Effects of LED Light Spectra on the Development, Phytochemical Profile, and Antioxidant Activity of Curcuma longa from Easter Island

Curcuma longa (C. longa), an herbaceous plant used for medicinal purposes by the indigenous people of Easter Island, has been overexploited in its natural habitat, leading to its conservation status being designated as a vulnerable species. We have recently reported on the use of light-emitting diodes (LEDs) to improve the productivity of C. longa in vitro cultures under a temporary immersion system (TIS), but the effects of light quality on plant growth, phytochemical composition, and antioxidant capacity remained unexplored. Here, we set out to study these three aspects as observed at the end of TIS culture (day 0) and after 30 days of greenhouse acclimation (day 30). Thus, we evaluated plant morphological characteristics, phytochemical profile (polyphenols, tannins, flavonoids, reducing sugars, and curcumin), and radical scavenging activity by DPPH, ORAC, and FRAP assays. The results showed that, during in vitro cultivation under TIS, the red:blue (RB) LED light spectrum promoted C. longa shoot proliferation, with the resulting seedlings exhibiting greater fresh weight and no signs of etiolation. In the acclimation phase, the RB spectrum increased phytochemicals, such as polyphenols, flavonoids, and reducing sugars, and boosted curcumin synthesis. Nevertheless, the antioxidant activity of the plants under the RB light spectrum did not intensify. We surmise that this may be due to the premature intraplant allocation of metabolites to alternative pathways (e.g., curcumin synthesis) under RB light.


Introduction
Curcuma longa L. (C. longa) is an herbaceous plant widely used in traditional medicine, especially in China and India. In Chile, C. longa is mainly cultivated on Easter Island (also known as Rapa Nui), where it is used to make medicinal extracts to treat cancer, diabetes, muscle pain, etc. A characteristic of the ecotype of C. longa from Rapa Nui is its high concentration of polyphenols and curcumin. Therefore, it has been shown to have attractive biological activity properties, such as antioxidant, antimicrobial, anti-inflammatory, and anticancer activities [1,2]. However, C. longa (leaves and rhizomes) is overexploited, which has led to its conservation status being designated as a vulnerable species.
We recently reported a study on the in vitro micropropagation of C. longa using a temporary immersion system (TIS). In that study, we created experimental culture conditions with the goal of increasing in vitro production of C. longa under TIS. We demonstrated that factors such as the number of explants per flask, flask volume, and light quality all affected the in vitro growth of C. longa. The use of 30 explants per 2 L flask under LEDs with a red:blue ratio of 2:1 resulted in the highest number of seedlings (highest proliferation Plants produced in this study were propagated in vitro using TIS in mainland Chile, prior to shipment to Easter Island. Plants were fully acclimated in a greenhouse prior to shipment [3]. After 45 days of growth and rooting in vitro, C. longa plants were extracted, and their morphological characteristics were analyzed. A total of 284 C. longa plants were obtained from the white light treatment, and 311 from the RB light treatment. Plants from the white light treatment showed elongated leaves, fewer leaves per unit stem length, and larger pale and oxidized areas characteristic of etiolation. In addition, thin and short roots were observed (Figure 1a). In contrast, plants exposed to the RB light spectrum had uniform green pigmentation, less oxidation, and thicker and longer roots.
Furthermore, measurements of plant length and fresh weight at the end of TIS culture ( Figure 2) revealed significant differences in both parameters: plants exposed to white light had greater height, but lower fresh weight compared to RB light-treated plants. Furthermore, measurements of plant length and fresh weight at the end of TIS c ( Figure 2) revealed significant differences in both parameters: plants exposed to light had greater height, but lower fresh weight compared to RB light-treated plan Mean ± S plotted for all replicates analyzed. Tukey's multiple comparison tests were performed at a 95 fidence level in order to compare the differences. **** indicates a significant difference at p < C. longa plants obtained by in vitro propagation using TIS under white and R wavelengths were subsequently acclimatized in a greenhouse under the same resp light spectra. Figure 3 shows the plants in the greenhouse after 30 days of acclimatio morphological differences were observed in plants exposed to the two light qualitie tably, plants exposed to RB light were shorter after TIS culture, but by the end of ac tion, were able to reach the same height as plants exposed to white light. Furthermore, measurements of plant length and fresh weight at the end of TIS c ( Figure 2) revealed significant differences in both parameters: plants exposed to light had greater height, but lower fresh weight compared to RB light-treated plant (a) (b) Figure 2. Effect of light quality on the length and fresh weight of C. longa plants at the end culture (day 0). (a) Plant length; (b) fresh weight per plant. cm: centimeter, g: gram. Mean ± SD plotted for all replicates analyzed. Tukey's multiple comparison tests were performed at a 95 fidence level in order to compare the differences. **** indicates a significant difference at p < C. longa plants obtained by in vitro propagation using TIS under white and RB wavelengths were subsequently acclimatized in a greenhouse under the same resp light spectra. Figure 3 shows the plants in the greenhouse after 30 days of acclimatio morphological differences were observed in plants exposed to the two light qualitie tably, plants exposed to RB light were shorter after TIS culture, but by the end of ac tion, were able to reach the same height as plants exposed to white light. Effect of light quality on the length and fresh weight of C. longa plants at the end of TIS culture (day 0). (a) Plant length; (b) fresh weight per plant. cm: centimeter, g: gram. Mean ± SD were plotted for all replicates analyzed. Tukey's multiple comparison tests were performed at a 95% confidence level in order to compare the differences. **** indicates a significant difference at p < 0.0001.
C. longa plants obtained by in vitro propagation using TIS under white and RB light wavelengths were subsequently acclimatized in a greenhouse under the same respective light spectra. Figure 3 shows the plants in the greenhouse after 30 days of acclimation. No morphological differences were observed in plants exposed to the two light qualities. Notably, plants exposed to RB light were shorter after TIS culture, but by the end of acclimation, were able to reach the same height as plants exposed to white light.

