Antiphotoaging Effects of Damiana (Turnera diffusa) Leaves Extract via Regulation AP-1 and Nrf2/ARE Signaling Pathways

Damiana (Turnera diffusa), of the family Passifloraceae, has been widely studied for its pharmacological effects, especially for antioxidant and antibacterial actions. However, there are limited scientific findings describing its antiphotoaging effects on the skin. In the present study, the underlying molecular mechanisms of the protective effect of Damiana were investigated in keratinocytes (HaCaTs) and normal human dermal fibroblasts (HDFs) subject to UVB irradiation. The mRNA expression of matrix metalloproteinases (MMPs) and procollagen type I was determined by reverse transcription-polymerase chain reaction. The protein expression of antiphotoaging-related signaling molecules in the activator protein-1 (AP-1) and nuclear factor erythroid 2-related factor 2 (NRF2)/antioxidant response element (ARE) pathways was assessed by Western blotting. We observed that Damiana blocked the upregulated production of reactive oxygen species induced in UVB-irradiated HaCaTs and HDFs in a dose-dependent manner. Treatment with Damiana also significantly ameliorated the mRNA expression of MMPs and procollagen type I. In addition, the phosphorylation level of c-Jun and c-Fos was also decreased through the attenuated expression of p-38, p-ERK, and p-JNK after treatment with Damiana. Furthermore, the treatment of cells with Damiana resulted in the inhibition of Smad-7 expression in the TGF-β/Smad pathway and upregulated the expression of the Nrf2/ARE signaling pathway. Hence, the synthesis of procollagen type I, a precursor of collagen I, was promoted. Collectively, these results provide us with the novel insight that Damiana is a potential source of antiphotoaging compounds.


Introduction
Physiological aging is a result of genetic causes; therefore, it is difficult to control. However, photoaging caused by environmental effects such as freckles and skin pigmentation due to ultraviolet rays can be controlled artificially.
Ultraviolet radiation can be classified by wavelength: UVA (315-400 nm), UVB (280-315 nm), and UVC (200-280 nm). In the case of UVB, approximately 90% is absorbed by the ozone layer, whereas the remaining 10% reaches the Earth's surface; thus, it has a greater impact than UVA [1]. UVB affects cells, producing reactive oxygen species (ROS), which induces oxidative stress and can accelerate skin aging in the human body [2].
Moreover, the direct exposure of keratinous cells to external stimuli, including sunlight, as well as continuous exposure to the external environment causes skin aging and oxidative stress. Such condition damages the extracellular matrix (ECM), collagen, and elastin, which provide cell structural and functional support. As shown in Figure 1, apigenin was identified in Damiana leaves extract at the concentration of 3.66 ± 0.19 mg/g.

Analysis of Chemical Contents of Damiana Leaves Extract
Damiana leaves extract contains a high content of total phenols and flavonoids represented as 101.5 ± 1.67 mg gallic acid/g extract and 108.8 ± 0.01 mg quercetin/g extract, respectively.
As shown in Figure 1, apigenin was identified in Damiana leaves extract at the concentration of 3.66 ± 0.19 mg/g.

Antioxidative Activities of Damiana Leaves Extract
To evaluate the antioxidative activity of the Damiana leaves, its radical scavenging effect was analyzed by DPPH and ABTS assays. As shown in Figure 2, the positive control, ascorbic acid, showed the scavenging effect on DPPH and ABTS radical with an IC50 value of 31.71 μg/mL and 82.69 μg/mL, respectively. Damiana leaves extract also significantly suppressed DPPH radicals with an IC50 value of 305.4 μg/mL and ABTS radicals with an IC50

Antioxidative Activities of Damiana Leaves Extract
To evaluate the antioxidative activity of the Damiana leaves, its radical scavenging effect was analyzed by DPPH and ABTS assays. As shown in Figure 2, the positive control, ascorbic acid, showed the scavenging effect on DPPH and ABTS radical with an IC 50 value of 31.71 µg/mL and 82.69 µg/mL, respectively. Damiana leaves extract also significantly suppressed DPPH radicals with an IC 50 value of 305.4 µg/mL and ABTS radicals with an IC 50 value of 381.2 µg/mL. These findings reveals that Damiana leaves exhibited a potent antioxidative effect.

Cytotoxicity of Damiana Leaves Extract
The effect of Damiana leaves on cell viability was investigated by MTT assay on Ha-CaT cells, as shown in Figure 3A. Damiana leaves noted with significant cytotoxicity at 100 μg/mL concentration. Additionally, the effect of Damiana leaves on cell viability was investigated by MTT assay on HDF cells. As shown in Figure 3B, the viabilities of irradiated cells were significantly decreased by 24.6% as compared to non-irradiated cells. Thus, further experiments were conducted on Damiana leaves at lower than 50 μg/mL. Data are presented as the mean ± SD. # and * indicate significant differences between the non-treated cells and induced groups, respectively. ## p < 0.01 vs. the non-treated group. * p < 0.05 and **p < 0.01 vs. the induced control.

