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Article

The GDP-Bound State of Mitochondrial Mfn1 Induces Membrane Adhesion of Apposing Lipid Vesicles through a Cooperative Binding Mechanism

1
Dto. Química Física, Universidad Complutense de Madrid, Avenida Complutense s/n, 28040 Madrid, Spain
2
Instituto de Investigación Biomédica Hospital Doce de Octubre (imas12), Avenida de Córdoba s/n, 28041 Madrid, Spain
*
Authors to whom correspondence should be addressed.
Biomolecules 2020, 10(7), 1085; https://doi.org/10.3390/biom10071085
Received: 9 May 2020 / Revised: 10 July 2020 / Accepted: 17 July 2020 / Published: 21 July 2020
Mitochondria are double-membrane organelles that continuously undergo fission and fusion. Outer mitochondrial membrane fusion is mediated by the membrane proteins mitofusin 1 (Mfn1) and mitofusin 2 (Mfn2), carrying a GTP hydrolyzing domain (GTPase) and two coiled-coil repeats. The detailed mechanism on how the GTP hydrolysis allows Mfns to approach adjacent membranes into proximity and promote their fusion is currently under debate. Using model membranes built up as giant unilamellar vesicles (GUVs), we show here that Mfn1 promotes membrane adhesion of apposing lipid vesicles. The adhesion forces were sustained by the GDP-bound state of Mfn1 after GTP hydrolysis. In contrast, the incubation with the GDP:AlF 4 , which mimics the GTP transition state, did not induce membrane adhesion. Due to the flexible nature of lipid membranes, the adhesion strength depended on the surface concentration of Mfn1 through a cooperative binding mechanism. We discuss a possible scenario for the outer mitochondrial membrane fusion based on the modulated action of Mfn1. View Full-Text
Keywords: mitochondria; membrane fusion; GTPase; mitofusin 1; giant vesicles mitochondria; membrane fusion; GTPase; mitofusin 1; giant vesicles
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MDPI and ACS Style

Tolosa-Díaz, A.; Almendro-Vedia, V.G.; Natale, P.; López-Montero, I. The GDP-Bound State of Mitochondrial Mfn1 Induces Membrane Adhesion of Apposing Lipid Vesicles through a Cooperative Binding Mechanism. Biomolecules 2020, 10, 1085. https://doi.org/10.3390/biom10071085

AMA Style

Tolosa-Díaz A, Almendro-Vedia VG, Natale P, López-Montero I. The GDP-Bound State of Mitochondrial Mfn1 Induces Membrane Adhesion of Apposing Lipid Vesicles through a Cooperative Binding Mechanism. Biomolecules. 2020; 10(7):1085. https://doi.org/10.3390/biom10071085

Chicago/Turabian Style

Tolosa-Díaz, Andrés, Víctor G. Almendro-Vedia, Paolo Natale, and Iván López-Montero. 2020. "The GDP-Bound State of Mitochondrial Mfn1 Induces Membrane Adhesion of Apposing Lipid Vesicles through a Cooperative Binding Mechanism" Biomolecules 10, no. 7: 1085. https://doi.org/10.3390/biom10071085

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