Application of Rhizobacteria, Paraburkholderia fungorum and Delftia sp. Confer Cadmium Tolerance in Rapeseed (Brassica campestris) through Modulating Antioxidant Defense and Glyoxalase Systems

We investigated the role of two different plant growth-promoting probiotic bacteria in conferring cadmium (Cd) tolerance in rapeseed (Brassica campestris cv. BARI Sarisha-14) through improving reactive oxygen species scavenging, antioxidant defense, and glyoxalase system. Soil, as well as seeds of rapeseed, were separately treated with probiotic bacteria, Paraburkholderia fungorum BRRh-4 and Delftia sp. BTL-M2. Fourteen-day-old seedlings were exposed to 0.25 and 0.5 mM CdCl2 for two weeks. Cadmium-treated plants resulted in a higher accumulation of hydrogen peroxide, increased lipid peroxidation, electrolyte leakage, chlorophyll damage, and impaired antioxidant defense and glyoxalase systems. Consequently, it reduced plant growth and biomass production, and yield parameters. However, probiotic bacteria-inoculated plants significantly ameliorated the Cd toxicity by enhancing the activities of antioxidant enzymes (ascorbate peroxidase, dehydroascorbate reductase, monodehydroascorbate reductase, glutathione reductase, glutathione peroxidase, and catalase) and glyoxalase enzymes (glyoxalase I and glyoxalase II) which led to the mitigation of oxidative damage indicated by reduced hydrogen peroxide, lipid peroxidation, and electrolyte leakage that ultimately improved growth, physiology, and yield of the bacterial inoculants rapeseed plants. When taken together, our results demonstrated the potential role of the plant probiotic bacteria, BRRh-4 and BTL-M2, in mitigating the Cd-induced damages in rapeseed plants.


Introduction
The rapid increase in metal pollution during the recent decades poses a severe threat to all living organisms. The primary reason behind this situation is the rapid growth of industries that indiscriminately excrete toxic metals/metalloids to nature and disrupt the equilibrium of the ecological components [1]. Among the metals/metalloids, cadmium (Cd) is the most toxic metal having a damaging effect on both plants and animals. The concentration of Cd beyond 5-10 mg g −1 dry weight of leaf creates phytotoxicity in the plants. Due to the hydrophilic nature of Cd, it could be rapidly accumulated through the roots and translocated easily to different tissues of plants, thus threatening the food chain of living organisms [2,3].
Cadmium toxicity is responsible for the alteration of the physiological processes of plants by creating obstacles in carbon fixation and photosynthetic pigment synthesis. with this plant probiotic bacteria had no fungal disease infestation [24]. Biodegradation of fungicide and heavy metal removal by Burkholderia fungorum FM-2 and some species of Paraburkholderia spp. have been reported [25]. On the other hand, Delftia spp. are known to have heavy metal reducing and atmospheric nitrogen fixing abilities [26]. However, the roles of P. fungorum BRRh-4 and Delftia sp. BTL-M2 on plants' tolerance to Cd-spiked soils are unknown. Therefore, we hypothesized that P. fungorum BRRh-4 and Delftia sp. BTL-M2 confer Cd tolerance in rapeseed. Thus, this study aimed to investigate the potentiality of P. fungorum and Delftia sp. in diminishing oxidative stress caused by Cd toxicity through upregulating defensive responses through coordinated actions of antioxidant defense and glyoxalase system in B. campestris.

Plant Materials, Experimental Conditions, and Treatments
Healthy and matured seeds of rapeseed (B. campestris cv. BARI Sarisha-14) were collected from Bangladesh Agriculture Research Institute (BARI), surface sterilized, and sown in well-prepared soil after applying the recommended dose of fertilizers [27] in the plastic pot (14 L). The plant growth-promoting probiotic bacteria, P. fungorum BRRh-4 and Delftia sp. BTL-M2 were isolated from the roots of rice and rice soils, respectively. The probiotic bacteria (BRRh-4 and BTL-M2) were cultivated separately in 500 mL nutrient broth (Merck, Germany) in conical flasks taking a single colony from actively growing bacterial culture plates that were maintained on a lab bench at ambient temperature (25 ± 2 • C) [24]. Then each flask was placed on a shaking incubator adjusted at 120 rpm and 25 • C for 72 h for bacterial growth in nutrient broth. The broth was centrifuged at 12,000× g, and the pellet was washed thrice with sterilized distilled water to remove nutrients. The bacterial pellet was suspended in water and diluted to a concentration of approx. 1 × 10 9 CFU mL −1 and 5 × 10 8 CFU mL −1 for P. fungorum BRRh-4 and Delftia sp. BTL-M2, respectively, according to the procedure of Rahman et al. [24]. Before sowing, seeds were treated with either P. fungorum BRRh-4 (ca. 1 × 10 9 CFU mL −1 ) or Delftia sp. BTL-M2 (ca. 5 × 10 8 CFU mL −1 ). After proper intercultural operations 14 days after sowing (DAS), sets of plants were subjected to two concentrations of Cd stress by adding 0.25 and 0.5 mM cadmium chloride (CdCl 2 ). After growing for another 14 days in the Cd-supplemented conditions, growth, physiological, and biochemical parameters were recorded. The experiment was laid out in a completely randomized design (CRD) with three replications. One set of pots was used for estimating morphological, physiological, and biochemical parameters, and another group of plants was used to determine the yield and yield contributing parameters.

Measurement of Plant Height, SPAD Value, and Biomass Accumulation
Five plants were selected instinctively from each treatment and measured with a scale. The average value was expressed as centimeters (cm).
The reading of soil and plant analysis development (SPAD) was taken by using a SPAD meter (FT Green LLC, Wilmington, DE, USA) from five randomly selected fully expanded leaves to estimate the chlorophyll (Chl) content of the plants. Then the values were averaged to express the SPAD value.
At the junction of roots and shoots were excised and weighed in an electric balance for the estimation of fresh weight (FW). Hereafter, the roots and shoots were sun-dried, followed by oven dried at 80 • C for 72 h and weighed again for the dry weight (DW). Then, the mean value of the FW and DW of root and shoots were expressed as g plant −1 .

