Ethnobotanical Research and Compilation of the Medicinal Uses in Spain and the Active Principles of Chiliadenus glutinosus (L.) Fourr. for the Scientific Validation of Its Therapeutic Properties

The species Chiliadenus glutinosus (L.) Fourr. has a large number of therapeutic uses reported in the traditional Spanish medicine. The growing interest in preserving the ethnopharmacological knowledge related to the botanical diversity existing in Spain and the interest in achieving scientific validation of the therapeutic properties of medicinal species has led to the development of this study. To do it, all the known medicinal uses of Ch. glutinosus in Spain were compiled, then an exhaustive bibliographic research on its chemical composition was carried out, and finally, an in silico validation of the bioactive phytochemicals present in a higher proportion in the essential oil of Ch. glutinosus: camphor, borneol, lucinone, glutinone, quercetin, kutdtriol, and kaempferol; in an attempt to justify the reported traditional uses of the species. It was found that much of the traditional medicinal uses of Ch. glutinosus, along with the biological activity of its phytochemicals, are supported by scientific evidence. The results place this species in a prominent position to initiate possible lines of research to develop new, more effective drugs and improve therapies to treat conditions and diseases that affect the different organic systems of the human being.


Introduction
The genus Chiliadenus Cass. belongs to the Asteraceae family. This genus is composed of at least ten endemic species of certain areas of the Mediterranean region. In Spain lives a single species of this genus, Chiliadenus glutinosus (L.) Fourr., whose distribution in the Eastern Spanish mainland also extends through the island of Mallorca, southern France, and northern Morocco [1][2][3][4][5][6].
Medicinal plants have been used by mankind since ancient times as a remedy for all kinds of ailments and conditions, especially human remedies. However, Chiliadenus glutinosus, popularly known as "té de roca" (rock tea), "té de Aragon" (Aragon tea), or "arnica" [7], has not been referenced for its medicinal uses in past centuries by botanists such as Laguna [8], Clusius [9,10], or Quer y Martínez [11]. Palau y Verdera [12] cites it by a synonym in the 18th century, without mentioning any traditional use; and at the end of the 19th century, the Spanish botanists Loscos Bernal and Pardo Sastrón [13,14], and also the German, Gadow [15], collected the first references on the therapeutic uses of the species. This plant is also not listed in the Royal Spanish Pharmacopoeia [16]. This contrasts with its known and widespread use today and in recent decades in those places in Spain where the species grows naturally, mainly in some regions of the eastern half of the peninsula [1,17].
Chiliadenus glutinosus is a plant with a wide range of medicinal applications in Spanish popular culture. Remedies based on this species have been used to treat problems of the It is a perennial plant with a size between 10 and 45 cm with a simple or branched stem, and abundant trichomes, some short glandular (up to 0.5 mm), and others long non-secreting (0.2-5 mm). It has a narrow rhizome with long and thin roots that are introduced between the rocks. The leaves are 10-25 × 2-5 mm in size and are lanceolate, sharp, erect-patent and, covered with glandular hairs. The flower heads are 7-15 mm wide and are gathered in corymbose or cymose inflorescences, sometimes also solitary. The involucre is 8-15 mm long and has bracts in several rows. The external bracts (3-5 × 0.5-1 mm) are herbaceous, secretory, and considerably shorter than the internal ones. The internal bracts (5-10 × 0.7-1 mm) are scarious, ciliated, and non-glandular. In each flower head, there are between 55 and 105 yellow florets, and there are no ray florets. The cypsela is pubescent with glandular hairs at the apex. The pappus has two rows of hairs, the outer one with short hairs (0.5-1 mm) and the inner one with longer hairs (5-7.5 mm), denticulate, and reddish-brown ( Figure 2). It is a basophilic species, lives exclusively in between 55 and 105 yellow florets, and there are no ray florets. The cypsela is pubescent with glandular hairs at the apex. The pappus has two rows of hairs, the outer one with short hairs (0.5-1 mm) and the inner one with longer hairs (5-7.5mm), denticulate, and reddish-brown ( Figure 2). It is a basophilic species, lives exclusively in fissures and cracks of sunny calcareous crags at between 350 and 1000 meters of altitude, and its flowering takes place between July and August [7,[29][30][31].

Compilation of the Medicinal Uses of Chiliadenus glutinosus
The medicinal uses of the species Chiliadenus glutinosus in Spanish culture have been compiled by reviewing specific literature and are set out in the following Tables 1 to 7, arranged into Regions (the Spanish Autonomous Community). The part used to prepare these remedies is usually the flowering top of the plant, sometimes the part used is not specified.

