Therapeutic Potential of Leaves from Fridericia chica (Bonpl.) L. G. Lohmann: Botanical Aspects, Phytochemical and Biological, Anti-Inflammatory, Antioxidant and Healing Action

Plants of the species Fridericia chica (Bonpl.) L. G. Lohmann (Bignoniaceae), which are widely distributed in Brazil and named crajiru in the state of Amazonas, are known in folk medicine as a traditional medicine in the form of a tea for the treatment of intestinal colic, diarrhea, and anemia, among other diseases. The chemical analysis of extracts of the leaves has identified phenolic compounds, a class of secondary metabolites that provide defense for plants and benefits to the health of humans. Several studies have shown the therapeutic efficacy of F. chica extracts, with antitumor, antiviral, wound healing, anti-inflammatory, and antioxidant activities being among the therapeutic applications already proven. The healing action of F. chica leaf extract has been demonstrated in several experimental models, and shows the ability to favor the proliferation of fibroblasts, which is essential for tissue repair. The anti-inflammatory activity of F. chica has been clearly demonstrated by several authors, who suggest that it is related to the presence of 3-deoxyanthocyanidins, which is capable of inhibiting pro-inflammatory pathways such as the kappa B (NF-kB) nuclear transcription factor pathway. Another important effect attributed to this species is the antioxidant effect, attributed to phenolic compounds interrupting chain reactions caused by free radicals and donating hydrogen atoms or electrons. In conclusion, the species Fridericia chica has great therapeutic potential, which is detailed in this paper with the objective of encouraging new research and promoting the sum of efforts for the inclusion of herbal medicines in health systems around the world.


Introduction
Fridericia chica (Bonpl.) L. G. Lohmann ( Figure 1) is a liana or creeper that belongs to the family Bignoniaceae, which comprises about 120 genera and 800 species [1]. The largest number of species of this family is found mainly in tropical and subtropical regions, with Brazil and the African continent as the two major centers of geographical distribution. In Brazil, the species of the family Bignoniaceae do not have a unique habitat and are widely distributed in the region of the legal Amazon, as well as throughout the southern region of the country as far down as Rio Grande do Sul. In addition, plants of this family can be found guarajuru, oajuru, oajuru-piranga, paripari, pariri, and piranga, among others [2,6,10]. Its mature leaves, collected from plants ranging from 50 cm to 2 m, are used in the form of tea (dried form), which has an astringent function and can be used against intestinal colic, diarrhea, anemia, uterine inflammation, hemorrhages, leukemia, jaundice, albuminuria, for vaginal hygiene via bathing, or in the form of tincture for topical use directly on skin lesions or even in ointments, creams [10,12], and in the form of soap with an antiacne effect [13]. It is used as an anti-inflammatory, antioxidant, antidiabetic medicine, and a disinfectant [3,10,14,15].
