Phytochemical Analysis of the Fruits of Sea Buckthorn (Hippophae rhamnoides): Identification of Organic Acid Derivatives

Hippophae rhamnoides L. (Elaeagnaceae), commonly known as “Sea buckthorn” and “Vitamin tree”, is a spiny deciduous shrub whose fruit is known for its nutritional composition, such as vitamin C, and is consumed as a dietary supplement worldwide. As part of our ongoing efforts to identify structurally new and bioactive constituents from natural resources, the phytochemical investigation of the extract of H. rhamnoides fruits led to the isolation of one malate derivative (1), five citrate derivatives (2–6), and one quinate derivative (7). The structures of the isolated compounds were elucidated by analysis of 1D and 2D nuclear magnetic resonance (NMR) spectroscopic data and high-resolution electrospray ionization (HR-ESI) liquid chromatography–mass spectrometry (LC/MS) data. Three of the citrate derivatives were identified as new compounds: (S)-1-butyl-5-methyl citrate (3), (S)-1-butyl-1′-methyl citrate (4), and (S)-1-methyl-1′-butyl citrate (6), which turned out to be isolation artifacts. The absolute configurations of the new compounds were established by quantum chemical electronic circular dichroism (ECD) calculation, which is an informative tool for verifying the absolute configuration of organic acid derivatives. The isolated compounds 1–7 were evaluated for their stimulatory effects on osteogenesis. Compounds 1, 3, 4, 6, and 7 stimulated osteogenic differentiation up to 1.4 fold, compared to the negative control. These findings provide experimental evidence that active compounds 1, 3, 4, 6, and 7 induce the osteogenesis of mesenchymal stem cells and activate bone formation.


Introduction
Hippophae rhamnoides L. (Elaeagnaceae), known as "Sea buckthorn" and "Vitamin tree", is a spiny deciduous shrub. It has historically been used for medicinal purposes worldwide, especially in Central and Southeastern Asia. In China, its pharmacological use was revealed more than a thousand years ago through the records of Sibu Yidian from the Tang Dynasty and Jing Zhu Ben Cao from the Qing dynasty [1]. Sea buckthorn oil was listed in the Russian Pharmacopeia in the 1950s and used as an anti-inflammatory and wound healing agent in Russia [2]. Its bark and fruits have also been used as Tibetan folk medicine to treat pulmonary disorders, cough, fever, and tumors [3]. For the past 50 years, several medicinal preparations based on H. rhamnoides have been clinically used to treat radiation damage, oral inflammation, burns, and gastric ulcers, and more than 300 preparations have been reported in the literature [4].
H. rhamnoides bears yellowish or orange berries, which are commonly found as a powdered drink mix in markets as a functional food and are used to make fruit sauce and wine [5]. Vitamin C is a major nutritional component of H. rhamnoides fruits [6], the level of which exceeds that of lemons and oranges. Previous pharmacological studies of H. rhamnoides have reported that its extracts exhibited therapeutic properties, including anti-platelet effects via the inhibitory mechanism of thrombin-activated platelets and antimicrobial effects against S. aureus and C. albicans through the inhibition of adhesion and biofilm formation [7,8]. In addition, previous phytochemical investigations on H. rhamnoides have demonstrated the presence of diverse types of bioactive substances, including vitamins (A, E, K, riboflavin, and folic acid), carotenoids, phytosterols, minerals, organic acids, polyunsaturated fatty acids, and amino acids [9,10]. Because of the nutritional importance of H. rhamnoides fruits, many research groups have attempted to investigate the phytochemical constituents of these fruits, in which flavonoids, proanthocyanidins, alkaloids, and phenolic acids have been identified [11,12]. Nevertheless, previous findings suggested that only few studies have been carried out on the chemical constituents of H. rhamnoides fruits, despite their health benefits.
