Exogenous Postharvest Application of Calcium Chloride and Salicylic Acid to Maintain the Quality of Broccoli Florets

The importance of broccoli (Brassica oleracea var. italica) consumption has increased in recent years due to its significant amount of anticarcinogenic and antioxidant compounds, as well as its many vitamins. However, broccoli florets are a highly perishable product which rapidly senesce and turn yellow after harvest, resulting in losses in nutritional and bioactive compounds. Thus, in this study, we evaluated the effect of postharvest exogenous of salicylic acid (SA) and calcium chloride (CaCl2) and their combination on the quality of broccoli florets stored at 5 °C for 28 days to minimize the rapid senescence of broccoli florets. Samples treated with 2 mM SA alone or in combination with 2% CaCl2 showed lower weight loss and lower losses of chlorophyll content, vitamin C, phenolic compounds, carotenoids, flavonoids, and glucosinolates compared with the control samples. Additionally, antioxidant activity was maintained by either SA or SA + CaCl2 treatments while peroxidase activity was decreased. For higher quality and lower losses in antioxidant compounds of broccoli florets during refrigerated storage at 5 °C, SA + CaCl2 treatment could be helpful for up to 21 days.


Introduction
Broccoli (Brassica oleracea var. italica) belongs to the Brassicaceae family, and it contains considerable levels of vitamins and antioxidants such as chlorophyll pigments, phenolic

Plant Material, Treatments, and Storage Condition
Broccoli heads (cv. Imperial) were harvested at the commercial maturity stage (90 days from planting) from a private farm in Giza, Egypt, and transferred within three hours to the laboratory. Healthy heads, free from any defects or insect injuries, with high quality (compact and dark green) were selected for use in this experiment. Broccoli florets were randomly divided into four groups. Every group was immersed in the following solution for 15 min at room temperature (23° C): 2% calcium chloride, 2 mM salicylic acid (SA), 2% CaCl2 + 2 mM SA, and distilled water (control). Florets were allowed to dry at room temperature for one hour and packed in polyethylene trays (clamshells), then stored at 5 °C and 95% relative humidity for 28 days, as shown in Figure 1. The average weight of every tray was 220-230 g, and three replicates were used for each treatment. The following physical and chemical attributes were measured every 7 days.

Weight Loss, Appearance Score, Chlorophyll, and Carotenoids
Florets were weighted immediately after drying and at each sampling time to measure weight loss. The difference between initial weight and sample weight was used to calculate percentage weight loss according to Ali and Abdel-Aziz [27]. The sensory score (appearance) of broccoli florets was conducted by a panel consisting of four trained members (two women and two men) after each sampling time. A sensory score was recorded according to Goa et al. [28]. Appearance and visual quality were determined with a 5-1 scale, where 5 = extremely fresh and marketable (free from any color changes), 3 = fresh and marketable with a slight change in green color (minimum accepted quality), and 1 = moderate color change and unmarketable. To determine the content of chlorophyll and carotenoid in broccoli florets; 1 g of the sample was extracted in 10 mL of N,N-dimethylformamide for 48 h. Then, the extract was filtered by Whatman filter paper 1 and measured by the spectrophotometer at 470, 647, and 663 nm. The re-

Weight Loss, Appearance Score, Chlorophyll, and Carotenoids
Florets were weighted immediately after drying and at each sampling time to measure weight loss. The difference between initial weight and sample weight was used to calculate percentage weight loss according to Ali and Abdel-Aziz [27]. The sensory score (appearance) of broccoli florets was conducted by a panel consisting of four trained members (two women and two men) after each sampling time. A sensory score was recorded according to Goa et al. [28]. Appearance and visual quality were determined with a 5-1 scale, where 5 = extremely fresh and marketable (free from any color changes), 3 = fresh and marketable with a slight change in green color (minimum accepted quality), and 1 = moderate color change and unmarketable. To determine the content of chlorophyll and carotenoid in broccoli florets; 1 g of the sample was extracted in 10 mL of N,N-dimethylformamide for 48 h. Then, the extract was filtered by Whatman filter paper 1 and measured by the spectrophotometer at 470, 647, and 663 nm. The results were expressed as mg/g FW for chlorophyll content and mg/100 g FW for carotenoids according to Moran [29].

