Mixtures of Biological Control Agents and Organic Additives Improve Physiological Behavior in Cape Gooseberry Plants under Vascular Wilt Disease

This study aimed to assess the soil application of mixtures of biological control agents (BCAs) (Trichoderma virens and Bacillus velezensis) and organic additives (chitosan and burnt rice husk) on the physiological and biochemical behavior of cape gooseberry plants exposed to Fusarium oxysporum f. sp. physali (Foph) inoculum. The treatments with inoculated and non-inoculated plants were: (i) T. virens + B. velezensis (Mix), (ii) T. virens + B. velezensis + burnt rice husk (MixRh), (iii) T. virens + B. velezensis + chitosan (MixChi), and (iv) controls (plants without any mixtures). Plants inoculated and treated with Mix or MixChi reduced the area under the disease progress curve (AUDPC) (57.1) and disease severity index (DSI) (2.97) compared to inoculated plants without any treatment (69.3 for AUDPC and 3.2 for DSI). Additionally, these groups of plants (Mix or MixChi) obtained greater leaf water potential (~−0.5 Mpa) and a lower MDA production (~12.5 µmol g−2 FW) than plants with Foph and without mixtures (−0.61 Mpa and 18.2 µmol g−2 FW, respectively). The results suggest that MixChi treatments may be a promising alternative for vascular wilt management in cape gooseberry crops affected by this disease.


Introduction
Cape gooseberry (Physalis peruviana L.) is a plant species belonging to the Solanaceae family and its center of origin is the Andean region of South America [1]. This fruit has acquired economic importance due to its high content of vitamins A, C, and B, essential minerals such as iron (Fe), phosphorus (P), potassium (K), and zinc (Zn), and antioxidants (tocopherols, carotenoids, and ascorbic acid) [2,3]. This species was cultivated in 976 ha obtaining a production of 12,152 t in Colombia in 2019 [4]. Likewise, cape gooseberry ranks second in the list of most exported fruits in the country, with Colombia being the first largest producer in the world followed by South Africa [3,5].
Vascular wilt caused by Fusarium oxysporum is one of the main limitations in economically important Andean fruit trees such as lulo (Solanum quitoense Lamarck.) and cape gooseberry [5][6][7]. This disease caused by Fusarium oxysporum f. sp. physali (Foph) is the greatest limitation in cape gooseberry production in Colombia. Vascular wilt generates a considerable decrease in production and yield per hectare, going from 19,300 t and 18 t ha −1 in 2009 to 16,100 t and 12.2 t ha −1 for 2018, respectively [4,5,8].
The fungus is characterized by affecting plants at any phenological stage. The main symptoms of the disease are root rot, chlorosis of the borders and central parts of mature leaves, loss of turgor in young leaves and stems, stunted growth, and finally death of the tomato [48], root rot (Cylindrocarpon destructans and Fusarium solani) in ginseng [47], and downy mildew (Pseudoperonospora sp.) in bitter gourd (Momordica charantia L.) [49].
The combination of BCAs and organic additives may be of interest for disease control in different crops [50,51]. In this sense, the use of the mixture of Bacillus pumilus and chitosan in tomato plants inoculated with F. oxysporum f. sp. radicis-lycopersici obtained promising results in the management of the disease due to increased root resistance to the infection [52].
Plants face many biotic agents (such as viruses, bacteria, fungus, or arthropods), causing biotic stress in their hosts. These agents can disrupt normal metabolism, plant growth, and yield [53]. In Colombia, one of the most limiting biotic agents in cape gooseberry crops is Foph, which is the species that causes vascular wilt, showing a wilt incidence greater than 50% in the production areas [54]. Alternatives based on biological control have become very important for the P. peruviana-Foph pathosystem in Colombia [11,38,45]. These studies have allowed selecting promising alternatives and knowing the plant responses to the individual use of BCAs such as T. virens or B. velezensis. They have also allowed the evaluation of the effect of applications of organic additives such as chitosan or burnt rice husk on vascular wilt management and cape gooseberry plant physiology. However, information on the joint activity of these control tools (BCAs + organic additives) on the disease and its effect on plant physiology remains scarce. Therefore, this research aimed to study the comparative response of the application of three mixtures of BCAs and additives (i) T. virens + B. velezensis (Mix), (ii) T. virens + B. velezensis + burnt rice husk (MixRh) or (iii) T. virens + B. velezensis + chitosan (MixChi) on the plant processes (biochemistry, photosynthetic machinery, water status, and growth) of Foph-inoculated and uninoculated cape gooseberry seedlings.

