Stimulation of the Production of Prostaglandin E2 by Ethyl Gallate, a Natural Phenolic Compound Richly Contained in Longan

Ethyl gallate is a phenolic compound richly contained in Longan. In traditional Chinese medicine, Longan is widely known as a fruit with “hot” properties, with a tendency to promote inflammatory and certain other responses. The mechanism for its proinflammatory as well as health beneficial effects is poorly understood. Based on our earlier observation that certain natural phenolic compounds can serve as reducing cosubstrates for cyclooxygenases (COXs), we sought to test a hypothesis that ethyl gallate may activate the catalytic activity of the COX enzymes. Results from studies using cultured cells and animals show that ethyl gallate can activate the production of prostaglandin E2, a representative prostaglandin tested in this study. Computational analysis indicates that ethyl gallate can activate the peroxidase active sites of COX-1 and COX-2 by serving as a reducing cosubstrate. The effect of ethyl gallate is abrogated by galangin, which is known to bind to the same peroxidase active sites of COX-1 and COX-2 as a competitive inhibitor. The findings of this study offer support for a novel hypothesis that the proinflammatory as well as health beneficial effects of Longan may be partly attributable to the activation of COX-1 and COX-2 by ethyl gallate.

Longan (also known as Dimocarpus longan) is a member of the soapberry family (Sapindaceae). It is grown extensively in China and South East Asia, as well as in Australia, Florida (United States of America (U.S.A.)), southern Europe, and southern Africa [8,9]. In traditional Chinese medicine, Longan is widely known as a fruit with "hot" properties (i.e., it has a tendency to promote inflammatory and certain other responses) [10][11][12][13]. Studies have shown that Longan contains high levels of ethyl gallate [14,15] and other phenolic compounds [15][16][17]. Ethyl gallate is also found in other plants, such as walnuts [18] and Terminalia myriocarpa [19], and it is also present in wine [20].
The mechanism underlying the proinflammatory and health beneficial effects of Longan is poorly understood at present. In earlier studies [21,22], we showed, for the first time, that some natural phenolics, such as quercetin and myricetin, can serve as reducing cosubstrates of COX-1 and COX-2

In Vitro Cell Culture Experiments
The RAW264.7 cell line (murine macrophages) was purchased from Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (CAS), and maintained in DMEM containing L-glutamine, glucose, and sodium bicarbonate supplemented with 10% fetal bovine serum at 37 • C under 5% CO 2 . As described in our earlier studies [21,24], the cells were first treated with LPS (at 1 µg/mL) for 2 h to increase the expression levels of the COX enzymes (almost exclusively COX-2). Then, the culture medium was removed and replaced with 300 µL of serum-free medium in the absence or presence of different concentrations of ethyl gallate. After culturing for additional 2 h, the medium was collected for measurement of PGE 2 using an EIA kit (Cayman Chemical) according to the detailed procedures provided by the manufacturers.

In Vivo Animal Experiments
All procedures involving the use of live animals as described in the present study were approved by the Institutional Animal Care and Use Committee (approval number: SUSTC-G-2014009), and the guidelines for humane treatment of animals accepted by the National Institutes of Health (U.S.A.) were followed. The male Sprague-Dawley rats (four to five-week-old, specific pathogen-free) were obtained from Guangdong Medical Laboratory Animal Center (Guangdong, China) and were maintained in our institute's central animal facility. The animals were fed a standard rodent chow purchased from Beijing Keaoxieli (Beijing, China). After arrival, the animals were allowed to acclimatize for one week prior to being used in the experimentation. The animals were housed under constant conditions of temperature (20 ± 1 • C) and 12-h light/12-h dark cycle and had free access to food and water.
The male rats were divided into the following groups (five animals per group): the control group (treated with the vehicle only) and the ethyl gallate treatment group (receiving ethyl gallate at 6 mg/kg body weight, dissolved in 1.5 mL of 1% methyl cellulose). Ethyl gallate was given via gastric intubation, and the control animals were given 1.5 mL of vehicle only. The use of the 6 mg/kg body weight dose of ethyl gallate was based on an initial dose-escalating experiment, which showed that this in vivo oral dose causes a consistent and reproducible increase in PGE 2 plasma levels in rats. Whole blood samples were collected through tail bleeding at different timepoints following ethyl gallate or vehicle administration and stored in small vials containing heparin. Plasma was prepared from the collected blood by centrifugation. The plasma level of PGE 2 was determined using an EIA kit (Cayman Chemical) according to the manufacturer's instructions.