Medicinal Uses Applied to the Human Circulatory System
Ten different medicinal uses of Chiliadenus glutinosus have been compiled aimed at treating different disorders of the human circulatory system in six Autonomous Communities. Its use is mentioned in the form of an infusion to regulate blood pressure, in Catalonia with a hypertensive effect and in north of Andalusia with a hypotensive effect. In Aragon, southeast of Castilla-La Mancha and north of Andalusia, the infusion is

Compilation of the Medicinal Uses of Chiliadenus glutinosus
The medicinal uses of the species Chiliadenus glutinosus in Spanish culture have been compiled by reviewing specific literature and are set out in the following Tables 1-7, arranged into Regions (the Spanish Autonomous Community). The part used to prepare these remedies is usually the flowering top of the plant, sometimes the part used is not specified.

Medicinal Uses Applied to the Human Circulatory System
Ten different medicinal uses of Chiliadenus glutinosus have been compiled aimed at treating different disorders of the human circulatory system in six Autonomous Communities. Its use is mentioned in the form of an infusion to regulate blood pressure, in Catalonia with a hypertensive effect and in north of Andalusia with a hypotensive effect. In Aragon, southeast of Castilla-La Mancha and north of Andalusia, the infusion is taken to give fluidity to the blood and improve its circulation. This infusion is also used to treat other related problems, such as purifying the blood in Navarra, relieving pain in swollen legs, or improving circulation in north of Valencian Community and Region of Murcia, and fighting anemia in Region of Murcia. Finally, it is recommended to take this infusion to

Medicinal Uses Applied to the Human Digestive System
A total of eleven medicinal uses of Chiliadenus glutinosus have been collected from thirteen Spanish Autonomous Communities, aimed at treating conditions of the digestive system. In some areas of Navarre and Catalonia, the plant is left to macerate in anise for some time and the resulting liquid is drunk as a digestive drink after meals. Another of the most popular uses of the plant is its use as a digestive infusion in a wide territory comprised of several regions: east of Catalonia, Aragon, Navarre, southern Basque Country, La Rioja, southeast of Castile and Leon, southeast of Castilla-La Mancha, center and south of Valencian Community, Cantabria, and towns in the northeast and southeast of Andalusia. Ch. glutinosus infusion is used as a remedy for diarrhea in north of Aragon, Navarre, southern Basque Country, north and center of Valencian Community, and north of Andalusia; for appendicitis in north of Aragon, and to relieve stomach pain in Navarre, southern Basque Country), center of Valencian Community, Community of Madrid, and southeast of Castilla-La Mancha. In other towns of belonging to southeast of Castilla-La Mancha, Region of Murcia, and north of Andalusia it is recommended to take this infusion for several days to cure stomach ulcers; in center of Valencian Community and north of Andalusia it is also used to eliminate gasses, and in Region of Murcia, it is recommended to reduce the feeling of heaviness in the stomach. This infusion is also used to stimulate the appetite in center of Valencian Community and Region of Murcia or to induce vomiting in high doses in north of Valencian Community. In other regions of Spain such as south of Aragon, east of Castilla-La Mancha, center of Valencian Community, northeast and southeast of Andalusia or the Balearic Islands, the infusion is recommended in a more non-specific way for all types of stomach problems and indispositions (Table 2).

