Characterization of Triacylglycerol Estolide Isomers Using High-Resolution Tandem Mass Spectrometry with Nanoelectrospray Ionization

Triacylglycerol estolides (TG-EST) are biologically active lipids extensively studied for their anti-inflammatory and anti-diabetic properties. In this work, eight standards of TG-EST were synthesized and systematically investigated by nanoelectrospray tandem mass spectrometry. Mass spectra of synthetic TG-EST were studied with the purpose of enabling the unambiguous identification of these lipids in biological samples. TG-EST glycerol sn-regioisomers and isomers with the fatty acid ester of hydroxy fatty acid (FAHFA) subunit branched in the ω-, α-, or 10-position were used. Ammonium, lithium, and sodium adducts of TG-EST formed by nanoelectrospray ionization were subjected to collision-induced dissociation (CID) and higher-energy collisional dissociation (HCD). Product ion spectra allowed for identification of fatty acid (FA) and FAHFA subunits originally linked to the glycerol backbone and distinguished the α-branching site of the FAHFA from other estolide-branching isomers. The ω- and 10-branching sites were determined by combining CID with ozone-induced dissociation (OzID). Lithium adducts provided the most informative product ions, enabling characterization of FA, hydroxy fatty acid (HFA), and FAHFA subunits. Glycerol sn-regioisomers were distinguished based on the relative abundance of product ions and unambiguously identified using CID/OzID of lithium and sodium adducts.


TG-EST Standards
The structures of eight TG-EST standards investigated in this work are shown in Figure 1.

TG-EST Standards
The structures of eight TG-EST standards investigated in this work are shown in Fig   The standards each had one FAHFA subunit in either the sn-1/3 (Standards TG-EST 1-7) or the sn-2 position (Standard TG-EST 8). The FAHFA subunits contained the estolide ester bond in positions-2 (α; Standards TG-EST 2, and 5), -10 (Standard TG-EST 3), or at the HFA chain terminus (ω; Standards TG-EST 1, 4, 6, 7, and 8). The standards were synthesized by Steglich esterification mediated by N,N -dicyclohexylcarbodiimide (DCC) [53]. 10-Hydroxyhexadecanoate, the key compound for preparation of TG-EST 3, was prepared according to our previous work [54]. Details regarding the synthesis, together with spectral data of intermediates and final products, are provided in Supplementary Materials (Schemes S1-S8 and Figures S1-S75).
For CID and HCD mass spectrometry experiments, the TG-EST standards were dissolved in a mixture of chloroform: propan-2-ol: acetonitrile: aqueous salt solution (0.5:0.05:0.80:0.10, by vol.) at a concentration of 10 µg mL −1 . The aqueous solutions of the salts (ammonium, lithium, or sodium formate) were prepared at a concentration of 1.0 mmol L −1 .

Mass Spectrometry
For CID and HCD workflows, nanoESI-MS n experiments were performed on the Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA) equipped with a robotic chip-based nanoESI apparatus TriVersa NanoMate (Advion, Inc., Ithaca, NY, USA). Data were recorded and interpreted manually using Xcalibur 4.1.50 software (Thermo). The TriVersa NanoMate, controlled by Chipsoft 8.3.1 software, was operated with an ionization spray voltage of 1800 V and nitrogen delivery gas at 0.5 psi. TG-EST solutions were loaded into 96-well plates, and the sample aliquots (5-15 µL) were aspirated and infused into the mass spectrometer. The Orbitrap mass analyzer was operated at a resolution of 120,000 FWHM, and m/z values were acquired with an accuracy of less than 3.0 ppm.
[M + NH 4 ] + , [M + Li] + , and [M + Na] + ions were fragmented in MS 2 and MS 3 using CID and HCD. Helium and nitrogen served as collision gases for CID and HCD, respectively. Values of normalized collision energies (NCEs) are shown in spectra and are a unitless parameter (as NCE automatically compensates for the mass dependence on collision energy). The precursor isolation window was 1.0-1.5 mass units. The mass spectra were recorded in the m/z range of 200−1200 for full MS and MS 2 , and a m/z range of 150-900 for MS 3 was used. The energy-resolved dissociation curves (Supplementary Materials, Figures S76-S87) were calculated from spectra acquired by introducing incremental changes to NCE values. The relative ion abundance values were averaged from 20-40 scans.