Estimation of Vascular Wilt Development by AUDPC, Disease Index and Vascular Browning
Disease incidence in Foph + inoculated plants was 100%. Isolates in PDA medium allowed confirming the pathogen's presence in symptomatic plants and inoculated with Foph + , and its absence was also confirmed in non-inoculated (Foph − ) cape gooseberry plants ( Figure 1). Differences between treatments in the AUDPC (p = 0.0058) and disease severity index (p = 0.0189) were observed at 50 DAI (Table 1). Cape gooseberry seedlings without the addition of the mixtures and inoculated with Foph + (pathogen control) registered the highest AUDPC values (69.3) (Table 1; Figure 1A). Intermediate values for the AUDPC were observed in plants with the application of the mixture of T. virens + B. velezensis and the addition of burnt rice husk (MixRh) (63.8) (Table 1; Figure 1C). Finally, the lowest values were recorded for the mixtures T. virens + B. velezensis (Mix) and T. virens + B. velezensis with the addition of chitosan (MixChi) (56.6 and 57.6, respectively) (Table 1; Figure 1B-D).  3 7.28 4.67 6.47 MixChi/Foph+ 57.6 b 2.95 ab 4.02 bc Significance ** 2 * *** CV (%) 3 7.28 4.67 6.47 1 Values followed by different letters in the same column are significantly different from p ≤ 0.05 according to the Tukey's test; 2 *, ** and *** Significant at p ≤ 0.05, p ≤ 0.01 and p ≤ 0.01, respectively; 3 C.V.: Coefficient of variation. The lowest severity index values were registered in seedlings with the application of the mixture of T. virens + B. velezensis (Mix) (2.83), while higher severity values were obtained in the pathogen control (Foph + ) (3.20). Finally, the vascular browning percentage registered similar results to those obtained in the AUDPC. The lowest values of vascular browning were evident in Foph-inoculated plants (Foph + ) treated with the mixtures T. virens + B. velezensis (Mix) (3.66) and T. virens + B. velezensis with the addition of chitosan (MixChi) (4.02) ( Table 1). This trend can be observed in Table 1 and Figure 1A, where the greatest values of vascular browning were registered in pathogen control plants (Foph + ) compared to inoculated seedlings treated with the different mixtures and plants of the absolute control (Foph) (Figure 1B-D).

Growth Parameters
Growth parameters (total dry weight (TDW), leaf area (LA), and leaf area ratio (LAR) of cape gooseberry seedlings displayed differences (p = 0.0000, p = 0.0000, and p = 0.0000, respectively) in the interaction between the presence of Foph and the mixtures (Mix, MixRh, and MixChi) at 50 DAI. The group of plants without pathogen inoculation (Foph − ) registered the highest growth parameters compared to plants inoculated with the pathogen (Foph + ) ( Figure 2). Regarding TDW, the application of the different mixtures (Mix, MixRh, and MixChi) favored this variable in inoculated (Mix 3.1 g, MixRh 3.6 g, and MixChi 3.8 g) and non-inoculated plants (Mix 3.8 g, MixRh 5.5 g, and MixChi 5.3 g) ( Figure  2A). LA was also favored by all mixture treatments in both inoculation situations (with and without Foph), registering the highest values with the application of T. virens + B. velezensis (Mix) in inoculated (682.1 cm 2 ) and non-inoculated (810.2 cm 2 ) plants ( Figure  2B). Finally, the application of T. virens + B. velezensis (Mix) also increased (199.2 cm 2 ·g −1 ) The lowest severity index values were registered in seedlings with the application of the mixture of T. virens + B. velezensis (Mix) (2.83), while higher severity values were obtained in the pathogen control (Foph + ) (3.20). Finally, the vascular browning percentage registered similar results to those obtained in the AUDPC. The lowest values of vascular browning were evident in Foph-inoculated plants (Foph + ) treated with the mixtures T. virens + B. velezensis (Mix) (3.66) and T. virens + B. velezensis with the addition of chitosan (MixChi) (4.02) ( Table 1). This trend can be observed in Table 1 and Figure 1A, where the greatest values of vascular browning were registered in pathogen control plants (Foph + ) compared to inoculated seedlings treated with the different mixtures and plants of the absolute control (Foph) (Figure 1B-D).

Growth Parameters
Growth parameters (total dry weight (TDW), leaf area (LA), and leaf area ratio (LAR) of cape gooseberry seedlings displayed differences (p = 0.0000, p = 0.0000, and p = 0.0000, respectively) in the interaction between the presence of Foph and the mixtures (Mix, MixRh, and MixChi) at 50 DAI. The group of plants without pathogen inoculation (Foph − ) registered the highest growth parameters compared to plants inoculated with the pathogen (Foph + ) ( Figure 2). Regarding TDW, the application of the different mixtures (Mix, MixRh, and MixChi) favored this variable in inoculated (Mix 3.1 g, MixRh 3.6 g, and MixChi 3.8 g) and non-inoculated plants (Mix 3.8 g, MixRh 5.5 g, and MixChi 5.3 g) (Figure 2A). LA was also favored by all mixture treatments in both inoculation situations (with and without Foph), registering the highest values with the application of T. virens + B. velezensis (Mix) in inoculated (682.1 cm 2 ) and non-inoculated (810.2 cm 2 ) plants ( Figure 2B). Finally, the application of T. virens + B. velezensis (Mix) also increased (199.2 cm 2 ·g −1 ) LAR values mainly in diseased plants compared to the same group of inoculated plants and treated with MixRh and MixChi (~157.7 cm 2 ·g −1 ) ( Figure 2C).

