Antidiarrheal and Cardio-Depressant Effects of Himalaiella heteromalla (D.Don) Raab-Straube: In Vitro, In Vivo, and In Silico Studies

Himalaiella heteromalla (D.Don) Raab-Straube is a commonly used remedy against various diseases. Crude extract and fractions of H. heteromalla were investigated for a gastrointestinal, bronchodilator, cardiovascular, and anti-inflammatory activities. H. heteromalla crude extract (Hh.Cr) relaxed spontaneous contractions and K+ (80 mM)-induced contraction in jejunum tissue dose-dependently. The relaxation of K+ (80 mM) indicates the presence of Ca++ channel blocking (CCB) effect, which was further confirmed by constructing calcium response curves (CRCs) as they caused rightward parallel shift of CRCs in a manner comparable to verapamil, so the spasmolytic effect of Hh.Cr was due to its CCB activity. Application of Hh.Cr on CCh (1 µM) and K+ (80 mM)-induced contraction in tracheal preparation resulted in complete relaxation, showing its bronchodilator effect mediated through Ca++ channels and cholinergic antagonist activity. Application of Hh.Cr on aortic preparations exhibited vasorelaxant activity through angiotensin and α-adrenergic receptors blockage. It also showed the cardio suppressant effect with negative chronotropic and inotropic response in paired atrium preparation. Similar effects were observed in in vivo models, i.e., decreased propulsive movement, wet feces, and inhibition of edema formation.

Traditionally, H. heteromalla is used in herbal products to treat fever, menstruation, circulation, pain, and rheumatic arthritis [7].It is used in wounds, cuts, and fever [8].The leaf paste and mustard oil mixture are used for wounds and leukoderma.It has carminative property, used for coeliac diseases [9,10].It is used to remedy burning parts of the body, menstrual problems, piles, psoriasis, rheumatoid arthritis, cardiotonic cough with cold, and altitude sickness, and provide anticancer and anti-fatigue actions [11].It is used as an anti-inflammatory and prevents ischemic stroke [3].Therefore, H. heteromalla was investigated in in vitro, in vivo, and in silico models as a possible tool to treat gastrointestinal, cardiovascular, respiratory, and inflammatory ailments.

Phytochemical Analysis of Himalaiella heteromalla
The preliminary phytochemical analysis of Hh.Cr confirmed the presence of glycosides, saponins, alkaloids, and flavonoids.

HPLC Separation of Phenolic Acids and Flavonoids
The separation factor and resolution of all separated compounds were >1.0 and >1.5, respectively.The reproducibility of separate components was also good with RSD < 2% (run to run) and 2.7% (day to day) [12].The HPLC chromatograms for the identified phenolic and flavonoid compounds are shown in Figure 1, and Table 1 presents the phenolic and flavonoid compounds identified in Hh.Cr.The most abundant phenolic compounds were gallic acid (184.98 µg/g), hydroxybenzoic acid (6.8 µg/g), and vanillic acid (8.1 µg/g); while the identified flavonoid compound was catechin (160.37 µg/g).
cold, and altitude sickness, and provide anticancer and anti-fatigue actions [11].It is used as an anti-inflammatory and prevents ischemic stroke [3].Therefore, H. heteromalla was investigated in in vitro, in vivo, and in silico models as a possible tool to treat gastrointestinal, cardiovascular, respiratory, and inflammatory ailments.

Phytochemical Analysis of Himalaiella heteromalla
The preliminary phytochemical analysis of Hh.Cr confirmed the presence of glycosides, saponins, alkaloids, and flavonoids.

HPLC Separation of Phenolic Acids and Flavonoids
The separation factor and resolution of all separated compounds were >1.0 and >1.5, respectively.The reproducibility of separate components was also good with RSD < 2% (run to run) and 2.7% (day to day) [12].The HPLC chromatograms for the identified phenolic and flavonoid compounds are shown in Figure 1, and Table 1 presents the phenolic and flavonoid compounds identified in Hh.Cr.The most abundant phenolic compounds were gallic acid (184.98 μg/g), hydroxybenzoic acid (6.8 μg/g), and vanillic acid (8.1 μg/g); while the identified flavonoid compound was catechin (160.37 μg/g).