For MS 3 CID/OzID and MS 4 CID/CID/OzID sequential workflows, nanoESI-MS n experiments were performed on the LTQ Orbitrap Elite mass spectrometer (Thermo) modified for OzID experiments [55]. An ozone generator (Titan30 UHC; Absolute Ozone, Edmonton, AB, Canada) was used for the external production of ozone (ca. 17% in oxygen), which was introduced into the ion-trap region of the instrument. The TriVersa NanoMate was used in the same way as in the case of CID and HCD with identical parameters (ionization spray voltage, nitrogen delivery, resolution, precursor window, and accuracy). Lithiated estolide-branching isomers and lithiated/sodiated glycerol sn-regioisomers were activated by CID (NCE 40%). Depending on the sequential workflow, the re-isolated MS 2 or MS 3 CID product ions reacted with ozone for 1.0 s at NCE of 0-1%.

Mass Spectra of ω-, 10-, and α-Estolide-Branching Regioisomers
The FAHFA subunit of TG-EST consists of an HFA esterified (via its hydroxy group) to a second FA. The position of the hydroxy group can range from second (α-)carbon (adjacent to the carboxylic acid moiety) to the final (ω-)carbon on the chain terminus. We investigated ω-, 10-, and α-estolide-branching regioisomers to establish whether their mass spectra provided information characteristic of the estolide ester bond position. When subjected to positive ion electrospray ionization from solutions containing ammonium, lithium, and sodium cations, TG-EST produced abundant [M + NH 4 ] + , [M + Li] + , and [M + Na] + adducts, respectively.

Fragmentation of Ammonium Adducts
Ammonium formate and acetate are relatively volatile salts that are widely used in ESI and HPLC/MS. Ammonium adducts have been broadly adopted for the analysis neutral lipids, e.g., TGs [56,57], and they have also been utilized in the structural analysis of TG-ESTs [6][7][8][9][10][11]25,37]. Activation of these adducts typically leads to the neutral loss of ammonia, yielding product ions arising from proton transfer to the neutral lipid. MS 2 HCD spectra of TG-EST ammonium adducts yielded fragments consistent with the neutral loss of ammonia combined with FA and FAHFA. As illustrated in Figure 2,  . In this isomer (10-), eliminations of FA linked to glycerol and bound in the FAHFA moiety were similarly efficient. Neutral loss of FAs from FAHFA moiety was documented previously for TG-EST with estolide-branching site on "inner" carbons of HFA chain [6,7].   Figure S90D-F) showed less significant differences in peak intensities, making these fragmentation patterns less likely to be useful for discriminating between isomers in a complex biological mixture. In theory, further information about the estolide structure could also be obtained by re-isolation and further In addition to neutral loss peaks, FAHFA acylium ion (m/z 519. 48 [58] (p. 109). The abundant acylium ion made it possible to identify FA in the FAHFA subunit. As the acylium ions were formed only from the TG-EST 2 ( Figure 2B) and TG-EST 5 ( Figure S88B), they can be considered diagnostic ions for the α-estolide-branching regioisomers. Mass spectra MS 2 HCD of ammonium adducts of TG-EST 4-8 are presented in Supplementary Materials ( Figure S88).