Cytotoxicity of Damiana Leaves Extract
The effect of Damiana leaves on cell viability was investigated by MTT assay on HaCaT cells, as shown in Figure 3A. Damiana leaves noted with significant cytotoxicity at 100 µg/mL concentration. Additionally, the effect of Damiana leaves on cell viability was investigated by MTT assay on HDF cells. As shown in Figure 3B, the viabilities of irradiated cells were significantly decreased by 24.6% as compared to non-irradiated cells. Thus, further experiments were conducted on Damiana leaves at lower than 50 µg/mL.

Cytotoxicity of Damiana Leaves Extract
The effect of Damiana leaves on cell viability was investigated by MTT assay on Ha-CaT cells, as shown in Figure 3A. Damiana leaves noted with significant cytotoxicity at 100 μg/mL concentration. Additionally, the effect of Damiana leaves on cell viability was investigated by MTT assay on HDF cells. As shown in Figure 3B, the viabilities of irradiated cells were significantly decreased by 24.6% as compared to non-irradiated cells. Thus, further experiments were conducted on Damiana leaves at lower than 50 μg/mL. Data are presented as the mean ± SD. # and * indicate significant differences between the non-treated cells and induced groups, respectively. ## p < 0.01 vs. the non-treated group. * p < 0.05 and **p < 0.01 vs. the induced control. Data are presented as the mean ± SD. # and * indicate significant differences between the non-treated cells and induced groups, respectively. ## p < 0.01 vs. the non-treated group. * p < 0.05 and ** p < 0.01 vs. the induced control. To measure ROS levels in UVB-irradiated HaCaT and HDF cells were subjected to treatment with the fluorescence dye DCFH-DA. As shown in Figure 4A,B ROS levels were significantly increased by 44.6% and 45.9% in UVB-irradiated cells compared with non-irradiated cells. However, the Damiana leaves treated group showed a significant reduction in ROS compared with the UVB group. Moreover, compared to UVB control cells, treatment of 10 and 50 µg/mL Damiana leaves lowered ROS formation by 27.1% and 36.9%, 51.7% and 65.8%, respectively. It was recorded that Damiana leaves extract was more effective than the positive control ascorbic acid. On the other hand, ascorbic acid inhibited ROS levels by 36% and 50.1%. To measure ROS levels in UVB-irradiated HaCaT and HDF cells were subjected to treatment with the fluorescence dye DCFH-DA. As shown in Figure 4A, B ROS levels were significantly increased by 44.6% and 45.9% in UVB-irradiated cells compared with nonirradiated cells. However, the Damiana leaves treated group showed a significant reduction in ROS compared with the UVB group. Moreover, compared to UVB control cells, treatment of 10 and 50 μg/mL Damiana leaves lowered ROS formation by 27.1% and 36.9%, 51.7% and 65.8%, respectively. It was recorded that Damiana leaves extract was more effective than the positive control ascorbic acid. On the other hand, ascorbic acid inhibited ROS levels by 36% and 50.1%. After 24 h of treatment, intracellular ROS level was measured. The number of cells is plotted versus the dichlorofluorescein fluorescence detected by the FL-2 channel; results presented as histograms. Data are presented as the mean ± SD. # and * indicate significant differences from the non-irradiated control and UVB-treated groups, respectively. # p < 0.05 and ## p < 0.01 vs. the non-treated group. *, ** and *** p < 0.05, 0.01, and 0.001 vs. the UVB-treated control, respectively.   Similar to ELISA results, in irradiated control cells, the mRNA expression of MMP-1 was elevated by 23.5%, and collagen precursor procollagen type I expression was diminished by 29.3% as compared to the normal group. It was noted that Damiana leaves extract reduced UVB-induced MMP-1 expression by 28.7% (10 μg/mL) and 61.0% (50 μg/mL). Furthermore, Damiana leaves (50 μg/mL) promoted procollagen type I expression by 28.9% (10 μg/mL) and 58.5% (50 μg/mL), respectively, as compared with irradiated control cells. This is incongruent with the positive control ascorbic acid, which showed inhibition by 92.9% on MMP-1 level and upregulation by 43.7% on procollagen type I level, respectively ( Figure 6A,B).

Damiana Leaves
The mRNA expression of MMP-1 was elevated by 56.6%; meanwhile, collagen precursor procollagen type I expression was diminished by 76.1%, as compared to the normal group. It was recorded that Damiana leaves extract reduced UVB-induced MMP-1 expression by 74.8% (50 μg/mL). By contrast, Damiana leaves (50 μg/mL) promoted procollagen type I expression by 52.6% as compared with irradiated control cells. This result was comparable to the positive control ascorbic acid, which showed inhibition by 9.6% on MMP-1 level and upregulation by 43.5% on procollagen type I level, respectively ( Figure 6C,D). Data are presented as the mean ± SD. # and * indicate significant differences from the non-irradiated control and UVB-treated groups, respectively. # and ## p < 0.05 and 0.01 vs. the non-treated group, respectively. *, ** and *** p < 0.05, 0.01, and 0.001 vs.
the UVB-treated control, respectively.