Molecular Docking Analysis of the Binding of Ethyl Gallate with COX Enzymes
In this study, a Dell PowerEdge R730 Server with the Discovery Studio modeling software (Version 2007; Accelrys, San Diego, CA, USA) was used for various computational analyses, as described below.

Protein Processing
We used the X-ray structures of sheep COX-1 (PDB code: 1q4g [25]) and mouse COX-2 (PDB code: 3nt1 [4]) as templates for docking analysis. It is of note that in these two COX structures, protoporphorin IX with Fe IV inside (P + Fe IV ) is already present as an integral component, and the ion atom is set as Fe 4+ . As part of the protein preparation step, all small molecules except P + Fe IV that are non-covalently attached to the COX proteins were removed, and then, the amino acid residues in the protein structure were re-numbered according to the correct known sequences. The Clean Protein module in Discovery Studio was used to complete the side chains for the amino acid residues, correct bonding and bond orders, and add hydrogens back. Lastly, the Prepare Protein module in Discovery Studio was used for protein preparation under the CHARMm force field setting.

Ligand Processing
The structure of ethyl gallate was downloaded from the Protein Data Bank and minimized with the CHARMm force field. In addition, we used the Prepare Ligands module to generate ethyl gallate in a non-ionizing state and two partially-ionizing states. The non-ionizing state has all hydrogens in the three phenolic hydroxyl groups retained, whereas the ionizing states each have one proton removed (i.e., deprotonation) from one of the three hydroxyl groups in ethyl gallate, which include C-3-OH (equivalent to C-5-OH) and C-4-OH (see Figure 1). from the collected blood by centrifugation. The plasma level of PGE2 was determined using an EIA kit (Cayman Chemical) according to the manufacturer's instructions.

Molecular Docking Analysis of the Binding of Ethyl Gallate with COX Enzymes
In this study, a Dell PowerEdge R730 Server with the Discovery Studio modeling software (Version 2007; Accelrys, San Diego, CA, USA) was used for various computational analyses, as described below.

Protein Processing
We used the X-ray structures of sheep COX-1 (PDB code: 1q4g [25]) and mouse COX-2 (PDB code: 3nt1 [4]) as templates for docking analysis. It is of note that in these two COX structures, protoporphorin IX with Fe IV inside (P + Fe IV ) is already present as an integral component, and the ion atom is set as Fe 4+ . As part of the protein preparation step, all small molecules except P + Fe IV that are non-covalently attached to the COX proteins were removed, and then, the amino acid residues in the protein structure were re-numbered according to the correct known sequences. The Clean Protein module in Discovery Studio was used to complete the side chains for the amino acid residues, correct bonding and bond orders, and add hydrogens back. Lastly, the Prepare Protein module in Discovery Studio was used for protein preparation under the CHARMm force field setting.

Ligand Processing
The structure of ethyl gallate was downloaded from the Protein Data Bank and minimized with the CHARMm force field. In addition, we used the Prepare Ligands module to generate ethyl gallate in a non-ionizing state and two partially-ionizing states. The non-ionizing state has all hydrogens in the three phenolic hydroxyl groups retained, whereas the ionizing states each have one proton removed (i.e., deprotonation) from one of the three hydroxyl groups in ethyl gallate, which include C-3-OH (equivalent to C-5-OH) and C-4-OH (see Figure 1).

Figure 1.
Chemical structure of ethyl gallate, a natural phenolic compound. For instance, after ionization (deprotonation) of the C-4-OH hydroxyl group, its oxygen atom in this hydroxyl group carries a negative charge with an additional electron retained. Similar ionization can also occur with the C-3-OH group, as well as the C-5-OH group.

Flexible Docking
For flexible docking, we used the Find Sites from Receptor Cavities module to identify the binding site in the prepared 1q4g COX-1 and 3nt1 COX-2 structures. According to our earlier study, the target Figure 1. Chemical structure of ethyl gallate, a natural phenolic compound. For instance, after ionization (deprotonation) of the C-4-OH hydroxyl group, its oxygen atom in this hydroxyl group carries a negative charge with an additional electron retained. Similar ionization can also occur with the C-3-OH group, as well as the C-5-OH group.