Estimation of Relative Water Content
The relative water content (RWC) of the leaf was determined following the formula of Barrs and Weatherly [28]. The calculating formula is as follows: Relative water content (%) = Fresh weight (FW) − Dry weight (DW) Turgid weight (TW) − Dry weight (DW) × 100 For estimating the FW, three leaf lamina were taken randomly from each treatment and weighed. Afterward, these leaves were placed in a Petri dish with adequate distilled water (dH 2 O) and covered with filter paper in a dark place for re-saturation. After 24 h of soaking in the dH 2 O, leaves were removed from the Petri dish, and excess water was wiped with tissue paper. Then turgid weight (TW) of the leaves was measured, followed by oven drying of those leaves at 80 • C for 72 h to determine the DW. After recording all the data, the RWC of the leaf was measured by using the above-mentioned formula.

Quantification of Proline Content
Proline (Pro) was determined according to the method of Bates et al. [29]. Fresh leaf of 0.5 g was taken and homogenized with 5 mL of 3% sulfosalicylic acid followed by centrifugation at 11,500× g for 15 min at 4 • C. After that, 1 mL of acid ninhydrin and 1 mL of glacial acetic acid were mixed with 1 mL of aliquot which was separated after centrifugation. This mixture was incubated in a water bath at 100 • C for 1 h. After cooling to room temperature, 4 mL of toluene was added to the mixture to separate the free Pro. The absorbance of the colored chromophore was measured spectrophotometrically at 520 nm using toluene as a blank. Finally, the Pro content was calculated with respect to a standard curve made with the known concentration of L-proline and expressed as µmol g −1 FW.

Estimation of Electrolyte Leakage
Electrolyte leakage (EL) of the leaf was estimated according to the method of Dionisio-Sese and Tobita [30] following the formula: Firstly, a harvested leaf of 0.5 g was taken into a Falcon tube and filled with 15 mL of dH 2 O. After that, each Falcon tube was incubated in the water bath at 40 • C for 1 h. The initial electrical conductivity (EC 1 ) was measured with an electrical conductivity (EC) meter (HI-993310, Hanna, RI, USA) after cooling at room temperature. These Falcon tubes were incubated in an autoclave at 121 • C for 20 min, then cooled in an ice bath to room temperature. Final conductivity (EC 2 ) was taken again with the EC meter, and the calculation of EL of the leaf was done following the above-mentioned formula.

Determination of Stress Indicators
For estimating malondialdehyde (MDA) content, 0.5 g of the fresh leaf was mashed with 3 mL of 5% (w/v) trichloroacetic acid (TCA) using chilled mortar and pestle and centrifuged for 15 min at 11,500× g. Then the 1 mL of the obtained aliquot after centrifugation was consolidated with 4 mL of thiobarbituric acid (TBA) reagent that contained 0.5% of TBA dissolved in the 20% TCA according to the procedure of Heath and Packer [31]. After being heated at 95 • C for 30 min in a water bath, the reaction mixture was promptly chilled in an ice bath. The absorbance of the colored chromophore was observed at 532 nm and adjusted for non-specific absorbance at 600 nm. After using the extinction coefficient of 155 mM −1 cm −1 , the amount of MDA content was estimated and expressed as nmol g −1 FW.
The hydrogen peroxide (H 2 O 2 ) content was measured using the technique by Yu et al. [32]. At 4 • C, 0.5 g of leaf tissue was macerated with mortar and pestle in 5% TCA and centrifuged for 15 min at 11,500× g to obtain a clear aliquot. Afterward, 1 mL of the obtained aliquot was fused with 1 mL of potassium-phosphate (K-P) buffer pH 7 (10 mM) and 1 mL of potassium iodide (1 mM), then the mixture was kept in the dark for 1 h. In order to quantify the H 2 O 2 , the optical density of the mixture was taken at 390 nm by spectrophotometer, and the calculated value of H 2 O 2 content was expressed as nmol g −1 FW.

