Immunomodulatory Effects of Plant Extracts from Salvia deserta Schang. and Salvia sclarea L.

Medicines, their safety, effectiveness and quality are indispensable factors of national security, important on a global scale. The COVID-19 pandemic has once again emphasized the importance of improving the immune response of the body in the face of severe viral infections. Plants from the Salvia L. genus have long been used in traditional medicine for treatment of inflammatory processes, parasitic diseases, bacterial and viral infections. The aim of the current study was to evaluate the immunomodulatory effects of plant extracts LS-1, LS-2 from Salvia deserta Schang. and LS-3, LS-4 from Salvia sclarea L. plants growing in southern Kazakhstan by conventional and ultrasonic-assisted extraction, respectively. The cytotoxic effects of the named sage extracts on neonatal human dermal fibroblasts (HDFn) were evaluated using the MTT assay. Immunomodulatory effects of the studied extracts were compared by examining their influence on pro-inflammatory cytokine secretion and phagocytic activity of murine immune cells. Depending on the physiological state of the innate immune cells, sage extracts LS-2 and LS-3 had either a stimulating effect on inactivated macrophages or suppressed cytokine-producing activity in LPS-activated macrophages. The greatest increase in TNF-α secretion was found after treatment of spleen T lymphocytes with sage extract LS-2, obtained by ultrasonic-assisted extraction.


Introduction
The importance of the quality of medicinal products for national security was once again highlighted at the 74th World Health Assembly in 2021, with improved and more equitable access to health products designated as a global priority. Herbal drugs, due to their similarity with the physiological systems of the body, are capable of purposefully inducing and mobilizing its protective resources. The advantage of herbal drugs is in the mildness and complexity of their therapeutic action, low toxicity, lack of cumulative effect, addiction, and rare induction of allergic reactions, which is especially important in the case of diseases requiring long-term treatment. For some diseases, herbal drugs may be the only cure. Subsequently, a clear tendency towards an increase in the share of herbal drugs in the total amount of manufactured medicines in leading world countries can obviously be traced, and this figure has now exceeded 50% [1].
It is known from the Sixth National Report of the Republic of Kazakhstan (RK) on biological diversity that the wild-growing flora of the country are represented by 5754 species of higher vascular plants and up to 14% of endemic plants. More than 700 plant species

Testing the Quality of Raw Materials
To obtain quality products, it is important to use quality material. In addition to general identification and visual evaluation, assessment of numerical indicators is significant. In particular, ash, insoluble in 10% HCl, is represented mainly by SiO 2 and characterizes the contamination of raw materials with mineral impurities. The studied plants comply with the basic requirements for medicinal plant raw material by the State Pharmacopeia of the Republic of Kazakhstan (SP RK) [3,[33][34][35], Table 1. The numerical indicators of plant quality, as well as the indicators of their microbiological purity and radionuclide control (established commercially at the Testing Center of JSC "Scientific Center for Anti-Infectious Drugs"), testify to their quality and, consequently, to the possibility of getting valuable extracts on their basis.
When evaluating and standardizing medicinal plant raw material, wherever possible, quantitative analysis of biologically active and/or extractive compounds is also included [3,[33][34][35]. The phytochemical analysis (Table 2) showed the presence of hydrophobic compounds, amino acids, polysaccharides, and a significant number of phenolic compounds, particularly flavonoids, tannins and phenolic acids.