Effect on Jejunum Preparations
The Hh.Cr and its ethyl acetate (Hh.Ea) fraction showed the relaxant effect on exposure to the rhythmic contraction of jejunum preparations in organ bath within con-

Antiperistalsis Activity
The crude extract (Hh.Cr) showed a significant antiperistalsis response in mice with less distance traveled by charcoal meal as compared to control (33 ± 2.3%).The group was treated with 400 mg/kg of Hh.Cr and CCh (3 mg/kg) and peristaltic movements were significantly decreased by 1.2 ± 0.37 and 8.6 ± 1.8, respectively (Figure 7).

Antiperistalsis Activity
The crude extract (Hh.Cr) showed a significant antiperistalsis response in mice with less distance traveled by charcoal meal as compared to control (33 ± 2.3%).The group was treated with 400 mg/kg of Hh.Cr and CCh (3 mg/kg) and peristaltic movements were significantly decreased by 1.2 ± 0.37 and 8.6 ± 1.8, respectively (Figure 7).

In Silico Studies
The docking calculations are beneficial to predict ligand pose within the binding site of the target protein.The involvement of physical energies terms (i.e., solvation energy) with suitable force field make docking calculation of compounds more acceptable with accuracy (Table 2) [12][13][14].
Molecular docking for cyclooxygenase-2 enzyme: The selected compounds were studied against cyclooxygenase-2 enzyme (COX-2, PDB ID:5IKQ) for anti-inflammatory activity (Table 2, Figure 8).Arctiin was predicted with the lowest binding energy (∆G bind : −41.01 kcal/mol) among the selected compounds.As mentioned earlier, Van der Waals (∆G vdW ) and lipophilic interactions (∆G Lipo ) are significant contributors to the ligand binding energy.It was observed that the binding energies value of ∆G vdW was −36.96 kcal/mol, and ∆G Lipo was −23.77 kcal/mol, whereas hydrogen bond (∆G Hbond ) energy contribution was −2.56 kcal/mol.It also formed hydrophobic interactions π-π T shaped interaction with Tyr11.Arctigenin second to arctiin in docking score was found with potential hydrophobic interactions within hydrophobic clefts of COX-2.The ligand binding energy of arctigenin (∆G bind : −27.91 kcal/mol) was driven mainly by these hydrophobic interaction energies; ∆ G vdW (−35.44 kcal/mol) and ∆G Lipo (−31.09kcal/mol) and formed hydrophobic π-σ interaction with Val117.The ranking orders of ligands with COX-2 are given below: arctiin > arctigenin > cynaropicrin > catechin.

Discussion
Himalaiella heteromalla has a potential pharmacological role in the management of various diseases.This research was employed to investigate its pharmacological characteristics.The presence of alkaloids, glycosides, triterpenoids, flavonoids, saponins, sesquiterpene, which play a vital role in the pharmacological potential of Himalaiella heteromalla.The HPLC results indicate the presence of gallic acid, catechin, HB acid, and vanillin acid.Gallic acid (3,4,5trihydroxybenzoic acid), a natural polyphenol product, has