Stomatal Conductance and Leaf Water Potential
The stomatal conductance (g s ) and leaf water potential (Ψ wf ) are summarized in Figure 3. Differences were also observed between Foph inoculation and treatments with mixtures on g s (p = 0.0000) and Ψ wf (p = 0.0000) at 50 DAI. In general, cape gooseberry plants with Foph + showed lower g s compared to non-inoculated plants (~79.2 mmol m −2 s −1 and~280.7 mmol m −2 s −1 , respectively). However, the application of treatments with the different mixtures (Mix, MixRh, and MixChi) favored g s compared to inoculated plants (Foph + ) without application of mixtures (~90 mmol m −2 s −1 and 47.6 mmol m −2 s −1 , respectively) ( Figure 3A). The Ψ wf displayed similar trends to those recorded for g s . The group of non-inoculated plants (Foph − ) showed a higher water status (Ψ wf~− 0.32 Mpa) compared to Foph + plants (Ψ wf~− 0.53 Mpa). However, the application of different mixtures favored Ψ wf in Foph + inoculated plants by 17% ( Figure 3B).

Stomatal Conductance and Leaf Water Potential
The stomatal conductance (gs) and leaf water potential (Ψwf) are summarized in Figure 3. Differences were also observed between Foph inoculation and treatments with mixtures on gs (p = 0.0000) and Ψwf (p = 0.0000) at 50 DAI. In general, cape gooseberry plants with Foph + showed lower gs compared to non-inoculated plants (~79.2 mmol m −2 s −1 and ~280.7 mmol m −2 s −1 , respectively). However, the application of treatments with the different mixtures (Mix, MixRh, and MixChi) favored gs compared to inoculated plants (Foph + ) without application of mixtures (~90 mmol m −2 s −1 and 47.6 mmol m −2 s −1 , respectively) ( Figure 3A). The Ψwf displayed similar trends to those recorded for gs. The group of noninoculated plants (Foph − ) showed a higher water status (Ψwf ~ −0.32 Mpa) compared to Foph + plants (Ψwf ~ −0.53 Mpa). However, the application of different mixtures favored Ψwf in Foph + inoculated plants by 17% ( Figure 3B). F. oxysporum f. sp. physali (Foph) inoculation at 50 days after inoculation (DAI). Error bars represent the mean of four values ± standard error. Significant differences between treatments are indicated by different letters according to the Tukey's test (p ≤ 0.05). ***p < 0.001-values of the ANOVA of Foph inoculation, mixture treatments, and their interaction.

Chlorophyll and Carotenoid Content
Total chlorophyll (TChl) and carotenoid (Cx + c) contents are shown in Figure 4A F. oxysporum f. sp. physali (Foph) inoculation at 50 days after inoculation (DAI). Error bars represent the mean of four values ± standard error. Significant differences between treatments are indicated by different letters according to the Tukey's test (p ≤ 0.05). *** p < 0.001-values of the ANOVA of Foph inoculation, mixture treatments, and their interaction.

Chlorophyll and Carotenoid Content
Total chlorophyll (TChl) and carotenoid (Cx + c) contents are shown in Figure 4A

Malondialdehyde and Proline Content
Differences (p = 0.0000) were recorded between inoculation with Foph and the treatment with mixtures for the variable's proline content and lipid membrane peroxidation expressed as MDA content at 50 DAI ( Figure 4C,D). The lowest levels of proline content were registered in pathogen control plants (Foph + ) (99.91 µmol g −2 FW). The use of the different mixtures (Mix, MixRh, and MixChi) promoted proline synthesis, with the mixtures T. virens + B. velezensis (Mix) and T. virens + B. velezensis with the addition of chitosan (MixChi) being those that generated the greatest proline accumulation (262.86 µmol g −2 FW and 256.86 µmol g −2 FW, respectively) ( Figure 4C). In contrast, lower MDA values were recorded with the application of the different mixtures, mainly with T. virens + B. velezensis (Mix) (11.9 µmol g −2 FW) and T. virens + B. velezensis with the addition of chitosan (MixChi) (13.1 µmol g −2 FW) compared to pathogen control plants (Foph + ) (18.2 µmol g −2 FW) ( Figure 4D). The lowest values of lipid peroxidation were observed in Foph − seedlings, especially with the Mix treatment (8.9 µmol g −2 FW) ( Figure 4D).

Efficacy of Mixtures of Biological Control Agents (BCAs) and Organic Additives and Relative Tolerance Index (RTI)
The highest values of percentage of efficacy were recorded in plants treated with the mixtures T. virens + B. velezensis (Mix) and T. virens + B. velezensis with the addition of chitosan (MixChi) (17% and 11.8%, respectively) ( Figure 5A). The previous analysis is also

Malondialdehyde and Proline Content
Differences (p = 0.0000) were recorded between inoculation with Foph and the treatment with mixtures for the variable's proline content and lipid membrane peroxidation expressed as MDA content at 50 DAI ( Figure 4C,D). The lowest levels of proline content were registered in pathogen control plants (Foph + ) (99.91 µmol g −2 FW). The use of the different mixtures (Mix, MixRh, and MixChi) promoted proline synthesis, with the mixtures T. virens + B. velezensis (Mix) and T. virens + B. velezensis with the addition of chitosan (MixChi) being those that generated the greatest proline accumulation (262.86 µmol g −2 FW and 256.86 µmol g −2 FW, respectively) ( Figure 4C). In contrast, lower MDA values were recorded with the application of the different mixtures, mainly with T. virens + B. velezensis (Mix) (11.9 µmol g −2 FW) and T. virens + B. velezensis with the addition of chitosan (MixChi) (13.1 µmol g −2 FW) compared to pathogen control plants (Foph + ) (18.2 µmol g −2 FW) ( Figure 4D). The lowest values of lipid peroxidation were observed in Foph − seedlings, especially with the Mix treatment (8.9 µmol g −2 FW) ( Figure 4D).

