Novel Neuroprotective Potential of Bunchosia armeniaca (Cav.) DC against Lipopolysaccharide Induced Alzheimer’s Disease in Mice

Bunchosia armeniaca (Cav.) DC (Malpighiaceae) is one of the well-known traditionally used remedies worldwide. This study aims to explore the leaves’ metabolome via Quadrupole-Time-of-Flight-Liquid-Chromatography-Mass Spectrometry and to investigate the neuroprotective effect of leaves using lipopolysaccharide (LPS) induced Alzheimer’s disease model. Mice were administered LPS (0.25 mg/kg/day; intraperitoneal) as well as methanolic extract (BME), dichloromethane (BDMF), and butanol (BBF) fractions (each 200 mg/kg/day; oral) for one week. BME and BBF improved behavioral activity on the Y maze test, decreased brain content of inflammatory markers such as nuclear factor kappa B and interleukin 1 beta, and prevented the elevation of cytochrome P450 2E1, and glial fibrillary acidic protein compared to the LPS-administered group. Histopathological examination of several brain parts confirmed the neuroprotective effect of the tested extracts. In addition, BBF exhibited higher activity in all tested in vitro antioxidant and acetylcholinesterase inhibition assays. Metabolic profiling offered tentative identification of 88 metabolites, including mainly flavonoids, phenolic acids, and coumarins. Several detected metabolites, such as quercetin, apigenin, baicalin, vitexin, and resveratrol, had previously known neuroprotective effects. The current study highlighted the possible novel potential of B. armeniaca in preventing memory impairment, possibly through its antioxidant effect and inhibition of acetylcholinesterase, inflammatory and oxidative stress mediators.


Introduction
The genus Bunchosia, family Malpighiaceae, includes approximately 75 species native to America and found mainly in Amazonia, Atlantic Forest, and Pantanal of Brazil [1,2]. Bunchosia armeniaca (Cav.) DC. is native to Colombia, Ecuador, Peru, and Bolivia in the Andean region. It is a rare plant in Brazil, although it is widely grown in other South American countries [3]. The genus name derives from one of the Arabic names, "buncho", for the coffee plant (Coffea arabica L.) because of the resemblance of the seeds. In contrast, the species name refers to the shape and color of the fruits, such as apricots (Prunus armeniaca L.) [4]. In 1789, it was given the name Malpighia armeniaca, and renamed Bunchosia armeniaca in 1824. This species' seeds, or pits, are poisonous, although the fruits are edible. The softened red fruits have a strawberry jam taste and a peanut butter consistency [5]. This plant has been

Metabolic Profiling Using Q-TOF LC/MS/MS
LC-MS approaches are anticipated to be of exceptional utility in plants. They have a reasonably rich biochemistry that comprises several semi-polar components, including crucial secondary metabolite classes, that may be efficiently separated and identified using LC-MS techniques [33]. Electrospray ionization (ESI), in both positive and negative ion modes, has swiftly become the method of choice for sugar sequence analysis, especially when combined with MS-MS techniques. The approach helps use minimal quantities of constituents (less than 1 mg) and facilitates rapid analysis of pure and mixed samples with no need for derivatization [34].
Metabolomic analysis of BME leaves using Q-TOF LC/MS/MS was adopted to unravel the phytoconstituents. Negative and positive modes were performed in the current study, leading to the tentative identification of 88 compounds from distinct phytochemical classes in B. armeniaca leaves ( Table 1). The identified compounds included 13 amino acids, 3 alkaloids, 6 phenolic acids, 36
Previous studies reported the presence of caffeine in Bunchosia glandulifera fruit, which is consistent with our findings [32,60]. However, to our knowledge, this is the first report for the detection of trigonelline and harmaline in Bunchosia, while trigonelline was previously isolated from the leaves of Niedenzuella multiglandulosa (Malpighiaceae) [61].

Polyphenols
As many as nine polyphenolic compounds were presented in B. armeniaca, including two stilbenoids (peaks 27 [62]. Astringin (peak 78) was detected by a precursor ion at m/z 405.1771, giving the characteristic fragment ion at m/z 243.0687 (−162 Da) [63]. Epicatechin (peak 87) demonstrated a deprotonated ion at m/z 289.0696 and a distinctive product ion at m/z 245.0605 due to loss of CO 2 or CH 2 -CHOH moieties (−44 Da) [56,64]. All polyphenolic compounds in this study were identified for the first time in the genus Bunchosia and the plant B. armeniaca.
The use of both positive and negative ionization LC-MS provides more comprehensive metabolome coverage than the use of a single mode. Several analytes could only be identified in the negative ion mode, whereas others could only be detected in the positive ion mode. Dual ionization modes were used to identify common differential metabolites such as taxifolin, acacetin, baicalin, isoorientin, astringin, robinin, vitexin, puerarin, tulipanin, spiraeoside, luteolin, narcissin, myricetin, juglalin, daphnetin, chlorogenic acid, acaciin, and hymecromone. The study of the fragmentation pathway of (M − H) − /(M + H) + ions in the negative and positive ion modes aided in the identification of these compounds. Most identified constituents were phenolics and flavonoids, which are known to be beneficial to human health. Various studies have documented flavonoids with a wide range of pharmacological activities, including antioxidant, anti-inflammatory, hepatoprotective, and antiangiogenic properties [65], as well as being antidiabetic [66], cardioprotective [67], neuroprotective, and anti-Alzheimer's disease [68], which are primarily due to their degree of hydroxylation, structural class, other substitutions, conjugations, and degree of polymerization, as well as metal chelation activity [69].

