Receptor-like Kinases (LRR-RLKs) in Response of Plants to Biotic and Abiotic Stresses

Plants live under different biotic and abiotic stress conditions, and, to cope with the adversity and severity, plants have well-developed resistance mechanisms. The mechanism starts with perception of the stimuli followed by molecular, biochemical, and physiological adaptive measures. The family of LRR-RLKs (leucine-rich repeat receptor-like kinases) is one such group that perceives biotic and abiotic stimuli and also plays important roles in different biological processes of development. This has been mostly studied in the model plant, Arabidopsis thaliana, and to some extent in other plants, such as Solanum lycopersicum, Nicotiana benthamiana, Brassica napus, Oryza sativa, Triticum aestivum, Hordeum vulgare, Brachypodium distachyon, Medicago truncatula, Gossypium barbadense, Phaseolus vulgaris, Solanum tuberosum, and Malus robusta. Most LRR-RLKs tend to form different combinations of LRR-RLKs-complexes (dimer, trimer, and tetramers), and some of them were observed as important receptors in immune responses, cell death, and plant development processes. However, less is known about the function(s) of LRR-RLKs in response to abiotic and biotic stresses. Here, we give recent updates about LRR-RLK receptors, specifically focusing on their involvement in biotic and abiotic stresses in the model plant, A. thaliana. Furthermore, the recent studies on LRR-RLKs that are homologous in other plants is also reviewed in relation to their role in triggering stress response processes against biotic and abiotic stimuli and/or in exploring their additional function(s). Furthermore, we present the interactions and combinations among LRR-RLK receptors that have been confirmed through experiments. Moreover, based on GENEINVESTIGATOR microarray database analysis, we predict some potential LRR-RLK genes involved in certain biotic and abiotic stresses whose function and mechanism may be explored.


Introduction
Biotic and abiotic stresses have detrimental effects on growth and development in plants that are at risk of biotic and abiotic stresses. To counteract these adversities, plants have developed diverse stimuli and activation strategies. The receptors in plants are one of the primary components of plant-environment interaction that transduces the information and makes the plant aware of its surroundings. In plants, a large number of different types of receptor-like kinases (RLKs) have evolved, and they are classified based on their kinase domain and extracellular domain sequences [1][2][3]. Among the RLKs, the biggest group are the Leucine-rich-repeats-RLKs (LRR-RLKs), which have an extracellular domain LRR motif that facilitates the binding of ligands (proteins, signaling peptides, hormones, etc.). In addition to their regulatory role in plant development, shoot and root apical meristem, xylem differentiation, and BL (Brassinolide) pathways, they also play a role in the immune system and activation of cell death [4][5][6][7][8][9]. A small number of LRR-RLKs have been shown the possibility of additional functions and their cross-link in triggering different signaling processes. Furthermore, we have presented the information on stress-related LRR-RLKs as dimer, trimer, and tetramer complexes, which were experimentally achieved in A. thaliana. Furthermore, an ATTED databases (https://atted.jp/hclust/, accessed on 2 February 2022) analysis for protein interactions pointed towards additional possible interactions among the LRR-RLKs, however, these need to be validated experimentally [33].