Medicinal Uses Applied to the Human Genitourinary System
The five medicinal uses of Chiliadenus glutinosus compiled to treat the genitourinary system are prepared in infusion, in the four different Autonomous Communities. The infusion of this plant is taken to help urinate more frequently, since it is considered to be diuretic, in towns in Region of Murcia, southeast of Castilla-La Mancha, and Cantabria. Moreover, in Region of Murcia and southeast of Castilla-La Mancha, it is recommended to take this infusion when suffering from kidney pains or to promote the elimination of kidney stones. Finally, in some towns of Region of Murcia and north of Andalusia, it is recommended to drink the infusion to treat kidney diseases in general and to "improve the kidney" by taking the infusion preventively to avoid possible infections, kidney stones, etc. (Table 3). Catalonia [1] Infusion Catalonia [36] Aragon [18,37] Navarre [38][39][40] Basque Country [22,41,42] La Rioja [43] Castile and Leon [44] Valencian Community [45][46][47][48] Castilla-La Mancha [49,50] Andalusia [33,51,52] Cantabria [53] Diarrhea Infusion Aragon [54] Navarre [40] Basque Country [22,41,42] Valencian Community [1,[45][46][47] Andalusia [17] Appendicitis Aragon [54] Stomach ache Navarre [34,55] Basque Country [22,41,42] Valencian Community [45][46][47] Community of Madrid [56] Castilla-La Mancha [57] Stomach ulcers Region of Murcia [35] Castilla-La Mancha [58] Andalusia [17,33] Gasses Valencian Community [1,46] Andalusia [33] Indigestion or heaviness Region of Murcia [35] Stimulate the appetite Valencian Community [47] Region of Murcia [35] Digestive problems or indisposition (in general) Aragon [54,59] Valencian Community [47,48] Region of Murcia [35] Balearic Islands [23] Castilla-La Mancha [49,57,58] Andalusia [21,32] Help to vomit Infusion (high doses) Valencian Community [1]  Three medicinal uses of Chiliadenus glutinosus have been compiled in three Autonomous Communities. The plant is applied as a decoction, macerated in alcohol, and as an infusion to treat conditions of the locomotor system, mainly to combat rheumatic and bone pain. In locations of north of Andalusia, the plant is cooked, and the resulting liquid is applied in the form of baths in the joint areas where pain is suffered, and in the Region of Murcia, the fresh plant is collected and left to macerate in alcohol for some time. Then, that alcohol is applied in the form of rubbing to relieve sore areas from rheumatism. Finally, the plant is also used as an internal infusion to relieve body fatigue in southeast of Castilla-La Mancha (Table 4).

Medicinal Uses Applied to the Human Nervous System
A total of four medicinal uses of Chiliadenus glutinosus have been compiled in infusion form, in five Autonomous Communities, aimed at treating conditions of the human nervous system. In some localities of northwest and northeast of Catalonia, center of Valencian Community and southeast of Castilla-La Mancha, the plant is prepared in infusion and it is taken to calm the nerves. In Navarre, other properties are attributed to it and this infusion is considered to lift mood and clear the mind; also, in Navarre and some municipalities of east of Castilla-La Mancha and north of Andalusia, it is recommended to drink this infusion to relieve headaches (Table 5). Table 5. Compilation of the medicinal uses of Chiliadenus glutinosus as a remedy for diseases of the nervous system in Spain, compiled from the existing specific literature.

Remedy or Use Form of Employment Administration Autonomous Community
The nerves

Infusion Internal
Catalonia [1] Valencian Community [45] Castilla-La Mancha [1] Lift mood Navarre [34,55] Clear the mind Navarre [34,55] Headache Navarre [60] Castilla-La Mancha [49] Andalusia [32] 2.2.6. Medicinal Uses Applied to the Human Respiratory System For the respiratory system, six medicinal uses of Chiliadenus glutinosus have been compiled, using the plant in an infusion, decoction, or vapors, in eight Autonomous Communities, as a remedy for diseases of the human respiratory system. The use of the plant in infusion has been reported in numerous towns in northwest and east of Catalonia, Aragon, Navarre, center of Valencian Community, Region of Murcia, southeast of Castilla-La Mancha, north of Andalusia, and Cantabria, as a good remedy for colds and flu. Other applications of this infusion are to relieve a sore throat in northwest of Catalonia, Region of Murcia, and north of Andalusia; treat bronchitis in southeast of Castilla-La Mancha and north of Andalusia; or improve asthmatic processes in southeast of Castilla-La Mancha. Another of the reported uses of this plant has been the use of the vapors resulting from its decoction as a remedy to open the lungs in cases of asthma in localities of southeast of Castilla-La Mancha. In the Balearic Islands, the decoction or infusion of the plant is also taken to treat respiratory infections in general (Table 6). Table 6. Compilation of the medicinal uses of Chiliadenus glutinosus as a remedy for diseases of the respiratory system in Spain, compiled from the existing specific literature.