Extraction and Quantification of Protein Content and Enzymes Activity Assay
Fresh leaf (0.5 g) was macerated in a pre-cooled mortar pestle using 1 mL of extraction buffer, which contained 50 mM K-P buffer (pH 7.0), 100 mM potassium chloride (KCl), 1 mM L-ascorbic acid (Asc), 5 mM β-mercaptoethanol, and 10% (w/v) glycerol, and subjected for centrifugation at 4 • C (11,500× g, 10 min). Then, the obtained aliquot was used to determine protein concentration and enzyme activity [33].
The protein content was determined according to the method of Bradford [34] using bovine serum albumin (BSA) as a protein standard. At first, the Bradford reagent was prepared with the Coomassie brilliant blue (G-250), ethanol (100%), phosphoric acid (85%), and dH 2 O. Then, 5 µL of the aliquot and 5 mL of Bradford reagent were incorporated, and the absorbance of the mixture was observed at 595 nm by spectrophotometer. Afterward, the absorbance of the unknown sample was plotted against a standard curve prepared from the known concentration of BSA, and the protein concentration was determined.
The APX (EC: 1.11.1.11) activity was measured using Nakano and Asada's [35] technique. In a final volume of 0.7 mL, the reaction buffer solution contained K-P buffer (50 mM, pH 7.0), Asc (0.5 mM), H 2 O 2 (0.1 mM), ethylenediaminetetraacetic acid (EDTA; 0.1 mM), and enzyme extract. The H 2 O 2 was used to initiate the reaction, and an extinction coefficient of 2.8 mM −1 cm −1 was used to assess activity by measuring the decline in absorbance for 1 min at 290 nm spectrophotometrically.
The MDHAR (EC: 1.6.5.4) activity was determined by the method of Hossain et al. [36]. The reaction mixture contained Tris-HCl buffer (50 mM, pH 7.5), nicotinamide adenine dinucleotide phosphate (NADPH; 0.2 mM), Asc (2.5 mM), ascorbate oxidase (AO; 0.5 U), and the total volume of the enzyme mixture was 0.7 mL. After the addition of AO to the mixture, the reaction was initiated. The ascorbate's changing activity was determined at 340 nm by a spectrophotometer for 1 min using an extinction coefficient of 6.2 mM −1 cm −1 .
The DHAR (EC: 1.8.5.1) activity was assessed using the Nakano and Asada method [35]. The K-P buffer (50 mM, pH 7.0), reduced glutathione (GSH; 2.5 mM), and dehydroascorbate (DHA; 0.1 mM) were present in the reaction buffer. The enzyme solution was added to the reaction buffer to initiate the reaction. In using an extinction value of 14 mM −1 cm −1 , the activity was determined from the change in absorbance for 1 min at 265 nm spectrophotometrically.
The GR (EC: 1.6.4.2) activity was assessed using the Hasanuzzaman et al. [33] technique. In a final volume of 1 mL, the reaction mixture contained K-P buffer (0.1 M, pH 7.8), EDTA (1 mM), oxidized glutathione (GSSG; 1 mM), NADPH (0.2 mM), and enzyme solution. The GSSG was used to start the reaction, and for 1 min, the NADPH oxidation-related drop in absorbance at 340 nm was observed in a spectrophotometer. In utilizing an extinction value of 6.2 mM −1 cm −1 , the activity was computed.
The CAT (EC: 1.11.1.6) activity was assessed by observing the drop in absorbance at 240 nm for 1 min brought by the breakdown of H 2 O 2 . In a final volume of 0.7 mL, the reaction mixture contained K-P buffer (50 mM, pH 7.0), H 2 O 2 (15 mM), and enzyme solution. After adding H 2 O 2 to the reaction mixture, the enzyme started the reaction and computed the activity by using 39.4 M −1 cm −1 as an extinction coefficient [33].
The Gly I (EC: 4.4.1.5) enzyme assay mixture, which had a final volume of 0.7 mL, was composed of K-P buffer (100 mM, pH 7.0), magnesium sulfate (MgSO 4 ; 15 mM), GSH (1.7 mM), and MG (3.5 mM). The addition of MG triggered the process, and the rise in absorbance was observed at 240 nm for 1 min spectrophotometrically, and utilizing an extinction value of 3.37 mM −1 cm −1 , the activity was computed [33].
The production of GSH at 412 nm for 1 min in a spectrophotometer was used to measure the activity of Gly II (EC: 3.1.2.6) following the procedure of Principato et al. [38]. In a final volume of 1 mL, the reaction mixture contained Tris-HCl buffer (100 mM, pH 7.2), 5,5dithio-bis(2-nitrobenzoic acid) (DTNB; 0.2 mM), and S-D -lactoylglutathione (SLG; 1 mM). The SLG was used to initiate the reaction, and the activity was determined using the 13.6 mM −1 cm −1 extinction coefficient.

Statistical Analysis
The parameters were studied from three replications, and the results were presented as mean values with standard deviations (±SD). The significant differences among the treatments were statistically analyzed by using the CoStat v.6.400 computer-based software (Co-Hort Software, Monterey, CA, USA) after applying Tukey's HSD test at p ≤ 0.05 level of significance [39]. Correlation analysis was done using Origin Pro 2022 (OriginLab, Northampton, MA, USA).

Growth Parameters and Photosynthetic Activity
Plants treated with Cd 0.25 and Cd 0.5 reduced the plant height by 17 and 61%, respectively, compared to the controls ( Figure 1A). However, P. fungorum and Delftia sp. inoculation did not enhance the height at Cd 0.25 -and Cd 0.5 -treated plants. Compared to controls, the SPAD value was reduced by 9% in Cd0.25-and 26% in Cd0.5stressed plants ( Figure 1B). The supplementation of P. fungorum enhanced the value by 9 and 20%, respectively. In contrast, the value increased by 14 and 16% with the Delftia sp., respectively, in the Cd0.25-and Cd0.5-stressed plants compared to the corresponding Cd treated plants.
Under Cd toxicity, biomass accumulation was also suppressed. Under Cd0.25, the root Compared to controls, the SPAD value was reduced by 9% in Cd 0.25 -and 26% in Cd 0.5 -stressed plants ( Figure 1B). The supplementation of P. fungorum enhanced the value by 9 and 20%, respectively. In contrast, the value increased by 14 and 16% with the Delftia sp., respectively, in the Cd 0.25 -and Cd 0.5 -stressed plants compared to the corresponding Cd treated plants.
Under Cd toxicity, biomass accumulation was also suppressed. Under Cd 0.25 , the root FW, shoot FW, root DW, and shoot DW were decreased by 20, 31, 33, and 32%, respectively. On the contrary, the reduction was 70, 85, 67, and 89%, respectively, when treated with Cd 0.5 ( Figure 1C-F). In the P. fungorum inoculated Cd 0.25 -stressed plants, the shoot FW (23%), root DW (28%), and shoot DW (25%) were increased compared to the uninoculated Cd-treated plants. Similarly, the Delftia sp. inoculation only enhanced the shoot FW (26%) and DW (22%) in the Cd 0.25 -treated plants, respectively, compared to the uninoculated Cd-treated plants. At the Cd 0.5 -treated plants, the inoculation of either P. fungorum or Delftia sp. did not significantly increase the FW and DW of roots and shoots.