Preparation of the Extracts
The choice of the optimal technology for isolation of an extract from a studied plant material included a number of stages and provided for its most complete depletion in order to maximize the efficiency of obtaining the desired complex of valuable constituents. For this, the selection of the most suitable concentration of the extragent, its optimal ratio with the raw material, as well as the duration, temperature and frequency of extraction were elaborated. Two methods were used for isolation of extracts: conventional extraction and ultrasonic-assisted extraction.
It was found that the maximum yield of extract LS-1 from S. deserta Schang. by the method of conventional extraction is achieved when it is extracted with 50% ethanol, with the ratio of the selected extragent to raw material equal to 1:10 ( Figure 1).
To find the best concentration of the extragent for obtaining the LS-1 extract, 50 g of selected plant raw material was extracted using aqueous solutions of 30%, 50% and 70% ethanol as extragent at a temperature of 30 • C for 60 min ( Figure 2).
As can be seen from the averaged data presented on Figure 2, a higher yield of LS-1 extract is achieved when the raw material is extracted with 50% ethanol. The same is true for LS-3. To find the best concentration of the extragent for obtaining the LS-1 extract, 50 g of selected plant raw material was extracted using aqueous solutions of 30%, 50% and 70% ethanol as extragent at a temperature of 30 °C for 60 min (Figure 2). As can be seen from the averaged data presented on Figure 2, a higher yield of LS-1 extract is achieved when the raw material is extracted with 50% ethanol. The same is true for LS-3.
To establish the optimal time for ultrasonic-assisted extraction, the duration of the extraction process was changed from 30 to 90 min at an optimal temperature of 30 °C, with the ratio of raw material to extragent equal to 1:10 ( Figure 3). As can be seen from the data presented on Figure 3, the maximum yield of the extract LS-2 is reached within 60 min. Thus, by varying the parameters of ultrasonic-assisted extraction in triplicate, optimal conditions for obtaining the extract LS-2 were elaborated: extraction of raw material with 50% ethanol at a ratio of raw material to extragent equal to 1:10 at a temperature of 30 °C for 60 min. The same conditions were proven to work well for obtaining the extract LS-4.  To find the best concentration of the extragent for obtaining the LS-1 extract, 50 g of selected plant raw material was extracted using aqueous solutions of 30%, 50% and 70% ethanol as extragent at a temperature of 30 °C for 60 min (Figure 2). As can be seen from the averaged data presented on Figure 2, a higher yield of LS-1 extract is achieved when the raw material is extracted with 50% ethanol. The same is true for LS-3.
To establish the optimal time for ultrasonic-assisted extraction, the duration of the extraction process was changed from 30 to 90 min at an optimal temperature of 30 °C, with the ratio of raw material to extragent equal to 1:10 ( Figure 3). As can be seen from the data presented on Figure 3, the maximum yield of the extract LS-2 is reached within 60 min. Thus, by varying the parameters of ultrasonic-assisted extraction in triplicate, optimal conditions for obtaining the extract LS-2 were elaborated: extraction of raw material with 50% ethanol at a ratio of raw material to extragent equal to 1:10 at a temperature of 30 °C for 60 min. The same conditions were proven to work well for obtaining the extract LS-4. To establish the optimal time for ultrasonic-assisted extraction, the duration of the extraction process was changed from 30 to 90 min at an optimal temperature of 30 • C, with the ratio of raw material to extragent equal to 1:10 ( Figure 3). To find the best concentration of the extragent for obtaining the LS-1 extract, 50 g of selected plant raw material was extracted using aqueous solutions of 30%, 50% and 70% ethanol as extragent at a temperature of 30 °C for 60 min (Figure 2). As can be seen from the averaged data presented on Figure 2, a higher yield of LS-1 extract is achieved when the raw material is extracted with 50% ethanol. The same is true for LS-3.
To establish the optimal time for ultrasonic-assisted extraction, the duration of the extraction process was changed from 30 to 90 min at an optimal temperature of 30 °C, with the ratio of raw material to extragent equal to 1:10 ( Figure 3). As can be seen from the data presented on Figure 3, the maximum yield of the extract LS-2 is reached within 60 min. Thus, by varying the parameters of ultrasonic-assisted extraction in triplicate, optimal conditions for obtaining the extract LS-2 were elaborated: extraction of raw material with 50% ethanol at a ratio of raw material to extragent equal to 1:10 at a temperature of 30 °C for 60 min. The same conditions were proven to work well for obtaining the extract LS-4. As can be seen from the data presented on Figure 3, the maximum yield of the extract LS-2 is reached within 60 min. Thus, by varying the parameters of ultrasonic-assisted extraction in triplicate, optimal conditions for obtaining the extract LS-2 were elaborated: extraction of raw material with 50% ethanol at a ratio of raw material to extragent equal to 1:10 at a temperature of 30 • C for 60 min. The same conditions were proven to work well for obtaining the extract LS-4.
The resulting extracts are crystalline with a bitter, astringent taste, with a weak specific odor, formed of anisodiametric crystals in the form of plates, with complex ununiformed surfaces. They are insoluble in hydrophobic solvents and soluble in hydrophilic solventsdimethyl sulfoxide, dimethylformamide, in aqueous solutions of ethanol and acetone (30% and 50%), and moderately soluble in water at 25 • C. Like all plant extracts, they are characterized by low flowability due to their strong hygroscopicity.
The results of a comparative analysis of the quantitative content of main BAC groups in extracts LS-1 and LS-2, as well as LS-3 and LS-4 are presented in Tables 3 and 4.

Cell Viability
Cytotoxic effects of sage extracts of S. deserta Schang. (LS-1 and LS-2) and S. sclarea L. (LS-3 and LS-4), obtained by conventional extraction and ultrasonic-assisted extraction, on neonatal human dermal fibroblasts (HDFn) were evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The results of the MTT assay ( Figure 5) showed that all the obtained sage extracts from S. deserta Schang. ( Figure 5A) and S. sclarea L. ( Figure 5B) do not affect the cell viability of HDFn at different concentrations. Cell viability after incubation with sage extracts at a concentration of 50 mg/mL was more than 70% with median cellular cytotoxic concentrations (CC50) values > 50 mg/mL, officially accepted, which indicates that even at a higher concentration, sage extracts do not cause a significant toxic effect on human fibroblasts. In addition, notable differences between sage extracts obtained by conventional (LS-1, LS-3) and ultrasonic-assisted extraction (LS-2, LS-4) were not found.