With respect to other classes of natural products, the characterization of terpenes, including carotenoids, and other classes such as fatty acids and their derivatives, tocochromanols and alkaloids, stands out. The diterpene phytol (32), the triterpene squalene (33), and the steroidal β-sitosterol (34) [23] are described as structures of a terpenic nature of up to 30 carbons described in F. chica. On the other hand, a number of tetraterpenes have been identified using HPLC-MS, among them the main compounds β-carotene (35), (all-E)-zeaxanthin (36), (all-E)-lutein (37), (all-E)-violaxathin (38), (13Z)-violaxathin (39), and (all-E)-luteoxanthin (40) [22]. In addition to antioxidant carotenoids, tocochromanol α-tocopherol (41) has been described as well [23]. Additionally, the identification of the fatty acid ethyl ester ethyl palmitate (42) has been reported [23], as has the alkaloid pheophorbide α (43) [24]. In Table 1, the 43 compounds already identified in F. chica and their biological activities already studied from these molecules alone (isolated from F. chica extract or other sources) are shown. It is worth noting that despite the tea being the most popular use of F. chica, phytochemical studies were focused on hydroalcoholic and/or organic extracts, possibly aiming for the enhancement of extraction yields. Moreover, tea preparations may contain the most polar compounds, specifically the positively charged 3-deaoxyanthocyanins, information that is corroborated by the bright red color of the dried leaf infusions. Thus, the composition of the extract is of great importance, as these compounds can exert pharmacological effects with summative, potentiating or deleterious characteristics ( Figure 3).  With respect to other classes of natural products, the characterization of terpenes, including carotenoids, and other classes such as fatty acids and their derivatives, tocochromanols and alkaloids, stands out. The diterpene phytol (32), the triterpene squalene (33), and the steroidal β-sitosterol (34) [23] are described as structures of a terpenic nature of up to 30 carbons described in F. chica. On the other hand, a number of tetraterpenes have been identified using HPLC-MS, among them the main compounds βcarotene (35), (all-E)-zeaxanthin (36), (all-E)-lutein (37), (all-E)-violaxathin (38), (13Z)violaxathin (39), and (all-E)-luteoxanthin (40) [22]. In addition to antioxidant carotenoids, tocochromanol α-tocopherol (41) has been described as well [23]. Additionally, the identification of the fatty acid ethyl ester ethyl palmitate (42) has been reported [23], as has the alkaloid pheophorbide α (43) [24]. In Table 1, the 43 compounds already identified in F. chica and their biological activities already studied from these molecules alone (isolated from F. chica extract or other sources) are shown. It is worth noting that despite the tea being the most popular use of F. chica, phytochemical studies were focused on hydroalcoholic and/or organic extracts, possibly aiming for the enhancement of extraction yields. Moreover, tea preparations may contain the most polar compounds, specifically the positively charged 3-deaoxyanthocyanins, information that is corroborated by the bright red color of the dried leaf infusions. Thus, the composition of the extract is of great importance, as these compounds can exert pharmacological effects with summative, potentiating or deleterious characteristics ( Figure 3).

Pharmacological Properties
Historically, plants have been an important source of active ingredients for drug development, and scientific research has been directed towards the prospect of new drugs based on natural products [83]. Thus, the connection between plants and health is responsible for the beginning of a new generation of therapy that integrates drugs derived from plants or the use of plants themselves or their parts [84]. The public health system in Brazil offers twelve herbal medicines as treatment options, among them several with anti-inflammatory potential, such as Aloe vera, Schinus terebenthifolius, and Uncaria tomentosa [85,86].
The use of plants as a medicine occurs in many traditional societies [87]; therefore, it is important to stimulate scientific studies that prove existing popular knowledge about plants and their effectiveness in the treatment of diseases [88]. In this sense, several studies have been carried out with different extracts of F. chica leaves in order to prove the effectiveness of the plant for the therapeutic purposes for which it is popularly used ( Table 2).  Among biological effects from F. chica leaf extracts, anti-inflammatory, antioxidant, and healing properties are well evidenced from previous studies (Table 2), and result from the leaves' phytochemical composition. Studies have shown that the compounds identified in F. chica leaf extracts, as well as in several other plants, are responsible for the above-mentioned pharmacological activities (Table 1) when evaluated in isolation (Table 1).

Healing Activity
The tissue repair process is divided into three successive and overlapping phases that present specific characteristics: (1) the inflammatory phase, (2) the proliferative phase, and (3) the remodeling phase. In the inflammatory phase, there is an increase in vascular permeability, which promotes chemotaxis and the consequent intense migration of leukocytes, mainly neutrophils and macrophages, to the site of tissue damage in response to the release of pro-inflammatory chemical mediators. The proliferative phase is characterized by the reconstitution of epithelial tissue, angiogenesis and migration of endothelial cells, granulation tissue formation, and extracellular matrix deposition, mainly collagen produced by activated fibroblasts. In the remodeling phase, the initial deposited collagen (type III) is degraded by collagenases and reabsorbed, and the gradual replacement of type III collagen by type I collagen occurs until the proportion found in healthy tissue is reached, providing reorganization of the extracellular matrix and marking the success of tissue repair [110,111].