Antioxidant Activity
Antioxidants protect the body from illnesses such as AD, cancer, atherosclerosis, and obesity. The high antioxidant activity of the plant is a prior indication of its potential anti-Alzheimer properties [70]. Plants, vegetables, and fruits are currently the primary sources of antioxidant-active chemical constituents, where their phenolic contents are thought to be responsible for the strength of this antioxidant activity [71]. The antioxidant potential of a plant is defined as the degree to which its antioxidant compound prevents or inhibits the oxidation of biomolecules in the environment [72]. Antioxidant activity is a multi-step process involving multiple mechanisms regulated by various elements that cannot be fully described by a single method. In order to account for the varied mechanisms of antioxidant action, it is necessary to undertake multiple types of antioxidant capacity measurements [73]. Because many plants are complex, it is impossible to assess the antioxidant activity of B. armeniaca using one technique. In the current research, three complementary assays were performed (DPPH, ABTS, and FRAP) to evaluate the antioxidant effect of B. armeniaca leaves. Fractionation of B. armeniaca crude extract resulted in successive fractions with variable degrees of antioxidant activity.

DPPH Radical Scavenging Assay
Antioxidant components and radical scavengers are utilized as essential supplemental nutrition that may help protect human health from various diseases [74]. The DPPH radical scavenging test is a helpful approach for assessing the antioxidant properties of natural and synthetic substances. It is among the most potent, efficient, accurate, easy, and reproducible in vitro techniques for determination of this essential activity of single constituents and plant extracts [75]. Using the DPPH test, the radical scavenging activities of the extract and fractions of B. armeniaca leaves were evaluated and represented in (Table 2). When compared to BME (IC 50   According to previous research, the IC 50 value of B. armeniaca fruit's methanolic extract was 3.443 ± 0.29 mg/mL, while the gallic acid standard had an IC 50 value of 0.0036 mg/mL [5]. The difference between the current research value and the previous one mentioned above could be due to the plant components tested being different.

ABTS Cation Radical Decolorization Assay
The ABTS radical scavenging assay can be used for food and plant extracts, including lipophilic and hydrophilic substances. It depends on the suppressed absorbance of radical cation ABTS+, which has a particular color at 734 nm. As demonstrated in (Table 2), the results of the ABTS assay of the extracts and fractions showed high similarity with the results from the DPPH assay. BBF showed the most potent free ABTS radical-scavenging effect with IC 50 of 15.59 ± 0.37 µg/mL even compared to standard Trolox (IC 50 : 22.56 ± 0.78 µg/mL). However, BDMF showed relatively moderate results with IC 50 of 84.91 ± 2.46 µg/mL followed by BME with IC 50 of 121.27 ± 4.8 µg/mL.
The ABTS assay was used for the first time in this work to investigate the antioxidant properties of B. armeniaca. On the other hand, a previous study employed this method to assess Bunchosia glandulifera's antioxidant efficacy, yielding a value of 8928.57 µM of Trolox Equiv./100 g of fruit pulp [60].

Ferric Reducing Antioxidant Power (FRAP) Assay
Natural products' ability to reduce metals is regarded as a key marker of their antioxidant properties [76]. Spectroscopically, the quantity of ferric (Fe 3+ ) complex conversion to ferrous (Fe 2+ ) form is detected by the color change of the solution based on the compound's ability for reduction. The increased reduction capability of the studied sample is shown by the higher absorbance of the reaction mixture [73]. FRAP values of B. armeniaca extract and its fractions were recorded in (Table 2) and expressed as EC 50 (µg/mL). BBF had the highest reducing power (100.3 ± 4.26 µg/mL), which was nearly similar to the Trolox standard (96.97 ± 2.73 µg/mL), followed by BDMF (255.4 ± 15.37 µg/mL), while BME was the least efficient (384.7 ± 9.8 µg/mL) to quench iron.
The FRAP assay has never been used to study the antioxidant activity of B. armeniaca. However, Bunchosia glandulifera was previously proven to have an antioxidant effect, which revealed 19,285.21 FeSO 4 µM/100 g of fruit pulp [60].
Finally, all antioxidant assays (DPPH, ABTS, and FRAP) revealed similar trends, with BBF having the highest antioxidant capacities. Therefore, these findings confirmed that BBF is enriched with active polar ingredients responsible for the antioxidant properties. The presence of considerable amounts of TPC and TFC in B. armeniaca may contribute to its potent antioxidant action [77]. Metabolic profiling of B. armeniaca extract revealed an abundance of phenolic compounds, implying that the plant is a powerful antioxidant. The potent radical scavenging potency may be explained by the presence of apigenin, quercetin, luteolin, naringenin [78,79], chlorogenic acid, hyperoside, spiraeoside, quercetin, epicatechin, and procyanidin B2 [80].

Anticholinesterase Activity
AD causes neurodegeneration, which leads to cognitive problems and eventually death. Cholinesterase inhibitors are still used to treat symptoms and cognitive impairment in Alzheimer's patients [81]. The results of IC 50 values for acetylcholinesterase (AChE) inhibition of tested samples are represented in (Table 3). Both BBF and BME were potent inhibitors of AChE with IC 50 values of 16.4 ± 0.81 and 31.5 ± 1.6 µg/mL, respectively, whereas BDMF had no activity when compared to reference donepezil (IC 50 = 7.89 ± 1.3 µg/mL). The current in vitro investigation emphasizes, for the first time, the beneficial effect of B. armeniaca leaves on AD. It provides a potential indicator for conducting an in vivo study using diverse parameters.

Anti-Alzheimer In Vivo Study 2.4.1. Acute Toxicity
Male and female mice treated with a single dose of 5 g/kg BME showed no signs of toxicity such as mortalities, hair loss, diarrhea, patches of yellow coloration, or behavioral abnormalities. Therefore, the acute lethal toxicity test revealed the safe oral administration of B. armeniaca extract.