Immunomodulatory Effects of Sage Extracts
In this study we compared immunomodulatory effects of the extracts of S. deserta Schang. (LS-1 and LS-2) and S. sclarea L. (LS-3 and LS-4) obtained by conventional and ultrasonic-assisted extraction on pro-inflammatory cytokine secretion and phagocytic activity of murine immune cells.
To compare the immunomodulatory activity of sage extracts obtained by two different extraction methods, commercial ELISA kits were used to determine the levels of proinflammatory cytokine secretion (TNF-α and IL-1β) by murine peritoneal macrophages and spleen T lymphocytes. Two controls were used in the experiment: macrophages/T-lymphocytes without treatment and macrophages/T lymphocytes activated by lipopolysaccharide (LPS) or concanavalin A (ConA), respectively. Cell viability after incubation with sage extracts at a concentration of 50 mg/mL was more than 70% with median cellular cytotoxic concentrations (CC50) values > 50 mg/mL, officially accepted, which indicates that even at a higher concentration, sage extracts do not cause a significant toxic effect on human fibroblasts. In addition, notable differences between sage extracts obtained by conventional (LS-1, LS-3) and ultrasonic-assisted extraction (LS-2, LS-4) were not found.

Immunomodulatory Effects of Sage Extracts
In this study we compared immunomodulatory effects of the extracts of S. deserta Schang. (LS-1 and LS-2) and S. sclarea L. (LS-3 and LS-4) obtained by conventional and ultrasonic-assisted extraction on pro-inflammatory cytokine secretion and phagocytic activity of murine immune cells.
To compare the immunomodulatory activity of sage extracts obtained by two different extraction methods, commercial ELISA kits were used to determine the levels of proinflammatory cytokine secretion (TNF-α and IL-1β) by murine peritoneal macrophages and spleen T lymphocytes. Two controls were used in the experiment: macrophages/T-lymphocytes without treatment and macrophages/T lymphocytes activated by lipopolysaccharide (LPS) or concanavalin A (ConA), respectively.
In the wells with macrophages and T lymphocytes, 50 mg/mL of plant extracts were added and incubated for 24 h at 37% and 5% CO 2 . ELISA showed that extracts of S. deserta Schang. (LS-1 and LS-2) significantly suppressed the level of TNF-α production in both intact and LPS-activated macrophages ( Figure 6). At the same time, no significant difference was found between the effects of extracts LS-1 (conventional extraction) and LS-2 (ultrasonic-assisted extraction). ference was found between the effects of extracts LS-1 (conventional extraction) and LS-2 (ultrasonic-assisted extraction).
Measurement of IL-1β levels in intact macrophages showed that sage extract obtained by ultrasonic-assisted extraction (LS-2) caused a significant increase in IL-1β level compared to the control and LS-1 sage extract. In contrast, the level of IL-1β production in LPS-activated macrophages was significantly suppressed after incubation with LS-2 sage extract. The study of the effect of sage extracts of S. deserta Schang. on TNF-α production by spleen T lymphocytes showed that sage extracts induced a significant increase in TNF-producing activity in both intact and Con A-activated T lymphocytes ( Figure 7). The greatest increase in TNF-α secretion was found after treatment of spleen T lymphocytes with sage extract (LS-2) obtained by ultrasonic-assisted extraction. Measurement of IL-1β levels in intact macrophages showed that sage extract obtained by ultrasonic-assisted extraction (LS-2) caused a significant increase in IL-1β level compared to the control and LS-1 sage extract. In contrast, the level of IL-1β production in LPSactivated macrophages was significantly suppressed after incubation with LS-2 sage extract.
The study of the effect of sage extracts of S. deserta Schang. on TNF-α production by spleen T lymphocytes showed that sage extracts induced a significant increase in TNFproducing activity in both intact and Con A-activated T lymphocytes ( Figure 7). The greatest increase in TNF-α secretion was found after treatment of spleen T lymphocytes with sage extract (LS-2) obtained by ultrasonic-assisted extraction. The same ELISA study was conducted for extracts from S. sclarea L. Comparative analysis of the immunomodulatory activity of S. sclarea L. extracts obtained by conventional extraction (LS-3) and ultrasonic-assisted extraction (LS-4) showed that both sage extracts significantly suppressed the level of TNF-α production in both intact and The same ELISA study was conducted for extracts from S. sclarea L. Comparative analysis of the immunomodulatory activity of S. sclarea L. extracts obtained by conventional extraction (LS-3) and ultrasonic-assisted extraction (LS-4) showed that both sage extracts significantly suppressed the level of TNF-α production in both intact and LPS-activated macrophages ( Figure 8).