Efficacy of Mixtures of Biological Control Agents (BCAs) and Organic Additives and Relative Tolerance Index (RTI)
The highest values of percentage of efficacy were recorded in plants treated with the mixtures T. virens + B. velezensis (Mix) and T. virens + B. velezensis with the addition of chitosan (MixChi) (17% and 11.8%, respectively) ( Figure 5A). The previous analysis is also confirmed by Figure 1 where pathogen control plants (Foph + ) showed the greatest

Comparative Analysis of Vascular Wilt Mitigation by the Application of Mixtures of Biological Control Agents (BCAs) and Organic Additives
Correlations between some disease monitoring and physiological variables (AUDPC, vascular browning, Ψwf, and proline content) and RTI showed that treatments with the mixtures T. virens + B. velezensis (Mix) and T virens + B. velezensis with the addition of chitosan (MixChi) mitigated the negative effect generated by the inoculation of the pathogen. These treatments (Mix, MixChi, and pathogen control (Foph + )) were compared to the group of seedlings without inoculation of the pathogen (Foph − ) and without any mixture treatment (absolute control) at 50 DAI. These results showed that applications of T. virens + B. velezensis (Mix) and T. virens + B. velezensis with the addition of chitosan (MixChi) helped plants to cope with the Foph inoculation condition since a positive effect of these mixtures was observed on gs, Ψwf, TDW, LA, TChl, Cx + c, MDA, and proline content (Figure 7). The three-dimensional graph (percentage of efficacy, proline, and gs) confirmed the correlations and comparative analysis described above. The three-dimensional analysis among physiological, biochemical, and disease monitoring variables showed that applications of mixtures of BCAs or BCAs + organic additives (Mix or MixChi) can decrease the levels of vascular wilt and could be considered for both the response against pathogens or the mitigation of stress conditions by promoting plant physiological processes ( Figure  8). 60 70 80  Proline (μmol·g

Comparative Analysis of Vascular Wilt Mitigation by the Application of Mixtures of Biological Control Agents (BCAs) and Organic Additives
Correlations between some disease monitoring and physiological variables (AUDPC, vascular browning, Ψ wf, and proline content) and RTI showed that treatments with the mixtures T. virens + B. velezensis (Mix) and T virens + B. velezensis with the addition of chitosan (MixChi) mitigated the negative effect generated by the inoculation of the pathogen. These treatments (Mix, MixChi, and pathogen control (Foph + )) were compared to the group of seedlings without inoculation of the pathogen (Foph − ) and without any mixture treatment (absolute control) at 50 DAI. These results showed that applications of T. virens + B. velezensis (Mix) and T. virens + B. velezensis with the addition of chitosan (MixChi) helped plants to cope with the Foph inoculation condition since a positive effect of these mixtures was observed on g s , Ψ wf , TDW, LA, TChl, Cx + c, MDA, and proline content (Figure 7). The three-dimensional graph (percentage of efficacy, proline, and g s ) confirmed the correlations and comparative analysis described above. The three-dimensional analysis among physiological, biochemical, and disease monitoring variables showed that applications of mixtures of BCAs or BCAs + organic additives (Mix or MixChi) can decrease the levels of vascular wilt and could be considered for both the response against pathogens or the mitigation of stress conditions by promoting plant physiological processes (Figure 8).

Discussion
Positive effects on the management of vascular wilt have been reported separately for the application of BCAs (Trichoderma or Bacillus) [26,36,55] and organic additives such as chitosan [46,56]. However, recent reports on the use of these compounds in mixtures

Discussion
Positive effects on the management of vascular wilt have been reported separately for the application of BCAs (Trichoderma or Bacillus) [26,36,55] and organic additives such as chitosan [46,56]. However, recent reports on the use of these compounds in mixtures