Vitamin C, Total Phenolic, and Flavonoids
Vitamin C content was determined in broccoli florets by the titration method according to the method described previously by Shehata et al. [30]. In brief, 10 g of raw material was homogenized for 10 min in 90 mL of oxalic acid (6%). Afterward, 25 mL of filtrated solution was titrated by 2,6-dichlorophenol indophenol, and the results were expressed as mg/100 g FW. Total phenolic was determined by using the Folin-Ciocalteu colorimetric method according to Singleton and Rossi [31]. In brief, ethanol solvent was used to extract total phenolic from fresh broccoli florets (5 g/100 mL) for 20 min. Then, a spectrophotometer (model UV-2401 PC, Shimadzu, Milano, Italy) was used to determine the content of phenolic compounds at 765 nm. The results were expressed as mg of gallic acid equivalent/100 g FW. To determine flavonoids in fresh broccoli florets, the method of El-Beltagi et al. [32] was followed.

Glucosinolates, Sulforaphane, and Peroxidase
Glucosinolates were determined according to the methods of Bjerg et al. [33] and Bjerg and Sorensen [34]. In brief, glucosinolate concentration was calculated by using glucobarbarin as an internal standard. Then, 0.2 g of sample was spiked with a 100 µL standard solution containing 5.0 µmol·mL −1 of sinigrin and glucobarbarin. To homogenize the previous mix, 5 mL of boiling methanol (70%) was used three times for 2 min using the Ultra-Turrax Homogenizer (Ika-Labortechnik, Staufen, Germany). Then, samples were centrifuged and concentrated to dryness in vacuo. The residue was dissolved in 2 mL of deionized water for HPLC analysis of glucosinolates.
Peroxidase activity was determined as described previously [4]. Briefly, 10 mL of extraction buffer (50 mM phosphate buffer, pH 7, containing 0.5 mM EDTA and 2% PVPP (w/v)) was used to grind broccoli samples. Then, the previous samples were centrifuged for 20 min at 21,925 rpm. The method of In et al. [37] was used to determine the peroxidase activity.

Antioxidant Activity (%) Using DPPH
DPPH antioxidant capacity (%) was measured according to Awad, et al. [38], with slight modification. In brief, 0.1 mL of previous ethanol broccoli extract was taken and mixed with 3.9 mL of DPPH solution. The absorbance of the sample and control (0.0024 mg/100 methanol DPPH solution) was measured at 520 nm after incubation in the dark at room temperature for 30 min using a spectrophotometer (model UV-2401 PC, Shimadzu, Milano, Italy). The result was calculated according to the following equation: antioxidant capacity % = [(absorbance of control − absorbance of sample)/absorbance of control × 1000].

Statistical Analysis
Data were subjected to analysis of variance using SPSS software (Ver. 20, SPSS Inc., Chicago, IL, USA). Means of different treatments were compared by the Duncan test at 5% (LSD). The values are presented as means with their standard error. Pearson's correlation test was performed by SPSS software. 5% (LSD). The values are presented as means with their standard error. Pearson's correlation test was performed by SPSS software.

