioxidant system, ROS concentration is maintained at non-toxic levels [27]. Biostimulants can activate antioxidative enzymatic function and increase ROS scavenging enzymes that are required to inactivate toxic free oxygen radicals produced in plants under stress, namely drought and salinity [15,27]. Glycine betaine can act in six different ways [15]: (1) GB limits efflux of K + ions induced by ROS, with the protection of membrane integrity or by a channel-blocking function; (2) this compound can protect the transcription machinery activating the genes for ROS-scavenging enzymes, reducing the ROS levels and the effects of abiotic stress in photosynthetic machinery; (3) the protection of the photodamaged PSII is induced by psbA gene that encodes the D1 protein; (4) GB participation in osmoregulation and in the protection of biological membranes integrity against the effects of abiotic stresses and ROS; (5) when GB biosynthesis consumes photosynthesis-generated electrons, may alleviate electron transport chain, which prevents overreduction of the photosynthetic electron transport chain, thus lowering the probability for generation of ROS; (6) the protection of the integrity of carbon-fixing enzymes, maintaining the higher rates of CO 2 fixation (PSI), even under stress.

Differential Expression of Genes Involved in Metabolic Grapevine Pathways upon Biostimulants Application
Several studies have been carried out to verify the molecular regulation and gene expression triggered by biostimulant applications in grapevine (see Table 2). Defenserelated genes and those involved in the regulation of secondary metabolism are among the most studied. On grapevine leaves cell suspensions of cv. Gamay treated with laminarin (derived from the brown alga Laminaria digitata) 11 defense-related genes were analyzed ( Table 2). Six genes were activated rapidly after laminarin treatment (LOX, GST, PAL, STS1, CHIT4c, and CHIT1b), whereas others were up-regulated later (CHIT3, PIN, GLU1, and PGIP). This study suggests that laminarin is an efficient elicitor of defense responses in grapevine, as it reduced the development of B. cinerea and P. viticola [32]. The exogenous application of abscisic acid in table grapes at different timings (7 or 21 days after veraison) and at different concentrations (200 or 400 mg L −1 ) was evaluated showing an increase in the expression of the anthocyanin biosynthetic genes (CHI, F3'H, DFR, and UFGT and of the VvMYBA1 and VvMYBA2 transcription factors) after two applications at 400 mg L −1 ( Table 2) [122]. Modulation of genes related to the flavonoid metabolic pathway (UFGT, OMT2, LDOX, GST, F3'H, F3'5'H, and DFR) has been also reported in cv. Sangiovese vines, subjected to multiple foliar applications of the brown alga A. nodosum extracts. These genes relative expression was influenced by the growing stage, as well as some defense-related genes (VvPR1 and VvCaS2) were up-regulated [51]. The foliar application of kaolin in grapevine, used as a strategy for summer stress mitigation, triggers metabolic pathways associated with the quality of the berry and, consequently, of the wines [48]. Dinis et al. [48] showed that kaolin exogenous application in cv. Touriga Nacional lowers ROS levels, increases hydroxyl radical scavenging, and enhances the production of antioxidant compounds, including phenolics, flavonoids, and anthocyanins. They showed increases in the transcript abundance of PAL1 and CHS1, genes apparently contributing to the changes in phenolic concentration. In chitosan-treated berries of cvs. Tinto Cão and Touriga Franca ROS pathway genes (AO, Fe-SOD, CAT, GR, Grx, Rboh, Cu/Zn-SOD, POD, and PPO) and the analysis of leaves, stems and shoots revealed that chitosan besides inducing the synthesis of phenolic compounds also acted as a facilitator for transfer of polyphenols from the leaves to the berries [56]. Moreover, in the cv. Tinto Cão, Singh et al. [57] found in chitosan treated leaves and berry skins an up-regulation of several target genes (i.e., PAL, UFGT, ABCC1, CHS, F3H, ANR, GST, and MATE1) that encode key enzymes and transporters involved in secondary metabolic pathways.

Conclusions
The worsening of climate change is very problematic for viticulture, increasing the biotic and abiotic stress and consequently compromising the grape quality and yield. Thus, it is urgently necessary to find successful mitigation strategies. In the future, the use of biostimulants will be very important to reduce the use of synthetic chemicals in viticulture. Currently, many biostimulants are being used in grapevines to improve plant physiology and metabolism or act as elicitors. They act as stress alleviators, involved in the resistance against, for example, drought, heat, high irradiation, and also in mechanisms of response to pathogens. In order to use biostimulants with maximum efficiency, it will be important to identify the different mechanisms of action of their bioactive compounds. In addition, the mechanisms triggered by these biostimulants in plants are still poorly understood. There should be a focus on studying different grapevine cultivars and terroirs, as the responses to the application of the same biostimulants may be different depending on the conditions to which the plant is exposed. It will also be important to define the safe concentrations of these biostimulants, the best strategy of application (foliar or other), the number of applications, the phenological stages at which they should be applied, and which biotic or abiotic stress they protect from. Moreover, research on new biostimulant compounds and formulations must be the main goal in order to make viticulture more environment-friendly.