Relative Water Content and Proline Accumulation
Upon exposure to Cd stress, the RWC of leaf reduced by 19% in Cd 0.5 -stressed plants in comparison to controls, whereas no change was observed under Cd 0.25 ( Figure 2A). However, the leaf RWC did not enhance significantly with the inoculation of P. fungorum and Delftia sp. in the Cd 0.25 -and Cd 0.5 -stressed plants. A steady increase of Pro content by 165 and 292% at Cd0.25 and Cd0.5, respectively, compared to controls ( Figure 2B). The P. fungorum decreased the Pro content by 37 and 41%, while Delftia sp. decreased it by 30 and 19%, respectively, in plants treated with Cd0.25 and Cd0.5 compared to the Cd alone.

Oxidative Stress Indicators
Oxidative stress indicators, the MDA content increased by 60 and 122% at Cd0.25 and Cd0.5, while the increment of H2O2 content was found by 24 and 48%, respectively, compared to control plants. Similarly, the EL was also upgraded by 25 and 33% at Cd0.25 and Cd0.5 stress, respectively. ( Figure 3A-C). However, the P. fungorum reduced the MDA content by 40% in Cd0.25-and 15% in Cd0.5-stressed plants, respectively, compared to the corresponding Cd treated alone. In contrast, the Delftia sp. only reversed the MDA content by 24% in Cd0.25-stressed plants but showed no improvement at Cd0.5 ( Figure 3A). The H2O2 content decreased by 22 and 14%, respectively, with the P. fungorum and Delftia sp. inoculation at Cd0.25 compared to Cd alone ( Figure 3B). Conversely, the bacterial inoculation did not decrease the H2O2 content significantly at Cd0.5 stress. A similar reduction of the EL was also observed with the P. fungorum (13 and 9%) and Delftia sp. (9 and 5%) in plants with Cd0.25 and Cd0.5 stress ( Figure 3C). A steady increase of Pro content by 165 and 292% at Cd 0.25 and Cd 0.5 , respectively, compared to controls ( Figure 2B). The P. fungorum decreased the Pro content by 37 and 41%, while Delftia sp. decreased it by 30 and 19%, respectively, in plants treated with Cd 0.25 and Cd 0.5 compared to the Cd alone.

Oxidative Stress Indicators
Oxidative stress indicators, the MDA content increased by 60 and 122% at Cd 0.25 and Cd 0.5 , while the increment of H 2 O 2 content was found by 24 and 48%, respectively, compared to control plants. Similarly, the EL was also upgraded by 25 and 33% at Cd 0.25 and Cd 0.5 stress, respectively. ( Figure 3A-C). However, the P. fungorum reduced the MDA content by 40% in Cd 0.25 -and 15% in Cd 0.5 -stressed plants, respectively, compared to the corresponding Cd treated alone. In contrast, the Delftia sp. only reversed the MDA content by 24% in Cd 0.25 -stressed plants but showed no improvement at Cd 0.5 ( Figure 3A). The H 2 O 2 content decreased by 22 and 14%, respectively, with the P. fungorum and Delftia sp. inoculation at Cd 0.25 compared to Cd alone ( Figure 3B). Conversely, the bacterial inoculation did not decrease the H 2 O 2 content significantly at Cd 0.5 stress. A similar reduction of the EL was also observed with the P. fungorum (13 and 9%) and Delftia sp. (9 and 5%) in plants with Cd 0.25 and Cd 0.5 stress ( Figure 3C). by 24% in Cd0.25-stressed plants but showed no improvement at Cd0.5 ( Figure 3A). Th H2O2 content decreased by 22 and 14%, respectively, with the P. fungorum and Delftia sp inoculation at Cd0.25 compared to Cd alone ( Figure 3B). Conversely, the bacterial inocula tion did not decrease the H2O2 content significantly at Cd0.5 stress. A similar reduction o the EL was also observed with the P. fungorum (13 and 9%) and Delftia sp. (9 and 5%) in plants with Cd0.25 and Cd0.5 stress ( Figure 3C).

Activities of Antioxidant Enzymes
Cadmium stress augmented the activity of APX (20 and 47%) and GR (19 and 30%), whereas it suppressed the MDHAR (26 and 50%) and DHAR (23 and 33%) activities at Cd 0.25 and Cd 0.5 , respectively, in comparison to controls ( Figure 4A-D). The APX activity was further enhanced by 33 and 26% with the P. fungorum in the plants treated with Cd 0.25 and Cd 0.5 stress, respectively. Delftia sp., on the other hand, only improved the activity of APX by 17% with Cd 0.25 -treated plants compared to Cd only ( Figure 4A). Compared to the uninoculated Cd stressed plants, the P. fungorum and Delftia sp. increased the MDHAR activity by 46 and 29%, and by 35 and 27%, respectively, in the plants at Cd 0.25 and Cd 0.5 stress ( Figure 4B). Likewise, the P. fungorum upgraded the activity of DHAR by 24 and 10% and GR by 16 and 13%, respectively, in the Cd 0.25 and Cd 0.5 stress in comparison to Cd treated alone. However, the Delftia sp. also augmented the DHAR activity by 10% in Cd 0.25 and 15% in Cd 0.5 and uplifted the GR activity only at Cd 0.25 stress by 13% compared to corresponding Cd stressed only ( Figure 4C,D).
The activity of GPX was declined by 23 and 38% in the plant treated with Cd 0.25 and Cd 0.5 , respectively, compared to controls. However, the GPX activity was reversed with P. fungorum (30 and 32%) and Delftia sp. (15 and 14%) at Cd 0.25 and Cd 0.5 compared to Cd only ( Figure 5A). A similar trend was observed in the case of CAT activity under Cd stress. However, the activity was enhanced with P. fungorum and Delftia sp. by 52 and 48% at Cd 0.25 , whereas at Cd 0.5 , it was increased by 44% only with the P. fungorum, compared to Cd treated alone ( Figure 5B).
uninoculated Cd stressed plants, the P. fungorum and Delftia sp. increased the MDHAR activity by 46 and 29%, and by 35 and 27%, respectively, in the plants at Cd0.25 and Cd0.5 stress ( Figure 4B). Likewise, the P. fungorum upgraded the activity of DHAR by 24 and 10% and GR by 16 and 13%, respectively, in the Cd0.25 and Cd0.5 stress in comparison to Cd treated alone. However, the Delftia sp. also augmented the DHAR activity by 10% in Cd0. 25 and 15% in Cd0.5 and uplifted the GR activity only at Cd0.25 stress by 13% compared to corresponding Cd stressed only ( Figure 4C,D). The activity of GPX was declined by 23 and 38% in the plant treated with Cd0.25 and Cd0.5, respectively, compared to controls. However, the GPX activity was reversed with P. fungorum (30 and 32%) and Delftia sp. (15 and 14%) at Cd0.25 and Cd0.5 compared to Cd only ( Figure 5A). A similar trend was observed in the case of CAT activity under Cd stress. However, the activity was enhanced with P. fungorum and Delftia sp. by 52 and 48% at Cd0.25, whereas at Cd0.5, it was increased by 44% only with the P. fungorum, compared to Cd treated alone ( Figure 5B).

