Identification of miRNA-Target Gene Pairs Responsive to Fusarium Wilt of Cucumber via an Integrated Analysis of miRNA and Transcriptome Profiles

Fusarium wilt (FW) of cucumber (Cucumis sativus L.) caused by Fusarium oxysporum f. sp. cucumerinum (Foc) is a destructive soil-borne disease that severely decreases cucumber yield and quality worldwide. MicroRNAs (miRNAs) are small non-coding RNAs (sRNAs) that are important for regulating host immunity because they affect target gene expression. However, the specific miRNAs and the miRNA/target gene crosstalk involved in cucumber resistance to FW remain unknown. In this study, we compared sRNA-seq and RNA-seq data for cucumber cultivar ‘Rijiecheng’, which is resistant to FW. The integrated analysis identified FW-responsive miRNAs and their target genes. On the basis of verified expression levels, we detected two highly expressed miRNAs with down-regulated expression in response to Foc. Moreover, an analysis of 21 target genes in cucumber inoculated with Foc indicated that JRL3 (Csa2G362470), which is targeted by miR319a, and BEE1 (Csa1G024150), DAHP1 (Csa2G369040), and PERK2 (Csa4G642480), which are targeted by miR6300, are expressed at high levels, but their expression is further up-regulated after Foc inoculation. These results imply that miR319a-JRL3, miR6300-BEE1, miR6300-DAHP1 and miR6300-PERK2 regulate cucumber defenses against FW, and provide the gene resources that may be useful for breeding programs focused on developing new cucumber varieties with enhanced resistance to FW.

During an infection, Foc easily penetrates cucumber plants and quickly spreads to the vascular tissues, where it occludes xylem vessels and produces a toxin that kills host cells, ultimately leading to FW. The disease results in the wilting of leaves or even the entire plant, with plant death occurring several days or weeks after infection [6,7]. Fusarium wilt is extremely difficult to control because it can occur during all cucumber growth stages [2]. Additionally, Foc can survive in the soil, straw or seeds for many years or even decades, leading to long-term disease cycles [8,9]. Furthermore, the changeable pathogenicity of Foc has limited the effectiveness of certain fungicides [3,10]. The most sustainable way to control FW involves the development and application of resistant cultivars. Thus, effective resistance genes need to be identified and the mechanism mediating disease resistance must be thoroughly characterized to develop FW-resistant cucumber cultivars. Plant microRNAs (miRNAs) are endogenous small non-coding RNAs (sRNAs) that serve as crucial molecular regulators of the expression of functional genes by targeting mRNAs or by inhibiting translation [11][12][13]. Plant miRNAs participate in almost all biological processes, such as growth and development [14,15], hormone signal transduction [16] and responses to abiotic and biotic stresses (e.g., pathogen attack) [17,18]. For example, in rice, overexpression of Osa-miR812w increases resistance to Magnaporthe oryzae, whereas CRISPR/Cas9-mediated miR812w editing enhances disease susceptibility, suggesting that miR812w positively contributes to blast resistance [18]. In cotton, cleavage of GhNAC100 mRNA by ghr-miR164 decreases the amount of GhNAC100 that can bind to the CGTA-box of GhPR3 promoter to repress expression, thereby enhancing the resistance to Verticillium dahliae [19]. Tomato lncRNA23468 functions as a decoy RNA for miR482b to modulate the expression of NBS-LRR genes, resulting in increased resistance to Phytophthora infestans [20]. These results suggest that miRNAs mediate crucial plant defense gene regulatory pathways. Therefore, resistance-related miRNAs must be identified and their target genes associated with responses to pathogens should be functionally characterized. However, to date, very few miRNA-seq and RNA-seq analyses of cucumber defense responses to FW have been conducted. Thus, the miRNAs associated with FW resistance and their underlying mechanisms in cucumber remain unknown.
On the basis of an earlier examination of germplasm resources inoculated with Foc, we had confirmed that the cucumber cultivar 'Rijiecheng' was high-resistant against Foc, and might possess the resistant genes performing Foc defense [21]. In this study, we performed the integrated miRNA-seq and RNA-seq analyses to identify the FW-responsive miRNAs and their target genes in the FW-resistant cucumber cultivar 'Rijiecheng'. Through the miRNA-seq from the Rijiecheng roots infected with Foc, we identified that eleven differentially expressed miRNAs (DEMs) were related to FW resistance. We further found that two miRNAs (miR319a and miR6300) had high expression levels and were downregulated expression affected by the inoculation with Foc. Followed the RNA-seq analysis of Rijiecheng infected with Foc, 21 corresponding target genes of miR319a and miR6300 had adverse expression tendencies and were expressed differently in cucumber roots inoculated with Foc. Based on the expression verification, we determined that JRL3 (Csa2G362470), which is putatively targeted by miR319a, and BEE1 (Csa1G024150), DAHP1 (Csa2G369040), and PERK2 (Csa4G642480), which are putatively targeted by miR6300, were abundantly expressed, with expression levels that were higher than those in the controls. These findings indicate that miR319a-JRL3, miR6300-BEE1, miR6300-DAHP1 and miR6300-PERK2 might be important for cucumber defenses against FW. Moreover, they may be useful for generating FW-resistant cucumber cultivars via breeding.