Abundance of LRR-RLKs Genes in the Plant Genome
The abundant availability of genome and RNA sequences allowed us to identify the potential LRR-RLK in different plants, such as annual and perennial plants, crops, trees, herbs, etc. (Table 1). In primitive plants such as Sedum alfredii, Selaginella moellendorffii, Amborella trichopoda, and Physcomitrella patens, there are about 60-134 genes. In higher plants, the identified LRR-RLK gene numbers vary from 200 to 600 (LRR-RLK genes) (See Table 1). It was shown that algae do not contain any LRR-RLK genes similar to plants and it was suggested that the structural combination of LRRs and kinase domains (KD) to form new genes may have occurred after the divergence of land plants from green algae [34]. An in silico analysis revealed that the expansion in LRR-RLKs is a result of tandem and segmental duplication events (Table 1). Additionally, the exon/intron compositions and motif arrangements were considerably conserved among members in the same groups or subgroups in plants (Table 1). Table 1. Number of LRR-RLKs present in different plants. Whole genome sequence data of the enlisted plant species was analyzed for the identification of LRR-RLKs. The identified LRR-RLK genes (numbers) with their expansion characteristic (presence of tandem duplication, motif conservation, and duplication) were based on different studies. Genome size for each species was given. Mb indicates mega base pairs. "-" indicates no data available.  The research on LRR-RLKs were mainly conducted in A. thaliana due to the availability of a large dataset. The increase in DNA and RNA sequencing in different crops and A. thaliana allowed for the identification of the numbers of stress-related LRR-RLKs (Table S1) and their sequence in other plants such as O. sativa, G. max, M. truncatula, Populus, V. vinifera, S. lycopersicum, B. napus, and Z. mays (Table S2). Note that some of the stress-related LRR-RLKs in A. thaliana have bigger numbers of orthologs in some plants, such as G. max, M. truncatula, O. sativa, and Z. mays, as compared to A. thaliana (Table S2). Only a few orthologs of stress-related LRR-RLK genes were investigated under biotic and abiotic stresses and their functions were elucidated in different plants by using different mutants of LRR-RLKs (Table S3).

Stress-Related LRR-RLKs in Plants
From a pool of 223 LRR-RLKs identified in A. thaliana ( Table 1), some were clustered as regulators of various growth and development processes (stem cell maintenance, anther development, determination of the fate of a cell and organ development, cell expansion, stem stomata development) [67][68][69][70][71], while others were shown or suggested to be important in biotic and abiotic stress responses ( Figure 1). One of the extensively studied genes among LRR-RLKs is BAK1/SERK3, which regulates multiple processes such as Brassinosteroid (BR) signaling, growth and development, and stomatal patterning, and also activates the expression of antimicrobial proteins [20,72]. Recently, the role of BAK1 in guard cell ABA signaling was demonstrated and bak1 mutants showed more loss of water as compared to wild-type. The ABA increased the formation of a complex between BAK1 and OPEN STOMATA1 (OST1) near the plasma membrane [73]. In addition, bak1-5 mutants revealed that the post-invasive resistance of A. thaliana to Alternaria brassicicola is independent of pathogen-triggered indole-3-carboxylic acid and its derivatives (ICAs) and camalexin biosynthesis [74]. Notably, the function of BAK1/SERK3 in the immune response was shown in tobacco and tomato plants against the late blight pathogen (Phytophthora infestans) and bacterial and nematode infection [75,76]. Other SERKs, such as SERK1 and SERK2, were also important genes for conferring resistance against bacterial leaf blight and fungal infection, respectively [20,77,78]. In rice, OsSERK2 was shown as a positive regulator of immunity, interacting with the rice immune receptor kinases (XA21 and XA3) [78]. It was suggested that SERK2 could be a target for sRNAs of Sclerotinia sclerotiorum and the resulting action may contribute to the silencing of immune components in plants [79]. Recently, using the serk2 mutant lines, it was observed that SERK2 is a component of BR signaling and it regulates BR signaling and salt resistance in rice [80]. Previously, SERK4 together with SERK3 were shown to trigger a series of defense responses [20], but in recent studies using knock-out and overexpressing lines of SERK4, it was observed that SERK4 negatively regulates the leaves senescence process [81].