Effects on Behavioral Activity on Y-Maze
LPS has been linked to an increase in amyloid beta (Aβ) accumulation, exhibiting pathological abnormalities such as memory deficiency and neuroinflammation. AD is characterized by memory loss, which plays a crucial role in the disease's progression [82]. LPS caused memory impairment as it decreased Y-maze alteration by 58% as compared to normal control. While BME, BDMF, and BBF improved the behavioral activity on the Y-maze by 88%, 58%, and 68%, respectively, as compared with LPS-administered mice ( Figure 3). As a result, BME demonstrated a significant reduction in exploratory behavior followed by BBF, suggesting that they possibly act as learning and memory enhancers.
Numerous previous studies have bolstered the idea that components with a natural origin, primarily flavonoids, have a beneficial role in improving learning and memory functions [83][84][85][86][87]. Furthermore, quercetin has a potent effect on memory and cognitive functions [88,89]. Another previous study demonstrated that quercetin treatment significantly increased the percentage of spontaneous alteration behavior, implying that it enhanced the spatial working memory performance of the LPS-administered mice [90]. Consequently, the flavonoid content in B. armeniaca may be responsible for the observed memory and learning improvements. This is the first report that B. armeniaca reduces learning and memory deficiencies in mice. Meanwhile, previous findings showed that Heteropterys aphrodisiaca, Malpighiaceae, had a similar effect on elderly rats [91].
hanced the spatial working memory performance of the LPS-administered mice [9 sequently, the flavonoid content in B. armeniaca may be responsible for the o memory and learning improvements. This is the first report that B. armeniaca reduces learning and memory deficie mice. Meanwhile, previous findings showed that Heteropterys aphrodisiaca, Malpi had a similar effect on elderly rats [91]. In the central nervous system, glial cells maintain the neuron's normal struc defend against pathogens [92]. Inflammation induces glial cell activation and rel flammatory mediators, leading to AD [93]. LPS is a well-known brain macrop ducer and immune system activator [94]. It stimulates microglial cells, resultin production of reactive oxygen species (ROS), nitrogen oxide species (NOS), and a of proinflammatory cytokines such as IL-1β, IL-6, tumor necrosis factor (TNF Figure 3. Effects of BME, BDMF, and BBF on behavioral activity on Y-maze in LPS-induced Alzheimer in mice. Data are presented as the mean ± standard error (SE) of n = 8 per each group. Statistical analysis was done using one-way analysis of variance followed by Fisher's LSD test. a Statistically significant from normal control at p < 0.05. b Statistically significant from LPS control at p < 0.05. c Statistically significant from BME at p < 0.05. BME: B. armeniaca methanolic extract; BDMF: B. armeniaca dichloromethane fraction; BBF: B. armeniaca butanol fraction; LPS: lipopolysaccharide.

Effects on NF-κB and IL-1β
In the central nervous system, glial cells maintain the neuron's normal structure and defend against pathogens [92]. Inflammation induces glial cell activation and releases inflammatory mediators, leading to AD [93]. LPS is a well-known brain macrophage inducer and immune system activator [94]. It stimulates microglial cells, resulting in the production of reactive oxygen species (ROS), nitrogen oxide species (NOS), and a variety of proinflammatory cytokines such as IL-1β, IL-6, tumor necrosis factor (TNF)-α, and interferons (IFNs) [94][95][96]. IL-1β expression plays a significant role in neuroinflammation and is one of the most critical neuropathological variables in neurodegenerative disorders, such as AD. Reduced detrimental effects induced by IL-1β may pave the way for AD treatment [97]. Moreover, NF-κB modulation would provide an approach for AD management by suppressing neuroinflammation and amyloidogenesis [98].
LPS injection expressed inflammatory mediators in the brain such as NF-κB and IL-1β by 309% and 193%, respectively, as compared to normal control. Moreover, BME, BDMF, and BBF reduced brain contents of NF-κB by 72%, 65%, and 66% ( Figure 4A), and IL-1β by 62%, 42%, and 54% ( Figure 4B), respectively, as compared to LPS-administered mice. Interestingly, treatment with BME returned NF-κB and IL-1β in the brain to their normal levels, whereas BBF restored NF-κB level to normal and produced a significant reduction in IL-1β level.
LPS injection expressed inflammatory mediators in the brain such as NF-κB and IL-1β by 309% and 193%, respectively, as compared to normal control. Moreover, BME, BDMF, and BBF reduced brain contents of NF-κB by 72%, 65%, and 66% ( Figure 4A), and IL-1β by 62%, 42%, and 54% ( Figure 4B), respectively, as compared to LPS-administered mice. Interestingly, treatment with BME returned NF-κB and IL-1β in the brain to their normal levels, whereas BBF restored NF-κB level to normal and produced a significant reduction in IL-1β level. Data are presented as the mean ± standard error (SE) of n = 8 per each group. Statistical analysis was done using one-way analysis of variance followed by Fisher's LSD test. a Statistically significant from normal control at p < 0.05. b Statistically significant from LPS control at p < 0.05. c Statistically significant from BME at p < 0.05. d Statistically significant from BDMF at p < 0.05. BME: B. armeniaca methanolic extract; BDMF: B. armeniaca dichloromethane fraction; BBF: B. armeniaca butanol fraction; LPS: lipopolysaccharide; NF-κB: nuclear factor kappa B; IL-1β: interleukin 1 beta.
Quercetin reduced the inflammatory cytokines such as TNF-α and NF-κB [99]. Apigenin also significantly attenuated LPS-induced inflammatory response, TNF-α, IL-1β, and IL-6 production as well as suppressed LPS-induced NF-κB stimulation [100]. Moreover, it enhanced expression of brain-derived neurotrophic factor (BDNF) both alone and after an inflammatory stimulus with IL-1β, an action that could be linked to anti-inflammatory and neuroprotective properties [97]. Therefore, the presence of various flavonoids in BME, such as quercetin and apigenin, supports the therapeutic neuroprotective effect of B. armeniaca leaves.
The neuroprotective effect of B. armeniaca, by suppressing NF-κB and IL-1β, was reported here for the first time. Hiptage benghalensis, Malpighiaceae, showed anti-inflammatory effects through inhibition of NF-κB in LPS-stimulated mouse cell line macrophages [101]. Data are presented as the mean ± standard error (SE) of n = 8 per each group. Statistical analysis was done using one-way analysis of variance followed by Fisher's LSD test. a Statistically significant from normal control at p < 0.05. b Statistically significant from LPS control at p < 0.05. c Statistically significant from BME at p < 0.05. d Statistically significant from BDMF at p < 0.05. BME: B. armeniaca methanolic extract; BDMF: B. armeniaca dichloromethane fraction; BBF: B. armeniaca butanol fraction; LPS: lipopolysaccharide; NF-κB: nuclear factor kappa B; IL-1β: interleukin 1 beta.
Quercetin reduced the inflammatory cytokines such as TNF-α and NF-κB [99]. Apigenin also significantly attenuated LPS-induced inflammatory response, TNF-α, IL-1β, and IL-6 production as well as suppressed LPS-induced NF-κB stimulation [100]. Moreover, it enhanced expression of brain-derived neurotrophic factor (BDNF) both alone and after an inflammatory stimulus with IL-1β, an action that could be linked to anti-inflammatory and neuroprotective properties [97]. Therefore, the presence of various flavonoids in BME, such as quercetin and apigenin, supports the therapeutic neuroprotective effect of B. armeniaca leaves.
The neuroprotective effect of B. armeniaca, by suppressing NF-κB and IL-1β, was reported here for the first time. Hiptage benghalensis, Malpighiaceae, showed anti-inflammatory effects through inhibition of NF-κB in LPS-stimulated mouse cell line macrophages [101].