Plant Materials and Foc Treatment
This study was conducted using cucumber cultivar 'Rijiecheng', which was confirmed to be resistant to Foc during an earlier examination of germplasm resources inoculated with Foc [21]. Seeds were germinated on wet gauze in a Petri dish at 28 • C. The resulting seedlings were incubated in a growth chamber with the same conditions of 25 • C/18 • C cycle under a 16 h light/8 h dark cycle.
The Foc strain used in this study was isolated from cucumber roots exhibiting FW symptoms and then propagated on potato dextrose agar with different antibiotics inhibiting bacteria growth in plates at 28 • C for 4 days. The conidia were harvested and isolated for several times. Followed the inoculation phenotypes and sequencing verification, the Foc strain was confirmed and then cultured in potato dextrose broth in plates on a shaker (180 rpm) at 28 • C for 3 days. The concentration was adjusted to 10 6 spores/mL using sterile distilled water prior to the inoculation of 14-day large cucumber seedlings at second true-leaf stage via a published dip-inoculation method [22]. Three replicates of seedling roots were harvested at 0, 24, 48, 96, and 192 h after the Foc inoculation. All samples were flash-frozen in liquid nitrogen and stored at −80 • C until analyzed.

Total RNA Isolation and Library Preparation for sRNA Sequencing
We collected three replicates of Foc-inoculated cucumber roots at different postinoculation time-points (0, 48, and 96 h); non-inoculated roots were collected as the controls refer to the method as described by Dong and associates (2020) [22]. Total RNA for the real-time polymerase chain reaction (PCR) was extracted from flash-frozen cucumber roots using the MiniBEST Plant RNA Extraction Kit (TaKaRa, Dalian, China), after which RNA degradation and contamination were monitored on 2% agarose gels. Additionally, RNA purity was assessed using the NanoPhotometer ® spectrophotometer (IMPLEN, Calabasas, CA, USA), whereas the RNA concentration was determined using the Qubit ® RNA Assay Kit and the Qubit ® 2.0 Fluorometer (Life Technologies, Gaithersburg, MD, USA).
For each sample, 3 µg total RNA was used as the input material for constructing sRNA libraries. The sequencing libraries were generated using the NEBNext ® Multiplex Small RNA Library Prep Set for Illumina ® (New England Biolabs, Beverly, MA, USA) and index codes were added to attribute sequences to each sample. The sRNA sequencing analysis was completed using the AllPrep DNA-RNA-miRNA Universal kit (Qiagen, Duesseldorf, Germany), with DNA contaminants removed by an on-column DNase treatment.