Activities of Glyoxalase Enzymes
Both Gly I and Gly II activities were decreased with the gradual increase of Cd doses. The activity of Gly I and Gly II declined by 24 and 44% and by 23 and 47% in the plants treated with the Cd0.25 and Cd0.5 stress, respectively, compared to the controls. However, the augmentation of the Gly I activity by 28 and 25% and Gly II activity by 27 and 24% in the P. fungorum inoculated plants treated with the Cd0.25 and Cd0.5 stress, respectively, in comparison to the corresponding Cd alone. In contrast, the Delftia sp. inoculation only enhanced the Gly I (18%) and Gly II (30%) activities at Cd0.25, respectively, compared to the Cd alone, but no changes were observed at Cd0.5-stressed plants ( Figure 6A,B).

Activities of Glyoxalase Enzymes
Both Gly I and Gly II activities were decreased with the gradual increase of Cd doses. The activity of Gly I and Gly II declined by 24 and 44% and by 23 and 47% in the plants treated with the Cd 0.25 and Cd 0.5 stress, respectively, compared to the controls. However, the augmentation of the Gly I activity by 28 and 25% and Gly II activity by 27 and 24% in the P. fungorum inoculated plants treated with the Cd 0.25 and Cd 0.5 stress, respectively, in comparison to the corresponding Cd alone. In contrast, the Delftia sp. inoculation only enhanced the Gly I (18%) and Gly II (30%) activities at Cd 0.25 , respectively, compared to the Cd alone, but no changes were observed at Cd 0.5 -stressed plants ( Figure 6A,B).

Activities of Glyoxalase Enzymes
Both Gly I and Gly II activities were decreased with the gradual increase of Cd doses. The activity of Gly I and Gly II declined by 24 and 44% and by 23 and 47% in the plants treated with the Cd0.25 and Cd0.5 stress, respectively, compared to the controls. However, the augmentation of the Gly I activity by 28 and 25% and Gly II activity by 27 and 24% in the P. fungorum inoculated plants treated with the Cd0.25 and Cd0.5 stress, respectively, in comparison to the corresponding Cd alone. In contrast, the Delftia sp. inoculation only enhanced the Gly I (18%) and Gly II (30%) activities at Cd0.25, respectively, compared to the Cd alone, but no changes were observed at Cd0.5-stressed plants ( Figure 6A,B).

Yield Parameters
The toxicity of Cd considerably declined the yield parameters of the rapeseed plants. The number of siliqua plant −1 was decreased by 28 and 73% at Cd0.25 and Cd0.5, respectively, compared to the controls. However, the P. fungorum only increased the number of siliqua plant −1 in the Cd0.5 treated plants by 55% compared to the corresponding uninoculated Cd stressed only. The siliqua length declined at Cd0.5 by 48% compared to the controls. However, the P. fungorum increased the siliqua length by 40% at Cd0.5 stress compared to the respective Cd treated alone. Similarly, the number of seeds siliqua −1 (35 and 53%), 1000seed weight (19 and 77%), and seed yield plant −1 (32 and 89%) were declined when treated

Yield Parameters
The toxicity of Cd considerably declined the yield parameters of the rapeseed plants. The number of siliqua plant −1 was decreased by 28 and 73% at Cd 0.25 and Cd 0.5 , respectively, compared to the controls. However, the P. fungorum only increased the number of siliqua plant −1 in the Cd 0.5 treated plants by 55% compared to the corresponding uninoculated Cd stressed only. The siliqua length declined at Cd 0.5 by 48% compared to the controls. However, the P. fungorum increased the siliqua length by 40% at Cd 0.5 stress compared to the respective Cd treated alone. Similarly, the number of seeds siliqua −1 (35 and 53%), 1000-seed weight (19 and 77%), and seed yield plant −1 (32 and 89%) were declined when treated with Cd 0.25 and Cd 0.5 stress, respectively, in comparison to the control plants. However, the P. fungorum improved the number of seeds siliqua −1 by 46 and 39% at Cd 0.25 and Cd 0.5 , respectively. In contrast, the Delftia sp. only increased it at Cd 0.25 by 27% compared to the Cd alone. Likewise, the P. fungorum enhanced 1000-seed weight by 18 and 97%, and Delftia sp. by 17 and 62%, respectively, in plants treated with the Cd 0.25 and Cd 0.5 stress compared to the respective Cd treated alone. Furthermore, the seed yield plant −1 also enhanced with the P. fungorum by 40 and 204%, respectively, at Cd 0.25 and Cd 0.5 , whereas the Delftia sp. only increased the seed yield plant −1 at Cd 0.25 treated plants by 27% in comparison to the corresponding uninoculated Cd alone ( Figure 7A-E).
respectively. In contrast, the Delftia sp. only increased it at Cd0.25 by 27% compared to the Cd alone. Likewise, the P. fungorum enhanced 1000-seed weight by 18 and 97%, and Delftia sp. by 17 and 62%, respectively, in plants treated with the Cd0.25 and Cd0.5 stress compared to the respective Cd treated alone. Furthermore, the seed yield plant −1 also enhanced with the P. fungorum by 40 and 204%, respectively, at Cd0.25 and Cd0.5, whereas the Delftia sp. only increased the seed yield plant −1 at Cd0.25 treated plants by 27% in comparison to the corresponding uninoculated Cd alone ( Figure 7A-E).