Effects on CYP2E1 and GFAP
In previous studies, the activation of glial cells produced ROS, inducing neuronal degeneration [102,103]. CYP2E1 is the most efficient P450 enzyme which initiates NADPHdependent lipid peroxidation in most biological membranes and generates ROS [104]. Our results revealed that LPS injection elevated oxidative stress as evidenced by the elevation of CYP2E1 brain content by 235% as compared to normal control. While BME, BDMF, and BBF decreased activated CYP2E1 by 69%, 44%, and 61%, respectively, as compared to LPS-administered mice ( Figure 5A). Consequently, BME treatment restored CYP2E1 to its normal level, whereas administration of BBF significantly reduced it. Previously, natural flavonoids such as quercetin rescued neuronal degeneration by decreasing activated cytochrome c [90]. GFAP with amyloid deposition, provoking AD [106]. In the current study, LPS activated astrocytes and elevated GFAP brain expression by 276% as compared to normal control. Whereas BME, BDMF, and BBF decreased GFAP by 60%, 34%, and 54%, respectively, as compared to LPS-administered mice ( Figure 5B). Accordingly, BME and BBF were found to be effective in reducing the immunological reactivity of GFAP.
No previous studies reported the effects of any member of the genus Bunchosia on CYP2E1 and GFAP. Figure 5. Effects of BME, BDMF, BBF on CYP2E1 (A) and GFAP (B) in LPS-induced Alzheimer in mice. Data are presented as the mean ± standard error (SE) of n = 8 per each group. Statistical analysis was done using one-way analysis of variance followed by Fisher's LSD test. a Statistically significant from normal control at p < 0.05. b Statistically significant from LPS control at p < 0.05. c Statistically significant from BME at p < 0.05. d Statistically significant from BDMF at p < 0.05. BME: B. armeniaca methanolic extract; BDMF: B. armeniaca dichloromethane fraction; BBF: B. armeniaca butanol fraction; LPS: lipopolysaccharide; CYP2E1: cytochrome P450 2E1; GFAP: glial fibrillary acidic protein.
A previous study found that baicalein had the beneficial effect of diminishing the immunological reactivity of GFAP found in neurons [107]. Resveratrol, a natural polyphenol, has also been identified to have a possible therapeutic effect on lowering GFAP levels, which may help to reduce the risk of neurodegenerative disorders, especially AD [108,109]. As a result, the above-mentioned metabolites in BME might explain the activity of B. armeniaca in ameliorating LPS-induced AD. Data are presented as the mean ± standard error (SE) of n = 8 per each group. Statistical analysis was done using one-way analysis of variance followed by Fisher's LSD test. a Statistically significant from normal control at p < 0.05. b Statistically significant from LPS control at p < 0.05. c Statistically significant from BME at p < 0.05. d Statistically significant from BDMF at p < 0.05. BME: B. armeniaca methanolic extract; BDMF: B. armeniaca dichloromethane fraction; BBF: B. armeniaca butanol fraction; LPS: lipopolysaccharide; CYP2E1: cytochrome P450 2E1; GFAP: glial fibrillary acidic protein.
Glial cells, including astrocytes and microglia, have been correlated with neurodegenerative disorders such as AD [105]. When astrocytes were activated, they expressed GFAP with amyloid deposition, provoking AD [106]. In the current study, LPS activated astrocytes and elevated GFAP brain expression by 276% as compared to normal control. Whereas BME, BDMF, and BBF decreased GFAP by 60%, 34%, and 54%, respectively, as compared to LPS-administered mice ( Figure 5B). Accordingly, BME and BBF were found to be effective in reducing the immunological reactivity of GFAP.
No previous studies reported the effects of any member of the genus Bunchosia on CYP2E1 and GFAP.
A previous study found that baicalein had the beneficial effect of diminishing the immunological reactivity of GFAP found in neurons [107]. Resveratrol, a natural polyphenol, has also been identified to have a possible therapeutic effect on lowering GFAP levels, which may help to reduce the risk of neurodegenerative disorders, especially AD [108,109]. As a result, the above-mentioned metabolites in BME might explain the activity of B. armeniaca in ameliorating LPS-induced AD.