Remedy or Use Form of Employment Administration Autonomous Community
Colds and flu

.2.7. Medicinal Uses Applied to the Human Integumentary System
A total of five medicinal uses of Chiliadenus glutinosus have been compiled, applied in the form of poultices, washes, ointments, or plasters. These uses have been reported in eight Autonomous Communities and have been aimed at treating conditions of the integumentary system. The fresh leaves of the plant have been applied in a poultice as a remedy for skin pimples in Navarre. Other reported uses are the use of the species as an infusion or decoction to wash and disinfect wounds and bruises in Region of Murcia and southeast of Castilla-La Mancha; the preparation of a healing ointment with the plant to apply also on wounds and bumps on the skin, and in the form of a decoction to relieve the discomfort caused by the insect bites such as mosquitoes in Albacete. In other locations in southeast and east of Castilla-La Mancha, plasters are prepared to treat skin burns or the resulting decoction is applied to them; also, in north of Andalusia, the species is used in the form of decoction or infusion to reduce inflammation of skin wounds (Table 7).  [24][25][26]. In the present study, it has been decided to select and analyze the following seven phytochemicals: camphor, borneol, lucinone, glutinone, kutdtriol, quercetin, and kaempferol, from the total of compounds, as they are present in greater proportions in the essential oil of Ch. glutinosus. The study of the biological activity of the selected phytochemicals could contribute to support or refute the traditional medicinal uses of the species in Spain. Hereunder, Figure 3 below shows the selected compounds, the chemical class to which they belong, and their structure in a two-dimensional format.

Experimental Studies on the Biological Activity of Phytochemicals Present in Chiliadenus glutinosus
The Table 8 shows the seven selected compounds present in greater proportions in the essential oil of Chiliadenus glutinosus and their biological activities, which could explain the reported medicinal uses of the plant in Spain. Among the compounds listed in Table 8, the anti-inflammatory, antioxidant, and antibacterial activities stand out, proven in clinical trials, of the camphor and borneol monoterpenes, and the quercetin and kaempferol flavonols. Table 8. Biological activity of the compounds in higher proportions present in the essential oil of Chiliadenus glutinosus.

Chemical Group
Phytochemical Biological Activity

In Silico Modeling for the Prediction of Bioactive Compound Targets
The Swiss Target Prediction software [102] was used to estimate the macromolecular targets most likely to bind to the bioactive compounds camphor, borneol, lucinone, glutinone, kutdtriol, quercetin, and kaempferol, present in Chiliadenus glutinosus. This tool statistically quantifies the probability that two similar bioactive molecules share their Plants 2021, 10, 584 10 of 21 protein targets. Therefore, when performing a query on the possible binding targets of a specific bioactive molecule, the tool displays and arranges the possible molecular targets from highest to lowest binding probability, up to a maximum of one hundred targets. At times, there can be a 100% binding probability. In these cases, the similarity is total. Therefore, it is likely that the experimental bioactivity of the consulted molecule is known, and it is not a real prediction. What can also happen is that no objective is obtained in the prediction. On this occasion, the input molecule is below the thresholds of similarity in 2D and 3D with any of the known active molecules against which were examined and compared.

Camphor
The camphor molecule was introduced into the Swiss Target Prediction [102] simulator, which assumes it is a bioactive molecule and calculates the binding targets. As a result, 22 camphor-binding targets were obtained in Homo sapiens, with binding probabilities between 4% and 20%. The first six results shown in Table 9 have a probability of between 10% and 20% of being targets of the camphor molecule. Of all the targets obtained, the nuclear receptor of subfamily 1, group I, member 3 (20%), cytochrome P450 (19%), carbonic anhydrases I, II, and IV (15%), and an androgen receptor (10%) showed the highest probability of being targets of the camphor molecule. Data for targets with binding probabilities of less than 10% are not shown.

Borneol
Twenty-eight binding targets were obtained in the case of the borneol molecule, in Homo sapiens, with binding probabilities between 4% and 35%. The first 12 results obtained have a probability of between 10% and 35% of binding to the borneol molecule. In this case, only the first four results with the highest probability of binding to the borneol are shown in Table 10, which shows probabilities between 31% and 35%. The rest of the targets with binding probabilities lower than 30% are not shown. The first results show as main borneol, the carbonic anhydrases I, II, and IV (36%), and a transient receptor potential cation channel, subfamily M, member 8 (31%).

Glutinone
In the case of the glutinone molecule, 32 binding targets were obtained in Homo sapiens, with binding probabilities between 12% and 100%. The first three results shown in Table 11 have been selected since they have a probability of between 23% and 100% of being targets of the glutinone molecule. The remaining results for targets with binding probabilities less than 23% are not displayed. Of all the targets analyzed, cycloxigenase 1 (100%), cytochrome P450 (54%), and cathepsin D (23%), showed the highest probability of being glutinone binding targets.