In this context, as a part of continuous research programs to discover structurally new bioactive compounds from diverse natural sources [13][14][15][16][17][18][19], our group has focused on the potential bioactive composition of H. rhamnoides fruits. In our recent study of H. rhamnoides fruits, we identified six chemical compounds, including one citric acid derivative, two flavonoids, one phenolic compound, and two megastigmane derivatives, which were evaluated for their anti-inflammatory effects by determining LPS-induced NO production in RAW 264.7 cells [20]. As a result, we found that a citric acid derivative, 1,5-dimethyl citrate, effectively inhibited LPS-induced NO production and simultaneously showed antiinflammatory effects by inhibiting the expression of pro-inflammatory mediators, iNOS and COX-2, and the activity of pro-inflammatory cytokines, IL-6 and TNF-α [20]. Our recent findings provide experimental evidence that the organic acid derivative present in H. rhamnoides fruits could function as a promising bioactive substance. In the present study, further phytochemical investigation of the extract of H. rhamnoides fruits was performed to identify potential bioactive organic acid constituents based on recent preliminary findings. Phytochemical analysis of the BuOH-soluble fraction derived from the extract led to the isolation of seven organic acid derivatives: one malate derivative (1), five citrate derivatives (2)(3)(4)(5)(6), three of which were identified as new compounds, and one quinate derivative (7). The structures of the isolated organic acid derivatives (1)(2)(3)(4)(5)(6)(7) were determined by analysis of 1D and 2D nuclear magnetic resonance (NMR) spectroscopic data and high-resolution electrospray ionization (HR-ESI) liquid chromatography-mass spectrometry (LC/MS). In addition, the absolute configurations of the new compounds were established using quantum chemical electronic circular dichroism (ECD) calculations. Herein, we describe the isolation and structural elucidation of the organic acid derivatives 1-7 and the evaluation of their biological activities for stimulatory effects on osteogenesis.

Isolation of Compounds
The aqueous extract powder of H. rhamnoides fruits was suspended for solvent partitioning in water and then fractionated with four organic solvents, namely, hexane, dichloromethane, ethyl acetate, and n-butanol. LC/MS-based analysis combined with our in-house built UV library, and TLC analysis of the solvent-partitioned fractions suggested that the BuOH-soluble fraction contained the majority of the organic acid derivatives. Phytochemical analysis of the BuOH-soluble fraction was performed using repeated column chromatography with silica gel 60, RP-C 18 silica gel, Diaion HP-20, Sephadex LH-20, and high-performance liquid chromatography (HPLC). The final semi-preparative HPLC separation afforded seven organic acid derivatives (1-7) (Figure 1). that the BuOH-soluble fraction contained the majority of the organic acid derivatives. Phytochemical analysis of the BuOH-soluble fraction was performed using repeated column chromatography with silica gel 60, RP-C18 silica gel, Diaion HP-20, Sephadex LH-20, and high-performance liquid chromatography (HPLC). The final semi-preparative HPLC separation afforded seven organic acid derivatives (1-7) (Figure 1).