Figure 7.
Effects of S. deserta Schang. extracts on the secretion of TNF-α by spleen T lymphocytes. (LS-1)-sage extract obtained by conventional extraction and (LS-2)-sage extract obtained by ultrasonic-assisted extraction. Two controls were used in the experiment: spleen T lymphocytes without treatment or stimulus and spleen T lymphocytes activated with Con A. (*) indicates significant differences in comparison to control, p < 0.05.
The same ELISA study was conducted for extracts from S. sclarea L. Comparative analysis of the immunomodulatory activity of S. sclarea L. extracts obtained by conventional extraction (LS-3) and ultrasonic-assisted extraction (LS-4) showed that both sage extracts significantly suppressed the level of TNF-α production in both intact and LPS-activated macrophages (Figure 8). ELISA has revealed that the treatment of macrophages with S. sclarea L. extracts has a different effect on the level of IL-1β production by macrophages, for instance, with secretion of IL-1β in the intact macrophages after treatment with S. sclarea L. extracts, especially LS-3. On the other hand, S. sclarea L. extracts suppress the production of this pro-inflammatory cytokine by LPS-activated macrophages.
The analysis of TNF-producing activity of spleen T lymphocytes demonstrated that S. sclarea L. extracts resulted in increase in the level of the production in both intact and Con A-activated T lymphocytes ( Figure 9). ELISA has revealed that the treatment of macrophages with S. sclarea L. extracts has a different effect on the level of IL-1β production by macrophages, for instance, with secretion of IL-1β in the intact macrophages after treatment with S. sclarea L. extracts, especially LS-3. On the other hand, S. sclarea L. extracts suppress the production of this pro-inflammatory cytokine by LPS-activated macrophages.
The analysis of TNF-producing activity of spleen T lymphocytes demonstrated that S. sclarea L. extracts resulted in increase in the level of the production in both intact and Con A-activated T lymphocytes (Figure 9). Besides analysis of the cytokine-producing activity of immune cells, the phagocytic activity of peritoneal macrophages has been also studied after treatment with extracts of S. deserta Schang. and S. sclarea L.
The results of a comparative analysis of the effects of sage extracts showed that, regardless of the type of sage extract and the extraction method, all sage extracts significantly increase the phagocytic activity of peritoneal macrophages (Figure 10). Besides analysis of the cytokine-producing activity of immune cells, the phagocytic activity of peritoneal macrophages has been also studied after treatment with extracts of S. deserta Schang. and S. sclarea L.
The results of a comparative analysis of the effects of sage extracts showed that, regardless of the type of sage extract and the extraction method, all sage extracts significantly increase the phagocytic activity of peritoneal macrophages ( Figure 10).
(LS-3)-sage extract obtained by conventional extraction and (LS-4)-sage extract obtained by ul-trasonic-assisted extraction. Two controls were used in the experiment: spleen T lymphocytes without treatment or stimulus and spleen T lymphocytes activated with Con A. (*) indicates significant differences in comparison to control, p < 0.05.
Besides analysis of the cytokine-producing activity of immune cells, the phagocytic activity of peritoneal macrophages has been also studied after treatment with extracts of S. deserta Schang. and S. sclarea L.
The results of a comparative analysis of the effects of sage extracts showed that, regardless of the type of sage extract and the extraction method, all sage extracts significantly increase the phagocytic activity of peritoneal macrophages ( Figure 10). There was no significant difference in macrophage phagocytic activity between the studied extracts.

Discussion
It is well known that medicinal plants are a source of a large number of biologically active compounds (polyphenols, flavonoids, polysaccharides, alkaloids, saponins, terpenes, lipids, etc.) capable of modulating the cellular and humoral immune responses [36,37]. Immunomodulatory potential of such can be used for treatment of immune-mediated inflammatory diseases, infections and tumors [38,39].
At present, there is a number of studies on the immunomodulatory effects of different Salvia species. As such, it has been reported that the extracts of S. miltiorrhiza sig- There was no significant difference in macrophage phagocytic activity between the studied extracts.