Discussion
Positive effects on the management of vascular wilt have been reported separately for the application of BCAs (Trichoderma or Bacillus) [26,36,55] and organic additives such as chitosan [46,56]. However, recent reports on the use of these compounds in mixtures for the management of vascular wilt are still scarce [52,57,58]. In this study, the different mixtures (Mix, MixRh, or MixChi) exerted control over the disease. This was mainly observed for treatments with T. virens + B. velezensis (Mix) or T. virens + B. velezensis with the addition of chitosan (MixChi), which showed lower AUDPC, severity index, and vascular browning values in cape gooseberry seedlings (Table 1; Figure 1). Izquierdo-García et al. [26] also observed that the use of a mixture of T. virens and B. velezensis lowered vascular wilt (Foph) severity in cape gooseberry. Bakeer et al. [59] similarly observed that the joint application of chitosan with T. harzianum and B. suptilis reduced Fusarium wilt severity in tomato (Solanum lycopersicum L.) plants by 71% compared to plants without treatments. The application of combinations of BCAs and organic additives can also benefit plants by promoting better growth and development, greater association with the soil microbial community, and higher effectiveness in the control of pathogens [60].
Fusarium oxysporum infection causes direct negative effects such as lower the leaf gas exchange properties, dry matter, plant water status, and photosynthetic pigments, and a higher MDA and proline production [12][13][14][15]. This research showed that the applications of mixtures of BCAs and organic additives (Mix, MixRh, or MixChi) helped plants to cope with the negative effects caused by Foph on their physiological and biochemical responses. The variables g s , Ψ wf , TDW, LA, photosynthetic pigments, and MDA and proline contents were positively affected by the treatments mainly with T. virens + B. velezensis (Mix) or T. virens + B. velezensis with the addition of chitosan (MixChi). Okorski et al. [61] obtained similar responses with the application of a mixture of different BCAs (biological preparation of effective microorganisms such as lactic acid and photosynthetic bacteria and yeast), which also generated an increase in gas exchange parameters such as photosynthesis, g s, and transpiration in pea plants (Pisum sativum L.) with Fusarium oxysporum wilt symptoms. A previous study also indicated that Foph-infected cape gooseberry plants and treated with chitosan showed better gas exchange (g s ) and Ψ wf parameters compared to plants without chitosan [45].
Vascular wilt also causes changes in the distribution of plant assimilates and reduces water and nutrient transport which is reflected in lower growth [62]. However, in the present study growth (TDW, LA, and LAR) was favored in plants inoculated and treated with the different mixtures, with similar or superior behavior than that of absolute control plants (Foph − ) (Figures 2 and 7). Zaim et al. [63] also recorded an increase in plant height, root length, and fresh and dry matter of shoots and roots in chickpea plants inoculated with F. oxysporum f. sp. ciceris with the joint application of BCAs B. subtilis and T. harzianum. Likewise, applications of chitosan in mixture with B. subtilis and T. harzianum have shown higher crop yield of 61% compared to diseased tomato plants without the application of these mixtures [59].
The contents of MDA, proline, and leaf photosynthetic pigments can be used as biochemical markers to quantify the plant's response to biotic stress conditions [64][65][66]. Treatments mainly with Mix and MixChi favored the concentration of photosynthetic pigments (TChl and Cx + c) and proline, and decreased MDA accumulation in Fophinfected plants (Figures 4 and 7) in this study. A higher concentration of photosynthetic pigments (TChl and Cx + c) and proline has also been reported in lettuce (Lactuca sativa L.) plants infected with Rhizoctonia solani and with the application of a mixture of two bio fungicides formulated with T. harzianum and B. subtilis [67]. On the other hand, treatments with the mixture of three plant growth-promoting rhizobacteria (PGPR) of the genus Pseudomonas and chitosan significantly increased (>65%) chlorophyll content (SPAD) in tomato plants infected with tomato leaf curl virus (ToLCV) [68]. Likewise, Zhang et al. [69] registered a decrease in MDA production after treatment with the mixture of chitosan and the antagonistic yeast Rhodotorula mucilaginosa in strawberry (Fragaria ananassa Duch.) plants inoculated with Rhizopus stolonifer and Botrytis cinerea.
In this study, it was also found that treatments with mixtures of BCAs and organic additives such as chitosan helped to ameliorate the effects caused by Foph through the improvement of the water potential, stomatal behavior, and biochemical expression and the decrease of vascular wilt. This response may be caused by the fact that BCAs such as T. virens may participate in the activation of single or multiple biocontrol mechanisms against plant diseases, including the production of hydrolytic enzymes such as β-1,3-glucanases, chitinases and proteases (mycoparasitism), segregation of iron-chelating siderophores to suppress pathogen growth (competition), and production of secondary metabolites for resistance induction [22,70,71]. B. velezensis can contribute to the antagonistic action against pathogens through antibiosis and direct competition for the secretion of different secondary metabolites with antibacterial and antifungal activity (lipopeptides) in the rhizosphere. It can also benefit the host plant microbiome and stimulate induced systemic resistance (ISR) mediated by the production of elicitors such as jasmonic and ethylene salicylic acids [72][73][74].
The beneficial effect of BCAs on the water status and gas exchange properties (g s ) of plants could be related to a greater and better mineral availability in the soil. This may improve nutrient uptake and movement in plants, the efficient use of water, and the overexpression of proteins such as aquaporins that improve water and solute transport [61,75,76]. BCAs can synthesize growth hormones such as indole-3-acetic acid and gibberellic acid that promote plant growth and increased nutrient uptake through the production of secondary metabolites [77,78]. Additionally, the application of BCAs can regulate the biosynthesis of proteins and chlorophyll in plants (activation of porphobilinogen synthase enzyme) [79]. Finally, the positive results of BCAs application could be associated with increased activity of antioxidant enzymes (catalase, superoxide dismutase, and ascorbate peroxidase), and the induction of proline metabolism, which decreases the levels of lipid peroxidation of membranes [80,81].
Chitosan treatments decreased vascular wilt severity in cape gooseberry plants since they may play a role in the induction of plant defense, the activation of enzymes such as chitinases and β-1,3-glucanase, the biosynthesis of phytoalexin, the generation of reactive oxygen species, and the synthesis of inhibitors of callose and protease that affect fungal growth [39,82]. Furthermore, this biopolymer shows elicitor activity through antimicrobial activity (production secondary metabolites such as phenolic compounds), induction of systemically acquired plant resistance against a wide range of pathogens [42], and has mucoadhesive properties that improve permeation and can prolong the positive effects of compounds with chitosan [41]. Additionally, the use of chitosan generated an increase in g s , Ψ wf, and growth of cape gooseberry plants, probably due to the promotion of root development, the increase in water and nutrient uptake, the stimulation of osmotic adjustment which facilitates the accumulation of compatible solutes, and the regulation of processes such as elongation and division of cells, activation of enzymes, and synthesis of proteins under stress conditions [83][84][85]. Finally, chitosan treatments also favored the biochemical behavior (photosynthetic pigments, MDA, and proline) of diseased cape gooseberry plants. These responses could be related to the protection of the photosynthetic complex from protein and lipid oxidative damage in the chloroplast [86], the reduction of oxidative stress caused by chitosan's ability to bind with proteins and macromolecules, metal ions and negatively charged lipids, and the induction of free amino acid accumulation (proline) related to the osmotic adjustment and antioxidant defenses of stressed plants [87].
The use of burnt rice husk in the mixture with BCAs had a lower effect on vascular wilt control and the physiological and biochemical responses of cape gooseberry plants compared to the Mix and MixChi treatments (Table 1; Figures 1-8). Araujo et al. [88] showed that the application of a mixture of charcoal (biochar) and T. harzianum inhibited mycelial growth of Macrophomina phaseolina and stimulated the germination percentage, number of pods, and dry and fresh matter of bean (Phaseolus vulgaris L.) plants. The positive response to the application of this type of compounds (charcoal) may be related to the promotion of beneficial microorganisms' growth, the improvement of nutrient solubilization and uptake, the neutralization of phytotoxic compounds in the soil and the induction of plant defense mechanisms [89,90].
It is convenient to indicate that the external spores in the parts that remain above ground level (field conditions) are dispersed by the wind, water, people and equipment, and by the movement of soil particles that contain the fungus, hence the importance and benefit of considering the presence of Fusarium wilt in commercially important crops and favorable environmental conditions such as soil, climate, management, agronomic, among others [91]. Our results could serve as a basis for future lines of research by pioneers in Plant Protection sciences and biotic interactions in Colombia and others countries, where Fusarium wilt has been a big issue in crop protection of different crops such as banana (TR4) [92,93]. These findings also be a fundamental contribution to avoid the spread and devastation of plantations of commercially important crops such as Cape gooseberry.