Effects of Light Quality on the Phytochemical Profile of C. longa
Concentrations of phytochemicals such as polyphenols, tannins, flavonoids, redu ing sugars, and curcumin in C. longa plants were determined and compared for both tre ments at the end of in vitro TIS culture (day 0) and at day 30 of subsequent greenhou acclimation ( Figure 4). The analysis showed that the phytochemical profiles of plants e posed to the two treatments were different, with plants under white light treatment i tially having higher mg levels of extract per gram of plant matter compared to the RB lig treatment. However, after 30 days of greenhouse acclimation under the same respecti light spectra and in similar environmental conditions (Figure 4a), the difference disa peared.
At day 0, plants illuminated with white light had half the quantity of polypheno flavonoids, and reducing sugars as plants exposed to RB light. However, at day 30, plan in the white light treatment had significantly greater total tannins, reducing sugars, a curcumin, but fewer polyphenols.
Meanwhile, plants in the RB light treatment exhibited a significant decrease in po phenols, flavonoids, and reducing sugars after 30 days in the greenhouse.
At the end of the TIS culture period, curcumin was not detected in plants grown u der either treatment. However, after 30 days of greenhouse acclimation, both treatmen produced curcumin, with RB light-treated plants having higher levels than plants grow under white light (Figure 4f). At the same time, no statistically significant differences we found with respect to total tannin content ( Figure 4c).