Flexible Docking
For flexible docking, we used the Find Sites from Receptor Cavities module to identify the binding site in the prepared 1q4g COX-1 and 3nt1 COX-2 structures. According to our earlier study, the target site is the peroxidase active site in these two COX proteins [22]. We selected all amino acid residues within a 5 Å reach of the target site and allowed them to have flexible side chains. The SBD Site Sphere is centered at the target site and then expanded to 13-radius size. Under the Flexible Docking mode, with the conformation method set to BEST, the Simulated Annealing docking method was then applied to dock ethyl gallate into the target sites of COX-1 and COX-2. Notably, two flexible docking modes were separately executed for COX-1 and COX-2, corresponding to the two different ionizing states of ethyl gallate. The whole structure of each COX protein was further minimized with the CHARMm force field.

Calculation of Binding Energy
Following the completion of the flexible docking procedure, the Calculate Binding Energies module in Discovery Studio was used to find the complexes with the lowest binding energy values. According to Discovery Studio, the free energy for the binding interaction between a protein and its ligand is estimated from the free energies of the complex, the protein, and the ligand. These free energy values were separately calculated using the CHARMm force field and the Poisson-Boltzmann equation with non-polar surface area (PBSA) method [26]. In this approach, the Poisson-Boltzmann equation is solved numerically on a three-dimensional (3D) grip, and the calculated electrostatic potential is used to estimate the electrostatic solvation free energy. The ligand conformational entropy is also considered during the free binding energy calculation. The following equation is used to calculate the binding energy (∆G binding ) between ethyl gallate and the COX-1 or COX-2 protein: where G complex is the absolute free energy of the complex, G COX is the absolute free energy of the COX protein, and G ligand is the absolute free energy of the ligand [27,28]. The ∆G binding value is used to reflect the relative interaction affinity between the COX enzyme and ethyl gallate. The free energy of each term is estimated as a sum of the following five terms: where <G intra > is the intramolecular energy of the molecule, <G inter > is the intermolecular energy of the molecule, <G pol > is the polar contribution to solvation free energy, <G np > is the nonpolar contribution to solvation free energy, and (-T∆S) is the entropic contribution (set at 298.15 K).

In Vitro Studies
As described in our recent studies [21,24], to determine whether ethyl gallate can modulate PG production in cultured RAW264.7 cells, these cells need to be first stimulated with 1 µg/mL of LPS for cosubstrates [21]. For comparison, we also tested 5,4'-dihydroxyflavone and 7,4'-dihydroxyflavone (two analogs of quercetin) for their effect on PGE 2 production in this cell culture model as a positive comparison. We found that these two compounds stimulate the production of PGE 2 in a similar manner as ethyl gallate but with a somewhat weaker efficacy ( Figure 2, middle and right).  In this study, we showed that galangin can abrogate the stimulation of PGE2 production by ethyl gallate in a concentration-dependent manner, with an IC50 value (50% inhibition concentration) of approximately 0.4 µM (Figure 3, left). When galangin was added alone to the LPS-pretreated RAW264.7 cells in the culture, it also inhibited the baseline production of PGE2 in a similar manner with an IC50 value of approximately 0.7 µM (Figure 3, right). It is of note that similar observations were also made in our recent study [29].  Effect of ethyl gallate, 5,4 -dihydroxyflavone, and 7,4 -dihydroxyflavone on prostaglandin E 2 (PGE 2 ) release from lipopolysaccharide (LPS)-pretreated RAW264.7 cells. Cells were pretreated with 1 µg/mL of LPS for 2 h to induce Cyclooxygenase 2 (COX-2) expression, and then, the culture media were removed and replaced with 300 µL of serum-free medium containing the test compound ethyl gallate for another 2 h. The following concentrations of ethyl gallate were used: 0.01, 0.1, 1, and 10 µM. The levels of PGE 2 were measured using an enzymatic immunoassay (EIA) kit (Cayman Chemical). The PGE 2 level in the control group is 1.5 ng/mL. Each point was the mean ± standard deviation (SD) of triplet determinations.
In this study, we showed that galangin can abrogate the stimulation of PGE 2 production by ethyl gallate in a concentration-dependent manner, with an IC 50 value (50% inhibition concentration) of approximately 0.4 µM (Figure 3, left). When galangin was added alone to the LPS-pretreated RAW264.7 cells in the culture, it also inhibited the baseline production of PGE 2 in a similar manner with an IC 50 value of approximately 0.7 µM (Figure 3, right). It is of note that similar observations were also made in our recent study [29].  In this study, we showed that galangin can abrogate the stimulation of PGE2 production by ethyl gallate in a concentration-dependent manner, with an IC50 value (50% inhibition concentration) of approximately 0.4 µM (Figure 3, left). When galangin was added alone to the LPS-pretreated RAW264.7 cells in the culture, it also inhibited the baseline production of PGE2 in a similar manner with an IC50 value of approximately 0.7 µM (Figure 3, right). It is of note that similar observations were also made in our recent study [29].

