Evaluation of Insecticidal Effects of Plants Essential Oils Extracted from Basil, Black Seeds and Lavender against Sitophilus oryzae

The risk of using synthetic insecticides to the environment, human health, and the emergence of new genera of pests resistant to that kind of drugs, have led to attention in natural compounds. The present study aimed at evaluating the insecticidal activity of 0.25–6 mg/cm2 of basil (Ocimum basilicum), black seeds (Nigella sativa), and lavender (Lavandula angustifolia) essential oils (EOs) against one of the major stored product pests, Sitophilus oryzae (L.). This was done by assessing mortality and repellent percentage assay in the adult stage, as well as analysing up and down-regulated genes associated with toxicity effect of selected EOs. The three studied EOs showed a toxic effect on S. oryzae; where O. basilicum and L. angustifolia EOs explicated 100% mortality at 6 mg/cm2 after 48 and 24 h, respectively. The highest repellence activity was recorded for O. basilicum EO at 0.75 mg/cm2 with value 82.3% after exposure time 5 h. In the highest dose (6 mg/cm2), the maximum up-regulated expression level of detoxification DEGs genes (CL1294 and CL 8) and cytochrome p45o gene (CYP4Q4) in Lavandula angustifolia EOs exhibited 8.32, 6.08, and 3.75 fold changes, respectively, as compared with 4.76 fold at 10 ppm malathion and 1.02 fold change in acetone control.


Introduction
In 2019, the latest forecast cereal production recorded an increase of 1.2% from 2018, to about 2.685 million tons, according to FAO [1]. Rice is one of the most important crops for almost all of the world especially developing countries [2]. Rice is considered a principal diet for more than two billion people because it has minerals, vitamins, fibers, and carbohydrates [3]. According to storage conditions, rice grains could be attacked by different insects and pests such as Sitophillus oryzoe L., which can be affected in quantity and nutritional quality [4].
In developing countries, loss of cereal foods is considered the major problem due to pest infections through storage [5][6][7]. Many insects, mites, and fungi attack stored cereals colony, and specific life stages were removed. Mixed-sex adults were collected at 3-7 days post eclosion.

Isolation of Essential Oils
In this study, three herbal and medicinal plant species; basil flowers (Ocimum basilicum), seeds of black seeds (Nigella sativa), and lavender flowers (Lavandula angustifolia), were selected according to their ethnomedicinal importance and literature survey and were collected from Botanical Gardens and Ornamental Plants Department, Horticulture Research Institute (HRI) Institute, Agricultural Research Center, Egypt, and the botanical identification was conducted by Prof. Ibrahim Mashaly, Professor of Flora and plant Ecology at Department of Botany, Faculty of Science, Mansoura University, Egypt in July 2019. Plants were shadow dried at room temperature and 50 g were packed and stored at −4 • C. Oils were extracted through steam-distillation utilizing a Clevenger-type apparatus [37]. After a distillation time of 8 h, 50 g of each plant of the dried material yielded nearly 2 mL oil. The distillation was repeated to obtain the required oil quantity for research purposes.

Preliminary Phytochemical Analysis of EOs
Phytochemical characteristics of the essential oils isolated from the selected basil flowers, black seeds, and lavender leaves were illustrated using the following tests:

Screening for Carbohydrate Test
First, 1 mL of EOs of selected plants was added to 1 mL of Benedict's reagent, and the mixture was then heated for 2 min in a boiling water bath. A green solution indicated the presence of reducing sugar.

Screening for Glycosides (Keller Kilianin Test)
Then, 5 mL of isolated essential oils were added with 2 mL of glacial acetic acid, few drops of ferric chloride solution and 1 mL of concentrated sulfuric acid, leading to brown ring formation at the interface, indicating the presence of glycosides.

Screening for Terpenoids (Salkowski Test)
Then, 5 mL of isolated essential oils were taken from the plants, and 2 mL of chloroform and 3 mL of concentrated sulfuric acid were then added. If a reddish-brown layer formed at the junction of the two solutions, the presence of terpenoids was indicated.