miRNA-Seq Analysis
The miRNA sequencing analysis was performed by Novogene using the Illumina NextSeq 500 system. Expression levels were calculated as the number of transcripts per million (TPM). To identify the known miRNAs, the clean reads were used as queries for a BLAST search of the miRNA database miRbase 21.0 (http://www.mirbase.org/, accessed on 7 September 2021). The sRNA tags were mapped to the reference sequence using Bowtie (with no mismatches) to analyze their expression and distribution on the reference sequence [23]. We analyzed the miRNA families and their sequence conservation in miRbase, and the DEMs were further screened according to the following criterion: |log 2 FPKM (fold-change)| > 1 and p value < 0.05. For the unannotated sequences, we predicted new miRNAs using mireap (http://sourceforge.net/projects/mireap/, accessed on 7 September 2021). As miRNAs are primarily bound to the target site by complementary pairing, the data were analyzed using Miranda (http://www.microrna.org/microrna/ home.do, accessed on 7 September 2021) to identify the targets of the mature miRNA sequences. The sRNA raw data were deposited in the NCBI Sequence Read Archive (accession number PRJNA760453).

MicroRNA Family Identification and Target Prediction
The miRNA families in other species were identified. In our analysis pipeline, known miRNAs were used along with miRNA.dat (http://www.mirbase.org/ftp.shtml, accessed on 7 September 2021) to search for families, whereas novel miRNA precursors were submitted to Rfam (http://rfam.xfam.org/, accessed on 7 September 2021) to screen for Rfam families. The miRNA expression levels were estimated as the number of TPM according to the following criteria [24]. The differential expression between two conditions/groups was analyzed using the DESeq R package (1.8.3). The p-values were adjusted according to the Benjamini and Hochberg method. A corrected p-value of 0.05 was set as the threshold for determining significant differences in expression. The target genes of miRNAs in plants were predicted using psRobot_tar in psRobot [25].
The TargetFinder software was used to predict miRNA target genes [26]. The candidate target genes of the DEMs were functionally characterized by a Gene Ontology (GO) enrichment analysis on the basis of the genome annotate of cucumber (http://cucurbitgenomics. org/organism/20, accessed on 7 September 2021).

Quantitative Real-Time RT-PCR (qRT-PCR) Assay of the miRNAs
The known and novel miRNAs were assayed by qPCR to validate relative expression patterns. The reverse transcription reaction was performed using the miRNA 1st Strand cDNA Synthesis Kit (by stem-loop) (Vazyme, Nanjing, China). The qPCR analysis was Biomolecules 2021, 11, 1620 4 of 12 performed using the miRNA Universal SYBR ® qPCR Master Mix (Vazyme, Nanjing, China), with U6 snRNA used as the internal control. The miRNAs gene expression was calculated using qRT-PCR analysis, and expression data represent as the 2 −∆Ct method followed by further statistical analysis [27]. The standard deviation was measured for three biological replicates. The primers of the miRNAs used in the experiment were designed by miRNA Design V1.01 software and listed in Additional file 1: Table S1.

Identification and Validation of Target Differentially Expressed Target Genes
We had analyzed the transcriptome of Foc-inoculated cucumber roots collected at different time-points after inoculation (0, 24, 48, 96, and 192 h). The generated data were submitted to the NCBI database (accession number PRJNA472169). The differentially expressed genes were considered with an adjusted FDR < 0.01 identified by DESeq and |log 2 FPKM (fold-change)| ≥ 1. The transcriptome was used to further screen for target genes of the miRNAs. Differences in the expression of the candidate genes were indicated by the color scale of the Toolbox for Biologists software.
The expression verification of the candidate genes was performed by the qRT-PCR analysis in the cucumber cultivar 'Rijiecheng' infected with Foc. The RNA samples of the Foc-inoculated cucumber roots were reverse transcribed into cDNA using the HiScript Q RT SuperMix for qPCR (Vazyme, Nanjing, China). The target gene-specific qRT-PCR primers were designed using the Beacon Designer 7.0 software. The cucumber tubulin alpha chain gene (Csa4G000580) was used as the internal reference control. The qRT-PCR analysis was performed using the Iqtm5 Multicolor qPCR detection system (Bio-Rad, Hercules, CA, USA) and the AceQ SYBR Green Master Mix (Vazyme, Nanjing, China), with three technical replicates per biological replicate. The expression data for three biological replicates were analyzed and are presented herein as the mean ± standard deviation. Primer information is provided in Additional file 1: Table S1.