Histopathological Examination
A histopathological examination of different brain tissues was conducted to provide a supplementary explanation of the brain alterations induced by LPS and the effects of BME, BDMF, and BBF administration. Microscopical examination of the cerebral cortex ( Figure 6) revealed no histopathological alteration in normal control sections ( Figure 6A). While in mice injected with LPS, cerebral cortex sections had nuclear pyknosis and neuronal degeneration with focal gliosis ( Figure 6B). Moreover, the cerebral cortex neurons in mice treated with BME, BDMF, and BBF showed normal histological structure ( Figure 6C-E, respectively). Additionally, a histopathological examination of the hippocampus subiculum was carried out, as shown in (Figure 7). The results demonstrated that the hippocampus subiculum of normal control mice and animals treated with BME, BDMF, and BBF had no pathological abnormalities ( Figure 7A,C-E, respectively). On the contrary, examined hippocampus subiculum sections from mice administered LPS revealed nuclear pyknosis and degeneration in some neurons ( Figure 7B). BME, BDMF, and BBF administration. Microscopical examination of the cerebral cortex ( Figure 6) revealed no histopathological alteration in normal control sections ( Figure 6A). While in mice injected with LPS, cerebral cortex sections had nuclear pyknosis and neuronal degeneration with focal gliosis ( Figure 6B). Moreover, the cerebral cortex neurons in mice treated with BME, BDMF, and BBF showed normal histological structure ( Figure  6C-E, respectively). Additionally, a histopathological examination of the hippocampus subiculum was carried out, as shown in (Figure 7). The results demonstrated that the hippocampus subiculum of normal control mice and animals treated with BME, BDMF, and BBF had no pathological abnormalities ( Figure 7A,C-E, respectively). On the contrary, examined hippocampus subiculum sections from mice administered LPS revealed nuclear pyknosis and degeneration in some neurons ( Figure 7B).  Microscopical examination of fascia dentata and hilus in the hippocampus (Figure 8) displayed no histopathological alteration in normal control sections, and the normal histological structure of the neurons was recorded in ( Figure 8A). Most of the hippocampus fascia dentata and hilus neurons of mice administered with LPS revealed nuclear pyknosis with degeneration ( Figure 8B). Regarding examined sections of mice treated with BME, BDMF, and BBF, there was no histopathological alteration when compared to the LPS-administered group ( Figure 8C-E, respectively).
Similarly, a microscopical investigation of the brain striatum was conducted in (Figure 9). Striatum sections of normal control mice revealed that the histological structure of neurons was normal and there was no histopathological alteration ( Figure 9A). On the other hand, sections from LPS-administered mice showed multiple focal eosinophilic plagues formation ( Figure 9B). Surprisingly, fine eosinophilic plagues formation was demonstrated in striatum sections of mice treated with BME ( Figure 9C), while examined sections of mice treated with BDMF showed the formation of eosinophilic plagues ( Figure 9D). Furthermore, striatum sections of mice treated with BBF revealed focal eosinophilic plagues formation ( Figure 9E). Ultimately, microscopical examination of the brain's cerebellum was exhibited in (Figure 10). The normal histological structure of neurons was observed in cerebellum sections of normal control and all other administered mice groups, with no histopathological alteration, as recorded in (Figure 10A-E, respectively). Microscopical examination of fascia dentata and hilus in the hippocampus (Figure 8) displayed no histopathological alteration in normal control sections, and the normal histological structure of the neurons was recorded in (Figure 8A). Most of the hippocampus fascia dentata and hilus neurons of mice administered with LPS revealed nuclear pyknosis with degeneration ( Figure 8B). Regarding examined sections of mice treated with BME, BDMF, and BBF, there was no histopathological alteration when compared to the LPSadministered group ( Figure 8C-E, respectively).
Similarly, a microscopical investigation of the brain striatum was conducted in (Figure 9). Striatum sections of normal control mice revealed that the histological structure of neurons was normal and there was no histopathological alteration ( Figure 9A). On the other hand, sections from LPS-administered mice showed multiple focal eosinophilic plagues formation ( Figure 9B). Surprisingly, fine eosinophilic plagues formation was demonstrated in striatum sections of mice treated with BME ( Figure 9C), while examined sections of mice treated with BDMF showed the formation of eosinophilic plagues ( Figure  9D). Furthermore, striatum sections of mice treated with BBF revealed focal eosinophilic plagues formation ( Figure 9E). Ultimately, microscopical examination of the brain's cerebellum was exhibited in (Figure 10). The normal histological structure of neurons was observed in cerebellum sections of normal control and all other administered mice groups, with no histopathological alteration, as recorded in (Figure 10A-E, respectively).
According to the above histopathological findings, both BME and BBF fractions effectively ameliorate LPS-induced AD disease in mice, which is consistent with biochemical analysis results.     Interestingly, LC-Mass findings in the current study revealed that BME was richer in phenolic constituents. The tentative identification of phenolic compounds such as baicalin, vitexin, caffeic acid, and protocatechuic acid in BME employing positive and negative ion modes may have a significant impact on the management of LPS-induced AD in mice. Baicalin can protect the blood-brain barrier (BBB) from LPS attacks. This action could be mediated by suppressing ROS production as well as the inflammatory response According to the above histopathological findings, both BME and BBF fractions effectively ameliorate LPS-induced AD disease in mice, which is consistent with biochemical analysis results.
Interestingly, LC-Mass findings in the current study revealed that BME was richer in phenolic constituents. The tentative identification of phenolic compounds such as baicalin, vitexin, caffeic acid, and protocatechuic acid in BME employing positive and negative ion modes may have a significant impact on the management of LPS-induced AD in mice. Baicalin can protect the blood-brain barrier (BBB) from LPS attacks. This action could be mediated by suppressing ROS production as well as the inflammatory response of BBB endothelial cells. So, baicalin has a good protective effect against LPS-induced brain disorders and may be employed as a clinical therapy option for AD [110].
Additionally, vitexin protected transgenic Caenorhabditis elegans nematode strains from Aβ proteotoxicity by acting as a neuroprotective agent by extending their life span. As a result, vitexin is a multifactorial agent that has the potential to be a promising therapeutic strategy in AD treatment [111]. Moreover, caffeic acid dramatically improved learning impairments and enhanced cognitive function in AD rats due to inhibition of oxidative stress and inflammation. In addition, compared to the AD model group, caffeic acid treatment caused a significant reduction in acetylcholinesterase efficacy and nitrite formation in the rats with AD [112].
Furthermore, protocatechuic acid (PA) exhibited protective benefits against cognitive impairment in an Aβ-induced AD mouse model. Treatment of higher PA was found to protect against cognitive impairment. Moreover, it had considerably lower lipid peroxidation and NO generation in the brain, kidney, and liver tissues [113].
Finally, the presence of these phenolic metabolites, which have previously been reported as neuroprotective agents, may explain the enhancement therapy of neurodegenerative disorders, particularly AD, observed in B. armeniaca leaves. In this regard, the existence of all of these bioactive metabolites may have a synergistic effect on Bunchosia extract, which is correlated to its powerful neuroprotective potential. Further bio-guided purification of active fractions of B. armeniaca extract is needed in the future to isolate and identify their major active principles.