Elucidation of Compound Structures
Compound 3 was isolated as a yellowish gum. The molecular formula was determined to be C 11 Figure S1). The 1 H NMR spectrum ( Figure S2) of 3 (Table 1) showed the presence of characteristic signals of an oxygenated methylene group at δ H 4.08 (2H, t, J = 6.5 Hz), a methoxy group at δ H 3.66 (3H, s), two pairs of relatively deshielded methylene groups overlapped at δ H 2.84 and 2.92, another two pairs of methylene groups at δ H 1.61 (2H, m) and 1.40 (2H, m), and a terminal methyl group at δ H 0.94 (3H, t, J = 7.5 Hz). The 13 C NMR data of 3 (Table 1) assigned with the assistance of the HSQC experiment ( Figure S4) confirmed the 11 carbon signals, which corresponded to three carboxyl carbons (δ C 170.3, 170.5, and 175.3), two oxygenated carbons (δ C 64.2 and 72.9), four sets of methylene groups (δ C 18.6, 30.2, 42.7, and 42.7), one methoxy-oriented carbon (δ C 50.7), and one methyl carbon (δ C 12.5). Based on this evidence, it was predicted that compound 3 was a citric acid derivative, since the characteristic NMR data of 3 was similar to that of the citric acid derivatives [21,22]. The gross structure of 3 was determined by 2D NMR experiments ( 1 H-1 H COSY; Figure S3 and HMBC; Figure S5). The HMBC correlations of H 2 -2/C-1, H 2 -2/C-1 , H 2 -2/C-4, H 2 -4/C-1 , H 2 -4/C-2, and H 2 -4/C-5 verified the citric acid moiety. Furthermore, the HMBC correlation of 5-OCH 3 with C-5 confirmed that the methoxy group was attached at C-5 of the citric acid moiety, and that of H 2 -1" with C-1, along with 1 H-1 H cross-peaks between H 2 -1"/ H 2 -2"/ H 2 -3"/ H 3 -4", revealed that a butyl group was attached at the other end of C-1 ( Figure 2). Thus, the planar structure of 3 was established, as shown in Figure 2. Finally, the absolute configuration at C-3 was assigned using quantum chemical ECD calculations [23][24][25]. Two possibilities with absolute configurations of 3R and 3S were calculated. The weighted calculated ECD spectrum (green line) of 3S (3S) agreed well (although not identical) with the experimental curve of 3, compared to that (red line) of 3R (3R) (Figure 3), thus determining the absolute configuration of C-3 as S. The chemical structure of 3, including its absolute configuration, was elucidated as (S)-1-butyl-5-methyl citrate.
Compound 4 was obtained as a white amorphous powder. The molecular formula was determined to be the same as that of compound 3, C 11 H 18 O 7 , from HRESIMS data ( Figure S6), which showed a protonated molecular ion peak [M + H] + at m/z 263.1119 (calculated for C 11 H 19 O 7 , 263.1131). The 1 H ( Figure S7) and 13 C NMR data ( Figure S9) of 4 ( Table 1) were also almost identical to those of compound 3, except for apparent differences in the chemical shifts responsible for the citric acid moiety-the 1 H chemical shift of the methoxy group (δ H 3.66 in 3, δ H 3.75 in 4). Detailed analysis of the 2D NMR spectra ( 1 H-1 H COSY; Figure S8 and HMBC; Figure S10) assigned the gross planar structure of 4 ( Figure 2), where the key HMBC correlation of 1 -OCH 3 with C-1 was observed, indicating that the methoxy group was attached at C-1 in compound 4 ( Figure 2). Finally, to confirm the absolute configuration of C-3 in 4, the computationally calculated ECD data of the two enantiomers, 3R-and 3S-isomers, were compared with the experimental ECD data of 4 ( Figure 4). The ECD data of 4 showed a good fit with the calculated ECD spectrum (green line) of 4S (3S) (Figure 4). This finding allowed us to assign the structure of 4 as (S)-1-butyl-1 -methyl citrate.