Screening for Steroids
Then, 1 mL of extracted essential oils was dissolved in 10 mL chloroform, followed by the addition of an equal volume of sulfuric acid. Red color appeared in the upper and yellow color with green fluorescence developed in the sulfuric acid layer, revealing the presence of steroids.

Screening for Tannins
Then, 2 mL of essential oils extracted from three selected plants were added to a few drops of 1% lead acetate. A yellowish precipitate was developed as a result of tannins presence in the solution.

Insect Mortality
To determine the mortality effect of the studied EOs against Sitophilus oryzae against Sitophilus oryzae, contact effect was evaluated on filter paper discs through treatment of a Whatman No.1, 8 cm diameter, area = 54.4 cm 2 ). The filter paper discs were treated with 0.5 mL of acetonic solutions of EOs, and in the control treatment, the filter paper disc was treated with an equal volume of acetone. The four replicates for each treatment from different EOs solutions were tested corresponding to their doses (2, 4, and 6 mg/cm 2 ) compared with positive control treatment and 10 ppm of the chemical standard malathion. Treated and control filter paper halves were air-dried for 1 h for solvent evaporation. Then, 10 unsexed adult beetles (3-7 days old) were released in the filter paper center, and the lid was then sealed with Parafilm. The experiment was run in the dark at 28 ± 2 • C and 65 ± 5% RH. The dead insects were counted at 3, 6, 12, 24, and 48 h post-application. Insects without response to the gentle touch of a small probe were considered dead [38] and data were corrected using Abbott's formula [39].

Insect Repellence (Filter Paper Disc Bioassay)
A bioassay system was used to evaluate the activity of the three studied EOs using the area preference method [40]. A filter paper disk (Whatman No. 1, 8 cm diameter, area = 54.4 cm 2 ) was divided into 2 halves, one of which was then treated with 0.5 mL of acetonic solutions of EOs, and the control half was treated with an equal volume of acetone. The four replicates from EOs acetone solutions were tested corresponding to the doses of 0.25, 0.5, and 0.75 mg/cm 2 compared with the positive control treatment (malathion, 4 ppm) as chemical control. The treated and control filter paper half were air-dried for about 60 min, allowing solvent evaporation. After that, the paper disc was joined and fixed on the bottom of a Petri dish, and 10 3-7 days old, unsexed adults were then released in the center of both halves. The experiment was maintained at 28 ± 2 • C and 65 ± 5% RH in a dark place. Repellency percentage was recorded at 1, 2, 3, 4, and 5 h depending on the number of insects noticed on both treated and untreated halves. Percentage repellency was calculated by the following equation: where Nc = the number of insects on the untreated half, and Nt = the number of insects on the treated half, after the time exposure.

Chemical Composition of EOs by GC-MS
For determination of the chemical composition of basil, black seeds, and lavender EOs, gas chromatography-mass spectrometry (GC-MS) analysis was conducted using GC-2010 Shimadzu capillary gas chromatography directly coupled to the mass spectrometer system (GC-MS-model QP 2010; (Shimadzu, Kyoto, Japan) DB-c18 column under following conditions: Injector temperature is 250 • C. Oven temperature program: initial temperature 30 • C for 2.0 min, ramp 2.0 • C/min to 250 • C, hold for 5.0 min. MS source temperature is 200 • C, electron energy is 70 eV; the carrier gas was helium at a flow rate 1.4 mL/min; 1 µL of each diluted sample in n-hexane (1:1, v/v) was injected. EI spectra were scanned from 43.00 to 600 m/z Identification of peaks through NIST mass data search libraries and the highest REV and for similarity indicators hits. The components of the sample were identified based on a comparison of their relative indices and mass spectra by computer matching with WILEY and National Institute of Standards and Technology (NIST08) libraries provided with the computer controlling GC-MS system [41]. Total phenolic content in three studied essential oils was determined using the Folin Ciocalteu (FC) method of Su [38] method with slight modifications as prepared at a concentration of 1 mg/mL. A calibration curve was prepared using gallic acid (1-0.05 mg/mL). Then, 1 mL of O. basilicum, N. sativa, and L. angustifolia EOs or gallic acid was added to 0.5 mL of FC reagent and was vortexed well, followed by standing at room temperature for 5 min. Then, 1 mL, 7.5% w/v, of sodium carbonate, was added and maintained for 1 h at room temperature. The absorbance was measured at a wavelength of 760 nm. TPC was expressed as mg gallic acid equivalents (GAE)/g sample.