In Vivo Studies
We also determined the effect of ethyl gallate on the plasma levels of PGE 2 by using normal male Sprague-Dawley rats as an in vivo model. This animal model was chosen for this purpose, because we have successfully used it in similar studies to determine the effect of other natural compounds on plasma and tissue levels of several PG products [21,24]. We showed earlier that oral administration of these natural compounds to normal rats can effectively increase the tissue and/or blood levels of PG products in vivo [21,24].
In this experiment, the animals received a single oral dose of ethyl gallate alone (at 6 mg/kg body weight). Blood samples were collected through tail bleeding at selected timepoints, and the plasma samples were prepared and used for PGE 2 measurement. We find that oral administration of ethyl gallate alone markedly increases the plasma level of PGE 2 in a time-dependent manner (Figure 4). Plasma PGE 2 level increased significantly at 3 h after administration, and peaks at approximately 6 h after administration, with a maximal increase by approximately four-fold of the control level ( Figure 4). culture media were removed and replaced with 300 µL of serum-free medium containing 1 µM ethyl gallate plus different concentrations (0.01, 0.1, 1, and 10 µM) of galangin for another 2 h. The levels of PGE2 were measured using an EIA kit (Cayman Chemical). The PGE2 level in the control group is 1.5 ng/mL. Each point was the mean ± SD of triplet determinations.

In Vivo Studies
We also determined the effect of ethyl gallate on the plasma levels of PGE2 by using normal male Sprague-Dawley rats as an in vivo model. This animal model was chosen for this purpose, because we have successfully used it in similar studies to determine the effect of other natural compounds on plasma and tissue levels of several PG products [21,24]. We showed earlier that oral administration of these natural compounds to normal rats can effectively increase the tissue and/or blood levels of PG products in vivo [21,24].
In this experiment, the animals received a single oral dose of ethyl gallate alone (at 6 mg/kg body weight). Blood samples were collected through tail bleeding at selected timepoints, and the plasma samples were prepared and used for PGE2 measurement. We find that oral administration of ethyl gallate alone markedly increases the plasma level of PGE2 in a time-dependent manner ( Figure 4). Plasma PGE2 level increased significantly at 3 h after administration, and peaks at approximately 6 h after administration, with a maximal increase by approximately four-fold of the control level ( Figure  4). In summary, in vitro experiments using cultured cells and in vivo experiments using normal male rats as an animal model both show that ethyl gallate can stimulate the production of PGE2. This effect is abrogated by galangin, which is an inhibitor of the COX peroxidase activity by blocking the effect of the reducing cosubstrate. In summary, in vitro experiments using cultured cells and in vivo experiments using normal male rats as an animal model both show that ethyl gallate can stimulate the production of PGE 2 . This effect is abrogated by galangin, which is an inhibitor of the COX peroxidase activity by blocking the effect of the reducing cosubstrate.

Computational Docking Analysis of Ethyl Gallate Binding Inside the Peroxidase Active Sites of COX-1 and COX-2
We used sheep COX-1 (PDB code: 1q4g [25]) and mouse COX-2 (PDB code: 3nt1 [4]) proteins as templates to model how ethyl gallate binds inside the peroxidase active sites of these two enzymes. Three-dimensional (3D) structural models of these two proteins were prepared by Discovery Studio. Using these structural models, we then docked ethyl gallate in three different ionizing states (one non-ionizing state vs two partially-ionizing states) into the peroxidase active sites of COX-1 and COX-2. The results are described below.