The ion trap CID spectra contained fragments identical to HCD spectra, but the low mass cut-off in the ion trap prevented detection of product ions of m/z < 350 ( Figure S89). Therefore, HCD was more useful for TG-EST structure elucidation than CID. Energyresolved dissociation curves of TG-EST ammonium adducts helped us to determine the optimal value of NCE for MS 2 experiments. As shown in Figures S76-S79, the optimum NCEs in CID were almost the same for TG-EST 1-3 . In HCD, the optimum NCE values ensuring the detection of all diagnostic product ions were dependent on the TG-EST structure. While TG-EST 1 required NCE values above 20, TG-EST 2 fragmented best at NCE around 15, and TG-EST 3 needed an NCE value set to approximately 10. This observation is consistent with differences in the energetics of competing dissociation pathways across the isomeric structures.  Figure S90D-F) showed less significant differences in peak intensities, making these fragmentation patterns less likely to be useful for discriminating between isomers in a complex biological mixture. In theory, further information about the estolide structure could also be obtained by re-isolation and further dissociation of FAHFA acylium ion at m/z 519.48, but in practice, its abundance was too low to get measurable diagnostic product ion signals.
The MS 2 HCD spectra of TG-EST ammonium adducts made it possible to characterize FAHFA subunit and FAs linked to glycerol. FA bound in the FAHFA subunit was detectable as an acylium ion in α-isomer and as a neutral loss in the 10-isomer. Therefore, the spectra allowed us to distinguish estolide-branching isomers and characterize all four FA chains in TG-EST, except for ω-isomers, for which the spectra did not provide information about individual chains in the FAHFA moiety (see spectra of TG-EST 4 and TG-EST 6 in Figure S88A,C, respectively). Determining the estolide-branching position in MS 2 was thus possible for αand 10-isomers. MS 3 allowed us to obtain additional structural information; characteristic fragments indicating αand ω-isomers were identified.

Fragmentation of Lithium Adducts
Under CID and HCD conditions, lithium adducts of neutral lipids most often provide more structural information than the corresponding sodium or ammonium adducts due to the high oxygen affinity of the lithium [59][60][61]. Figure 3 shows MS 2 HCD spectra of lithiated lithiated TG-EST 1-3 (m/z 1089.96; C69H126O8Li + ). Some of the fragmentation pathways observed in these spectra resemble those observed for the ammonium adducts. Lithiated TG-EST eliminated palmitoleic acids linked to the glycerol backbone (m/z 835.74; C53H96O6Li + ) and the FAHFA moiety both as the neutral acid (m/z 553.48; C35H62O4Li + ) and its lithium salt (m/z 547.47; C35H63O4 + ). Eliminating oleic acid from the FAHFA subunit (m/z 807.70; C51H92O6Li + ) occurred only from the 10-isomer (TG-EST 3), with an efficiency comparable to the neutral loss of glycerol-linked FA. HCD of lithium adducts ( Figure 3) also opened dissociation channels that were not observed for ammonium adducts. The FAHFA moiety provided an abundant lithiated fragment m/z 543.50 (C34H64O4Li + ), which offered the possibility to characterize FAHFA subunit in the subsequent MS 3 step (see below). Lithium adducts of TG-EST 1 (ω-) and 2 (α-) eliminated both glycerol-linked FAs (m/z 583.53; C37H68O4Li + ) while this pathway was almost absent in the TG-EST 3 (10-isomer). These product ions likely arise following the elimination of the first palmitoleic acid by charge-remote fragmentation, as previously HCD of lithium adducts ( Figure 3) also opened dissociation channels that were not observed for ammonium adducts. The FAHFA moiety provided an abundant lithiated fragment m/z 543.50 (C 34 H 64 O 4 Li + ), which offered the possibility to characterize FAHFA subunit in the subsequent MS 3 step (see below). Lithium adducts of TG-EST 1 (ω-) and 2 (α-) eliminated both glycerol-linked FAs (m/z 583.53; C 37 H 68 O 4 Li + ) while this pathway was almost absent in the TG-EST 3 (10-isomer). These product ions likely arise following the elimination of the first palmitoleic acid by charge-remote fragmentation, as previously suggested for TGs [59] (see in Supplementary Materials, Figure S91). The acylium ion of palmitoleic acid originally bound to glycerol (m/z 237. 22 ), corresponding to the lithium adduct of the HFA, was observed in the spectrum of α-isomer TG-EST 2 ( Figure 3B) and TG-EST 5 ( Figure S92B). This product ion was rationalized as resulting from the neutral loss of the ketene of oleic acid from m/z 543.50 that is the lithium adduct of FAHFA subunit. This proposal was confirmed by the explicit MS 3 of m/z 543.50 shown in Figure 4B. This product ion was determined to be diagnostic of α-estolide-branching regioisomers as evidenced by the wider test-set of HCD spectra of TG-EST 4-8 lithium adducts that are presented in Figure S92.