Estimation of Total Flavonoid Content (TFC)
Total flavonoid content in three studied EOs was determined by the colorimetric method [42]. About 300 µL of three studied EOs were mixed with 30 µL of 16% NaNO 2 . The mixture was then maintained at room temperature for 1 h, and 200 µL of NaOH solution (1 M) was added, followed by 60 µL of 10% AlCl 3 , and 700 µL H 2 O was then added to the mixture. The volume of the mixture was completed up to 1 mL with distilled water. The absorbance of the mixture was measured at 510 nm. The results were expressed as mg of rutin equivalent per gram of fresh weight (mg RE/g FW).

Antioxidant Activity by Free Radical Scavenging Capacity (DPPH)
To assay the free radical scavenging capacity of the investigated EOs on the stable free radical 1, 1-Diphenyl-2-picrylhydrazyl (DPPH) was determined according to [42]. Typically, 100 µL of DPPH (0.004% prepared in methanol) were mixed with 0.1 mL of tested EOs or vitamin C (reference). The plate was mixed by shaking, wrapped with aluminum foil, and then maintained for 30-60 min at 25 • C in a dark place. colors ranged from deep violet to light yellow, and the decrease in absorbance was measured at 517 nm. The DPPH was calculated from the following equation: where AC: The mean of absorbance of the negative control; AE: The mean of absorbance of extract sample or standard.

Molecular Response of Insect to Botanical Pesticides
The molecular mechanism of botanical EOs pesticides and their mode of action was evaluated by studying the transcriptional expression levels for proteasome subunit alpha type-5 (CL8), arylalkylamine N-acetyltransferase (CL1294), and cytochrome (CYP 4Q4) gene in S. oryzae adult to the applied essential oils using the quantitative real-time polymerase chain reaction (qRT-PCR) analysis.

Total RNA Isolation from Sitophilus oryzae
The three replicates from S. oryzae adult samples (2-7 days) (without treatment as control and other treated with three selected EOs) after 1 and 2 h exposure time of treatment were used for total RNA extraction using about 0.1 g from each replicate through using Tripure total RNA extraction reagent, according to the manufacturer's instructions. RNA concentration and purity were recorded by using NanoDrop™ 2000 (A260/A280).

Quantitative Real-Time -PCR (qRT-PCR)
qRT-PCR analysis was performed for three insecticidal detoxification genes (CL8, CL1294, and CYP4Q4) in S. oryzae adults treated with three selected EOs using SYBR Green Master Mix method utilizing a Rotor-Gene 6000 real-time PCR detection system (Qiagen, Germany). The primer sequences used in qRT-PCR mentioned in Table 1 were designed corresponding to the CL8, CL1294, and CYP4Q4 genes. β-Actin gene was used as an internal control gene. For each gene three replicates were used for qRT-PCR reaction; reaction volume of 25 µL contained 12.5 µL of 1×SYBR GREEN PCR mix (TaKaRa Code: RR041A), 1 µL 1:10 diluted cDNA templates, 1 µL sets of each primer and 9.5 µL Rnase free double distilled water according to the manufacturer's protocol. RT-qPCR was amplified in 3-step and 45 cycles at 95 • C for 15 s, and 60 • C for 30 s and 70 • C for 30 s. Data of qRT-PCR were collected as CT (PCR cycle number where fluorescence is detected above the threshold). The CT of each sample was used to calculate ∆CT values (target gene CT subtracted from 18sRNA gene CT). The relative gene expression of studied genes was determined using the 2 −∆∆Ct method [44,45]. Data from PCR runs were analyzed in the MyiQ optical system software version 1.0 (Bio Rad Laboratories Inc., Hercules, CA, USA). The expression quantity of expressed genes was calculated according to the Sigma Plot version 9.0 software (Systat Software Inc.: San Jose, CA, USA). Table 1. Nucleotide primer sequences used in qRT-PCR analysis in S. oryzae adult stage treated with essential oils.

