Discovery and Domestication of New Fruit Trees in the 21st Century

The exploration and use of wild plant resources goes back to our rooted history of human civilization over about 20,000 years ago, before Ancient Mesopotamia in the Valley of the Tigris and Euphrates where barley, lentil and wheat were first domesticated [...].


Fruit Domestication
The domestication of fruit crops has always been a long-term endeavor with terrible uncertainty and tremendous difficulty. In the 20th century, only four major fruit crops have been successfully domesticated from the wild, i.e., blueberry (Vaccinium L.), kiwifruit (Actinidia L.), avocado (Persea Mill.) and macadamia nuts (Macadamia F. Muell.). However, plant domestication is part of our civilization and has been a sustained and endless process throughout human history. In the beginning of the 21st century, the domestication of fruit crops has been arisen in many efforts of a few wild plants, such as Akebia trifoliata subsp. australis (Diels) T. Shimizu, Lonicera caerulea L., Kadsura coccinea (Lem.) A. C. Smith) etc. in China; Asimina triloba (L.) Dunal, etc. in North America; Aristotelia chilensis (Molina) Stuntz, etc. in South America [3].
In the history of fruit crop domestication [4], whether it is an apple, plum and grape etc. domesticated thousand years ago, or kiwifruit and blueberry etc. domesticated in the last century, a sustained purpose and clear domestication strategy have always been key factors for any successful domesticated fruit plants. Even nowadays in the 21st century in the domestication of new crop plants from the wild, the key towards success of the domestication of a new crop plant should be a long-term vision and well-formulated strategy of domestication, including clear goals of genetic improvement, an appropriate breeding approach and new breeding technologies employed. For example, a pioneer domestication program of Akebia, native to China in the wild, a well formulated strategy was drafted and put forward since the beginning of this century [5,6], with a set of six well-projected goals for domesticating improvements, including the traits of fruit such as, crackingness, fruit size, fruit peel thickness, seed numbers, fruit edible ratio and fruit storage [7]. This domestication program also integrates traditional breeding with current new technology, including selecting superior genotypes from wild populations, intraspecific and interspecific hybridization, recurrent and pedigree selection, molecular marker assisted selections and ploidy manipulation to improve the breeding efficiency.
Domestication of a new fruit plant may not be as easy as many plant breeders or pomologists thought. Apparently, apart from a well-projected goal of a set of traits to be genetically improved, germplasm resources in the starting point plays a crucial role for the success of domesticating a new fruit plant [8]. Among the hundreds of wild fruit plants with domesticating potential, only a few have been evaluated and used for serious domestication processes, exemplified by a general lack of understanding and efforts in germplasm evaluation at the starting stage of domestication. In fruit domestication, the human pursuit for new tasting fruits are endless. Of course, nowadays, people are increasingly pursuing fruits with a good taste, nutrition and health care. The introduction and domestication of new fruits is more inclined towards the exploration and utilization of wild fruit tree resources with high nutritional and therapeutic value. New fruit discoveries from the wild have obvious advantages in nutrition, therapeutic value and novel taste. However, compared with commercial fruits, there are some characteristics such as poor palatability, small fruit and low yield must to be improved to meet the market acceptability. An appropriate breeding and selection goal is very important for the domestication of wild fruit trees. Firstly, a broad spectrum of wild germplasm resources is systematically investigated, collected and evaluated to lay a solid foundation for their continuous variety improvement. Second, market feedback is one of the required endeavors for fruit tree breeders. The timely understanding of the market recognition of varieties can avoid the inertia of "self-entertainment" of breeders in the breeding orchards. From the perspective of domestication and improvement methods of wild fruit plants, conventional breeding methods are still the main technique in the initial domestication stage of new fruits, especially natural variation selection, intra and interspecific hybridization, systematic breeding and so on. With the advancement of domestication process, modern biotechnology assisted breeding and genetic manipulation can be used to speed up the domestication and genetic improvement process [9].