Effects of Light Quality on the Phytochemical Profile of C. longa
Concentrations of phytochemicals such as polyphenols, tannins, flavonoids, reducing sugars, and curcumin in C. longa plants were determined and compared for both treatments at the end of in vitro TIS culture (day 0) and at day 30 of subsequent greenhouse acclimation ( Figure 4). The analysis showed that the phytochemical profiles of plants exposed to the two treatments were different, with plants under white light treatment initially having higher mg levels of extract per gram of plant matter compared to the RB light treatment. However, after 30 days of greenhouse acclimation under the same respective light spectra and in similar environmental conditions (Figure 4a), the difference disappeared.
At day 0, plants illuminated with white light had half the quantity of polyphenols, flavonoids, and reducing sugars as plants exposed to RB light. However, at day 30, plants in the white light treatment had significantly greater total tannins, reducing sugars, and curcumin, but fewer polyphenols.
Meanwhile, plants in the RB light treatment exhibited a significant decrease in polyphenols, flavonoids, and reducing sugars after 30 days in the greenhouse.
At the end of the TIS culture period, curcumin was not detected in plants grown under either treatment. However, after 30 days of greenhouse acclimation, both treatments produced curcumin, with RB light-treated plants having higher levels than plants grown under white light (Figure 4f). At the same time, no statistically significant differences were found with respect to total tannin content ( Figure 4c).

Effect of Light Spectra on C. longa Radical Scavenging Activity
The radical scavenging activity of C. longa plant extracts from the two treatments was determined by three separate methods: free radical scavenging activity of 2,2-diphenyl-1picrylhydrazyl (DPPH), Oxygen Radical Absorbance Capacity (ORAC), and Ferric Reducing Ability of Plasma (FRAP). These are conventional methods for measuring the radical scavenging activity of various substances. Vitamin C (VIT C), Trolox, and gallic acid (GA) were used as positive controls in the DPPH, ORAC, and FRAP assays, respectively.

peared.
At day 0, plants illuminated with white light had half the quantity of polyphenols flavonoids, and reducing sugars as plants exposed to RB light. However, at day 30, plant in the white light treatment had significantly greater total tannins, reducing sugars, an curcumin, but fewer polyphenols. sugars; (f) curcumin. mg: milligrams, g: grams, GAE: gallic acid equivalents, KE: kaempferol equi alents, ND: not detected. Mean ± SD were plotted for all replicates analyzed. Tukey's multiple com parison tests were performed at a 95% confidence level to compare the differences. Bars with diffe ent letters indicate significant differences at p < 0.05 for each parameter studied.

Effect of Light Spectra on C. longa Radical Scavenging Activity
The radical scavenging activity of C. longa plant extracts from the two treatments w determined by three separate methods: free radical scavenging activity of 2,2-diphenylpicrylhydrazyl (DPPH), Oxygen Radical Absorbance Capacity (ORAC), and Ferric Redu ing Ability of Plasma (FRAP). These are conventional methods for measuring the radic scavenging activity of various substances. Vitamin C (VIT C), Trolox, and gallic acid (GA were used as positive controls in the DPPH, ORAC, and FRAP assays, respectively. Figure 5 shows the results of the DPPH, ORAC, and FRAP assays, as well as mea urements of the radical scavenging capacity of the extracts evaluated. At the beginning greenhouse acclimation (day 0), plants exposed to RB light showed higher DPPH sca enging activity compared to plants exposed to white light. After 30 days, the trend w reversed (Figure 5a).
According to the ORAC assay, plants in both treatments initially showed similar rad ical scavenging activity, but after 30 days in the greenhouse, the activity of white ligh (f) curcumin. mg: milligrams, g: grams, GAE: gallic acid equivalents, KE: kaempferol equivalents, ND: not detected. Mean ± SD were plotted for all replicates analyzed. Tukey's multiple comparison tests were performed at a 95% confidence level to compare the differences. Bars with different letters indicate significant differences at p < 0.05 for each parameter studied. Figure 5 shows the results of the DPPH, ORAC, and FRAP assays, as well as measurements of the radical scavenging capacity of the extracts evaluated. At the beginning of greenhouse acclimation (day 0), plants exposed to RB light showed higher DPPH scavenging activity compared to plants exposed to white light. After 30 days, the trend was reversed (Figure 5a).
According to the ORAC assay, plants in both treatments initially showed similar radical scavenging activity, but after 30 days in the greenhouse, the activity of white light-treated plants was slightly higher (Figure 5b).
The largest difference was observed in the FRAP assay, however, where white lighttreated plants showed greater ferric reducing capacity both at the start of acclimation and after 30 days in the greenhouse (Figure 5c). Tukey's multiple comparison tests were performed at a 95% confidence level to compare the differences between treatments, for each parameter analyzed. Bars with different letters indicate significant differences at p < 0.05.