The ion-trap CID mass spectra of lithium adduct ions yielded fragments identical to those of HCD, with the exception of the absence of low mass ions ( Figure S93). The energyresolved dissociation curves of lithiated TG-EST ( Figures S80-S83) displayed an optimal NCE for MS 2 HCD experiments of 33.
The MS 3 experiments with lithiated FAHFA (m/z 543.50) were performed with the preceding ion trap-CID step providing a more abundant precursor ion than HCD. The MS 3 (CID/CID) spectra of ω-, α-, and 10-isomers (TG-EST 1-3) are shown in Figure 4.  Compared to ammonium adducts, the CID and HCD mass spectra of the lithium adducts allowed a deeper insight into the structure of TG-EST. Abundant lithiated FAHFA species made it possible to determine FA and HFA in the FAHFA moiety for all estolidebranching regioisomers. As in the case of ammonium adducts, clear differentiation of the α-isomers from the others was possible. In summary, the MS 2 HCD and MS 3 CID/CID spectra of lithiated FAHFA made it possible to characterize the FAHFA subunit, i.e., to identify FA, HFA, and the α-estolide-branching site.
OzID has previously been proven to be a useful ion activation method for the structural analysis of lipids and in particular for the resolution of lipid regioisomers [47][48][49][50][51]62]. This technique harnesses ozonolysis reactions of mass-selected ions within the mass spectrometer to identify the location of carbon-carbon double bonds that are either present in the native lipid structure or are formed during preceding ion activation events. As ozonolysis is selective for carbon-carbon double bonds, only highly-specific fragmentations are induced that can provide for unambiguous structure elucidation [45,46]. We employed ozonolysis in a sequential CID/CID/OzID workflow to differentiate estolide-branching site of isomers. The MS 4 spectra of lithiated TG-EST 1-3 are shown in Figure S94. Elimination of FA from FAHFA moiety yields dehydration products [49,63]. In the case of lithiated TG-EST 3 (10-isomer), the CID product ion m/z 261.24 is a mixture of lithiated 9-and 10-Hexadecenoic acids. Their ozonolysis in the final OzID step provided aldehyde ions m/z 179.12 (C 9 H 16 O 3 Li + ) and m/z 193.14 (C 10 H 18 O 3 Li + ) which unambiguously identified n-7 and n-6 carbon-carbon double bond positions and thus also the estolide-branching site (Figure S94C). The product ion m/z 263.22 (C 15 H 28 O 3 Li + ) diagnostic for ω-estolide-branching site was observed in the spectrum of TG-EST 1 ( Figure S94A). OzID did not provide any diagnostic fragment for the α-estolide-branching site in TG-EST 2 ( Figure S94B).
The MS 2 HCD spectra of sodiated TG- The HCD spectra of sodium adducts provided approximately the same structural information as lithium adducts. The ion trap CID of TG-EST sodium adducts showed fragments identical to HCD, with some ions missing due to the low mass cut-off in the ion trap ( Figure S96). Further fragmentation of sodiated ions by collisional activation turned out to be more challenging than for lithium with great competition for dissociation to the bare cation upon CID and thus leading to a lower abundance of lipid-related product ions.
The HCD spectra of sodium adducts provided approximately the same structural information as lithium adducts. The ion trap CID of TG-EST sodium adducts showed fragments identical to HCD, with some ions missing due to the low mass cut-off in the ion trap ( Figure S96). Further fragmentation of sodiated ions by collisional activation turned out to be more challenging than for lithium with great competition for dissociation to the bare cation upon CID and thus leading to a lower abundance of lipid-related product ions.