Damiana Leaves Extract on the mRNA and Protein Expression of MMP-1 and Procollagen Type I in UVB-Irradiated HaCaT and HDF Cells
Similar to ELISA results, in irradiated control cells, the mRNA expression of MMP-1 was elevated by 23.5%, and collagen precursor procollagen type I expression was diminished by 29.3% as compared to the normal group. It was noted that Damiana leaves extract reduced UVB-induced MMP-1 expression by 28.7% (10 µg/mL) and 61.0% (50 µg/mL). Furthermore, Damiana leaves (50 µg/mL) promoted procollagen type I expression by 28.9% (10 µg/mL) and 58.5% (50 µg/mL), respectively, as compared with irradiated control cells. This is incongruent with the positive control ascorbic acid, which showed inhibition by 92.9% on MMP-1 level and upregulation by 43.7% on procollagen type I level, respectively ( Figure 6A,B).
The mRNA expression of MMP-1 was elevated by 56.6%; meanwhile, collagen precursor procollagen type I expression was diminished by 76.1%, as compared to the normal group. It was recorded that Damiana leaves extract reduced UVB-induced MMP-1 expression by 74.8% (50 µg/mL). By contrast, Damiana leaves (50 µg/mL) promoted procollagen type I expression by 52.6% as compared with irradiated control cells. This result was comparable to the positive control ascorbic acid, which showed inhibition by 9.6% on MMP-1 level and upregulation by 43.5% on procollagen type I level, respectively ( Figure  Data are presented as the mean ± SD. # and * indicate significant differences from the non-irradiated control and UVB-treated groups, respectively. ## and ### p < 0.01 and 0.001 vs. the non-treated group, respectively. *, ** and *** p < 0.05, 0.01, and 0.001 vs. the UVB-treated control, respectively.

Damiana Leaves Extract on MMP-1/Procollagen Type Ⅰ Activation in UVB-Irradiated HaCaT and HDF Cells
The cells were treated for 8 h with the indicated concentrations of Damiana leaves and treated with UVB (125 mJ/cm 2 ) and UVB (144 mJ/cm 2 ). As MMP-1 upregulation is a hallmark of photoaging, protein expression study also indicated an upregulation of MMP-1 by 72.6% which downregulated procollagen type I by 35.7%, compared to normal cells. However, treatment with Damiana leaves at 50 μg/mL reversed this trend, diminished MMP-1 expression by 93.4% as compared to the irradiated control group. Furthermore, Damiana leaves effectively promoted procollagen type I by 61.4% at 50 μg/mL ( Figure 7A, B).
As MMP-1 upregulation is a hallmark of photoaging, protein expression study also indicated an upregulation of MMP-1 by 72.9%, consequently downregulating procollagen type I by 25.1%, compared to normal cells. However, treatment with Damiana leaves at 50 μg/mL reversed this trend, diminished MMP-1 expression by 47.3%, compared to the irradiated control group. Furthermore, Damiana leaves effectively promoted procollagen type I by 56.4% at 50 μg/mL ( Figure 7C,D). Data are presented as the mean ± SD. # and * indicate significant differences from the non-irradiated control and UVB-treated groups, respectively. ## and ### p < 0.01 and 0.001 vs. the non-treated group, respectively. *, ** and *** p < 0.05, 0.01, and 0.001 vs. the UVB-treated control, respectively.

Damiana Leaves Extract on MMP-1/Procollagen Type I Activation in UVB-Irradiated HaCaT and HDF Cells
The cells were treated for 8 h with the indicated concentrations of Damiana leaves and treated with UVB (125 mJ/cm 2 ) and UVB (144 mJ/cm 2 ). As MMP-1 upregulation is a hallmark of photoaging, protein expression study also indicated an upregulation of MMP-1 by 72.6% which downregulated procollagen type I by 35.7%, compared to normal cells. However, treatment with Damiana leaves at 50 µg/mL reversed this trend, diminished MMP-1 expression by 93.4% as compared to the irradiated control group. Furthermore, Damiana leaves effectively promoted procollagen type I by 61.4% at 50 µg/mL ( Figure 7A,B). Data are presented as the mean ± SD. # and * indicate significant differences from the non-irradiated control and UVB-treated groups, respectively. ## and ### p < 0.01 and 0.001 vs. the non-treated group, respectively. *, ** and *** p < 0.05, 0.01, and 0.001 vs. the UVB-treated control, respectively.