Discussion
We have previously reported experimental conditions for in vitro propagation in TIS culture that would increase production of the Easter Island C. longa ecotype [3]. However, improving the quality of the resulting seedlings would also be very important. Light plays a key role in the processes of photosynthesis and energy conversion in plants. Analysis of the spectral composition of LED luminaires used in this study revealed that the RB and white light spectra differed in their proportions of red, green, blue, and far-red photons ( Table 1). The most significant differences were observed in the percentages of green and red photons: 40.7% and 26.2% in white light compared to 0.5% and 68.4% in RB light, respectively. Furthermore, RB light provided higher daily photosynthetically active irradiance (daily light integral, DLI). These differences affected the growth rate, phytochemical composition, and antioxidant activity of C. longa propagated in vitro.  respectively. Tukey's multiple comparison tests were performed at a 95% confidence level to compare the differences between treatments, for each parameter analyzed. Bars with different letters indicate significant differences at p < 0.05.

Discussion
We have previously reported experimental conditions for in vitro propagation in TIS culture that would increase production of the Easter Island C. longa ecotype [3]. However, improving the quality of the resulting seedlings would also be very important. Light plays a key role in the processes of photosynthesis and energy conversion in plants. Analysis of the spectral composition of LED luminaires used in this study revealed that the RB and white light spectra differed in their proportions of red, green, blue, and far-red photons ( Table 1). The most significant differences were observed in the percentages of green and red photons: 40.7% and 26.2% in white light compared to 0.5% and 68.4% in RB light, respectively. Furthermore, RB light provided higher daily photosynthetically active irradiance (daily light integral, DLI). These differences affected the growth rate, phytochemical composition, and antioxidant activity of C. longa propagated in vitro. We first evaluated in vitro growth of C. longa shoots under two different light spectra. Red and blue wavelengths provide energy to activate photoreceptors and trigger a series of metabolic processes during plant growth, including stem elongation, leaf expansion, stomatal opening, circadian clock, and flowering [15,16]. The RB LED light increased the production of phytochemicals that are used during the growth of C. longa. However, we found that RB LED light promoted the development of more compact seedlings with greater fresh weight, and with leaves without signs of etiolation or chlorosis.
In contrast, seedlings exposed to white light exhibited etiolation, a phenomenon in which morphological changes (elongation of leaves and stems) are activated by the inadequate spectral composition of light [17]. The white light spectrum used in this study had a high proportion of green and far-red photons. This combination has been reported to trigger more shade-induced traits than green or far-red wavelengths alone [18]. In addition, signs of chlorosis (low chlorophyll production) were observed in the leaves, leading to lower synthesis of reducing sugars and other carbohydrates in white light-treated plants [19].
Specific light spectra can alter phytochemical profiles and metabolite accumulation rates in different plant parts (e.g., leaves, rhizomes, and stems). Several authors have shown that certain proportions of red, blue, and green wavelengths in LED luminaires improve growth and development, nutrient levels, and the production and accumulation of bioactive compounds [8,9,20]. Appropriately selected spectral compositions have indirect regulatory effects on gene expression related to metabolite synthesis that help plants adapt to changes in lighting [15].
During in vitro propagation, shoot concentrations of polyphenols, flavonoids, and reducing sugars were found to be higher under RB illumination compared to white light. However, after 30 days in the greenhouse, these analytes decreased in RB light-treated plants, while curcumin levels increased compared to the white light-treated plants. Thus, the proportion of red photons in RB light seems to be the key to gene expression associated with the synthesis of flavonoids and other antioxidants [21,22].
Indeed, similar results were obtained in other species, where exposure to LED lighting with different red:blue ratios increased both antioxidant capacity and polyphenols [23].
Klimek-Szczykutowicz [24] obtained results consistent with the results of the present study when biomass, reducing sugars, and polyphenols, as well as DPPH and FRAP radical scavenging activity, were determined in Nasturtium officinale samples exposed in vitro to LED lighting with different spectral compositions. The concentration of polyphenols and reducing sugars was observed to increase under illumination dominated by blue and red photons [24]. Thus, further studies on the expression of genes related to metabolite production under different light stimuli, as well as its dynamics over time, would be very informative.
Finally, extracts of C. longa from the two treatments were evaluated for their antioxidant activity. Different light spectra can affect the production of reactive oxygen species (ROS), induce oxidative damage, and inhibit the synthesis of secondary metabolites [25]. Studies show that exposure to blue light increases the radical scavenging activity of plant extracts through enhanced synthesis of secondary metabolites, such as anthocyanins, flavonoids, and polyphenols [8,[26][27][28].
Another important observation was the contrasts between the three antioxidant activity assays. White light-treated plants showed higher radical scavenging activity than plants treated with RB light, even though the latter had a higher concentration of polyphenols and accumulated more curcumin. Curcumin is a potent radical scavenger [29], but it accumulated within the rhizomes during the greenhouse acclimation phase and was diluted with components from other parts of the plant during extraction.
In addition, the efficiency of phenolics extraction depends on the method used, as the composition and antioxidant capacity of the obtained components may vary [30]. At the end of TIS culture, RB light-treated plants showed higher antioxidant capacity in DPPH and ORAC assays compared to white light-treated plants. However, after 30 days in the greenhouse, white light-treated seedlings exhibited greater antioxidant capacity in DPPH and FRAP assays, even exceeding that of various positive controls.
The red:blue light treatment in TIS culture increased the synthesis of antioxidant compounds, such as polyphenols and flavonoids, as well as reducing sugars, which became readily available to plants for use in curcumin synthesis during the acclimation phase [31,32]. Premature intraplant allocation of these compounds to alternative metabolic pathways during greenhouse acclimation may be responsible for the reduced antioxidant activity and exaggerated curcumin accumulation in RB light-treated plants.