COX-1
Computational docking analysis of ethyl gallate in a non-ionizing state suggests that it can bind inside the peroxidase active site of COX-1 in two possible binding modes: one with its A-ring inside the peroxidase site facing P + Fe IV , and the other one with its side chain structure inside the peroxidase site. Based on the binding energy ∆G binding values (Table 1), it is predicted that the binding mode with its A-ring inside is the dominant binding pose (∆G binding of −3.652 k cal /mol). However, in this binding pose, most hydroxyl groups of ethyl gallate are not too close to the Fe ion of P + Fe IV , and the closest one is 8.762 Å ( Figure 5A,B). These results suggest that this binding pose is an inactive pose and would not be able to effectively transfer its electrons to P + Fe IV for reduction. For poses ranked 2-10, all hydroxyl groups of ethyl gallate are slightly farther away from the Fe ion of P + Fe IV compared with the dominant binding pose.
Potential hydrogen bonds between COX-1 and ethyl gallate in its dominant binding pose, as suggested by the Receptor-Ligand Hydrogen Bonds module, are shown in Figure 5C, which involve two amino acid residues: one with Phe210 (1.883 Å) and one with His207 (1.335 Å).
It is predicted that under physiological conditions, a small fraction of the hydroxyl groups in ethyl gallate's A-ring would undergo ionization (deprotonation), i.e., removal of a proton. Results from our recent study [29] suggest that the binding interaction of quercetin (a reducing cosubstrate) under partial ionization is dramatically enhanced in comparison with its non-ionizing state. Based on this interesting earlier finding, we also perform docking analysis using the partially-ionizing ethyl gallate. Predicted by Discovery Studio, C-3-OH of ethyl gallate has a slightly higher tendency to deprotonate than C-4-OH under physiological conditions. In the present study, we chose to determine the docking conformation when deprotonation occurs with only one hydroxyl group at any given moment, because simultaneous deprotonation of multiple protons in the same molecule are considered a rare occurrence under physiological pH conditions. We find that when each of the phenolic hydroxyl groups is individually deprotonated, the dominant poses (based on ∆G binding values; Table 1) all have their ring structures inside ( Figure 5D, 5G). Under C-3-OH (or C-5-OH) deprotonation ( Figure 5H; ∆G binding of −108.779 k cal /mol), the distance between the Fe ion and Ois 2.254 Å. Under C-4-OH deprotonation ( Figure 5E; ∆G binding of -94.444 k cal /mol), the distance between Fe and Ois 2.373 Å. This data suggests that both C-3-OH and C-4-OH can donate their electron to the Fe ion of P + Fe IV .
Potential hydrogen bonds between COX-1 and ethyl gallate in two ionizing states, as suggested by the Receptor-Ligand Hydrogen Bonds, are shown in Figure 5F and 5I. Under C-3-OH deprotonation, ethyl gallate in its dominant binding pose may form one hydrogen bond with His207 (2.420 Å); under C-4-OH deprotonation, it may form three hydrogen bonds: two with His207 (1.284 Å and 2.378 Å) and one with Gln203 (2.093 Å). Table 1. Computed binding energy values (∆G binding , k cal /mol) for the molecular docking analysis of the best binding poses between ethyl gallate (partially-ionized vs non-ionized) and cyclooxygenase 1 and 2 (COX-1 and COX-2) proteins.

Type of Protein
Binding Energy Value ∆G binding (k cal /mol)

COX-2
Similar computational docking analysis was also performed with the COX-2 protein. Based on the calculated binding energy ∆G binding values, it was predicted that the dominant binding pose of ethyl gallate in a non-ionizing state has its side chain structure inside the peroxidase site of COX-2 (∆G binding of −6.398 k cal /mol; Figure 6A). All hydroxyl groups in this pose are not within the catalytically-effective distance (the shortest distance from the Fe ion of P + Fe IV is 8.762 Å; Figure 6B). For poses ranked 2-10, the hydroxyl groups of ethyl gallate are slightly farther away from the Fe ion of P + Fe IV compared with the dominant binding pose.
We also analyzed the docking conformations when deprotonation occurs with ethyl gallate's phenolic hydroxyl groups ( Figure 6D,E). We found that when C-4-OH is deprotonated, the dominant pose has its ring structure closer to the Fe ion of P + Fe IV (∆G binding of −100.929 k cal /mol; Figure 6G). In addition, the Fe ion of P + Fe IV is very close to the Oion in ethyl gallate's C-4-OH (2.332 Å). Under C-3-OH deprotonation, the dominant pose ( Figure 6H) also has its ring inside (∆G binding of −132.742 k cal /mol), with a distance of 2.348 Å between the Fe ion and Oion. The suggested potential hydrogen bonds in the dominant poses in three different ionization states are shown in Figure 6C,F,I.