Statistical Analysis
The results are presented as means ± standard deviation (±SD). Data were analyzed using the statistical package for social sciences (SPSS) 16 and were evaluated by analysis of variance (ANOVA). Duncan's test was used for comparisons among different treatments. Statistical differences were considered significant at the p < 0.05 level 3.

Preliminary Phytochemical Screening Analysis of EOs
According to preliminary phytochemical screening results of O. basilicum, N. sativa and L. angustifolia EOs, Table 2 shows the presence of phytochemical constituents in the selected botanical EOs with antioxidant activities such as glycosides in O. basilicum, N. sativa extracts, steroids, terpenoids, tannins and sterols compounds in O. basilicum, N. sativa and L. angustifolia EOs.

GC-MS Analysis of Essential Oils
In this study, qualitative analyses of basil, black seeds, and lavender EOs by using GC-MS showed the presence of many bioactive gradients, which may have a role in insecticidal effects. The peak area (%) and the retention time of the chemical compounds in the analyzed oils were enumerated in its chromatogram.

Analysis of O. basilicum (basil) EOs
Chemical analysis performed by GC-MS analysis of O. basilicum EOs was demonstrated in Scheme 1 that is characterized by the presence of many biologically important compounds listed in Table 3 such as linalool Eugenol, (1S)-camphor, Octamethylcyclotetrasiloxane and linalyl acetate compounds that are referenced with insecticide properties constituents.

Analysis of N. sativa (Black Seeds) EOs
Chemical analysis performed by GC-MS analysis of N. sativa EOs was illustrated in Scheme 2, which is characterized by the presence of many biologically important compounds listed in Table 4 such as α-Thujene, p-cymene, Palmitic acid, Linoleic acid, Erucic acid, and Trielaidin compounds that referenced with insecticide properties constituents.

Analysis of L. angustifolia (Lavender) EOs
Chemical analysis performed by GC-MS analysis of L. angustifolia EOs was represented in Scheme 3, which is characterized by the presence of many biologically important compounds listed in Table 5 such as hexanal, Eucalyptol, Lavandulyl acetate, Eugenol, Bicyclo[10.1.0]tridec-1-ene, Linoleic acid, and cis-11-Eicosenoic acid compounds that referenced with insecticide properties constituents.

Antioxidant Activity and Free Radical Scavenging Capacity
Among the non-enzymatic antioxidants parameters, the levels of total phenolic content, total flavonoid content, and free radical scavenging capacity (DPPH) were measured in the study and the data in Table 6 show significant contents of TPC and TFC and DPPH capacity in three studied EOs. The highest content from total phenol and total flavonoid was 31.4 mg GAE/g and 17.6 mg QE/g in basil EOs. Additionally, a maximum DPPH capacity 18.9% was reported in basil essential oil.