Quercetin
For the quercetin molecule, 100 binding targets were obtained in Homo sapiens, with binding probabilities between 21% and 100%. The first 67 results have a 100% join probability. In Table 12, the first six targets, NADPH oxidase 4, the vasopressin receptor V2, aldose reductase, xanthine dehydrogenase, monoamine oxidase A, and the insulin-like growth factor I receptor, all with a 100% binding probability. The rest of the objectives are not shown.

Kaempferol
In the case of the kaempferol molecule, 100 binding targets were obtained in Homo sapiens, with binding probabilities between 17% and 100%. The first 17 results have a binding probability of 100%. In Table 13, the first six targets have been selected as examples: NADPH oxidase 4, aldose reductase, xanthine dehydrogenase, tyrosinase, tyrosine-protein kinase receptor FLT3, and carbonic anhydrase II. All these targets showed a 100% binding probability with the kampferol, the rest of the results are not shown. Finally, it is worth mentioning that the simulations with the lucinone and kutdtriol molecules did not give results, so it has not been possible to predict the binding targets of these compounds in Homo sapiens.

Discussion
Chiliadenus glutinosus is a well-known and commonly used plant for medicinal purposes for decades until the present day throughout eastern Spain. Numerous uses have been reported to treat different pathologies and conditions of the different systems of the human body: the circulatory, digestive, genito-urinary, locomotive, nervous, respiratory, and integumentary systems [103][104][105][106].

Circulatory System
Chiliadenus glutinosus has been used as an infusion in localities of Jaen to treat hypotension. This blood pressure-raising effect can be associated with compounds such as camphor. The effect of this phytochemical was tested on patients with orthostatic hypotension [70]. The study suggests that the underlying hemodynamic mechanisms can be attributed to an increase in total peripheral resistance induced by an increase in the tone of the arterioles, thereby counteracting an orthostatic drop in blood pressure. This infusion has also been used with the opposite effect as a remedy for hypertension in Catalonia. It has been proven in other clinical trials that daily oral administration of quercetin in rats with induced hypertension, progressively reduces systolic pressure, which is why antagonistic effects are observed for different compounds present in the same species [79].
In other regions such as Aragon, southeast of (Castilla-La Mancha or north of Andalusia, the infusion of this plant is considered to be useful to give greater fluidity and achieve better blood circulation. It has been proven that some compounds such as borneol and quercetin have antithrombotic or anti-aggregate effects, which reduce the risk of cardiovascular diseases and thrombi. The antithrombotic effect of borneol could be due to its anticoagulant effect [78]. Flavonoids such as quercetin have also been reported to inhibit collagen-induced platelet aggregation in vitro. However, in vitro concentrations may not have the same effect as in vivo [80,108]. The Chiliadenus glutinosus infusion has also been used in north of Valencian Community, Region of Murcia, and southeast of Castilla-La Mancha to reduce the pain of swollen legs or improve circulation in people who suffer from varicose veins. Camphor and borneol have been shown in human clinical trials to have anti-inflammatory effects. In addition, camphor can increase blood flow locally in skin and muscle, in turn providing sensations of cold and heat, which achieves an effect of improving blood circulation and could give some relief to the aforementioned conditions [69,73]. The main mechanism proposed for the antiinflammatory effect of borneol could be based on the fact that this compound significantly decreased the release of interleukins IL-8 and IL-6 (molecules involved in inflammatory processes [109]), with an average inhibition of 67-76% and 50-61% respectively, although other similar compounds could also have a synergistic influence. Camphor has also been shown to activate transient specific calorific potential receptors TRPV1 and TRPV3, and the human and rat cold-sensitive channel transient receptor (TRPM8). According to the prediction of the Swiss Target Prediction software [102] (Tables 9 and 10), camphor can bind to carbonic anhydrase I (CA1), an enzyme responsible for the regulation of cellular pH and mediator of cerebral vascular permeability, and borneol that is known having an affinity for the TRPM8 receptor, and sensitive to temperature changes.
The infusion of this plant has also been recommended in the traditional medicine of southeast of Castilla-La Mancha) and north of Andalusia to improve the weak heart and its ailments. Trials with quercetin have been shown to have a positive effect on the recovery of myocardial contractile activity, especially after an ischemic process. This mechanism appears to be related to cytochrome P450; quercetin induces a decrease in xanthine dehydrogenase (XDH) and xanthine oxidase (XO), typical enzymes of ischemic processes [85]. The simulation in Swiss Target Prediction [102] also shows the affinity of the quercetin molecule for the XDH enzyme (Table 12), which contributes to the generation of reactive oxygen species.
Reviewing other articles, evidence of other beneficial effects on the circulatory system of some compounds present in Chiliadenus glutinosus has also been found. The possible antidiabetic effect of kaempferol and quercetin stands out, being able to significantly improve insulin-stimulated glucose absorption in mature adipocytes [91]. Quercetin is also mentioned as a possible preventive compound against arteriosclerosis [85] or the possible neuroprotective and vasodilator effect of kaempferol [97,101]. However, no scientific evidence has been found linking the use of Ch. glutinosus infusion in localities of Murcia as a treatment for anemia. According to the prediction in Swiss Target Prediction [102], quercetin and kaempferol have affinity for the enzyme NADPH oxidase 4 (NOX4), which can regulate the insulin signaling cascade (Tables 12 and 13). Therefore, there could be a certain relationship between these compounds and their possible anti-diabetic effect, although a more exhaustive study would be necessary to know the mechanisms involved.