Plant Material
Fresh B. armeniaca leaves have been collected from Mazhar Botanical Garden, Giza, Egypt, during March 2020, and have been generously authenticated by Agriculture Engineer Therease Labib, former Director of El-Orman Botanic Garden in Giza, Egypt, and Plant Taxonomy Consultant at the Agriculture Ministry. A vouchered specimen of B. armeniaca with serial number 24.3.2022 II was safely withheld at the Pharmacognosy Department, Faculty of Pharmacy, Cairo University.

Extraction and Fractionation
One and half kilogram of powdered dried B. armeniaca leaves were macerated in 90% methanol (7 liters) at ambient temperature. The extraction was repeated three times with addition of fresh solvent. The combined filtered extract was evaporated using a rotary vacuum evaporator at no more than 40 • C, yielding 150 g of dry residue. The crude methanol extract (BME) (140 g) was dissolved with 70 mL of distilled H 2 O. The aqueous solution was successively fractionated by dichloromethane and butanol saturated with water. After evaporation, dichloromethane (BDMF) (40 gm) and butanol (BBF) (45 gm) fractions were obtained. For biological studies and further analysis, the dried extract (10 g) and its fractions were stored at −20 • C.

Estimation of Total Phenolic and Total Flavonoid Contents
Total phenolic content was evaluated by Folin-Ciocalteu colorimetric technique [114]. A calibration curve was prepared using several concentrations of standard methanolic gallic acid solutions (1000, 800, 600, 400, 200, 100, and 50 µg/mL). A volume of 10 µL of extract solution (5 mg/mL methanol) or phenolic standard (1 mg/mL methanol) or blank was added to 100 µL of Folin Ciocalteu reagent and incubated for 5 min. After that, 80 µL of 1 M aqueous sodium carbonate was added and incubated at 37 • C for half an hour. Total phenols were quantified colorimetrically at 630 nm using a microplate reader, FluoStar Omega, in 6 replicates. A calibration curve (y = 0.0031x − 0.0564, R 2 = 0.9961) was created and data were given in microgram of gallic acid equivalents per milligram of crude extract (µg GA/mg extract).
The aluminum chloride (AlCl 3 ) method was developed by [115] to measure the total flavonoid content in the crude extract. For the calibration curve, standard rutin was utilized. One mg of rutin was dissolved in methanol at 1 mg/mL and then diluted to 1000, 600, 400, 200, 100, 50, and 10 µg/mL. The extract was prepared at a concentration of 5 mg/mL in methanol. The extract stock solution (0.5 mL) was added to methanol (1.5 mL), 1% AlCl 3 (0.1 mL), potassium acetate solution (0.1 mL, 1 M), and distilled H 2 O (2.8 mL). The resultant mixture was kept for incubation at a darkened room temperature for half an hour. The absorbance of the reaction was read at 420 nm by a microplate reader, FluoStar Omega, in 6 replicates. A calibration curve (y = 0.0032x + 0.0398, R 2 = 0.9981) was drawn and data were given in microgram of rutin equivalents per milligram of crude extract (µg RU/mg extract). For positive mode, solution (A) was composed of 5 mM ammonium formate (NH 4 HCO 2 ) in 1% MeOH using formic acid to adjust pH to 3.0, and the mobile phase of solution (B) was comprised of 100% ACN as the mobile phase. Whilst in negative mode, solution (C) was made up of 5 mM NH 4 HCO 2 in 1% MeOH using sodium hydroxide to adjust pH to 8. The following program was used to do the gradient elution: 10% B for 0-20 min; 90% B for 21-25 min; 10% B for 25.01-28 min; and then 90% B for column equilibration.

Mass Spectrophotometry
The mass spectrometry (MS) was carried out on a Triple TOF 5600+ system with a Duo-Spray source in the ESI mode (AB SCIEX, Concord, ON, Canada). In positive mode, the sprayer capillary and declustering potential voltages were 4500 and 80 eV, respectively, whereas in negative mode, they were −4500 and −80 V. The source temperature was adjusted to 600 • C, the curtain gas at 25 psi, and gases 1 and 2 at 40 psi. The collision energies of 35 V (positive mode) and −35 V (negative mode) were used, along with CE spreading of 20 V and an ion tolerance of 10 ppm. The information-dependent acquisition (IDA) technique was used to operate the TripleTOF5600+. Analyst-TF 1.7.1 was utilized to generate batches for MS and MS/MS data collection. The IDA approach was utilized to simultaneously obtain full-scan MS and MS/MS data. The approach used high-resolution survey spectra ranging from 50 to 1100 m/z, and the mass spectrometer was programmed to detect a 50-ms survey scan. Later, the top 15 intense ions were chosen for the acquisition of MS/MS fragmentation spectra upon every scan [116].
The MS-DIAL 3.70 open-source software was employed to conduct a non-targeting, small molecule comprehensive sample analysis [117]. ReSpect positive (2737 records) or ReSpect negative (1573 records) databases have been utilized as reference databases, depending on the acquisition technique. The search parameters for the collection of data were adjusted as MS1 and MS2 mass tolerance of 0.01 Da and 0.05 Da, while for detection of a peak, minimum peak height: 100 amplitude; mass slice width: 0.05 Da; smoothing level: 2 scans; minimum peak width: 6 scans; for identification, MS1 and MS2 tolerance: 0.2 Da/each; for alignment, retention time tolerance: 0.05 min, and MS1 tolerance of 0.25 Da.