Discussion
Himalaiella heteromalla has a potential pharmacological role in the management of various diseases.This research was employed to investigate its pharmacological characteristics.The presence of alkaloids, glycosides, triterpenoids, flavonoids, saponins, sesquiterpene, which play a vital role in the pharmacological potential of Himalaiella heteromalla.The HPLC results indicate the presence of gallic acid, catechin, HB acid, and vanillin acid.Gallic acid (3,4,5trihydroxybenzoic acid), a natural polyphenol product, has anti-oxidant, anti-inflammatory, antimicrobial, and radical scavenging activities.Gallic acid is used as a spasmolytic effect on smooth muscle isolated jejunum tissues and trachea by calcium chan-nel blocking activity [15].Gallic acid is used as an antispasmodic in diarrhea [16].Gallic acid possesses an anti-inflammatory effect [17].Catechin abundant flavonoid present in plants, it reported for several gastrointestinal, respiratory, and inflammatory disorders [18][19][20].Vanilla acid and HB acid are polyphenolics used for gastrointestinal, respiratory, and cardiovascular disorders by spasmolytic effects on isolated tissues of the jejunum, trachea, and aorta [20].The water content in H. heteromalla play a vital role in the biological activities, so it is more important to measure the water content in H. heteromalla therefore, infra red radiation can be used to measure water content determination [21].
Himalaiella heteromalla crude extract (Hh.Cr) was studied on isolated jejunum preparations to elaborate the mechanism of H. heteromalla in gastrointestinal diseases.It is reported that jejunum preparations have rhythmic contractions due to the influx of calcium ions and potassium ions through their respective ions channels.H. heteromalla crude extract and its fraction ethyl acetate exerted spasmolytic response in dose concentration when exposed to spontaneous contraction of jejunum preparations [22].Thus, H. heteromalla crude extract and its fraction ethyl acetate showed the antispasmodic response by suppressing rhythmic contractions in jejunum preparations.These results indicate that H. heteromalla crude extract and its fraction ethyl acetate decrease or blockade the cytoplasmic free Ca ++ ions through the blockade of voltage-dependent calcium ion channels.As a result, activation of calmodulin and other contractile proteins, i.e., actin and myosin, does not occur [23].H. heteromalla crude extract and its fraction ethyl acetate and aqueous were exposed to K + (80 mM)-induced contractions on jejunum preparations, H. heteromalla crude extract and its fraction ethyl acetate relaxed the K + (80 mM)-induced contractions in dose concentration manner in a tissue organ bath.It was previously reported that K + (80 mM) induces contractions to cause cell depolarization by the influx of calcium ions into the cell through the voltage-gated L-type calcium ion channel [24].Similar to verapamil, any substance inhibited K + (80 mM)-induced contractions were considered calcium channel blockers (CCB).Thus, H. heteromalla crude extract and its fraction ethyl acetate blockade the calcium influx into the cell by alternating or binding with voltage-dependent calcium channels.Furthermore, calcium concentration-response curves (CRCs) were constructed on pretreated Hh.Cr jejunum preparations to confirm the calcium channel blockade activity of Hh.Cr in a tissue organ bath.The results showed that partial blockade with the rightward parallels dose-response curves at low doses while completely blocking the dose-response curves at 0.3 mg/m.Thus, Himalaiella heteromalla exhibited a strong calcium antagonistic effect [25].
To evaluate another possible mechanism of H. heteromalla crude extract on the gastrointestinal tract, Hh.Cr was studied in antiperistalsis and antidiarrheal in vivo models.H. heteromalla crude extract showed the antispasmodic response by inhibiting the traveling of charcoal meal in antiperistalsis activity.H. heteromalla crude extract also inhibited diarrheal response in castor oil-induced diarrhea.It decreased the wet feces by inhibiting the electrolyte and water imbalance that may cause diarrhea in rats [26].
H. heteromalla crude extract and its fractions ethyl acetate and aqueous were tested for possible bronchodilator activity against CCh (1 µM) and K + (80 mM)-induced contractions on tracheal preparations.The results showed that H. heteromalla crude extract and its fractions ethyl acetate exhibited relaxant response against CCh (1 µM) and K + (80 mM)-induced contractions, but a partial relaxant effect was observed by the aqueous fraction.However, EC 50 of H. heteromalla crude extract and its fractions ethyl acetate against K + (80 mM)induced contractions that were more minor than CCh-induced contractions, similar to that of verapamil.CCh is a cholinergic agonist which causes smooth muscle contraction through activation of muscarinic receptors.Hence, H. heteromalla crude extract and its fractions ethyl acetate showed bronchodilator response was found due to Ca ++ ion channel and muscarinic receptor blockade.Nowadays, Ca ++ channel blockers and muscarinic antagonists are used to treat the relief from respiratory diseases such as asthma [27,28].
H. heteromalla crude extract and its fractions ethyl acetate and aqueous were tested for possible vasorelaxant activity against PE (1 µM) and K + (80 mM)-induced contractions on aortic preparations.The results showed that H. heteromalla crude extract and its fractions ethyl acetate exhibited a relaxant response against PE (1 µM) and K + (80 mM)-induced contractions, similar to verapamil.H. heteromalla aqueous fraction partially relaxed the PE and K + (80 mM) induced contractions.Relaxation of the PE (PE) and K + (80 mM)induced contractions indicates a blockade of intracellular Ca ++ influx by blocking Ca ++ channels.Ca ++ channel blockers are essential drugs used clinically to manage angina and hypertension [29,30].
H. heteromalla crude extract and its fractions ethyl acetate and aqueous were tested on paired atrium for possible effects on force and rate of atrial contractions.H. heteromalla crude extract and its fractions ethyl acetate and aqueous showed cardio suppressant response via blocking calcium channels, hence Hh.Cr and its fractions were found with adverse inotropic and chronotropic effects on the paired atrium [31].
Himalaiella heteromalla crude extract was tested for anti-inflammatory activity.It was found that Hh.Cr blocked the release of inflammatory mediators in rat paw edema and other models.It is reported that carrageenan acetic acid and formalin release inflammatory mediators such as bradykinin, histamine, TNF, IL-1b, IL-6, PEG2, and TNF were blocked by crude extract of crude extract Himalaiella heteromalla.The reduction in inflammatory mediators by carrageenan, inducing the rat's paw edema model to show that Hh.Cr inhibits factors that cause inflammation and swelling.On the other side, pain sensation is a significant indicator in the inflammation process, which Hh blocked Cr, so that it exhibited analgesic activity.This anti-inflammatory result was compared with standard drug analgesic aspirin, reducing all models' inflammation and pain sensations.The comparative results in between aspirin and Hh.Cr showed that Hh.Cr exhibited thde same potential as aspirin to reduce the pain and inflammation via blockading inflammatory mediators [32].
Molecular docking is a helpful tool to predict the possible mechanism of actions of the selected compounds of various pharmacological studies-the present study correlated and defined antispasmodic and anti-inflammatory activities of Himalaiella heteromalla.The five compounds of H. heteromalla were studied for cyclooxygenase 2, lipoxygenase 5, and muscarinic M3 receptor.The docking calculations of these compounds indicate the presence of antispasmodic and anti-inflammatory activities, which were previously proven in experimental studies.Arctiin and arctigenin were more potent compounds responsible for these activities.These results conclude that these compounds interact with cyclooxygenase 2, lipoxygenase 5, and muscarinic M3 receptor to exert the activity.As mentioned earlier, Gao et al. [4] reported that arctigenin and arctiin have anti-inflammatory and vasodilation properties and help treat acute lung injury, local edema, brain trauma, and colitis.These studies support the potent results of arctigenin and arctiin in silico studies against major inflammatory proteins COX-2 and LOX-5.