Microorganisms and Culture Conditions
Strain Map5 of F. oxysporum f. sp. physali (Foph) and the BCAs Trichoderma virens and Bacillus velezensis were provided by the Microorganisms Collection of Corporación Colombiana de Investigación Agropecuaria-AGROSAVIA. Foph inoculum at an initial concentration of 1 × 10 6 microconidia·mL −1 was grown for 7 days on sterile potatodextrose broth (PDB, Difco ® ) under continuous agitation (125 rpm) at 25 • C. The fermented broth was then filtered using three layers of sterile muslin cloth and centrifuged at 15,000 rpm for 15 min. The obtained biomass was rinsed twice with sterile distilled water (SDW). The microconidia obtained were re-suspended in SDW, adjusting the suspension at 1 × 10 6 microconidia·mL −1 using a Neubauer chamber for counting. T. virens was grown for seven days on potato-dextrose-agar (PDA) and conidia were harvested by scraping with SDW to obtain the inoculum, which was also adjusted by Neubauer chamber count to 1x10 6 microconidia·mL −1 . B. velezensis was grown in Luria Bertani broth (LB, Tryptone 10 g, NaCl 10 g and yeast extract 5 g·L −1 ) at 25 • C using an orbital shaker at 125 rpm for continuous agitation for 48 h. The bacterial suspension concentration was adjusted by using a spectrophotometer (BIOTEK ® , Winooski, VT, USA) to measure the optical density (OD 600 nm = 1 × 10 8 cells. mL −1 ).

Plant Material and Growth Conditions
An experiment was carried out in the greenhouses of the Faculty of Agricultural Sciences of the Universidad Nacional de Colombia, Bogotá campus (4 • 35 56 N, 74 • 04 51 W, altitude 2557 m) between February and June 2017. The climatic conditions during the study were as follows: a natural photoperiod of 12 h (photosynthetically active radiation (PAR) 1500 µm −1 s −2 at noon), day/night temperature of 25/20 • C, and relative humidity of~72%. Commercial seeds (Semicol S.A., Bogotá, Colombia) of cape gooseberry ecotype 'Colombia' (highly susceptible to vascular wilt) [19,38], were subjected to superficial disinfection by immersion in a 70% ethanol solution (v/v) for 1 min, 3% sodium hypochlorite (v/v) for 20 min with agitation, and three washes using sterile distilled water. Additionally, the seeds destined for chitosan application were immersed in a chitosan solution (0.1% p/v) with constant agitation at 150 rpm for 20 min.
After disinfection, seeds sown in 70-cell germination trays using nutrient-free peat (Klasmann ® , Klasmann-Deilmann GmbH, Germany) as substrate. Thirty days after sowing (DAS) (seed germination), 10 mL of liquid compound fertilizer (N, P, K, and micronutrients) (Nutriponic ® , Walco S.A., Bogotá, Colombia) was used to irrigate seedlings at a concentration of 3 mL per liter of water every 3 days until transplantation (45 DAS). When four fully expanded leaves were observed in the seedlings, they were transplanted into 2 L plastic pots containing the appropriate substrate based on the treatment and Foph presence or absence.