Discussion
In this study, we demonstrate that ethyl gallate can activate PGE 2 production both in vitro and in vivo. It is suggested that ethyl gallate exerts this effect through the activation of the peroxidase active site of COX-1 and COX-2 by serving as a reducing cosubstrate for P + Fe IV , in a similar manner as quercetin (reported in [21][22][23][24]). This suggestion is based on the following observations: (i) The activating effect of ethyl gallate on PGE 2 production in vitro and in vivo reported in this study closely mirrors the activating effect of quercetin reported earlier [21,23]. (ii) Activation of PGE 2 production by ethyl gallate can be inhibited in a concentration-dependent manner by galangin, and a mirroring inhibitory effect of galangin was also observed recently with quercetin [29]. (iii) Computational docking analysis indicates that ethyl gallate can bind inside the peroxidase active sites of COX-1 and COX-2 in similar manners as quercetin, which presumably would result in re-activation of the peroxidase active sites. Moreover, our recent computational docking analysis [29] reveals that galangin can also bind inside the same peroxidase active sites of COX-1 and COX-2 in an inhibitor mode [29]. Together, these lines of data provide support for the suggestion that ethyl gallate shares a similar mechanism of action on COX-1 and COX-2 as quercetin.
Computational analysis provides useful insights into the possible mechanism by which ethyl gallate activates COX-1 and COX-2 at the molecular level. In the case of COX-1, comparison of ethyl gallate in both non-ionizing and partially-ionizing states indicates that ionization of C-3-OH or C-4-OH markedly shortens the distance between Fe 4+ and the respective O -(from 5.129 Å to 2.254 or 2.373 Å, respectively) and increases the binding affinity (∆G binding value decreases from −3.652 k cal /mol to −108.779 or −94.444 k cal /mol, respectively). Similarly, in the case of COX-2, ethyl gallate in the C-3-OH or C-4-OH partially-ionizing state also markedly shortens the distance between Fe 4+ and the respective O -(from 8.762 Å to 2.348 or 2.332 Å, respectively) and increases the binding affinity (∆G binding value decreases from −6.398 k cal /mol to −132.742 or −100.929 k cal /mol, respectively).
While C-3-OH and C-4-OH of ethyl gallate can both contribute an electron for the reduction of the COX-1 and COX-2 enzymes, differences are also noted in these two isozymes. In the case of COX-1, C-3-OH deprotonation causes the chemical to move closer to the Fe ion of P + Fe IV than C-4-OH deprotonation. However, in the case of COX-2, C-4-OH deprotonation causes the chemical to move closer to the Fe ion of P + Fe IV than C-3-OH deprotonation.
It is worth noting that our earlier studies [21][22][23] showed that quercetin, myricetin, and other reducing cosubstrates of the COX enzymes share a dual stimulation-inhibition phenomenon. At low concentrations, these compounds stimulate the COX enzymes as reducing cosubstrates, but at very high concentrations, they start to inhibit the catalytic activity of the COX enzymes. As explained in our earlier study [22], the main reason is because PGG 2 (the substrate) and the reducing cosubstrate occupy the same peroxidase site. The peroxidase site needs to have the reducing cosubstrate for its re-activation after each catalytic cycle, but when the concentration of a reducing cosubstrate is too high, it would limit the access of PGG 2 to the same site, thereby posing a net inhibitory effect on the overall catalytic activity and PG formation. Similarly, results from our present study show that ethyl gallate, 5,4 -dihydroxyflavone, and 7,4 -dihydroxyflavone also have a dual stimulation-inhibition effect. At low concentrations, they stimulate PGE 2 production, but their effect is diminished at high concentrations or even becomes inhibitory.
In traditional Chinese medicine, Longan is known as a fruit with "hot" properties, i.e., it has the tendency to promote inflammatory and certain other responses. However, the mechanism of its proinflammatory as well as health beneficial effects is poorly understood. The results of our present study show that ethyl gallate, a natural phenolic compound richly contained in Longan, can stimulate PG production, likely through the activation of the catalytic activity of the COX-1 and COX-2 enzymes by serving as a reducing cosubstrate for these enzymes. Similarly, a recent study from our laboratory [24] reported that ellagic acid, another phenolic compound contained in Longan and its related fruit Litchi [14][15][16][30][31][32][33], also shares this pharmacological property. It is plausible that the unique stimulatory effect of ethyl gallate and ellagic acid on PG production may partly contribute to the proinflammatory as well as health beneficial effects of Longan and Litchi.
In summary, the results of our present study show that ethyl gallate is an activator of PGE 2 production in vitro and in vivo. Mechanistically, it is suggested that ethyl gallate exerts this effect through the activation of the peroxidase active site of COX-1 and COX-2 by serving as a reducing cosubstrate for the Fe ion of P + Fe IV . The effect of ethyl gallate is similar to the effect of some other naturally-occurring phenolics that were reported earlier [21][22][23][24].