Explant Collection, Disinfection, and Establishment of Rhizome Cultures
C. longa rhizomes were harvested from the Mataveri Otai nursery on Easter Island (geographical coordinates: 27 • 09 50.17" S-109 • 26 24.63" W). The excised long creeping rhizomes were thoroughly washed with water and Tween-20 for 10 min. The rhizomes were then cut into 2-3 cm pieces and placed under running water for 30 min. Subsequently, the rhizomes were immersed in the Phyton solution (3 mL/L) under gentle agitation for 15 min. Next, the rhizomes were cut into 1 cm pieces, and active buds were separated and washed with 1.5% sodium hypochlorite solution, 0.5 g/L citric acid, and 0.5 g/L ascorbic acid for 15 min under constant agitation. The pieces were transferred to a laminar flow hood and washed four times with a sterile solution of 400 mg/L citric acid and 400 mg/L ascorbic acid. Finally, the pieces were placed in a Murashige and Skoog (MS) culture medium supplemented with 0.1 mg/L thiamine, 200 mg/L glycine, 170 mg/L NaH 2 PO 4 , 2.5 mL/L plant preservative mixture (PPM), 6.5 g/L agar, and 30 g/L sucrose at pH 5.8. The flasks were incubated in vitro in the dark at 25 • C for 10 days, and then placed under 1R/1B LED light (50% red (peak at 660 nm) + 50% blue (420-460 nm), PARALED system, Ciencia Pura SpA, Santiago, Chile) for one month.

In Vitro Growth of Explants
The shoot rhizomes were transferred to the MS growth medium, supplemented with 0.1 mg/L thiamine, 200 mg/L glycine, 170 mg/L NaH 2 PO 4 , 1 mg/L 1-Naphthaleneacetic acid (NAA), 2 mg/L N 6 -Benzylaminopurine (BAP), 6.5 g/L agar, and 30 g/L sucrose at pH 5.8. The plant material was subcultured three times (30 days each time) to obtain more shoots for TIS. The initial explants used for TIS were 1-2 cm long, rootless, and showed no symptoms of vitrification (hyperhydricity) or oxidation.