Discussion
It is well known that medicinal plants are a source of a large number of biologically active compounds (polyphenols, flavonoids, polysaccharides, alkaloids, saponins, terpenes, lipids, etc.) capable of modulating the cellular and humoral immune responses [36,37]. Immunomodulatory potential of such can be used for treatment of immune-mediated inflammatory diseases, infections and tumors [38,39].
At present, there is a number of studies on the immunomodulatory effects of different Salvia species. As such, it has been reported that the extracts of S. miltiorrhiza significantly enhanced cell-mediated immunity in healthy subjects and breast cancer patients [40,41]. Moon et al. have demonstrated that extracts of S. miltiorrhiza inhibited the production of pro-inflammatory cytokines via down-regulation of gene and protein expression, whereas it increased the anti-inflammatory cytokines in LPS-induced macrophages [42]. Other researchers revealed that different tanshinones from S. miltiorrhiza markedly inhibited production of IL-12 in activated macrophages and antigen-presenting cells [43]. Shin et al. showed immunostimulatory effects of S. plebeia R. aqueous extract significantly increased phagocytic activities, nitric oxide (NO) production, and pro-inflammatory cytokines TNF-α and IL-1β in Raw264.7 macrophages [44].
In our study, we examined the effects of the extracts of S. deserta Schang. and S. sclarea L. obtained by conventional and ultrasonic-assisted extraction on the production of proinflammatory cytokines and phagocytic activity of murine immune cells. Before the investigation of the immunomodulatory effects of sage extracts, we first evaluated the cytotoxicity of different concentration of the extracts of S. deserta Schang. and S. sclarea L. (0.098∼50 mg/mL) in dermal fibroblasts. The results of the cytotoxicity assay indicated that the tested concentrations of sage extracts did not affect cell viability.
In order to study the immunomodulatory effects of sage extracts, we conducted ELISA for measurement of TNF-α and IL-1β in murine peritoneal macrophages and splenic T lymphocytes with and without stimulation by LPS or Con A. LPS and Con A were used as immunogenic stimulators to produce cytokines by macrophages and T lymphocytes, respectively. TNF-α and IL-1β are mainly produced by macrophages and monocytes and are considered the most important cytokines that promote inflammatory responses [45].
Our results showed that the extracts of S. deserta Schang. and S. sclarea L. obtained by conventional and ultrasonic-assisted extraction significantly reduced secretion of proinflammatory cytokine TNF-α in both unstimulated and LPS-stimulated peritoneal macrophages. However, interesting data have been obtained when measuring IL-1β levels in macrophages. When unstimulated macrophages were treated with S. deserta Schang. extract (LS-2) and S. sclarea L. extract (LS-3) the release of IL-1β in the macrophages was significantly increased in comparison to control. On the other hand, when LPS-stimulated macrophages were treated with the same sage extracts, the level of IL-1β secretion in macrophages was significantly inhibited.
The data obtained by us are in good agreement with the data obtained by other scientists, who showed that several groups of plant secondary metabolites impact the functional activity of macrophages, including phenolic compounds, terpenes, and polysaccharides [46]. For instance, it has been reported that the extract of S. miltiorrhiza inhibits the production of nitric oxide, PGE2, IL-1β, IL-6, and TNF-α, and the expression of chemokines, RANTES, CX3CL1, CX3CR1, as well as inflammatory mediators such as TLR-4 and 11β-HSD1 in LPS-stimulated RAW 264.7 macrophages [43]. On the contrary, Im et al. found that polysaccharides isolated from Salicornia herbacea stimulate intact murine macrophages to release high levels of cytokines TNF-α, IL-1β and nitric oxide [47]. Other researchers demonstrated that pretreatment with S. miltiorrhiza polysaccharides lead to a significant reduction of TNF-α, IL-6 and IL-1β in LPS-stimulated macrophages [48].
Several studies have shown that plant polysaccharides can regulate phagocytosis of macrophages [49][50][51]. Phagocytosis is an essential element for host defense which combines bacterial elimination with initiation of the innate immune response and antigen presentation to T lymphocytes. In our study we revealed that treatment of intact peritoneal macrophages with examined plant extracts significantly increased the phagocytic activity of macrophages in comparison to a control. We suggest that the immunostimulatory effects observed in our study are associated with sage polysaccharides, which may bind to mannose receptors on macrophages and activate their phagocytic activity, production of reactive oxygen species and the secretion of cytokines.
T lymphocytes are the major cell population of the adaptive immune system. They play key roles in elimination of infected and foreign cells, activation of other immune cells, secretion of cytokines and modulation of the immune response [52]. Recently, Chen et al. demonstrated that S. miltiorrhiza polysaccharide can specifically promote the proliferation and enhance cytotoxicity of T lymphocytes in peripheral blood of cancer patients through the activation of TLRs, MAPK and NF-κB signaling pathways [53]. In our study, we have also found that extracts of S. deserta Schang. (LS-2) and S. sclarea L. (LS-3) significantly increased secretion of TNF-α by intact and Con A-activated splenic T lymphocytes. The greatest immunostimulant effect was found in T lymphocytes treated with sage extracts LS-2 and LS-3.
Thus, based on our results and literature data, we suggest that extracts of S. deserta Schang. (LS-2) and S. sclarea L. (LS-3) contain polysaccharides with remarkable biological properties able to modulate the functional activity of macrophages and T lymphocytes depending on their physiological state, and might be used for further preclinical and clinical studies.