Weight Loss, Appearance Score, Chlorophyll Content, and Carotenoids
Compared to the control, the broccoli florets treated with SA and CaCl2 or their combination were effective in reducing weight loss after 14 days until the end of refrigerated storage (Figure 2A). SA was more effective than CaCl 2 at reducing weight loss. After 28 days of storage, weight loss in the control was 21.86, 16.02, and 9.89% higher than it was in SA + CaCl 2 , SA, and CaCl 2 treatments, respectively. A negatively strong correlation was observed between weight loss and all tested parameters (except peroxidase activity) according to Pearson's coefficient correlation (Table 1). The appearance score rating of broccoli florets displayed a drastic decline after 14 days of storage until the end of the storage period ( Figures 2B and 3). However, the quality ranking of SA, CaCl 2 , and their combination treatments remained higher than the control. Control and CaCl 2 samples showed a limited acceptance quality (less than score 3) after 28 days of refrigerated storage.  A linear decrease in the chlorophyll content of broccoli florets was observed during the refrigerated storage for all treatments ( Figure 2C). There was no significant difference in chlorophyll content between treated broccoli florets and non-treated broccoli florets until 14 days of storage at 5 °C . However, after 21 and 28 days of the storage period, SA and SA + CaCl2 treatments suppressed the decrease of chlorophyll content compared with the control and CaCl2 treatments. After 28 days of storage, chlorophyll content in the control was 27.35, 16.66, and 5.56% lower than it was in SA + CaCl2, SA, and CaCl2 treatments, respectively. A positive correlation was observed between chlorophyll content and vitamin C, phenolic content, carotenoids, glucosinolates, and flavonoids, while a negative correlation was observed between chlorophyll content and peroxidase activity ( Table 1).
As shown in Figure 2D, a linear decrease in the carotenoids of broccoli florets was A linear decrease in the chlorophyll content of broccoli florets was observed during the refrigerated storage for all treatments ( Figure 2C). There was no significant difference in chlorophyll content between treated broccoli florets and non-treated broccoli florets until 14 days of storage at 5 • C. However, after 21 and 28 days of the storage period, SA and SA + CaCl 2 treatments suppressed the decrease of chlorophyll content compared with the control and CaCl 2 treatments. After 28 days of storage, chlorophyll content in the control was 27.35, 16.66, and 5.56% lower than it was in SA + CaCl 2 , SA, and CaCl 2 treatments, respectively. A positive correlation was observed between chlorophyll content and vitamin C, phenolic content, carotenoids, glucosinolates, and flavonoids, while a negative correlation was observed between chlorophyll content and peroxidase activity ( Table 1).
As shown in Figure 2D, a linear decrease in the carotenoids of broccoli florets was observed during the cold storage for all treatments. There was no significant difference in carotenoids content between all treatments until 7 days of cold storage. However, at 14 days of storage until the end of the storage time, carotenoids content was significantly higher in the broccoli florets treated with SA, CaCl 2 , and their combination than in the controls. At the end of the storage period, carotenoids in the control were 36.66, 33.72, and 20.83% lower than they were in the SA + CaCl 2 , SA, and CaCl 2 treatments, respectively.

Total Phenolic Compounds, Vitamin C, Flavonoids, and Glucosinolates
The content of total phenolic compounds in broccoli florets for all treatments showed a decrease from the beginning until 14 days of storage and then remained constant until 21 days and finally decreased ( Figure 4A). The total phenolic content in broccoli florets treated with SA and CaCl 2 + SA treatments was greater than in the control and CaCl 2 treatments. At the end of the storage time, the CaCl 2 + SA and SA treatments reduced the losses in total phenolic compounds by 27.50 and 15.94%, respectively, compared to the control treatment. A positive correlation between phenolic compounds and all parameters was recorded ( Table 1).
As presented in Figure 4B, all treatments showed a rapid decrease in vitamin C during the whole period of the cold storage; however, all treatments suppressed the losses of vitamin C compared with untreated broccoli florets. After 28 days of storage, vitamin C in the control was 33.87, 25.17, and 11.71% lower than it was in SA + CaCl 2 , SA, and CaCl 2 treatments, respectively. Our results showed a positive correlation between vitamin C and all other parameters, except peroxidase activity, which had a slight negative correlation (Table 1).
Flavonoids content decreased rapidly during the cold storage in all samples ( Figure 4C). Levels of flavonoids were similar in both SA and CaCl 2 + SA treatments during all storage periods and were higher than CaCl 2 and the control, respectively. After 28 days of cold storage, the flavonoid content of the broccoli florets treated with SA + CaCl 2 , SA, or CaCl 2 was 35.44%, 32.13%, or 20.78% higher than the control treatment. Glucosinolates decreased rapidly with storage time ( Figure 4D). However, all treatments showed significantly higher values of glucosinolates after 14 days until the end of storage time compared with the control. Samples treated with SA + CaCl 2 showed higher glucosinolate content than SA and CaCl 2 .