Another well-studied LRR-RLK gene is BRI1, which interacts with SERK3 by mediating BR signaling through the BRI1/BAK1 complex [72], and it regulates stem elongation, vascular differentiation, seed size, fertility, flowering time, and senescence [8,17,82]. Moreover, the mutant of BRI1 shows pleiotropic effects on disease resistance along with plant development regulation [83], and it displays ABA-hypersensitive primary root growth [84,85]. In B. napus, S. lycopersicum, and B. distachyon, the role of BRI1 in BR signaling was confirmed [86][87][88]. Furthermore, BRI1 was involved in BR signaling through MAPK and Ca 2+ -dependent protein kinases in rice [89]. Additionally, it was suggested that SlBRI1 is related with systemin-mediated systemic defense response [90], however it was not established conclusively [87]. In tobacco, NbBRI1 was involved in BR-mediated systemic defense signaling by regulating H 2 O 2 and NO production [91]. Recently, BRI1 manipulation in different cereals resulted in drought tolerance [86], and disease resistance [83]. Additionally, the overexpression of wheat TaBRI1 in A. thaliana revealed early flowering and enhancement of seed production [92], while overexpression of SlBRI1 promoted fruit ripening and ethylene production, and increased the levels of carotenoids, ascorbic acid, soluble solids, and soluble sugars during fruit ripening [93]. In potato (S. tuberosum), BRI1 was involved in the regulation of tuberization, thus suggesting other possible roles of BRI1 [94]. BIR1 is another LRR-RLK that forms a complex with SERK3/BAK1 or with other SERKs, and these complexes were shown to repress the effector-triggered immunity (ETI) in the absence of pathogen effectors [72]. The LRR-RLK SOBIR1 also interacts with SERKs, serving as a stabilizer of the protein complex and aids receptor complexes in triggering defense responses [18]. Notably, the homolog of SOBIR1 activated the immune response in tomato against fungal infection [18], but not in antiviral infection [95]. In tobacco plants, NbSOBIR1 was involved in the immunity of N. benthamiana through monitoring the production of ROS [96]. Furthermore, the manipulation of GbSOBIR1 in cotton (G. barbadense) plants resulted in resistance to Verticillium [97]. While EFR regulated the immune response of plants after perceiving bacterial flagellin and EF-Tu by forming complexes with SERKs [72], it was observed that A. thaliana efr mutants lacking EF-Tu perception are more susceptible to transformation by Agrobacterium tumefaciens [98]. This revealed the importance of the EF-Tu perception system for plant defense. The homologs of EFR in tobacco and in tomato were also involved in pathogen-associated molecular patterns (PAMP)-triggered immunity, and suggesting transgenic expression of EFR could be used as an engineering tool against broad-spectrum bacterial infections [99]. In rice, it was shown that receptors EFR and XA21 recruit similar immune signaling [100]. The overexpression of AtEFR in different crops shows resistance to bacterial infection and/or symptoms. In wheat and apple, the overexpression of AtEFR enhanced resistance against bacterial disease, fire, and blight [101], respectively. Similarly, in M. truncatula, it reduced the bacterium infection [102] and in potato it enhanced bacterial wilt resistance [103]. SERKs also form complexes with PHYTOSULFOKINE (PSK) RECEPTOR 1 (PSKR1), which is known to regulate root growth and hypocotyl elongation [20]. Moreover, PSKR1 was also identified as an important component of plant defense [104]. PSKR1 suppresses salicylate-dependent defense responses, where the pskr1 mutants exhibit early senescence, a salicylate (SA)-associated response, and are impaired in wound healing, a jasmonate (JA)-associated response [104]. Moreover, OsPSKR1 was involved in the immune response against bacterial leaf streak in rice through salicylic acid (SA) pathway signaling [105]. PEP1 RECEPTOR 1 (PEPR1) and PEPR2 were involved in immune responses through interaction with SERK3 through AtPep1-triggered ROS production and ethylene signaling [20]. Furthermore, PEPR1 recognizes AtPep3 and increases tolerance to salt stress as well as immune response [106]. Furthermore, it was suggested that AtPeps-PEPR signaling pathway is involved in stomatal closure through an OST1-independent mechanism under biotic stress [107]. PEPR1/2 ORTHOLOG RECEPTOR-LIKE KINASE1 from S. lycopersicum was shown to regulate responses to systemin, necrotrophic fungi, and insect herbivory [108]. Previously, RECEPTOR-LIKE PROTEIN KINASE1 (RPK1) was linked to the water stress response [2], wherein it regulates ABA/stress signaling by controlling ROS homeostasis. Additionally, RPK1 and BAK1 form complexes with OST1 to regulate ABA-induced stomatal closure [73,109].