Identification of miRNAs Responsive to Foc
To identify the miRNAs involved in plant responses to Foc, 15 sRNA libraries for three biological replicates of the Foc-inoculated roots at 48 and 96 h post-inoculation and the non-inoculated controls were prepared for a high-throughput sequencing analysis to identify miRNAs. We used the 18-to 24-nt sRNA sequences as queries to search for matches among the plant miRNA sequences in the miRBase 21.0 database. A total of 1185 miRNAs, including 1115 known miRNAs and 70 novel miRNAs, were identified in all the samples ( Figure 1A). On the basis of detecting significant differences in expression in different groups using various methods, we further identified six differentially expressed miRNAs (DEMs) between the inoculated roots (R_48h_F) and the control (mock-inoculated) roots (R_48h_C) at 48 h post-inoculation, and seven DEMs between the inoculated roots (R_96h_F) and the control roots (R_96h_C) at 96 h post-inoculation, including two overlap DEMs in the two compare combinations ( Figure 1B). The expression of these 11 independent miRNAs was significantly induced by Foc with the expression value from the different sequencing libraries visualized according to the changing colors ( Figure 1C), suggesting these DEMs may be important for cucumber defense responses to FW.

Validation of miRNA Expression by qRT-PCR
To validate the identified DEMs, we completed a qRT-PCR assay to analyze the miRNA expression levels in the Foc-inoculated and control cucumber roots at three post-inoculation time-points (0, 48 and 96 h). The expression trends of the 11 selected miRNAs revealed by qRT-PCR were consistent with the sRNA sequencing data, and further reflected the reliability of the sRNA sequencing data obtained in this study ( Figure 2). Among them, the five miRNAs contained the miR6300, miR398, miR398-3p miR319a and miR319a-3p had higher expression levels relative to other miRNAs and were obviously induced after inoculation with Foc. We aimed to use the FW-resistant cultivar 'Rijiecheng' to identify the Biomolecules 2021, 11, 1620 5 of 12 resistant genes, and the accumulating evidence showed that miRNAs negatively regulate the corresponding target genes to defend against the disease infection [20]. Hence, we selected the miR319a and miR6300 that had high expression value and were significantly down-regulated expression relative to the mock-inoculated controls, suggesting that they might regulate the increased expression of defense-related genes to enhance the resistance of cucumber cultivar 'Rijiecheng' to FW.

Validation of miRNA Expression by qRT-PCR
To validate the identified DEMs, we completed a qRT-PCR assay to analyze the miRNA expression levels in the Foc-inoculated and control cucumber roots at three postinoculation time-points (0, 48 and 96 h). The expression trends of the 11 selected miRNAs revealed by qRT-PCR were consistent with the sRNA sequencing data, and further reflected the reliability of the sRNA sequencing data obtained in this study (Figure 2). Among them, the five miRNAs contained the miR6300, miR398, miR398-3p miR319a and miR319a-3p had higher expression levels relative to other miRNAs and were obviously induced after inoculation with Foc. We aimed to use the FW-resistant cultivar 'Rijiecheng' to identify the resistant genes, and the accumulating evidence showed that miRNAs negatively regulate the corresponding target genes to defend against the disease infection [20]. Hence, we selected the miR319a and miR6300 that had high expression value and were significantly down-regulated expression relative to the mock-inoculated controls, suggesting that they might regulate the increased expression of defense-related genes to enhance the resistance of cucumber cultivar 'Rijiecheng' to FW.