Total Phenolic Compounds, Vitamin C, Flavonoids, and Glucosinolates
The content of total phenolic compounds in broccoli florets for all treatments showed a decrease from the beginning until 14 days of storage and then remained constant until 21 days and finally decreased ( Figure 4A). The total phenolic content in broccoli florets treated with SA and CaCl2 + SA treatments was greater than in the control and CaCl2 treatments. At the end of the storage time, the CaCl2 + SA and SA treatments reduced the losses in total phenolic compounds by 27.50 and 15.94%, respectively, compared to the control treatment. A positive correlation between phenolic compounds and all parameters was recorded ( Table 1).
As presented in Figure 4B, all treatments showed a rapid decrease in vitamin C during the whole period of the cold storage; however, all treatments suppressed the losses of vitamin C compared with untreated broccoli florets. After 28 days of storage, vitamin C in the control was 33.87, 25.17, and 11.71% lower than it was in SA + CaCl2, SA, and CaCl2 treatments, respectively. Our results showed a positive correlation between vitamin C and all other parameters, except peroxidase activity, which had a slight negative correlation (Table 1).  Flavonoids content decreased rapidly during the cold storage in all samples ( Figure  4C). Levels of flavonoids were similar in both SA and CaCl2 + SA treatments during all storage periods and were higher than CaCl2 and the control, respectively. After 28 days of cold storage, the flavonoid content of the broccoli florets treated with SA + CaCl2, SA, or CaCl2 was 35.44%, 32.13%, or 20.78% higher than the control treatment. Glucosinolates decreased rapidly with storage time ( Figure 4D). However, all treatments showed significantly higher values of glucosinolates after 14 days until the end of storage time compared with the control. Samples treated with SA + CaCl2 showed higher glucosinolate content than SA and CaCl2.

Sulforaphane, Peroxidase Activity, and Antioxidant Activity
A linear decrease in the sulforaphane content in broccoli florets was recorded in all treatments during storage time. There was no significant difference between all treatments and the control ( Figure 5A). The sulforaphane contents in samples were decreased from 9.32 at zero time to 6.54 µg/g FW after 28 days of storage.
Peroxidase activity in all samples showed an increase from the beginning until 21 days of storage and then decreased ( Figure 5B). During the storage period, the highest peroxidase activity was observed in the control samples, followed by CaCl 2 treatment, while the SA + CaCl 2 and SA treatments showed the lowest peroxidase activities. Peroxidase activity correlated positively with vitamin C and carotenoids, according to Pearson's coefficient correlation (Table 1). Antioxidant activity was slightly increased after 7 days of storage and then decreased until the end of the storage period in all treatments ( Figure 5C). However, after 21 days of storage until the end of the storage, all treatments showed the highest antioxidant activity compared to the control. Antioxidant activity correlated positively with most other bioactive compounds (vitamin C, total phenolic compounds, flavonoids, and carotenoids), while it correlated negatively with weight loss according to Pearson's coefficient correlation (Table 1).
Plants 2022, 11, x FOR PEER REVIEW 10 of 17 ments and the control ( Figure 5A). The sulforaphane contents in samples were decreased from 9.32 at zero time to 6.54 µ g/g FW after 28 days of storage. Peroxidase activity in all samples showed an increase from the beginning until 21 days of storage and then decreased ( Figure 5B). During the storage period, the highest peroxidase activity was observed in the control samples, followed by CaCl2 treatment, while the SA + CaCl2 and SA treatments showed the lowest peroxidase activities. Peroxidase activity correlated positively with vitamin C and carotenoids, according to Pearson's coefficient correlation (Table 1). Antioxidant activity was slightly increased after 7 days of storage and then decreased until the end of the storage period in all treatments ( Figure 5C). However, after 21 days of storage until the end of the storage, all treatments showed the highest antioxidant activity compared to the control. Antioxidant activity correlated positively with most other bioactive compounds (vitamin C, total phenolic compounds, flavonoids, and carotenoids), while it correlated negatively with weight loss according to Pearson's coefficient correlation (Table 1).

Correlation Study
Pearson's correlation study (Table 1) and heatmap ( Figure 6) show the changes in bioactive properties of broccoli florets during storage were performed. Pearson's correlation study and heatmap were presented to further integrate and visualize the findings and data of our study. Significant and insignificant correlations are presented in Table 1. In the heatmap (Figure 6), all the measured parameters of treated broccoli florets during cold storage periods can be seen.

Correlation Study
Pearson's correlation study (Table 1) and heatmap ( Figure 6) show the changes in bioactive properties of broccoli florets during storage were performed. Pearson's correlation study and heatmap were presented to further integrate and visualize the findings and data of our study. Significant and insignificant correlations are presented in Table 1.
In the heatmap (Figure 6), all the measured parameters of treated broccoli florets during cold storage periods can be seen.