Elucidation of Compound Structures
Compound 3 was isolated as a yellowish gum. The molecular formula was determined to be C11H18O7 from the protonated molecular ion peak [M + H] + at m/z 263.1122 (calculated for C11H19O7, 263.1131) in the positive-ion mode of HRESIMS ( Figure S1). The 1 H NMR spectrum ( Figure S2) of 3 (Table 1) showed the presence of characteristic signals of an oxygenated methylene group at H 4.08 (2H, t, J = 6.5 Hz), a methoxy group at H 3.66 (3H, s), two pairs of relatively deshielded methylene groups overlapped at H 2.84 and 2.92, another two pairs of methylene groups at H 1.61 (2H, m) and 1.40 (2H, m), and a terminal methyl group at H 0.94 (3H, t, J = 7.5 Hz). The 13 C NMR data of 3 (Table 1) assigned with the assistance of the HSQC experiment ( Figure S4) confirmed the 11 carbon signals, which corresponded to three carboxyl carbons (δC 170.3, 170.5, and 175.3), two oxygenated carbons (δC 64.2 and 72.9), four sets of methylene groups (δC 18.6, 30.2, 42.7, and 42.7), one methoxy-oriented carbon (δC 50.7), and one methyl carbon (δC 12.5). Based on this evidence, it was predicted that compound 3 was a citric acid derivative, since the characteristic NMR data of 3 was similar to that of the citric acid derivatives [21,22]. The gross structure of 3 was determined by 2D NMR experiments ( 1 H-1 H COSY; Figure S3 and HMBC; Figure S5). The HMBC correlations of H2-2/C-1, H2-2/C-1', H2-2/C-4, H2-4/C-1', H2-4/C-2, and H2-4/C-5 verified the citric acid moiety. Furthermore, the HMBC correlation of 5-OCH3 with C-5 confirmed that the methoxy group was attached at C-5 of the citric acid moiety, and that of H2-1'' with C-1, along with 1 H-1 H cross-peaks between H2-1''/ H2-2''/ H2-3''/ H3-4'', revealed that a butyl group was attached at the other end of C-1 ( Figure 2). Thus, the planar structure of 3 was established, as shown in Figure 2. Finally, the absolute configuration at C-3 was assigned using quantum chemical ECD calculations [23][24][25]. Two possibilities with absolute configurations of 3R and 3S were calculated. The weighted calculated ECD spectrum (green line) of 3S (3S) agreed well (although not identical) with the experimental curve of 3, compared to that (red line) of 3R (3R) (Figure 3), thus determining the absolute configuration of C-3 as S. The chemical structure of 3, including its absolute configuration, was elucidated as (S)-1-butyl-5-methyl citrate.   Compound 4 was obtained as a white amorphous powder. The molecular formula was determined to be the same as that of compound 3, C11H18O7, from HRESIMS data ( Figure S6), which showed a protonated molecular ion peak [M + H] + at m/z 263.1119 (calculated for C11H19O7, 263.1131). The 1 H ( Figure S7) and 13 C NMR data ( Figure S9) of 4 (Table 1) were also almost identical to those of compound 3, except for apparent differences    Figure S11). The 1 H ( Figure S12) and 13 C NMR data ( Figure  S14) (Table 1) of 6 were also quite similar to those of compounds 3 and 4, with the only noticeable differences being that the 13 C chemical shift of C-1 was deshielded to δC 173.9, compared to these two compounds (δC 170.2 in 3, δC 170.0 in 4), and the 1 H chemical shift of H-1'' was deshielded to δH 4.18, compared to 3 and 4 (δH 4.08 in 3, δH 4.07 in 4). This finding suggested the possibility that the butyl group in 6 was attached to the different carboxyl groups of compounds 3 and 4. The gross planar structure of 6 was verified by 2D NMR analysis ( 1 H-1 H COSY; Figure S13 and HMBC; Figure S15) ( Figure 2). The key HMBC correlation between 1-OCH3 and C-1 supported the presence of the methoxy group at C-1, and the location of the butyl group was determined to be C-1' by the key HMBC correlation of H-1'' and C-1' (Figure 2). Finally, the absolute configuration of 6 was assigned using quantum chemical ECD calculations. As shown in Figure 5, the experimental ECD curve of 6 is consistent with the calculated ECD spectrum (green line) of 6S (3S). Therefore, the chemical structure of 6 was characterized as (S)-1-methyl-1'-butyl citrate.    