Mortality Bioassay against Adult
Overall, mortality percentages in S. oryzae adult stage increased with increasing concentrations (2, 4, and 6 mg/cm 2 ) and exposure time (3, 6, 12, 24, and 48 h) after using essential oils of basil, black seeds and lavender compared to control (10 ppm malathion).
For O. basilicum EOs, the obtained results represented in Figure 1 illustrated that the highest significant value of mortality 100% using concentration 6 mg/cm 2 at 48 h exposure time, compared to control (10 ppm malathion), followed by 92.2% at 6 mg/cm 2 after 24 h exposure time. On the other hand, there was no mortality in any of the untreated control. For N. sativa EOs, the data presented in Figure 2 showed that the highest significant mortality of S. oryzae adult stages was recorded at concentration 6mg/cm 2 at all exposure times. The highest percentage of mortality of S. oryzae was reported at 6 mg/cm 2 at 48 h with the value of 96.4% followed by the concentration 6 mg/cm 2 at 24 h with the value of 70.3% compared to control with the value 98.2%. For L. angustifolia EOs, the highest significant mortality of S. oryzae adult stages was represented in the highest concentration 6 mg/cm 2 at all exposure times. The highest percentage of S. oryzae mortality 100% was recorded at 6 mg/cm 2 after 48 and 24 h compared to their control values at 48 and 24 h were 100 and 98.2%, respectively, followed by 98.2 and 80.8% for 6 mg/cm 2 at 24 and 12 h, respectively ( Figure 3). Finally, after using three selected EOs against S. oryzae mortality; L. angustifolia EOs recorded the highest percentage of mortality 100% at exposure time 48 and 24 h, followed by O. basilicum recorded the percentage of mortality 100 and 98.2% at 48 and 24 h, respectively.

Repellant Bioassay against Adult
In general, the repellant percentage in S. oryzae adult stage increased with increasing the concentrations of three studied EOs and increasing exposure times. Regarding O. basilicum EOs results in Figure 4, the highest significant repellency of S. oryzae was recorded as 82.3% for 0.75 mg/cm 2 at 5 h compared to control (4 ppm malathion) at 5 h with the value of 93.2%, followed by 78.3 and 69.7% for 0.75 mg/cm 2 at 4 and 3 h, respectively, while the lowest significant repellency value was 54.2% at 0.25 mg/cm 2 O. basilicum after 1 h. For N. Sativa EOs, data represented in Figure 5 indicated that the highest significant repellency of S. oryzae adult stages was 77.5% at 0.75 mg/cm 2 after 5 h compared to its control at the same exposure time with the value of 93.2%, followed by 74.6 and 67.7% for 0.75 mg/cm 2 after 4 and 3 h, respectively, while the lowest significant repellency value was 45.6% at 0.25 mg/cm 2 EO after 1 h. For L. angustifolia EOs, obtained results in Figure 6 showed that the highest repellency percentage of S. oryzae adult stages increasing with increasing the concentration of EOs (0.25, 0.5, and at all exposure times). The highest significant repellence activity of L. angustifolia EO was recorded at 0.75 mg/cm 2 after 5 h with the value of 77.5% after 5 h versus its control (4 ppm malathion) was 93.2% after the same exposure time, followed by 74.6 and 67.8% for 0.75 mg/cm 2 after 4 and 3 h, respectively. While the lowest repellency value was 45.6% at 0.25 mg/cm 2 . On the other hand, the lowest significant repellency value was 45.6% at 0.25 mg/cm 2 L. angustifolia after 1 h. These results revealed that O. basilicum EOs recorded the highest repellence activity against S. oryzae with a value of 82.3% followed by L. angustifolia and N. sativa EOs.

Gene Expression Analysis
qRT-PCR analysis was performed at two different times after treatment (1 and 2 h) for three detoxification genes of Cytochrome P450 (CYP4Q4) and DEGs genes (CL8 and CL1294) in S. oryzae adult stage treated with O. basilicum, N. sativa, and L. angustifolia EOs as a natural insecticidal agent.
According to the qRT-PCR results in Figure 7, it showed maximum up-regulated expression level of CL8 gene exhibiting 6.08 fold changes in mRNA in S. oryzae adults treated with 6 mg/cm 2 concentration of L. angustifolia EOs for 2 h, followed by 4.76 fold at 10 ppm malathion after 2 h. The minimum expression level reached 1.73 fold in adult insects treated with (6 mg/cm 2 ) of N. sativa EOs for 1 h as compared with the reference gene (housekeeping gene, β-Actin). According to the qRT-PCR results, Figure 8 shows the maximum up-regulated expression level of CYP4Q4 cytochrome gene as 4.76 at 10 ppm malathion after 2 h changes in mRNA in S. oryzae adult treated with (6 mg/cm 2 ) for 2 h, followed by 3.75 fold change in adult insect treated with (6 mg/cm 2 ) of L. angustifolia EOs, and the minimum expression level reached 1.73 fold in adult insect treated with (6 mg/cm 2 ) of N. sativa EOs for 1 h as compared with reference gene (housekeeping gene, β-Actin). According to the qRT-PCR results, Figure 9 reports that the maximum up-regulated expression level of CL 1294 gene exhibits 8.32 fold changes in mRNA in S. oryzae adult treated with (6 mg/cm 2 ) of L. angustifolia EO for 2 h, followed by 4.76 at 10 ppm malathion after 2 h and the minimum expression level reached 2.08 fold in adult insect treated with (6 mg/cm 2 ) concentration of N. sativa EO for 1 h as compared with reference gene (housekeeping gene, β-Actin).