Checking the Quality of Plant Materials and Obtaining the Extracts
The harvesting of two investigated species of plants of the genus sage was carried out during their flowering period, June 2021. During the trip to the territory of the Kordai district of the Zhambyl region (the total length of the route: Almaty-Otar-Kenen-UlkenSulutor-Kenen-Otar-Almaty was 400 km) the coordinates of the place of collection and growth of the species S. sclarea L., S. deserta Schang. are marked ( Table 5), photos of the objects of the study were taken (Figure 11), and raw material was collected for study and production of extracts.  After the collection and initial treatment of plant materials (cleaning, drying and crushing) numerical indicators were determined to test their quality using the methods of SP RK in triplicate, with average results presented in Table 1 [33][34][35]. In addition, indicators of their microbiological purity and radionuclide have been tested commercially in a certified laboratory of the of JSC "Scientific Center for Anti-Infectious Drugs" Testing Center.

Preparation of Extracts
To elaborate the optimal conditions for obtaining the extracts, 30%, 50%, and 70% ethanol solutions were used with the choice of the most suitable concentration, providing a more complete depletion of raw materials; the ratio of the selected extragent to raw material was established with a change in their ratio in the generally accepted range for the aerial part of medicinal plants from 1:5 to 1:10, the extraction time (with a change in it in the range from 5 to 48 h) and the extraction rate (when its number changed from one to three). Dry extracts were obtained using a rotary evaporator model IKA RV20 (IKA ® -Werke GmbH & Co. KG, Staufen, (Germany). The mode of ultrasonic waves was changed in the range of 30-40 kHz, the power 300-1200 W, the time of ultrasound exposure was 10-60 min, and the temperature was 30-60 °C [54,55]. The parameter of the optimization of the extraction process was the yield of extracts and the quantitative content of tannins and polysaccharides in it.
For chromatographic control of the studied objects, we used the methods of one-dimensional and two-dimensional chromatography on paper and thin layer chromatography using standard samples, specific developers, diagnosing and complexing reagents. Chromatography on FN3 paper (Sartorius Lab Instruments GmbH & Co. KG, Goettingen, Germany) was carried out in solvent systems (ratio by volume): n-butanol-acetic acid-water (40:12.5:29); n-butanol-acetic acid-water (4:1:5); ben- After the collection and initial treatment of plant materials (cleaning, drying and crushing) numerical indicators were determined to test their quality using the methods of SP RK in triplicate, with average results presented in Table 1 [33][34][35]. In addition, indicators of their microbiological purity and radionuclide have been tested commercially in a certified laboratory of the of JSC "Scientific Center for Anti-Infectious Drugs" Testing Center.

Preparation of Extracts
To elaborate the optimal conditions for obtaining the extracts, 30%, 50%, and 70% ethanol solutions were used with the choice of the most suitable concentration, providing a more complete depletion of raw materials; the ratio of the selected extragent to raw material was established with a change in their ratio in the generally accepted range for the aerial part of medicinal plants from 1:5 to 1:10, the extraction time (with a change in it in the range from 5 to 48 h) and the extraction rate (when its number changed from one to three). Dry extracts were obtained using a rotary evaporator model IKA RV20 (IKA ® -Werke GmbH & Co. KG, Staufen, (Germany). The mode of ultrasonic waves was changed in the range of 30-40 kHz, the power 300-1200 W, the time of ultrasound exposure was 10-60 min, and the temperature was 30-60 • C [54,55]. The parameter of the optimization of the extraction process was the yield of extracts and the quantitative content of tannins and polysaccharides in it.
All high-purity chemicals were obtained from Sigma-Aldrich (St. Louis, MA, USA). All solutions were prepared with ddH 2 O.
The quantitative content of tannins in extracts was determined spectrophotometrically using a standard sample [56] on a JASCO J-715 spectrophotometer (Jasco Corp., Tokyo, Japan). The quantitative content of polysaccharides was determined by the pharmacopeial method [33][34][35].

Chemical Profiling of LS-2 and LS-4 Extracts Using LC-DAD-QTOF-MS
Extracts LS-2 and LS-4 from S. deserta Schang. and S. sclarea L., obtained by ultrasonicassisted extraction, were concentrated to dryness using a rotary evaporator RV 10 digital V (IKA, Germany) under a temperature of 40-45 • C and further used for analysis. The liquid chromatographic system was an Agilent Series 1290, and the mass spectrometric analysis was performed with a QToF-MS/MS (Model #G6530A, Agilent Technologies, Santa Clara, CA, USA) equipped with an ESI source. All operations, acquisition and analysis of data were controlled by Agilent MassHunter Acquisition Software vA.01.00 (Agilent Technologies, Santa Clara, CA, USA) and operated under MassHunter Workstation software vB.02.00 (Agilent Technologies, Santa Clara, CA, USA). Each sample was analyzed in positive and negative modes to provide abundant information for structural identification [57]. Mass spectra were recorded across the range m/z = 50-1300 with accurate mass measurement of all mass peaks (Figure 4). MassHunter Workstation software, including Qualitative Analysis (vB.07.00), was used for processing both raw MS and MS-MS data, including molecular feature extraction, background subtraction, data filtering, and molecular formula estimation. The raw data were processed using the Find by Molecular Feature (MF) algorithm called Molecular Feature Extractor (MFE) within MassHunter Qualitative Analysis software (Agilent Technologies, Santa Clara, CA, USA). All the solvents, acetonitrile, methanol, formic acid used are of HPLC-certified grade, obtained from ThermoFisher Scientific (Hampton, NH, USA). Water for the mobile phase was purified using a Milli-Q system (Millipore, Burlington, MA, USA). Extracted molecular features were processed to create a list of compounds.