Figure S12) and 13 C NMR data ( Figure S14) ( Table 1) of 6 were also quite similar to those of compounds 3 and 4, with the only noticeable differences being that the 13 C chemical shift of C-1 was deshielded to δ C 173.9, compared to these two compounds (δ C 170.2 in 3, δ C 170.0 in 4), and the 1 H chemical shift of H-1" was deshielded to δ H 4.18, compared to 3 and 4 (δ H 4.08 in 3, δ H 4.07 in 4). This finding suggested the possibility that the butyl group in 6 was attached to the different carboxyl groups of compounds 3 and 4. The gross planar structure of 6 was verified by 2D NMR analysis ( 1 H-1 H COSY; Figure S13 and HMBC; Figure S15) ( Figure 2). The key HMBC correlation between 1-OCH 3 and C-1 supported the presence of the methoxy group at C-1, and the location of the butyl group was determined to be C-1 by the key HMBC correlation of H-1" and C-1 ( Figure 2). Finally, the absolute configuration of 6 was assigned using quantum chemical ECD calculations. As shown in Figure 5, the experimental ECD curve of 6 is consistent with the calculated ECD spectrum (green line) of 6S (3S). Therefore, the chemical structure of 6 was characterized as (S)-1-methyl-1 -butyl citrate.   Figure S12) and 13 C NMR data ( Figure  S14) (Table 1) of 6 were also quite similar to those of compounds 3 and 4, with the only noticeable differences being that the 13 C chemical shift of C-1 was deshielded to δC 173.9, compared to these two compounds (δC 170.2 in 3, δC 170.0 in 4), and the 1 H chemical shift of H-1'' was deshielded to δH 4.18, compared to 3 and 4 (δH 4.08 in 3, δH 4.07 in 4). This finding suggested the possibility that the butyl group in 6 was attached to the different carboxyl groups of compounds 3 and 4. The gross planar structure of 6 was verified by 2D NMR analysis ( 1 H-1 H COSY; Figure S13 and HMBC; Figure S15) ( Figure 2). The key HMBC correlation between 1-OCH3 and C-1 supported the presence of the methoxy group at C-1, and the location of the butyl group was determined to be C-1' by the key HMBC correlation of H-1'' and C-1' (Figure 2). Finally, the absolute configuration of 6 was assigned using quantum chemical ECD calculations. As shown in Figure 5, the experimental ECD curve of 6 is consistent with the calculated ECD spectrum (green line) of 6S (3S). Therefore, the chemical structure of 6 was characterized as (S)-1-methyl-1'-butyl citrate.  The structures of the known compounds ( Figure 1) were determined to be (S)-dimethyl malate (1) [26], (S)-5,1 -dimethyl citrate (2) [21], 1 -butyl-1,5-dimethyl citrate (5) [22], and butyl quinate (7) [27] by spectroscopic methods, including 1 H and 13 C NMR spectra, by comparing their spectroscopic data with those previously reported in the literature and MS data obtained from LC/MS analysis. To the best of our knowledge, compounds 2, 5, and 7 are reported from H. rhamnoides for the first time in this study.
In the meantime, it appeared likely that compounds 3-7, including the new compounds, arose from the addition of a butyl group to the corresponding carboxylic acid during the experimental procedure, because n-BuOH was used as the organic solvent for solvent partitioning. In order to verify whether compounds 3-7 were genuine natural compounds or artifacts, the aqueous extract of H. rhamnoides fruits and fractions derived from the extract were subjected to LC/MS analysis, both alone and co-injected with each compound. As a result, there was no peak with molecular ions corresponding to compounds 3-7 in the crude aqueous extract, whereas all the compounds were detected in the BuOH-soluble fraction, suggesting that the compounds with the butyl group may be artifacts produced by the addition of a butyl group during solvent partition with n-BuOH.