Extract Preparation
Himalaiella heteromalla (D.Don) Raab-Straube was collected from hilly areas of Islamabad and identified by Dr. Zafarullah Zafar, taxonomist, Institute of Pure and Applied Biology, and submitted with voucher no: http://www.theplantlist.org/tpl1.1/record/gcc-138921dated 18 June 2018.Plant material was ground through a herbal grinder for coarse powder, then powder (1 kg) was macerated in methanol aqueous (70:30) for maceration in an amber color glass bottle for three days at room temperature and periodically shaken 3-4 times a day.The solvent was filtered to remove plant debris with muslin cloth and Whattman-1 filter paper.This procedure was replicated thrice, and the filtrate obtained by all steps was combined and processed in a rotary evaporator (BUCHI) under reduced pressure at 36 ± 2 • C to obtain a brownish colored semi-solid (Hh.Cr) and stored at −20 • C in an airtight jar with a percentage yield of 12%.The Hh.Cr (20 g) was subjected to solventsolvent extraction with ethyl acetate and distilled water to produce an ethyl-acetate fraction (Hh.Ea) and aqueous fraction (Hh.Aq) with approximately 5.5% and 40% yield, respectively.
H. heteromalla crude extract (Hh.Cr) and its fractions were moderately soluble in aqueous.All dilutions were prepared fresh on the day of the experiment.

Animal Housing
Both sexes of albino mice (weight: 20-30 g), rats (weight: 150-200 g), and rabbits (weight: 1-1.8 kg) were used in this study and kept under controlled housing conditions with a temperature of 23 ± 3 • C in the animal house of the Faculty of Pharmacy, Bahauddin Zakariya University, Multan.Before the experiment, animals were deprived of food overnight but had free access to water.For in vitro experimentation, rabbits were sacrificed following a blow, while mice and rats were killed by cervical dislocation.All the experimentations were performed under rules specified by the Institute of Laboratory Animal Resources, Commission on Life Sciences (NRC, 1996) endorsed by the Ethical Committee of Bahauddin Zakariya University, Multan.