Digestive System
The infusion of Chiliadenus glutinosus is also widely used in much of the eastern regions of the peninsula as a remedy to treat diarrhea. This effect could be based on the spasmolytic action of quercetin [87], which blocks voltage-gated calcium channels and relaxes precontracted tissues in in vivo assays with KCl depending on the concentration. The anticonvulsant effect of borneol has also been reported in rats, causing an inhibitory effect on electrically or chemically inducible seizures [110].
Another of the best-known uses of this infusion is to treat stomach ulcers, especially in southeast of Castilla-La Mancha, Region of Murcia, and north of Andalusia. The most common cause of this type of ulcer is the bacterium Helicobacter pylori (Marshall et al., 1985;Goodwin et al., 1989), which can be found in the human gastrointestinal tract. The compound borneol has antimicrobial properties. It is thought that its mechanism of action is based on the disruption of the integrity of the bacterial membrane [72,111]. Other reported properties of borneol are its analgesic and anti-inflammatory effect, due to its action on the modulation of the GABA system involved in pain regulation, which could be associated with the treatment of common stomach complaints [74]. Reviewing other articles, the in vitro cytotoxic effect of camphor on human epithelial colorectal carcinoma cells has also been reported [112]. However, it has not been possible to verify or find evidence that relates the compounds present in the infusion of the plant, with the digestive, emetic, and appetite stimulant effects reported in several regions or their use to treat appendicitis in north of Aragon).

Genito-Urinary System
Traditionally, the infusion of Chiliadenus glutinosus was used to treat some conditions related to the genito-urinary system. In some towns in Region of Murcia and southeast of Castilla-La Mancha, the infusion is consumed to relieve kidney pain. This use may be related to the anti-inflammatory and analgesic effect of borneol and camphor, already discussed [69,70]. Quercetin and kaempferol could also provide anti-inflammatory effects. The infusion was reported in the Region of Murcia, as a remedy for kidney diseases (e.g., urinary infections caused by microorganisms) This use could be related to the antimicrobial activity of the compounds present in Chiliadenus glutinosus: camphor and borneol, together with quercetin and kaempferol [72,82,90,97,113]. Other reported uses of this infusion were as a diuretic drink in Cantabria, southeast of Castilla-La Mancha, and Region of Murcia to treat kidney stones, although no related trials or scientific evidence have been found to justify these applications. However, the Swiss Target Prediction software [102] shows as a molecular target of quercetin, the vasopressin arginine receptor (AVPR2), involved in water reabsorption in the kidneys (Table 12). So, this relationship should be studied in a way which the two components interact, and their effects on kidney function.

Locomotor System
In Region of Murcia and north of Andalusia the external application of Chiliadenus glutinosus decoction has been recommended to relieve bone pain and rheumatism. This application could be associated with the anti-inflammatory effects of compounds such as lucinone, glutinone, kutdtriol, and quercetin, or analgesic effects related to camphor and borneol [25,69,70,82]. The Swiss Target Prediction [102] software shows the glutinone affinity for the enzyme cyclooxygenase 1 (PTSG1), which enables the production of prostaglandins from arachidonic acid (Table 11). For this reason, it would be necessary to know the interaction of both elements and their effects on inflammatory processes. It can also be observed in Table 9, the affinity of camphor for carbonic anhydrase 2 (CA2), essential in the processes of bone resorption and differentiation of osteoclasts.