Weight Loss, Appearance Score, Chlorophyll Content, and Carotenoids
The most important factor determining the quality and marketable of horticulture crops is weight loss. Thus, all pre-and postharvest applications that decrease water loss lead to better quality. In this study and in a previous study [4], water loss increased with increasing storage time due to water evaporation, transpiration, and the respiration of

Weight Loss, Appearance Score, Chlorophyll Content, and Carotenoids
The most important factor determining the quality and marketable of horticulture crops is weight loss. Thus, all pre-and postharvest applications that decrease water loss lead to better quality. In this study and in a previous study [4], water loss increased with increasing storage time due to water evaporation, transpiration, and the respiration of broccoli florets [39]. Our results showed that either SA or CaCl 2 reduced weight loss during storage (Figure 2A). The postharvest application of SA has been used to reduce water loss in horticulture crops such as strawberries [40] and kiwifruits [41]. Reduced weight loss by SA treatment could be due to its inhibitory effects on ethylene biosynthesis [12], which leads to a decrease in respiration rate [42]. Additionally, SA could decrease weight loss by forcing stoma closure [43]. The CaCl 2 treatment can also reduce weight loss by decreasing the respiration rate [42]. Furthermore, the CaCl 2 treatment may promote the formation of calcium pectate hydrogel, which is responsible for water retention and cell toughness [44]. Additionally, Kazemi et al. [41] found that the SA + CaCl 2 dipping treatment decreased weight loss in kiwifruits during cold storage, which supports our hypothesis in this study.
Excellent appearance and deep green color (chlorophyll content) are the most important visual quality parameters of broccoli florets. Thus, yellowing reduces shelf-life and accelerates the senescence of broccoli florets. In this study, the SA application maintained the chlorophyll content, which could be due to its role in decreasing the respiration rate and ethylene biosynthesis [12] and delaying ripening and senescence [45]. Our results are in agreement with Wei et al. [46] and Turkyylmaz et al. [47], who found that SA application maintained the chlorophyll content of asparagus shoots and green beans. The role of CaCl 2 application for maintaining the appearance and chlorophyll content of broccoli florets could be due to its role in decreasing the respiration rate [48].
Carotenoids are the most abundant pigments in nature, with excellent antioxidant effects and the ability to treat chronic diseases in humans [49]. In this study, SA and CaCl 2 treatments maintained the level of carotenoids in broccoli florets during cold storage ( Figure 2D). Our results agree with Supapvanich and Promyou [15], who found that carotenoids in papaya fruit increased by 2 mM of SA treatment. Robert et al. [50] suggested that CaCl 2 application reduces the loss of carotenoids during cold storage via respiration reduction.