Animals
Male C57BL/6 mice 10-12-weeks old were purchased from SPF-vivarium of Masgut Aikimbayev's National Scientific Center for Especially Dangerous Infections (Almaty, Kazakhstan). The animals were housed in a temperature-controlled environment (23 • C) with 60% relative humidity applying a 12 h light/dark cycle. The animals had ad libitum access to food and water. Experimental procedures involving animals were in full compliance with current international laws and policies [58] and were approved by the Local Ethics Committee for Animal Use in the National Center for Biotechnology (Kazakhstan).

Cell Line
Neonatal human dermal fibroblasts (HDFn) employed in this study were obtained from Gibco (ThermoFisher Scientific, Carlsbad, CA, USA). HDFn were cultured in complete nutrient medium Dulbecco's Modified Eagle Medium (DMEM) containing 4.5 g/L glucose, 10% fetal bovine serum (FBS), 1% penicillin-streptomycin at 37 • C and 5% CO 2 . The adherent cells were passed with TrypLE ® Express (ThermoFisher Scientific, Paisley, UK) with an interval of 5-6 days. The medium in the cell culture was changed every two days. The cells were counted using a BioRad TC-20 automated cell counter (BioRad Laboratories, Hercules, CA, USA).

Isolation of Peritoneal Macrophages
Murine peritoneal macrophages were isolated according to a standard protocol described previously by Zhang et al. [59]. The mice were sacrificed by cervical dislocation and peritoneal macrophages were isolated by lavages of the peritoneal cavity with cold basal culture medium DMEM (Gibco, USA). The resident macrophages were collected by aspiration of the lavage fluid into a 10-mL syringe and harvested by centrifugation for 10 min at 400× g and 4 • C. The cells were resuspended in cold complete culture medium DMEM supplemented with 10% FBS (Gibco, USA) and 1% penicillin-streptomycin (Gibco, USA). The viability and number of peritoneal cells was determined by trypan-blue dye exclusion using a BioRad TC-20 automated cell counter (BioRad, Rocklin, CA, USA). In order to separate macrophages from other peritoneal cells, 2 × 10 6 nucleated cells were added to 6-well tissue culture plates and allowed to adhere to the substrate by culturing them for 1 h at 37 • C in CO 2 -incubator. Nonadherent cells were removed by gently washing three times with warm DMEM. After separation on cell adhesion, peritoneal macrophages were greater than 90%.

Isolation of Spleen Lymphocytes
Murine lymphocytes were isolated from the spleen according to a standard protocol described previously by Lim et al. [60]. For isolation of the spleen lymphocytes, animals were sacrificed by cervical dislocation. Spleens were removed aseptically and ground in glass homogenizers in cold RPMI-1640 medium (Sigma, USA) containing 2% FBS, penicillin (100 IU/mL)/streptomycin (100 µg/mL) and 55 µM 2-mercaptoethanol. Red blood cells (RBC) were lysed by treatment with RBC lysis buffer; cells were washed twice with phosphate buffered saline (PBS). Then, the cells were resuspended in RPMI-1640 medium supplemented with 5% FBS and penicillin (100 IU/mL)/streptomycin (100 µg/mL) to a final concentration of 7 × 10 6 cell/mL. Splenic B and T lymphocyte populations were fractionated by panning [61]. Plastic cell culture flasks were coated with the affinity-purified rabbit immunoglobulin (Ig) (Sigma, USA) with specificity for mouse Ig at a concentration of 200 µg/mL in 7 mL of PBS. After incubation overnight at 4 • C, the flasks were washed thrice with PBS and left for 45 min with RPMI 1640 medium. Spleen cells were then placed into the flasks in 7 to 8 mL aliquots. Flasks were incubated at 4 • C for 60 min with gentle stirring to redistribute cells every 30 min. Nonadherent cells were carefully poured off, washed once and resuspended into RPMI-1640 medium supplemented 10% FBS, penicillin (100 IU/mL)/streptomycin (100 µg/mL) and 55 µM 2-mercaptoethanol.