Germplasm Collections
Germplasm collections primarily provide the raw material for programs of any plant breeding and genetic improvement. Hundreds of living germplasm collections of fruit tree crops across the world have played an incredible role in fruit tree breeding and enabled many newly improved cultivars available to the worldwide fruit industry. Germplasm repositories for fruit trees are uniquely constructed as clonal living collections preserved in orchards, vineyards and plantations or nurseries, etc. including diverse valuable resources such as current commercial cultivars, traditional cultivars, landraces, elite lines, and other breeding materials or wild relatives [10,11]. In addition to the preservation and maintenance of well-documented germplasm, fruit tree living collections are not only used by fruit breeders but other biologists who may have different interests and possibly different inquiries. While fruit breeders focus on commercial traits of immediate perceived value, other biologists may be more interested in investigating the properties and behaviors of the plant, domestication history, evolutionary phylogeny, especially at the genomic level.
One of the prioritized tasks in a germplasm repository of fruit tress is to characterize the genetic diversity and composition of accessions maintained in the repository to capture an as broad as possible genetic and morphological diversity, to facilitate the characterization of intra-and interspecific variation, to understand phylogenetic relationships among all resources including cultivars, varieties, subspecies and species, particularly those between wild relatives. The quantity and quality of data documentation are of crucial importance for germplasm repository management. Although data documentation changed over time with initial morphological evaluation, genomic data have been increasingly obtained from a wide range of fruit tree germplasm collections. Genotyping accessions have become routine protocol in fruit tree germplasm collections to verify pedigrees and track a trait of interest in breeding. Nevertheless, genetic information by using SSR, SNP and AmpSeq, etc. provides valuable a resource for breeding designs of fruit trees regardless of traditional breeding or molecular breeding programs, in particularly, parentage choice. The nature of fruit trees as mostly perennial plants also provides other biologists the ability to study and understand many aspects about basic plant biology, such as growth, development, reproduction, biotic and abiotic stress tolerance, as well as metabolites changes etc. due to germplasm repository of fruit trees maintained in the same location across multiple years, which offer important opportunities to study inter-annual variation under common-garden conditions. One approach, for example, is to study phenotypic plasticity in response to climatic changes and adaptive evolution of long-lived plants.
Ultimately, fruit tree living collections are of particular use for fruit tree breeding and genetic improvement for new cultivar development, for example, many new kiwifruit cultivars recently released to the worldwide kiwifruit industry rely heavily on the Chinese National Kiwifruit Germplasm Repository at the Wuhan Botanical Garden, Chinese Academy of Sciences. Recently, molecular approaches such as GWAS, QTLs and other marker-assisted selection have been increasingly used as tools for selecting potential parents from germplasm collections for use in many fruit tree breeding programs to accelerate the breeding process [12]. It is evident that fruit tree improvement could greatly be enhanced by standardized and statistically robust procedures for discovering quantitative trait loci (QTLs) in germplasm relevant to breeding programs and aided for validation of important breeding parents (IBPs) by estimating average allelic representation in wild relatives, which also demonstrated the importance of constructing a core germplasm set in fruit tree living collections [11].
Looking to the future, what new fruit crops could be domesticated in the new century? When economical and societal development are rapidly evolving, the elevation of living standards and awareness of health care have increasingly challenged fruit breeders for novel and high nutritional or functional fruits that are of benefit to human health. The new fruit types are becoming increasingly in high demand in the marketplace. No doubt fruit breeders will continue to seek new possibilities to domesticate new fruit crops. Apart from their ongoing improvement of current fruit crops, fruit germplasm curators put their efforts in the discovery of new potential fruit crop germplasm sources from collections or in the wild. Domesticating new plant crops is in fact never stalled, but an ever-continuous process in our mankind civilization and history. The incentive was originally driven by the endless pursuit for new taste of fresh fruits, but evolved to the preference of taste, nutrition and health care. Fundamentally, the domestication of new fruits relies on the discovery of the wild fruit plants with high nutritional and food therapeutic value.

Ex Situ Flora for the Sustainable Future
The planetary scale changes characterizing the Anthropocene have substantially transformed natural vegetation for millennia, most notably as a result of the Columbian exchange in the past 500 years [13,14], when increasing globalization and agricultural expansion has led to dramatic plant species extinction and an increasing homogenization of global biodiversity [15,16]. In this context, plant conservation in the Anthropocene is extremely challenging and despite strenuous efforts, the Aichi Biodiversity Targets have not been met [17]. Now, the emerging post-2020 global biodiversity framework (GBF) is under fire over the setting of hotly debated goals, while the deep philosophical connections between humanity and biodiversity inevitably need to be addressed. From the historical hunting and gathering for food and drugs from plants to today's active efforts to cultivate rare and threatened plants. Germplasm, botanists, gardeners and living plant germplasm curators acting as anthropogenic agents of dispersal have generated extraordinarily diverse ex situ floras in marked contrast to the less than a quarter of the unused wild and mostly fragmented flora remained in situ around the globe.
Currently, there are ca. 30% of the world's known plant species conserved in botanical gardens or other germplasm living collections around the world [18], highlighting the global socioeconomic importance of anthropogenic ex situ plants. As a key example, the ex situ Flora project in China, which was initiated in 2012 [19], was the first extensive inventory of ex situ Flora by any megadiverse country of the plants conserved in ex situ collections. The project has resulted in the 20 published volumes of the ex situ Flora of China and brought together a combined mega-database of the living plant inventory. Analyses of this database reveal that the ex situ Flora of China comprises of more than 28,000 species belonging to >3500 genera and >310 families, approximately equating to >7% of known

Conflicts of Interest:
The authors declare no conflict of interest.