Treatments with or without Foph Inoculation and the Addition of Mixtures of Biological Control Agents (BCAs) and Organic Additives
The microorganisms selected for the mixtures were Trichoderma virens and Bacillus velezensis (formerly B. amyloliquefaciens) due to their biocontrol potential against plant pathogens [11,38]. Regarding organic additives, chitosan (Sigma Aldrich, St. Louis, MO, USA) and burnt rice husk were also selected due to their potential control of Foph observed in a previous study [45]. Eight groups of treatments were obtained for the development of the experiment: (i) cape gooseberry plants with no addition of BCAs or organic additives and inoculated with Foph (pathogenic control (Foph + )) or without it (absolute control (Foph − )); (ii) cape gooseberry plants with or without Foph inoculation and with the application of the mixture of BCAs (T. virens and B. velezensis (Mix)); (iii) cape gooseberry plants with or without Foph inoculation with the application of the mixture of BCAs and the addition of chitosan (MixChi) and (iv) cape gooseberry plants with or without Foph inoculation with application of the mixture of BCAs and the addition of burnt rice husk (MixRh). Plants of treatments 1, 2, and 3 were established in substrate that contained a mixture of soil and rice husk at a 3:1 ratio (v/v), whereas burnt rice husk was incorporated into the soil at the time of transplantation obtaining a soil-husk substrate at a 3:1 ratio (v/v) in treatment 4. These substrate mixtures have been used in previous studies in which the individual effect of BCAs or organic additives on vascular wilt in cape gooseberry plants was compared [38,45]. Finally, the pathogen's absence in the soil used in the preparation of substrate mixtures was confirmed previously by the technique described by Park [94], by adding 100 mL of cool-molten galactose-nitrate agar (GNA) medium with 10 µg·mL −1 benomyl and 300 µg·mL −1 chloramphenicol to the soil sample. F. oxysporum colonies were counted after incubation for 7 days at room temperature.
The treatments with BCAs were carried out using a mixture of the suspensions of T. virens (1 × 10 6 conidia mL −1 ) and B. velezensis (1 × 10 8 cells mL −1 ) in sterile distilled water (SDW). For this, the Petri dish was scrapped to harvest T. virens conidia, and the PDA medium on which the fungus grew was liquefied in Ultra-Turrax ® adding 15 mL of SDW per Petri dish to obtain the fungus supernatant. The suspension was centrifuged (15,000 rpm, 15 min, 4 • C) and the obtained supernatant was filtered by 0.22 µm filters (Sartorius ® ). The fermented broth of B. velezensis was centrifuged (under the conditions described before) to separate the biomass from the supernatant. The supernatant was harvested and filtered using 0.22 µm filters and B. velezensis biomass was rinsed twice with SDW to remove any residues of supernatant [26]. Five milliliters of the combined suspension was applied in drench to each cell in the germination trays (30 DAS), and 30 mL was applied to each of the pots at the time of transplantation (at 45 DAS) for Mix and MixRh treatments, respectively. The mixture of BCAs and chitosan (MixChi) was performed as follows: (i) a first application of chitosan was carried out at seed disinfection, (ii) then, BCAs were drench-applied using 5 mL of the combined suspension (T. virens and B. velezensis) in germination trays (30 DAS), and (iii) a combined application of BCAs (15 mL) and chitosan (15 mL) was performed at the time of transplantation (45 DAS). The concentration used for chitosan applications was 0.1% (w/v) at both moments.
Fusarium oxysporum f. sp. physali inoculation was performed at transplantation by incorporating propagules (microconidia) into the substrate [5]. In this regard, for each 1.0 kg of substrate used, 100 mL of SDW was added with or without the presence of F. oxysporum f. sp. physali strain Map5 (highly virulent) microconidia [11]. Eight treatment groups were arranged in a completely randomized design with each treatment consisting of eight plants (replicates). Finally, the experiment lasted 95 days.

Disease Severity Analysis
The disease was evaluated by visual inspection of plants using the six-level scale created by Moreno-Velandia [95]; this scale considered the characteristic symptoms of the disease (epinastic response, chlorosis, turgor loss in leaves and plant defoliation until total wilting). For each of the treatments, disease severity was determined every 3 days after inoculation (45 DAS) until the end of the trial. Equation (1) proposed by Chiang et al. [96] was used to calculate the severity index.
Disease severity index = ∑ (nv)/V (1) where n represents the level of affectation based on the scale, v is the number of plants present at each level, and V is the total number of assessed plants.
The area under the disease progress curve (AUDPC) was determined in each treatment to obtain the severity of the disease using the trapezoidal integration method [97]: where n is the number of assessments, y i and y i+1 are the values of the severity scale obtained at each evaluation moment, and (t i+1 − t i ) is the time between assessments. Isolates on Potato Dextrose Agar (PDA) medium from explants collected from the stem base confirmed the pathogen's presence or absence in Foph + inoculated or non-inoculated plants (Foph) [98]. Vascular browning was evaluated 50 days after inoculation (DAI) in cross-sections of the stem base in each treatment. The vascular browning percentage was quantified using a five-level scale proposed by Mandal et al. [99], where 1 = no vascular browning; 2 = 1-25% of vascular browning; 3 = 26-50% of vascular browning; 4 = 51-75% of vascular browning; 5 = more than 75% of vascular browning.
Finally, the efficacy of each of the treatments was calculated using the formula described by Abbott [100] with some modifications Equation (3): where X represents the severity index of the pathogen control (Foph + ) and Y is the severity index of each treatment at the end of the experiment.