Correlation Analysis among the Parameters
The correlation analysis among the different parameters is depicted in Figure 8. From the matrix, it can be stated that the parameters of oxidative stress indicators, such as MDA, H2O2, and EL, are negatively correlated with the MDHAR, DHAR, GPX, CAT, Gly I, and Gly II activities. In contrast, the APX and GR activities are positively interlinked. Thus,

Correlation Analysis among the Parameters
The correlation analysis among the different parameters is depicted in Figure 8. From the matrix, it can be stated that the parameters of oxidative stress indicators, such as MDA, H 2 O 2 , and EL, are negatively correlated with the MDHAR, DHAR, GPX, CAT, Gly I, and Gly II activities. In contrast, the APX and GR activities are positively interlinked. Thus, the plant growth, biomass production, and yield components are negatively affected by the Cd-induced oxidative stress indicators (Figure 8). the plant growth, biomass production, and yield components are negatively affected by the Cd-induced oxidative stress indicators (Figure 8).

Discussion
Cadmium ubiquitously presents in nature which is a highly phytotoxic compound that may cause significant yield reductions and is also carcinogenic for animals and humans [3]. The toxic effect of Cd leads to growth inhibition and alters different biochemical processes, viz., photosynthetic activities, stomatal movements, carbon dioxide fixation, etc. [8]. Under Cd stress, the accumulation of iron [Fe(III)] becomes limited, thus inhibiting the activity of Fe(III) reductase, which abruptly affects photosynthesis and distorts the growth and biomass accumulation of the plants [8]. The retardation of growth attributes and leaf chlorosis due to impaired photosynthesis were also reported in the Cd-stressed Brassica napus [15], Zea mays [16], and Solanum nigrum [14] seedlings. In the current study, we demonstrated that two rice-associated growth-promoting bacteria, P. fungorum BRRh-4 and Delftia sp. BTL-M2 confer cadmium tolerance in rapeseed (B. campestris) plants, likely through modulating the antioxidant defense and glyoxalase systems in the plant Here, PHT-plant height; SPAD-soil and plant analysis development value; FW-fresh weight; DWdry weight; RWC-relative water content; Pro-proline; MDA-malondialdehyde; H 2 O 2 -hydrogen peroxide; EL-electrolyte leakage; APX-ascorbate peroxidase; MDHAR-monodehydroascorbate reductase; DHAR-dehydroascorbate reductase; GR-glutathione reductase; GPX-glutathione peroxidase; CAT-catalase; Gly I-glyoxalase I; Gly II-glyoxalase II; TSW-thousand seed weight.