Evaluation of Biological Activities of Compounds 1-7
Mesenchymal stem cells (MSCs) in the bone marrow are pluripotent cells that are known to differentiate into osteocytes as well as adipocytes. Since microenvironmental changes cause alterations in the regulation of gene expression in MSC differentiation, where alterations in the expression of the related genes might disturb the balance between osteoprogenitor and adipocyte progenitor cells in osteoporosis patients [28][29][30], a therapy that is able to adjust the gene expression in MSCs would be promising in the management of postmenopausal osteoporosis. To determine the therapeutic effects of the isolated compounds 1-7 in promoting osteogenesis, all the compounds were examined for their effects on the differentiation of murine MSCs into osteoblasts. The murine mesenchymal stem cell line C3H10T1/2 was treated with 20 µM of the compounds during osteogenesis, and the differentiated cells were stained to indicate alkaline phosphatase (ALP) production, which is considered a distinctive marker of osteoblast differentiation [31]. As a result, compounds 1-7 slightly stimulated differentiation of mesenchymal stem cells into osteocytes ( Figure 6). Although all the compounds did not show the superior reaction of ALP staining as in the oryzativol A-treated one, the positive control group with compounds 1-7 showed more than 1.2 times the bone differentiation-promoting effects compared to the negative control group. In particular, compounds 1, 3, 4, 6, and 7 clearly stimulated the differentiation of osteogenic premature cells up to 1.4 times compared to the negative control group (Figure 6). and butyl quinate (7) [27] by spectroscopic methods, including 1 H and 13 C NMR spectra, by comparing their spectroscopic data with those previously reported in the literature and MS data obtained from LC/MS analysis. To the best of our knowledge, compounds 2, 5, and 7 are reported from H. rhamnoides for the first time in this study.
In the meantime, it appeared likely that compounds 3-7, including the new compounds, arose from the addition of a butyl group to the corresponding carboxylic acid during the experimental procedure, because n-BuOH was used as the organic solvent for solvent partitioning. In order to verify whether compounds 3-7 were genuine natural compounds or artifacts, the aqueous extract of H. rhamnoides fruits and fractions derived from the extract were subjected to LC/MS analysis, both alone and co-injected with each compound. As a result, there was no peak with molecular ions corresponding to compounds 3-7 in the crude aqueous extract, whereas all the compounds were detected in the BuOH-soluble fraction, suggesting that the compounds with the butyl group may be artifacts produced by the addition of a butyl group during solvent partition with n-BuOH.

Evaluation of Biological Activities of Compounds 1-7
Mesenchymal stem cells (MSCs) in the bone marrow are pluripotent cells that are known to differentiate into osteocytes as well as adipocytes. Since microenvironmental changes cause alterations in the regulation of gene expression in MSC differentiation, where alterations in the expression of the related genes might disturb the balance between osteoprogenitor and adipocyte progenitor cells in osteoporosis patients [28][29][30], a therapy that is able to adjust the gene expression in MSCs would be promising in the management of postmenopausal osteoporosis. To determine the therapeutic effects of the isolated compounds 1-7 in promoting osteogenesis, all the compounds were examined for their effects on the differentiation of murine MSCs into osteoblasts. The murine mesenchymal stem cell line C3H10T1/2 was treated with 20 µ M of the compounds during osteogenesis, and the differentiated cells were stained to indicate alkaline phosphatase (ALP) production, which is considered a distinctive marker of osteoblast differentiation [31]. As a result, compounds 1-7 slightly stimulated differentiation of mesenchymal stem cells into osteocytes ( Figure 6). Although all the compounds did not show the superior reaction of ALP staining as in the oryzativol A-treated one, the positive control group with compounds 1-7 showed more than 1.2 times the bone differentiation-promoting effects compared to the negative control group. In particular, compounds 1, 3, 4, 6, and 7 clearly stimulated the differentiation of osteogenic premature cells up to 1.4 times compared to the negative control group ( Figure 6).  Fully-differentiated C3H10T1/2 cells were stained with alkaline phosphatase (ALP) at 9 d after osteogenic differentiation with 20 µM of compounds 1-7. (B) ALP enzyme activity was measured in osteogenically differentiated C3H10T1/2 cells treated with compounds 1-7. The values were calculated relatively by setting the untreated negative control to 1. Oryzativol A (5 µM) was added to the experimental set as a positive control and marked as OryA. * denotes p < 0.05 and *** denotes p < 0.001.

Plant Material
Aqueous extract powder of H. rhamnoides fruits was purchased from Korea Beauty and Healthcare Co., Ltd. in October 2018. The material was authenticated by one of the authors (K. H. K.). A voucher specimen of the material (VT-2018) was deposited in the herbarium of the School of Pharmacy, Sungkyunkwan University, Suwon, Korea.