Identification of miRNA Target Genes and Profile Analyses
To further clarify the miRNA-mediated regulatory networks in cucumber inoculated with Foc, we predicted the target genes of miRNAs using psRobot_tar in psRobot [25]. The 1147 target genes identified for the 11 DEMs were subsequently analyzed on the basis of the transcriptome data for the 'Rijiecheng' samples inoculated with Foc. The miR319a and miR6300 respectively possessed 51 and 501 target genes, and among them, 273 genes were further performed classification based on the functional analysis of the GO enrichment with the p value < 0.05 and were mainly classified in 14 groups (e.g., protein binding, protein kinase activity, transcription factors, oxidoreductase activity) associated with diversity functions (http://cucurbitgenomics.org/goenrich, accessed on 7 September 2021). However, the function characteristics of other genes were not annotated (Figure 3, Additional file 2: Table S2). Based on the screening of differentially expressed genes via transcriptome of cucumber cultivar 'Rijiecheng' infected with Foc, we identified that 21 target genes were obviously affected by Foc. We also analyzed the association between miR6300 and miR319a and their target genes via a functional characterization based on the bioinformatics method ( Figure 4A, Additional file 3: Table S3). Moreover, the expression of these 21 target genes was significantly up-regulated induced by Foc with the expression data from the different transcriptome libraries visualized according to the changing colors, in contrast to the miRNA expression trends ( Figure 4B), indicative of a positive role for these up-regulated genes in cucumber cultivar 'Rijiecheng' defense responses to FW. Biomolecules 2021, 11, x FOR PEER REVIEW 6 of 12

Identification of miRNA Target Genes and Profile Analyses
To further clarify the miRNA-mediated regulatory networks in cucumber inoculated with Foc, we predicted the target genes of miRNAs using psRobot_tar in psRobot [25]. The 1147 target genes identified for the 11 DEMs were subsequently analyzed on the basis of the transcriptome data for the 'Rijiecheng' samples inoculated with Foc. The miR319a and miR6300 respectively possessed 51 and 501 target genes, and among them, 273 genes were further performed classification based on the functional analysis of the GO enrichment with the p value < 0.05 and were mainly classified in 14 groups (e.g., protein binding, protein kinase activity, transcription factors, oxidoreductase activity) associated with diversity functions (http://cucurbitgenomics.org/goenrich, accessed on 7 September 2021). However, the function characteristics of other genes were not annotated (Figure 3, Additional file 2: Table S2). Based on the screening of differentially expressed genes via transcriptome of cucumber cultivar 'Rijiecheng' infected with Foc, we identified that 21 target genes were obviously affected by Foc. We also analyzed the association between miR6300 and miR319a and their target genes via a functional characterization based on the bioinformatics method ( Figure 4A, Additional file 3: Table S3). Moreover, the expression of these 21 target genes was significantly up-regulated induced by Foc with the expression data from the different transcriptome libraries visualized according to the changing colors, in contrast to the miRNA expression trends ( Figure 4B), indicative of a positive role for these up-regulated genes in cucumber cultivar 'Rijiecheng' defense responses to FW.     Genes more highly or more weakly expressed in the roots were colored red and blue, respectively. The RNA-seq data were submitted to the NCBI database (accession number PRJNA472169).