Damiana Leaves Extract on MMP-1/Procollagen Type Ⅰ Activation in UVB-Irradiated HaCaT and HDF Cells
The cells were treated for 8 h with the indicated concentrations of Damiana leaves and treated with UVB (125 mJ/cm 2 ) and UVB (144 mJ/cm 2 ). As MMP-1 upregulation is a hallmark of photoaging, protein expression study also indicated an upregulation of MMP-1 by 72.6% which downregulated procollagen type I by 35.7%, compared to normal cells. However, treatment with Damiana leaves at 50 μg/mL reversed this trend, diminished MMP-1 expression by 93.4% as compared to the irradiated control group. Furthermore, Damiana leaves effectively promoted procollagen type I by 61.4% at 50 μg/mL ( Figure 7A, B).
As MMP-1 upregulation is a hallmark of photoaging, protein expression study also indicated an upregulation of MMP-1 by 72.9%, consequently downregulating procollagen type I by 25.1%, compared to normal cells. However, treatment with Damiana leaves at 50 μg/mL reversed this trend, diminished MMP-1 expression by 47.3%, compared to the irradiated control group. Furthermore, Damiana leaves effectively promoted procollagen type I by 56.4% at 50 μg/mL ( Figure 7C,D). Data are presented as the mean ± SD. # and * indicate significant differences from the non-irradiated control and UVB-treated groups, respectively. # and ### p < 0.05 and 0.001 vs. the non-treated group. *, ** and *** p < 0.05, 0.01, and 0.001 vs. the UVB-treated control, respectively.
As MMP-1 upregulation is a hallmark of photoaging, protein expression study also indicated an upregulation of MMP-1 by 72.9%, consequently downregulating procollagen  Figure 7C,D).
Under UVB (144 mJ/cm 2 ) irradiation, TGF-β1 was inhibited by 30% due to an increase in inhibitor Smad7 by 21.3%, compared to non-irradiated cells. Damiana leaves recovered the expression level of TGF-β1 by 42.9%, while inhibited Smad7 level by 94.5% at 50 μg/mL, compared to UVB-irradiated control group ( Figure 8C,D). p38, ERK, and JNK are MAPK subunits that can be phosphorylated by UVB irradiation [8]. To validate the Damiana leaves extract role in the mechanism of the MAPKs family, HaCaTs cells were irradiated, and upregulation or downregulation of MAPK subunits was quantified. As shown in Figure 9A,B, UVB triggered an elevation of activated p-ERK, p-JNK, and p-p38. However, treatment with Damiana leaves extracts reversed these changes in a concentration-dependent manner. A supplement of 50 μg/mL Damiana leaves extract suppressed expression of p-p38, p-ERK, and p-JNK by 83.7%, 12.1%, and 45.9%, respectively. Additionally, as shown in Figure 10A,B, UVB irradiation upregulated p-c-Fos and p-c-Jun. However, treatment of cells with 50 μg/mL Damiana leaves extract inhibited p-c-Fos and p-c-Jun levels by 25.7% and 42.6%, respectively.
The effect of Damiana leaves extract on MAPKs family members was studied in irradiated HDF cells. As shown in Figure 9C,D, UVB triggered an elevation of activated p-ERK, p-JNK, and p-p38. However, treatment with Damiana leaves extract reversed these changes in a concentration-dependent manner. A supplement of 50 μg/mL Damiana Under UVB (144 mJ/cm 2 ) irradiation, TGF-β1 was inhibited by 30% due to an increase in inhibitor Smad7 by 21.3%, compared to non-irradiated cells. Damiana leaves recovered the expression level of TGF-β1 by 42.9%, while inhibited Smad7 level by 94.5% at 50 µg/mL, compared to UVB-irradiated control group ( Figure 8C,D).

Damiana Leaves Extract on MAPK/AP-1 Activation in UVB-Irradiated HaCaT and HDF Cells
p38, ERK, and JNK are MAPK subunits that can be phosphorylated by UVB irradiation [8]. To validate the Damiana leaves extract role in the mechanism of the MAPKs family, HaCaTs cells were irradiated, and upregulation or downregulation of MAPK subunits was quantified. As shown in Figure 9A,B, UVB triggered an elevation of activated p-ERK, p-JNK, and p-p38. However, treatment with Damiana leaves extracts reversed these changes in a concentration-dependent manner. A supplement of 50 µg/mL Damiana leaves extract suppressed expression of p-p38, p-ERK, and p-JNK by 83.7%, 12.1%, and 45.9%, respectively. Additionally, as shown in Figure 10A leaves extract suppressed expression of p-p38, p-ERK, and p-JNK by 38.3%, 36.7% and 28.6%, respectively. To further investigate the mechanism of Damiana leaves, we measured protein expression of c-Fos, c-Jun, and their phosphorylated forms. As shown in Figure 10C, D, UVB irradiation upregulated p-c-Fos and p-c-Jun. However, treatment of cells with 50 μg/mL Damiana leaves extract inhibited p-c-Fos and p-c-Jun levels by 20.3% and 56.3%, respectively.  Data are presented as the mean ± SD. # and * indicate significant differences from the non-irradiated control and UVBtreated groups, respectively. # and ### p < 0.05 and 0.001 vs. the non-treated group, respectively. *, ** and *** p < 0.05, 0.01, and 0.001 vs. the UVB-treated control, respectively. leaves extract suppressed expression of p-p38, p-ERK, and p-JNK by 38.3%, 36.7% and 28.6%, respectively. To further investigate the mechanism of Damiana leaves, we measured protein expression of c-Fos, c-Jun, and their phosphorylated forms. As shown in Figure 10C, D, UVB irradiation upregulated p-c-Fos and p-c-Jun. However, treatment of cells with 50 μg/mL Damiana leaves extract inhibited p-c-Fos and p-c-Jun levels by 20.3% and 56.3%, respectively.  Data are presented as the mean ± SD. # and * indicate significant differences from the non-irradiated control and UVBtreated groups, respectively. # and ### p < 0.05 and 0.001 vs. the non-treated group, respectively. *, ** and *** p < 0.05, 0.01, and 0.001 vs. the UVB-treated control, respectively. Data are presented as the mean ± SD. # and * indicate significant differences from the non-irradiated control and UVB-treated groups, respectively. # and ### p < 0.05 and 0.001 vs. the non-treated group, respectively. *, ** and *** p < 0.05, 0.01, and 0.001 vs. the UVB-treated control, respectively.
The effect of Damiana leaves extract on MAPKs family members was studied in irradiated HDF cells. As shown in Figure 9C,D, UVB triggered an elevation of activated p-ERK, p-JNK, and p-p38. However, treatment with Damiana leaves extract reversed these changes in a concentration-dependent manner. A supplement of 50 µg/mL Damiana leaves extract suppressed expression of p-p38, p-ERK, and p-JNK by 38.3%, 36.7% and 28.6%, respectively. To further investigate the mechanism of Damiana leaves, we measured protein expression of c-Fos, c-Jun, and their phosphorylated forms. As shown in Figure 10C,D, UVB irradiation upregulated p-c-Fos and p-c-Jun. However, treatment of cells with 50 µg/mL Damiana leaves extract inhibited p-c-Fos and p-c-Jun levels by 20.3% and 56.3%, respectively.