Discussion
Cadmium ubiquitously presents in nature which is a highly phytotoxic compound that may cause significant yield reductions and is also carcinogenic for animals and humans [3]. The toxic effect of Cd leads to growth inhibition and alters different biochemical processes, viz., photosynthetic activities, stomatal movements, carbon dioxide fixation, etc. [8]. Under Cd stress, the accumulation of iron [Fe(III)] becomes limited, thus inhibiting the activity of Fe(III) reductase, which abruptly affects photosynthesis and distorts the growth and biomass accumulation of the plants [8]. The retardation of growth attributes and leaf chlorosis due to impaired photosynthesis were also reported in the Cd-stressed Brassica napus [15], Zea mays [16], and Solanum nigrum [14] seedlings. In the current study, we demonstrated that two rice-associated growth-promoting bacteria, P. fungorum BRRh-4 and Delftia sp. BTL-M2 confer cadmium tolerance in rapeseed (B. campestris) plants, likely through modulating the antioxidant defense and glyoxalase systems in the plant cells. Although growth and yield-promoting effects of P. fungorum and Delftia sp. have been reported [23,24,26], this study, for the first time, demonstrated that these rice probiotic bacteria enhance plant tolerance to a heavy metal via strengthening the antioxidant defense system. Amelioration of Cd toxicity by metal-resistant endophytic bacteria by immobilizing or modifying the availability of metals and enhancing the regulation of the essential nutrient uptake to the plants by synthesizing exopolysaccharides (EPS), siderophores production, acidification, or solubilizing phosphates have previously been reported [40]. Notably, plant growth (root length, shoot length, FW, and DW) and SPAD value were retrieved with supplementation of Enterobacter sp. in B. napus under Cd stress, as reported by Saeed et al. [15]. Under Cd stress, the Enterobacter-inoculated plants showed improved photosynthetic rate (P n ), transpiration rate (T r ), and stomatal conductance (g s ), thus enhancing the growth and biomass production of B. napus [15]. Likewise, Tanwir et al. [16] demonstrated inoculation of Serratia sp. incremented plant height, Chl content, and biomass production of the Cd-affected maize plants compared to the uninoculated plants. Moreover, Gupta et al. [41] confirmed that such growth promotion by inoculating Bacillus sp. bacteria was attenuated by increased accumulation of indole acetic acid (IAA), phosphate solubilization, and siderophore production under salt stress. Furthermore, Chi et al. [14] evaluated the gene expression of IRT1, HMA, and PDR2 that regulate Cd uptake, transport, and detoxification. They reported that in the Cd-stressed S. nigrum, the expression of IRT1 increased and suppressed the HMA, which led to a limit of the Cd deposition in the roots rather than shoots [14]. In addition to these, the microbe triggers phytohormone production and facilitates in the Cd detoxification system [42,43]. Chi et al. [14] also reported similar stimulation of the plant growth and root development by stimulating the IAA accumulation in Cd-spiked S. nigrum plants with the inoculation of B. megaterium. These results confirmed the PGPR-induced resistance toward the abrupt environmental conditions and improved growth and biomass production supported by the present study with the rhizobacteria, P. fungorum, and Delftia sp. inoculated rapeseed plants under Cd stress.
Osmotic balance is disrupted under metal/metalloid toxicity due to secondary effects. Therefore, plants accumulate secondary metabolites such as Pro to cope with the changing water balance, which is an effective osmoprotectant and aids in chelating metal ions [44]. In the current study, under Cd stress, the RWC of rapeseed leaves is reduced in a dosedependent manner which is in congruence with the previous findings of Cd-stressed B. napus [15]. However, in the present study, cellular dehydration has been ameliorated with the addition of P. fungorum and Delftia sp. in the Cd-stressed rapeseed plants. Likewise, bacterial inoculation of Enterobacter sp. enhanced the water content of B. napus, as reported by Saeed et al. [15] under Cd toxicity. Reports on the endophytic bacteria Burkholderia phytofirmans showed that water stress was alleviated in the salt-stressed Chenopodium quinoa plants [45]. Thus, to adjust the water balance, biosynthesis of osmolytes such as Pro is a key strategy to negate the osmotic shock under Cd stress [46]. The higher accumulation of Pro in response to Cd in the current investigation is supported by the previous findings [47,48]. However, in response to Cd stress, the P. fungorum and Delftia sp. inoculated rapeseed plants and enhanced water content mitigated Pro accumulation in the Cd stressed plants. Moreover, Lastochkina et al. [49] reported that B. subtilis inoculation in response to salinity stress and Pro accumulation was reduced in Phaseolus vulgaris under salinity. Similar retardation of Pro content was also found with the endophytic inoculation of B. subtilis in the salt-affected Triticum aestivum plants [50]. Moreover, microbes accelerated the production of EPS which enhanced adherence of soil particles and also aided in promoting macropore production to improve the soil porosity and aeration under stressful environments, thus maintaining osmotic adjustment [51].
Upon exposure to Cd stress, excessive generation of ROS such as H 2 O 2 , 1 O 2 , and O 2 •− , etc., leads to oxidative stress that distorts proteins, lipids, amino acids, and nucleic acids. The MDA content is a prime indicator of oxidative stress produced due to membrane damage resulting from lipid peroxidation [5]. Marked increment of H 2 O 2 and MDA in a dose-dependent manner, as well as EL, observed by Cd stress in the current investigation. ROS degrades the activity of the enzyme that stabilizes lipids and regulates membrane damage. However, the PGRR boosted plants' stress tolerance by retaining water status, uptaking essential nutrients, accumulating metabolites, and augmenting the defensive responses [52]. In a previous report on Cd-spiked B. juncea plants, the bacterial inoculants Serratia sp. decremented the MDA and EL levels by triggering the internal immune system [53]. Moreover, the antioxidant enzyme activities (SOD, CAT, and POD) were uplifted in the G. max with B. cereus inoculation that detoxifies ROS [13]. Similarly, inoculation of Sphingobium yanoikuyae diminished H 2 O 2 and MDA accumulation in the Cd-treated Salix matsudana plants and sustained the membrane structure [54]. Likewise, Chi et al. [14] reported Bacillus sp. inoculation combat the ROS damage and degree of lipid peroxidation by augmenting the antioxidant enzyme activities. However, the P. fungorum and Delftia sp. inoculated rapeseed plants showed reduced Cd-induced oxidative stress indicators (MDA, H 2 O 2 , and EL) that displayed the potential role of endophytes in alleviating oxidative stress in plants.