Mass Spectra of Glycerol sn-Regioisomers
Since the neutral loss of FAs from the sn-1 and sn-3 positions of TGs are equally favored and more competitive than losses from sn-2, the relative abundance of the resulting diacylglycerol-like product ions can be used to infer the regiochemical assignment [59,65].
Here, we compared MS 2 CID and HCD spectra of TG-EST 7 and TG-EST 8 differing by the relative position of FAHFA moiety on the glycerol backbone. As follows from the energy-resolved dissociation curves for [M + NH4] + , [M + Li] + , and [M + Na] + (Figures S79, S83 and S87, respectively), CID provided a relatively stable ratio of fragment peak intensities across a wide range of NCE values (typically NCE [30][31][32][33][34][35]. This was not the case for HCD, where a small change in NCE caused significant changes in product ion abundance. Therefore, CID was used to investigate the relative intensity ratios of peaks corresponding to the neutral loss of palmitic acid and FAHFA units from their positions on glycerol (Figure 6

Mass Spectra of Glycerol sn-Regioisomers
Since the neutral loss of FAs from the sn-1 and sn-3 positions of TGs are equally favored and more competitive than losses from sn-2, the relative abundance of the resulting diacylglycerol-like product ions can be used to infer the regiochemical assignment [59,65].
Here, we compared MS 2 CID and HCD spectra of TG-EST 7 and TG-EST 8 differing by the relative position of FAHFA moiety on the glycerol backbone. As follows from the energy-resolved dissociation curves for [M + NH 4 ] + , [M + Li] + , and [M + Na] + ( Figures  S79, S83 and S87, respectively), CID provided a relatively stable ratio of fragment peak intensities across a wide range of NCE values (typically NCE [30][31][32][33][34][35]. This was not the case for HCD, where a small change in NCE caused significant changes in product ion abundance. Therefore, CID was used to investigate the relative intensity ratios of peaks corresponding to the neutral loss of palmitic acid and FAHFA units from their positions on glycerol ( Figure 6). All the spectra showed a greater peak intensity corresponding to the elimination of palmitic acid (m/z 859.77, C 55  ), while variable across the adducts, was consistently higher where the FAHFA was esterified at sn-1/3 (compared to FAHFA linked to sn-2). The effect was most pronounced in the case of ammonium adducts ( Figure 6A,D). Therefore, the relative abundance of these diagnostic product ions consistently reflected the FAHFA position on the glycerol backbone.
corresponding to the neutral loss of FAHFA (m/z 551.50, C35H67O4 + , for [M + NH4] + ) or lithiated/sodiated FAHFA (m/z 571.53, C36H68O4Li + for [M + Li] + ; and m/z 587.50, C36H68O4Na + for [M + Na] + ), while variable across the adducts, was consistently higher where the FAHFA was esterified at sn-1/3 (compared to FAHFA linked to sn-2). The effect was most pronounced in the case of ammonium adducts ( Figure 6A,D). Therefore, the relative abundance of these diagnostic product ions consistently reflected the FAHFA position on the glycerol backbone. Relying on product ion abundances can lead to ambiguous regiochemical assignments particularly where standard reference materials are not available or in the case of complex samples that may comprise isomeric mixtures. Therefore, we searched for product ions specific to the backbone regiochemistry of TG-EST subunit on glycerol.