The LEUCINE-RICH REPEAT PROTEIN 1 (LRR1) forms a complex with pathogenesisrelated protein10 (PR10), and it leads to cell-death-mediated defense signaling [110]. Additionally, it was shown in vivo and in vitro that during plant responses to drought stress, LRR1 and KINASE 7 (KIN7) are degraded by PLANT U-BOX PROTEIN 11 (PUB11), an E3 ubiquitin ligase [111]. This KIN7 phosphorylates and activates tonoplast-located channels during ABA-and CO 2 -mediated stomatal closure [112]. Recently, Leucine-rich receptor-like kinase homologs in cereals such as barley and wheat showed important components of defense responses against Fusarium by disbalancing salicylic acid signaling [113]. The silencing of LRR1 in rice in the XA21 genetic background (XA21-LRR1Ri, XA21 is 21 amino acid tyrosine-sulfated epitope derived from the bacterial protein) compromises resistance to bacterial leaf blight, indicating involvement in XA21-mediated immune response [114]. Other LRR-RLK, RECEPTOR-LIKE KINASE 902 (RLK902) previously showed importance in resistance to Hyaloperonospora arabidopsidis (downy mildew) in A. thaliana [115]. RLK902 associates with ENHANCED DISEASE RESISTANCE 4 (EDR4) and with BRASSINOSTEROID-SIGNALING KINASE1(BSK1), a key component of plant immunity [116]. Not much is known about LRR-RLK-like NSP-interacting kinases (NIKs), such NIK1, NIK2, and NIK3, and their interaction with geminivirus nuclear shuttle protein (NSP). The binding of NSP to NIK inhibits its kinase activity in vitro, and a phenotypic analysis of NIKs mutant lines suggests that NIKs are involved in the antiviral defense response [117]. It was shown that At-FEI2 (cell wall receptor-like kinase) plays a positive role in Arabidopsis and in tomato defense against Botrytis cinerea based on the study using knockout mutants of the Bc-siR37 [118].
The LRR-RLK genes have been shown to play important roles in response to abiotic stresses. In previous studies, ERECTA-LIKE1 (ERL1), a member of the gene family closely related to LRR-RLKs, was shown to synergistically regulate plant development and morphogenesis and functions in response to abiotic stresses, especially for heat response and drought [2,119]. Recently, it was observed that the ERECTA family was involved in sensing salt and osmotic stresses [120]. Furthemore, other LRR-RLK genes like PXY-Like 2 (PXL2) have been proven to be essential in vascular development through recognizing small signaling peptides, and they play a role in ABA signaling [121]. While the PHLOEM INTERCALATED WITH XYLEM-LIKE 1 (PXL1) is essential in cold and heat stress (through the ROS), the PXL1 regulates HISTIDINE-RICH DEHYDRIN1 (AtHIRD1) and LIGHT-HARVESTING PROTEIN COMPLEX I (AtLHCA1) by phosphorylation [122]. Another LRR-RLK gene, such as SCHENGEN 3 (SGN3), is related with developmental processes, particularly with microdomain organization and enhanced suberization in the endodermis. Additionally, it was shown that the sgn3 mutant was extremely sensitive to environmental conditions, such as different temperatures and nutrient deficiency [123]. Moreover, the role of BRI1-LIKE3 (BRL3) was reported in sensing glucose and flg22, where BRL3 together with REGULATOR OF G-PROTEIN SIGNALING 1 (AtRGS1) prevents excess ROS burst and control growth inhibition [124]. Additionally, the overexpression of BRL3 resulted in drought stress tolerance in A. thaliana through the accumulation of osmoprotectant metabolites, such as proline and sugars [125]. In rice, the OsBRL1 and OsBRL3 were shown to be partly involved in BR perception in the roots [126]. Another LRR-RLK, RLK7/LRR XI-23, was involved in the regulation of seed germination and oxidative stress [127]. Furthermore, the LRR-RLKs, RECEPTOR-LIKE PROTEIN KINASE 2 (RPK2) also known as TOAD2) together with CLAVATA3 (CLV3), regulate the development of anthers, embryo, and stem cell homeostasis in the shoot apical meristem [2]. Single mutants of RPK2 and CLAVATA1 (CLV1) and CLAVATA2 (CLV2) showed importance in nematode parasitism [128]. Furthermore, RPK2 interacts with other LRR-RLK, such as SENESCENCE-ASSOCIATED RECEPTOR-LIKE KINASE (SARK)-also known as CLAVATA3 INSENSITIVE RECEPTOR KINASE 3 (CIK3)-to maintain stem cell homeostasis [129] and anther development [33]. SARK was shown to be a positive regulator of senescence through hormone imbalances [130]. Interestingly, PpSARK in moss was shown as a positive regulator of senescence in salt stress responses and was also suggested to be a negative regulator of senescence [131]. In common bean's SARK, it did not coordinate senescence in nodules [132].