Discussion
This study assesses the toxicity effect of three selected EOs (basil, black seeds, and Lavender) against S. oryzae. The highest insecticidal activity was recorded using basil and lavender EOs with 100% mortality effect. Pesticide disinfection is the most important method for the protection of the stored cereals and grains against insects [46]. Several research studies showed insecticides and repellence activities of many EOs extracted from different wild, spice, and herb plants against several stored-product insect pests [47][48][49][50][51][52]. In this study, Lavender EO had the highest toxicity activity for rice weevils with 100% mortality effect at 6 mg/cm 2 at 12 and 48 h exposure time, in addition to the basil EO also had the highest mortality percentage 100% at 6 mg/cm 2 at 12 h, this result was in agreed with [53], who reported the toxicity effect of basil, fennel, and geranium EOs against S. oryzae and C. maculatus through assessment of repellence and progeny production. Quick repellence activity in this study was highest for basil EO that recorded 61.2% for 0.75 mg/cm 2 after 1 h against adult S. oryzae where repellence reached 82% after 5 h, theses result like results of [54], that reported repellence against S. oryzae after 1 h was 91.1%.
Active compounds in highest concentrations in basil EO were eugenol, linalool, estragole and methyl cinnamate, while active compounds in lavender were lavandulyl acetate, octacosane, and eugenol; black seeds characteristic by Limonene and 9, 12 Octadecadienoic acid active compounds. Eugenol active compound had repellent activity against Ixodes ricinus [55]. The estragole active compound had insecticide and pesticide activity against stored Vigna pest (Callosobruchus maculatus) [56]. Presences of estragole and t-anethole compounds in essential oil were an indicator for insecticide characters for this EO which had antimicrobial activity [57].
Eugenol is considered the important active compound in basil which had an effective effect against S. zeamais and T. castaneum, also had important fumigant activity with stored rice against the rice weevil [58]. The essential oils, especially basil and clove, can be used as an effective control agent for stored grain pests by fumigation. Active compounds in botanical EOs have some limitations such as low bioavailability, high volatility, and photodegradation that restrict their use on several occasions [59].
Previous reports are available on the fumigation activity related to various concentrations of plant EOs against pest insects S. zeamais [60] while the fumigant effect of investigated EOs in S. zeamais was enhanced by increasing the dose or exposure time of EOs, or different pest insects used [61,62]. The highest fumigant efficiency of clove and thyme EOs reported by [63], showed a 100% mortality of S. oryzae. Additionally, the results of [64] indicated that the EOs of clove caused 100% mortality similar to our result for basil and lavender Eos.
The repellant bioassay results in S. oryzae adults' response to EOs using filter paper method show strong repellant effect (98.1%) after 48 h at a dose (0.75 mg/cm 2 ) of L. angustifolia EOs, and 82.3% repellent after 48 h exposure to (0.75 mg/cm 2 ) of O. basilicum EOs, as compared with 93.2% repellency after 48 h of exposure to (4 ppm) of chemical insecticide malathion. The essential oil of O. basilicum was principally composed of the monoterpenoid as terpinene and the terpene with alcohol group in O. basilicum and N. sativa Eos, such as linalool and linalyl acetate, which are known to have repellent and toxic activities against stored product insect [65]. The main components obtained in O. basilicum oil were similar to those described by [66][67][68]. Our results are similar to the previous study, the insecticidal toxicity in different pests by exposure to different concentrations of C. cyminum and L. angustifolia EOs [69][70][71][72][73]. According to phytochemical analysis data, the insecticidal activity related to the presence of linalyl acetate and linalool and other