Several herbal medicines have been explored in order to evaluate their action on tissue healing, mainly due to their lower cost and fewer observed adverse effects [112]. Tissue regeneration studies with F. chica leaves have addressed the action of different extracts and formulations in both in vitro and in vivo experiments using experimental animals in order to investigate different phases of the healing process. Among the main experimental procedures in the bioprospecting of new components with healing potential, cell proliferation assays using fibroblasts and the production of extracellular matrix components such as collagen are considered highly reliable models. Fibroblasts are connective tissue cells that are essential for the formation of the dermis and are responsible for structural firmness. After injury, fibroblasts proliferate and migrate to the injured site, where they produce a large amount of extracellular matrix material, such as type I and III collagen, which helps to isolate and repair the injured tissue. The use of a methanolic extract of F. chica leaves stimulates the growth of fibroblasts in vitro in a concentration-dependent manner and increases collagen production [14].
From this tissue regeneration property, several in vivo studies with experimental animals have been developed to evaluate the healing potential of F. chica in different tissue types. In treatment of gastric ulcers induced by ethanol and indomethacin in Wistar/Uni rats, administration of the hydroalcoholic extract of F. chica leaves reduced about 60% of lesions [113]. The methanolic extract administered orally was able to reduce up to 96% of the ulcerative effect of ethanol in a murine model [14]. The ethanol-induced model is associated with gastric mucosal injury induced by oxidative stress (via alcohol); thus, the gastroprotective effect of F. chica may occur via antioxidant action. In skin lesions of Swiss mice, treatment with ethanolic extract of F. chica leaves has not yet shown promising results. During 21 days of observation, it was found that the extract did not accelerate total tissue regeneration and presented a profile that was similar to the control group [90]. Other studies have investigated the effect of F. chica leaves on the healing process of tendon injuries in rats. Observed results included an increase in the total collagen content on the seventh and twenty-first day of the healing process, an increase in the synthesis of collagenase type IV (matrix metalloproteinase-2, an enzyme that participates in the extracellular matrix remodeling process), and better organization of collagen fibers. The treated groups presented a lower inflammatory reaction, which promoted the recovery of the injured animals' ability to walk [114,115].
In addition to the studies developed in different tissues, investigations of the healing properties of Fridericia chica have used different types of formulations for administration of the plant extract and for carrying out these studies. The choice of formulation is ex-tremely important, as it influences the stability, distribution, solubility, bioavailability, and protection of the active compound. Topical formulations such as cream and gel containing F. chica leaf extract are being developed for the treatment of skin lesions [90,116]. For the treatment of gastric ulcers, a nanoformulation of F. chica extract has been elaborated in chitosan triphosphate-based nanoparticles for oral administration. Nanoformulation was responsible for a greater fibroblast proliferation effect, and the anti-ulcerogenic effect of F. chica leaf extract in a nanoformulation was more efficient compared to hydroalcoholic extract [113].
From the 43 compounds identified from F. chica leaves, seven molecules (comprising the classes of flavones, flavonols, terpenes, tochromanols, and alkaloids) have been described to present healing properties when evaluated alone. Among them, kaempferol (compound 24- Table 1) is a flavonol which has been described as improving repair of diabetic excisional and nondiabetic incisional wounds in rats, working as an effective topical wound healing agent [52]. Another component present in F. chica leaf extract in considering amount is α-tocopherol (compound 41, Table 1), belonging to the tocochromanol class. Shuid and colleagues [78] evaluated the effects of α-tocopherol supplementation on osteoporotic fracture healing in a rat model, showing an improvement in fracture healing associated with the antioxidatant property. Other compounds have been shown to induce healing activity, such as apigenin (compound 17, Table 1), with anti-hyaluronidase and anti-collagenase activities [41], scutellarin (compound 21, Table 1) with angiogenic properties [49], and pheophorbide a (compound 43, Table 1), which decreased the length of the inflammation stage [82].