Total Phenolic Compounds, Vitamin C, Flavonoids, and Glucosinolates
Even under cold storage conditions, significant declines in phytochemicals content have been observed in broccoli florets after harvest [51]. SA is capable of maintaining bioactive components with antioxidant effects in horticultural commodities [52]. For example, the application of 1.0 mmol/L SA significantly reduced the loss of phenolic compounds in asparagus shoots [46]. In addition, SA application activated the biosynthesis of active compounds such as ascorbic acid, phenolic compounds, and flavonoid content [53][54][55][56]. The foliar application of 500 µM of SA maintained the chlorophylls, total carotenoids, and total phenolic contents of coriander [56]. In this study, the CaCl 2 treatment showed higher phenolic compounds than the control treatment, but the difference was not significant ( Figure 4A). However, the SA + CaCl 2 treatment preserved the phenolic compounds, demonstrating the importance of calcium application in preserving the phenolic compound. Ruiz et al. [57] mentioned the role of calcium in the activation of enzymes such as phenylalanine ammonia-lyase, polyphenol oxidase, and peroxidase, which are responsible for the metabolism of phenolics. In addition, Perucka and Olszówka [58] found that phenolic compounds were increased in lettuce by the application of 0.2 mol/L CaCl 2 .
Vitamin C is a natural antioxidant that could help to reduce the risk of cancer by scavenging reactive oxygen species (ROS) in the human body [59]. However, it is rapidly decreasing in fresh horticultural products due to a variety of circumstances, including refrigerated storage. As a result, it is important to maintain vitamin C content throughout the period of cold storage using an eco-friendly application. Our results showed that both individual SA and CaCl 2 or their combination were highly effective for preserving vitamin C in broccoli florets during cold storage ( Figure 4B). The same result has been recorded in previous work for treated strawberry fruit with the combination of SA and CaCl 2 [42]. In addition, SA treatment at a rate of 1.0 mmol/L SA was found to be a promising application in preventing vitamin C degradation in asparagus shoots [46]. The reduction in vitamin C loss by SA could be due to its role in activating ascorbate peroxidase activity, which results in a higher accumulation of vitamin C in fresh products [60]. Other research has suggested that high vitamin C contents by SA treatment could be related to the acceleration of biosynthetic dehydroascorbate [53]. The role of calcium in improving the quality of fresh fruits and vegetables has been established earlier [61]. The higher vitamin C level in broccoli florets could be related to the fact that calcium has a promoter effect on vitamin C content [62].
Flavonoids from plant sources, mainly in the skin of fruits, are classified as polyphenolic compounds which have the ability to scavenge free radicals [63]. A decline in flavonoids was observed during cold storage in all treatments ( Figure 4C). However, SA and CaCl 2 or their combination retarded this decline. Our results agree with Dokhanieh et al. [13], who found that treatment with 2 mM of SA significantly increased the accumulation of flavonoids in cornelian cherry fruits during refrigerated storage. SA has an influence on the photosynthetic rate, which could promote the biosynthesis and accumulation of carotenoids [64]. In this study, CaCl 2 treatment conserved flavonoids during cold storage compared with control samples ( Figure 4C). This, in turn, could be due to the effect of calcium application on stimulating the biosynthesis pathways of flavonoids [65].
The anticarcinogenic effects of glucosinolates in broccoli plants have been reported in several works, such as Fahey et al. [65]. In this study, both SA and CaCl 2 treatments conserved glucosinolates content in broccoli florets during cold storage ( Figure 4D). Previous work suggested that CaCl 2 enhanced glucosinolates biosynthesis, resulting in higher glucosinolates content [66]. Glucosinolates in broccoli florets were enhanced by SA, which could be due to the role of SA in delaying ripening and yellowing, which resulted in a lower loss in glucosinolates content.

Sulforaphane, Peroxidase Activity, and Antioxidant Activity
Our results indicated that sulforaphane was not changed by SA, CaCl 2 , or their combination. In contrast with our results, Zhuang et al. [67] found an increase in sulforaphane content in broccoli sprouts by foliar CaCl 2 application. The difference with this study could be explained by the difference in the method of CaCl 2 application or the difference in the content of sulforaphane in florets compared to sprouts. Further studies into the effects of SA and CaCl 2 on sulforaphane content in broccoli plants are required.
It has been reported that peroxidase activity enhances resistance in plant tissues, such as exposure to unfavorable conditions [68]. In our study, the reduction in peroxidase activity was observed in the SA and CaCl 2 applications ( Figure 5). Our results are in accordance with Lu et al. [69], who reported that peroxidase activity in pineapple fruit was suppressed by SA application under cold storage conditions. The reduction in peroxidase activity in the CaCl 2 treatment could be due to the role of calcium in reducing the respiration rate [48]. In addition, Guo et al. [70] found that peroxidase activity was decreased by SA + CaCl 2 + 6-Benzylaminopurine treatment in broccoli heads.
Our results in Figure 5B and previous work [25] indicated that antioxidant activities were increased by Ca treatment. In addition, our results are in agreement with Cui et al. [71], who found that the pre-harvest application of SA maintained a higher antioxidant level in apricot fruits compared with the control. Our results in Table 1 show a significant positive correlation between antioxidant activity and other compounds (chlorophyll content, vitamin C, phenolic compound, carotenoids, flavonoids, and glucosinolates). This, in turn, supported our hypothesis that SA maintained the antioxidant activity of broccoli florets by the accumulation of bioactive compounds.

Conclusions
The current study showed the effects of SA, Ca, and their combination treatments on broccoli florets' quality during refrigerated storage at 5 • C for 28 days. The use of SA + Ca combination treatments was more effective than using each treatment separately, while SA outperformed Ca. The postharvest SA + Ca treatment reduced weight loss while preserving the majority of bioactive components such as chlorophyll content, vitamin C, phenolic compound, carotenoids, flavonoids, and glucosinolates, as well as antioxidant activity (Figure 7). This postharvest treatment can be simply applied on a commercial scale to improve quality and increase the shelf life of broccoli florets.