Thus, the mentioned LRR-RLK gene(s), which were tested in different biotic or abiotic stress/senescence processes, shared similar signaling pathways, such as reactive oxygen species (ROS) production, Ca 2+ influx, activation of MAPK, regulation of defense genes, regulation of stomatal patterning, hormonal regulation, and regulation of senescencerelated gene(s), to trigger the response of plants (See the scheme in Figure 1). Remarkably, some LRR-RLK genes show crosstalk in triggering different programs ( Figure 1) and their numbers increased compared to the most recent review articles, which mentioned LRR-RLK genes in biotic and abiotic stresses [17,20]. Therefore, we have additionally collected all of the known single mutants of LRR-RLK genes and enlisted their phenotypic changes under experimentally tested biotic and/or abiotic stresses in Table S1, where their crosstalk can be easily observed. The information was gathered from the available microarray and RNA sequencing data (online available GENEINVESTIGATOR), which is also useful in predicting the potential role of genes for a particular stress stimulus [133]. Several LRR-RLK genes were identified in biotic or abiotic stresses (Table S1). The microarray data analysis of these LRR-RLK genes showed the alterations in expression under both types of stress stimuli i.e., biotic and abiotic ( Figure S1). For example, FEI2, NIK3, RLK902, RPK2, EFR, SOBIR1, and NIK1, which were tested only in biotic stresses, showed a change in the expression under abiotic stress as well ( Figure S2). Similarly, the reverse was observed for genes involved in abiotic stresses, such as RPK1, BRL3, ERL1, PXL1, PXL2, SARK, RLK7/LRR XI-23, SGN3, KIN7, and PEPR1, as it showed a change in expression for biotic stress too ( Figure S3). The detailed characterization of these LRR-RLK genes under both stresses is likely to provide additional information on their mechanistic role and may give insight into the crosstalk among the LRR-RLKs.