Damiana Leaves Extract on Nrf2 Activation in UVB-Irradiated HaCaT and HDF Cells
To assess the antioxidant mechanism of Damiana leaves extract, we evaluated the expression of the antioxidant regulators Nrf2 and Nrf2-related antioxidant proteins in UVB-irradiated HaCaTs. As shown in Figure 11A,B, nuclear Nrf2 protein expression was increased by UVB stimulation. Treatment of cells with 50 µg/mL Damiana leaves extract accelerated the expression of Nrf2 protein by 18.6% compared with UVB irradiation. It was also found that the downregulation of Dihydrolipoamide dehydrogenase (DLD) by 57.8% from the irradiated HaCaT cells was reversed by Damiana leaves treatment by significantly upregulating DLD by 37.5%. Aside from the association of DLD to α-keto acid dehydrogenase, which is a regulator of ROS, it also plays a role in the metabolizing of α-lipoic acid, which activates two cytoprotective proteins, Nrf2 and HO-1. Moreover, HO-1 and NQO-1 levels were dramatically elevated by treatment with Damiana leaves as shown in Figure 11A,B. Furthermore, the expression of HO-1 and NQO-1 protein was increased by 66% and 56.3% with 50 µg/mL Damiana leaves treatment, respectively. UVB-irradiated HaCaTs. As shown in Figure 11A, B, nuclear Nrf2 protein expression was increased by UVB stimulation. Treatment of cells with 50 μg/mL Damiana leaves extract accelerated the expression of Nrf2 protein by 18.6% compared with UVB irradiation. It was also found that the downregulation of Dihydrolipoamide dehydrogenase (DLD) by 57.8% from the irradiated HaCaT cells was reversed by Damiana leaves treatment by significantly upregulating DLD by 37.5%. Aside from the association of DLD to α-keto acid dehydrogenase, which is a regulator of ROS, it also plays a role in the metabolizing of αlipoic acid, which activates two cytoprotective proteins, Nrf2 and HO-1. Moreover, HO-1 and NQO-1 levels were dramatically elevated by treatment with Damiana leaves as shown in Figure 11A, B. Furthermore, the expression of HO-1 and NQO-1 protein was increased by 66% and 56.3% with 50 μg/mL Damiana leaves treatment, respectively.
The Nrf2 and Nrf2-related antioxidant proteins in UVB-irradiated HDFs was also explored. As shown in Figure 11C, D, nuclear Nrf2 protein expression was increased by UVB stimulation. Treatment of cells with 50 μg/mL Damiana leaves extract resulted in the acceleration of the expression of Nrf2 protein by 54.3% compared with UVB irradiation. It was elucidated that Damiana leaves extract was able to significantly upregulate DLD by 60.2%. This result is a significant finding given that UVB-irradiated cells downregulated the production of DLD by 16.5%. DLD mechanism leads to the activation of cytoprotective proteins, Nrf2 and HO-1. Moreover, HO-1 and NQO-1 levels were dramatically elevated by treatment with Damiana leaves. As shown in Figure 11C, D, the expression of HO-1 and NQO-1 protein was increased by 58.8% and 32% with 50 μg/mL Damiana leaves treatment, respectively. Data are presented as the mean ± SD. # and * indicate significant differences from the non-irradiated control and UVB-treated groups, respectively. # and ## p < 0.05 and 0.01 vs. the non-treated group. *, ** and *** p < 0.05, 0.01, and 0.001 vs. the UVB-treated control, respectively.

Discussion
Thus, the molecular mechanisms of Damiana on HaCaTs and HDFs cells treated with UVB irradiation in vitro were elucidated. The results showed that Damiana leaves effectively inhibited UVB-induced photoaging by regulating the MMP-1/procollagen type I, Figure 11. Effect of Damiana leaves extract on protein expression Nrf2, DLD, HO-1, and NQO-1 signaling pathway on UVB-irradiated HaCaT (A,B) and HDF (C,D) cells. Data are presented as the mean ± SD. # and * indicate significant differences from the non-irradiated control and UVB-treated groups, respectively. # and ## p < 0.05 and 0.01 vs. the non-treated group. *, ** and *** p < 0.05, 0.01, and 0.001 vs. the UVB-treated control, respectively.
The Nrf2 and Nrf2-related antioxidant proteins in UVB-irradiated HDFs was also explored. As shown in Figure 11C,D, nuclear Nrf2 protein expression was increased by UVB stimulation. Treatment of cells with 50 µg/mL Damiana leaves extract resulted in the acceleration of the expression of Nrf2 protein by 54.3% compared with UVB irradiation. It was elucidated that Damiana leaves extract was able to significantly upregulate DLD by 60.2%. This result is a significant finding given that UVB-irradiated cells downregulated the production of DLD by 16.5%. DLD mechanism leads to the activation of cytoprotective proteins, Nrf2 and HO-1. Moreover, HO-1 and NQO-1 levels were dramatically elevated by treatment with Damiana leaves. As shown in Figure 11C,D, the expression of HO-1 and NQO-1 protein was increased by 58.8% and 32% with 50 µg/mL Damiana leaves treatment, respectively.