Anti-Inflammatory Activity
Inflammation is defined as a protective response of the host to various interactions with the external environment. The assembly of this response requires the participation of cells and molecules, including macrophages and neutrophils, along with cytokines, such as interleukin (IL)-1β and IL-6 [117][118][119][120][121]. These cells recognize and discriminate stimuli as damage-associated molecular patterns (DAMPs) and pathogen-associated molecular patterns (PAMPs) through pattern recognition receptors (PRR) [122]. The inflammatory response, despite being an effective defense mechanism, can occur in an exacerbated or perpetuated manner for a longer period of time than is necessary, causing important tissue damage and contributing to the development of diseases such as rheumatoid arthritis [123,124].
The participation of inflammation in the pathophysiology of multiple diseases stimulates the constant development of anti-inflammatory drugs, and nature represents an important source of plants with therapeutic potential that needs to be explored [125,126]. Plants such as Aloe vera have demonstrated efficacy in controlling the inflammatory response and are considered by the Brazilian public health system as a treatment option [127]. Flavonoids, a class of secondary metabolites belonging to the group of phenolic compounds, are anti-inflammatory agents and can act to block arachidonic acid cascade through inhibition of the COX and lipoxygenase pathways [128,129]. The species Fridericia chica is used in traditional medicine as an anti-inflammatory agent, which has been confirmed in several studies using different extracts prepared from the leaves of this plant [15,17,130].
The chemical characterization of extracts of F. chica leaves has allowed the identification of 3-deoxyanthocyanidins, to which are attributed the greater part of the anti-inflammatory effect of this plant [4,17,18]. In 2001, Zorn et al. [17] demonstrated that both the lipophilic extract of Fridericia chica leaves (200 mg/mL) and one of its isolated components (carajurine) at a concentration of 500 µM had the ability to completely inhibit nuclear factor kappa B (NF-κB), which is responsible for the transcription of genes that encode several proinflammatory mediators and which participates in the pathogenesis of diseases such as multiple sclerosis [131][132][133]. Several authors have suggested that other unidentified substances present in the extract of F. chica leaves may contribute to the anti-inflammatory activity of this species [17].
Several models of inflammation have been used for the study of anti-inflammatory drugs, one of which is the experimental model of inflammation induced by snake venom. Its pathogenesis may involve the participation of the enzyme phospholipase A 2 , which stimulates the release of arachidonic acid [134,135]. In 2009, Oliveira et al. [15] evaluated the anti-inflammatory effect of an aqueous extract of the leaves of Fridericia chica in mice inoculated with the venom of the snakes Bothrops atrox and Crotalus durissus ruruima, administered via the subcutaneous and intraperitoneal routes at different times. After treatment with the extract, it was possible to observe that it showed inhibitory activity, especially in the processes of myocytolysis and granulocyte migration, thus resulting in a decrease in inflammation. This effect is attributed to substances present in the extract, flavonoids among them.
The effectiveness of Fridericia chica leaves as an anti-inflammatory agent has been demonstrated in other models, such as inflammatory angiogenesis, which was induced in a murine model by a sponge implant (polyether-polyurethane) and in the proliferative process of human tumor cell lines in vitro, in which Michel et al. (2015) [130] observed a reduction in the amount of neutrophils at the site of the sponge implants and a great decrease in angiogenesis after treatment with aqueous and ethanolic extracts. Flavone escutelarin derivatives, found in the hydroethanolic extract of Fridericia chica leaves, have been associated with a reduction of parameters linked to inflammatory response in the model of intestinal mucositis induced by 5-fluorouracil in a study by [136].
