Next Article in Journal / Special Issue
Pyranose Dehydrogenase from Agaricus campestris and Agaricus xanthoderma: Characterization and Applications in Carbohydrate Conversions
Previous Article in Journal
The Impact of Sphingosine Kinase-1 in Head and Neck Cancer
Previous Article in Special Issue
Enzyme-Catalyzed Synthesis of Unsaturated Aliphatic Polyesters Based on Green Monomers from Renewable Resources
Article Menu

Export Article

Open AccessArticle
Biomolecules 2013, 3(3), 514-534;

Lipases Immobilization for Effective Synthesis of Biodiesel Starting from Coffee Waste Oils

Dipartimento di Scienze Chimiche e Farmaceutiche, Università degli Studi di Trieste, Piazzale Europa 1, Trieste 34127, Italy
Department of Chemical Engineering, Faculty of Engineering, University of Malaya, Malaysia
Flavia 110, Trieste 34147, Italy
Author to whom correspondence should be addressed.
Received: 28 June 2013 / Revised: 4 August 2013 / Accepted: 6 August 2013 / Published: 13 August 2013
(This article belongs to the Special Issue Enzymes and Their Biotechnological Applications)
Full-Text   |   PDF [860 KB, uploaded 13 August 2013]   |  


Immobilized lipases were applied to the enzymatic conversion of oils from spent coffee ground into biodiesel. Two lipases were selected for the study because of their conformational behavior analysed by Molecular Dynamics (MD) simulations taking into account that immobilization conditions affect conformational behavior of the lipases and ultimately, their efficiency upon immobilization. The enzymatic synthesis of biodiesel was initially carried out on a model substrate (triolein) in order to select the most promising immobilized biocatalysts. The results indicate that oils can be converted quantitatively within hours. The role of the nature of the immobilization support emerged as a key factor affecting reaction rate, most probably because of partition and mass transfer barriers occurring with hydrophilic solid supports. Finally, oil from spent coffee ground was transformed into biodiesel with yields ranging from 55% to 72%. The synthesis is of particular interest in the perspective of developing sustainable processes for the production of bio-fuels from food wastes and renewable materials. The enzymatic synthesis of biodiesel is carried out under mild conditions, with stoichiometric amounts of substrates (oil and methanol) and the removal of free fatty acids is not required. View Full-Text
Keywords: lipases; immobilization; biodiesel; oil from spent coffee ground lipases; immobilization; biodiesel; oil from spent coffee ground

Figure 1

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Supplementary material


Share & Cite This Article

MDPI and ACS Style

Ferrario, V.; Veny, H.; De Angelis, E.; Navarini, L.; Ebert, C.; Gardossi, L. Lipases Immobilization for Effective Synthesis of Biodiesel Starting from Coffee Waste Oils. Biomolecules 2013, 3, 514-534.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Biomolecules EISSN 2218-273X Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top