Stomatal Conductance and Leaf Water Potential
Stomatal conductance (g s ) and leaf water potential (Ψ wf ) were determined using a fully expanded leaf randomly taken from the upper or middle section of the plant's canopy. g s was estimated with a steady-state porometer (SC-1, Decagon Devices Inc., Pullman, WA, USA). Subsequently, Ψ wf was estimated using a Schollander pressure chamber (PMS, Model 615, OR) considering the same leaf used to determine g s on totally sunny days at 50 DAI between 9:00 and 12:00 h.

Growth Parameters
The different organs (leaves, stems, and roots) of each plant per treatment were gathered at 50 DAI to obtain their dry weight. Leaf area was determined using digital images in TIFF format (Tagged Image File Format) (D3300, Nikon, Thailand); the images were analyzed using a Java image processing program (Image J; National Institute of Mental Health, Bethesda, MD, USA). The leaf-area ratio was obtained using the ratio between leaf area and total dry weight (TDW) as an indicator of biomass partitioning. Finally, the relative tolerance index (RTI) was also estimated using the TDW and calculated with Equation (4) described by Roussos et al. [101].

RTI =
Total biomass of inoculated plants Total biomass of plants without inoculation × 100 (4)

Chlorophyll and Carotenoid Content
Leaves from the middle third of each treatment were used to take a 0.03 g sample at 50 DAI. Then, liquid nitrogen was used to macerate the leaves, which were then homogenized in 4 mL of 80% acetone. To remove particles, the samples were then centrifuged (Model 420101, Becton Dickinson Primary Care Diagnostics, MD, USA) at 5000 rpm for 10 min. We added acetone to the supernatant to complete a final volume of 6 mL. Finally, spectrophotometer readings (Spectronic BioMate 3 UV-vis Thermo, Madison, WI, USA) were performed at wavelengths of 663 and 646 nm for chlorophyll and 470 nm for carotenoids. The equations proposed by Lichtenthaler [102] were considered to determine the content of these pigments.

Malondialdehyde and Proline Content
The thiobarbituric acid method [103] was used to determine lipid oxidation (Malondialdehyde-MDA). At 50 DAI, 0.3 g of leaves from the upper or middle section of plants from each treatment were macerated and stored in liquid nitrogen. The samples were then centrifuged at 5000 rpm for 10 min and the absorbances were determined at 440, 532, and 600 nm using a spectrophotometer. MDA concentration was obtained using the extinction coefficient (157 M mL −1 ).
Leaf proline concentration was determined using the ninhydrin acid method [104]. Leaf samples of 0.3 g from leaves from the upper or middle section of the canopy of all treatments were also macerated in liquid nitrogen. Then, 10 mL of a 3% sulfosalicylic acid aqueous solution was added. Subsequently, samples were filtered using no. 2 Whatman paper; 2 mL of this filtrate was reacted with 2 mL of ninhydrin acid and 2 mL of glacial acetic acid. The mixture was left in a water bath at 90 • C for 1 h, stopping the reaction by incubation in ice. Four milliliters of toluene were used to dissolve the resulting solution which was shaken with a vortex shaker (V-1, BOECO, Hamburg, Germany) for 30 s. Finally, the absorbance was determined at 520 nm using a spectrophotometer. A standard calibration curve (Equation (5)) was used to determine the proline content with the fresh weight of the sample.

Experimental Design and Data Analysis
A factorial design was used for the data analysis, in which the first factor corresponded to the inoculation (with and without Foph) and the treatments used (Mix, MixRh, MixChi, and control) as a second factor. Each of the treatments consisted of eight plants per replicate. An analysis of variance (ANOVA) was performed, and a Tukey's post hoc test was used for comparison of means when significant differences (p ≤ 0.05) were found. A correlation analysis between RTI and AUDPC, vascular browning, Ψ wf, or proline was carried out to obtain the best treatments under inoculation conditions (Foph + ). Additionally, the comparison of the treatment effect on the evaluated variables was made taking as reference the response of the normalized absolute control. The arcsine function was used to transform percentage values. Statistix v 9.0 (Analytical Software, Tallahassee, FL, USA) was utilized to analyze data and SigmaPlot (version 12.0; Systat Software, San José, CA, USA) was used to make the figures, a three-dimensional graph, and perform the correlation analysis.

Conclusions
In summary, the results of this research indicated that the use of the different mixtures had a positive impact on cape gooseberry plants to mitigate vascular wilt. However, the mixtures T. virens + B. velezensis (Mix) and T. virens + B. velezensis with the addition of chitosan (MixChi) obtained the highest efficiencies in controlling the disease. Additionally, these treatments (Mix or MixChi) showed a biostimulant and growth-promoting effect, lower damage to membranes (low MDA contents), and pigment contents similar to those obtained in absolute control plants (Foph − ). These results suggest that the use of these mixtures in cape gooseberry plants could be considered as a complementary tool for the integrated management of the disease in areas where this crop is produced.