The formation of heterodimers with other LRR-RLKs, so-called co-receptors that usually have short extracellular domains, is important for holding the ligand and stabilizing it for the enhancement of the transduction of the intracellular signal [22]. The role of heterodimers was mainly as a defense response, development process, BR signaling, or cell death ( Figures S4-S7). For example, BRI1 generated complexes with SERKs (BAK1/SERK3, SERK1, and SERK4) and TTL3-only for BR pathway activation [16,140,141], BIR1 can interact with BAK1, SERK1, SERK2, and SERK4 to inhibit plant cell death [14,30], and EFR with SERK1, BAK1, and SERK4/BKK1 can activate the immune response [16,98] and EFR with GLYCINE-RICH PROTEIN7 (GRP7) activates the PAMP-triggered immune (PTI) response against Pseudomonas syringae [142]. SOBIR forms a complex with BAK1 for immune responses against P.infestans and S. sclerotiorum [14,30], and the heterotrimer with AtRLP23 is formed in the absence of BIR1 to activate cell death [14]. Additionally, PSKR1 may interact with SERK1/2 and BAK1 [143]. Furthemore, CLV1 forms heterodimers with BAM1 (BARELY ANY MERISTEM1), SARK, and CRN (CORYNE), RPK2 [32,138,144,145], which are important for apical and young floral meristem development. Conversely, other LRR-RLKs that form variant complexes were shown to perform different roles. For example, BAK1 interacts with FLS2, SOBIR1, NIK1, BKK1, BIR2, PEPR1, PSKR1, EFR, BIK1, RLP23, ERL2, ERL1, ER, and TMM to activate immune responses [14,16,[28][29][30][31]; with BIR1 to inhibit plant cell death; with RPK1, BIR1, BON1 (BONZAI1), SOBIR1, and HSL2 to activate cell death [30,109,146]; with BRI to activate BR pathway [8,16]; and with ER or ERL1 to regulate stomatal patterning [147] (Figure S4). Other multifunctional LRR-RLK genes are SERK1 and SERK2, which interact with EXCESS MICROSPOROCYTES 1 (EMS1) for anther development [135]; with PXY to regulate procambial cell proliferation [23,148]; with ER or ERL1 to regulate stomatal patterning; and with FLS2 and EFR, BRI1 and BAK1 for other functions ( Figure S5). Some multifunctional LRR-RLKs, such as BAK1, SERK 1, SERK2, SERK4, SARK, NIK1, and NIK3, were classified as co-receptors based on the length of the extracellular domain (ECD) [22], which are able interact with other LRR-RLKs, and they are grouped as the LRR  Table 2 and Table S4), which are classified as ligand-perceiving receptors with long ECD and are grouped as LRR (I), LRR (X), LRR (XI), and (LRR XII) [22]. Mainly, these co-receptors potentially bind with ligandperceiving receptor groups for the activation and stabilization of complexes that sense signals [8,13,16,22,28]. They also enhance signaling through sequential reciprocal receptor transphosphorylation [149]. Different combinations of interactions among co-receptors with different ligand-binding LRR-RLKs allows for the substitution of each other. This creates difficulties in using a single mutant line for LRR-RLK gene studies. For example, SERK1, SERK2, and BAK1 share the same players, like EFR and FLS2, although BAK1 is preferred by FLS2 among other SERKs, whereas EFR does not show preference to BAK1 [16]. Yet, SERK1 and SERK2 could easily substitute each other for activation of a similar plant response [16]. This regulation of stability of complexes is less studied, with only a few reported cases. For example, BAK1 interacts with BIR1 or BIR2 to prevent heterodimerization of the BAK1-FLS2 complex, and thereby inhibits an immune system response [14,30]. Furthermore, BRI1 and SERK3 do not ubiquitously interact, they only show interaction in the endosomes and in restricted areas on the plasma membrane. In these sites, BAK1 is shown as a redistributor of BRI1 receptors [15]. Thus, most LRR-RLKs form complexes, and some of the LRR-RLK in the complexes play key roles in the complex, such as BAK1 [16,28], and some components in the complexes are able to substitute for each other.