DPPH Radical Scavenging Assay
The free radical potential of the extract, fractions, and standards (BME, BDMF, BBF, and Trolox) was determined spectrophotometrically utilizing the DPPH procedure described by [118]. In brief, 100 µL of fresh DPPH solution (0.1% in MeOH) and 100 µL of the dissolved methanolic sample were pipetted into 96 wells plate (n = 6). The reaction was gently mixed and kept for 30 min of incubation in the dark at ambient temperature. The resultant intensity reduction in DPPH color was determined at 540 nm before and after the reaction using a microplate reader, FluoStar Omega, at the termination of the incubation period, and data are represented as mean ± standard deviation (SD). The IC 50 values for the radical scavenging activities of the extract and fractions are expressed as 50% inhibition concentrations (µg/mL) of DPPH radicals in the reaction.

ABTS Cation Radical Decolorization Assay
The ABTS radical cation was determined spectrophotometrically using the procedure of [119] with slight changes to be performed in microplates. An ABTS solution was prepared by dissolving 192 mg of ABTS in distilled H 2 O and completing the volume to 50 mL with distilled H 2 O. One mL of the prepared ABTS solution was added to potassium persulfate (17 µL, 140 mM) and kept in the dark for one day. The final ABTS dilution was obtained by diluting the reaction mixture (1 mL) to MeOH (50 mL) to achieve an absorbance of 0.700 ± 0.030 at 734 nm. In a 96-microplate (n = 6), 190 µL of fresh ABTS solution was added to 10 µL of each sample (BME, BDMF, BBF), and the plate was kept for 2 h at a dark ambient temperature for incubation. At the termination of the incubation period, the intensity reduction in ABTS color was recorded at 734 nm before and after the reaction using a microplate reader, FluoStar Omega, and data are represented as mean ± SD. The IC 50 values for the radical scavenging activities of the extract and fractions are expressed as 50% inhibition concentrations (µg/mL) of the ABTS radicals in the reaction.

Ferric Reducing Antioxidant Power Assay
The FRAP technique was carried out in microplates with slight modifications as described by [120,121]. Briefly, a fresh FRAP solution was prepared shortly before the reaction in a 10:1:1 v/v/v ratio, consisting of acetate buffer (300 mM, PH = 3.6), tripyridyltriazine (TPTZ, 10 mM) in HCl (40 mM), and FeCl 3 (20 mM), respectively. In a 96-microplate (n = 6), 190 µL of fresh FRAP solution was added to 10 µL of each sample (BME, BDMF, BBF), and the plate was kept for 30 min of incubation at a dark ambient temperature. The resulting blue color was observed at 593 nm at the termination of the incubation period. The FRAP test measured the absorbance change generated by electron-donating antioxidants converting a colorless oxidized Fe +3 -TPTZ complex to a blue-colored Fe +2 molecule in acidic conditions. Data are represented as mean ± SD.

In Vitro Acetylcholinesterase Inhibition Activity
The inhibition of AChE activity was evaluated according to [122], which was modified by [123]. For the activity measurement, AChE was utilized as the enzyme, with acetylthiocholine iodide (AChI) as the substrate, 5,5-Dithiobis-(2-nitrobenzoic acid) (DTNB) as a coloring agent, and donepezil as a positive control. Briefly, sodium phosphate buffer (150 µL, 100 mM, PH = 8.0) was mixed with 10 µL of each sample (BME, BDMF, BBF) dissolved in methanol with a range of concentrations (1000 to 7.81 µg/mL). The AChE solution (20 µL, 5.32 × 10 −3 U) was then mixed and kept for 15 min of incubation at ambient temperature. Subsequently, DTNB (10 µL, 0.5 mM) and AChI (10 µL, 0.71 mM) were mixed for the initiation of the reaction. The hydrolysis of AChE can be evaluated by the determination of the colorful product 5-thio-2-nitrobenzoate anion formed by DTNB reaction with thiocholine, which is liberated by AChI hydrolysis. The absorbance of colored product was read at 412 nm using a Multiplate Reader.

Acute Toxicity Study
For this investigation, male and female Swiss mice weighing 20-30 gm were procured from the animal house laboratory at the National Research Centre (NRC), Cairo, Egypt. Mice were kept in a hygienic laboratory environment for seven days before the start of the biological experiment (adaptation period), held in a well-ventilated box at 22 ± 20 • C for a 12-h lighting and darkness cycle. A natural baseline diet was given to the mice. Diets and water were provided ad libitum, with water readily accessible. They were managed in compliance with animal experiment guidelines approved by the Ethical Committee of Medical Research, NRC, Cairo, Egypt.
Acute toxicity was carried out in compliance with the guidelines of the World Health Organization (WHO) for assessing the safety and efficacy of herbal remedies. Four groups of forty Swiss mice were created. Groups one and two: control male and female mice (ten for each group) were orally administered with saline. Groups three and four: male and female mice (ten for each group) were orally administered with a single dose of BME in graded doses up to 5 g/kg. The animals were observed daily for signs of behavioral changes for two weeks [124].