Discussion
Thus, the molecular mechanisms of Damiana on HaCaTs and HDFs cells treated with UVB irradiation in vitro were elucidated. The results showed that Damiana leaves effectively inhibited UVB-induced photoaging by regulating the MMP-1/procollagen type I, TGF-β1/Smad, and MAPK pathways related to skin aging. It was also revealed that Damiana leaves could inhibit the UVB-induced AP-1 mechanism and activate the Nrf2 pathway.
With regard to changes in ROS, ROS formation was decreased by 36.9% on HaCaTs ( Figure 4A) and 65.8% on HDFs in the group treated with 50 µg/mL Damiana leaves, which showed a significant 30% effectiveness compared with the positive control (ascorbic acid treatment) ( Figure 4B).
The mRNA expression of MMP-1 in HaCaTs was reduced by 61% in the 50 µg/mL treatment group compared with the control group, whereas the mRNA expression of procollagen type I was increased by 58.5%. Ascorbic acid, used as the control treatment, resulted in a 92.9% increase in procollagen type I (see Figure 6A,B). Lin P et al. and Li L et al. claimed that UVB-induced photoaging was reduced by suppressing ROS and MMP-1 [16,17]. In comparison, the results revealed that Damiana possesses potential antiaging properties, mediated through the inhibition of ROS and MMP-1 expression in UVB-irradiated HaCaTs and HDFs.
In addition, the mRNA expression of MMP-1 in HDFs was reduced by 74.8% through treatment with 50 µg/mL Damiana leaves compared with the control group. Furthermore, mRNA expression of procollagen type I was lower in the control group than in the Damiana leaves treatment groups, where a 52.6% increase was noted at 50 µg/mL ( Figure 6C,D). These results were comparable to the positive control (ascorbic acid), which resulted in 9.6% inhibition of MMP-1 expression and 43.5% upregulation of procollagen type I expression. Therefore, it can be seen that Damiana leaves exerted a protective effect on collagen expression, by increasing procollagen type I mRNA, which was reduced by UVB which also resulted in a reduction in MMP-1 expression. Wei Gao et al. and Yu Shuai et al. reported that UVB-induced MMP production promotes procollagen type I in HaCaTs and HDFs [18,19]. Damiana leaves were observed to be more efficient in this study; the treatment of HaCaTs with 50 µg/mL Damiana leaves extract increased the protein expression of Nrf2 protein by 18.6% compared with UVB irradiation.
In addition, HO-1 and NQO-1 levels were dramatically elevated by Damiana leaves treatment. As shown in Figure 11A,B, the expression of HO-1 and NQO-1 proteins significantly increased by 66% and 56.3% with treatment at 50 µg/mL. Similarly, in HDFs, treatment of the cells with 50 µg/mL Damiana leaves extract increased the expression of Nrf2 protein by 54.3% compared to UVB irradiation. Furthermore, the protein expression of HO-1 and NQO-1 increased by 58.8% and 32%, respectively, in the 50 µg/mL Damiana leaves treatment group (see Figure 11C,D).
To further investigate the mechanism of Damiana leaves, we measured the protein expression of c-Fos, c-Jun, and their phosphorylated forms and stronger effects on the AP-1 and Nrf2/ARE pathways were found for Damiana treatment than for the positive control. First, in the AP-1 signaling pathway, p-c-Fos and p-c-Jun decreased by 14.96% and 38.42%, respectively, compared with ascorbic acid with a 50 µg/mL treatment concentration of Damiana leaves in HaCaTs, and decreased by 1.89% and 26.51%, respectively, in HDFs. Second, in the Nrf2/ARE signaling pathway, Nrf2 decreased by 27.13% compared to ascorbic acid at a 50 µg/mL treatment concentration of Damiana leaves, and then HO-1 and NQO-1 also decreased by 38.89% and 17.31%, respectively, in HaCaTs. It was also found that Nrf2 decreased by 29.39% and HO-1 and NQO-1 decreased by 36.57% and 32.28%, respectively, in HDFs. Seo SA et al. and Wang YS et al. reported that UVB-induced damage occurred through the regulation of the AP-1 and Nrf2/ARE pathways in HaCaTs and HDFs [19,20]. Compared with the above studies, it was confirmed that Damiana leaves have excellent efficacy on the AP-1 and Nrf2/ARE mechanism.
In this study, it was confirmed that Damiana leaves extract reduced the AP-1 signaling activity, and upregulated the expression of Nrf2/ARE signaling antioxidant enzymes, which inhibit cell damage from ROS generation, and thereby protect HaCaTs and HDFs from photoaging and cell damage (see Figure 12). The findings in this study, show that Damiana has significant potential as a biotic molecule that can be utilized in the treatment of photoaging. This study presents the discovery of a novel pharmacological potential of Damiana.