Computational Analysis
All conformers proposed in this study were acquired through the MacroModel (version 2019-3, Schrödinger LLC) module with mixed torsional/low-mode sampling, implemented with the MMFF94 force field. All searches were initially set in the gas phase, with a 10 kJ/mol energy window limit and a maximum of 10,000 steps to thoroughly explore all potential conformers. The Polak-Ribiere conjugate gradient protocol was established with 10,000 maximum iterations and a 0.001 kJ (mol Å) −1 convergence threshold on the rms gradient to minimize conformers [23][24][25]. Conformers proposed in this study (within 5 kJ/mol found in the MMFF force field) were selected for geometry optimization by Tmolex 4.3.1, with the DFT settings of B3-LYP/6-31+G(d,p). ECD calculations for 3R/3S, 4R/4S, and 6R/6S conformers were performed at identical theoretical levels and basis sets. The calculated ECD spectra were simulated by superimposing each transition, where σ is the width of the band at a height of 1/e. ∆E i and R i are the excitation energies and rotatory strengths for transition i, respectively. In this study, the value of σ was 0.10 eV. The excitation energies and rotational strengths for ECD spectra were calculated based on the Boltzmann populations of the conformers, and ECD visualization was carried out using SigmaPlot 14.0.
To investigate the degree of differentiation into osteocytes, cells were stained for alkaline phosphatase (ALP) after 9 days of osteogenic differentiation. After fixation with 4% formaldehyde solution, cells were exposed to 0.4 mg/mL of nitroblue tetrazolium and 0.2 mg/mL of 5-bromo-4chloro-3 -indolyphosphate in alkaline phosphatase (AP) buffer (100 mM Tris-HCl, pH 9.5; 100 mM NaCl, 5 mM MgCl 2 ) in the dark for 4 h. Next, the reaction was stopped with 5 mM EDTA. To measure APL activity, cell lysates were collected from the differentiated osteogenic cells and treated with p-nitrophenyl phosphate (p-NPP) solution (Alkaline Phosphatase Assay Kit (ab83369; Abcam, Cambridge, MA, USA). Color changes of the samples were measured at 405 nm with a SpectraMax M2 Microplate Spectrophotometer.

Statistical Analysis
Each sample was tested in triplicate, and the test was repeated three times. Data are presented as the mean ± standard deviation (S.D.). Differences between the control and experimental groups were analyzed using a two-tailed unpaired Student's t-test; statistical significance was defined as p < 0.05.

Conclusions
In this study, phytochemical examination of the extracts of H. rhamnoides fruits resulted in the isolation of seven organic acid derivatives: one malate derivative (1), five citrate derivatives (2)(3)(4)(5)(6), and one quinate derivative (7). The structure of the isolates was elucidated by analysis of 1D and 2D NMR data and HR-ESIMS data as well as quantum chemical ECD calculations. Three of the citrate derivatives were identified as new compounds: (S)-1-butyl-5-methyl citrate (3), (S)-1-butyl-1 -methyl citrate (4), and (S)-1methyl-1 -butyl citrate (6), which turned out to be isolation artifacts. To the best of our knowledge, compounds 2, 5, and 7 are reported for the first time from H. rhamnoides in this study. An osteogenesis-promoting activity bioassay in C3H10T1/2 cells demonstrated that compounds 1-7 showed little more than 1.2 times the bone differentiation promoting effect, compared to the negative control group. In particular, compounds 1, 3, 4, 6, and 7 clearly stimulated the differentiation of osteogenic premature cells up to 1.4 times, compared to the negative control group. Although the stimulatory effect of the active compounds on osteogenic differentiation was far behind that of the positive control, oryzativol A, compounds 1, 3, 4, 6, and 7 apparently helped to differentiate the mesenchymal stem cells into osteocytes.

Conflicts of Interest:
The authors declare no conflict of interest.