HPLC Separation of Phenolic Acids and Flavonoids
The phenolic acids and flavonoids components in Himalaiella heteromalla were quantified by developing a binary gradient solvent system to run in Chromera HPLC system (Perkin Elmer, Houston, TX, USA) consisting of Felexer Binary Liquid chromatography (LC) pump coupled with UV/Vis LC Detector (Shelton, CT, USA) which was operated with the help of a software.HPLC system consisted of a C-18 column (250 × 4.6 mm internal diameter) with a thickness of 5 µM film.The mobile phase consisted of solvent A (methanol (30): acetonitrile (70)) and solvent B (0.5% glacial acetic acid in double-distilled water), mobile phase run at flow rate 0.08 mL/min, and data was recorded at 275 nm of UV spectra.The peaks and retention times of phenolic acids and flavonoids of H. heteromalla were matched with external standards to quantify the components [12].The resolution and separation factor was used to determine HPLC separation efficiency.

In Vitro Experiments
The physiological response of tissues was recorded with isotonic and force-displacement isometric transducers amplified with acquisition system Power Lab (AD Instruments, Bella Vista, NSW, Australia) coupled with a computer having Lab chart Pro.The effect was taken as percent change on the part of test substance recorded instantly preceding a dose of test substance [22].

Isolated Rabbit Jejunum Preparation
The jejunum was dissected from a rabbit; the adhesive fatty tissues were carefully removed, and then ~2 cm long piece of jejunum was prepared.This tissue was hung in an organ bath containing Tyrode's solution with a continuous supply of carbogen (95% O 2 + 5% CO 2 ) at 37 • C and equilibrated for 30 min.Acetylcholine (1 µM) was added to spontaneous rhythmic contractions of jejunum for control response and washed it.The Hh.Cr was added cumulatively for antispasmodic effect.The spontaneous contractions jejunum preparation was exposed to K + (80 mM) induced contraction for estimation of CCB activity [33].
The extract was exposed to calcium concentration response curves (CRCs) for further confirmation.The jejunum preparation was stabilized in Tyrode's solution, subsequently replaced with calcium-free Tyrode's solution with EDTA (0.1 mM) to remove calcium from tissue.Afterward, with an incubation duration of 40 min, two superimposable control calcium CRCs were constructed in an organ bath, then tissue was incubated with the plant exact for one h, and calcium CRCs were obtained and compared to control.The calcium CRCs were recorded in the presence of different concentrations of plant extract.

Isolated Rabbit Tracheal Preparations
The trachea was dissected from a rabbit for bronchodilator activity, and the 2 mm tracheal ring tissue was prepared.A longitudinal incision was made opposite the smooth muscle layer to form a strip.This tracheal preparation was hung in an organ bath containing Krebs's solution with a continuous supply of carbogen at 37 • C. Preload tension (1 g) was applied and allowed to equilibrate for 60 min prior to the dose of any drug.The tracheal preparation was exposed to CCh (1 µM), and K + (80 mM) induced contraction for bronchodilator activity in a cumulative manner.

Isolated Rabbit Paired Atria Preparations:
The heart was dissected from a rabbit for cardiac activity, and the ventricles were carefully removed to isolate paired atria.This atrium preparation was hung in an organ bath containing Krebs's solution with a continuous supply of carbogen at 34 • C.Then, 1 g preload tension was applied and allowed to equilibrate for 30 min prior to the dose of any drug.The isolated atrium preparation was exposed for possible cardiac effects in a cumulative fashion, and changes in rate and force of contractions were observed.

Isolated Rabbit Aorta Preparations
For vasorelaxant activity, the thoracic aorta was dissected from a rabbit, carefully removed the adhesive fatty tissues, and prepared 2-3 mm aortic rings.This aortic preparation was hung in an organ bath containing Krebs's solution with a continuous supply of carbogen.Preload tension (2 g) was applied and allowed to equilibrate for 60 min prior to the dose of any drug.The isolated aortic preparation was exposed to H. heteromalla for possible vasorelaxant effects in a cumulative manner.Further to define the possible mechanism, H. heteromalla was challenged to PE (1 µM), and K + (80 mM) induced contraction for the possible activity of H. heteromalla in a cumulative manner.