Cytotoxicity Test
HDFn were plated into the wells of a 96-well plate (BD Biosciences, Franklin Lakes, NJ, USA) at 1 × 10 4 cells/well. The cells were incubated in a high-glucose DMEM medium containing 10% FBS overnight at 37 • C and 5% CO 2 . Then, the culture medium was carefully replaced with fresh medium in a volume of 50 µL, and 50 µL of the plant extracts (LS-1, LS-2, LS-3, LS-4) were added. DMEM medium containing 10% FBS served as a control. The cells were incubated for 48 h at 37 • C and 5% CO 2 . After 48 h of incubation, the culture medium in each well of the 96 well plate was replaced with 90 µL of fresh DMEM medium without phenol red (Gibco, USA). Next, 10 µL of 3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide (MTT) solution (5 mg/mL) was added to each well. The cells were incubated for 4 h at 5% CO 2 and 37 • C. After incubation, the medium was carefully removed with an aspirator and formazan was dissolved by adding 100 µL of dimethyl sulfoxide (DMSO) per well. Optical density was recorded using a BioRad 680 plate spectrophotometer (BioRad Laboratories, Marnes-la-Coquette, France) at a wavelength of 580 nm. MTT test data were obtained by comparing the optical density (OD) in the experimental groups. The optical density is proportional to the number of living cells in the well. The change in OD was judged on the cytotoxic activity of the tested samples. The value of cell viability was calculated by the formula: A test/A control × 100, where A test is the optical density of the test wells; A control-the optical density of the control wells; 100-100% value.

Cytokine Detection in Cell Supernatants
The measurement of cytokine levels (TNF-α and IL-1β) in cell supernatants was carried out using enzyme-linked immunosorbent assay (ELISA). Peritoneal macrophages were incubated in the presence or absence of 1 µg/mL of bacterial lipopolysaccharides (LPS) (Merck, Darmstadt, Germany), and spleen lymphocytes in the presence or absence of 2.5 µg/mL with concanavalin A (ConA) (Sigma, USA). The cells were additionally stimulated with tested plant extracts (LS-1, LS-2, LS-3, LS-4) at a final concentration 50 mg/mL. ELISA was performed using the following kits: Mouse TNF alpha ELISA Kit and Mouse IL-1 beta ELISA Kit (Abcam, Cambridge, UK). In the wells of a 96-well MaxiSorb plate (Nunc, Rochester, NY, USA), 50 µL volumes of the tested samples were added to the wells of the plate. Staining was performed using antibodies labeled with streptavidin conjugated with horseradish peroxidase at a concentration of 1:250. Detection was performed using a TMB substrate Reagent Set (BD Biosciences, USA). The ELISA results were assessed visually and quantitatively using a BioRad 680 plate spectrophotometer (BioRad, France) at a wavelength of 450 nm. The data were processed using Zemfira 4.0 software (BioRad, Hercules, CA, USA).

Phagocytosis Assay
To determine the phagocytic activity, peritoneal macrophages were seeded into the wells of the 24-well culture plate at 5 × 10 5 cells/well. After 1 h incubation at 37 • C, the wells were washed two times with PBS. Then, plant extracts (LS-1, LS-2, LS-3, LS-4) diluted in complete culture medium DMEM were added to the wells containing monolayer of macrophages and incubated for 24 h at 37 • C and 5% CO 2 . The culture plate was then washed 3 times with PBS and diluted overnight bacterial culture of Escherichia coli (1:10) was added and incubated for 30 min with macrophages. After incubation, the cells were washed 3 times with PBS and fixed with 4% paraformaldehyde solution for 20 min. To stain gram-negative bacteria, 10 µL SYTO9 (ThermoFisher Scientific, Waltham, MA, USA) was added to each well and incubated for 10 min at room temperature. The phagocytic activity of macrophages was analyzed using an inverted fluorescence microscope Axio Observer A1 (Carl Zeiss Microscopy GmbH, Jena, Germany) and Zen 2011 software (Carl Zeiss Microscopy GmbH, Jena, Germany). The obtained images were processed using the ImageJ 1.52v software (NIH, Bethesda, MD, USA).

Statistical Analysis
All data are presented as mean ± standard deviation. The statistical significance was calculated using one-way analysis of variance ANOVA test. p < 0.05 was considered statistically significant. Statistical analysis was conducted with software Statistica 6.0 (StatSoft, Tulsa, OK, USA).

Conclusions
In the study the choice of the optimal technology for isolation of sage extracts included a number of parameters (extragent, ratio of extragent to raw material, time of extraction) provided for its most complete depletion in order to maximize the efficiency of obtaining the desired complex of valuable constituents, which demonstrated that under a significant extraction time reduction, ultrasound-assisted extraction allows obtaining a comparable amount of extracts and does not significantly impact the representation of major groups of BAC. Our study has demonstrated that the extracts of S. deserta Schang. (LS-2) and S. sclarea L. (LS-3) contain bioactive compounds, in particular polysaccharides that may stimulate or suppress the functional activity of peritoneal macrophages and T lymphocytes depending on their status. These results suggest that S. deserta Schang. and S. sclarea L. may be used as natural immunomodulators for the prevention and treatment of inflammatory diseases.

Conflicts of Interest:
The authors declare no conflict of interest.