Additionally, the ATTED database showed different interactions among the proteins based on various experiments and data ( Table 2). The analysis of these interactions confirmed the known interactions of BAK1, SERK 1, SERK 2, SERK 4, NIK1, NIK3, and SARK ( Figures S4-S7), but also the additional potential heterodimerizations for these coreceptors ( Table 2). The less investigated co-receptors, like NIK1 and SARK, according to this database, have a potential for binding with BAK1. Importantly, the CIKs, such as NIK1, NIK3, and SARK, were shown in the stress response and in natural senescence [4,22], but their co-receptors' (heterodimers or homodimer) involvement in senescence or the stress response was not shown ( Table 2). It will be exciting in the future to reveal the additional functions of these CIKs and to show the new interactions of the collected LRR-RLKs mentioned in Table S4. The interactions among the LRR-RLKs in other plants are less studied, and only a few were confirmed in tomato and tobacco [75,150], rice [77], M. truncatula [151], and wheat [92] (Table S4), and that was mainly done by using the yeast two-hybrid assays, co-immunoprecipitation, and BiFC methods. Altogether, the study of new interactions of LRR-RLKs in A. thaliana and other plants and the generation of new double or triple mutants could help in functional and interactional analyses of LRR-RLKs. Table 2. Experimentally proven and potential interactions of stress-related LRR-RLKs with LRR-RLK co-receptors from LRR II. Data taken from different well-studied research manuscripts and from ATTED database. The formation of heterodimers between the LRR-RLKs were labeled as "heterodimer" inside of box cross between two LRR-RLKs one from column and second from row. Below the heterodimer formation were shown the method/s of identifications heterodimer formation such as CoIP-coimmunoprecipitation; BiFC-bimolecular fluorescence complementation, gel filtration, pull-down, in vivo, genetically (by mutants analysis), kinase assay, co-sedimentation in solution, solid-phase assay. NA indicates not available data reported the formation of heterodimer/s. Grey colored box indicates that information taken from ATTED database about interaction was confirmed experimentally (published data), and white zone was not confirmed experimentally.

Conclusions
Plants must evolve to adapt and tolerate harsh environments. The perception of biotic and abiotic stimuli is crucial for the survival of plants. Among the large numbers of receptors, the LRR-RLK are not only involved in different development biological processes but also in stress response processes. The rapid development of genome and RNA sequence analyses have allowed for the identification of the many LRR-RLKs genes in different monocot and dicot plants. As compared to A. thaliana, not much is known about the functions of LRR-RLKs in other plants such as tomato, tobacco, wheat, rice, H.vulgare, B. distachyon, etc. Additionally, stress-related LRR-RLKs in A. thaliana are mainly shown to play a role in biotic stresses, and to some extent in abiotic stresses. For the functional study of LRR-RLKs, a single mutant is of little use to capture all their functions, and, as LRR-RLKs can be substituted by other members, they do not have non-specific ligand binding and they have the ability to make different protein complexes (di-, tri-, or tetramer). The double, triple, and quadruple mutant generation for LRR-RLKs in A. thaliana and/or crop plants is a powerful tool for identifying the function role of LRR-RLKs.
Supplementary Materials: The following are available online at https://www.mdpi.com/article/ 10.3390/plants11192660/s1, Figure S1: Microarray analysis of the biotic and abiotic stress related LRR-RLK genes transcripts in response to biotic and abiotic stresses in WT (Col ecotype). Figure S2. Microarray analysis of the biotic stress related LRR-RLK genes transcripts in response to biotic and abiotic stresses in WT (Col ecotype). Figure S3. Microarray analysis of the abiotic stress related LRR-RLK genes transcripts in response to biotic and abiotic stresses in WT (Col ecotype). Figure S4. Protein interactions of BAK1/SERK3 with other LRR-RLK with experimental proved functional role. Figure S5. Protein interactions of SERK1 and SERK4 with other LRR-RLK with experimental proved functional role. Figure S6. Protein interactions of SERK2 with other LRR-RLK with experimental proved functional role. Figure S7. Protein interactions of CIK's (NIK1, NIK3 and SARK) with other LRR-RLK with experimental proved functional role. Table S1. Phenotypes of single mutants of LRR-RLK genes in A. thaliana tested under different biotic and abiotic stresses. Table S2. Orthologous of stress related LRR-RLKs genes in A. thaliana, O. sativa, G. max, M. truncatula, Populus, V. vinifera, S. lycopersicum, B. napus, Z. mays. Table S3. Genetic tools in different crop plants in investigation of stress-related LRR-RLKs. Table S4. Potential interactions of stress-related LRR-RLKs (from LRR II family) with other LRR-RLKs. References  are cited in the supplementary materials.