Antiperistalsis Activity
Antiperistalsis activity was performed according to the method prescribed by Wahid et al. [12].Mice (25) of either sex were divided into 5 groups, i.e., control (0.9% normal saline), standard drug (CCh10 mg/kg), and H. heteromalla doses (100, 200, and 400 mg/kg).After 15 min of administering the test or standard material orally, each animal received 0.3 mL of the charcoal meal (10% gum acacia, 20 starch, and 10% vegetable charcoal) in distilled water.Thirty minutes later, mice were killed, and the abdomen was incised to excise the whole small intestine.The distance from the pylorus region was measured to the front of the charcoal meal.

Antidiarrheal Activity
The antidiarrheal activity was performed according to the method prescribed by Wahid et al. [12] with modifications.Mice (20) of either sex were divided into five groups, i.e., negative control (0.9% normal saline), standard drug (loperamide 10 mg/kg), and H. heteromalla doses (100, 200, and 400 mg/kg).After 30 min of dose administration (p.o.), animals received the castor oil (10 mL/kg p.o) and were observed for six hours in cages with a white paper surface with adsorbent properties.The percent inhibition of wet fecal was calculated.

Carrageenan-Induced Rat's Hind Paw Edema Method
The anti-inflammatory activity was performed [32,34] on 25 rats of either sex divided into 5 groups, i.e., control (0.9% normal saline) standard drug (aspirin 10 mg/kg), and H. heteromalla doses (100, 200, and 400 mg/kg).After 30 min of dose administration (p.o), edema was induced by injecting 1% carrageenan in the right hind paw's sub-planter region and measuring the edema size at up to 4 h through a plethysmometer.The percentage of edema inhibition was calculated.

In Silico Studies
In silico studies were performed according to the method previously reported by Wahid et al. [12] and Sirous et al. [13].
Ligand Preparation: The 2D structures of HPLC quantified phytocompounds were retrieved from PubChem (https://pubchem.ncbi.nlm.nih.govaccessed date 20 March 2019) and treated in the LigPrep module of Maestro (Schrodinger suite 2015) to ionization, minimization, and optimization of ligands.The Epik tool of this module was used to generate the ionization state of ligands at cellular pH (7.4 ± 0.5) and applied the OPLS3e force field through the module for minimization and optimization of ligands that produce the lowest energy conformer of ligands.
Protein Preparation: For molecular docking, the highest resolution X-ray structures of proteins were downloaded from The Protein Databank (RCSB PDB) (https://www.rcsb.orgaccessed date 20 March 2019) and subjected to Protein preparation wizard of Maestro (Schrodinger suite 2015).This module processed the protein by adding hydrogen atoms to protein structure, removal of solvents (water) molecules, assigning bond orders, creating disulfide bonds, filling missing side chains and loops, and generating protonation state using Epik tool of protein structures for ligands at the cellular level pH (7.4 ± 0.5).After processing protein structures, these structures were optimized using PROPKA under pH 7.0, and the OPLS3e force field was utilized to perform restrained minimization for energy minimization and geometry optimization of protein structure.
Molecular Docking and Receptor grid generation: The active sites of protein structures for molecular docking were defined in the Receptor Grid Generation module of Maestro (Schrodinger suite 2015).A cubic grid box of each protein was defined with the help of a literature survey and with a selection of previously bonded ligands of proteins.The length of the grid box was adjusted to the length of 16 Å.The potential of nonpolar parts of the receptor was decreased to scaling factor 1.0 Å on Van der Waals radius of nonpolar atoms of protein having partial atomic charge cut-off 0.25 Å.
For molecular docking, the prepared ligands and protein structures were subjected to extra precision (XP) mode of Ligand Docking (Glide) module of Maestro (Schrodinger suite 2015) using pre-generated grid file for receptor.Additionally, 0.80 Å scaling factor was adjusted for Van der Waals radii with a partial charge cut-off of 0.15 Å.The docking results were subjected to the Prime MM-GBSA module to calculate the binding energies of ligands with protein structure using the VSGB solvation model with OPLS3e force field.
Inhibition Constant (K i ): The inhibition constant was determined from the binding free energy of ligand previously generated from Prime MM-GBSA, according to the following equation [12]: ∆G = −RT(lnK i ) or K i = e (−∆G/RT) where ∆G is binding free energy of ligand, R is gas constant (cal•mol −1 •K −1 ), and T is room temperature (298 Kelvin).