bioactive compounds in investigated O. basilicum, N. sativa, and L. angustifolia EOs, our phytochemical results were similar to previous studies, showing strong insecticides and repellent activities toxicity of C. cyminum and L. angustifolia EOs against different stored-product insects related to the presence of linalyl acetate and linalool [49,74,75]. Moreover, the fumigant toxicity of 1,8-cineole and linalool have been investigated against B. germanica (L.) and O. surinamensis by Abdelgaleil et al. [76]. In addition, [77] showed acetylcholine esterase inhibition in S. oryzae adults and T. castaneum larvae related to 1,8-cineole and linalool exposure.
The effective potentiality of studied plant EOs due to the presence of the most toxic components for insects such as linalool and linalyl acetate for O. basilicum, linalool, methyl ether, linoleic acid ethyl ester for N. sativa and lavandulyl acetate for L. angustifolia similar to previous studies proved major constituents in essential oils with toxic activity and insecticidal potentialities such as limonene, camphor, 1, 8-cineole, and g-terpinene [78,79]. Studied EOS resulted in death for S. oryzae adult pest, and this may be because of neurotoxic effect for these plants Eos, as revealed previously, which medicinal volatile and aromatic wild plants contain essential chemical constituents acting as inhibitors for S. oryzae and other insects [80,81]. In this study, toxicity against S. oryzae through detecting the mortality percentage was highest using L. angustifolia EO with the value of 100% at 48 and 24 h, this result agrees with the result of [82], who reported the highest mortality against Sitophilus oryzae and Callosobruchus chinensis with the value of 100% after two days using Cinnamomum sieboldii bark extract. The highest Inhibition activity and toxicity were recorded using EOs of L. angustifolia and O. basilicum due to their plants are aromatic and contain volatile oils. In the same context, [83] ensured that oils with high volatility led to the decay of insects through their fumigant and gaseous actions. Our results are in harmony with [84], who reported that cinnamon EO had the most toxic effect at 1 h against S. zeamais. Gene CYPs is a metabolic component in microorganisms and insects and plants; which released against any stresses as insecticides, pesticides, fungicides, and herbicides through increasing the activity of flavonoids compounds and increasing the antioxidant activity.
Mode of action of CYPs occurs through increasing the content of CBT-ol which induces and exhibits resistance [85]. Results of qRT-PCR revealed the expression of selected genes CL8, CYP 4Q4, and CL1294 demonstrated the expression of these genes up and down in each treatment. Mode of action for detoxification genes inside insects has three pathways [86]. For pathway 1 functional group as nucleophilic was infused to the xenobiotic compound which active and considered as a water-soluble compound, the gene of cytochrome CYP 4Q4 is important in phase I. These genes have the main role in the detoxification and metabolization of insecticides leading to reduce toxic effect [87]. For phase II, enzymes increase solubility for water for the metabolite of Phase I through union with endogenous compound, which prevents tissue damage through combination with molecules of insecticides and CL1294 important in this phase [88]. Finally, in phase III, ABC enzyme transport xenobiotic compound out of the cell [89].

Conclusions
In conclusion, the highest repellence effects against S. oryzae were recorded by using basil EO with all concentrations and at different exposure times. This effect is related to the chemical composition of basil essential oil containing eugenol, linalool, and estragole. Lavender and basil EOs have the highest mortality effect against rice weevils. Expression of detoxification system of S. oryzae genes was increased in case of using lavender and basil EO. This study recommends using basil and lavender EOs as insecticides and pesticides against S. oryzae as biological control.