Previous investigations have shown that CID of lithium and sodium adduct ions of TGs [50] lead to a five-membered 1,3-dioxolane ring and neutral the loss of one FA. The adjacent FA remains tethered to the glycerol by a newly formed carbon-carbon double bond that can react with ozone and reveal the adjacency of the two FA on the backbone. Analogous MS 3 CID/OzID workflow was used for TG-EST 7 and TG-EST 8. Activation of lithium and sodium adducts in CID resulted in a neutral loss of the palmitic acid originally linked to the glycerol backbone, yielding product ions at m/z 865.78 (C55H102O6Li + ) and m/z 881.76 (C55H102O6Na + ), respectively. When exposed to ozone, the lithiated product ion provided MS 3 (CID/OzID) spectra shown in Figure 7. For both sn-isomers, an ozonide at m/z 913.77 (C55H102O9Li + ) and its oxygen elimination product (m/z 881.77; C55H102O7Li + ) were formed. Oxidative cleavage across the nascent carbon-carbon double bond led to Criegee, aldehyde and carbonate ester product ions indicative of the neutral loss of the FA and/or FAHFA moiety adjacent to the palmitic acid and thus a fragmentation pattern suitable for distinguishing the sn-regioisomers (Figure 7). The TG-EST 7, with the FAHFA moiety in the sn-1/3 position, provided a pair of Criegee and carbonate ester fragments at m/z 687.54 (C40H72O8Li + ) and m/z 671.54 (C40H72O7Li + ), respectively. On the contrary, lithiated TG-EST 8 with the FAHFA moiety in the sn-2 decomposed to two pairs of diagnostic ions, (i) Criegee fragment m/z 557.51 (C35H66O4Li + ) and aldehyde ion m/z 541.51 (C35H66O3Li + ), and Relying on product ion abundances can lead to ambiguous regiochemical assignments particularly where standard reference materials are not available or in the case of complex samples that may comprise isomeric mixtures. Therefore, we searched for product ions specific to the backbone regiochemistry of TG-EST subunit on glycerol.
Previous investigations have shown that CID of lithium and sodium adduct ions of TGs [50] lead to a five-membered 1,3-dioxolane ring and neutral the loss of one FA. The adjacent FA remains tethered to the glycerol by a newly formed carbon-carbon double bond that can react with ozone and reveal the adjacency of the two FA on the backbone. Analogous MS 3 CID/OzID workflow was used for TG-EST 7 and TG-EST 8. Activation of lithium and sodium adducts in CID resulted in a neutral loss of the palmitic acid originally linked to the glycerol backbone, yielding product ions at m/z 865.78 (C 55 H 102 O 6 Li + ) and m/z 881.76 (C 55 H 102 O 6 Na + ), respectively. When exposed to ozone, the lithiated product ion provided MS 3 (CID/OzID) spectra shown in Figure 7. For both sn-isomers, an ozonide at m/z 913.77 (C 55 H 102 O 9 Li + ) and its oxygen elimination product (m/z 881.77; C 55 H 102 O 7 Li + ) were formed. Oxidative cleavage across the nascent carbon-carbon double bond led to Criegee, aldehyde and carbonate ester product ions indicative of the neutral loss of the FA and/or FAHFA moiety adjacent to the palmitic acid and thus a fragmentation pattern suitable for distinguishing the sn-regioisomers (Figure 7). The TG-EST 7, with the FAHFA moiety in the sn-1/3 position, provided a pair of Criegee and carbonate ester fragments at m/z 687. 54   An analogous fragmentation was observed for sodiated TG-EST isomers ( Figure 8). CID/OzID mass spectra of both sodiated and lithiated adduct ions showed the presence of low abundant product ions that were inconsistent with either the putative lipid regiochemistry or the exclusivity of the dissociation mechanisms indicated in Figures 7 and 8, for example, the presence of a small amount of m/z 671.54 in Figure 7B and m/z 687.52 in Figure 8B. These ions point to either a small amount of the alternate regioisomer, perhaps arising from transacylation during synthesis, or the participation of an alternative, but poorly competitive, dissociation pathway. Future work to combine multi-stage ion activation with chromatographic resolution of isomers would be a suitable means to address this ambiguity. An analogous fragmentation was observed for sodiated TG-EST isomers ( Figure 8). CID/OzID mass spectra of both sodiated and lithiated adduct ions showed the presence of low abundant product ions that were inconsistent with either the putative lipid regiochemistry or the exclusivity of the dissociation mechanisms indicated in Figures 7  and 8, for example, the presence of a small amount of m/z 671.54 in Figure 7B and m/z 687.52 in Figure 8B. These ions point to either a small amount of the alternate regioisomer, perhaps arising from transacylation during synthesis, or the participation of an alternative, but poorly competitive, dissociation pathway. Future work to combine multi-stage ion activation with chromatographic resolution of isomers would be a suitable means to address this ambiguity.