Statistical Analysis
The data were expressed as the mean ± standard error of the mean (S.E.M.) and median effective concentration (EC 50 ) with a 95% confidence interval (CI).One-way and two-way ANOVA tests were applied for in vivo experiments.All graphs and data were analyzed with the help of Graph pad prism software (San Diego, CA, USA).

Conclusions
Himalaiella heteromalla exhibited a more spasmolytic effect in ethyl acetate fraction and caused complete relaxation on isolated jejunum, trachea, aorta, and paired atria, supported with in silico studies.H. heteromalla proved various disease management-related activities.Further studies could be taken on Himalaiella heteromalla for drug discovery for the welfare of human beings.

Figure 3 .
Figure 3.Effect of crude extract (Hh.Cr) Ethyl acetate fraction (Ea.Hh) and aqueous fraction (Ea.Aq) on (A) spontaneous contraction and (B) K + (80 mM)-induce Contraction on rabbit jejunum preparations.(C) Effect of verapamil on spontaneous and K + induced contraction on rabbit jejunum preparations.Dose-response curves of Ca ++ in the presence and absence of (D) Hh.Cr (E) verapamil in the isolated rabbit jejunum preparations.Values are expressed as mean ± SEM.

Figure 3 .
Figure 3.Effect of crude extract (Hh.Cr) Ethyl acetate fraction (Ea.Hh) and aqueous fraction (Ea.Aq) on (A) spontaneous contraction and (B) K + (80 mM)-induce Contraction on rabbit jejunum preparations.(C) Effect of verapamil on spontaneous and K + induced contraction on rabbit jejunum preparations.Dose-response curves of Ca ++ in the presence and absence of (D) Hh.Cr (E) verapamil in the isolated rabbit jejunum preparations.Values are expressed as mean ± SEM.

Figure 6 .
Figure 6.(A) Spontaneous contraction.Effect of (B) crude extract (Hh.Cr), (C) Ethyl acetate fraction (Ea.Hh), and (D) Aqueous fraction (Ea.Aq) on spontaneous contraction rabbit paired atrium preparations.(E) Effect of verapamil on spontaneous contraction rabbit paired atrium preparations.Effect of crude extract (Hh.Cr) Ethyl acetate fraction (Ea.Hh) and Aqueous fraction (Ea.Aq) on (F) K + (80mM)-induced contraction.(G) force of contraction.(H) Effect of verapamil on the force of contraction and heart rate on rabbit atrium preparations.Values are expressed as mean ± SEM.

Figure 6 .
Figure 6.(A) Spontaneous contraction.Effect of (B) crude extract (Hh.Cr), (C) Ethyl acetate fraction (Ea.Hh), and (D) Aqueous fraction (Ea.Aq) on spontaneous contraction rabbit paired atrium preparations.(E) Effect of verapamil on spontaneous contraction rabbit paired atrium preparations.Effect of crude extract (Hh.Cr) Ethyl acetate fraction (Ea.Hh) and Aqueous fraction (Ea.Aq) on (F) K + (80mM)-induced contraction.(G) force of contraction.(H) Effect of verapamil on the force of contraction and heart rate on rabbit atrium preparations.Values are expressed as mean ± SEM.

Table 1 .
Phenolic and flavonoid compounds of Hh.Cr.

Table 1 .
Phenolic and flavonoid compounds of Hh.Cr.

∆G Coulomb ∆G Covalent ∆G Hbond ∆G Lipophilic ∆G Solv GB ∆G vdW Residue-Ligand Interactions with Distance (Å)
∆G Binding : Binding free energy, Log Ki: Logarithmic of Inhibition Constant (K i ), ∆G Coulomb : Coulomb binding energy, ∆G Covalent : Covalent binding energy ∆G Hbond : Hydrogen bonding energy, ∆G Lipophilic : Lipophilic binding energy, ∆G Solv GB : Generalized born electrostatic solvation energy ∆G vdW : Van der Waals forces energy, and C-H Bond: Carbon-Hydrogen Bond.These all contribute to Binding free energy (∆G Binding ).