To dissipate the excess generation of ROS and its damaging effect on the cellular organelles, plants activate their defensive system consisting of several antioxidant enzymes, such as APX, MDHAR, DHAR, GR, GPX, and CAT, thus helping to sustain the stressed period. Moreover, four vital antioxidant enzymes are directly involved in the detoxification of ROS in the AsA-GSH cycle. In the absence of CAT in the chloroplast, APX scavenges ROS along with the GR and maintains redox homeostasis [5]. In the Cd-stressed plants, the APX activity was enhanced, indicating H 2 O 2 scavenging through the production of H 2 O, further accelerating the activity of APX was observed in the bacteria-inoculated plants. The APX-regulated redox homeostasis is initiated with the catalytic reaction in the AsA-GSH cycle, where MDHA is produced and yielded into DHA [55]. Moreover, the APX activity is augmented in the Cd-spiked Medicago sativa with the Rhizobium sp. and Pseudomonas sp. inoculation [17]. Chi et al. [14] reported the enhancement of APX activity with the endophytic inoculation of Bacillus strains in the Cd-stressed S. nigrum plants. Likewise, augmentation of the APX activity was also reported with the Streptomyces sp. and Nocardiopsis sp. inoculated Sorghum bicolor plants under Cd stress [3]. The MDHAR revived the AsA regeneration from MDHA by using NADPH and lowered the ROS-induced cellular damages [9]. In the current study, the endophyte inoculation improved MDHAR activity, increasing the AsA production and thus enhancing stress tolerance. Similarly, the Bacillus inoculants Abelmoschus esculentus plants showed elevated MDHAR activity under drought stress [56]. The DHAR is a GSH-based monomeric enzyme engaged in GSH-dependent DHA recycling [57]. Further regeneration of the GSH from GSSG is mediated by the activity of GR. Higher amounts of GSH help the metal chelation and engage in the MG detoxification system. Under Cd stress, the activities of DHAR and GR were increased, and further acceleration was observed in the bacteria-inoculated plants in the present investigation. Thus, the enhanced activity of antioxidant enzymes in the AsA-GSH cycle enhances ROS scavenging and aids in redox maintenance in the plants [9]. The increased activity of DHAR was reported by Puthiyottil and Akkara [56] in the drought-affected A. esculentus. Moreover, the GR activity was also enhanced in the Cicer arietinum plants upon inoculation with the endophytic B. subtilis under salinity [58]. Other antioxidant enzymes (GPX and CAT) are also engaged in the ROS scavenging system in the plants. The GPX utilized GSH to detoxify H 2 O 2 and convert it to H 2 O. Likewise, CAT detoxifies H 2 O 2 through the production of H 2 O and O 2 . Thus, GPX and CAT play a crucial role in the ROS detoxification system [5]. The activity of GPX was enhanced in the Medicago sativa with the Rhizobium sp. and Pseudomonas sp. inoculation under Cd toxicity [17]. Previously, in the copper-stressed S. lycopersicum, the activity of GPX was also augmented with the B. amyloliquefaciens [59]. Moreover, increased activity of CAT in the S. matsudana with the endophytic S. yanoikuyae is also found under Cd-stressed plants [54]. The activity of GPX and CAT was also augmented in the current study with probiotic bacteria inoculation. Thus, it can be stated that PGPB enhanced the defense responses of plants by participating in the ROS scavenging system and inducing plant tolerance.
Methylglyoxal is a cytotoxic compound produced in plants as a derivative of biochemical pathways under stress. In order to protect the cellular organelles from this glycolytic effect of MG, plants are equipped with an MG detoxification system which consists of Gly I and Gly II enzymes [9]. These enzymes degrade MG and convert it to a non-toxic form in two ways. First, the utilization of GSH by Gly I for the conversion of MG to SLG, and finally, SLG is transformed into D-lactate by the action of Gly II [9]. Under Cd stress, the activities of Gly I and Gly II were reduced remarkably in the current investigation, signifying lesser detoxification of MG-induced oxidative stress in the rapeseed plants. Such declination of glyoxalase enzyme activity was reported in different plants [33,60]. The overproduced MG oxidizes GSH to GSSG and disturbs various physiological pathways [61]. However, the P. fungorum and Delftia sp. showed enhanced activities of Gly I and Gly II in rapeseed plants, which might have upregulated the MG detoxification and improved GSH levels under Cd stress. Moreover, GSH also acts as a metal chelator by producing derivatives of metal complexes and storing them in the vacuole as inert matter [62].
Upon Cd stress, plant growth and reproductive development are significantly affected due to the damaging effects of oxidative stress and nutrient deficiencies, thus causing subsequent yield retardation [63,64]. In this investigation, yield contributing parameters were abruptly affected in the Cd-stressed rapeseed plants, which were correlated with the seed yield plant −1 . The application of endophytic bacteria restored plant physiological attributes, such as P n , T r , and internal carbon dioxide concentration (C i ), which enhanced the plant growth under an abrupt environment and ultimately improved yield parameters [65]. Supplementation of Enterobacter sp. in the Pisum sativum improved 100-seed weight under Cd stress reported by Naveed et al. [63]. In the Cd-stressed P. sativum plants, the bacteria enhanced the P n , T r , and photosynthesis which improved the growth and yield of the plants [63]. Similarly, under Cd stress, the panicle numbers of rice have been increased with the Cd-resistant bacteria B. koreensis. In contrast, the 1000-grain weight was not improved in the bacterial-inoculated plants but reduced the Cd deposition in rice grains [66]. Moreover, Kumar et al. [64] also reported that B. pumilus improved grain weight in salt-stressed rice. Such improvement was attributed to the increment of photosynthetic activities that enhanced carbohydrate translocation and photoassimilate production in the Cd-affected plants with bacterial inoculation. Furthermore, the quality parameters, such as the contents of protein, fat, ash, and fiber of P. sativum, were augmented with the inoculation of Enterobacter sp. under Cd toxicity [63]. Overall, the PGPB inoculation remediates Cd toxicity by improving biochemical and physiological attributes to improve the plant's growth and development, which finally recovered the yield contributing components as well as the yield of rapeseed plants.
In summary, the application of P. fungorum BRRh-4 and Delftia sp. BTL-M2 conferred cadmium tolerance in rapeseed. The Cd-treated plants showed reduced plant growth, biomass accumulation, impaired photosynthetic activity along with reduced RWC. In contrast, increased Pro and indicators of oxidative stress (MDA, H 2 O 2 , and EL) were also observed. However, in the P. fungorum and Delftia sp. inoculated plants, the growth, biomass production, and RWC were increased; in contrast, they decremented the Pro, MDA, H 2 O 2 , and EL levels. The retardation of the oxidative stress indicators correlated with the enhanced activities of antioxidant enzymes, such as APX, MDHAR, DHAR, GR, GPX, and CAT in the bacteria-inoculated plants under Cd toxicity. Another effect of Cd is the accelerated production of MG, which is also mitigated by the augmentation of Gly I and Gly II activities in the Cd-stressed plants. Moreover, it is visible from the current study that the probiotic bacteria P. fungorum and Delftia sp. have the potential to alleviate the Cd stress through modulating ROS scavenging, antioxidant defense system, and glyoxalase system. Therefore, further experimentation should be conducted to explore the molecular mechanisms of the P. fungorum BRRh-4 and Delftia sp. BTL-M2 on the Cd tolerance and the practical application on the contaminated sites to remediate the Cd contamination in an eco-friendly way.

Conclusions
The current study demonstrated the amelioration of the stressful condition of Cd on rapeseed plants' growth, physiology, and biochemical properties through the inoculation of two plant growth-promoting probiotic bacteria, P. fungorum BRRh-4 and Delftia sp. BTL-M2. Under Cd stress, the rapeseed plants showed increased accumulation of ROS, lipid peroxidation, disrupted glyoxalase system, and impaired physiological activities. Upon inoculation with the bacteria in the presence of Cd augmented the antioxidant defense and glyoxalase enzymes, thus mitigating the pernicious effect of ROS and reducing lipid peroxidation in the Cd-stressed plants. Moreover, prevention of pigment damage and improved water status enhanced growth, and biomass accumulation in the bacterial inoculated Cd-stressed plants, which ultimately affected the incrimination of the yield components of rapeseed.