Verification of the Expression of Target Genes in Cucumber Infected with Foc
To confirm the Foc-induced expression of candidate target genes, the expression patterns revealed by the transcriptome analysis of cucumber roots infected with Foc were investigated by qRT-PCR analysis. We found that the expression of 21 target genes of two miRNAs were up-regulated after the Foc inoculation relative to the mock-inoculated controls, and these genes were highly or weakly expressed in the roots colored red and blue, respectively ( Figure 5A). In detail, among them, the JRL3 gene with a putative target site of miR319a as well as the BEE1, DAHP1, and PERK2 genes with a putative target site of miR6300 were better detected by a miRNA-target gene matching analysis. Additionally, these four genes had higher expression levels relative to the others and were significantly up-regulated affected by Foc at certain post-inoculation time-points, relative to the control levels ( Figure 5B), indicating that these miRNA-target pairs might participate in cucumber defenses against FW. respectively ( Figure 5A). In detail, among them, the JRL3 gene with a putative target site of miR319a as well as the BEE1, DAHP1, and PERK2 genes with a putative target site of miR6300 were better detected by a miRNA-target gene matching analysis. Additionally, these four genes had higher expression levels relative to the others and were significantly up-regulated affected by Foc at certain post-inoculation time-points, relative to the control levels ( Figure 5B), indicating that these miRNA-target pairs might participate in cucumber defenses against FW. Figure 5. Validation of the target genes in cucumber inoculated with Foc. (A) Target gene expression levels in the Focinoculated cucumber roots and the mock-inoculated control roots were calculated according to the 2 −ΔΔCt method. Genes more highly or more weakly expressed in the roots were colored red and blue, respectively; (B) Expression patterns of four candidate target genes as well as the matches between miR6300 and miR319a and selected target genes. Data are presented as the mean ± standard error of three biological replicates. *: significantly different at p < 0.05; **: significantly different at p < 0.01.

Discussion
Fusarium wilt is a serious factor restricting global cucumber productivity [2,3]. The most sustainable method to control FW disease is the use of resistant cultivars to mine disease-resistance genes and better investigate disease-resistance mechanisms to develop FW-resistant cucumber cultivars.
Increasing research attention focus on the utility of functional miRNAs that directly regulate target genes for modulating host immune responses to enhance fungal disease resistance. For example, cotton ghr-miR164 cleaves GhNAC100 mRNA to regulate the expression of downstream disease-related genes and increase plant resistance to Verticillium wilt [19]. Additionally, in cotton, miR5272a-GhMKK6, miR414c-GhFSD1 and miR477- Figure 5. Validation of the target genes in cucumber inoculated with Foc. (A) Target gene expression levels in the Focinoculated cucumber roots and the mock-inoculated control roots were calculated according to the 2 −∆∆Ct method. Genes more highly or more weakly expressed in the roots were colored red and blue, respectively; (B) Expression patterns of four candidate target genes as well as the matches between miR6300 and miR319a and selected target genes. Data are presented as the mean ± standard error of three biological replicates. *: significantly different at p < 0.05; **: significantly different at p < 0.01.