Nervous System
The infusion of Chiliadenus glutinosus has been used as a remedy to treat states of nervousness, elevate mood, and clear the mind in Catalonia, southeast of Castilla-La Mancha, center of Valencian Community, and Navarre. Some compounds present in Ch. glutinosus such as borneol, have shown sedative or anxiolytic effects that could justify the above uses. Trials were performed in mice that were induced pain with acetic acid injection and hot plate, and the results corroborated the sedative effect of borneol, due to its mentioned action on the modulation of the GABA system [74]. This infusion has also been used to treat headaches in east of Castilla-La Mancha and north of Andalusia), which could be justified by the analgesic effect of borneol, an important central and peripheral antinociceptive, and anti-inflammatory [74]. In other studies, its topical analgesic effect has been proven, relieving pain in patients with postoperative pain, since this compound induces a topical analgesia mediated by the cationic cold receiving channel TRPM8 and a descending glutamatergic mechanism in the spinal cord. Another reported effect of borneol is the neuroprotective effect. The blood-brain barrier (BBB) plays an essential role in maintaining a stable homeostatic environment, so its destruction or increased permeability are common pathological processes in many serious diseases of the central nervous system (CNS). In another study, the administration of borneol exerted a significant decrease in the permeability of the blood-brain barrier, keeping it at stable levels and having a preventive effect on possible CNS pathologies or cerebral ischemic lesions [77].

Respiratory System
Chiliadenus glutinosus infusion has also been commonly recommended throughout the eastern regions of the peninsula to treat colds and flu. This could be associated with the antimicrobial and viricidal effect of borneol [72,111]. For example, its effectiveness has been proven against herpes simplex virus 1 (HSV-1), the results showed a complete inhibition of viral replication at a concentration of 0.06%. Kaempferol is also an effective antiviral, it has been proven effective against the herpes simplex virus, cytomegalovirus, influenza virus (responsible for seasonal flu), and the human immunodeficiency virus (HIV). Kaempferol inhibits the reverse transcriptase enzyme and viral and binding proteases, stopping the viral replication of the mentioned viruses [97,114].
The infusion has also been used to treat bronchitis, asthma, or sore throat, which could be due to the anti-inflammatory and stimulating effect of camphor and borneol on the respiratory system [69,74,76].
Other trials [85,90] show that quercetin administration reduced the incidence of upper respiratory tract infections in cyclists after intensified outdoor exercise in winter and a growing literature base supports the anti-pathogenic properties of quercetin against rhinovirus, adenovirus, and coronavirus related to these respiratory infections. These studies indicate that quercetin blocks viral replication at an early stage of multiplication using several mechanisms, including inhibition of molecular coupling proteases, binding of proteins to the viral capsid, and suspension of virulence enzymes such as DNA gyrase and cell lipase [90].

Integumentary System
The infusion or decoction of Chiliadenus glutinosus has been used topically to reduce inflammation of skin pimples in Navarre. The anti-inflammatory effect may be due to the presence of lucinone, glutinone, and kutdtriol. These compounds showed antiinflammatory activity in mouse peritoneal macrophages and inhibited cyclooxygenase 1 (PTSG1), reducing the production of prostaglandin 2 (PGE2), involved in inflammatory processes [25,74]. The Swiss Target Prediction [102] software also reveals the affinity of glutinone for PTSG1 (Table 11). The liquid resulting from the decoction of the plant has been used externally to disinfect wounds, blows or animal bites in Region of Murcia, southeast and east of Castilla-La Mancha), and north of Andalusia), which may be related and justified to the antimicrobial, antinociceptive, and anti-inflammatory properties of the borneol, and the topical sensation of cold provided by camphor, which can provide relief from these conditions [73].