Sample Preparation
A total 100 g Damiana leaves was extracted in 500 mL of 70% ethanol and constantly shaken for 24 h by a Twist shaker at room temperature. The extraction procedure was done thrice. The extracts were collected and subsequently filtered using filter paper (Whatman, Maidstone, Knent, UK). Then, the sample was concentrated by rotary vacuum evaporation (EYELA WORLD-Tokyo Rikakikai Co., LTD., Tokyo, Japan) at 40 °C. The resulted extract yielded 7.38%.

Total Phenolic and Flavonoid Contents
The total phenolic content of Damiana leaves extract was examined based on Folin-Ciocalteu colorimetric method [5]. Briefly, either gallic acid (6.25-100 μg/mL) standard or plant extract was reacted with 1M Folin-Ciocalteu reagent for 15 min. Then, 0.7 M sodium carbonate in NaOH was added, and the mixture was incubated for 1 h. The absorbance value was measured at a wavelength of 625 nm.
The total flavonoid content of Damiana leaves extract was quantified based on aluminum chloride colorimetric method [6]. First, 50 mg/mL sodium nitrate was mixed with either standard quercetin (0.03125-1 mg/mL) or plant extract. After incubation for 5 min, aluminum chloride was reacted with the mixture for an additional 6 min. Finally, 1M sodium The findings in this study, show that Damiana has significant potential as a biotic molecule that can be utilized in the treatment of photoaging. This study presents the discovery of a novel pharmacological potential of Damiana.

Sample Preparation
A total 100 g Damiana leaves was extracted in 500 mL of 70% ethanol and constantly shaken for 24 h by a Twist shaker at room temperature. The extraction procedure was done thrice. The extracts were collected and subsequently filtered using filter paper (Whatman, Maidstone, Knent, UK). Then, the sample was concentrated by rotary vacuum evaporation (EYELA WORLD-Tokyo Rikakikai Co., LTD., Tokyo, Japan) at 40 • C. The resulted extract yielded 7.38%.

Total Phenolic and Flavonoid Contents
The total phenolic content of Damiana leaves extract was examined based on Folin-Ciocalteu colorimetric method [5]. Briefly, either gallic acid (6.25-100 µg/mL) standard or plant extract was reacted with 1M Folin-Ciocalteu reagent for 15 min. Then, 0.7 M sodium carbonate in NaOH was added, and the mixture was incubated for 1 h. The absorbance value was measured at a wavelength of 625 nm.
The total flavonoid content of Damiana leaves extract was quantified based on aluminum chloride colorimetric method [6]. First, 50 mg/mL sodium nitrate was mixed with either standard quercetin (0.03125-1 mg/mL) or plant extract. After incubation for 5 min, aluminum chloride was reacted with the mixture for an additional 6 min. Finally, 1M sodium hydroxide was added and incubated for 40 min. Optical density was determined at a wavelength of 450 nm.
The measurement was performed by a microplate reader (Molecular Devices FilterMax F5; San Francisco, CA, USA). The total phenols and flavonoids were presented as gallic acid and quercetin equivalents in mg per gram of plant extract, respectively.

2,2-Diphenyl-1-Picrylhdrazyl Radical Scavenging Activity
The antioxidant of Damiana leaves extract on 2,2-diphenyl-1-picrylhdrazyl (DPPH, PubChem CID: 2375032) was examined. Various concentrations of Damiana leaves (31.25 -1000 µg/mL) were also tested. Ascorbic acid was used as the positive control [7]. The 0.2 mM DPPH in 100% methanol solution was prepared. An aliquot of 40 µL sample was reacted with 160 µL of DPPH solution, followed by dark incubation at 37 • C for 30 min. The optical density was determined at a wavelength of 595 nm. The inhibitory effect of the sample was assessed using the following Equation: OD 0 : Optical density of negative control; OD x : Optical density of the sample.

2,2 -Azino-Bis (3-Ethylbenzothiazoline-6-Sulfonic Acid) (ABTS) Radical Scavenging Activity
The antioxidant effect of Damiana leaves extract on ABTS (ABTS, PubChem CID: 5464076) was detected. Various concentrations of Damiana leaves (31.25-1000 µg/mL) were also evaluated. Ascorbic acid was used as a positive control. A solution of ABTS was made from the reaction of a 2.5 mM ABTS solution with 1 mM 2,2 -azobis(2-amidinopropane) dihydrochloride (AAPH) and 150 mM sodium chloride. Then, the solution was incubated at 70 • C for 30 min. In each well of a 96-well plate, an aliquot of 4 µL sample was reacted with 196 µL of ABTS solution, followed by dark incubation at 37 • C for 10 min. The optical density was determined at a wavelength of 405 nm. The inhibitory effect of the sample was assessed using the following Equation: ABTS radical inhibition (%) = OD 0 : Optical density of negative control; OD x : Optical density of the sample.

