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Article

The Effect of Cold Stress on the Root-Specific Lipidome of Two Wheat Varieties with Contrasting Cold Tolerance

1
Biotechnology Research Institute, Universiti Malaysia Sabah, Jalan Universiti, Kota Kinabalu 88400, Malaysia
2
School of Bio Sciences, The University of Melbourne, Parkville, VIC 3010, Australia
3
Protein Chemistry and Metabolism Unit, St. Vincent’s Institute of Medical Research, Fitzroy, VIC 3065, Australia
4
Max-Planck-Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Potsdam, Germany
5
Advanced Genomics Division, Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, Australia
6
SCIEX, Mulgrave, VIC 3170, Australia
7
CSIRO Agriculture & Food, GPO Box 1700, Canberra, ACT 2601, Australia
8
Research School of Biology, The Australian National University, Acton, ACT 2601, Australia
*
Author to whom correspondence should be addressed.
Academic Editors: Alice Pajoro and Julia Qüesta
Plants 2022, 11(10), 1364; https://doi.org/10.3390/plants11101364
Received: 7 April 2022 / Revised: 1 May 2022 / Accepted: 13 May 2022 / Published: 20 May 2022
(This article belongs to the Special Issue Molecular Responses to Temperature in Plants)
Complex glycerolipidome analysis of wheat upon low temperature stress has been reported for above-ground tissues only. There are no reports on the effects of cold stress on the root lipidome nor on tissue-specific responses of cold stress wheat roots. This study aims to investigate the changes of lipid profiles in the different developmental zones of the seedling roots of two wheat varieties with contrasting cold tolerance exposed to chilling and freezing temperatures. We analyzed 273 lipid species derived from 21 lipid classes using a targeted profiling approach based on MS/MS data acquired from schedule parallel reaction monitoring assays. For both the tolerant Young and sensitive Wyalkatchem species, cold stress increased the phosphatidylcholine and phosphatidylethanolamine compositions, but decreased the monohexosyl ceramide compositions in the root zones. We show that the difference between the two varieties with contrasting cold tolerance could be attributed to the change in the individual lipid species, rather than the fluctuation of the whole lipid classes. The outcomes gained from this study may advance our understanding of the mechanisms of wheat adaptation to cold and contribute to wheat breeding for the improvement of cold-tolerance. View Full-Text
Keywords: chilling; freezing; lc-tandem-ms; lipidome; spatial root; wheat; young; wyalkatchem chilling; freezing; lc-tandem-ms; lipidome; spatial root; wheat; young; wyalkatchem
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MDPI and ACS Style

Cheong, B.E.; Yu, D.; Martinez-Seidel, F.; Ho, W.W.H.; Rupasinghe, T.W.T.; Dolferus, R.; Roessner, U. The Effect of Cold Stress on the Root-Specific Lipidome of Two Wheat Varieties with Contrasting Cold Tolerance. Plants 2022, 11, 1364. https://doi.org/10.3390/plants11101364

AMA Style

Cheong BE, Yu D, Martinez-Seidel F, Ho WWH, Rupasinghe TWT, Dolferus R, Roessner U. The Effect of Cold Stress on the Root-Specific Lipidome of Two Wheat Varieties with Contrasting Cold Tolerance. Plants. 2022; 11(10):1364. https://doi.org/10.3390/plants11101364

Chicago/Turabian Style

Cheong, Bo E., Dingyi Yu, Federico Martinez-Seidel, William W.H. Ho, Thusitha W.T. Rupasinghe, Rudy Dolferus, and Ute Roessner. 2022. "The Effect of Cold Stress on the Root-Specific Lipidome of Two Wheat Varieties with Contrasting Cold Tolerance" Plants 11, no. 10: 1364. https://doi.org/10.3390/plants11101364

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