Other Biological Activities of Interest Present in the Selected Compounds.
Reviewing the information and results of different clinical trials [25,72,77,79,84,94,115], other biological properties of the selected compounds have been found, less related to the therapeutic uses of Chiliadenus glutinosus in folk medicine, but of great interest for the research and development of future treatments for numerous human pathologies.
Firstly, borneol, camphor, kaempferol, and quercetin have antioxidant properties. It has been proven in vitro tests that the administration of borneol can reduce oxidative reactions and the toxicity of free radicals by increasing the activity of superoxide dismutase (SOD), a powerful antioxidant enzyme that plays a bio-protective role by alternatively catalyzing the dismutation of the superoxide radical in molecular oxygen or hydrogen peroxide in living cells [72,77]. According to other studies, kaempferol has antioxidant and protective effects against endothelial damage. Its mechanism of action may be associated with an improvement in nitric oxide production and a decrease in dimethylarginine levels [25,94]. Quercetin and its glycosylated derivatives are also capable of inhibiting lipid peroxidation in various biological systems in vitro: lipoproteins, liposomes, hepatocytes in cell cultures, and erythrocyte membranes [79,82,84,115].
Furthermore, it is worth mentioning the antiproliferative activity of quercetin. This cytotoxic action could be related to the incapacity to release lactate, the reduction of the nucleotide 5'-adenosine triphosphate (ATP), the stimulation of the production of transforming growth factor-beta 1 (TGF-b1), known for its antiproliferative activity, and blocking potassium channels. Quercetin can also prevent cancer cells from developing cellular thermotolerance, and therefore a greater efficacy could be achieved through anticarcinogenic treatment by clinical hyperthermia [80,116]. Secondly, there are studies that corroborate the antigenotoxic effect of borneol on rats' hepatocytes and testicular cells when it is administered orally in drinking water. It was verified that borneol reversed the oxygen and glucose deprivation followed by reperfusion-induced neuronal injury, nuclear condensation, the production of reactive intracellular oxygen species (ROS), and dissipation of mitochondrial membrane potential in cortical neurons [25]. For all these reasons, it is considered of interest to deepen the study of the antioxidant and antiproliferative mechanisms of these molecules, to develop better treatments and drugs against cancer.

Sources of Information
Firstly, an exhaustive bibliographic search was carried out to compile the medicinal uses of the Chiliadenus glutinosus species in Spain, the chemical composition, and previous clinical trials on the biological activity of some phytochemicals present in the species. For this, specialized databases such as Dialnet, Google Scholar, Pub Med, or ScienceDirect, were used. Keywords were used to search for information such as "traditional uses", "ethnobotany", "traditional medicine", combined with the correct species name "Chiliadenus glutinosus", or its synonymous plant names such as "Jasonia glutinosa", "Jasonia saxatilis (Lam.) Guss.", "Erigeron glutinosus L.", or "Inula saxatilis Lam.", and finally the names of the provinces or Autonomous Communities of Spain where the plant lives were added to these search terms. Finally, the names of the molecules present in the species such as "borneol" or "camphor", were also used as keywords. The chemical structures of some molecules present in Chiliadenus glutinosus were obtained from the public domain PubChem database [117], maintained by the National Center for Biotechnology Information (NCBI) of the United States.

Distribution: Data Collection and Mapping
The Chiliadenus glutinosus distribution map was drawn up by downloading from the GBIF biodiversity data portal [28], and the references of the presence of the species in Spain. With this information, a distribution map of the species was elaborated with the ArcGIS software version 10.5.1 [27].

Validation Methodology
Finally, in silico modeling was performed using the Swiss Target Prediction online tool [102] to predict the targets of the bioactive molecules present in Chiliadenus glutinosus. This tool is based on the so-called "principle of similarity", which establishes that two similar molecules also have similar properties. Therefore, when introducing a bioactive molecule into the tool to query the possible targets to which it can be attached, a prediction is made based on the combination of 2D and 3D similarity between a set of approximately 370,000 assets in known targets. Once the query is made, the tool displays and arranges the possible molecular targets from highest to lowest probability of binding.

Conclusions
After the corresponding review of specific literature on previous clinical trials and the in silico predictions of possible molecular targets of the phytochemicals present in higher proportions in the essential oil of Chiliadenus glutinosus, suggest the biological activities related to the compounds present in the plant, and outline its potential which must be confirmed by clinical studies of these majority compounds. Therefore, the potential properties provided by the presence of camphor borneol, quercetin, kaempferol lucinone, glutinone, and kutdtriol in Ch. glutinosus, establish a link between the therapeutic effects to a large part of the reported uses of the species to treat several conditions of the organic systems of the human being in traditional Spanish medicine.
The results place the species in a promising position to suggest future scientific research. This should focus on conducting in vitro and in vivo clinical trials testing the extracts and all the phytochemical components of the plant to gain a better understanding of their biological activity, their effects on the body, the mechanisms of action, the most appropriate form of administration and the effective doses to achieve beneficial effects on human health, which would provide a direct and much more comprehensive validation of its medicinal properties.
To conclude, this study contributes to the preservation, documentation, and validation of the medicinal uses of Chiliadenus glutinosus in Spain and lays the foundations for future scientific research to develop new drugs and more effective therapies to treat conditions and diseases that affect the different organ systems of the human being and opens a new way to promote the cultivation and production of the species for medicinal purposes at an industrial level.