In Vitro Proliferation of Curcuma longa by TIS
A temporary immersion system was used for the proliferation and rooting of C. longa shoots. Proliferation was performed in an MS culture medium, supplemented with 200 mg/L glycine, 0.1 mg/L thiamine, 0.5 mg/L nicotinic acid, 0.5 mg/L pyridoxine, 100 mg/L myoinositol, 170 mg/L NaH 2 PO 4 , 3 mg/L BAP, and 30 g/L sucrose at pH 5.8. For the rooting stage, the same medium was used, and BAP was replaced with 1 mg/L NAA. Twenty-one flasks for each set of conditions were used. Flasks were maintained under the following conditions during proliferation and rooting: 30 explants and 450 mL of culture medium (proliferation or rooting) per flask, immersion of 4 min each time in a 4 h cycle, and a temperature of 23 ± 1 • C for 16 h/day. After the proliferation stage, the flask of the proliferation medium was replaced with the flask of the rooting medium under a laminar flow hood, while the explants were not disturbed by handling. After both stages, all shoots were placed in a greenhouse to acclimate. The light and environmental conditions were the same for all the conditions studied.

Spectral Light Conditions
To explore the effects of LED light on the growth and phytochemical composition of C. longa, two treatments were set up as follows: (i) white LED light as control; and (ii) RB LED light (PARALED system, Ciencia Pura SpA, Santiago, Chile). Photon fluxes were measured using a compact spectrophotometer (Lighting Passport Standard Pro model, Allied Scientific ProTM, Gatineau, QC, Canada). To avoid light contamination, all assays were separated from each other by white screens made by a local manufacturer. The characteristics of experimental illumination are shown in Table 1.

Phytochemical Composition
Samples of C. longa plants were collected at the end of TIS culture and on day 30 after the initiation of acclimation. Samples were processed and analyzed to determine the amounts of the following metabolites: polyphenols, tannins, flavonoids, reducing sugars, and curcumin. Samples were dried at 40 • C for two weeks, and lipids were extracted using hexane solvent with a Soxhlet system.

Polyphenols
To obtain polyphenol-rich extracts, dry samples were mixed with 95% ethanol under agitation for 48 h. The ethanol was removed with a rotary evaporator and the extracts were resuspended in DMSO. A modified Folin-Ciocalteu method was used to quantify the total content of polyphenols [33]. The results were expressed in mg gallic acid equivalent (GAE)/g extract.

Total Tannins
A modified Folin-Ciocalteu method was used for the quantification of tannins [33]. Briefly, 100 µL extract and 500 µL water were mixed with 250 µL Folin-Ciocalteu 1N reagent under vigorous shaking. Then, 35% Na 2 CO 3 was added, and the sample was shaken again for 30 min. The absorbance was measured at 725 nm and the data were interpolated on the gallic acid calibration curve. The results were expressed in mg gallic acid equivalent (GAE)/g extract.

Flavonoids
Flavonoids were determined by the method of Liu [34], with modifications. The reaction mixture contained 30 µL sodium nitrite (10% w/v), 60 µL aluminum chloride hexahydrate (20% w/v), 200 µL NaOH (1M), 400 µL distilled water, and a 100 µL sample for analysis. The absorbance was measured after 5 min of reaction at 415 nm, and the values were interpolated on a kaempferol calibration curve. The results were expressed as mg kaempferol (KE) per gram of dry extract. The experiments were performed in triplicate.

Reducing Sugars
Reducing sugars were quantified by the modified DNS method [35], whereby 100 µL extract and 1000 µL DNS reagent were mixed with vortex; the mixture was heated in boiling water for 5 min; then, 8 mL water was added, and the absorbance was measured at 540 nm. The results were expressed in mg glucose/L.