Evaluation of Anti-Alzheimer Activity Animals
Fifty male Swiss mice weighing 20-35 gm were chosen for the investigation. They were held in filter-top plastic cages under hygienic conditions, with 12-hour light and 12-h dark cycles and 50% humidity at 28 • C. Unnecessary disturbance of animals was avoided. Squeezing, pressure, and abrasive maneuvers were avoided when dealing with the animals. Throughout the experiment, mice were fed a standard pellet meal, and water was allowed ad libitum. The laboratory animal experiments were conducted to comply with the recommendations of the Guide for the Care and Use of Laboratory Animals of the National Research Centre, Egypt, and the National Institutes of Health (NIH No. 85
Effects on Y-Maze in LPS-Induced Alzheimer in Mice Y maze spontaneous alternation test was performed 24 h after the last treatment dose, according to [126]. An animal must remember which arm it has previously entered to be able to alternate its choice on a subsequent trial. Behavioral pharmacologists and others have praised spontaneous alternation behavior as a fast and comparatively basic memory test in recent years [127]. The experiment was conducted in a three-armed Y maze-shaped apparatus. A, B, or C were labeled on each arm. The test is divided into two parts, the first of which is the training phase. The mouse was permitted free movement throughout the maze for eight minutes. One day later, the mouse was allowed eight minutes of movements while its motion was recorded. The letter of the mouse was recorded down every time it entered an arm with all of its limbs inside. The alternations number refers to the successive entries into three separate arms in overlapping triplet sets (for example, ABCBACA = 3). Total arm entries are merely the total number of arms entered (for example, ABCBACA= 7). The alternation percentage was determined using the formula below [128]: Number of alternations/(Total arm entries − 2) * 100

Tissue Biochemical Analysis
Firstly, mice were anesthetized using an IP injection of ketamine at a dose of 80-100 mg/kg. After they achieved a deep anesthetic state, mice were sacrificed by decapitation. Each mouse's brain was promptly dissected and washed with phosphate-buffered saline (PBS) to eliminate extra blood. Weighed pieces were homogenized in PBS with the MPW-120 homogenizer, Med Instruments, Poland, to obtain a 20% homogenate, then they were kept overnight at −20 • C. Using a refrigerated centrifuge (laborzentrifugen, 2K15, Sigma, Osterode am Harz, Germany), the homogenates were spun for five minutes at 5000× g [129]. The supernatant was removed immediately and assayed for brain contents of NF-κB, IL-1β, CYP2E1, and GFAP. They were determined using an ELISA kit (SunLong Biotec Co., Ltd., Hangzhou, China).
Standards and samples were added to wells containing immobilized antibodies specific for rat NF-κB, IL-1β, CYP2E1, and GFAP and kept for 30 min of incubation at 37 • C. After washing, horseradish peroxidase-conjugated streptavidin was added to the wells, kept for 30 min of incubation at 37 • C, and then washed for a second time. The wells were filled with tetramethylbenzidine (TMB) substrate solution and kept for 15 min of incubation at 37 • C; a color was produced in proportion to the quantity of NF-κB, IL-1β, CYP2E1, and GFAP bound. A stop solution was added to the wells to terminate the color development, and color intensity was detected at 450 nm after 10 min [130].

Histopathological Examination
Autopsy specimens were obtained from mice brains, divided into several groups and preserved in 10% formol saline for a day. Subsequently, rinsing with tap water, dehydration was achieved by alcohol serial dilutions (methyl, ethyl, and absolute ethyl alcohol). Samples were cleaned with xylene and immersed in paraffin for a day at 56 • C in a hot air oven. Paraffin beeswax tissue blocks were made using a sledge microtome and sectioned at a thickness of 4 µM. The tissue sections were mounted on glass slides, deparaffinized, and stained with hematoxylin and eosin (H&E) for routine inspection under a light electric microscope [131].

Statistical Analysis
DPPH and ABTS assay data were estimated by Microsoft Excel ® . IC 50 value was computed by GraphPad Prism 5 ® through converting the concentrations to their logarithmic value and choosing a nonlinear inhibitor regression equation (log (inhibitor) vs. normalized response-variable slope equation) [132]. FRAP assay data were determined by Microsoft Excel ® . EC 50 value was computed by GraphPad Prism 5 ® using plotting log concentration values of the compound/extract against the normalized absorbances (as % maximum activity, where the greatest reading was normalized to be 100%). The EC 50 was calculated using a non-linear response regression equation (log (concentration) vs. normalized response-variable slope equation). AChE inhibition activity was performed in triplicate. Data were recorded as mean ± SD, and the results were expressed as an IC 50 value (µg/mL). Statistical analysis was conducted using GraphPad Prism (Ver. 5.0). In vivo study data are expressed as mean ± standard error (SE). One-way analysis of variance (ANOVA) was utilized for comparing various groups, followed by Fisher's LSD test for multiple comparisons. These statistical tests were carried out using GraphPad Prism software, version 5 (Inc., California, USA). The difference was considered significant when p < 0.05.

Conclusions
The current results highlight the possible use of Bunchosia armeniaca plant as a promising candidate for ameliorating AD. Methanolic extract of B. armeniaca leaves exerts a significant neuroprotective effect. Among its fractions, the butanol fraction demonstrated profound antioxidant and anti-Alzheimer properties, both in vitro, through inhibition of acetylcholinesterase, and in vivo, by improving behavioral alterations and enhancing several biochemical parameters. These activities are most likely due to the high content of polyphenols and flavonoids, as evidenced by metabolic profiling results. This work encourages further investigation of B. armeniaca leaves to explore its promising neuroprotective potential, to isolate its antioxidant components, and to assess their in vivo antioxidant activity in the future.  Informed Consent Statement: Not applicable.

Data Availability Statement:
The data presented in this study are available on request from the first or corresponding authors.

Conflicts of Interest:
The authors declare no conflict of interest.