Among phytochemical compounds present in F. chica leaf extracts, 25 have been described as presenting anti-inflammatory behavior ( Table 1). The anthocyanins comprise the class of most abundant compounds in F. chica leaves, with carajurin composing one of the major molecules found in extract of the leaves [17]. Carajurin (compound 1, Table 1) is capable of inhibiting transcription factor NF-κB, an important signaling pathway associated with pro-inflammatory response [17]. F. chica leaf extracts consist of a high range of flavonoid compounds, such as flavones and flavonols (Table 1). Regarding this class, the literature on their biological potentials is extensive, with a total of 14 flavonoids from F. chica leaf extracts having been shown to suppress inflammation through different cell signaling pathways. An example is luteolin (compound 8, Table 1), a flavone which has been shown to interact with several inflammatory targets such as matrix metalloprotease 9 (MMP9) and mitogen-activated protein kinase 1 (MAPK1) [25]. Chrysoeriol (compound 9, Table 1), another flavone, ameliorates TPA-induced ear edema in mice, and its inhibition of JAK2/STAT3 and IκB/p65 NF-κB pathways is involved in the anti-inflammatory effects [27]. Hispidulin, a flavone found in a number of plants, among them in F. chica, was evaluated by Yu et al. (2020) [36] in relation to protection against neuroinflammation using the immortalized cell line of murine microglial BV2 (BV2 cells). Hispidulin has been shown to inhibit NO and ROS production, which suppressed the expression of the inflammationrelated enzymes iNOS and COX-2 [137] in a dose-dependent manner. Corroborating these data, Torres et al. (2018) [138] showed a decrease in the enzymatic activity of lipoxygenase in the presence of ethanolic extract of F. chica leaves. They observed that treatment with hispidulin in the presence of LPS inhibited the production of TNF-α, IL-6, IL-1 β, and PGE2 in a dose-dependent manner. In addition, hispidulin showed activity in reducing NF-κB, and decreased the levels of phosphorylation of IκB kinase in BV2 cells. Based on the above examples, there is other evidence of the effect of the species F. chica leaves in the modulation of the cytokine profile, among them that demonstrated by Lima (2020) [139], which suggests an increase in the production of IL-10 and a decrease in the production of IL-1β in RAW 264.7 cells activated with LPS in the presence of a hydroethanolic extract of F. chica. The authors attributed this effect to 5 O-methylescutelarin, a derivative of the flavone escutelarin. The aforementioned studies corroborate the therapeutic potential described in traditional medicine for F. chica.
Despite being encountered in minor proportions in F. chica, terpenes may play roles in its biological activities, including in anti-inflammatory response. β-carotene (compound 35, Table 1) was capable of inhibiting LPS-stimulated Cox2, Nos2, and Tnfalpha gene expression from macrophages in vitro [69]. Another terpene, phytol (compound 32, Table 1), has been described as inducing anti-inflammatory activity in vitro and in vivo, with higher potency in the presence of others non-steroidal anti-inflammatories [64]. Among fatty acids, ethyl palmitate (compound 42, Table 1) reduced plasma levels of tumor necrosis factor-α (TNF-α) and IL-6, decreased NF-κB expression in liver and lung tissues, and ameliorated histopathological changes in an LPS-induced endotoxemia rat model [79]. The alkaloid pheophorbide a (compound 43, Table 1), was found to inhibit nitric oxide production and suppress the expression of iNOS proteins in LPS-stimulated macrophages [80].

Antioxidant Activity
The term oxidative stress, first proposed in 1985, refers to the imbalance between the production of oxidizing and antioxidant molecules; it favors oxidation reactions and compromises cell signaling and redox control [140][141][142]. The aforementioned changes result in excessive production of free radicals, which can contribute to the generation of reactive oxygen species, thus resulting in structural damage to biological systems [143][144][145]. Reactive species are defined as atoms, molecules, or ions resulting from oxygen, usually with a high reactivity capacity, which can cause damage to cellular constituents, such as lipid peroxidation and oxidation of DNA bases [146][147][148][149]. Free radicals and other reactive species are essential for cellular homeostasis, but when generated excessively they contribute to cellular aging and the development of diseases, as is observed in the development of hemolytic anemia due to deficiency of the enzyme glucose-6-phosphate dehydrogenase. In addition, oxidative stress is part of the pathophysiology of several chronic degenerative diseases, including cancer, diabetes, and hypertension [150][151][152][153][154][155][156].