Discussion
Fusarium wilt is a serious factor restricting global cucumber productivity [2,3]. The most sustainable method to control FW disease is the use of resistant cultivars to mine disease-resistance genes and better investigate disease-resistance mechanisms to develop FW-resistant cucumber cultivars.
Based on an earlier examination of germplasm resources inoculated with Foc, we had determined that the cucumber cultivar 'Rijiecheng' was highly resistant against Foc and might possess the resistant genes performing Foc defense [21]. Hence, we performed a comparative analysis of miRNA and transcript profiles in cucumber cultivar 'Rijiecheng' roots inoculated with Foc, we wanted to identify the DEMs and their target genes related to the FW responsive to better investigate the resistant mechanism of the cultivar 'Rijiecheng'. In detail, we identified 11 independent DEMs from the miRNA sequencing (Figure 1), we further analyzed the expression patterns of these miRNAs in the Rijiecheng roots infected with Foc ( Figure 2). Accumulating evidence shows that miRNAs negatively regulate the corresponding target genes to defend against the disease infection [20,31,32]. Therefore, we selected the miR319a and miR6300 that had high expression levels and were obviously down-regulated after Foc infection, suggesting that these two miRNAs might affect the up-regulated expression of their target genes in the cucumber cultivar Rijiecheng to defense FW disease. The target genes of miR319a and miR6300 were further screened by the transcriptome of Rijiecheng roots infected with Foc, and found that 21 genes differentially expressed after Foc attack (Figure 4). We further verified the disease-related variations in miRNAs and target genes, and revealed that the expression levels of miR319a and miR6300 and their target genes JRL3, BEE1, DAHP1 and PERK2 are obviously affected by Foc (Figures 2 and 5). Furthermore, the regulatory networks or functions of these two modules will be further investigated with phenotypes by the cucumber transgenic method. The other up-regulated miRNAs and their target genes will be further verified in the FW-susceptible cultivars and better identify the susceptibility genes to analyze the molecular mechanism of susceptibility of cucumber against FW. In the future, the availability of efficient multiplex transgene-free systems using new genome editing tools will be performed to introduce the broad-spectrum FW resistance by targeting multiple susceptibility genes simultaneously.
The miR319a-JRL3, miR6300-BEE1, miR6300-DAHP1 and miR6300-PERK2 pairs were confirmed that were obviously induced by the Foc. Additionally, the accumulated evidence shows that JRL3 contains three jacalin-like lectin domains, and additionally, the effects of JRL3 expression on plant defenses against pathogens have been investigated in several species. For example, in wheat (Triticum aestivum), TaJRLL1 encodes mannosespecific jacalin-like lectin domains and regulates the salicylic acid-dependent and jasmonic acid-dependent pathways during defense responses to the fungal pathogen Fusarium graminearum and the biotrophic fungal pathogen Blumeria graminis [52]. The BEE1 gene (brassinosteroid enhanced expression 1) encodes a bHLH domain. In soybean (Glycine max), the expression of a bHLH transcription factor gene, GmPIB1, is significantly induced by Phytophthora sojae, which results in the repressed expression of GmSPOD1 and enhanced reactive oxygen species production to increase plant resistance to P. sojae [53]. DAHP1 encodes a 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase and significantly induce by pathogenic Pseudomonas syringae strains [54]. Considered together, these findings indicate that miR319a and miR6300 might affect JRL3, BEE1, DAHP1 and PERK2 expression accordingly to regulate the resistance of cucumber to FW. This information may be relevant for future studies conducted to elucidate the genetic basis of FW resistance, with implications for the breeding of FW-resistant cucumber cultivar.

Conclusions
Based on a comparative analysis of sRNA-seq and RNA-seq data from the FW-resistant cucumber cultivar 'Rijiecheng' inoculated with Foc, we identified the Foc-responsive miRNAs and their target genes. Among them, miR319a and miR6300 were highly downregulated expression by the inoculation with Foc, and on the basis of RNA-seq analysis, 21 corresponding target genes were screened in cucumber roots inoculated with Foc. We further determined that JRL3, which is putatively targeted by miR319a, and BEE1, DAHP1 and PERK2, which are putatively targeted by miR6300, were abundantly expressed, with expression levels that were higher than those in the controls. These results revealed that miR319a-JRL3, miR6300-BEE1, miR6300-DAHP1 and miR6300-PERK2 pairs might be important for cucumber defenses against FW.
Supplementary Materials: The following are available online at https://www.mdpi.com/article/ 10.3390/biom11111620/s1, Additional file 1: Table S1. Information regarding the PCR primers, Additional file 2: Table S2. Functional classification of the targets of miR6300 and miR319a. Additional file 3: Table S3. Functional characterization of miR6300 and miR319a and differentially expressed target genes.
Author Contributions: Experiments were designed by X.C. Experiments were performed by J.X., Q.X., N.Z., K.W., X.Z., Y.L. and J.D.; J.X. and X.C. drafted and revised the manuscript. All authors have read and agreed to the published version of the manuscript.

Data Availability Statement:
The miRNA-Seq data associated with this study have been submitted to the NCBI SRA database (accession number PRJNA760453). Ethics approval and consent to participate, not applicable.

Conflicts of Interest:
All the authors declare that they have no competing interest.