Cell Culture and Treatment
Murine macrophage Raw264.7 cells were provided by Korean Cell Bank (Seoul, Korea). Human keratinocyte (HaCaTs) by Korean Cell Bank (Seoul, Korea) and Normal Adult Human Primary Dermal Fibroblasts (HDFs) (ATCC PCS-201-012) were purchased from ATCC (Manassas, VA, USA). The cells were grown in an incubator at 37 • C under a humidified atmosphere containing 5% CO 2 . DMEM medium supplemented with 10% heat-inactivated FBS, 1% antibiotics, and antimycotic solution was used for cell culture.
To induce inflammatory responses, Raw264.7 cells were sensitized with 1 µg/mL LPS at the cell confluence of 80% for 24 h. An aliquot of 10 µM dexamethasone (positive control) or Damiana leaves extract (1-50 µg/mL) was diluted in serum-free medium and supplemented at the same time of LPS treatment.

MTT Assay
After 24 h of treatment with lipopolysaccharide (LPS) or 72 h of treatment with UVB, 1 mg/mL MTT was added to the cell culture and then incubated for 3 h. After incubation, the medium was discarded, followed by the addition of DMSO to solubilize formazan. The optical density was recorded at a wavelength of 595 nm.

NO Assay
NO production was measured in LPS-induced Raw264.7 cells. Raw264.7 cells were seeded at the density of 1 × 10 6 cells/mL in 96-well cell culture plates (SPL Life Sciences Co., Ltd., Gyeonggi, Korea) and were incubated for 24 h. Then, 24 h after LPS sensation, the secretion of NO was quantified in the cell culture supernatant. A volume of 100 µL of cell culture supernatant was reacted with 100 µL Griess reagent, a mixture of 1% sulfanilamide in 5% phosphoric acid and 0.1% N-(1-Naphthyl) ethylenediamine dihydrochloride (1:1 ratio). Then, the plate was incubated for 10 min at 37 • C. The absorbance density was measured at 595 nm.

ROS Assay
Intracellular ROS levels were measured in UVB-exposed HaCaT and HDF cells. After 24 h of sample treatment and sensitizer exposure, the supernatant was discarded, and the cells were incubated with 30 µM 2 7 -dichlorofluorescein diacetate (DCFH-DA) (Sigma-Aldrich, St. Louis, MO, USA) for 30 min at 37 • C under the dark condition. Then, the cells were rinsed two times with cooled 1X PBS and collected by using 0.25% and 0.05% trypsin EDTA. Quantitation of intracellular ROS was evaluated by a BD Accuri C6 flow cytometer system (BD Accuri C6, Ann Arbor, MI, USA). The data were collected and analyzed by using FCS 6 plus Research Edition software.

Enzyme-Linked Immunosorbent Assay
HaCaT and HDF cells were seeded at the density of 1.5 × 10 5 cells/mL in 35 mm cell culture plates to acquire treatment conditions after 24 h. After 72 h of UVB irradiation, cell supernatant was collected and measured [7].
The concentrations of MMP-1 and MMP-3 protein in media were estimated using commercially available ELISA kits following the manufacturers' instructions. Each sample was repeatedly analyzed twice.

Reverse Transcriptase (RT)-PCR
Cells were collected 24 h after sensitization with inducers. RNA was isolated using TRIZOL reagent following the manufacturer guidelines (Invitrogen Life Technologies, Carlsbad, CA, USA). An equal amount of RNA (3 µg) was reverse transcribed using PCR premix (Bioneer Co., Daejeon, Korea), 0.5 µg/mL oligo-(dT)15 primer, and 0.5 µg/mL hexamer primer. The cDNA was resynthesized at 42 • C for 60 min and was incubated at 94 • C for 5 min to stop the reaction. Amplified products were observed by gel electrophoresis and detected by nucleic acid staining (NobleBio Inc., Gyeonggi, Korea) under UV illumination. GAPDH was used for normalization.

Western Blot
Cell lysates were merged in RIPA buffer (Sigma-Aldrich, St. Louis, MO, USA) for at least 1 h and centrifuged at 12,000 rpm in 15 min to obtain total protein extract. Protein concentration was calibrated using Bradford reagent (Bio-Rad, Hercules, CA, USA). Homogenized proteins were separated by SDS-PAGE and transferred to a nitrocellulose membrane (Bio-rad). Transfer membranes were blocked in 5% skim milk or 5% BSA for 30 min. After several washing steps with 1X TBST, the primary membrane was added with primary antibodies overnight at 4 • C. Subsequently, after incubation with secondary antibody for 1 h, the protein bands were detected using chemiluminescence detection ECL reagents (Fujifilm, LAS-4000, Tokyo, Japan) and ImageMaster™ 17 2D Elite software, version 3.1 (Amersham Pharmacia Biotech, Piscataway, NJ, USA). β-actin and histone were used for normalization of either total protein extract or nuclear protein extract, respectively.

Statistical Analysis
The data were analyzed using Statistical Analysis System (GraphPad Prism 5). All experiments were conducted with three replications. Data are shown as mean ± standard deviation (SD). Significant differences between different treatments were analyzed using a one-way analysis of variance followed by Duncan's test. The comparison between sample treatments and the control group was performed by using Student's t-tests. Statistical significance was set as follows: * p < 0.05, ** p < 0.01, and *** p < 0.001. Funding: This work was supported by the Snow White Factory Co., Ltd.

Data Availability Statement:
The data presented in this study are available in the main text.