Antioxidants are compounds that are capable of slowing, preventing, or removing damage caused by redox imbalance, and thereby regulating the development of oxidative stress [157]. The large number of adverse effects observed with the use of synthetic antioxidants have driven the search for other alternatives, among them substances isolated from natural products [158][159][160]. The main antioxidants present in plants are phenolic compounds, which represent a family of secondary metabolites with the property of interrupting chain reactions caused by free radicals by donating hydrogen atoms or electrons [161][162][163]. To this large group of compounds belong several of the flavonoids, which are present in medicinal plants such as the species F. chica [20,21,91,[164][165][166].
The antioxidant potential of F. chica has already been evaluated in several studies. In 2012, Do Amaral et al. [109] identified the presence of phenolic compounds in an ethanolic extract of the leaves of this plant. In addition, Gemelli et al. (2015) [166] and Campos de Siqueira et al. (2019) [22] examined the antioxidant potential in the aqueous and hydroethanolic leaf extract, respectively. The anti-inflammatory property of 3-deoxyanthocyanidins, which are phenolic pigments with the ability to stabilize free radicals by donating hydrogen radicals, was proposed by Zorn et al. in 2001 [17]. However, the antioxidant capacity of these flavonoids was identified much later, by Do Amaral et al. in 2012 [109], and later confirmed by Dos Santos et al. in 2013 [166]. Another important finding was that reported by Olivero-Verbel et al. in 2021 [167], which suggests the ability of aqueous extract of F. chica leaves to induce nuclear translocation of DAF-16 in a prominent manner, thus suggesting the potential of this extract in the regulation of oxidative stress [17,22,109,[166][167][168].
As already mentioned, flavonoids are phenolic compounds which have a great antioxidant potential. Among them are flavones, the antioxidant capacity of which is related to the presence of free hydroxyl groups in their A and B rings [161,162]. Luteolin (compound 8- Table 1), one of the most abundant flavones, was identified by Do Amaral et al. (2012) [116], who evaluated the antioxidant activity of the ethanolic extract of F. chica leaves in the presence of the free radical DPPH (2,2-diphenyl-1-picrylhydrazil) using Gingkgo bibola as a positive control and demonstrated that the antioxidant capacity of F. chica extract is superior. This antioxidant effect was attributed mainly to the flavones luteolin and apigenin. In 2015, Gemelli et al. [168] confirmed the presence of luteolin in the aqueous extract of F. chica leaves and demonstrated its antioxidant potential by DNA damage assay in CHO cells (hamster ovary cells) [109,168].
The leaves of Fridericia chica have a high flavones content along with the presence of apigenin (compound 17, Table 1), with high antioxidant activity [40], as evidenced by Siraichi et al. in 2013 [21], confirming what had already been proposed by Do Amaral et al. in 2012 [109]. Other important flavone identified in the extract of F. chica leaves are scutellarin (compound 21, Table 1) and scutellarein (compound 14, Table 1), which are present in other medicinal plants such as Scutellaria barbata and S. lateriflora. Scutellarin is a glucoronid form of scutellarein, which means that although both molecules present antioxidant activity, the aglycone form (scutellarein) has a stronger activity [34]. The research of Campos de Siqueira et al. (2019) [22], who identified carotenoids such as βcarotene and α-carotene in a hydrometanolic extract of F. chica, was not the first time that escutelarin was identified in the species F. chica, as in 2013 Siraichi et al. [21] attributed the antioxidant effect of the species to the presence of this flavone [21,22,109].
Terpenes are well known antioxidant agents, and have been shown to provide relevant protection under oxidative stress conditions. The terpene zeaxanthin (compound 36, Table 1) protects against chronic eye and cardiovascular diseases due its antioxidant property by directly quenching reactive oxygen species (ROS) and by facilitating glutathione synthesis [72]. Violaxanthin (compound 38, Table 1) possesses potent lipid peroxidation inhibitory activity [76]. Other compounds from different classes, such as α-Tocopherol and Pheophorbide a (compounds 41 and 43, respectively, in Table 1) have shown antioxidant activity as well, and are present in others plant species [77,81]. Martins [170], demonstrated the antioxidant capacity of a hydroethanolic extract of Fridericia chica in the presence of both the free radical DPPH and the oxidative damage induced by ultraviolet radiation. The use of several methodologies to evaluate the effects of F. chica extracts for the control of oxidative stress allowed for a more complete view of this mechanism. Other authors, such as Torres et al. in 2018 [138] and Teixeira et al. in 2017 [171], have included other free radical neutralization assays, such as ABTS (2,2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) and FRAP (ferric reducing antioxidant power), thus reaffirming the biological effects of F. chica [138,[169][170][171].