Determination of Curcumin
A calibration curve of 1 to 25 µg/mL curcumin was made, and the absorbance was measured at 421 nm. Dilutions of the original extract were made and measured at 421 nm, according to the modified protocol of Hazra et al. [36]. The radical scavenging activity of the extracts was evaluated by the DPPH (1,1diphenyl-2-picrylhydrazyl) assay [37]. Briefly, 1 mL of 0.1 mM DPPH radical solution in ethanol was mixed with 50 µL leaf, rhizome extracts, gallic acid, or vitamin C at a concentration of 20 µg/mL. The antioxidant activity of the extracts was evaluated by the DPPH assay. DPPH was reduced and the color change was measured at 518 nm after 20 min of reaction time using an Epoch ELISA reader (ELx800, BioTek ® , Winooski, VT, USA). The percentage of DPPH inhibition was calculated using the following equation: % Inhibition = Abs control − Abs sample Abs control × 100 (1) where "Abs control" is the absorbance of DPPH in the absence of a sample, and "Abs sample" is the absorbance of DPPH in the presence of either a sample or the standard (gallic acid). The experiments were performed in triplicate.

ORAC-FL Assay
The ORAC value was measured according to the method described by Ou [38], with modifications [39]. The reaction was conducted in sodium phosphate buffer (75 mM, pH 7.4) using black-walled 96-well plates with a final volume of 200 µL. Twenty µL each of extract or Trolox (20 µg/mL) and fluorescein solutions (120 µL; 70 nM, final concentration) were placed in each microplate well. The mixture was pre-incubated for 15 min at 37 • C. The AAPH solution (60 µL; 12 mM final concentration) was added rapidly, and the microplate was read immediately with a fluorescence reader (Synergy HT multi-detection microplate reader; BioTek ® Instruments, Inc., Winooski, VT, USA). Fluorescence was recorded every minute for 80 min from normalized curves, and the area under the fluorescence decay curve (AUC) was calculated as follows: where f 0 is the initial fluorescence reading at 0 min and f i is the fluorescence reading at time i. The AUC of a sample was calculated by subtracting the AUC of the blank space. Regression equations between net AUC and antioxidant concentration were calculated for all samples. ORAC-FL values were expressed as Trolox equivalents using the standard curve calculated for each assay. Results were expressed in µmol of Trolox equivalent/µmol of extract. The experiments were performed in triplicate.

FRAP Assay
The iron reducing power of each fraction was determined as described by Dudonné [40], with modifications. A functional FRAP reagent was prepared freshly by mixing 10 volumes of 300 mM acetate buffer, pH 3.6, 1.0 volume of 10 mM TPTZ (2,4,6-tri(2-pyridyl)-s-triazine) in 40 mM hydrochloric acid, and 1.0 volume of 20 mM ferric chloride; 100 µL sample solution (1 mg/mL) and 300 µL deionized water were added to 3 mL of the freshly prepared FRAP reagent. The reaction mixture was incubated for 30 min at 37 • C in a water bath. The absorbance of the samples was then measured at 593 nm, and FRAP values were expressed as mmol Fe 2+ /g sample. All measurements were performed in triplicate.

Statistical Analysis
A two-way ANOVA, along with Tukey's multiple comparison tests, was used for comparisons; p < 0.05 was considered a statistically significant difference. All statistical analyses were calculated using the GraphPad Prism 9 computer software (GraphPad Software, San Diego, CA, USA).

Conclusions
Red:blue LED light enhanced the proliferation of C. longa shoots in vitro by TIS. In addition, the resulting plantlets had greater fresh weight and no signs of etiolation. Plants under RB light also exhibited higher levels of phytochemicals such as polyphenols, flavonoids, and reducing sugars, which stimulated curcumin synthesis in the acclimation phase. Nevertheless, RB light-treated plants did not exhibit better antioxidant activity, possibly due to the premature intraplant allocation of metabolites to alternative pathways, such as curcumin