Discussion
High-resolution tandem mass spectrometry of ammonium, lithium, and sodium adducts provided important pieces of information on the structure of TG-EST. By selecting the ion type and activation method, it was possible to distinguish TG-EST isomeric species and characterize their structural features. Lithium was determined to be the most useful cationization agent. Lithiated species provided more informative CID and HCD spectra than sodium and ammonium adducts. In the MS 2 , ions permitting the identification of FA, HFA, and FAHFA subunits were present, and some of the lithiated fragments allowed us to characterize branching of the FAHFA moiety. In the MS 3 , all isomers showed loss of FA from lithiated FAHFA ions; abundant ions corresponding to a loss of ketene of FA were additionally present in the α-isomers. The MS 4 with OzID as the last fragmentation step allowed an even more detailed characterization of the estolide-branching site in FAHFA. In this experiment, ozonolysis was used to determine the position of carbon-carbon double bonds formed after elimination of FA from lithiated FAHFA. A similar level of structural information was obtained with sodiated TG-EST. Sodiated species were, however, difficult to fragment in subsequent MS steps because sodium tended to dissociate as bare cation during collisions. Despite their easy formation, ammonium adducts proved less useful as they did not provide FAHFA fragments that could be activated further. All the adducts allowed us to distinguish between sn-1/3 and sn-2 TG-EST isomers. The relative

Discussion
High-resolution tandem mass spectrometry of ammonium, lithium, and sodium adducts provided important pieces of information on the structure of TG-EST. By selecting the ion type and activation method, it was possible to distinguish TG-EST isomeric species and characterize their structural features. Lithium was determined to be the most useful cationization agent. Lithiated species provided more informative CID and HCD spectra than sodium and ammonium adducts. In the MS 2 , ions permitting the identification of FA, HFA, and FAHFA subunits were present, and some of the lithiated fragments allowed us to characterize branching of the FAHFA moiety. In the MS 3 , all isomers showed loss of FA from lithiated FAHFA ions; abundant ions corresponding to a loss of ketene of FA were additionally present in the α-isomers. The MS 4 with OzID as the last fragmentation step allowed an even more detailed characterization of the estolide-branching site in FAHFA. In this experiment, ozonolysis was used to determine the position of carbon-carbon double bonds formed after elimination of FA from lithiated FAHFA. A similar level of structural information was obtained with sodiated TG-EST. Sodiated species were, however, difficult to fragment in subsequent MS steps because sodium tended to dissociate as bare cation during collisions. Despite their easy formation, ammonium adducts proved less useful as they did not provide FAHFA fragments that could be activated further. All the adducts allowed us to distinguish between sn-1/3 and sn-2 TG-EST isomers. The relative abundance of CID fragments corresponding to neutral loss of FAHFA and FA (ammonium adducts) or lithiated/sodiated FAHFA and neutral loss of FA was significantly higher for sn-1/3 isomers.
The effect was pronounced the most in the case of ammonium adducts. The glycerol snregioisomers were also distinguished by CID/OzID of lithium or sodium adducts. Specific peaks in the spectra made it possible to identify the regioisomers unambiguously.
The fragmentation pathways described in this work are the basis for developing new methods for analyzing TG-EST in biological samples using HPLC/MS. A high-resolution mass spectrometer was used to understand the fragmentation pathways. High-resolution instruments are also recommended for analyzing biological samples; however, reliable identification of TG-EST can also be achieved using devices with lower resolving power. CID in QqQ or QTOF-type instruments can be expected to provide similar spectra to HCD discussed in this work. We worked with a concentration of standards of 10 µmol L −1 . Spectra of similar quality can, however, be obtained at significantly lower concentrations ( Figure S97) that are biologically relevant (concentration of TG-EST in the HPLC/MS samples are typically in µmol L −1 range). To comprehensively analyze TG-ESTs in biological matrices, it will be necessary to combine mass spectrometry with efficient chromatography. The HPLC separation of isomeric lipids is a great challenge, especially when it comes to very complex lipid matrices, such as vernix caseosa.