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Article

Effects of Vitamin D Deficiency on Proliferation and Autophagy of Ovarian and Liver Tissues in a Rat Model of Polycystic Ovary Syndrome

1
Department of Obstetrics and Gynecology, Semmelweis University, Üllői út 78/a, 1082 Budapest, Hungary
2
Department of Pharmacology and Pharmacotherapy, Semmelweis University, Nagyvárad tér 4, 1089 Budapest, Hungary
3
Department of Physiology, Semmelweis University, Tűzoltó utca 37-47, 1094 Budapest, Hungary
4
Institute of Clinical Experimental Research, Semmelweis University, Tűzoltó utca 37-47, 1094 Budapest, Hungary
*
Author to whom correspondence should be addressed.
These authors have contributed equally to the work.
Biomolecules 2019, 9(9), 471; https://doi.org/10.3390/biom9090471
Received: 31 July 2019 / Revised: 28 August 2019 / Accepted: 6 September 2019 / Published: 10 September 2019
(This article belongs to the Section Molecular Medicine)
Aim: We aimed to examine the alterations of the insulin signaling pathway, autophagy, nitrative stress and the effect of vitamin D supplementation in the liver and ovaries of vitamin D deficient hyperandrogenic rats. Methods: Female Wistar rats received eight weeks of transdermal testosterone treatment and lived on a low vitamin D diet (D–T+). Vitamin D supplementation was achieved by oral administration of vitamin D3 (D+T+). Sham-treated (D+T–) and vitamin D deficient animals (D–T–) served as controls. (N = 10–12 per group). Results: D–T+ animals showed decreased LC3 II levels in the liver and increased p-Akt/Akt and p-eNOS/eNOS ratios with decreased insulin receptor staining in the ovaries. Vitamin D supplementation prevented the increase of Akt phosphorylation in the ovaries. Vitamin D deficiency itself also led to decreased LC3 II levels in the liver and decreased insulin receptor staining in the ovaries. D–T+ group showed no increase in nitrotyrosine staining; however, the ovaries of D–T– rats and the liver of D+T+ animals showed increased staining intensity. Conclusion: Vitamin D deficiency itself might lead to disrupted ovarian maturation and autophagy malfunction in the liver. Preventing Akt phosphorylation may contribute to the beneficial effect of vitamin D treatment on ovarian function in hyperandrogenism. View Full-Text
Keywords: hyperandrogenism; vitamin D; polycystic ovary syndrome (PCOS); insulin resistance; autophagy; oxidative stress hyperandrogenism; vitamin D; polycystic ovary syndrome (PCOS); insulin resistance; autophagy; oxidative stress
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MDPI and ACS Style

Lajtai, K.; Nagy, C.T.; Tarszabó, R.; Benkő, R.; Hadjadj, L.; Sziva, R.E.; Gerszi, D.; Bányai, B.; Ferdinandy, P.; Nádasy, G.L.; Giricz, Z.; Horváth, E.M.; Várbíró, S. Effects of Vitamin D Deficiency on Proliferation and Autophagy of Ovarian and Liver Tissues in a Rat Model of Polycystic Ovary Syndrome. Biomolecules 2019, 9, 471. https://doi.org/10.3390/biom9090471

AMA Style

Lajtai K, Nagy CT, Tarszabó R, Benkő R, Hadjadj L, Sziva RE, Gerszi D, Bányai B, Ferdinandy P, Nádasy GL, Giricz Z, Horváth EM, Várbíró S. Effects of Vitamin D Deficiency on Proliferation and Autophagy of Ovarian and Liver Tissues in a Rat Model of Polycystic Ovary Syndrome. Biomolecules. 2019; 9(9):471. https://doi.org/10.3390/biom9090471

Chicago/Turabian Style

Lajtai, Krisztina, Csilla T. Nagy, Róbert Tarszabó, Rita Benkő, Leila Hadjadj, Réka E. Sziva, Dóra Gerszi, Bálint Bányai, Péter Ferdinandy, György L. Nádasy, Zoltán Giricz, Eszter M. Horváth, and Szabolcs Várbíró. 2019. "Effects of Vitamin D Deficiency on Proliferation and Autophagy of Ovarian and Liver Tissues in a Rat Model of Polycystic Ovary Syndrome" Biomolecules 9, no. 9: 471. https://doi.org/10.3390/biom9090471

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