In 2009, the Brazilian Ministry of Health published a list of 71 species that are part of the Report on Medicinal Plants of Interest to the SUS (RENISUS); F. chica is among them due to the large amount of scientific evidence pointing to its different biological effects [172,173]. One of the most important biological effects of this species is the antioxidant effect, which can contribute to the control and prevention of degenerative diseases, as already mentioned. Encouraging the development of research related to F. chica is be essential for its inclusion in the list of herbal medicines prescribed within the Brazilian health services, through which twelve herbal medicines are already available that are part of the National List of Essential Medicines (RENAME) [127].

Conclusions
The World Health Organization (WHO) has encouraged the inclusion of medicinal plants in primary health care networks, and in Brazil their use is regulated by the National Health Surveillance Agency (ANVISA). The public health system of Brazil has offered herbal medicines since 2007, and 12 herbal medicines have been made available in the public network by the Ministry of Health (MS). The species Fridericia chica (Bonpl.) L. G. Lohmann is traditionally used in folk medicine, especially in the Amazon region, and is part of the 71 species of interest to the SUS (Brazilian public health system). Among these species, Aloe vera, Schinus terebenthifolius, and Uncaria tomentosa are used as commercial herbal pharmaceutical formulations in the Brazilian Health System. Schinus terebenthifolius has healing and anti-inflammatory action and is used as an antiseptic topical for gynecological applications, available in the forms of cream and ovules. Uncaria tomentosa is indicated as an assistant in cases of arthritis and osteoarthritis thanks its anti-inflammatory and immunomodulatory activity, and is available in capsule, pill, and gel forms. Aloe vera is indicated for the topical treatment of first and second degree burns and as an adjunct in cases of psoriasis vulgaris, and is available in cream form [85]. Therefore, the present review indicates the potential inclusion of F. chica on the list of herbal medicines prescribed within the scope of Brazilian Health System services, as the extract can be used in different pharmacological formulations such as topical or oral administrations in order to treat diseases associated with inflammatory response and wounded tissue.
Scientific evidence indicates the different biological effects of F. chica, which proves the therapeutic effect of this species in folk medicine, which is explained especially by the presence of a wide range of biologically active compounds that are known from other plant species. The present review has shown that from all compounds identified in F. chica leaf extract, over 70% are known to exert anti-inflammatory, antioxidant, and/or healing properties based on previous studies. Therefore, the pharmacological properties of F. chica leaf extract are directly associated with the phytochemical composition, with different molecules working in association in order to observe the conclusive therapeutical potential. Although carajurin is found as the major component of the extract with anti-inflammatory activity, its synergism with other less abundant components is important to the overall pharmacological properties of the extract. Therefore, the proportion of every molecule within the extract and its biological potency should be more closely investigated. It is of great importance to encourage more studies related to the chemical profile, standardization of extracts, and proof of pharmacological activities of F. chica. Concerning the traditional use of F. chica leaf extract as tea, more studies should be performed in order to evaluate the phytochemical composition of aqueous infusions, considering that almost all studies on leaves have used extract compositions with other types of solvents. New research needs to be developed in order to add scientific evidence that favors the use of this and other medicinal species, both in Brazil and in other regions of the world.
The scientific investigations cited in this study contribute to the construction of arguments, providing a solid basis to attribute anti-inflammatory, antioxidant, and healing properties to Fridericia chica, thus demonstrating the scientific bases that justify its use in traditional medicine.